Status
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Gene information
General information
TC031939
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001527,TC001526
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 607152..616692
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031939
    iBeetle screen iB_03201
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031939 RA
      MPRSRRRHH SRSRSRSRS HSHDRDREP KRRKVDSLD 
      SPNHKRSVS CEPEIPSTT VEERETMEI SNNDVTIDE 
      SNESTTEIS KTDESQDNL DDSSTNTPQ KEKTRKEEK 
      LLITPKKTM SPKQIESEK KRQQKRLER EEREKKKQE 
      ERERVRLEK EKQKQEKEK QKQEKEELK KKKNEEKAK 
      ERELKEEQK RKEKEEKEA KRKEKQERE ETKRKEREQ 
      DKLRKQQEI EEKNREKQK LEEQKQKTA QAFVNFFVP 
      KKIETKVDE PKTRLFMPF QVKSDMRLA PLTRHVLSV 
      EGKNNLDES LVKNDLDVL YLSELKQGR KTGTCGRTW 
      PFEEDVIIV EEDFGESIC EDKQKKKMR AKFLKFHEN 
      RRPAYYGTW RKKSCVVKP RRPFGEDRI FDYEVDSDD 
      DWEEEEQGE SLNGSDDED KENEAENED YEVDNEFFV 
      PHGHLSDDE VDDEATAKL SPESHKQKL KLLKDEFDQ 
      DMQTKTNKI KPRSIGCIW YKRDGSNVD EAIDRHLRP 
      LGIISNGQI VIKKRKDLV GLTPNRKYA KELREDLIP 
      AFLKLIQGN VNKRKMIVD EFISYMQNN GYTVEVSKT 
      YLGKRLKQF AQWKKCLEE GPMLNKFCW CLEDEVKKR 
      YLE
    • >TC031939 RB
      MFRFMRQLE DLNRFGVPS YQMPRSRRR HHSRSRSRS 
      RSHSHDRDR EPKRRKVDS LDSPNHKRS VSCEPEIPS 
      TTVEERETM EISNNDVTI DESNESTTE ISKTDESQD 
      NLDDSSTNT PQKEKTRKE EKLLITPKK TMSPKQIES 
      EKKRQQKRL EREEREKKK QEERERVRL EKEKQKQEK 
      EKQKQEKEE LKKKKNEEK AKERELKEE QKRKEKEEK 
      EAKRKEKQE REETKRKER EQDKLRKQQ EIEEKNREK 
      QKLEEQKQK TAQAFVNFF VPKKIETKV DEPKTRLFM 
      PFQVKSDMR LAPLTRHVL SVEGKNNLD ESLVKNDLD 
      VLYLSELKQ GRKTGTCGR TWPFEEDVI IVEEDFGES 
      ICEDKQKKK MRAKFLKFH ENRRPAYYG TWRKKSCVV 
      KPRRPFGED RIFDYEVDS DDDWEEEEQ GESLNGSDD 
      EDKENEAEN EDYEVDNEF FVPHGHLSD DEVDDEATA 
      KLSPESHKQ KLKLLKDEF DQDMQTKTN KIKPRSIGC 
      IWYKRDGSN VDEAIDRHL RPLGIISNG QIVIKKRKD 
      LVGLTPNRK YAKELREDL IPAFLKLIQ GNVNKRKMI 
      VDEFISYMQ NNGYTVEVS KTYLGKRLK QFAQWKKCL 
      EEGPMLNKF CWCLEDEVK KRYLE
    CDS sequence
    • >TC031939 RA
      atgccccgc tcgaggcga agacaccac tcccggtcc 
      agatccagg tcccggtcg cattcccac gacagagat 
      cgggagcct aagcggaga aaagtcgac agtcttgat 
      agccccaac cacaagaga tccgtgagt tgtgaacct 
      gaaattcct tcaacaaca gttgaagaa cgtgaaaca 
      atggaaatt tcaaataat gacgtcaca atcgacgaa 
      tcaaacgaa agcacaact gaaatttcc aaaaccgat 
      gaatctcaa gataatttg gacgacagc tcaaccaat 
      acccctcaa aaagaaaag acccgaaaa gaggaaaaa 
      ctgctcata acacccaaa aaaacaatg agcccgaaa 
      cagatcgaa tcggagaag aaacgccaa cagaagcgc 
      ctcgagcgt gaagaacgc gaaaaaaag aaacaagaa 
      gaacgtgaa cgcgtccgc cttgaaaaa gaaaaacaa 
      aaacaagag aaagagaaa caaaaacag gaaaaagaa 
      gaactcaaa aagaagaaa aacgaagaa aaagccaaa 
      gagcgcgaa ctaaaagaa gaacaaaaa cgcaaagaa 
      aaggaggag aaagaagcg aaacgtaag gaaaaacaa 
      gagcgtgaa gaaaccaaa cgtaaagaa cgcgaacaa 
      gacaaactg cgcaaacag caagaaatc gaggagaaa 
      aatcgcgaa aaacaaaaa ctcgaagaa caaaaacag 
      aaaacggcc caagcgttt gtcaacttt ttcgttccg 
      aagaaaatc gaaacgaaa gttgatgaa cccaaaact 
      aggcttttt atgccgttt caggtgaaa tccgatatg 
      aggttggca ccactaacg cgacacgtg ttgtcggtt 
      gaagggaaa aataatctt gatgagagt ttggtgaag 
      aatgacttg gacgtgtta tatttgagt gaattgaaa 
      caagggagg aagactggg acgtgtggg agaacgtgg 
      ccctttgag gaggatgtg ataatcgtt gaggaagat 
      tttggtgag agtatttgc gaagataag cagaagaag 
      aagatgagg gccaagttt ttaaagttt catgagaat 
      cgaaggccg gcttattac ggtacgtgg aggaagaag 
      agttgcgtt gttaagcca cgaagaccg tttggtgaa 
      gataggatt tttgattat gaggttgat tcggatgat 
      gactgggag gaagaagag caaggggag tcactgaat 
      gggtctgat gatgaagat aaggagaat gaggctgag 
      aatgaagac tatgaggtt gataatgag ttctttgtg 
      cctcatggt catctcagt gatgatgag gttgatgat 
      gaggcgacg gcgaagttg tcaccggag tcgcataag 
      caaaagttg aagttgctg aaagacgag ttcgatcag 
      gatatgcaa actaagact aacaaaatt aaaccgcgg 
      tcgattggc tgtatttgg tacaagaga gatgggtct 
      aatgttgat gaggcgatc gatagacat ttgagaccg 
      ttggggatc atcagcaat ggtcagatt gttatcaag 
      aagaggaag gatctggtt ggcttgact ccaaatcgg 
      aaatacgcc aaggaatta cgagaggat ttaattccg 
      gcctttttg aaactcatc caagggaat gtgaacaag 
      agaaagatg attgttgat gagtttatt agctatatg 
      caaaataat gggtacaca gttgaagtg tctaaaacc 
      tatctcggg aagaggttg aagcagttc gctcagtgg 
      aagaagtgc cttgaggag ggtccaatg ttgaataag 
      ttttgttgg tgtcttgaa gacgaagtt aagaagaga 
      tatttggag tag
    • >TC031939 RB
      atgttccga tttatgcgc cagttggaa gacttgaac 
      cgtttcggg gtgccgtcc taccaaatg ccccgctcg 
      aggcgaaga caccactcc cggtccaga tccaggtcc 
      cggtcgcat tcccacgac agagatcgg gagcctaag 
      cggagaaaa gtcgacagt cttgatagc cccaaccac 
      aagagatcc gtgagttgt gaacctgaa attccttca 
      acaacagtt gaagaacgt gaaacaatg gaaatttca 
      aataatgac gtcacaatc gacgaatca aacgaaagc 
      acaactgaa atttccaaa accgatgaa tctcaagat 
      aatttggac gacagctca accaatacc cctcaaaaa 
      gaaaagacc cgaaaagag gaaaaactg ctcataaca 
      cccaaaaaa acaatgagc ccgaaacag atcgaatcg 
      gagaagaaa cgccaacag aagcgcctc gagcgtgaa 
      gaacgcgaa aaaaagaaa caagaagaa cgtgaacgc 
      gtccgcctt gaaaaagaa aaacaaaaa caagagaaa 
      gagaaacaa aaacaggaa aaagaagaa ctcaaaaag 
      aagaaaaac gaagaaaaa gccaaagag cgcgaacta 
      aaagaagaa caaaaacgc aaagaaaag gaggagaaa 
      gaagcgaaa cgtaaggaa aaacaagag cgtgaagaa 
      accaaacgt aaagaacgc gaacaagac aaactgcgc 
      aaacagcaa gaaatcgag gagaaaaat cgcgaaaaa 
      caaaaactc gaagaacaa aaacagaaa acggcccaa 
      gcgtttgtc aactttttc gttccgaag aaaatcgaa 
      acgaaagtt gatgaaccc aaaactagg ctttttatg 
      ccgtttcag gtgaaatcc gatatgagg ttggcacca 
      ctaacgcga cacgtgttg tcggttgaa gggaaaaat 
      aatcttgat gagagtttg gtgaagaat gacttggac 
      gtgttatat ttgagtgaa ttgaaacaa gggaggaag 
      actgggacg tgtgggaga acgtggccc tttgaggag 
      gatgtgata atcgttgag gaagatttt ggtgagagt 
      atttgcgaa gataagcag aagaagaag atgagggcc 
      aagttttta aagtttcat gagaatcga aggccggct 
      tattacggt acgtggagg aagaagagt tgcgttgtt 
      aagccacga agaccgttt ggtgaagat aggattttt 
      gattatgag gttgattcg gatgatgac tgggaggaa 
      gaagagcaa ggggagtca ctgaatggg tctgatgat 
      gaagataag gagaatgag gctgagaat gaagactat 
      gaggttgat aatgagttc tttgtgcct catggtcat 
      ctcagtgat gatgaggtt gatgatgag gcgacggcg 
      aagttgtca ccggagtcg cataagcaa aagttgaag 
      ttgctgaaa gacgagttc gatcaggat atgcaaact 
      aagactaac aaaattaaa ccgcggtcg attggctgt 
      atttggtac aagagagat gggtctaat gttgatgag 
      gcgatcgat agacatttg agaccgttg gggatcatc 
      agcaatggt cagattgtt atcaagaag aggaaggat 
      ctggttggc ttgactcca aatcggaaa tacgccaag 
      gaattacga gaggattta attccggcc tttttgaaa 
      ctcatccaa gggaatgtg aacaagaga aagatgatt 
      gttgatgag tttattagc tatatgcaa aataatggg 
      tacacagtt gaagtgtct aaaacctat ctcgggaag 
      aggttgaag cagttcgct cagtggaag aagtgcctt 
      gaggagggt ccaatgttg aataagttt tgttggtgt 
      cttgaagac gaagttaag aagagatat ttggagtag
    mRNA sequence
    • >TC031939 RA
      agttagttt tcttcctcc ttgatggtt gcttcatcg 
      tgaatgttg aacgtccaa gttgtgtgt gttttgcca 
      aggttttgc cgaaaattc acgctaaag cccttaatg 
      tgagccccc aagattatc aagcgtgtc ccaaggcca 
      agtttgtgt ggtttcgtg aagtttttc tttgatgaa 
      ttcaaaata aaaagagca agtcaacga caccactct 
      ccaaccaat aggttggat cgatgtttg gcttctcct 
      tggccacac agctatttc aaggttcgg cccaacgta 
      cttggaatt gtgttaccc aattcacga atttaattt 
      ggtacaaat tcaaccaca tacggcatc caatcatgc 
      cccgctcga ggcgaagac accactccc ggtccagat 
      ccaggtccc ggtcgcatt cccacgaca gagatcggg 
      agcctaagc ggagaaaag tcgacagtc ttgatagcc 
      ccaaccaca agagatccg tgagttgtg aacctgaaa 
      ttccttcaa caacagttg aagaacgtg aaacaatgg 
      aaatttcaa ataatgacg tcacaatcg acgaatcaa 
      acgaaagca caactgaaa tttccaaaa ccgatgaat 
      ctcaagata atttggacg acagctcaa ccaataccc 
      ctcaaaaag aaaagaccc gaaaagagg aaaaactgc 
      tcataacac ccaaaaaaa caatgagcc cgaaacaga 
      tcgaatcgg agaagaaac gccaacaga agcgcctcg 
      agcgtgaag aacgcgaaa aaaagaaac aagaagaac 
      gtgaacgcg tccgccttg aaaaagaaa aacaaaaac 
      aagagaaag agaaacaaa aacaggaaa aagaagaac 
      tcaaaaaga agaaaaacg aagaaaaag ccaaagagc 
      gcgaactaa aagaagaac aaaaacgca aagaaaagg 
      aggagaaag aagcgaaac gtaaggaaa aacaagagc 
      gtgaagaaa ccaaacgta aagaacgcg aacaagaca 
      aactgcgca aacagcaag aaatcgagg agaaaaatc 
      gcgaaaaac aaaaactcg aagaacaaa aacagaaaa 
      cggcccaag cgtttgtca actttttcg ttccgaaga 
      aaatcgaaa cgaaagttg atgaaccca aaactaggc 
      tttttatgc cgtttcagg tgaaatccg atatgaggt 
      tggcaccac taacgcgac acgtgttgt cggttgaag 
      ggaaaaata atcttgatg agagtttgg tgaagaatg 
      acttggacg tgttatatt tgagtgaat tgaaacaag 
      ggaggaaga ctgggacgt gtgggagaa cgtggccct 
      ttgaggagg atgtgataa tcgttgagg aagattttg 
      gtgagagta tttgcgaag ataagcaga agaagaaga 
      tgagggcca agtttttaa agtttcatg agaatcgaa 
      ggccggctt attacggta cgtggagga agaagagtt 
      gcgttgtta agccacgaa gaccgtttg gtgaagata 
      ggatttttg attatgagg ttgattcgg atgatgact 
      gggaggaag aagagcaag gggagtcac tgaatgggt 
      ctgatgatg aagataagg agaatgagg ctgagaatg 
      aagactatg aggttgata atgagttct ttgtgcctc 
      atggtcatc tcagtgatg atgaggttg atgatgagg 
      cgacggcga agttgtcac cggagtcgc ataagcaaa 
      agttgaagt tgctgaaag acgagttcg atcaggata 
      tgcaaacta agactaaca aaattaaac cgcggtcga 
      ttggctgta tttggtaca agagagatg ggtctaatg 
      ttgatgagg cgatcgata gacatttga gaccgttgg 
      ggatcatca gcaatggtc agattgtta tcaagaaga 
      ggaaggatc tggttggct tgactccaa atcggaaat 
      acgccaagg aattacgag aggatttaa ttccggcct 
      ttttgaaac tcatccaag ggaatgtga acaagagaa 
      agatgattg ttgatgagt ttattagct atatgcaaa 
      ataatgggt acacagttg aagtgtcta aaacctatc 
      tcgggaaga ggttgaagc agttcgctc agtggaaga 
      agtgccttg aggagggtc caatgttga ataagtttt 
      gttggtgtc ttgaagacg aagttaaga agagatatt 
      tggagtagt tttaagcat aattttgta ttccgttgt 
      ccaaataaa cgtttttag taaat
    • >TC031939 RB
      agttagttt tcttcctcc ttgatggtt gcttcatcg 
      tgaatgttg aacgtccaa gttgtgtgt gttttgcca 
      aggttttgc cgaaaattc acgctaaag cccttaatg 
      tgagccccc aagattatc aagcgtgtc ccaaggcca 
      agtttgtgt ggtttcgtg aagtttttc tttgatgaa 
      ttcaaaata aaaagagca agtcaacga caccactct 
      ccaaccaat aggttggat cgatgtttg gcttctcct 
      tggccacac agctatttc aaggttcgg cccaacgta 
      cttggaatt gtgttaccc aattcacga atttaattt 
      ggtacaaat tcaaccaca tacggcatc caatcgtaa 
      tgccttcat ttttgcaca atctcccta atcgagtag 
      gcttaactg tcaaaaaaa ccaccagaa acaaaagat 
      tttttcaac ttgttcgag ttcaatatt tccgtttca 
      tcgccattt caaattaat tttgcaaca aaaccacaa 
      aaatgttcc gatttatgc gccagttgg aagacttga 
      accgtttcg gggtgccgt cctaccaaa tgccccgct 
      cgaggcgaa gacaccact cccggtcca gatccaggt 
      cccggtcgc attcccacg acagagatc gggagccta 
      agcggagaa aagtcgaca gtcttgata gccccaacc 
      acaagagat ccgtgagtt gtgaacctg aaattcctt 
      caacaacag ttgaagaac gtgaaacaa tggaaattt 
      caaataatg acgtcacaa tcgacgaat caaacgaaa 
      gcacaactg aaatttcca aaaccgatg aatctcaag 
      ataatttgg acgacagct caaccaata cccctcaaa 
      aagaaaaga cccgaaaag aggaaaaac tgctcataa 
      cacccaaaa aaacaatga gcccgaaac agatcgaat 
      cggagaaga aacgccaac agaagcgcc tcgagcgtg 
      aagaacgcg aaaaaaaga aacaagaag aacgtgaac 
      gcgtccgcc ttgaaaaag aaaaacaaa aacaagaga 
      aagagaaac aaaaacagg aaaaagaag aactcaaaa 
      agaagaaaa acgaagaaa aagccaaag agcgcgaac 
      taaaagaag aacaaaaac gcaaagaaa aggaggaga 
      aagaagcga aacgtaagg aaaaacaag agcgtgaag 
      aaaccaaac gtaaagaac gcgaacaag acaaactgc 
      gcaaacagc aagaaatcg aggagaaaa atcgcgaaa 
      aacaaaaac tcgaagaac aaaaacaga aaacggccc 
      aagcgtttg tcaactttt tcgttccga agaaaatcg 
      aaacgaaag ttgatgaac ccaaaacta ggcttttta 
      tgccgtttc aggtgaaat ccgatatga ggttggcac 
      cactaacgc gacacgtgt tgtcggttg aagggaaaa 
      ataatcttg atgagagtt tggtgaaga atgacttgg 
      acgtgttat atttgagtg aattgaaac aagggagga 
      agactggga cgtgtggga gaacgtggc cctttgagg 
      aggatgtga taatcgttg aggaagatt ttggtgaga 
      gtatttgcg aagataagc agaagaaga agatgaggg 
      ccaagtttt taaagtttc atgagaatc gaaggccgg 
      cttattacg gtacgtgga ggaagaaga gttgcgttg 
      ttaagccac gaagaccgt ttggtgaag ataggattt 
      ttgattatg aggttgatt cggatgatg actgggagg 
      aagaagagc aaggggagt cactgaatg ggtctgatg 
      atgaagata aggagaatg aggctgaga atgaagact 
      atgaggttg ataatgagt tctttgtgc ctcatggtc 
      atctcagtg atgatgagg ttgatgatg aggcgacgg 
      cgaagttgt caccggagt cgcataagc aaaagttga 
      agttgctga aagacgagt tcgatcagg atatgcaaa 
      ctaagacta acaaaatta aaccgcggt cgattggct 
      gtatttggt acaagagag atgggtcta atgttgatg 
      aggcgatcg atagacatt tgagaccgt tggggatca 
      tcagcaatg gtcagattg ttatcaaga agaggaagg 
      atctggttg gcttgactc caaatcgga aatacgcca 
      aggaattac gagaggatt taattccgg cctttttga 
      aactcatcc aagggaatg tgaacaaga gaaagatga 
      ttgttgatg agtttatta gctatatgc aaaataatg 
      ggtacacag ttgaagtgt ctaaaacct atctcggga 
      agaggttga agcagttcg ctcagtgga agaagtgcc 
      ttgaggagg gtccaatgt tgaataagt tttgttggt 
      gtcttgaag acgaagtta agaagagat atttggagt 
      agttttaag cataatttt gtattccgt tgtccaaat 
      aaacgtttt tagtaaat