Status
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Gene information
General information
TC031029
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001550,TC001551
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3270705..3339257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031029
    iBeetle screen iB_03205

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031029 RA
      MGTIGPSVA AAATAVVEA ATFVLLLLA APHTDALEL 
      GSCGSALGM ESGAIKDSA ITASSAYDS SSVGPQHGR 
      LRNDKNGGA WCPRQMVSR DAKEYLEIN LEELHVITG 
      CRTQGRFGN GQGQEYAEE YMIEYWRPN FTKWVRWKN 
      RSGKELLPG NTNTFTIVD QKLDPPVIA SKIRFVPYS 
      DHVRTVCMR TELIGCIWT DGLLSYDVP QGLHRGSEL 
      DLTDRTYDG REEGGLLQR GLGQLVDGQ KGQDNFRLD 
      LAGHGKGYE WVGWRNDTP GWDGHPLEM RFEFDKVRN 
      FSAAHLHTN NLFSKDVQV FSHARVFFS VNGNLFNGE 
      PVHFSYMPD LVLEHARNV TIKLHHRIG RFLKLQLYF 
      ASRWILLSE ISFDSVVIT GNFTETAED IPIPDSGKE 
      YPLQRDEVK STSSKANRN TTGASTKSG TNEEPKHYI 
      GFVIGILTV VILILVAAI VFIVFRNQR LKTATGLTV 
      VPRLDKRIH CDKMAMEDC DKSALYVET CKTNLYARQ 
      THPNSPDYS EVPDLVCQE YTVPQHMMD KSFSQSSRA 
      PPPMLNFLP KPPPVPPPP ASYYTDQDI CSPLPLVSS 
      PAKSSRHFM LPLSPEPED CSDQDEVDV AIAIFPREQ 
      LAIIEKIGE GPFGDVHLC EVLADDGKL SDCKFVVVH 
      TLRLETYRE EFNKEVKAL ARLNDPNIA RLLGACLDT 
      EPICAVREY APMGDLCQF LQDHVAETA TPLATTAKT 
      LSYGCLVYM ATQIASGMK YLESLRYVH KDLAARNCL 
      VGRRYEIKV SDLGSYRRM YSSDYCQIG ESRPLPLRW 
      MAWESVLLG KFSGKSDVW SFAVLLWEI MTFAREQPF 
      EELSDDKVI ENVTHFYQD DGKHVLLSP PINCPKEIF 
      DLMCECWQR NESDRPNFR EIHLFLQRK NLGYNPELH
    CDS sequence
    • >TC031029 RA
      atgggcact atcggacca tcggtggcg gcggcggcg 
      acggcggtg gtggaggca gcgacgttc gtcctgttg 
      ctccttgct gctccccac accgacgca ttggaatta 
      ggatcatgc ggatcagct cttggaatg gagtccgga 
      gctatcaag gattctgca ataaccgca agttctgct 
      tatgattcc agcagtgtc ggaccgcaa catggaagg 
      cttcgcaat gacaagaac ggaggcgcc tggtgtccg 
      agacaaatg gtgtctcga gatgccaag gaatatttg 
      gaaataaac ttggaagag ttgcacgtg ataacgggc 
      tgtcgcact caaggacgc ttcggcaac ggccaagga 
      caagaatat gccgaagag tacatgatt gagtactgg 
      agacccaac tttaccaag tgggtgagg tggaaaaat 
      cggtccgga aaagagctt ctaccagga aatacaaac 
      actttcacg atcgtagat cagaagctt gatcctcct 
      gtaatagcc tcgaaaatt cgttttgtt ccatatagc 
      gatcacgtg cgtactgtt tgcatgagg actgaactc 
      atcggctgc atatggaca gacggtctg ttgagctac 
      gatgtgcct cagggactc caccgtggc tccgagttg 
      gacctcaca gaccgcact tacgacggc cgagaagaa 
      ggcggcctt cttcagcga gggctaggc cagctcgtc 
      gatggccag aaaggacaa gacaacttc aggcttgac 
      ctcgcagga catggtaaa ggttatgag tgggtggga 
      tggcgcaac gacacacct ggttgggat ggccaccct 
      ttagaaatg cgttttgag ttcgacaaa gtacgcaac 
      ttctcagcc gctcatctg catactaat aatctcttc 
      agcaaagat gtgcaggtg ttctcccat gctcgagta 
      ttttttagt gtgaatgga aatttgttc aacggcgaa 
      cctgttcat ttttcgtac atgccggac ctggttttg 
      gaacacgcc cgcaacgtt acgatcaaa ctccaccac 
      cgcatcgga cgattcctc aaacttcaa ctgtacttc 
      gcctcccga tggatcttg ctcagtgaa atctctttt 
      gactccgtg gtgataaca ggcaatttt acggagacg 
      gcggaggac atcccaatc ccagattcc ggcaaggaa 
      tatccgtta caaagagat gaagttaaa agtacgtcc 
      agtaaagca aatcgtaat acaacggga gcttccaca 
      aaaagcgga actaatgaa gaacctaag cactacatt 
      ggtttcgtt attgggatc cttaccgtt gtaatattg 
      atccttgtg gcagcgatt gtgtttatc gtctttcga 
      aatcaacga ctcaaaacg gccacagga ctcacggtt 
      gtgcccagg ttggacaaa aggattcat tgtgacaag 
      atggcaatg gaagactgc gataaatca gctctttac 
      gttgaaact tgcaagacg aatctttat gctcgacag 
      acgcatcca aacagtccg gattattcc gaagtgccg 
      gatctggtt tgccaggaa tacactgtt ccgcagcac 
      atgatggac aagtcgttt tcacaatcg tcgcgagcg 
      ccgccaccg atgctgaac ttcctgccc aaaccaccg 
      cccgtgcct ccacctccg gcgagctac tacacggac 
      caagatata tgcagtccc cttccgctg gtgtcatca 
      cccgcgaaa agcagtcgc catttcatg ctgcccctg 
      agtccggaa ccggaagat tgcagcgat caggacgag 
      gtggacgtg gcgatcgca attttcccg cgtgaacaa 
      ttagccata atcgaaaaa atcggcgaa ggacctttc 
      ggagacgtg catctgtgc gaagtgctg gccgatgac 
      ggcaaactc agtgactgc aagttcgtg gtcgtgcac 
      acgctgcgc ttggaaact tacagagag gagtttaat 
      aaagaggtg aaagcgctg gcgagacta aatgatccg 
      aatattgcc cgactgctc ggcgcttgc ctcgacacg 
      gaaccaata tgtgcagtg agagagtac gcgccaatg 
      ggagatttg tgccaattt ttgcaagat cacgtcgct 
      gaaacggcc actccgctc gctaccact gccaaaacg 
      ctgagttat ggttgtctt gtttacatg gcaactcaa 
      atagcgtct ggtatgaag tacttggag tcactgcgg 
      tatgttcac aaggacttg gcggcgagg aactgtctg 
      gtcgggaga cgctacgaa atcaaggta tcggatctg 
      ggctcgtac cgacgaatg tacagctcg gattactgc 
      caaataggg gaatcccgc cctctgccc ctgcgctgg 
      atggcatgg gaaagcgtc ctcttagga aagttttcg 
      ggtaaaagc gacgtctgg tcgttcgcc gttctgctg 
      tgggaaata atgacattc gcccgagag cagcctttc 
      gaagagttg agcgacgat aaagtgata gaaaacgtc 
      actcacttc taccaagac gacggcaaa cacgtcttg 
      ctgagcccg ccaatcaat tgtcccaag gagatattc 
      gatctaatg tgcgagtgt tggcaaaga aatgaatct 
      gacaggccc aacttccga gagatccat ttattcctg 
      cagaggaaa aacttgggc tacaatccc gagcttcac 
      tga
    mRNA sequence
    • >TC031029 RA
      agactgtgc tccaaactt caaaccaca cgtgctcca 
      aacttttca gactaaagc tccgtttaa ttcaaatcg 
      aacgaacgc atcttaaat tacggcctg tcattgaca 
      attacatgt gaatctgga cgattttag tgccatggt 
      tctcgaatt attttattc ggaggagga ataaaagcg 
      atgtgcaga agtggcgcc ggattcaca atcatccct 
      catcagcgt cgtgaaaca caaaccgga gttaaacga 
      ggtaacaat gagcggctg aaaagcggc ggcagtgcg 
      tgagtgacc cgtgcgtga gtgacctgt gcgtgagtg 
      acccgtgcg tgagctgcc atgcggccg gtggcgcga 
      taatgacgg cggtcggca tgggcacta tcggaccat 
      cggtggcgg cggcggcga cggcggtgg tggaggcag 
      cgacgttcg tcctgttgc tccttgctg ctccccaca 
      ccgacgcat tggaattag gatcatgcg gatcagctc 
      ttggaatgg agtccggag ctatcaagg attctgcaa 
      taaccgcaa gttctgctt atgattcca gcagtgtcg 
      gaccgcaac atggaaggc ttcgcaatg acaagaacg 
      gaggcgcct ggtgtccga gacaaatgg tgtctcgag 
      atgccaagg aatatttgg aaataaact tggaagagt 
      tgcacgtga taacgggct gtcgcactc aaggacgct 
      tcggcaacg gccaaggac aagaatatg ccgaagagt 
      acatgattg agtactgga gacccaact ttaccaagt 
      gggtgaggt ggaaaaatc ggtccggaa aagagcttc 
      taccaggaa atacaaaca ctttcacga tcgtagatc 
      agaagcttg atcctcctg taatagcct cgaaaattc 
      gttttgttc catatagcg atcacgtgc gtactgttt 
      gcatgagga ctgaactca tcggctgca tatggacag 
      acggtctgt tgagctacg atgtgcctc agggactcc 
      accgtggct ccgagttgg acctcacag accgcactt 
      acgacggcc gagaagaag gcggccttc ttcagcgag 
      ggctaggcc agctcgtcg atggccaga aaggacaag 
      acaacttca ggcttgacc tcgcaggac atggtaaag 
      gttatgagt gggtgggat ggcgcaacg acacacctg 
      gttgggatg gccaccctt tagaaatgc gttttgagt 
      tcgacaaag tacgcaact tctcagccg ctcatctgc 
      atactaata atctcttca gcaaagatg tgcaggtgt 
      tctcccatg ctcgagtat tttttagtg tgaatggaa 
      atttgttca acggcgaac ctgttcatt tttcgtaca 
      tgccggacc tggttttgg aacacgccc gcaacgtta 
      cgatcaaac tccaccacc gcatcggac gattcctca 
      aacttcaac tgtacttcg cctcccgat ggatcttgc 
      tcagtgaaa tctcttttg actccgtgg tgataacag 
      gcaatttta cggagacgg cggaggaca tcccaatcc 
      cagattccg gcaaggaat atccgttac aaagagatg 
      aagttaaaa gtacgtcca gtaaagcaa atcgtaata 
      caacgggag cttccacaa aaagcggaa ctaatgaag 
      aacctaagc actacattg gtttcgtta ttgggatcc 
      ttaccgttg taatattga tccttgtgg cagcgattg 
      tgtttatcg tctttcgaa atcaacgac tcaaaacgg 
      ccacaggac tcacggttg tgcccaggt tggacaaaa 
      ggattcatt gtgacaaga tggcaatgg aagactgcg 
      ataaatcag ctctttacg ttgaaactt gcaagacga 
      atctttatg ctcgacaga cgcatccaa acagtccgg 
      attattccg aagtgccgg atctggttt gccaggaat 
      acactgttc cgcagcaca tgatggaca agtcgtttt 
      cacaatcgt cgcgagcgc cgccaccga tgctgaact 
      tcctgccca aaccaccgc ccgtgcctc cacctccgg 
      cgagctact acacggacc aagatatat gcagtcccc 
      ttccgctgg tgtcatcac ccgcgaaaa gcagtcgcc 
      atttcatgc tgcccctga gtccggaac cggaagatt 
      gcagcgatc aggacgagg tggacgtgg cgatcgcaa 
      ttttcccgc gtgaacaat tagccataa tcgaaaaaa 
      tcggcgaag gacctttcg gagacgtgc atctgtgcg 
      aagtgctgg ccgatgacg gcaaactca gtgactgca 
      agttcgtgg tcgtgcaca cgctgcgct tggaaactt 
      acagagagg agtttaata aagaggtga aagcgctgg 
      cgagactaa atgatccga atattgccc gactgctcg 
      gcgcttgcc tcgacacgg aaccaatat gtgcagtga 
      gagagtacg cgccaatgg gagatttgt gccaatttt 
      tgcaagatc acgtcgctg aaacggcca ctccgctcg 
      ctaccactg ccaaaacgc tgagttatg gttgtcttg 
      tttacatgg caactcaaa tagcgtctg gtatgaagt 
      acttggagt cactgcggt atgttcaca aggacttgg 
      cggcgagga actgtctgg tcgggagac gctacgaaa 
      tcaaggtat cggatctgg gctcgtacc gacgaatgt 
      acagctcgg attactgcc aaatagggg aatcccgcc 
      ctctgcccc tgcgctgga tggcatggg aaagcgtcc 
      tcttaggaa agttttcgg gtaaaagcg acgtctggt 
      cgttcgccg ttctgctgt gggaaataa tgacattcg 
      cccgagagc agcctttcg aagagttga gcgacgata 
      aagtgatag aaaacgtca ctcacttct accaagacg 
      acggcaaac acgtcttgc tgagcccgc caatcaatt 
      gtcccaagg agatattcg atctaatgt gcgagtgtt 
      ggcaaagaa atgaatctg acaggccca acttccgag 
      agatccatt tattcctgc agaggaaaa acttgggct 
      acaatcccg agcttcact gacatctgc taatgatgt 
      acattatcg acaataaat tgaaagaat tgcgtgata 
      tattccacc tgtaactgt ggcgaactc gttattcta 
      agcgcattt gttgccgct aattctagt tattatcac 
      ttgtgcttc attcatagt acgcttaaa cctgcgttt 
      gtgccttct attcttctt actcgactc tctattcgt 
      tgcgtgaca atttctaat gtgatattt tatttattt 
      tcggtgtag tgcaattgt taactgtag ccgttagtc 
      ttaattaaa tcgtgaaac gagttgtct aagtgctta 
      tacgtatag ttgttattg tatacggac aggtcatca 
      agaatcgaa tttcgcttc cctttgtac aaataagtc 
      gtttcctca taggcctaa gcagcgtta ggatttcta 
      aattcgttc caacgaata gatttctac tcacacttt 
      tatttttta taactcgaa atgtctagt gtaagcgat 
      agtcattgt tcaataata tcgaagtta acattgtaa 
      aatctttta ataaattac tgcctgtat tttcgactg 
      aacgatagt gcaaatgca tttcttgtt catatcata 
      tagctagcg ttaaggtgt aaatatgtc tgtacaaaa 
      tgtatctcc cttcagttt ttatacatt aatacgatt 
      taacataca cattgtttg ttgacactg gtaaactgg 
      accaataaa gatattaaa actgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY