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Gene information
General information
TC001549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3226415..3243638
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001549
    iBeetle screen iB_03204

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001549 RA
      MKVLLSSVM ILLVTAADG VDPSQCIAP LGMESGAIK 
      DQDITASSS FEHGNVGPH HGRLRNELD GGAWCPQSA 
      ATPDLKEWI QIDLHTVHM ITATETQGR FGNGKGMEF 
      AEAYVLEYW RPRFLNKWI RYRNRSGEE ALKGNINTY 
      LESKTILDP PIWASKIKF LPLSHHERT VCMRVEIYG 
      CRWTDGIQS YSMPQGDKR GTSWEFYDT AYDGHWDGE 
      KLQYGLGQL TDGKVAPDD FKASFYQTN SQQGWVGWK 
      NDTRKNKPI EIVFEFDKV REFTAVHIY TNNQFTKDV 
      QVFSQAKVL FSVGGKRYK GEPITFDYM EDRIWETPR 
      NVSIRLYHR VGRFVKIQL QFAARWLMI SEVTFDSSV 
      VNGNFTEEE EEELEEQTL QKDQIASPG ADSKNVVSA 
      VNTPSGLGQ YLGVIVGVL SVLVVLMVA VVVFVVVQQ 
      RRYKSSPRP SQVPGGCDK AALYREPSM GRLPGSSDY 
      ADVRDPEYA VPLQPPQTP RAPPPLHNF FPKPPPVPP 
      PPERYYAAT EICNSGFAQ QTAEICLEY EDLRWTLNC 
      DKSTLRWGR LRYHYLLAP RPSFAKAED YQICLTEIS 
      YVAFTIRQS NIIRCAICV VNRDPYFKL
    CDS sequence
    • >TC001549 RA
      atgaaggtg ctcctgtcg tcggtgatg atcctgttg 
      gtgacagca gcagatggc gtcgacccc tcgcagtgc 
      atcgcgcct ctgggcatg gaatcgggc gccatcaag 
      gatcaggat attacagcc agttcttcg ttcgagcat 
      ggcaatgtg ggaccgcat cacggaagg ttgaggaac 
      gagttggat gggggagcc tggtgtcct caaagcgcc 
      gccactccc gatttgaag gagtggata caaatagac 
      ttacacacg gtccatatg attacggcg actgaaacg 
      caagggcgg ttcgggaac gggaagggg atggagttt 
      gcggaagcc tatgtcctg gaatactgg cgacctcgc 
      tttttgaat aagtggatt cggtacagg aaccgctcg 
      ggtgaagag gctctcaaa ggcaacatc aatacatac 
      ctcgagtcg aagaccatc ctggacccg cccatatgg 
      gccagtaaa atcaaattt ctgcccttg agtcaccac 
      gaaagaacg gtttgcatg cgcgtggaa atctatggg 
      tgtcgctgg accgatggt attcaaagt tattcgatg 
      ccgcaaggt gataagcgt ggaactagt tgggagttc 
      tatgacacg gcctatgat gggcactgg gacggtgag 
      aagctacaa tacggattg gggcaattg acggacggg 
      aaagtggcc cctgatgac ttcaaagcg tcgttttat 
      caaaccaat tcgcagcaa ggctgggtc gggtggaag 
      aacgacacg cgtaagaac aaaccgatc gaaatcgtc 
      ttcgaattc gacaaagtg cgcgagttc accgccgtt 
      cacatctac accaacaac caatttaca aaggacgtg 
      caagtgttt tcgcaggcg aaagtgttg ttcagtgtg 
      ggagggaag cgctacaaa ggtgaaccc ataacattt 
      gattacatg gaagatcgc atctgggaa acgccacgc 
      aatgtgtcg attagactg taccatcgc gtggggcga 
      tttgtgaaa atccagcta caattcgcc gcgagatgg 
      ctgatgatt agcgaagtg actttcgat tcgtccgtc 
      gttaacggc aatttcacc gaggaggag gaggaggaa 
      ttggaggaa caaacactg caaaaagac caaatcgcg 
      tctccggga gccgacagc aagaacgtc gtgagcgcc 
      gtaaacacc ccgtctggg ctgggacaa tacctcggc 
      gtgatagtt ggcgtgtta tcagtgctg gtagtgtta 
      atggtagcg gtagtagtt ttcgttgta gtacaacaa 
      agaaggtat aaatcatct ccgcgaccg tctcaagtg 
      ccaggcggc tgcgataag gcagcactt tatcgtgaa 
      ccaagtatg ggaagactt ccgggtagt tctgattat 
      gcagacgtt cgagacccc gaatatgcc gtgccctta 
      cagcctcca caaactccc cgtgcgccg ccacccttg 
      cacaacttc ttcccgaaa ccgccgccg gttccgcca 
      ccccccgag cgctactat gcggccacg gaaatatgc 
      aactctggc tttgctcag cagacagcc gagatatgt 
      ctcgaatac gaggaccta cgttggacc ctaaactgt 
      gataaatcg actttacga tggggacga ctgcgttac 
      cattactta ctggctcca cgcccatca tttgccaag 
      gctgaagat taccaaata tgcttaact gaaatatct 
      tacgttgct tttacaata agacaatcc aatatcata 
      cgatgtgct atatgtgtt gtaaatagg gacccctat 
      tttaaatta tga
    mRNA sequence
    • >TC001549 RA
      cgagctttc ggacggaac atatggagg tttaggccc 
      aggaattag gtgggccag gtccaggac tcgctggtg 
      ggagacgct tcttggtgg gggcagata gggacctct 
      caccattgg atatccgag ttttcgctt tagtgttcc 
      gttccagcg tttgttgtg cagcggctc gaattgaat 
      ctttacgag tggagcttt acgcgggtt ttacgagtg 
      ccgttgctg gatatttta atctttatt atatgaatt 
      tcatgtgaa tttatggtt tttaactat tgcccaagg 
      catatggat tatatcatg tgaaggata agataaatg 
      tcgtaggct tcggtcgag ccggtctgc tgtaaaaat 
      gaaggtgct cctgtcgtc ggtgatgat cctgttggt 
      gacagcagc agatggcgt cgacccctc gcagtgcat 
      cgcgcctct gggcatgga atcgggcgc catcaagga 
      tcaggatat tacagccag ttcttcgtt cgagcatgg 
      caatgtggg accgcatca cggaaggtt gaggaacga 
      gttggatgg gggagcctg gtgtcctca aagcgccgc 
      cactcccga tttgaagga gtggataca aatagactt 
      acacacggt ccatatgat tacggcgac tgaaacgca 
      agggcggtt cgggaacgg gaaggggat ggagtttgc 
      ggaagccta tgtcctgga atactggcg acctcgctt 
      tttgaataa gtggattcg gtacaggaa ccgctcggg 
      tgaagaggc tctcaaagg caacatcaa tacatacct 
      cgagtcgaa gaccatcct ggacccgcc catatgggc 
      cagtaaaat caaatttct gcccttgag tcaccacga 
      aagaacggt ttgcatgcg cgtggaaat ctatgggtg 
      tcgctggac cgatggtat tcaaagtta ttcgatgcc 
      gcaaggtga taagcgtgg aactagttg ggagttcta 
      tgacacggc ctatgatgg gcactggga cggtgagaa 
      gctacaata cggattggg gcaattgac ggacgggaa 
      agtggcccc tgatgactt caaagcgtc gttttatca 
      aaccaattc gcagcaagg ctgggtcgg gtggaagaa 
      cgacacgcg taagaacaa accgatcga aatcgtctt 
      cgaattcga caaagtgcg cgagttcac cgccgttca 
      catctacac caacaacca atttacaaa ggacgtgca 
      agtgttttc gcaggcgaa agtgttgtt cagtgtggg 
      agggaagcg ctacaaagg tgaacccat aacatttga 
      ttacatgga agatcgcat ctgggaaac gccacgcaa 
      tgtgtcgat tagactgta ccatcgcgt ggggcgatt 
      tgtgaaaat ccagctaca attcgccgc gagatggct 
      gatgattag cgaagtgac tttcgattc gtccgtcgt 
      taacggcaa tttcaccga ggaggagga ggaggaatt 
      ggaggaaca aacactgca aaaagacca aatcgcgtc 
      tccgggagc cgacagcaa gaacgtcgt gagcgccgt 
      aaacacccc gtctgggct gggacaata cctcggcgt 
      gatagttgg cgtgttatc agtgctggt agtgttaat 
      ggtagcggt agtagtttt cgttgtagt acaacaaag 
      aaggtataa atcatctcc gcgaccgtc tcaagtgcc 
      aggcggctg cgataaggc agcacttta tcgtgaacc 
      aagtatggg aagacttcc gggtagttc tgattatgc 
      agacgttcg agaccccga atatgccgt gcccttaca 
      gcctccaca aactccccg tgcgccgcc acccttgca 
      caacttctt cccgaaacc gccgccggt tccgccacc 
      ccccgagcg ctactatgc ggccacgga aatatgcaa 
      ctctggctt tgctcagca gacagccga gatatgtct 
      cgaatacga ggacctacg ttggaccct aaactgtga 
      taaatcgac tttacgatg gggacgact gcgttacca 
      ttacttact ggctccacg cccatcatt tgccaaggc 
      tgaagatta ccaaatatg cttaactga aatatctta 
      cgttgcttt tacaataag acaatccaa tatcatacg 
      atgtgctat atgtgttgt aaataggga cccctattt 
      taaattatg aaattgttt atacctttg tacataatc 
      gtgatcaat gtaaataaa tagcgtata cgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY