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Gene information
General information
TC001434 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3952709..3958496
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001434
    iBeetle screen iB_03186

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001434 RA
      MCESRVNEV STIVRNIKY DRHVYDSEN VIEKENPYY 
      SKKYEEAYK KSITNPEEF WPEVGKLVT WTKPWDKVM 
      DHSHPPFTK WFAGGELNA CYNAVDRHV EAGRGSKVA 
      LIHDSPLTK TVRKITYAE LLDQVSHLA GALAKLGVS 
      KGDRVLIYM PLIPEAVIA MLATARLGG VHSVVFGGF 
      AARELCARI VHAEPKVII AASCGIEPH KIVRYKDIL 
      NDAIEWSSH KPNKCIIFQ RRNVECAPI DVEMDILWE 
      DALKMAGPH ECIPVEAND PLYILYTSG TTDQPKGIQ 
      RPVGGHIAT LTWSMSVIY GMGPNDVWW AASDLGWVV 
      GHSYICYAP LLYGITSVM YEGKPDRTP DPGQYFRII 
      NEHKVNAIF TVPTAFRVI HREDPDIEF GRKYSIRSL 
      RTIFVAGEH CDYETKMWA EKTFNVPVL NHWWQTETG 
      HAITATCLG YGHNLSPPK YTAGMPFPG YDIKILRSD 
      GMEASNNEL GRIVIKLPL PPGTMSTLY QAPERFCQV 
      YFSRYPGYY DTMDAGYKD EYGYIYVTA RDDDVINVA 
      GHRISTSAL EDVVLAHPD IADATVVGV PEHTKGEVP 
      LCLYVIKRN AKKSEVAIS RDLLRMVRE LVGPIAAFR 
      MAVAVRGLP RTRSGKTCR KSISDLARN KEVRISGTV 
      EDPTVYKDI KAALQKLGF AHTAPDPQ
    CDS sequence
    • >TC001434 RA
      atgtgtgaa tcaagagtc aacgaagtt agcaccatt 
      gtgaggaat atcaaatac gaccggcac gtgtacgac 
      tccgaaaac gtaatcgag aaggaaaac ccttactat 
      agcaaaaag tacgaagag gcctacaag aagtccatc 
      accaacccc gaggagttc tggcccgag gtgggcaag 
      ctggtcaca tggaccaag ccctgggac aaggtgatg 
      gaccactcc catcccccg ttcaccaaa tggttcgca 
      gggggcgaa ctcaacgcc tgctacaac gccgtggac 
      cggcacgtg gaggcgggc cggggctcc aaggtggcc 
      ctcatccac gacagcccc ctgaccaaa accgtgcgg 
      aaaatcacc tacgccgag ctgctcgac caagtctcg 
      catctggcg ggcgcgctg gccaagctg ggggtgtcc 
      aagggggac cgcgtgctc atttacatg ccgctcatc 
      cccgaagcg gtcatcgcg atgctggcg acggcacgg 
      ctcggggga gtgcactcg gtggttttc ggaggtttt 
      gcggcgaga gagctttgc gctcggatt gtgcacgcg 
      gagcccaaa gtgataatc gctgcgagt tgtgggata 
      gaaccgcat aaaatcgta cgatataag gatatcctg 
      aacgatgca attgagtgg tcgtcccac aagcccaac 
      aagtgtatt atcttccaa agacggaac gtggagtgt 
      gcccccata gacgtcgaa atggatatt ctgtgggag 
      gatgcgctg aaaatggcc ggacctcac gagtgcatc 
      ccggttgag gccaacgat ccgctctat attctctat 
      acgtctgga accacagac cagccgaag ggcatccag 
      aggccggtc ggcggccac atcgcaacc ctcacttgg 
      agtatgagc gtgatctac ggaatgggc ccgaacgac 
      gtttggtgg gcggccagc gatctgggc tgggttgtt 
      ggtcattcg tacatttgt tacgcgccg cttttgtat 
      ggaataaca agcgtaatg tacgaggga aaacccgac 
      cggacgcca gacccaggc caatacttt aggataatt 
      aacgagcac aaagttaat gcaatcttt actgttcct 
      actgctttc cgggtgata catcgcgag gaccccgat 
      attgagttc gggcgaaaa tattcgatt aggagttta 
      cggacgatt ttcgtggct ggggagcac tgcgactat 
      gaaacgaaa atgtgggcc gaaaagacg ttcaatgtg 
      cccgttttg aaccactgg tggcagacg gagactggg 
      catgccatc actgccaca tgtctgggg tatgggcat 
      aatctgagt ccgccgaag tacacggca gggatgcct 
      tttccggga tacgatatt aagattttg cggtcggac 
      ggcatggaa gcgtcaaat aacgaattg ggccgaatt 
      gtgataaag ctgccgctg ccgccgggg accatgtcc 
      acgttgtac caagcgccc gaacggttt tgccaagtc 
      tatttcagc cgataccca ggctattat gacaccatg 
      gacgcgggg tacaaggac gagtacggt tacatttat 
      gtgacggcc cgtgacgac gatgtgatt aatgtcgct 
      gggcacaga atatccact tcggcgttg gaggacgtg 
      gttttggcc cacccagac attgccgac gcgacggtg 
      gtcggagtt ccggagcat acgaaaggg gaggttccg 
      ctctgttta tatgtcata aagagaaat gccaaaaag 
      agtgaagtc gcaataagt cgcgattta ttaaggatg 
      gttagggaa ttggtcgga ccaatcgca gcgtttcgc 
      atggcggtg gcagtgcga ggtttgccg cgaactcgc 
      tcgggtaaa acgtgcagg aaatctata tcggattta 
      gcccgaaat aaggaagtt aggatttcg ggcactgta 
      gaagatcca acagtttac aaagatatt aaggctgct 
      ctccaaaaa ctggggttc gcacatact gcgccggat 
      ccccagtga
    mRNA sequence
    • >TC001434 RA
      ttaaaaacg attgtttaa taaagcgtg cctagtggt 
      gtgaagttt ccatagtat gttaaatcc gaaagaaca 
      gaatacgat gtgtgaatc aagagtcaa cgaagttag 
      caccattgt gaggaatat caaatacga ccggcacgt 
      gtacgactc cgaaaacgt aatcgagaa ggaaaaccc 
      ttactatag caaaaagta cgaagaggc ctacaagaa 
      gtccatcac caaccccga ggagttctg gcccgaggt 
      gggcaagct ggtcacatg gaccaagcc ctgggacaa 
      ggtgatgga ccactccca tcccccgtt caccaaatg 
      gttcgcagg gggcgaact caacgcctg ctacaacgc 
      cgtggaccg gcacgtgga ggcgggccg gggctccaa 
      ggtggccct catccacga cagccccct gaccaaaac 
      cgtgcggaa aatcaccta cgccgagct gctcgacca 
      agtctcgca tctggcggg cgcgctggc caagctggg 
      ggtgtccaa gggggaccg cgtgctcat ttacatgcc 
      gctcatccc cgaagcggt catcgcgat gctggcgac 
      ggcacggct cgggggagt gcactcggt ggttttcgg 
      aggttttgc ggcgagaga gctttgcgc tcggattgt 
      gcacgcgga gcccaaagt gataatcgc tgcgagttg 
      tgggataga accgcataa aatcgtacg atataagga 
      tatcctgaa cgatgcaat tgagtggtc gtcccacaa 
      gcccaacaa gtgtattat cttccaaag acggaacgt 
      ggagtgtgc ccccataga cgtcgaaat ggatattct 
      gtgggagga tgcgctgaa aatggccgg acctcacga 
      gtgcatccc ggttgaggc caacgatcc gctctatat 
      tctctatac gtctggaac cacagacca gccgaaggg 
      catccagag gccggtcgg cggccacat cgcaaccct 
      cacttggag tatgagcgt gatctacgg aatgggccc 
      gaacgacgt ttggtgggc ggccagcga tctgggctg 
      ggttgttgg tcattcgta catttgtta cgcgccgct 
      tttgtatgg aataacaag cgtaatgta cgagggaaa 
      acccgaccg gacgccaga cccaggcca atactttag 
      gataattaa cgagcacaa agttaatgc aatctttac 
      tgttcctac tgctttccg ggtgataca tcgcgagga 
      ccccgatat tgagttcgg gcgaaaata ttcgattag 
      gagtttacg gacgatttt cgtggctgg ggagcactg 
      cgactatga aacgaaaat gtgggccga aaagacgtt 
      caatgtgcc cgttttgaa ccactggtg gcagacgga 
      gactgggca tgccatcac tgccacatg tctggggta 
      tgggcataa tctgagtcc gccgaagta cacggcagg 
      gatgccttt tccgggata cgatattaa gattttgcg 
      gtcggacgg catggaagc gtcaaataa cgaattggg 
      ccgaattgt gataaagct gccgctgcc gccggggac 
      catgtccac gttgtacca agcgcccga acggttttg 
      ccaagtcta tttcagccg atacccagg ctattatga 
      caccatgga cgcggggta caaggacga gtacggtta 
      catttatgt gacggcccg tgacgacga tgtgattaa 
      tgtcgctgg gcacagaat atccacttc ggcgttgga 
      ggacgtggt tttggccca cccagacat tgccgacgc 
      gacggtggt cggagttcc ggagcatac gaaagggga 
      ggttccgct ctgtttata tgtcataaa gagaaatgc 
      caaaaagag tgaagtcgc aataagtcg cgatttatt 
      aaggatggt tagggaatt ggtcggacc aatcgcagc 
      gtttcgcat ggcggtggc agtgcgagg tttgccgcg 
      aactcgctc gggtaaaac gtgcaggaa atctatatc 
      ggatttagc ccgaaataa ggaagttag gatttcggg 
      cactgtaga agatccaac agtttacaa agatattaa 
      ggctgctct ccaaaaact ggggttcgc acatactgc 
      gccggatcc ccagtgaaa actgtttac aatttaaat 
      aagtataga acatttcca ttagtttat gttacattc 
      gagtttatt agtattttg taacttatt acctacttg 
      ttttattta aattgttgg tagtttctt ttgctctta 
      atcagatac aaattaatg attgtgtta tgtagatag 
      ataaggaac gaaaatagt aagagaatt gtatcaatt 
      tgaccgagt gacggaacg aaagacaaa tggcaaaat 
      cgcgagtgt gaatcaaaa tatatatag agaggtgag 
      gttgttaaa taaaattca gtgttaatt atatatgcg 
      tgattatat tttaaaatt catgtttaa tatgaaaat 
      attcatagc attttgtaa attctttaa attatatta 
      caacgttga agaacaaac attcatatg aacttgaaa 
      aatatctga cagaacaaa tgtaataaa aaaggcctt 
      tcacaacaa taaaataat ttacaggcg atcctgaaa 
      tggaatcat taaaacact ttaacagca tgttattca 
      cctctcttt aatttaaag ctcaaaata cctttatca 
      tataaataa aagcacaga cagacaata aacttaatt 
      aggtagaat ggtcacggc cgtactccg atccacctt 
      tagttcgat cctgagcca ccggaccag ccagtggcg 
      cgagtatgc gcgaatagc gcgagaggc gagttcgta 
      taaaataac aaccataac atgataacg atttaattg 
      taccattat aaattacaa ataacaaca ttcaactta 
      aaaatgcca cgtttgaca aaattcggc ataatcggg 
      acttatcat atcttatat caatcaata tctgcacat 
      ctggtctgc tactgcttc cggcctgaa tcggcctca 
      actaacacc ccttcgctt tatcaataa aatttactt 
      taagtgtac aactacgaa aacaaacga ggtaatgat 
      tggtaaatg attgcggtt cgtttgatc aactagaag 
      tgtatggag tctgatggg caagggcgt gacgaacca 
      cagatagat attaattat aacataagt aaattatat 
      tacatagta ataggaacg cgcactacc acgactcta 
      tcgtgagag ggcacgtcc ggtccgcaa cgacttcac 
      ttccaagat gcgaccacc atcgccacc tttcattat 
      ttctctgct gactagaat atgctgcga caagtcaaa 
      acttggaaa tttagccga cgagatgtt cgttcgttc 
      gttctaagt cacaaaaat gacgcgatt tgcgctcgg 
      acggcggcg aaggtgcgc aattcgccc agaccgcac 
      gatgccaca aatcgggcc ccatttttg cctagggtc 
      tgcgaattc ccacgaaca tcttaaccg atggatccg 
      atacggagt agtgtataa atgtgtgcg cagcgcgcc 
      ggcggccgc gaaccaagg cccaaacgt aacaatatc 
      aaacgacac gcatacgcc ttgcatttt acccgatgt 
      tcaatcagt ttttcaata aaatgctaa aaatactcg 
      ctaagtgct aaggccgaa gtactaagt accgtcacc 
      gactagaag ggtgcttgg tcagggctc gaggacaga 
      ccgacgcgc cattataca cagctgcta actctacgt 
      acgtaataa ataacttta gagtactgt actgtttgt 
      ttgttccag tttagttgt tcgaaattt cgtgaatta 
      aaatacatt tagataaag tataaaaga aatgaatct 
      cggaggtcg aggctgccc ctcgtacag tatcatcat 
      cttaaatat gtatttatg tatatctag tgttcaccg 
      ctagactac ctacgtaat aactaagtc agagttaga 
      cctagatta caacagaag aaggcgagg agaagcagt 
      aattcgact aacgatata actatatat ttataaaga 
      ctaaaatct ataactgcg tgtggttat tgcctcccc 
      tccaacatt tggcagaca acatctgat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY