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Gene information
General information
TC001556 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2367667..2380209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001556
    iBeetle screen iB_03207
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001556 RA
      MWRLCLIFL TLGLTIIDA QRRNYSQFP CPSRCVCFR 
      RTVRCMQLE LTEVPKAPL PTVMLDLRF NKIRDIVPG 
      SFKGHKALR SLLLNNNSL KSLKNGIFT GLVQLRHLY 
      LYKNRIKYI EDEVFHGLP KLEHLYLHN NELEEFKSG 
      TFSNIPQLD RLFLYNNHI SHIPAGAFQ NLPKLTRLR 
      LDQNALVCD CRIAWFAKM LNDNTIHVA ANCRYPHEM 
      YGKPLKGMT TRDFHCKAP EIMEGPQDV EISWGSTAV 
      FTCRVTGDP KPSIYWMRD DKEIEMNND KYDIMDNGS 
      LVIKHTDES DSGHYECMA KNEDGEVKS RPARMILVR 
      PDQQFRGGN GSPVFLVTP VSVTTSESD PSVSLHCKA 
      FGNPHPTIT WSRNGIQLT TSQRHFIDE DGTLVIRPV 
      KASDHGTYR CDATNSNGR ISSEADVIV NVAPVFTVH 
      PQNTETTPG STIKLECFA VGSPPPEIT WFKDDLEVR 
      SDGRLSISP DGSSLQIHN AKETDSGLY ICEARNHLG 
      FREVSAKVK VTPLARKPP KFIYKPYNI EALVGSTIE 
      LPCKADGDP TPGIQWQKD GASLQRTGR TKVSLIGNL 
      YIYGVVPDD QGRYECAAI NDFGRSTAS CYVTVKENQ 
      NPTDFGIGD KFVKIAFDE ASHEVDKAI NKTIDNLFH 
      NKGPHSSAD LFRLVRFPD APARELARA AEVYERTLV 
      NIRKHVERG MTMNASSNF NYREILSPD HLNLVAELS 
      GCMTHRLAR NCSDMCFHS KYRTIDGTC NNLQHPMWG 
      ASLTGFRRI LKPIYENGF STPVGWNKG VKYYGFPKP 
      SSRLVSTTL IATKRTTPD GEITHMVMQ WGQFLDHDL 
      DHAIPSVSS ESWDGIDCK KSCDYAAPC YPMDVPPND 
      PRVTNRRCI DFIRSSAIC GSGMTSVFF DNIQHREQI 
      NQLTSYIDA SQVYGFSEE LARDLRDLN SDGGRLREG 
      PIFPNRKPL LPYAGNQGV DCRRNLSES TINCFVAGD 
      IRANEQAGL IAMHTLWMR EHNRVAREL KQLNPQWNS 
      DTVYHESRK IIGAAMQHL TYQHWLRFI IGEEGMQLL 
      GEYKGYDPT VNPSISNVF ATAALRFGH TLINPVLHR 
      LDWDFKPIR EGHLPLHKA FFSPWRIVD EGGIDPLLR 
      GLFTVPAKI KKPDENLNT ALTEQLFET AHAVALDLA 
      AMNIHRSRD HAIPGYIEF RKFCNMTQV DSFEDLTGE 
      ITDRSVLRK LQDLYGHPG NIDVWVGGV LEDPVKGGR 
      VGPLFRCLL IEQFRRLRD GDRFYYENP SVFKPEQLV 
      QIKQYSLSR VLCDNGDNI TRVSKNAFV LPELQGGFS 
      QCEEIPRVD LSVWSECCS DCRYSGQLN TISRLNARN 
      RRHLDEGFA NSTLKDGYF EENRKLKKR IVRMEKDLT 
      SMKDTIQEL ERVIRKLER EN
    CDS sequence
    • >TC001556 RA
      atgtggcga ttgtgtttg atttttctc acactagga 
      ctcaccatc attgacgca caaagacgg aattattca 
      caattccct tgcccttcg agatgtgtc tgcttcagg 
      agaacagtg agatgcatg caactggag ctgaccgaa 
      gtcccaaaa gcaccactg cccacagtc atgctagac 
      ctgcgtttt aacaagatt agagacatc gttcctggc 
      agttttaaa ggccataag gcccttcgt tctctacta 
      cttaataac aactcgctg aaaagtctg aaaaatggg 
      attttcacc ggtcttgtg caacttcga cacttgtat 
      ctttacaaa aatcggata aagtatatc gaagatgaa 
      gtctttcac ggactacct aaactagaa catttatac 
      ttgcacaat aacgagctg gaagaattc aaaagcgga 
      actttttcg aacattccg cagctggac agactcttc 
      ctttacaac aaccacata agtcatatc ccagcagga 
      gctttccag aacctccca aaactcact cgtttacgt 
      ctggaccaa aacgcccta gtctgcgat tgtcgaatc 
      gcctggttc gcaaaaatg ctaaacgac aacacaatt 
      cacgtcgct gctaattgt cggtacccc catgaaatg 
      tacgggaag cccctcaaa gggatgacc acgcgcgat 
      tttcattgt aaagcgcca gagatcatg gagggccca 
      caagacgtt gaaatctcg tggggaagc accgctgtt 
      ttcacttgc agggtgaca ggcgacccc aagccgtcc 
      atttattgg atgcgtgac gacaaagag attgagatg 
      aataacgac aagtatgac attatggac aacggctcg 
      ttggtgatc aaacacact gatgagagc gatagcggg 
      cattatgag tgtatggct aagaatgag gacggtgag 
      gtcaagtct agacctgcg aggatgatt ctggtacgg 
      ccagatcaa cagttccgg gggggaaat ggtagtccg 
      gtgtttctt gtaactcca gtcagtgtt acaacgagt 
      gagtcagac ccttcggtt agtttacat tgcaaggct 
      tttggtaat ccacatcct accataaca tggtctcgg 
      aatgggata caactgacg acatctcag agacatttt 
      atcgatgaa gatgggact ttggtaatt cgaccagtt 
      aaagcaagt gaccatggt acttaccgg tgcgatgct 
      accaattcc aatgggagg ataagctca gaagcggat 
      gtgatagta aatgtcgcc ccagtcttc accgtccac 
      ccccaaaac accgaaacc acccccggc tccaccatc 
      aaactcgaa tgcttcgct gtcggctcc cccccaccc 
      gaaatcacc tggttcaaa gacgacctg gaagtccgc 
      tccgacggc cgcctttcc atctcccca gacggaagc 
      tccctacaa atccacaac gccaaagaa accgactca 
      ggcctctac atatgcgaa gcccgcaac cacctcggc 
      ttccgcgaa gtcagcgcc aaagtcaaa gtcacccca 
      ctcgcccgc aaaccgcca aaattcata tacaaaccc 
      tacaacatc gaagccctg gtcggaagc accattgaa 
      ttaccctgt aaagcagac ggcgatccc acacccgga 
      atccaatgg cagaaagat ggggctagt ttgcagcga 
      actggacga accaaagtc tcactgatt gggaacttg 
      tacatctat ggagttgtt ccagacgac caaggacgc 
      tatgaatgt gctgccatt aacgatttt ggtcgaagt 
      acggcttcc tgttatgtc acagttaag gaaaaccag 
      aacccgact gatttcggt attggggac aagtttgtc 
      aaaatcgcc tttgacgaa gcttcccat gaagtggac 
      aaggccatt aacaagacc attgataat ttgtttcac 
      aacaaggga ccacattct tccgccgat ttgttccga 
      cttgttcgt ttcccagac gccccagcg cgtgaatta 
      gcacgggct gccgaagtc tacgaacgc actttagtc 
      aacattcgt aaacacgtc gaacgtggg atgacaatg 
      aacgccagt tccaatttc aactaccgg gagattctc 
      tcccctgat cacttgaac ctagtggcg gaattgtcc 
      ggttgtatg acccacaga ctggcccgc aattgctca 
      gacatgtgt ttccactca aaataccgt accattgat 
      ggtacttgc aacaacttg cagcacccc atgtggggg 
      gcgtccctg accggcttc cgccgaata ttaaagcca 
      atctacgag aatggtttc agtacccca gttggttgg 
      aacaaaggg gttaaatat tacgggttc cccaagccc 
      agctcccgg ttggtttca accacgctg atcgccacc 
      aagcgaacg acccctgac ggggaaatc acccacatg 
      gtcatgcaa tgggggcag ttcctcgac catgattta 
      gaccatgcc ataccatcg gtaagttcc gaaagctgg 
      gacggaatc gattgtaag aaatcgtgc gattacgcc 
      gccccttgc taccccatg gacgtcccc cccaacgac 
      ccccgggtc accaaccga cgctgcatc gatttcatc 
      cgatcttcg gcgatttgt ggctcgggc atgacgtcc 
      gtcttcttc gacaacatc caacaccgg gagcaaatc 
      aaccaactg acttcgtac attgacgcc tcccaagtg 
      tacggtttt tccgaagag ttggcccga gatttgcgc 
      gacttgaac agtgacggt ggccggctc cgtgaaggc 
      cccattttc cccaaccgc aaacccctg ttaccctac 
      gccgggaac cagggggtt gactgccgc agaaacttg 
      tccgaaagc acgatcaat tgcttcgtc gctggtgat 
      attcgcgca aatgagcaa gctggcctt atcgccatg 
      catacgcta tggatgcgg gagcacaac cgagtggcc 
      cgtgagttg aaacaactc aacccgcag tggaacagt 
      gatacggtc taccacgag tcccgcaaa atcataggt 
      gcggccatg caacaccta acttaccag cactggctc 
      aggtttata ataggcgag gaagggatg caacttttg 
      ggcgaatat aagggttac gacccgact gttaacccc 
      agcatttcc aatgttttt gcaacggcg gcgctccgg 
      ttcgggcac acgctcatt aacccggtc ctgcaccgc 
      ctggactgg gactttaaa ccgatccgt gaaggccac 
      ttgccgctc cacaaggca tttttctcc ccctggcgc 
      attgtggac gagggtggc attgacccc ttattaagg 
      gggctattc acggtccca gccaagatc aaaaaaccc 
      gacgagaat ttaaacacc gctctcacg gaacaactc 
      ttcgagacg gcacatgcc gtagctttg gaccttgca 
      gctatgaac atccaccgg tccagagac cacgcaatc 
      cccggctat atcgagttc cgcaaattc tgcaacatg 
      acccaagtt gatagtttc gaggacctg acgggcgaa 
      atcacagat cgttccgtt ttgcgcaag ttgcaggat 
      ttgtacggg catccgggg aacatcgat gtgtgggtc 
      gggggtgtg ctggaggac ccggtgaaa gggggccgt 
      gtcgggccg cttttccgg tgtctttta atcgagcag 
      tttaggagg ttgagggac ggggatcgg ttttattac 
      gagaacccg tccgttttt aagccggaa cagttggtt 
      caaatcaag cagtattcc ctgagtcgg gtcttgtgc 
      gataatggg gataatatc acccgggtt tccaaaaac 
      gcgtttgtt ttgcctgag ttgcaaggg gggttcagt 
      caatgtgag gagataccg agggttgat ttgagtgtg 
      tggtcggag tgttgtagc gattgtcgg tattcgggt 
      cagttgaat actattagt aggttgaat gccaggaat 
      aggcggcat ttggatgaa gggtttgcg aattcgacg 
      ctgaaggac gggtatttt gaggagaat cgaaagttg 
      aagaagagg attgttagg atggagaag gatttgact 
      agtatgaag gatacgata caggagttg gagagagtt 
      attaggaag ttggagagg gagaattaa
    mRNA sequence
    • >TC001556 RA
      caaagcgcg tccgtacgc tcacgcgtt tctggtgtt 
      taatcgcca gcagccaac aacaaccag tctctcttg 
      agacgttgt ggagaaagg attgtgttt tataaatgt 
      gcacatctg ccgagtgac gatatttct ccacaatgt 
      ggcgattgt gtttgattt ttctcacac taggactca 
      ccatcattg acgcacaaa gacggaatt attcacaat 
      tcccttgcc cttcgagat gtgtctgct tcaggagaa 
      cagtgagat gcatgcaac tggagctga ccgaagtcc 
      caaaagcac cactgccca cagtcatgc tagacctgc 
      gttttaaca agattagag acatcgttc ctggcagtt 
      ttaaaggcc ataaggccc ttcgttctc tactactta 
      ataacaact cgctgaaaa gtctgaaaa atgggattt 
      tcaccggtc ttgtgcaac ttcgacact tgtatcttt 
      acaaaaatc ggataaagt atatcgaag atgaagtct 
      ttcacggac tacctaaac tagaacatt tatacttgc 
      acaataacg agctggaag aattcaaaa gcggaactt 
      tttcgaaca ttccgcagc tggacagac tcttccttt 
      acaacaacc acataagtc atatcccag caggagctt 
      tccagaacc tcccaaaac tcactcgtt tacgtctgg 
      accaaaacg ccctagtct gcgattgtc gaatcgcct 
      ggttcgcaa aaatgctaa acgacaaca caattcacg 
      tcgctgcta attgtcggt acccccatg aaatgtacg 
      ggaagcccc tcaaaggga tgaccacgc gcgattttc 
      attgtaaag cgccagaga tcatggagg gcccacaag 
      acgttgaaa tctcgtggg gaagcaccg ctgttttca 
      cttgcaggg tgacaggcg accccaagc cgtccattt 
      attggatgc gtgacgaca aagagattg agatgaata 
      acgacaagt atgacatta tggacaacg gctcgttgg 
      tgatcaaac acactgatg agagcgata gcgggcatt 
      atgagtgta tggctaaga atgaggacg gtgaggtca 
      agtctagac ctgcgagga tgattctgg tacggccag 
      atcaacagt tccgggggg gaaatggta gtccggtgt 
      ttcttgtaa ctccagtca gtgttacaa cgagtgagt 
      cagaccctt cggttagtt tacattgca aggcttttg 
      gtaatccac atcctacca taacatggt ctcggaatg 
      ggatacaac tgacgacat ctcagagac attttatcg 
      atgaagatg ggactttgg taattcgac cagttaaag 
      caagtgacc atggtactt accggtgcg atgctacca 
      attccaatg ggaggataa gctcagaag cggatgtga 
      tagtaaatg tcgccccag tcttcaccg tccaccccc 
      aaaacaccg aaaccaccc ccggctcca ccatcaaac 
      tcgaatgct tcgctgtcg gctcccccc cacccgaaa 
      tcacctggt tcaaagacg acctggaag tccgctccg 
      acggccgcc tttccatct ccccagacg gaagctccc 
      tacaaatcc acaacgcca aagaaaccg actcaggcc 
      tctacatat gcgaagccc gcaaccacc tcggcttcc 
      gcgaagtca gcgccaaag tcaaagtca ccccactcg 
      cccgcaaac cgccaaaat tcatataca aaccctaca 
      acatcgaag ccctggtcg gaagcacca ttgaattac 
      cctgtaaag cagacggcg atcccacac ccggaatcc 
      aatggcaga aagatgggg ctagtttgc agcgaactg 
      gacgaacca aagtctcac tgattggga acttgtaca 
      tctatggag ttgttccag acgaccaag gacgctatg 
      aatgtgctg ccattaacg attttggtc gaagtacgg 
      cttcctgtt atgtcacag ttaaggaaa accagaacc 
      cgactgatt tcggtattg gggacaagt ttgtcaaaa 
      tcgcctttg acgaagctt cccatgaag tggacaagg 
      ccattaaca agaccattg ataatttgt ttcacaaca 
      agggaccac attcttccg ccgatttgt tccgacttg 
      ttcgtttcc cagacgccc cagcgcgtg aattagcac 
      gggctgccg aagtctacg aacgcactt tagtcaaca 
      ttcgtaaac acgtcgaac gtgggatga caatgaacg 
      ccagttcca atttcaact accgggaga ttctctccc 
      ctgatcact tgaacctag tggcggaat tgtccggtt 
      gtatgaccc acagactgg cccgcaatt gctcagaca 
      tgtgtttcc actcaaaat accgtacca ttgatggta 
      cttgcaaca acttgcagc accccatgt ggggggcgt 
      ccctgaccg gcttccgcc gaatattaa agccaatct 
      acgagaatg gtttcagta ccccagttg gttggaaca 
      aaggggtta aatattacg ggttcccca agcccagct 
      cccggttgg tttcaacca cgctgatcg ccaccaagc 
      gaacgaccc ctgacgggg aaatcaccc acatggtca 
      tgcaatggg ggcagttcc tcgaccatg atttagacc 
      atgccatac catcggtaa gttccgaaa gctgggacg 
      gaatcgatt gtaagaaat cgtgcgatt acgccgccc 
      cttgctacc ccatggacg tccccccca acgaccccc 
      gggtcacca accgacgct gcatcgatt tcatccgat 
      cttcggcga tttgtggct cgggcatga cgtccgtct 
      tcttcgaca acatccaac accgggagc aaatcaacc 
      aactgactt cgtacattg acgcctccc aagtgtacg 
      gtttttccg aagagttgg cccgagatt tgcgcgact 
      tgaacagtg acggtggcc ggctccgtg aaggcccca 
      ttttcccca accgcaaac ccctgttac cctacgccg 
      ggaaccagg gggttgact gccgcagaa acttgtccg 
      aaagcacga tcaattgct tcgtcgctg gtgatattc 
      gcgcaaatg agcaagctg gccttatcg ccatgcata 
      cgctatgga tgcgggagc acaaccgag tggcccgtg 
      agttgaaac aactcaacc cgcagtgga acagtgata 
      cggtctacc acgagtccc gcaaaatca taggtgcgg 
      ccatgcaac acctaactt accagcact ggctcaggt 
      ttataatag gcgaggaag ggatgcaac ttttgggcg 
      aatataagg gttacgacc cgactgtta accccagca 
      tttccaatg tttttgcaa cggcggcgc tccggttcg 
      ggcacacgc tcattaacc cggtcctgc accgcctgg 
      actgggact ttaaaccga tccgtgaag gccacttgc 
      cgctccaca aggcatttt tctccccct ggcgcattg 
      tggacgagg gtggcattg accccttat taagggggc 
      tattcacgg tcccagcca agatcaaaa aacccgacg 
      agaatttaa acaccgctc tcacggaac aactcttcg 
      agacggcac atgccgtag ctttggacc ttgcagcta 
      tgaacatcc accggtcca gagaccacg caatccccg 
      gctatatcg agttccgca aattctgca acatgaccc 
      aagttgata gtttcgagg acctgacgg gcgaaatca 
      cagatcgtt ccgttttgc gcaagttgc aggatttgt 
      acgggcatc cggggaaca tcgatgtgt gggtcgggg 
      gtgtgctgg aggacccgg tgaaagggg gccgtgtcg 
      ggccgcttt tccggtgtc ttttaatcg agcagttta 
      ggaggttga gggacgggg atcggtttt attacgaga 
      acccgtccg tttttaagc cggaacagt tggttcaaa 
      tcaagcagt attccctga gtcgggtct tgtgcgata 
      atggggata atatcaccc gggtttcca aaaacgcgt 
      ttgttttgc ctgagttgc aaggggggt tcagtcaat 
      gtgaggaga taccgaggg ttgatttga gtgtgtggt 
      cggagtgtt gtagcgatt gtcggtatt cgggtcagt 
      tgaatacta ttagtaggt tgaatgcca ggaataggc 
      ggcatttgg atgaagggt ttgcgaatt cgacgctga 
      aggacgggt attttgagg agaatcgaa agttgaaga 
      agaggattg ttaggatgg agaaggatt tgactagta 
      tgaaggata cgatacagg agttggaga gagttatta 
      ggaagttgg agagggaga attaagtga gaatatttt 
      tagtaatcg ctgccatcg tttcgctgt taagtctag 
      agttaattt ttagcactg tactgccag gtttactta 
      aatttcacc taaatttac gcatttatt ttcttgtga 
      taaactaac aatacgtac ctacctaat cgaattgtt 
      gtaaaaata cataaataa aaaataaat taagaa