Status
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Gene information
General information
TC001512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2454263..2516083
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001512
    iBeetle screen iB_03200

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001512 RA
      MLSEFVYLF PLLFLLPRT KSDNLPFRP ISKPLTFPK 
      ENRLNEFIR HYEPLSYDT SEVHRAHNR ARRSVSRDN 
      LVHVSFRAH NRDFHLRLK RDLDVFSDS MEVHGPEGR 
      IDVDTSHVY KGHLLGEPE SSVFGSLID GVFEGKIVS 
      PKGSFYVEK ARHYFPHTT HPNRTFHSV IYHEDHVED 
      PYEHVREGH SAGCGITDE VIQWMDRVQ NSGVDDEPP 
      PPPPKETFR KRKKATNGD KVQFKSVEN HEDQISKYS 
      KEANEDHAR HKRATRRDE NKNTCSLYI QTDPLIWRH 
      IREGFPEHS DPRKENEVN EKTREEILS LIAHHVTAV 
      NYIYRDTKF DGRSEHRNI KFEVQRIKI DDYSSCSCK 
      RCPEMNQFC LENIDVSNF LNIHSLGNH EDFCLAYVF 
      TYRDFTGGT LGLAWVASA SGASGGICE RYKTYTETI 
      GGMYQSTKR SLNTGIITF VNYNSRVPP KVSQLTLAH 
      EIGHNFGSP HDYPPECRP GGLNGNFIM FASATSGDR 
      PNNSKFSSC SVGNISNVL DAIEDNKKR NCFTASAGA 
      FCGNKIVEA GEECDCGYD DNECQDKCC YPRLVSTQD 
      KQENASAKG CQRRRGTQC SPSQGPCCS GDTCRFVPA 
      YYGEVCKTE SDCSMSSKC DGKSAECPQ PQPRPDKTR 
      CNNGTQLCI KGECTGSIC LEWNLQACS ITSQGNPNI 
      DKRKLCELA CQNGTDMCR STSEFAEKV GLPPGGISL 
      RPGAPCDNF QGYCDVFLK CRAVDADGP LVRLMNLLL 
      NKETLLSVA QWITNYWWA VLLMGIAFI IFMGLFIKC 
      CAVHTPSSN PKKRPARRI SDTLRRPMN TLRRMRHHQ 
      NGAQHAAPH GAVNSRNRS ERSTRPSAS QGPRPGYGE 
      GRGHYHAPK ATAPPSGFN RQNHPYAGA YDRNAYEMR 
      HKV
    CDS sequence
    • >TC001512 RA
      atgttgagt gaatttgtt tacttgttc cctttgctt 
      ttcttgctt cctcgaacc aaaagtgat aatttgccc 
      ttcagaccc atcagtaaa ccgctgaca tttccaaaa 
      gaaaaccgc ctcaacgag ttcatccgc cactacgag 
      cctctatcg tacgacacc tccgaagtg catcgagcc 
      cacaaccgt gctcgtcgt tctgtcagt cgcgataat 
      ctcgtccac gtgtcgttc cgagctcac aaccgtgat 
      ttccatcta agactgaaa agagacctt gatgtgttc 
      agcgattcg atggaggtt cacggtccc gaaggccgg 
      atagacgtc gatacgtcg cacgtctac aaaggccac 
      cttttaggt gagcccgag agttcggtc tttggctcg 
      ttaattgat ggagtcttt gaagggaag atcgtatca 
      cctaaaggc tctttttac gttgagaag gctcgtcat 
      tattttccc cataccaca catccaaac agaacattc 
      cattcggtc atttaccac gaggatcac gtagaggat 
      ccatacgaa catgttaga gagggtcat tcagctgga 
      tgcggaatc accgacgag gtgatccag tggatggac 
      cgcgtccag aattcgggg gtggacgac gagccgccg 
      ccgccgccg cccaaagaa acattccga aaacgtaaa 
      aaagccacc aatggtgat aaagttcaa ttcaagtcc 
      gtcgaaaat cacgaagac cagatcagc aagtattcg 
      aaagaggcg aatgaagat cacgcaaga cataaaagg 
      gcaacacga agggacgaa aacaagaac acttgttcg 
      ttgtatatc cagacggat ccactcatc tggagacac 
      atcagggag ggctttcca gagcactct gacccgagg 
      aaggaaaac gaagtcaac gaaaagaca cgcgaagaa 
      atcctgtca ttaatcgca catcacgtc actgccgtt 
      aattatatt tatcgtgat acgaaattt gatgggaga 
      agcgaacat cgcaatatc aaatttgaa gttcaaagg 
      attaagatc gacgactat tcctcctgc agctgcaag 
      cgatgcccc gaaatgaac cagttttgt ttagaaaat 
      atcgacgtt agtaatttc ttgaatatc cattcgttg 
      ggcaatcac gaggacttt tgtctggct tatgttttc 
      acgtatcgg gattttacg ggtggtact ctggggcta 
      gcttgggtc gctagtgcg tccggggct tccggcggt 
      atctgcgag agatacaag acttacacg gaaacaata 
      ggaggcatg tatcagtcg acgaagcgc tccttaaac 
      acagggatc atcactttc gtcaactac aacagcagg 
      gtgcctcct aaggtctcc cagctcaca ctagcgcac 
      gaaatcgga cacaatttc ggctctccc cacgactat 
      ccgcccgaa tgccgtccg ggtggcctt aacggcaac 
      ttcataatg ttcgcgtcg gcaacaagc ggcgaccgc 
      cccaacaac agcaaattt tcaagttgt agcgtcggc 
      aacatctcc aatgtgctc gacgccatc gaagacaat 
      aaaaagcgc aattgtttc accgcatcg gctggtgct 
      ttctgtggc aataaaatc gtcgaagcc ggcgaagag 
      tgtgattgt ggttacgac gacaacgag tgtcaagat 
      aagtgttgc taccctcgt cttgtcagc actcaagac 
      aagcaagaa aacgcttca gcgaaaggc tgtcagagg 
      cgaagaggc acccaatgc agtcctagt caaggcccg 
      tgctgcagt ggcgacacc tgccgcttc gtcccagcc 
      tactacggc gaggtttgc aaaaccgag tcggattgt 
      tcgatgtcg tcgaaatgc gacgggaaa tcggctgag 
      tgcccacag ccccaacca cggccggat aagactagg 
      tgcaataat gggacacag ttgtgtatt aagggggag 
      tgcacgggg tccatttgt ctggaatgg aacctacaa 
      gcttgttcg attacctcg caaggtaat ccaaacatc 
      gataagaga aaactgtgc gagttggct tgtcagaac 
      ggaacggac atgtgtcga agtacgagc gaattcgct 
      gagaaagtc gggcttcca ccaggtggg atcagccta 
      aggccggga gcgccgtgc gataatttt cagggttac 
      tgtgacgta ttcctcaaa tgccgagcc gtcgacgct 
      gacggcccc ctagtcaga ttgatgaat cttctattg 
      aacaaagag acgctgctc agcgtcgcc caatggata 
      acgaattac tggtgggcg gtgctgttg atgggcatc 
      gcgtttatc attttcatg ggtttgttt atcaaatgt 
      tgcgccgta catacgccg tcttccaat ccgaagaaa 
      cggccggcg agacgtatc agcgatacg ctccgcaga 
      cccatgaac acgctccgg cgaatgcga caccaccaa 
      aacggggcg caacatgcg gcgccccat ggggctgta 
      aatagtcga aatcgcagt gaaaggtcc acgagaccg 
      agcgctagt caagggccc aggccgggc tacggggag 
      ggaagggga cactatcat gcgccgaaa gcgacagcg 
      ccgccttcc ggtttcaac cgccaaaac cacccatac 
      gcaggggcg tacgaccgt aacgcctac gagatgcgt 
      cacaaagtt tga
    mRNA sequence
    • >TC001512 RA
      aatgaatcg gttttacag taaactggt ttttattaa 
      attccaagt ggccaaaga cttgtcgtt tcagtttcg 
      acttggtgg cccccttgt tgacatgtc tcagtcacc 
      catcaataa ttctcgtta atttggtca aaatttgta 
      aaataaaac agcaacgca tgcgcagtg gcgtctaaa 
      gacaaaggt gttgtgtac aaaccagtg catcagtca 
      gctgtaaat tcaggaatg gcttagttg gggtgtgtt 
      tttgtggtg tttgtggga ttattttgt gcaggaaaa 
      tgttgagtg aatttgttt acttgttcc ctttgcttt 
      tcttgcttc ctcgaacca aaagtgata atttgccct 
      tcagaccca tcagtaaac cgctgacat ttccaaaag 
      aaaaccgcc tcaacgagt tcatccgcc actacgagc 
      ctctatcgt acgacacct ccgaagtgc atcgagccc 
      acaaccgtg ctcgtcgtt ctgtcagtc gcgataatc 
      tcgtccacg tgtcgttcc gagctcaca accgtgatt 
      tccatctaa gactgaaaa gagaccttg atgtgttca 
      gcgattcga tggaggttc acggtcccg aaggccgga 
      tagacgtcg atacgtcgc acgtctaca aaggccacc 
      ttttaggtg agcccgaga gttcggtct ttggctcgt 
      taattgatg gagtctttg aagggaaga tcgtatcac 
      ctaaaggct ctttttacg ttgagaagg ctcgtcatt 
      attttcccc ataccacac atccaaaca gaacattcc 
      attcggtca tttaccacg aggatcacg tagaggatc 
      catacgaac atgttagag agggtcatt cagctggat 
      gcggaatca ccgacgagg tgatccagt ggatggacc 
      gcgtccaga attcggggg tggacgacg agccgccgc 
      cgccgccgc ccaaagaaa cattccgaa aacgtaaaa 
      aagccacca atggtgata aagttcaat tcaagtccg 
      tcgaaaatc acgaagacc agatcagca agtattcga 
      aagaggcga atgaagatc acgcaagac ataaaaggg 
      caacacgaa gggacgaaa acaagaaca cttgttcgt 
      tgtatatcc agacggatc cactcatct ggagacaca 
      tcagggagg gctttccag agcactctg acccgagga 
      aggaaaacg aagtcaacg aaaagacac gcgaagaaa 
      tcctgtcat taatcgcac atcacgtca ctgccgtta 
      attatattt atcgtgata cgaaatttg atgggagaa 
      gcgaacatc gcaatatca aatttgaag ttcaaagga 
      ttaagatcg acgactatt cctcctgca gctgcaagc 
      gatgccccg aaatgaacc agttttgtt tagaaaata 
      tcgacgtta gtaatttct tgaatatcc attcgttgg 
      gcaatcacg aggactttt gtctggctt atgttttca 
      cgtatcggg attttacgg gtggtactc tggggctag 
      cttgggtcg ctagtgcgt ccggggctt ccggcggta 
      tctgcgaga gatacaaga cttacacgg aaacaatag 
      gaggcatgt atcagtcga cgaagcgct ccttaaaca 
      cagggatca tcactttcg tcaactaca acagcaggg 
      tgcctccta aggtctccc agctcacac tagcgcacg 
      aaatcggac acaatttcg gctctcccc acgactatc 
      cgcccgaat gccgtccgg gtggcctta acggcaact 
      tcataatgt tcgcgtcgg caacaagcg gcgaccgcc 
      ccaacaaca gcaaatttt caagttgta gcgtcggca 
      acatctcca atgtgctcg acgccatcg aagacaata 
      aaaagcgca attgtttca ccgcatcgg ctggtgctt 
      tctgtggca ataaaatcg tcgaagccg gcgaagagt 
      gtgattgtg gttacgacg acaacgagt gtcaagata 
      agtgttgct accctcgtc ttgtcagca ctcaagaca 
      agcaagaaa acgcttcag cgaaaggct gtcagaggc 
      gaagaggca cccaatgca gtcctagtc aaggcccgt 
      gctgcagtg gcgacacct gccgcttcg tcccagcct 
      actacggcg aggtttgca aaaccgagt cggattgtt 
      cgatgtcgt cgaaatgcg acgggaaat cggctgagt 
      gcccacagc cccaaccac ggccggata agactaggt 
      gcaataatg ggacacagt tgtgtatta agggggagt 
      gcacggggt ccatttgtc tggaatgga acctacaag 
      cttgttcga ttacctcgc aaggtaatc caaacatcg 
      ataagagaa aactgtgcg agttggctt gtcagaacg 
      gaacggaca tgtgtcgaa gtacgagcg aattcgctg 
      agaaagtcg ggcttccac caggtggga tcagcctaa 
      ggccgggag cgccgtgcg ataattttc agggttact 
      gtgacgtat tcctcaaat gccgagccg tcgacgctg 
      acggccccc tagtcagat tgatgaatc ttctattga 
      acaaagaga cgctgctca gcgtcgccc aatggataa 
      cgaattact ggtgggcgg tgctgttga tgggcatcg 
      cgtttatca ttttcatgg gtttgttta tcaaatgtt 
      gcgccgtac atacgccgt cttccaatc cgaagaaac 
      ggccggcga gacgtatca gcgatacgc tccgcagac 
      ccatgaaca cgctccggc gaatgcgac accaccaaa 
      acggggcgc aacatgcgg cgccccatg gggctgtaa 
      atagtcgaa atcgcagtg aaaggtcca cgagaccga 
      gcgctagtc aagggccca ggccgggct acggggagg 
      gaaggggac actatcatg cgccgaaag cgacagcgc 
      cgccttccg gtttcaacc gccaaaacc acccatacg 
      caggggcgt acgaccgta acgcctacg agatgcgtc 
      acaaagttt gacagaacg gtcaagtcg gggcaattc 
      gggtgtctc cgccgcccg attaccccg gcacaccaa 
      tggccaaca tacatttcg agggtccca tggcacaat 
      cgtcaaacc agcaaaaga acactagtg tacagagtg 
      gctcaactt aacaaactc gaaacgatt gttgtgctc 
      tgtgatcga ttcgaacca cgaaccaat aaaaagtgc 
      gtgtgatat tacgaaact cgaaatgat tgattgaaa 
      acgggtgca tttatgtat agtcaccta ggttttagg 
      gatgagttt ttatgatat tagttggct tccgacgaa 
      aggctgaac ggatttgcg gttttatct gccaactcg 
      attttttaa tttatttat ttgccattt ctgcaccaa 
      atgattagg ttttaggtt cacgtacat atccgaaca 
      aatttgtcg atcaaagtt tttgattat gggggaatt 
      gaattggat tttgattgt ggattcggt ttgtccata 
      attccaagt ggttacatc acagctgcg ttttttagt 
      aaaattgtg gaaatttcg tgcagagat ctcattttg 
      aatgatttg tcctgaaag ttgctattt ttatataat 
      tgagggacg caattttac atttggtac attgttgaa 
      tttcaggaa attgtgata caaatctaa tttgctgtt 
      ttgtaatgt ctcaatgga gaaatctat atttgatgc 
      tgaaaatct gtattaatg aattgtaaa atattgata 
      tattatgta aatattgga aaaacatag ttgttttta 
      tatgaagta tgtatcata ttcttcact tttgtttta 
      gagtagtgg attttacct atgtctagc atttgtttg 
      attttttga cgacgtaaa agtaattgt atcaaattt 
      ttttattat gctagtagg tacaatcca acctcgatg 
      accaataat tgttgtgca tgtcgctta atacatgta 
      ccaaagtca ttggatgac tttttataa tactgtgaa 
      taataaatg tgttattgt caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY