| General information | |||
| TC001442 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3885135..3890886 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001442 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03189 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001442 RA
MYSEEQDSH GLLTLHYGK HHATIVDEI KTCFASENF ADMTFVCDD KTTLSAHKL IMAAASPLV RRILGESAH AHGPSVVLI PGIKSCHLR HLLDFLYNG QACVKSSEL DSIQELFEL LQIKSDMWQ SSSTEEAKC QSEHGNSYD NSDLMVVKR ETDDDEKEE GEIKDDDDD TLDENNISE EGGSSPNPV NLSLNTKNT DEEKDIDDD NSSEQLEKK NSDVLKIGQ TTVKIAPKV HELSQFHHH RMKRKSMYI EPIDLKNQE ELKPPIGDQ KLISLVQSP DNYVVTPHR KRRPGFHNS PAQNPPFVP FSPSYIDDA VHHQHHRYK NLPPLIAAH HPLSLSAPP FLVDRIHTP TPGQHSDTV GPPDNVVVK YRPPSADQG VTEAYQNFE HTWGAWSLC QTQVNPSSS NEDSRDPPA SFSVSGGGG GGGSGVSSA GGGDSAESS SHASITTKQ VLPTVQVRE YRCEYCGKQ FGMSWNLKT HLRVHTGEK PFACRLCVA MFKQKAHLL KHLCSVHRN VISSNDGNS GRFNCCFCP LSFESLPEL IRHLSGPHN NLLLSKNMH ELK
- >TC001442 RA
atgtactct gaggaacag gattcccac ggcttatta accttgcat tatggcaaa catcatgca actatagtc gacgaaatt aaaacttgt tttgcctct gagaacttt gccgatatg acattcgta tgtgacgat aaaacaact ttaagcgct cataaatta ataatggcg gcagcgagt cctttagtg cgacgcatt cttggagag agcgcccat gctcacggg cccagcgtt gtgctcatc ccaggaata aaaagctgc cacctccgg cacttgctc gactttttg tacaacggg caagcttgc gttaagtct agcgaattg gacagcatt caggagttg ttcgaactg ttgcaaatt aaatcggac atgtggcag tcttcgtcg accgaagag gccaagtgt cagtccgaa cacggcaac agttacgac aattccgac ttgatggtt gtaaaaaga gaaacggac gacgacgaa aaggaagaa ggcgaaatt aaagacgac gacgatgac actttggac gaaaacaat atttcagaa gaaggcgga tcctcgcca aacccagtg aatctatct ctcaacacg aaaaacacc gacgaggaa aaggacatc gacgatgac aacagctcg gagcagttg gaaaagaaa aattccgac gtgttgaaa atcggccag acgactgtg aaaatcgcc ccgaaagtg cacgaattg tcgcagttc caccaccac cgaatgaaa cgcaaaagt atgtacatt gagccgatc gatttgaag aaccaggaa gaattgaag ccgccgatc ggcgaccag aagctgatc tcgttggtg caaagtccc gacaattac gttgtgact ccgcatcgc aaacggcgt cctggtttc cacaactcc ccggctcaa aacccccca tttgtgccg ttttcgccg tcttacatc gacgacgcc gtgcaccac cagcaccac agatacaag aatttgccg cctttgatc gcagcccac cacccgtta tcgctgtcg gcgccgccg tttctggtg gataggatc cacactccg acgccgggg caacactcc gacaccgtg ggccccccg gacaacgtc gtggtcaag tacaggccg cccagcgcg gaccagggg gtgacggag gcgtaccag aattttgag cacacttgg ggcgcctgg tcgctgtgc cagacgcaa gtgaatccg tcttcgagc aatgaggat tcgagggat ccgccggcg tctttctca gttagtggt ggtggcggt ggtgggggc agtggggtt tcgagtgcc gggggcggt gattcggcg gaaagttcg tcgcatgcg tctatcacc acgaaacaa gtgctgccc acggtgcaa gttcgggag taccggtgt gagtactgt ggcaaacag tttgggatg tcctggaat ttgaagacc cacttgcgg gtgcacacc ggcgaaaaa ccgttcgct tgtcggttg tgtgtcgcg atgttcaag cagaaagca catttattg aaacatctg tgttcagtg catcggaac gttatatcg tcgaacgat ggcaattcc ggacgtttt aactgttgt ttctgcccc ttgtcgttt gagtcccta cccgaattg atcaggcat ttgtcagga ccgcacaat aatttactt ctaagtaaa aacatgcac gagcttaaa taa
- >TC001442 RA
acaaattgt tgttcggac attattata atgttgctt ggtgattat aattatgat ttttacctg agaggatca agtaagtac aaagtgatt tgaggcgag gagagcgga tgttcaaag tttcaagga tgctttgat cctgcagtc tcggctcga cttagcgcg gctctaaca attaccaac catgtgact atcgaccca tatccaatt atgtaaaac ctccaagtt tacgttaat ggtccttaa attttttaa tagggacat tggtgtctg gccgactaa ttgtcccta aagatgttg cgttgcgct gtttttgat gttgtttgt agccgtgtc agttcaacc aagaccact ttcacttcc ctacaaagc ggagacctt gaaggttta aacaggtaa aatcagtct cgaggacag ccgaccaac tacacaaaa agattcatt acttaataa cgccaaact cacatgaac gcgatttgg ggttgtacc ggtggctat tacctatta ttctaagtg ggaatgcga atgaatata ttaggccag agtggttgg caattagct gtgtggatg agactggtt tacggcttg tggccatta ttcattgcg atgatgcga tgtgtcata aagtctttg gaggtcgaa ttctacatt taaggtcca gtttcagac agacattac gcgatagtg aaagtgcta cgctatgcc tactttccc tcgctcaca taacgtaat actaacata acaatagat gcctattac aggaatgcc tggcgcttg aattcggtg gcattttcg ggcagagac tggcacaag gggtggagt gagtgggac aaagcaggc tggtttggc cgggtccgg gagcgcctc cagtggctg aaaattggg caaaagagg aaaaaaagc ataggtctt tctatttct agcggctgc tatttcatc gatattata caaccgttc cccccctcg gctctcggc cgtcggtat tgtgaaagt ccggttgtg gcccctgtt tgggccccg ctcgcgacg agtctcctt gggaagccg gtgaaagca attgaaacg gtctctgca acagtcgaa gtgacgaat gcagaatta caccatccg ctcgagcgg cgtactatc cgtccctaa acgaaaata gcctctatg gcgaaactt cgtgtcact ttgccaaag attcgaaat agtccggga gagcaaaaa tgcgctaaa ttgcgcgca gccgcaagt ggcgtctcc agctttcga ccttgaaaa tgagaaaga gaagttcca agtgagcta gcaatagga aaaaactcg cgatgtagt ggggaggca agagaaaag aggggaaag taaagggag aaattaaga gttagtagt attacgttc gaacccaac ccgtgagca aagccgatg gttctgaat atttcgcag caaagcgca acttttgag ggtggcagt ggtcgcaag cgtcgcgcc gacaccgcc gaaaccgtt ttagggctt gaggaaact tgcttcaat aagcaccgt tgtgttgtg gtgttgtga aggctcgtg ttcgttcac ttttacttt catttgtgg cgcaacaca gtgtttgag agtcgtgtc ccaaaaata taattcact caacgcgaa ggctggcga ccgatcaaa cacggtttt atgtgcttg aacgttcga attatggtg cggtgtgat tattgcaca tcttctggt gctcgcgac tgccctacg gcgaccaca gtcgcgagt gccctttag gactattta ttctctggg ttgtccttg acaaaatta ctaaattcg ccatgtact ctgaggaac aggattccc acggcttat taaccttgc attatggca aacatcatg caactatag tcgacgaaa ttaaaactt gttttgcct ctgagaact ttgccgata tgacattcg tatgtgacg ataaaacaa ctttaagcg ctcataaat taataatgg cggcagcga gtcctttag tgcgacgca ttcttggag agagcgccc atgctcacg ggcccagcg ttgtgctca tcccaggaa taaaaagct gccacctcc ggcacttgc tcgactttt tgtacaacg ggcaagctt gcgttaagt ctagcgaat tggacagca ttcaggagt tgttcgaac tgttgcaaa ttaaatcgg acatgtggc agtcttcgt cgaccgaag aggccaagt gtcagtccg aacacggca acagttacg acaattccg acttgatgg ttgtaaaaa gagaaacgg acgacgacg aaaaggaag aaggcgaaa ttaaagacg acgacgatg acactttgg acgaaaaca atatttcag aagaaggcg gatcctcgc caaacccag tgaatctat ctctcaaca cgaaaaaca ccgacgagg aaaaggaca tcgacgatg acaacagct cggagcagt tggaaaaga aaaattccg acgtgttga aaatcggcc agacgactg tgaaaatcg ccccgaaag tgcacgaat tgtcgcagt tccaccacc accgaatga aacgcaaaa gtatgtaca ttgagccga tcgatttga agaaccagg aagaattga agccgccga tcggcgacc agaagctga tctcgttgg tgcaaagtc ccgacaatt acgttgtga ctccgcatc gcaaacggc gtcctggtt tccacaact ccccggctc aaaaccccc catttgtgc cgttttcgc cgtcttaca tcgacgacg ccgtgcacc accagcacc acagataca agaatttgc cgcctttga tcgcagccc accacccgt tatcgctgt cggcgccgc cgtttctgg tggatagga tccacactc cgacgccgg ggcaacact ccgacaccg tgggccccc cggacaacg tcgtggtca agtacaggc cgcccagcg cggaccagg gggtgacgg aggcgtacc agaattttg agcacactt ggggcgcct ggtcgctgt gccagacgc aagtgaatc cgtcttcga gcaatgagg attcgaggg atccgccgg cgtctttct cagttagtg gtggtggcg gtggtgggg gcagtgggg tttcgagtg ccgggggcg gtgattcgg cggaaagtt cgtcgcatg cgtctatca ccacgaaac aagtgctgc ccacggtgc aagttcggg agtaccggt gtgagtact gtggcaaac agtttggga tgtcctgga atttgaaga cccacttgc gggtgcaca ccggcgaaa aaccgttcg cttgtcggt tgtgtgtcg cgatgttca agcagaaag cacatttat tgaaacatc tgtgttcag tgcatcgga acgttatat cgtcgaacg atggcaatt ccggacgtt ttaactgtt gtttctgcc ccttgtcgt ttgagtccc tacccgaat tgatcaggc atttgtcag gaccgcaca ataatttac ttctaagta aaaacatgc acgagctta aataacatt ccatgattg catgaggtc gtatattgt ggttgtgga acgaccgca tcaatttcg attcgttga attgtgata taattaaat gagataatc ccaatagtg cctttccta tgaacataa tgcgtaact gttagctcg gcaaccctc agggatgta gtctttagt tttagtcct gggaacggg cactaaagc aaggggacc tgtcgtgcc tggacttga ccgacgcct tccttcatt attgttata gtttattaa ttagtagaa acaatctca accgttctt gtgttcaat gattatcgt acttagtgc tatgcgtgg tgtgtatga caaataatg attgtgtac gtacttttt tcacagaaa gctaaactc tattgttat atacatcac aagtggatg catattata cagtaggta catttacta aaaatgctg ttctctcca agcattcgt ccaaagcat ttatagttt atgtataag aaattgtat aatttttcc cgatttata gtttttact acaaaactg aaatagata aacgtatat tttttatat acacaccaa agaattaaa aattcactt taagcgaat aaaactata tatttaaac tatcacctc tttaaaaaa gtgctctcg ttttatatt ccgtgaatg gaacaaagg ccattaaag tagtttttg tagaaaaga tcatccgca aaaatactc aactcgccc gaaatttac caaattcaa acgtgttgg cttacgact tcatacaaa atgtggtac aaacatggt aaagtaata gttgtgtta attttgtac atagtagtt tgtaaataa tgtatttac aaaataaaa tagtctttt tcta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
