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Gene information
General information
TC001442 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3885135..3890886
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001442
    iBeetle screen iB_03189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001442 RA
      MYSEEQDSH GLLTLHYGK HHATIVDEI KTCFASENF 
ADMTFVCDD KTTLSAHKL IMAAASPLV RRILGESAH 
AHGPSVVLI PGIKSCHLR HLLDFLYNG QACVKSSEL 
DSIQELFEL LQIKSDMWQ SSSTEEAKC QSEHGNSYD 
NSDLMVVKR ETDDDEKEE GEIKDDDDD TLDENNISE 
EGGSSPNPV NLSLNTKNT DEEKDIDDD NSSEQLEKK 
NSDVLKIGQ TTVKIAPKV HELSQFHHH RMKRKSMYI 
EPIDLKNQE ELKPPIGDQ KLISLVQSP DNYVVTPHR 
KRRPGFHNS PAQNPPFVP FSPSYIDDA VHHQHHRYK 
NLPPLIAAH HPLSLSAPP FLVDRIHTP TPGQHSDTV 
GPPDNVVVK YRPPSADQG VTEAYQNFE HTWGAWSLC 
QTQVNPSSS NEDSRDPPA SFSVSGGGG GGGSGVSSA 
GGGDSAESS SHASITTKQ VLPTVQVRE YRCEYCGKQ 
FGMSWNLKT HLRVHTGEK PFACRLCVA MFKQKAHLL 
KHLCSVHRN VISSNDGNS GRFNCCFCP LSFESLPEL 
IRHLSGPHN NLLLSKNMH ELK
    CDS sequence
    • >TC001442 RA
      atgtactct gaggaacag gattcccac ggcttatta 
accttgcat tatggcaaa catcatgca actatagtc 
gacgaaatt aaaacttgt tttgcctct gagaacttt 
gccgatatg acattcgta tgtgacgat aaaacaact 
ttaagcgct cataaatta ataatggcg gcagcgagt 
cctttagtg cgacgcatt cttggagag agcgcccat 
gctcacggg cccagcgtt gtgctcatc ccaggaata 
aaaagctgc cacctccgg cacttgctc gactttttg 
tacaacggg caagcttgc gttaagtct agcgaattg 
gacagcatt caggagttg ttcgaactg ttgcaaatt 
aaatcggac atgtggcag tcttcgtcg accgaagag 
gccaagtgt cagtccgaa cacggcaac agttacgac 
aattccgac ttgatggtt gtaaaaaga gaaacggac 
gacgacgaa aaggaagaa ggcgaaatt aaagacgac 
gacgatgac actttggac gaaaacaat atttcagaa 
gaaggcgga tcctcgcca aacccagtg aatctatct 
ctcaacacg aaaaacacc gacgaggaa aaggacatc 
gacgatgac aacagctcg gagcagttg gaaaagaaa 
aattccgac gtgttgaaa atcggccag acgactgtg 
aaaatcgcc ccgaaagtg cacgaattg tcgcagttc 
caccaccac cgaatgaaa cgcaaaagt atgtacatt 
gagccgatc gatttgaag aaccaggaa gaattgaag 
ccgccgatc ggcgaccag aagctgatc tcgttggtg 
caaagtccc gacaattac gttgtgact ccgcatcgc 
aaacggcgt cctggtttc cacaactcc ccggctcaa 
aacccccca tttgtgccg ttttcgccg tcttacatc 
gacgacgcc gtgcaccac cagcaccac agatacaag 
aatttgccg cctttgatc gcagcccac cacccgtta 
tcgctgtcg gcgccgccg tttctggtg gataggatc 
cacactccg acgccgggg caacactcc gacaccgtg 
ggccccccg gacaacgtc gtggtcaag tacaggccg 
cccagcgcg gaccagggg gtgacggag gcgtaccag 
aattttgag cacacttgg ggcgcctgg tcgctgtgc 
cagacgcaa gtgaatccg tcttcgagc aatgaggat 
tcgagggat ccgccggcg tctttctca gttagtggt 
ggtggcggt ggtgggggc agtggggtt tcgagtgcc 
gggggcggt gattcggcg gaaagttcg tcgcatgcg 
tctatcacc acgaaacaa gtgctgccc acggtgcaa 
gttcgggag taccggtgt gagtactgt ggcaaacag 
tttgggatg tcctggaat ttgaagacc cacttgcgg 
gtgcacacc ggcgaaaaa ccgttcgct tgtcggttg 
tgtgtcgcg atgttcaag cagaaagca catttattg 
aaacatctg tgttcagtg catcggaac gttatatcg 
tcgaacgat ggcaattcc ggacgtttt aactgttgt 
ttctgcccc ttgtcgttt gagtcccta cccgaattg 
atcaggcat ttgtcagga ccgcacaat aatttactt 
ctaagtaaa aacatgcac gagcttaaa taa
    mRNA sequence
    • >TC001442 RA
      acaaattgt tgttcggac attattata atgttgctt 
ggtgattat aattatgat ttttacctg agaggatca 
agtaagtac aaagtgatt tgaggcgag gagagcgga 
tgttcaaag tttcaagga tgctttgat cctgcagtc 
tcggctcga cttagcgcg gctctaaca attaccaac 
catgtgact atcgaccca tatccaatt atgtaaaac 
ctccaagtt tacgttaat ggtccttaa attttttaa 
tagggacat tggtgtctg gccgactaa ttgtcccta 
aagatgttg cgttgcgct gtttttgat gttgtttgt 
agccgtgtc agttcaacc aagaccact ttcacttcc 
ctacaaagc ggagacctt gaaggttta aacaggtaa 
aatcagtct cgaggacag ccgaccaac tacacaaaa 
agattcatt acttaataa cgccaaact cacatgaac 
gcgatttgg ggttgtacc ggtggctat tacctatta 
ttctaagtg ggaatgcga atgaatata ttaggccag 
agtggttgg caattagct gtgtggatg agactggtt 
tacggcttg tggccatta ttcattgcg atgatgcga 
tgtgtcata aagtctttg gaggtcgaa ttctacatt 
taaggtcca gtttcagac agacattac gcgatagtg 
aaagtgcta cgctatgcc tactttccc tcgctcaca 
taacgtaat actaacata acaatagat gcctattac 
aggaatgcc tggcgcttg aattcggtg gcattttcg 
ggcagagac tggcacaag gggtggagt gagtgggac 
aaagcaggc tggtttggc cgggtccgg gagcgcctc 
cagtggctg aaaattggg caaaagagg aaaaaaagc 
ataggtctt tctatttct agcggctgc tatttcatc 
gatattata caaccgttc cccccctcg gctctcggc 
cgtcggtat tgtgaaagt ccggttgtg gcccctgtt 
tgggccccg ctcgcgacg agtctcctt gggaagccg 
gtgaaagca attgaaacg gtctctgca acagtcgaa 
gtgacgaat gcagaatta caccatccg ctcgagcgg 
cgtactatc cgtccctaa acgaaaata gcctctatg 
gcgaaactt cgtgtcact ttgccaaag attcgaaat 
agtccggga gagcaaaaa tgcgctaaa ttgcgcgca 
gccgcaagt ggcgtctcc agctttcga ccttgaaaa 
tgagaaaga gaagttcca agtgagcta gcaatagga 
aaaaactcg cgatgtagt ggggaggca agagaaaag 
aggggaaag taaagggag aaattaaga gttagtagt 
attacgttc gaacccaac ccgtgagca aagccgatg 
gttctgaat atttcgcag caaagcgca acttttgag 
ggtggcagt ggtcgcaag cgtcgcgcc gacaccgcc 
gaaaccgtt ttagggctt gaggaaact tgcttcaat 
aagcaccgt tgtgttgtg gtgttgtga aggctcgtg 
ttcgttcac ttttacttt catttgtgg cgcaacaca 
gtgtttgag agtcgtgtc ccaaaaata taattcact 
caacgcgaa ggctggcga ccgatcaaa cacggtttt 
atgtgcttg aacgttcga attatggtg cggtgtgat 
tattgcaca tcttctggt gctcgcgac tgccctacg 
gcgaccaca gtcgcgagt gccctttag gactattta 
ttctctggg ttgtccttg acaaaatta ctaaattcg 
ccatgtact ctgaggaac aggattccc acggcttat 
taaccttgc attatggca aacatcatg caactatag 
tcgacgaaa ttaaaactt gttttgcct ctgagaact 
ttgccgata tgacattcg tatgtgacg ataaaacaa 
ctttaagcg ctcataaat taataatgg cggcagcga 
gtcctttag tgcgacgca ttcttggag agagcgccc 
atgctcacg ggcccagcg ttgtgctca tcccaggaa 
taaaaagct gccacctcc ggcacttgc tcgactttt 
tgtacaacg ggcaagctt gcgttaagt ctagcgaat 
tggacagca ttcaggagt tgttcgaac tgttgcaaa 
ttaaatcgg acatgtggc agtcttcgt cgaccgaag 
aggccaagt gtcagtccg aacacggca acagttacg 
acaattccg acttgatgg ttgtaaaaa gagaaacgg 
acgacgacg aaaaggaag aaggcgaaa ttaaagacg 
acgacgatg acactttgg acgaaaaca atatttcag 
aagaaggcg gatcctcgc caaacccag tgaatctat 
ctctcaaca cgaaaaaca ccgacgagg aaaaggaca 
tcgacgatg acaacagct cggagcagt tggaaaaga 
aaaattccg acgtgttga aaatcggcc agacgactg 
tgaaaatcg ccccgaaag tgcacgaat tgtcgcagt 
tccaccacc accgaatga aacgcaaaa gtatgtaca 
ttgagccga tcgatttga agaaccagg aagaattga 
agccgccga tcggcgacc agaagctga tctcgttgg 
tgcaaagtc ccgacaatt acgttgtga ctccgcatc 
gcaaacggc gtcctggtt tccacaact ccccggctc 
aaaaccccc catttgtgc cgttttcgc cgtcttaca 
tcgacgacg ccgtgcacc accagcacc acagataca 
agaatttgc cgcctttga tcgcagccc accacccgt 
tatcgctgt cggcgccgc cgtttctgg tggatagga 
tccacactc cgacgccgg ggcaacact ccgacaccg 
tgggccccc cggacaacg tcgtggtca agtacaggc 
cgcccagcg cggaccagg gggtgacgg aggcgtacc 
agaattttg agcacactt ggggcgcct ggtcgctgt 
gccagacgc aagtgaatc cgtcttcga gcaatgagg 
attcgaggg atccgccgg cgtctttct cagttagtg 
gtggtggcg gtggtgggg gcagtgggg tttcgagtg 
ccgggggcg gtgattcgg cggaaagtt cgtcgcatg 
cgtctatca ccacgaaac aagtgctgc ccacggtgc 
aagttcggg agtaccggt gtgagtact gtggcaaac 
agtttggga tgtcctgga atttgaaga cccacttgc 
gggtgcaca ccggcgaaa aaccgttcg cttgtcggt 
tgtgtgtcg cgatgttca agcagaaag cacatttat 
tgaaacatc tgtgttcag tgcatcgga acgttatat 
cgtcgaacg atggcaatt ccggacgtt ttaactgtt 
gtttctgcc ccttgtcgt ttgagtccc tacccgaat 
tgatcaggc atttgtcag gaccgcaca ataatttac 
ttctaagta aaaacatgc acgagctta aataacatt 
ccatgattg catgaggtc gtatattgt ggttgtgga 
acgaccgca tcaatttcg attcgttga attgtgata 
taattaaat gagataatc ccaatagtg cctttccta 
tgaacataa tgcgtaact gttagctcg gcaaccctc 
agggatgta gtctttagt tttagtcct gggaacggg 
cactaaagc aaggggacc tgtcgtgcc tggacttga 
ccgacgcct tccttcatt attgttata gtttattaa 
ttagtagaa acaatctca accgttctt gtgttcaat 
gattatcgt acttagtgc tatgcgtgg tgtgtatga 
caaataatg attgtgtac gtacttttt tcacagaaa 
gctaaactc tattgttat atacatcac aagtggatg 
catattata cagtaggta catttacta aaaatgctg 
ttctctcca agcattcgt ccaaagcat ttatagttt 
atgtataag aaattgtat aatttttcc cgatttata 
gtttttact acaaaactg aaatagata aacgtatat 
tttttatat acacaccaa agaattaaa aattcactt 
taagcgaat aaaactata tatttaaac tatcacctc 
tttaaaaaa gtgctctcg ttttatatt ccgtgaatg 
gaacaaagg ccattaaag tagtttttg tagaaaaga 
tcatccgca aaaatactc aactcgccc gaaatttac 
caaattcaa acgtgttgg cttacgact tcatacaaa 
atgtggtac aaacatggt aaagtaata gttgtgtta 
attttgtac atagtagtt tgtaaataa tgtatttac 
aaaataaaa tagtctttt tcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY