Status
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Gene information
General information
TC001431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3977503..3982736
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001431
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03184

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001431 RA
      MDSGMQQCQ FYCGPRALT LFVLLHICL SLPNESLAG 
      YNEKRLLHK LLDHYNVLE RPVANESDP LQLSFGLTL 
      MQIIDVDEK NQLLVTNIW LKLEWNDMN LRWNASEFG 
      GVKDLRIPP HRLWKPDVL MYNSADEGF DGTYPTNVV 
      VRNNGSCLY VPPGIFKST CKIDITWFP FDDQRCEMK 
      FGSWTYDGL QLDLQLQDD AGGDISSFI TNGEWDLLG 
      VPGKRNEIY YNCCPEPYI DITFVIIIR RRTLYYFFN 
      LIVPCVLIA SMAVLGFTL PPDSGEKLS LGVTILLSL 
      TVFLNMVAE TMPATSDAV PLLGTYFNC IMFMVASSV 
      VSTILILNY HHRNADTHE MSEWIKVVF LIWLPWLLR 
      MHRPHGSCE YGQQPSRPA SVATDRKPL HFQDVELKE 
      RSSKSLLAN VLDIDDDFR HNHRGGGTP TPLPTATFF 
      RTVYRQNED NGNGGRLHE SLVSNHSCL SADYELALI 
      LKEIRFITD QLRKEDEAA DITRDWKFA AMVVDRLCL 
      IIFTLFTII ATLAVLFSA PHIIVS
    CDS sequence
    • >TC001431 RA
      atggacagt ggcatgcag caatgtcaa ttctactgc 
      ggtccacga gcactgacg ctctttgtc ctcttgcac 
      atctgcctg tcgctaccg aacgaatct ttggcggga 
      tacaacgag aagcgactc cttcacaaa ttgcttgac 
      cattacaac gtactggaa cggccggtt gccaacgag 
      tcggaccct ttgcaactc agtttcggt ttaacttta 
      atgcaaatc atcgatgtg gacgaaaaa aaccagctg 
      ctcgtcacc aacatttgg ctaaagttg gaatggaat 
      gacatgaac ttgcgatgg aatgcatcc gagtttggc 
      ggcgtcaag gacctccgc atacctcca cataggctg 
      tggaagccg gatgttctt atgtacaac agcgccgat 
      gagggcttt gacgggacg taccccacg aacgtggtc 
      gtacgaaac aacggcagc tgcttgtac gtgcctccc 
      ggaattttt aaaagcact tgtaagatt gacatcacg 
      tggtttccg ttcgacgac cagcgatgc gaaatgaaa 
      tttggaagt tggacttat gacggatta caactcgat 
      ctacaatta caagacgat gcgggaggg gatatcagc 
      agctttata acaaacggc gaatgggat ttgctagga 
      gttcctggc aaacggaac gaaatctac tataattgc 
      tgccctgag ccgtacatc gatatcaca ttcgtcatc 
      attattcga cggcggaca ctctactac ttttttaac 
      ttgatcgta ccgtgcgtg cttattgct tccatggcg 
      gtattaggg ttcactctg cctccggac tccggagag 
      aaactctca ctaggagtc acgatttta ctatctctg 
      acggtgttt ttaaacatg gttgccgaa acgatgcct 
      gctacctca gatgcagtt ccactgctg ggcacctac 
      ttcaactgc atcatgttc atggtggca tcgtcggtt 
      gtctcaacc attcttatt ctcaattat caccatcgc 
      aacgctgac acgcacgaa atgtcagaa tggataaag 
      gtcgttttc ctgatttgg ttaccatgg cttctacgt 
      atgcacaga ccgcacggc agttgtgag tacgggcaa 
      cagccgagt cgacccgca tctgtggca acagatcgc 
      aaaccgctg cactttcaa gacgtggag ttgaaagag 
      cgttcttcc aaaagtttg cttgcgaac gtgttagac 
      atagacgac gattttaga cacaaccat cgcggcggc 
      gggactccg acgcccctc ccgacagcc accttcttc 
      cgaacagtc tacaggcaa aacgaagac aacggaaac 
      ggaggccga ctccacgag tcgctcgtg tcgaaccac 
      tcttgtcta agtgcagat tacgagctg gcgctgatt 
      ctgaaggag atccgcttc atcaccgac cagctgcgt 
      aaggaagac gaggccgcg gacatcacc agagattgg 
      aaattcgcg gccatggtg gtcgacaga ctgtgcctt 
      attatattc actctgttc acgatcatc gccacgtta 
      gcagtgctg ttctcggca cctcacatc atcgtatcg 
      tag
    mRNA sequence
    • >TC001431 RA
      gccaacaat tgaataaat ttcaagagg aatcatccc 
      tgctggtct ggttcgggt cgggttcgg gcagtgggc 
      tcgcggtag cgcagtagc actttggcg gaggagcat 
      ccgactcgc aatggcttt agtatacga atgtgcaaa 
      gtaggcacg agatagtaa acatgaagt aactaaatc 
      taaaccatc tgtcggtaa aactaaact atatgtata 
      tacatgtgt atgtatagt atcgccagt actgtgata 
      cataggtat atatgtata aacgctaaa cttaaactc 
      atgaaccga acatttaaa caattgtgt actaataaa 
      taatgcttt aagctaaac ccgactaaa taataaacg 
      attctaaca tgaaaccgc aataaacca aaaactaaa 
      ctcaactct aaacgatcg ttggaacac agcactggc 
      gcgagctct ttactgcta atatcgcta cccatggac 
      agtggcatg cagcaatgt caattctac tgcggtcca 
      cgagcactg acgctcttt gtcctcttg cacatctgc 
      ctgtcgcta ccgaacgaa tctttggcg ggatacaac 
      gagaagcga ctccttcac aaattgctt gaccattac 
      aacgtactg gaacggccg gttgccaac gagtcggac 
      cctttgcaa ctcagtttc ggtttaact ttaatgcaa 
      atcatcgat gtggacgaa aaaaaccag ctgctcgtc 
      accaacatt tggctaaag ttggaatgg aatgacatg 
      aacttgcga tggaatgca tccgagttt ggcggcgtc 
      aaggacctc cgcatacct ccacatagg ctgtggaag 
      ccggatgtt cttatgtac aacagcgcc gatgagggc 
      tttgacggg acgtacccc acgaacgtg gtcgtacga 
      aacaacggc agctgcttg tacgtgcct cccggaatt 
      tttaaaagc acttgtaag attgacatc acgtggttt 
      ccgttcgac gaccagcga tgcgaaatg aaatttgga 
      agttggact tatgacgga ttacaactc gatctacaa 
      ttacaagac gatgcggga ggggatatc agcagcttt 
      ataacaaac ggcgaatgg gatttgcta ggagttcct 
      ggcaaacgg aacgaaatc tactataat tgctgccct 
      gagccgtac atcgatatc acattcgtc atcattatt 
      cgacggcgg acactctac tactttttt aacttgatc 
      gtaccgtgc gtgcttatt gcttccatg gcggtatta 
      gggttcact ctgcctccg gactccgga gagaaactc 
      tcactagga gtcacgatt ttactatct ctgacggtg 
      tttttaaac atggttgcc gaaacgatg cctgctacc 
      tcagatgca gttccactg ctgggcacc tacttcaac 
      tgcatcatg ttcatggtg gcatcgtcg gttgtctca 
      accattctt attctcaat tatcaccat cgcaacgct 
      gacacgcac gaaatgtca gaatggata aaggtcgtt 
      ttcctgatt tggttacca tggcttcta cgtatgcac 
      agaccgcac ggcagttgt gagtacggg caacagccg 
      agtcgaccc gcatctgtg gcaacagat cgcaaaccg 
      ctgcacttt caagacgtg gagttgaaa gagcgttct 
      tccaaaagt ttgcttgcg aacgtgtta gacatagac 
      gacgatttt agacacaac catcgcggc ggcgggact 
      ccgacgccc ctcccgaca gccaccttc ttccgaaca 
      gtctacagg caaaacgaa gacaacgga aacggaggc 
      cgactccac gagtcgctc gtgtcgaac cactcttgt 
      ctaagtgca gattacgag ctggcgctg attctgaag 
      gagatccgc ttcatcacc gaccagctg cgtaaggaa 
      gacgaggcc gcggacatc accagagat tggaaattc 
      gcggccatg gtggtcgac agactgtgc cttattata 
      ttcactctg ttcacgatc atcgccacg ttagcagtg 
      ctgttctcg gcacctcac atcatcgta tcgtagcta 
      ccccgaccc cggctcctc gcaagaccc tccccgaag 
      ctagatcct gctgatgat ttcgggctc ggatcggga 
      tccgaatcg atccccgcg ctcgcgcca ccaaagcac 
      ccatcaata gttggcagt cgttttata cggaaattt 
      ggttttgta tcgtgttct ttagggtat taacacaat 
      catactgtg ataaatcaa gttaaatgt tgagttagt 
      cagacgaga tcgtgaatt ggcataggc agagcggag 
      cgacgagcg tttcacttt cctcggact atgtgataa 
      gaggacagg ccaatggag attggagac tcacaattc 
      gtctcttgt tcgcctgtt tcgcactcc gaaagaacc 
      cgtgccatt tgcgaaccc gacaatagt aacgtgcaa 
      tgttttgtc cgcgaagag agggacacg attgtttct 
      gtcgctgtt ccccacttc gtattgtta cttgacaca 
      ttgttaatg gcgttttat attgtatga cgtgtagaa 
      ctatatttt tctcgtgga atttgaaat aatttaatt 
      aataattat taaaaaatt cattactaa aatttataa 
      aagaaacaa aacgaaacg ttggtatga ttagataat 
      tcggatgaa gtcttagaa accgcttca tcttttttt 
      atattttgt ccgtctagc atgctacgt aggatgtaa 
      ctgtcatct atggacttc tctatcctg tacatacag 
      aacctaact taggagcat agctggtga aaaagcact 
      tgaaattct aacatatcc tttcaaagt catgtaacg 
      tacagagtc gtgtataaa aaatactca tgtctctag 
      tgggaaaac gtcgtgcgt gtttccggt gtcgcaaaa 
      accacgctt ttcattgaa aatacattt tctcgaatt 
      atttgtaac cagacaaaa aaaattcca tgcaatatc 
      cggactctt cgtcgaatg aaggaagtt gctcaaata 
      atattaaaa cgccgaaaa ttgacatcg actcttaat 
      cgagtccgg gagtccgga taaaaccgc gcgctggag 
      aaacaaaca aatcgcaac aatacatcg ccaacgttc 
      ttccactgt caagcaaga caatatctc gtcagttag 
      ggctataat tcttattct tagtcttta tttacatct 
      taatatagc aaaagaatg cggtaaata ccgaaacca 
      ttgcgagtg cgagtcctt atccgttta tattttagg 
      ttttaggca aattaatga atgacgaat ggcagtttg 
      attgttatc gctgattac gcaacggcg gaaacgctt 
      agcgtcatc tatctctct taatggctt aaagacaac 
      ggaatggtg tcttcggat ggtggccac catcggggc 
      cactgtacg aaagcacaa gacagcgtg caaatctct 
      ttcgatgat atacataat tataagtaa taaatctcg 
      cgttccatt gttatataa ataagtact tcccagaga 
      actattatt cactaaaaa tgctaatcg gattaaaca 
      ctattttaa agaaactcg acaatccat tattggtcg 
      ctaattaac gacacgaga gggcctttg ggacagcag 
      aagaccggc tgtcggttg agcttgcga gcggcgctc 
      gcaaactca atcgcatca aagcgacaa gtactgttt 
      tctaccgaa attgttgta aattgtatc acaatggat 
      tgtactttg ttaaatgaa tttcaatta aacttgcaa 
      atgcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY