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Gene information
General information
TC001568 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3583270..3591526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001568
    iBeetle screen iB_03209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001568 RA
      MKEEILAAV MFFIRFIEK SDKFPQDQI 
      ENFKKHLTE LLMERFEKH WFPELPTKG 
      QAYRCIRIN GVSPIDLTL ERAASKCGS 
      SYSDLRLPT ELTVWVDPS EVCYRLGES 
      EGSYCTLAT FPSENRSSS SENSTPNST 
      PPSTPLQEK LLRKVQRPP YQRYSTDFP 
      NKYRNPQNM FISPFRVKT AHEGNFNLG 
      SNRQLCRGS YPTNPPFYK NMNMNRTHY 
      KNVCRV
    CDS sequence
    • >TC001568 RA
      atgaaggaa gaaatttta gctgctgtt atgttcttt 
      ataaggttc attgagaaa agcgacaag ttccctcaa 
      gaccaaatc gaaaatttc aaaaagcac ctaaccgaa 
      cttctcatg gaaagattt gagaagcat tggtttccg 
      gaactgcca accaagggt caggcatac cgatgcatc 
      cgcatcaac ggtgtcagc cccatcgat ttgacctta 
      gaacgtgct gccagcaaa tgtggttcg tcctacagc 
      gatcttcga ttgcccact gaactgacg gtgtgggtt 
      gaccctagt gaagtatgc tacagactt ggcgaatcg 
      gaaggttcg tactgtacg ttagctact ttcccatca 
      gagaaccgt tcttcctca tctgagaat tcgactcca 
      aattcaact cctccttcg actcctctc caggaaaaa 
      ctgttacgt aaagtacaa agaccaccg tatcaaagg 
      tattcaacc gactttccg aataaatat cgtaacccc 
      cagaacatg ttcatctcg ccgtttcgg gtcaagaca 
      gcgcatgag gggaatttc aatttggga tccaatcgt 
      cagctgtgc agaggctcc tatcccacg aacccaccg 
      ttctataaa aacatgaat atgaataga acgcactac 
      aagaacgta tgtagagtg tag
    mRNA sequence
    • >TC001568 RA
      tttgatacg agacgttgc aataatacg gtgataagg 
      ttttatttt agagcgtaa caaacaact cggctttag 
      ataagaggg tgttatcag tttgggctc caaaattca 
      tcaactgtc ttattaaaa tcgtcgata atcagcccg 
      aataacgtg ttttttaaa atcgcgtga cgtcgattt 
      caaattgcg cgcgctagg aaacagatg atttctgac 
      ttgtgcttt tgttgtgac tcacatccg attcgtgtc 
      ctaatactg tactggttt gttgattct cgccttgtt 
      ttttcgaaa atctcttca aaatttgtg ttttcgatg 
      ccagttttt tccctagtg cttctgtac atcaccgtc 
      cacgaattc agataacct ttggtaact ttcattcgg 
      tttacgtct tcattttct cccctgatg tgaaggatt 
      ttgttagaa tacgaaatg aaggaagaa attttagct 
      gctgttatg ttctttata aggttcatt gagaaaagc 
      gacaagttc cctcaagac caaatcgaa aatttcaaa 
      aagcaccta accgaactt ctcatggaa agatttgag 
      aagcattgg tttccggaa ctgccaacc aagggtcag 
      gcataccga tgcatccgc atcaacggt gtcagcccc 
      atcgatttg accttagaa cgtgctgcc agcaaatgt 
      ggttcgtcc tacagcgat cttcgattg cccactgaa 
      ctgacggtg tgggttgac cctagtgaa gtatgctac 
      agacttggc gaatcggaa ggttcgtac tgtacgtta 
      gctactttc ccatcagag aaccgttct tcctcatct 
      gagaattcg actccaaat tcaactcct ccttcgact 
      cctctccag gaaaaactg ttacgtaaa gtacaaaga 
      ccaccgtat caaaggtat tcaaccgac tttccgaat 
      aaatatcgt aacccccag aacatgttc atctcgccg 
      tttcgggtc aagacagcg catgagggg aatttcaat 
      ttgggatcc aatcgtcag ctgtgcaga ggctcctat 
      cccacgaac ccaccgttc tataaaaac atgaatatg 
      aatagaacg cactacaag aacgtatgt agagtgtag 
      tagttcgtc tcatctttt tttctcaag attgattaa 
      tggtagatg gtcattgtt catgattaa gtcaccagc 
      tcccatcag gaataaaac taaaatatt tgcaaagta 
      tacaaaata cttccttcg gaacgaact ggtgcttta 
      aaatttaca cttttatat gtatagagt tcaggtgcc 
      ttcgtgtca ttatctgta tattttgta atttcatca 
      tattttaat cgagtccaa actataagc agtttgttt 
      ttaggtatt atgagaact tttatttcg taattttaa 
      gtttgttgc tgtgataaa cttctgtca tgacagata 
      atgccattt gcaatattt tgcattttc atttaaggt 
      tttctctaa ggaaactct cactcacga taattaaaa 
      cgttaaatc aacaatagt ttgctttac ttatattaa 
      gtatgtata tttgagatt agttccttt agttgttaa 
      aactcacgt gcagttctt ttaatcatt gtgagtgac 
      ccgttttcg atcgaatct gcatattgt gattagctt 
      taatcttaa agaattcta taattttcc acaactaag 
      tcatgtgat tttgtgtac atatttgac tgaattttt 
      tatcgtaat tgccaaaaa ttctaggag tatctgttt 
      gtgtaaaat gacatgctt atagtttga cagttgaag 
      tacaaattg ttggaatcg ttcaaaatg cgcatattg 
      tttttcatc atttgtata attgtcttc gtcttgtat 
      atgttctct tgtatataa ttgtaaatg tgttttttg 
      tatttctgt cggtagtca tcgggaagc aattcccac 
      caacgcatt tgtgatcat ttgactttc atctatcgt 
      taatctatg aaagttgct tgttttaac gaaacgctt 
      gatttaagg atttgtact tgtaatttc tctcgtact 
      attatttta taaagttat tcatagttt ttaagttct 
      cattatggt atgattagt attcaaatg gttcaatta 
      ttttcaatt ctcttggcg atatcgcat ccaccagtc 
      atgtttcca aatctccgt ggtccaatt acatcgtct 
      ctgtagtgt atctgtctg taaattaat tttgtacag 
      tagagttat tatgatagg aatatttgg aaatgtatg 
      tggtacaga attatccta aggtattaa tgtctaatt 
      tagtctacg cgtaatgta acttcgact gataatggt 
      acaaactag attgtgatt aaagcagtg gtgttatac 
      gaaaatggt tatgttact taatgacat ttttaatga 
      ttctgtacg atacataga tgctaggtt tggttctaa 
      ggttgatta atacatgga ttatttgaa tatcaaaca 
      ttaaaacga agactaaca gggtttcag agcatgatg 
      gattagcaa aatgaggcc taacataga aatttttat 
      aatgaattt ttgtaattt tttacttcg cttgcgcta 
      atttgcatt taattttta attagagaa tgatggttt 
      catttaatt aatccacct gttacaggt ttgtattag 
      atattttaa acaaacgga tttctggga attacgtac 
      aagttgtct ctcgtcgca gcaagtgca attaaaatt 
      tgaacaatt gtgatattt acttctaaa ctccaaatt 
      tttaactaa attttacaa cacgtatgt aattctttg 
      aagtaccta cacttttct gtagaaatt gtatctagg 
      atattagtg atgtaatat attgtgtag ccatagaaa 
      tatattaat gttaaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY