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Gene information
General information
TC031732
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001493
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid999: 9106..13917
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031732
      iBeetle screen iB_03198
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      mesothoracic leg shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      mesothoracic leg potentially transformed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      Comment: Mesothoracic leg potentially transformed to a palp like strukture.
      abdominal segment 2 ectopically structure - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: Abdominal segment 2 shows an ectopically leg like structure.
      abdominal segment 1 ectopically structure - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      Comment: Abdominal segment 1 shows an ectopically leg like structure.
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031732 RA
        MSTRSSVNS PSRRFPPLP IRRPLLNRF 
        LNPVYTTRR FVPRANTAI ITNTPINTN 
        PLKHSGYSE EQARLQRLV DVLDEIPSD 
        PESIDEEED DVDEDIEGT YVIGTDNVL 
        QRVDNILQP EEHVPDING IEENDDNQS 
        QNDDHDVSD DDTPIAVRL YRKGITWSD 
        DYPNIARKN EFREHTGPN IEAEEPIDR 
        RTKPATLQV NFRQ
      CDS sequence
      • >TC031732 RA
        atgagtacc aggtcatcc gttaattct ccttcccgt 
        cgttttcct cctttgcca attcgtcgt ccgctattg 
        aaccggttt ttgaatcct gtttatact actcgtcgc 
        ttcgttccc agggctaat actgctatt ataactaac 
        actcctatt aatactaac cctttgaag cacagtggt 
        tactcagaa gagcaagct cgtcttcaa agacttgtt 
        gacgttcta gatgaaata ccatccgac ccagagtct 
        attgatgaa gaagaagat gacgtggat gaagatatt 
        gaaggcacg tatgtaatt ggtacagac aatgtatta 
        cagcgtgta gataacatt ttgcaacca gaagaacat 
        gttccagac attaatggc attgaagaa aatgatgac 
        aaccagtct cagaatgat gatcacgac gtttctgac 
        gacgatact cccattgct gttagactt tacaggaaa 
        ggaattact tggtctgat gattatcca aacatagca 
        agaaaaaat gagtttagg gaacataca ggtccaaac 
        atagaagca gaagaacct attgataga cgtacaaaa 
        cctgctacg ctccaagtg aatttcagg caatag
      mRNA sequence
      • >TC031732 RA
        atcaaacaa ataaattca cttacacaa gggaagata 
        actgctgga atcccatta tgttacatt ggaccacac 
        gattttcga aaaccttca cgtctgggg ttgaatatc 
        gccgtttgt cacctatgg tagcgacga tgcagcagt 
        attttctaa gaaaagggt tatgtgtga catttgaaa 
        gcaatttaa ggtgatatc agttctttt gggtaaaat 
        ataaatcca tgtattcgg tttatccaa tttatgcca 
        aatttctaa gcgagaggt tataattct tatgttgca 
        atttattgg gatattgag aggagattt taaaagttc 
        ggtttcaca tataggtct agaatttgt atagtagaa 
        aggttccga agttacaca aataaaagt tgattcatc 
        taaattttg acaattagt taactcatt taaacctaa 
        atacaaatt atggttttc ggagattgc tagataatt 
        gaatgacgg taaaataaa tttataata agaggttga 
        gttgtctaa aaccggtaa tggaattaa tttataaag 
        attaaattg atttcgatt tacaagagg tattgatag 
        aacatagcc aattctgtt gtcggtgaa gaaacattt 
        gattgtgag taattctaa atatttgtg tgcattttc 
        gcggctaac tggtaaagg atacgtaga tgaactagg 
        taaatgtgg aattgtttt actcaattc ttcgatcaa 
        ttgaaatgg tgttatgac ataagggtg taaaatgtt 
        tgatctaga aagtaaaat cgtgtccat aacggtcgt 
        tatttcgtt ttgtaatgt tgtaataac tagatttaa 
        taaagaaaa gtgttcgtc taaattttg ttttaattc 
        taatgcaag atacctatt gtttcgtct tttccttct 
        aaatttact cgttccgtt tttcaaagt tatttaaat 
        tatactcgc gatttttct agttcaaaa atttatttc 
        ttttcaaaa aataattcc tgttttaat tttgttaat 
        actactata tttacaaat attataact gataatttt 
        gaatttttc ttttctaat agtgttcgt ttggtatca 
        tctgttaat caattaaac caaatttca ccgatactt 
        ttctcagaa gacgattgc gggtactat tttgaacca 
        gccgctaca tatccaaca tcacccaat cttatcctt 
        tatttagct attatctcc ttaattttt gcttccttt 
        tgtatatat cacattttc aaaattata acttcgcat 
        aatgagtac caggtcatc cgttaattc tccttcccg 
        tcgttttcc tcctttgcc aattcgtcg tccgctatt 
        gaaccggtt tttgaatcc tgtttatac tactcgtcg 
        cttcgttcc cagggctaa tactgctat tataactaa 
        cactcctat taatactaa ccctttgaa gcacagtgg 
        ttactcaga agagcaagc tcgtcttca aagacttgt 
        tgacgttct agatgaaat accatccga cccagagtc 
        tattgatga agaagaaga tgacgtgga tgaagatat 
        tgaaggcac gtatgtaat tggtacaga caatgtatt 
        acagcgtgt agataacat tttgcaacc agaagaaca 
        tgttccaga cattaatgg cattgaaga aaatgatga 
        caaccagtc tcagaatga tgatcacga cgtttctga 
        cgacgatac tcccattgc tgttagact ttacaggaa 
        aggaattac ttggtctga tgattatcc aaacatagc 
        aagaaaaaa tgagtttag ggaacatac aggtccaaa 
        catagaagc agaagaacc tattgatag acgtacaaa 
        acctgctac gctccaagt gaatttcag gcaatagat 
        gaatcgatg attccgttc aagggcaga agctgtctc 
        aaacaatat atgcccgat aaacccact aagcgaggt 
        tataaggtg tgggtacgt gcagatagt tatggttac 
        gttgtacaa tttcaaatt tacgaagga aaaagaaaa 
        gatatcgta gaaaaaaat cttggactt cgagtagtc 
        aaagaattg acaatagac ttagttggc aaaaattac 
        tcagtatat ttcgataat tttttcagt tcagtagaa 
        ctacttatt tatctccaa caaaataaa atcaatgca 
        gctgctact accagacca aatagaaag aattttcca 
        aaagatttt ccaactgac aaagaaatg attcgtggc 
        aattttgct tggagatca acacgtact ggtataacg 
        gctacaaaa tggaaagac agaaaagga atacatttt 
        ctttcaaac atgcacagt ccattgaca gaaacttca 
        gtgaggaga aaagaacac gacggttca acgaaacaa 
        atcgcttgt ccagcagtt gttcaggac tacaacaaa 
        cacatgggt tgtgtggac aaagcagac atgttaaaa 
        acaatatat tgtattgat agaaagtcg cgcaaatgg 
        tggcaccgt atatttttc cacatgttg gacacgacg 
        gtggtaaat acatttatc atctactca caacttacg 
        gaaggaaaa aagcttaca ctcaagcaa ttgcgcctt 
        gctattgca atatctctt atcggcatt ccacagaga 
        gaaaaagtt ggacgtaaa tcatcaacc cctgttcac 
        cattttaaa acttacgtc tcacaagaa gtaagatac 
        aacaagtcg gaacatttg cctgtcaga acaacatct 
        cgaagatgt ggacattgt tccacagta gcagaagtt 
        cataggaca aaatgggct tgctccgta tgcaaagtt 
        gccctatgc ctctctgat aacaggaac tgttttctt 
        ggatttcat acaaaataa ataatattt agattt