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Gene information
General information
TC001452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 3512555..3514899
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001452
    iBeetle screen iB_03191
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001452 RA
      MPTYCETPP EHLQSKTII VIHPGSLNL RIGRASDLN 
      PLTILHAIA RRCLNKKYQ YRDSFLPPR IDLSQPQEF 
      EDARLSVSH TLQSCLQSD GRRRYATPP QQIAAFNRR 
      AKPEILGPS GGEWLKVEG DTVIGNDIL KLDPKETFN 
      IHFPFKRGD FNIHPGPGG SMTSVLVDL ETIWTHVLE 
      THFQIEKKD FKHHKAVLI IPDVYNRTF LRELMYLLL 
      QKMGFGSCF LVQDHVAAT FGSGLSYAC VVDVGDQKT 
      SVSCVEDGI CHPNTRVRL EYGGGDITQ IFYWLLQKC 
      AFPYKECND QNKLDSMLL RKLKEDFCH VNLDICGSQ 
      EKTLILKQP GKPILKYTI QVGDECIVA PLSMFSPEL 
      FGITGVKTI RTQKASSGD PEDPHDENY LRDIRRKGM 
      KDNMEPSTS DLLSEPYLE QSQNPTNED DVVVDAIDT 
      VAPSYDKEF VIAPGQILG LDQAVLQSI DRCPNEDLK 
      RKMYGCILI VGGGMKFSG IGTWLQNRI SLQIPYLYR 
      AEQLDIITN PRDMDPAMV AWKGACIMS CLETAHELW 
      ILASEWEKY GVRVLRERA PFTW
    CDS sequence
    • >TC001452 RA
      atgccaacg tattgcgaa acgcctcca gagcacctc 
      cagtctaag acaattata gtgatacat cctggctct 
      ttaaacctc cgaataggg cgtgcgtcc gacctgaac 
      cccttgacg attttacac gccatagct cgccgttgt 
      ctgaataaa aaataccag tatagggac tcattcctc 
      cccccgcga atagatttg agccagcct caagagttc 
      gaagacgcc cgtctttcc gtctcccac actttgcag 
      tcgtgtctg caatcggac ggccgccgg cgctatgcg 
      acgccccct cagcaaatc gccgccttc aaccgccgg 
      gccaaaccc gagatcttg gggcccagt ggcggcgaa 
      tggctcaaa gtcgaaggg gacacagtg ataggaaac 
      gacattttg aaactcgac cctaaagaa accttcaac 
      attcatttc ccgttcaaa aggggcgac tttaacata 
      catccaggg ccggggggc tccatgact tcagtatta 
      gtagactta gaaaccatc tggacgcac gtcctcgag 
      acgcacttc cagatcgag aaaaaagat ttcaaacac 
      cacaaagcc gtgcttatt ataccggac gtctataat 
      aggacgttc ctgagggaa ctaatgtac ttattattg 
      cagaaaatg gggttcggg agctgtttt ctagttcaa 
      gatcatgtt gctgccact tttggctca ggtttaagt 
      tacgcgtgc gtggtggat gtgggggac cagaaaact 
      tcggtttcg tgcgttgag gacggtatt tgtcacccc 
      aataccagg gttcggcta gagtatgga ggtggcgac 
      atcacacag attttctac tggttgtta caaaagtgt 
      gcttttcct tacaaagaa tgtaacgac cagaataag 
      ctagattcg atgttactg cgcaaattg aaggaagat 
      ttctgccat gttaacttg gatatttgt ggctcgcaa 
      gaaaagact ttgattttg aaacagccg ggaaagcca 
      attttgaaa tacaccatc caagtgggc gatgagtgt 
      atagtggcg ccgcttagc atgtttagc ccggaacta 
      ttcggaata acgggcgtt aagactata aggactcag 
      aaggcgtca agtggggat ccggaggat ccccacgat 
      gaaaactac ttgcgtgat ataagacgg aaaggaatg 
      aaagataac atggaaccg agcacttcc gatctgctc 
      agtgaacca tacttagag caatcacaa aatcccaca 
      aacgaggat gatgttgtt gtggatgcg atcgatacg 
      gtggcaccc agctatgac aaagagttt gtgatcgcg 
      ccaggtcag atcttgggt cttgaccag gcggttctg 
      caaagtatc gacagatgt cccaatgag gacctcaag 
      cgcaaaatg tacggttgt attttaata gttggcggc 
      gggatgaaa ttttcagga atcgggacg tggcttcag 
      aaccgcatt tcacttcaa attccatac ttataccgc 
      gcggaacag cttgatatt ataacaaat cccagagat 
      atggacccg gccatggta gcatggaaa ggcgcttgc 
      ataatgtcc tgtctggag acggctcat gaattgtgg 
      attttggcc tctgagtgg gaaaaatac ggggtgcga 
      gtgttgcgg gaaagggcg cctttcaca tggtga
    mRNA sequence
    • >TC001452 RA
      gcactaaga tatctaaaa cgtgttcgc ccccttaaa 
      aattaactg ctttgtctc gtagttaaa tatttgaat 
      cgtatctcc tgaactcat gtagcttat aaaattaat 
      ccggtaccg gtattaaaa agtgtggtt atgtaacta 
      ttccagcta aaaagtgtt cgttgtttt ttcttttta 
      atgatgcca acgtattgc gaaacgcct ccagagcac 
      ctccagtct aagacaatt atagtgata catcctggc 
      tctttaaac ctccgaata gggcgtgcg tccgacctg 
      aaccccttg acgatttta cacgccata gctcgccgt 
      tgtctgaat aaaaaatac cagtatagg gactcattc 
      ctccccccg cgaatagat ttgagccag cctcaagag 
      ttcgaagac gcccgtctt tccgtctcc cacactttg 
      cagtcgtgt ctgcaatcg gacggccgc cggcgctat 
      gcgacgccc cctcagcaa atcgccgcc ttcaaccgc 
      cgggccaaa cccgagatc ttggggccc agtggcggc 
      gaatggctc aaagtcgaa ggggacaca gtgatagga 
      aacgacatt ttgaaactc gaccctaaa gaaaccttc 
      aacattcat ttcccgttc aaaaggggc gactttaac 
      atacatcca gggccgggg ggctccatg acttcagta 
      ttagtagac ttagaaacc atctggacg cacgtcctc 
      gagacgcac ttccagatc gagaaaaaa gatttcaaa 
      caccacaaa gccgtgctt attataccg gacgtctat 
      aataggacg ttcctgagg gaactaatg tacttatta 
      ttgcagaaa atggggttc gggagctgt tttctagtt 
      caagatcat gttgctgcc acttttggc tcaggttta 
      agttacgcg tgcgtggtg gatgtgggg gaccagaaa 
      acttcggtt tcgtgcgtt gaggacggt atttgtcac 
      cccaatacc agggttcgg ctagagtat ggaggtggc 
      gacatcaca cagattttc tactggttg ttacaaaag 
      tgtgctttt ccttacaaa gaatgtaac gaccagaat 
      aagctagat tcgatgtta ctgcgcaaa ttgaaggaa 
      gatttctgc catgttaac ttggatatt tgtggctcg 
      caagaaaag actttgatt ttgaaacag ccgggaaag 
      ccaattttg aaatacacc atccaagtg ggcgatgag 
      tgtatagtg gcgccgctt agcatgttt agcccggaa 
      ctattcgga ataacgggc gttaagact ataaggact 
      cagaaggcg tcaagtggg gatccggag gatccccac 
      gatgaaaac tacttgcgt gatataaga cggaaagga 
      atgaaagat aacatggaa ccgagcact tccgatctg 
      ctcagtgaa ccatactta gagcaatca caaaatccc 
      acaaacgag gatgatgtt gttgtggat gcgatcgat 
      acggtggca cccagctat gacaaagag tttgtgatc 
      gcgccaggt cagatcttg ggtcttgac caggcggtt 
      ctgcaaagt atcgacaga tgtcccaat gaggacctc 
      aagcgcaaa atgtacggt tgtatttta atagttggc 
      ggcgggatg aaattttca ggaatcggg acgtggctt 
      cagaaccgc atttcactt caaattcca tacttatac 
      cgcgcggaa cagcttgat attataaca aatcccaga 
      gatatggac ccggccatg gtagcatgg aaaggcgct 
      tgcataatg tcctgtctg gagacggct catgaattg 
      tggattttg gcctctgag tgggaaaaa tacggggtg 
      cgagtgttg cgggaaagg gcgcctttc acatggtga 
      tttgtttcg aaataaagt tttgcacga gta