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Gene information
General information
TC001529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3031193..3035387
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001529
    iBeetle screen iB_03202

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001529 RA
      MQSSRAPSV EDDEEGHLI YRSGDILQD RYKILGTLG 
      EGTFGKVVK VKDLEMDHS MALKIIKNV EKYREAAKL 
      EINVLEKLA DKDPDCIHL CVKMLDWFD YHGHMCIAF 
      EMLGLSVFD FLKDNNYQP YPLDQVRHI GYQLCYSVK 
      FLHDNKLTH TDLKPENIL FVDSDYDLV YNSKKRRDV 
      KRVKRTDVR LIDFGSATF DHEHHSTIV STRHYRAPE 
      VILELGWSQ PCDVWSIGC ILFELYLGI TLFQTHDNR 
      EHLAMMQRI LGEVPVRMA RKTKTKYFY RGKLEWDEK 
      SSAGRYVRD NCKPLMRYK QSDESDHNQ LFDLIFKML 
      DYEPSQRIT LKEAMLHPF FDKIAPHQR LGEHGAGDV 
      RRDKSLSMS R
    CDS sequence
    • >TC001529 RA
      atgcagagc tcacgcgcg ccctcagtg gaagacgat 
      gaagaaggc catctcatt taccgatcg ggagacatt 
      cttcaagac cgatataaa atacttggc actctcggc 
      gaaggcacc tttggcaag gtggttaaa gtgaaggac 
      cttgaaatg gaccactca atggcatta aaaattata 
      aaaaatgtg gaaaagtat cgagaggct gctaaatta 
      gaaattaac gttttggaa aaattggcc gataaagat 
      ccagactgt atacacttg tgtgtaaag atgttggac 
      tggttcgac tatcacggc catatgtgt atagctttt 
      gaaatgttg ggtctgagc gtgtttgac tttttgaaa 
      gacaacaat taccaaccg tatcctttg gatcaagtg 
      agacatatt ggatatcag ctgtgctat tctgttaaa 
      tttttacat gataataaa ctgacgcac actgatctc 
      aagccggag aatattcta ttcgtcgat tcggattac 
      gacttagtt tataatagt aaaaagagg agagacgtg 
      aaaagagta aaaagaact gatgttaga ttaatagac 
      tttgggagt gctacgttt gaccacgaa catcatagt 
      actatagtc tcgactcgt cattacaga gctcctgag 
      gttatttta gaattaggg tggtcacaa ccgtgcgat 
      gtttggtca atcggttgt attttattc gaattgtat 
      ctgggaatc accctcttc cagacacac gataatcgc 
      gaacactta gctatgatg caacgtatt cttggggag 
      gtgccagtt aggatggca cgcaagact aaaacaaaa 
      tacttctac cgtggtaag ttagagtgg gatgagaag 
      agttcggca ggtcgttat gtccgtgat aattgtaaa 
      ccattaatg aggtacaaa caaagtgac gaatcggac 
      cacaaccag ctcttcgat ttgattttt aaaatgttg 
      gattatgag ccgtctcag aggataacg ttgaaggag 
      gcaatgttg catcccttc tttgataaa atcgccccg 
      catcaacgt ttgggtgaa catggcgct ggtgacgtc 
      agaagagat aaaagcttg tcgatgtcg cgatga
    mRNA sequence
    • >TC001529 RA
      tgacggtgt tgacgaatg cagagctca cgcgcgccc 
      tcagtggaa gacgatgaa gaaggccat ctcatttac 
      cgatcggga gacattctt caagaccga tataaaata 
      cttggcact ctcggcgaa ggcaccttt ggcaaggtg 
      gttaaagtg aaggacctt gaaatggac cactcaatg 
      gcattaaaa attataaaa aatgtggaa aagtatcga 
      gaggctgct aaattagaa attaacgtt ttggaaaaa 
      ttggccgat aaagatcca gactgtata cacttgtgt 
      gtaaagatg ttggactgg ttcgactat cacggccat 
      atgtgtata gcttttgaa atgttgggt ctgagcgtg 
      tttgacttt ttgaaagac aacaattac caaccgtat 
      cctttggat caagtgaga catattgga tatcagctg 
      tgctattct gttaaattt ttacatgat aataaactg 
      acgcacact gatctcaag ccggagaat attctattc 
      gtcgattcg gattacgac ttagtttat aatagtaaa 
      aagaggaga gacgtgaaa agagtaaaa agaactgat 
      gttagatta atagacttt gggagtgct acgtttgac 
      cacgaacat catagtact atagtctcg actcgtcat 
      tacagagct cctgaggtt attttagaa ttagggtgg 
      tcacaaccg tgcgatgtt tggtcaatc ggttgtatt 
      ttattcgaa ttgtatctg ggaatcacc ctcttccag 
      acacacgat aatcgcgaa cacttagct atgatgcaa 
      cgtattctt ggggaggtg ccagttagg atggcacgc 
      aagactaaa acaaaatac ttctaccgt ggtaagtta 
      gagtgggat gagaagagt tcggcaggt cgttatgtc 
      cgtgataat tgtaaacca ttaatgagg tacaaacaa 
      agtgacgaa tcggaccac aaccagctc ttcgatttg 
      atttttaaa atgttggat tatgagccg tctcagagg 
      ataacgttg aaggaggca atgttgcat cccttcttt 
      gataaaatc gccccgcat caacgtttg ggtgaacat 
      ggcgctggt gacgtcaga agagataaa agcttgtcg 
      atgtcgcga tgagagaga ggtctcgtg tgtttttac 
      acctatttc atcattttg caacagtca tgtatttga 
      gtatcggac aataaaaaa gctgttttt attctgatt 
      tgtacaatt tgggtttgt gacatgttt acgatgtgt 
      atatgagtc gctatctga tttcaactt gttctattt 
      tctacccat cttaagtcc ataggtttt atttataag 
      ggtaattta atgaatgtt acgagttac gtgtacata 
      ttaaataat aaagattta atttttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY