Status
Please wait ...
Gene information
General information
TC001442 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3885135..3890886
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001442
    iBeetle screen iB_03189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001442 RA
      MYSEEQDSH GLLTLHYGK HHATIVDEI KTCFASENF 
      ADMTFVCDD KTTLSAHKL IMAAASPLV RRILGESAH 
      AHGPSVVLI PGIKSCHLR HLLDFLYNG QACVKSSEL 
      DSIQELFEL LQIKSDMWQ SSSTEEAKC QSEHGNSYD 
      NSDLMVVKR ETDDDEKEE GEIKDDDDD TLDENNISE 
      EGGSSPNPV NLSLNTKNT DEEKDIDDD NSSEQLEKK 
      NSDVLKIGQ TTVKIAPKV HELSQFHHH RMKRKSMYI 
      EPIDLKNQE ELKPPIGDQ KLISLVQSP DNYVVTPHR 
      KRRPGFHNS PAQNPPFVP FSPSYIDDA VHHQHHRYK 
      NLPPLIAAH HPLSLSAPP FLVDRIHTP TPGQHSDTV 
      GPPDNVVVK YRPPSADQG VTEAYQNFE HTWGAWSLC 
      QTQVNPSSS NEDSRDPPA SFSVSGGGG GGGSGVSSA 
      GGGDSAESS SHASITTKQ VLPTVQVRE YRCEYCGKQ 
      FGMSWNLKT HLRVHTGEK PFACRLCVA MFKQKAHLL 
      KHLCSVHRN VISSNDGNS GRFNCCFCP LSFESLPEL 
      IRHLSGPHN NLLLSKNMH ELK
    CDS sequence
    • >TC001442 RA
      atgtactct gaggaacag gattcccac ggcttatta 
      accttgcat tatggcaaa catcatgca actatagtc 
      gacgaaatt aaaacttgt tttgcctct gagaacttt 
      gccgatatg acattcgta tgtgacgat aaaacaact 
      ttaagcgct cataaatta ataatggcg gcagcgagt 
      cctttagtg cgacgcatt cttggagag agcgcccat 
      gctcacggg cccagcgtt gtgctcatc ccaggaata 
      aaaagctgc cacctccgg cacttgctc gactttttg 
      tacaacggg caagcttgc gttaagtct agcgaattg 
      gacagcatt caggagttg ttcgaactg ttgcaaatt 
      aaatcggac atgtggcag tcttcgtcg accgaagag 
      gccaagtgt cagtccgaa cacggcaac agttacgac 
      aattccgac ttgatggtt gtaaaaaga gaaacggac 
      gacgacgaa aaggaagaa ggcgaaatt aaagacgac 
      gacgatgac actttggac gaaaacaat atttcagaa 
      gaaggcgga tcctcgcca aacccagtg aatctatct 
      ctcaacacg aaaaacacc gacgaggaa aaggacatc 
      gacgatgac aacagctcg gagcagttg gaaaagaaa 
      aattccgac gtgttgaaa atcggccag acgactgtg 
      aaaatcgcc ccgaaagtg cacgaattg tcgcagttc 
      caccaccac cgaatgaaa cgcaaaagt atgtacatt 
      gagccgatc gatttgaag aaccaggaa gaattgaag 
      ccgccgatc ggcgaccag aagctgatc tcgttggtg 
      caaagtccc gacaattac gttgtgact ccgcatcgc 
      aaacggcgt cctggtttc cacaactcc ccggctcaa 
      aacccccca tttgtgccg ttttcgccg tcttacatc 
      gacgacgcc gtgcaccac cagcaccac agatacaag 
      aatttgccg cctttgatc gcagcccac cacccgtta 
      tcgctgtcg gcgccgccg tttctggtg gataggatc 
      cacactccg acgccgggg caacactcc gacaccgtg 
      ggccccccg gacaacgtc gtggtcaag tacaggccg 
      cccagcgcg gaccagggg gtgacggag gcgtaccag 
      aattttgag cacacttgg ggcgcctgg tcgctgtgc 
      cagacgcaa gtgaatccg tcttcgagc aatgaggat 
      tcgagggat ccgccggcg tctttctca gttagtggt 
      ggtggcggt ggtgggggc agtggggtt tcgagtgcc 
      gggggcggt gattcggcg gaaagttcg tcgcatgcg 
      tctatcacc acgaaacaa gtgctgccc acggtgcaa 
      gttcgggag taccggtgt gagtactgt ggcaaacag 
      tttgggatg tcctggaat ttgaagacc cacttgcgg 
      gtgcacacc ggcgaaaaa ccgttcgct tgtcggttg 
      tgtgtcgcg atgttcaag cagaaagca catttattg 
      aaacatctg tgttcagtg catcggaac gttatatcg 
      tcgaacgat ggcaattcc ggacgtttt aactgttgt 
      ttctgcccc ttgtcgttt gagtcccta cccgaattg 
      atcaggcat ttgtcagga ccgcacaat aatttactt 
      ctaagtaaa aacatgcac gagcttaaa taa
    mRNA sequence
    • >TC001442 RA
      acaaattgt tgttcggac attattata atgttgctt 
      ggtgattat aattatgat ttttacctg agaggatca 
      agtaagtac aaagtgatt tgaggcgag gagagcgga 
      tgttcaaag tttcaagga tgctttgat cctgcagtc 
      tcggctcga cttagcgcg gctctaaca attaccaac 
      catgtgact atcgaccca tatccaatt atgtaaaac 
      ctccaagtt tacgttaat ggtccttaa attttttaa 
      tagggacat tggtgtctg gccgactaa ttgtcccta 
      aagatgttg cgttgcgct gtttttgat gttgtttgt 
      agccgtgtc agttcaacc aagaccact ttcacttcc 
      ctacaaagc ggagacctt gaaggttta aacaggtaa 
      aatcagtct cgaggacag ccgaccaac tacacaaaa 
      agattcatt acttaataa cgccaaact cacatgaac 
      gcgatttgg ggttgtacc ggtggctat tacctatta 
      ttctaagtg ggaatgcga atgaatata ttaggccag 
      agtggttgg caattagct gtgtggatg agactggtt 
      tacggcttg tggccatta ttcattgcg atgatgcga 
      tgtgtcata aagtctttg gaggtcgaa ttctacatt 
      taaggtcca gtttcagac agacattac gcgatagtg 
      aaagtgcta cgctatgcc tactttccc tcgctcaca 
      taacgtaat actaacata acaatagat gcctattac 
      aggaatgcc tggcgcttg aattcggtg gcattttcg 
      ggcagagac tggcacaag gggtggagt gagtgggac 
      aaagcaggc tggtttggc cgggtccgg gagcgcctc 
      cagtggctg aaaattggg caaaagagg aaaaaaagc 
      ataggtctt tctatttct agcggctgc tatttcatc 
      gatattata caaccgttc cccccctcg gctctcggc 
      cgtcggtat tgtgaaagt ccggttgtg gcccctgtt 
      tgggccccg ctcgcgacg agtctcctt gggaagccg 
      gtgaaagca attgaaacg gtctctgca acagtcgaa 
      gtgacgaat gcagaatta caccatccg ctcgagcgg 
      cgtactatc cgtccctaa acgaaaata gcctctatg 
      gcgaaactt cgtgtcact ttgccaaag attcgaaat 
      agtccggga gagcaaaaa tgcgctaaa ttgcgcgca 
      gccgcaagt ggcgtctcc agctttcga ccttgaaaa 
      tgagaaaga gaagttcca agtgagcta gcaatagga 
      aaaaactcg cgatgtagt ggggaggca agagaaaag 
      aggggaaag taaagggag aaattaaga gttagtagt 
      attacgttc gaacccaac ccgtgagca aagccgatg 
      gttctgaat atttcgcag caaagcgca acttttgag 
      ggtggcagt ggtcgcaag cgtcgcgcc gacaccgcc 
      gaaaccgtt ttagggctt gaggaaact tgcttcaat 
      aagcaccgt tgtgttgtg gtgttgtga aggctcgtg 
      ttcgttcac ttttacttt catttgtgg cgcaacaca 
      gtgtttgag agtcgtgtc ccaaaaata taattcact 
      caacgcgaa ggctggcga ccgatcaaa cacggtttt 
      atgtgcttg aacgttcga attatggtg cggtgtgat 
      tattgcaca tcttctggt gctcgcgac tgccctacg 
      gcgaccaca gtcgcgagt gccctttag gactattta 
      ttctctggg ttgtccttg acaaaatta ctaaattcg 
      ccatgtact ctgaggaac aggattccc acggcttat 
      taaccttgc attatggca aacatcatg caactatag 
      tcgacgaaa ttaaaactt gttttgcct ctgagaact 
      ttgccgata tgacattcg tatgtgacg ataaaacaa 
      ctttaagcg ctcataaat taataatgg cggcagcga 
      gtcctttag tgcgacgca ttcttggag agagcgccc 
      atgctcacg ggcccagcg ttgtgctca tcccaggaa 
      taaaaagct gccacctcc ggcacttgc tcgactttt 
      tgtacaacg ggcaagctt gcgttaagt ctagcgaat 
      tggacagca ttcaggagt tgttcgaac tgttgcaaa 
      ttaaatcgg acatgtggc agtcttcgt cgaccgaag 
      aggccaagt gtcagtccg aacacggca acagttacg 
      acaattccg acttgatgg ttgtaaaaa gagaaacgg 
      acgacgacg aaaaggaag aaggcgaaa ttaaagacg 
      acgacgatg acactttgg acgaaaaca atatttcag 
      aagaaggcg gatcctcgc caaacccag tgaatctat 
      ctctcaaca cgaaaaaca ccgacgagg aaaaggaca 
      tcgacgatg acaacagct cggagcagt tggaaaaga 
      aaaattccg acgtgttga aaatcggcc agacgactg 
      tgaaaatcg ccccgaaag tgcacgaat tgtcgcagt 
      tccaccacc accgaatga aacgcaaaa gtatgtaca 
      ttgagccga tcgatttga agaaccagg aagaattga 
      agccgccga tcggcgacc agaagctga tctcgttgg 
      tgcaaagtc ccgacaatt acgttgtga ctccgcatc 
      gcaaacggc gtcctggtt tccacaact ccccggctc 
      aaaaccccc catttgtgc cgttttcgc cgtcttaca 
      tcgacgacg ccgtgcacc accagcacc acagataca 
      agaatttgc cgcctttga tcgcagccc accacccgt 
      tatcgctgt cggcgccgc cgtttctgg tggatagga 
      tccacactc cgacgccgg ggcaacact ccgacaccg 
      tgggccccc cggacaacg tcgtggtca agtacaggc 
      cgcccagcg cggaccagg gggtgacgg aggcgtacc 
      agaattttg agcacactt ggggcgcct ggtcgctgt 
      gccagacgc aagtgaatc cgtcttcga gcaatgagg 
      attcgaggg atccgccgg cgtctttct cagttagtg 
      gtggtggcg gtggtgggg gcagtgggg tttcgagtg 
      ccgggggcg gtgattcgg cggaaagtt cgtcgcatg 
      cgtctatca ccacgaaac aagtgctgc ccacggtgc 
      aagttcggg agtaccggt gtgagtact gtggcaaac 
      agtttggga tgtcctgga atttgaaga cccacttgc 
      gggtgcaca ccggcgaaa aaccgttcg cttgtcggt 
      tgtgtgtcg cgatgttca agcagaaag cacatttat 
      tgaaacatc tgtgttcag tgcatcgga acgttatat 
      cgtcgaacg atggcaatt ccggacgtt ttaactgtt 
      gtttctgcc ccttgtcgt ttgagtccc tacccgaat 
      tgatcaggc atttgtcag gaccgcaca ataatttac 
      ttctaagta aaaacatgc acgagctta aataacatt 
      ccatgattg catgaggtc gtatattgt ggttgtgga 
      acgaccgca tcaatttcg attcgttga attgtgata 
      taattaaat gagataatc ccaatagtg cctttccta 
      tgaacataa tgcgtaact gttagctcg gcaaccctc 
      agggatgta gtctttagt tttagtcct gggaacggg 
      cactaaagc aaggggacc tgtcgtgcc tggacttga 
      ccgacgcct tccttcatt attgttata gtttattaa 
      ttagtagaa acaatctca accgttctt gtgttcaat 
      gattatcgt acttagtgc tatgcgtgg tgtgtatga 
      caaataatg attgtgtac gtacttttt tcacagaaa 
      gctaaactc tattgttat atacatcac aagtggatg 
      catattata cagtaggta catttacta aaaatgctg 
      ttctctcca agcattcgt ccaaagcat ttatagttt 
      atgtataag aaattgtat aatttttcc cgatttata 
      gtttttact acaaaactg aaatagata aacgtatat 
      tttttatat acacaccaa agaattaaa aattcactt 
      taagcgaat aaaactata tatttaaac tatcacctc 
      tttaaaaaa gtgctctcg ttttatatt ccgtgaatg 
      gaacaaagg ccattaaag tagtttttg tagaaaaga 
      tcatccgca aaaatactc aactcgccc gaaatttac 
      caaattcaa acgtgttgg cttacgact tcatacaaa 
      atgtggtac aaacatggt aaagtaata gttgtgtta 
      attttgtac atagtagtt tgtaaataa tgtatttac 
      aaaataaaa tagtctttt tcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY