Status
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Gene information
General information
TC031838
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001430
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451216.1: 24..2666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031838
      iBeetle screen iB_03183

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031838 RA
        MEEPLEIEG WKRISILGS GAFGVVALW QHAGSGDYI 
        AIKKCKFRT SGHLSDRQK QRWENEVQI MKTLNCPNI 
        VSFKLLPPH LEAVLLKYN PTRLPLLSM EYCRKGNLR 
        HVLNQAKNS SGLGESDVR TVLADVTQA VSYLHKQNI 
        THRDIKPEN IVLQHCNTR PGDVIYKLI DLGYAKELS 
        DSVVSFVGT LHYLAPEIM KTENYDFSV DYWSLGVVA 
        FEVICGVLP FLPLYTPME RFSFIERKK PEHICIYQL 
        HSGSVMYSS ELLKENHIS TCLKQNIES WLRHVLQFH 
        PTQRRANFP DNSNVFDCL LNILDKKIV TVFSVHTLE 
        FYSYEINES ILISTLKDW IARDTKVQK SDQILLSSL 
        GFFDIKDDK YVVDILAKV RLVTTSGAL
      CDS sequence
      • >TC031838 RA
        atggaggag cccctcgag atcgagggc tggaagcgc 
        atctcgatc ctcggttcc ggggccttt ggggtggtc 
        gccctgtgg cagcacgcc ggttcgggc gattacatc 
        gctataaaa aagtgcaaa ttccggact agcggtcac 
        ctcagtgac cggcagaag cagcgatgg gagaacgaa 
        gtgcaaatt atgaaaact ctaaactgt ccaaatatt 
        gtttcgttt aagctgttg cccccacat ttggaggct 
        gttttgctc aagtataac ccgacaaga ctgccgctg 
        ttgtccatg gagtattgc aggaaggga aacctgaga 
        catgtactt aatcaagcg aaaaactca tcagggttg 
        ggggaaagt gacgtcagg actgtctta gctgatgtt 
        actcaagcc gtttcttac ttacataaa cagaatatt 
        acgcacaga gatatcaaa ccggaaaat attgttttg 
        caacattgt aacactaga cctggagac gttatttac 
        aagttgatt gatttgggg tatgccaag gaattgagc 
        gattctgtt gtcagtttc gtgggcacg ttgcattat 
        ctcgcgcct gaaatcatg aaaacggaa aactatgac 
        ttcagtgtt gattattgg tcgttgggg gtcgtggcc 
        tttgaagtc atttgcggg gttttaccc tttttgccc 
        ctatatacg ccaatggaa agattctca tttattgaa 
        cgaaaaaaa ccggaacac atttgcatc taccagtta 
        cattcaggc agcgttatg tattcctcc gaattgttg 
        aaagaaaac cacatttcc acgtgcctt aaacaaaac 
        atcgaaagt tggctcaga cacgtccta cagttccac 
        ccaacgcaa cgtcgggcc aatttccct gacaactcc 
        aacgtcttc gactgtttg ctcaatatt ttagataag 
        aaaatcgtc accgtattc tcggtccac acgttagag 
        ttctattct tacgaaatc aacgaaagt attttaata 
        agcaccctg aaagactgg atagcgcgt gatacaaaa 
        gtgcaaaag agtgatcaa attctactg agtagctta 
        ggttttttt gacattaaa gacgacaag tacgtcgtg 
        gatattctc gcaaaggtg aggctggtc accacaagt 
        ggcgccctc tag
      mRNA sequence
      • >TC031838 RA
        agagtatat aatcgttat tgttgccct gtcaagagc 
        ccagataaa tggccacac ggtcacgta cttccatcg 
        ctcatattt tgcgcgatt tcaatgcaa acagtcact 
        tactcacgt tttgcgacc aaagtggtt acgatttcc 
        cggaaaaat gtagcaaaa tctcgcgta aacctccgt 
        ctagttatg tgcctgtta ggttagtgt agtaattgc 
        agcgagctt tattccaac tcacccgat taaaaccgt 
        gagtttggg tgagttttg ctataaatt cgtttgagg 
        ttaggtcag gatggagga gcccctcga gatcgaggg 
        ctggaagcg catctcgat cctcggttc cggggcctt 
        tggggtggt cgccctgtg gcagcacgc cggttcggg 
        cgattacat cgctataaa aaagtgcaa attccggac 
        tagcggtca cctcagtga ccggcagaa gcagcgatg 
        ggagaacga agtgcaaat tatgaaaac tctaaactg 
        tccaaatat tgtttcgtt taagctgtt gcccccaca 
        tttggaggc tgttttgct caagtataa cccgacaag 
        actgccgct gttgtccat ggagtattg caggaaggg 
        aaacctgag acatgtact taatcaagc gaaaaactc 
        atcagggtt gggggaaag tgacgtcag gactgtctt 
        agctgatgt tactcaagc cgtttctta cttacataa 
        acagaatat tacgcacag agatatcaa accggaaaa 
        tattgtttt gcaacattg taacactag acctggaga 
        cgttattta caagttgat tgatttggg gtatgccaa 
        ggaattgag cgattctgt tgtcagttt cgtgggcac 
        gttgcatta tctcgcgcc tgaaatcat gaaaacgga 
        aaactatga cttcagtgt tgattattg gtcgttggg 
        ggtcgtggc ctttgaagt catttgcgg ggttttacc 
        ctttttgcc cctatatac gccaatgga aagattctc 
        atttattga acgaaaaaa accggaaca catttgcat 
        ctaccagtt acattcagg cagcgttat gtattcctc 
        cgaattgtt gaaagaaaa ccacatttc cacgtgcct 
        taaacaaaa catcgaaag ttggctcag acacgtcct 
        acagttcca cccaacgca acgtcgggc caatttccc 
        tgacaactc caacgtctt cgactgttt gctcaatat 
        tttagataa gaaaatcgt caccgtatt ctcggtcca 
        cacgttaga gttctattc ttacgaaat caacgaaag 
        tattttaat aagcaccct gaaagactg gatagcgcg 
        tgatacaaa agtgcaaaa gagtgatca aattctact 
        gagtagctt aggtttttt tgacattaa agacgacaa 
        gtacgtcgt ggatattct cgcaaaggt gaggctggt 
        caccacaag tggcgccct ctagcggta tttttagga 
        cgatctgga gctgtttgt gttcaagaa aggcgcttt 
        catcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY