Status
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Gene information
General information
TC001511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2445427..2454013
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001511
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001511 RA
      MFSAIKRFA SKGDGSPSN STVVGRPPS HQAMSTSLQ 
      RKFARGVQY NMKIVIKGD RNVGKTCLF HRLQGKKFI 
      EEYIPTEEI QVTSIQWNY KATDDIVKV EVWDVVDRG 
      KKKKRFEGL KLENSQIEM PEEPALDAE FLDVYKGTN 
      GVIMMLDLT KTWTFDYVQ RELPKVPAH IPVIILANH 
      CDMGHHRTV TSDHVTFYI ESIASTRSA QIRYSESSM 
      RNGFGLKLL HKFFNLPFL QLQKETLLR QLERNEMEI 
      AATIQELDM FADSDEANY GKFLDTLVR KRREIADSN 
      ANIPPPTQQ MKPSASSQQ VAPANGVQC DVKRSQSGP 
      IVIGAGKPI PYGNPSTKR VPQVSKSSS QSSGFISKI 
      GVDQVVEGG IPDLRRLEI SPITSVEEF CPDGGQIDR 
      SFLDDVQYN VQNSGKMEE NDSDSDTDT GNPLVSELQ 
      EDFDPDDLN SSSKKIGKS ELKAPAPLR IVRSDESSE 
      IEVDKTAPE DYDMKQTND EFDSTINSE ISELTSDAY 
      DGWMGSGED VSVVSQTLD YSNSTAYDD STSVTSSNV 
      HMELLSSKH SPVSANGSV NSDSEETHT SSSVKKEKK 
      KKEKSEKKH KAKKSKDKE KPKEKSERR HKRRSRDET 
      SSRRDELEE FLNGSASPP IDAAYEAI
    CDS sequence
    • >TC001511 RA
      atgttttcg gctataaaa cgttttgcg agtaaggga 
      gatggctcc cctagtaac tcgacggtc gtggggcgg 
      cccccgagt caccaggcg atgtccact tcgcttcag 
      agaaaattt gcgaggggt gtgcaatat aacatgaaa 
      attgtgatc aagggggac aggaatgtg ggcaagacg 
      tgtctgttc catcggctt caagggaaa aagttcata 
      gaggagtac atacccact gaggagatt caagtcact 
      tcgattcag tggaattac aaagcgact gatgatatt 
      gtcaaggtg gaagtgtgg gatgttgtg gaccgaggg 
      aagaagaag aagcggttt gaaggcttg aaactggag 
      aattcgcaa attgaaatg ccggaagag ccggccctg 
      gatgctgag tttttggac gtttacaaa gggactaac 
      ggggttatt atgatgctg gatttgact aaaacttgg 
      acgtttgac tacgtccag agggaacta ccgaaagtc 
      cctgcacac attccggtc attattttg gcaaatcat 
      tgtgatatg gggcaccac aggactgtc acttctgac 
      catgtcact ttttatatt gagtcaatt gcaagcacc 
      cgttcggcg caaattcgc tactcggag tcgtccatg 
      cgcaacggc ttcggcctg aagctcctc cacaaattc 
      ttcaacctc cccttcctc caactccag aaggagacc 
      ctcctccgc caactcgaa cgcaacgag atggagatc 
      gccgccacc atccaagaa ctcgacatg tttgcggat 
      tcggacgaa gcaaattac ggcaaattt ctcgatact 
      ttggtgcga aaacgtcgc gaaatcgcc gattccaac 
      gctaacatt ccgccaccg acgcaacaa atgaagccg 
      tcggcgtct agtcaacaa gtggccccc gcaaatggg 
      gttcaatgt gatgttaaa cggtcacag agcggcccc 
      attgtcatc ggggccgga aaaccgatc ccttatggg 
      aatcccagc acgaagaga gtgcctcaa gtatcgaaa 
      agttcaagt cagagttct gggtttatc agtaagatt 
      ggggtcgat caggtggtc gaaggggga atccctgat 
      ttgaggagg ttggagatt agtccgatt acgtcagtg 
      gaggagttt tgtccagat ggggggcag attgatagg 
      agttttttg gatgatgtg caatataat gtccagaat 
      agtggcaaa atggaggaa aatgactct gattcggac 
      acagacact ggcaatcct ctcgtctcc gaattacaa 
      gaagacttc gacccggac gatctcaat tcctcgtcg 
      aaaaaaatc ggaaaatcc gaattaaaa gcaccagct 
      ccgttacga atcgtccgt tcggacgaa agtagcgaa 
      attgaagtt gacaagact gcacctgaa gactatgat 
      atgaaacaa acaaacgac gaatttgat tcgactata 
      aattcggaa atttccgaa ctgacttcg gacgcttat 
      gacggctgg atgggcagt ggtgaagat gtgtcagtt 
      gtgagtcaa accctagat tacagtaat agtacggct 
      tatgatgat agtacgagt gtgacgtca tcgaatgta 
      catatggag ctgttgagt tcgaaacat agtccagtt 
      tcggcgaac ggaagtgtg aatagtgat agtgaagaa 
      actcacact tcgagttca gtcaagaaa gagaaaaag 
      aaaaaagag aagtcggaa aagaagcat aaggccaaa 
      aaatcaaaa gacaaggaa aaaccgaaa gaaaaaagc 
      gaacgtcgg cacaaacgt cggtcacgt gatgagact 
      tcatcccgt cgcgacgaa ttggaagag ttcttaaat 
      ggcagtgca agtccacca atcgatgca gcgtacgaa 
      gccatctga
    mRNA sequence
    • >TC001511 RA
      aataaatca aaataatta agtctgatt aataaacac 
      aatttatag tttttgtgg ctagttttc gtcaatata 
      gtaggtttt acttttttg gttgtcatc tgtcagttg 
      tgtcgaagg tcatgtaaa tagttttat cttaaattg 
      aatttggcg ataaataaa atgaaacaa tagttgttt 
      ttgtggccg tcgtgtgtg ccgcggtac ttgtgttgg 
      ttccatgca tgtgttcgt ccaataaaa tcgatctcc 
      attaaccat atggaaatc tggtcaagt gccaaacag 
      aaatgtttt cggctataa aacgttttg cgagtaagg 
      gagatggct cccctagta actcgacgg tcgtggggc 
      ggcccccga gtcaccagg cgatgtcca cttcgcttc 
      agagaaaat ttgcgaggg gtgtgcaat ataacatga 
      aaattgtga tcaaggggg acaggaatg tgggcaaga 
      cgtgtctgt tccatcggc ttcaaggga aaaagttca 
      tagaggagt acataccca ctgaggaga ttcaagtca 
      cttcgattc agtggaatt acaaagcga ctgatgata 
      ttgtcaagg tggaagtgt gggatgttg tggaccgag 
      ggaagaaga agaagcggt ttgaaggct tgaaactgg 
      agaattcgc aaattgaaa tgccggaag agccggccc 
      tggatgctg agtttttgg acgtttaca aagggacta 
      acggggtta ttatgatgc tggatttga ctaaaactt 
      ggacgtttg actacgtcc agagggaac taccgaaag 
      tccctgcac acattccgg tcattattt tggcaaatc 
      attgtgata tggggcacc acaggactg tcacttctg 
      accatgtca ctttttata ttgagtcaa ttgcaagca 
      cccgttcgg cgcaaattc gctactcgg agtcgtcca 
      tgcgcaacg gcttcggcc tgaagctcc tccacaaat 
      tcttcaacc tccccttcc tccaactcc agaaggaga 
      ccctcctcc gccaactcg aacgcaacg agatggaga 
      tcgccgcca ccatccaag aactcgaca tgtttgcgg 
      attcggacg aagcaaatt acggcaaat ttctcgata 
      ctttggtgc gaaaacgtc gcgaaatcg ccgattcca 
      acgctaaca ttccgccac cgacgcaac aaatgaagc 
      cgtcggcgt ctagtcaac aagtggccc ccgcaaatg 
      gggttcaat gtgatgtta aacggtcac agagcggcc 
      ccattgtca tcggggccg gaaaaccga tcccttatg 
      ggaatccca gcacgaaga gagtgcctc aagtatcga 
      aaagttcaa gtcagagtt ctgggttta tcagtaaga 
      ttggggtcg atcaggtgg tcgaagggg gaatccctg 
      atttgagga ggttggaga ttagtccga ttacgtcag 
      tggaggagt tttgtccag atggggggc agattgata 
      ggagttttt tggatgatg tgcaatata atgtccaga 
      atagtggca aaatggagg aaaatgact ctgattcgg 
      acacagaca ctggcaatc ctctcgtct ccgaattac 
      aagaagact tcgacccgg acgatctca attcctcgt 
      cgaaaaaaa tcggaaaat ccgaattaa aagcaccag 
      ctccgttac gaatcgtcc gttcggacg aaagtagcg 
      aaattgaag ttgacaaga ctgcacctg aagactatg 
      atatgaaac aaacaaacg acgaatttg attcgacta 
      taaattcgg aaatttccg aactgactt cggacgctt 
      atgacggct ggatgggca gtggtgaag atgtgtcag 
      ttgtgagtc aaaccctag attacagta atagtacgg 
      cttatgatg atagtacga gtgtgacgt catcgaatg 
      tacatatgg agctgttga gttcgaaac atagtccag 
      tttcggcga acggaagtg tgaatagtg atagtgaag 
      aaactcaca cttcgagtt cagtcaaga aagagaaaa 
      agaaaaaag agaagtcgg aaaagaagc ataaggcca 
      aaaaatcaa aagacaagg aaaaaccga aagaaaaaa 
      gcgaacgtc ggcacaaac gtcggtcac gtgatgaga 
      cttcatccc gtcgcgacg aattggaag agttcttaa 
      atggcagtg caagtccac caatcgatg cagcgtacg 
      aagccatct gagtacaat ttatcgcct aacgccttc 
      caattttat aatatgctt aaatatatt ttttaagtg 
      aatttattt aacgtgata tgtatgcta acatgcttt 
      gcataatta catcaattt aatcctgca tatgtttgg 
      gtgtgcaca cagtcaaat cttttccaa aaagtacaa 
      taatcgatt aggcgtaag tgcatgtgt gcctaaagt 
      gtttttacg aatcaatat ttttctaca ctccattcg 
      tagacccgt taacttatt gttacatgt tgtttttgt 
      tatgtgtac cacaatcat taatcgctt atttatttt 
      attattgtt tctctctaa cgattgttg atatttaag 
      acccgaacc tcaataaat aagtataac cgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY