| General information | |||
| TC031029
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 3270705..3339257 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031029 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03205 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031029 RA
MGTIGPSVA AAATAVVEA ATFVLLLLA APHTDALEL GSCGSALGM ESGAIKDSA ITASSAYDS SSVGPQHGR LRNDKNGGA WCPRQMVSR DAKEYLEIN LEELHVITG CRTQGRFGN GQGQEYAEE YMIEYWRPN FTKWVRWKN RSGKELLPG NTNTFTIVD QKLDPPVIA SKIRFVPYS DHVRTVCMR TELIGCIWT DGLLSYDVP QGLHRGSEL DLTDRTYDG REEGGLLQR GLGQLVDGQ KGQDNFRLD LAGHGKGYE WVGWRNDTP GWDGHPLEM RFEFDKVRN FSAAHLHTN NLFSKDVQV FSHARVFFS VNGNLFNGE PVHFSYMPD LVLEHARNV TIKLHHRIG RFLKLQLYF ASRWILLSE ISFDSVVIT GNFTETAED IPIPDSGKE YPLQRDEVK STSSKANRN TTGASTKSG TNEEPKHYI GFVIGILTV VILILVAAI VFIVFRNQR LKTATGLTV VPRLDKRIH CDKMAMEDC DKSALYVET CKTNLYARQ THPNSPDYS EVPDLVCQE YTVPQHMMD KSFSQSSRA PPPMLNFLP KPPPVPPPP ASYYTDQDI CSPLPLVSS PAKSSRHFM LPLSPEPED CSDQDEVDV AIAIFPREQ LAIIEKIGE GPFGDVHLC EVLADDGKL SDCKFVVVH TLRLETYRE EFNKEVKAL ARLNDPNIA RLLGACLDT EPICAVREY APMGDLCQF LQDHVAETA TPLATTAKT LSYGCLVYM ATQIASGMK YLESLRYVH KDLAARNCL VGRRYEIKV SDLGSYRRM YSSDYCQIG ESRPLPLRW MAWESVLLG KFSGKSDVW SFAVLLWEI MTFAREQPF EELSDDKVI ENVTHFYQD DGKHVLLSP PINCPKEIF DLMCECWQR NESDRPNFR EIHLFLQRK NLGYNPELH
- >TC031029 RA
atgggcact atcggacca tcggtggcg gcggcggcg acggcggtg gtggaggca gcgacgttc gtcctgttg ctccttgct gctccccac accgacgca ttggaatta ggatcatgc ggatcagct cttggaatg gagtccgga gctatcaag gattctgca ataaccgca agttctgct tatgattcc agcagtgtc ggaccgcaa catggaagg cttcgcaat gacaagaac ggaggcgcc tggtgtccg agacaaatg gtgtctcga gatgccaag gaatatttg gaaataaac ttggaagag ttgcacgtg ataacgggc tgtcgcact caaggacgc ttcggcaac ggccaagga caagaatat gccgaagag tacatgatt gagtactgg agacccaac tttaccaag tgggtgagg tggaaaaat cggtccgga aaagagctt ctaccagga aatacaaac actttcacg atcgtagat cagaagctt gatcctcct gtaatagcc tcgaaaatt cgttttgtt ccatatagc gatcacgtg cgtactgtt tgcatgagg actgaactc atcggctgc atatggaca gacggtctg ttgagctac gatgtgcct cagggactc caccgtggc tccgagttg gacctcaca gaccgcact tacgacggc cgagaagaa ggcggcctt cttcagcga gggctaggc cagctcgtc gatggccag aaaggacaa gacaacttc aggcttgac ctcgcagga catggtaaa ggttatgag tgggtggga tggcgcaac gacacacct ggttgggat ggccaccct ttagaaatg cgttttgag ttcgacaaa gtacgcaac ttctcagcc gctcatctg catactaat aatctcttc agcaaagat gtgcaggtg ttctcccat gctcgagta ttttttagt gtgaatgga aatttgttc aacggcgaa cctgttcat ttttcgtac atgccggac ctggttttg gaacacgcc cgcaacgtt acgatcaaa ctccaccac cgcatcgga cgattcctc aaacttcaa ctgtacttc gcctcccga tggatcttg ctcagtgaa atctctttt gactccgtg gtgataaca ggcaatttt acggagacg gcggaggac atcccaatc ccagattcc ggcaaggaa tatccgtta caaagagat gaagttaaa agtacgtcc agtaaagca aatcgtaat acaacggga gcttccaca aaaagcgga actaatgaa gaacctaag cactacatt ggtttcgtt attgggatc cttaccgtt gtaatattg atccttgtg gcagcgatt gtgtttatc gtctttcga aatcaacga ctcaaaacg gccacagga ctcacggtt gtgcccagg ttggacaaa aggattcat tgtgacaag atggcaatg gaagactgc gataaatca gctctttac gttgaaact tgcaagacg aatctttat gctcgacag acgcatcca aacagtccg gattattcc gaagtgccg gatctggtt tgccaggaa tacactgtt ccgcagcac atgatggac aagtcgttt tcacaatcg tcgcgagcg ccgccaccg atgctgaac ttcctgccc aaaccaccg cccgtgcct ccacctccg gcgagctac tacacggac caagatata tgcagtccc cttccgctg gtgtcatca cccgcgaaa agcagtcgc catttcatg ctgcccctg agtccggaa ccggaagat tgcagcgat caggacgag gtggacgtg gcgatcgca attttcccg cgtgaacaa ttagccata atcgaaaaa atcggcgaa ggacctttc ggagacgtg catctgtgc gaagtgctg gccgatgac ggcaaactc agtgactgc aagttcgtg gtcgtgcac acgctgcgc ttggaaact tacagagag gagtttaat aaagaggtg aaagcgctg gcgagacta aatgatccg aatattgcc cgactgctc ggcgcttgc ctcgacacg gaaccaata tgtgcagtg agagagtac gcgccaatg ggagatttg tgccaattt ttgcaagat cacgtcgct gaaacggcc actccgctc gctaccact gccaaaacg ctgagttat ggttgtctt gtttacatg gcaactcaa atagcgtct ggtatgaag tacttggag tcactgcgg tatgttcac aaggacttg gcggcgagg aactgtctg gtcgggaga cgctacgaa atcaaggta tcggatctg ggctcgtac cgacgaatg tacagctcg gattactgc caaataggg gaatcccgc cctctgccc ctgcgctgg atggcatgg gaaagcgtc ctcttagga aagttttcg ggtaaaagc gacgtctgg tcgttcgcc gttctgctg tgggaaata atgacattc gcccgagag cagcctttc gaagagttg agcgacgat aaagtgata gaaaacgtc actcacttc taccaagac gacggcaaa cacgtcttg ctgagcccg ccaatcaat tgtcccaag gagatattc gatctaatg tgcgagtgt tggcaaaga aatgaatct gacaggccc aacttccga gagatccat ttattcctg cagaggaaa aacttgggc tacaatccc gagcttcac tga
- >TC031029 RA
agactgtgc tccaaactt caaaccaca cgtgctcca aacttttca gactaaagc tccgtttaa ttcaaatcg aacgaacgc atcttaaat tacggcctg tcattgaca attacatgt gaatctgga cgattttag tgccatggt tctcgaatt attttattc ggaggagga ataaaagcg atgtgcaga agtggcgcc ggattcaca atcatccct catcagcgt cgtgaaaca caaaccgga gttaaacga ggtaacaat gagcggctg aaaagcggc ggcagtgcg tgagtgacc cgtgcgtga gtgacctgt gcgtgagtg acccgtgcg tgagctgcc atgcggccg gtggcgcga taatgacgg cggtcggca tgggcacta tcggaccat cggtggcgg cggcggcga cggcggtgg tggaggcag cgacgttcg tcctgttgc tccttgctg ctccccaca ccgacgcat tggaattag gatcatgcg gatcagctc ttggaatgg agtccggag ctatcaagg attctgcaa taaccgcaa gttctgctt atgattcca gcagtgtcg gaccgcaac atggaaggc ttcgcaatg acaagaacg gaggcgcct ggtgtccga gacaaatgg tgtctcgag atgccaagg aatatttgg aaataaact tggaagagt tgcacgtga taacgggct gtcgcactc aaggacgct tcggcaacg gccaaggac aagaatatg ccgaagagt acatgattg agtactgga gacccaact ttaccaagt gggtgaggt ggaaaaatc ggtccggaa aagagcttc taccaggaa atacaaaca ctttcacga tcgtagatc agaagcttg atcctcctg taatagcct cgaaaattc gttttgttc catatagcg atcacgtgc gtactgttt gcatgagga ctgaactca tcggctgca tatggacag acggtctgt tgagctacg atgtgcctc agggactcc accgtggct ccgagttgg acctcacag accgcactt acgacggcc gagaagaag gcggccttc ttcagcgag ggctaggcc agctcgtcg atggccaga aaggacaag acaacttca ggcttgacc tcgcaggac atggtaaag gttatgagt gggtgggat ggcgcaacg acacacctg gttgggatg gccaccctt tagaaatgc gttttgagt tcgacaaag tacgcaact tctcagccg ctcatctgc atactaata atctcttca gcaaagatg tgcaggtgt tctcccatg ctcgagtat tttttagtg tgaatggaa atttgttca acggcgaac ctgttcatt tttcgtaca tgccggacc tggttttgg aacacgccc gcaacgtta cgatcaaac tccaccacc gcatcggac gattcctca aacttcaac tgtacttcg cctcccgat ggatcttgc tcagtgaaa tctcttttg actccgtgg tgataacag gcaatttta cggagacgg cggaggaca tcccaatcc cagattccg gcaaggaat atccgttac aaagagatg aagttaaaa gtacgtcca gtaaagcaa atcgtaata caacgggag cttccacaa aaagcggaa ctaatgaag aacctaagc actacattg gtttcgtta ttgggatcc ttaccgttg taatattga tccttgtgg cagcgattg tgtttatcg tctttcgaa atcaacgac tcaaaacgg ccacaggac tcacggttg tgcccaggt tggacaaaa ggattcatt gtgacaaga tggcaatgg aagactgcg ataaatcag ctctttacg ttgaaactt gcaagacga atctttatg ctcgacaga cgcatccaa acagtccgg attattccg aagtgccgg atctggttt gccaggaat acactgttc cgcagcaca tgatggaca agtcgtttt cacaatcgt cgcgagcgc cgccaccga tgctgaact tcctgccca aaccaccgc ccgtgcctc cacctccgg cgagctact acacggacc aagatatat gcagtcccc ttccgctgg tgtcatcac ccgcgaaaa gcagtcgcc atttcatgc tgcccctga gtccggaac cggaagatt gcagcgatc aggacgagg tggacgtgg cgatcgcaa ttttcccgc gtgaacaat tagccataa tcgaaaaaa tcggcgaag gacctttcg gagacgtgc atctgtgcg aagtgctgg ccgatgacg gcaaactca gtgactgca agttcgtgg tcgtgcaca cgctgcgct tggaaactt acagagagg agtttaata aagaggtga aagcgctgg cgagactaa atgatccga atattgccc gactgctcg gcgcttgcc tcgacacgg aaccaatat gtgcagtga gagagtacg cgccaatgg gagatttgt gccaatttt tgcaagatc acgtcgctg aaacggcca ctccgctcg ctaccactg ccaaaacgc tgagttatg gttgtcttg tttacatgg caactcaaa tagcgtctg gtatgaagt acttggagt cactgcggt atgttcaca aggacttgg cggcgagga actgtctgg tcgggagac gctacgaaa tcaaggtat cggatctgg gctcgtacc gacgaatgt acagctcgg attactgcc aaatagggg aatcccgcc ctctgcccc tgcgctgga tggcatggg aaagcgtcc tcttaggaa agttttcgg gtaaaagcg acgtctggt cgttcgccg ttctgctgt gggaaataa tgacattcg cccgagagc agcctttcg aagagttga gcgacgata aagtgatag aaaacgtca ctcacttct accaagacg acggcaaac acgtcttgc tgagcccgc caatcaatt gtcccaagg agatattcg atctaatgt gcgagtgtt ggcaaagaa atgaatctg acaggccca acttccgag agatccatt tattcctgc agaggaaaa acttgggct acaatcccg agcttcact gacatctgc taatgatgt acattatcg acaataaat tgaaagaat tgcgtgata tattccacc tgtaactgt ggcgaactc gttattcta agcgcattt gttgccgct aattctagt tattatcac ttgtgcttc attcatagt acgcttaaa cctgcgttt gtgccttct attcttctt actcgactc tctattcgt tgcgtgaca atttctaat gtgatattt tatttattt tcggtgtag tgcaattgt taactgtag ccgttagtc ttaattaaa tcgtgaaac gagttgtct aagtgctta tacgtatag ttgttattg tatacggac aggtcatca agaatcgaa tttcgcttc cctttgtac aaataagtc gtttcctca taggcctaa gcagcgtta ggatttcta aattcgttc caacgaata gatttctac tcacacttt tatttttta taactcgaa atgtctagt gtaagcgat agtcattgt tcaataata tcgaagtta acattgtaa aatctttta ataaattac tgcctgtat tttcgactg aacgatagt gcaaatgca tttcttgtt catatcata tagctagcg ttaaggtgt aaatatgtc tgtacaaaa tgtatctcc cttcagttt ttatacatt aatacgatt taacataca cattgtttg ttgacactg gtaaactgg accaataaa gatattaaa actgg
