| General information | |||
| TC001429 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450265.1: 217..10203 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001429 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03182 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001429 RA
MEQVATFMG HTKKTHAEF YRLPQDIYQ TAKVAKILL LLEKGKGEQ FKGKSLNEI ELERDIYYS SESESETDE AIPLAEKVL RKAANAPLQ NKEEKKNEN AENNTNTVT KQLNLEEEV CSSNMEQEA DAEVEDVNG NSKKTENTN KSIGKKKEK ENNSEMEVD TNNNRKNAG GKNKPLGKK SESSNSVRH RWSEKEKRI VLHHFRGHI LKKVAPKKH ECEELISQH NDILGCKSD DNEQGLDEG GNKSPTINI QSVVTFRDG IRVDKTEGK RNDTKEEES VLPNDENQE LFLADSELT IYSANEYSN SKVQLSKRK QRHMSEVSI QQLEKMPKG SIERKQFIA MLRKKGNFL SPAQNEIKV VKRPVKEVD PSNILPCVY CKGFYMKRY LKRHVKTCL QRPTTSGAE DFRTADAQS YLAFRSVDQ KFIDELRFK EGILNMRCD NIAFIAKSD PLILSFAAF YAKRHRHNH LNKVTKNKI RELARLWKD MKKYINDKN FFAALKPEN FKYFVLSTQ NITGYNHNS QKFEKAPSL ALHMGTTLR KVCEFAFSE IQQKNHIFI SGLDILKKT KDIKVLKEL IKNNWSAEV SSVANQNLV ENQWNKPTI IPLTSDIKK LNNYVKLRA EQCADKLKK QEDDKKSFN DLQKCCFTL LITLNRRRI GELERLELE SYLKKNNDL ISEEFEQKL TETEKFLLK KYKRIRIRG KRHKPVPVL ISKEVQEYI NLLLLVRKN FVPENGYAA LRWLAQSSG AEYPKTLTS GKLRKHIAT ISQISDLSS SELEQLCTF LGHTMTTHQ DFYRLPQEI YQTAKLTKL LLKSQNESV DVDDMNQEL GSDGDNDQS DDDEVINVV PSTSNGSGE GIGQNLKEK IITNSINVD KRSEQKSNG KGKSKKASR YNLDENQLK ISVMIRKPP TESEIEEFS KKYPIMENK DWKKIKVFV YNEYSKK
- >TC001429 RB
MEQVATFMG HTKKTHAEF YRLPQDIYQ TAKVAKILL LLEKGKGEQ FKGKSLNEI ELERDIYYS SESESETDE AIPLAEKVL RKAANAPLQ NKEEKKNEN AENNTNTVT KQLNLEEEV CSSNMEQEA DAEVEDVNG NSKKTENTN KSIGKKKEK ENNSEMEVD TNNNRKNAG GKNKPLGKK SESSNSVRH RWSEKEKRI VLHHFRGHI LKKVAPKKH ECEELISQH NDILGCKSD DNEQGLDEG GNKSPTINI QSVVTFRDG IRVDKTEGK RNDTKEEES VLPNDENQE LFLADSELT IYSANEYSN SKVQLSKRK QRHMSEVSI QQLEKMPKG SIERKQFIA MLRKKGNFL SPAQNEIKV VKRPVKEVD PSNILPCVY CKGFYMKRY LKRHVKTCL QRPTTSGAE DFRTADAQS YLAFRSVDQ KFIDELRFK EGILNMRCD NIAFIAKSD PLILSFAAF YAKRHRHNH LNKVTKNKI RELARLWKD MKKYINDKN FFAALKPEN FKYFVLSTQ NITGYNHNS QKFEKAPSL ALHMGTTLR KVCEFAFSE IQQKNHIFI SGLDILKKT KDIKVLKEL IKNNWSAEV SSVANQNLV ENQWNKPTI IPLTSDIKK LNNYVKLRA EQCADKLKK QEDDKKSFN DLQKCCFTL LITLNRRRI GELERLELE SYLKKNNDL ISEEFEQKL TETEKFLLK KYKRIRIRG KRHKPVPVL ISKEVQEYI NLLLLVRKN FVPENGYAA LRWLAQSSG AEYPKTLTS GKLRKHIAT ISQISDLSS SELEQLCTF LGHTMTTHQ DFYRLPQEI YQTAKLTKL LLKSQNESV DVDDMNQEL GSDGDNDQS DDDEVINVV PSTSEGIGQ NLKEKIITN SINVDKRSE QKSNGKGKS KKASRYNLD ENQLKISVM IRKPPTESE IEEFSKKYP IMENKDWKK IKVFVYNEY SKK
- >TC001429 RC
MFKAPSLAL HMGTNLKIL CDVALKVVI EKRHLPNLR WEDDREKKT EIKELKKLI SGHWCNELS SLALKNLKE KHWEKPTEL PLTSDILTL QTFISEQAD KAYNNLINE GKTNMRQNY KTLAQCVLA LTIMFNRKR VGDVQYLKL ATYISADKN SVIEGEQAF MRSLTSVEQ ILSKRFKRV VTGGKGSKP VPILFSIKL QKIIDCLIK IRTETSIVP TSNPFLFAN PGSQDRWMN GSNVMRKLA SSCGAKNPN LLTSTRFRK HIATTLQLM SMEDNEMEQ VATFMGHTK KTHAEFYRL PQDIYQTAK VAKILLLLE KGKGEQFKG KSLNEIELE RDIYYSSES ESETDEAIP LAEKVLRKA ANAPLQNKE EKKNENAEN NTNTVTKQL NLEEEVCSS NMEQEADAE VEDVNGNSK KTENTNKSI GKKKEKENN SEMEVDTNN NRKNAGGKN KPLGKKSES SNSVRHRWS EKEKRIVLH HFRGHILKK VAPKKHECE ELISQHNDI LGCKSDDNE QGLDEGGNK SPTINIQSV VTFRDGIRV DKTEGKRND TKEEESVLP NDENQELFL ADSELTIYS ANEYSNSKV QLSKRKQRH MSEVSIQQL EKMPKGSIE RKQFIAMLR KKGNFLSPA QNEIKVVKR PVKEVDPSN ILPCVYCKG FYMKRYLKR HVKTCLQRP TTSGAEDFR TADAQSYLA FRSVDQKFI DELRFKEGI LNMRCDNIA FIAKSDPLI LSFAAFYAK RHRHNHLNK VTKNKIREL ARLWKDMKK YINDKNFFA ALKPENFKY FVLSTQNIT GYNHNSQKF EKAPSLALH MGTTLRKVC EFAFSEIQQ KNHIFISGL DILKKTKDI KVLKELIKN NWSAEVSSV ANQNLVENQ WNKPTIIPL TSDIKKLNN YVKLRAEQC ADKLKKQED DKKSFNDLQ KCCFTLLIT LNRRRIGEL ERLELESYL KKNNDLISE EFEQKLTET EKFLLKKYK RIRIRGKRH KPVPVLISK EVQEYINLL LLVRKNFVP ENGYAALRW LAQSSGAEY PKTLTSGKL RKHIATISQ ISDLSSSEL EQLCTFLGH TMTTHQDFY RLPQEIYQT AKLTKLLLK SQNESVDVD DMNQELGSD GDNDQSDDD EVINVVPST SEGIGQNLK EKIITNSIN VDKRSEQKS NGKGKSKKA SRYNLDENQ LKISVMIRK PPTESEIEE FSKKYPIME NKDWKKIKV FVYNEYSKK
- >TC001429 RA
atggaacag gttgctaca tttatgggt catactaaa aaaacacat gcagaattc tatcgatta ccgcaagat atttaccag acggccaaa gtggctaag attcttttg ttattagaa aaaggaaaa ggcgaacag tttaaagga aaaagtcta aatgaaatt gaattagag cgagatatc tactacagt tcggaatca gaaagtgaa acagatgaa gcaatacca cttgcagaa aaagttctt agaaaagcg gcaaatgct ccattacaa aataaagaa gaaaagaaa aacgaaaat gcagaaaat aatacaaac acagtcaca aaacaatta aatctagaa gaggaagta tgtagtagc aacatggaa caggaagca gatgcagaa gtagaagat gttaatggg aattcaaag aaaacagaa aatacgaat aaaagtatt ggaaagaaa aaagaaaaa gaaaataat tcagaaatg gaagtagac actaataac aatagaaag aatgcagga ggaaagaat aagcctctt ggaaagaaa agtgaatct tcaaactct gtgcgacat cgatggtca gaaaaagaa aaaaggatc gtgctgcat cattttcgt ggtcacatt ctaaagaaa gttgctccg aagaaacat gaatgcgag gaacttatc tctcaacac aatgatatt cttgggtgt aaaagcgat gataatgag cagggttta gatgagggg ggtaacaag tcgccaaca ataaatata cagagtgtg gttaccttt cgtgacggg atcagagtt gataaaaca gaaggtaaa agaaacgat acaaaagaa gaagaaagt gttttgcct aatgatgaa aatcaagag ctgtttttg gctgattcg gaacttaca atttacagt gcaaatgag tattcaaat tcaaaagta cagctctcc aaaaggaaa caaagacat atgtctgaa gtgtcaatt cagcagctc gaaaaaatg cccaaagga agtattgag cgaaaacaa ttcatagct atgctcaga aaaaaagga aatttttta tcacctgca caaaacgaa atcaaagtt gttaaaaga cctgttaag gaagtggat ccatcaaac attcttcct tgcgtatac tgcaaaggt ttttacatg aaaagatat ctaaaacgt catgtgaaa acatgtcta caacgacct accaccagt ggtgctgaa gactttagg acagcggat gctcaaagt tacttagca tttcgaagt gttgatcaa aagtttatt gatgaactt agattcaaa gaaggcatt ttaaatatg agatgcgat aatatagct tttatcgcc aaatctgat ccactaatt ttgtccttt gctgcattt tatgcaaag cgccatagg cataatcat ttgaataaa gtgacaaaa aataaaatc cgagaactg gcgcgcttg tggaaagac atgaaaaaa tatattaac gataaaaac ttttttgca gctcttaaa cctgaaaat tttaaatac ttcgtttta agcacgcaa aatataacc ggttacaat cacaactct caaaagttt gaaaaagca ccatcacta gccctccac atgggaact acgttaaga aaagtttgt gaatttgcg ttctcagaa attcaacaa aaaaatcat atttttata tcaggtttg gacatttta aaaaagaca aaagacata aaggtgttg aaagagttg atcaaaaat aattggagc gcagaagta agcagtgtt gcaaatcaa aatctagtt gaaaatcag tggaacaag ccaactatt attcctcta acttcagat attaaaaaa ctcaataac tatgttaaa cttagggct gaacaatgt gccgacaaa ttaaaaaaa caagaagat gacaaaaaa tctttcaat gatttacaa aaatgctgc tttactttg ctaattact ttgaatcgt agacgaatt ggcgaactt gagcgattg gaattagaa agttactta aagaaaaat aatgattta atatcagaa gaatttgaa cagaaattg acggagaca gaaaaattt cttttgaaa aagtacaag cgtattcgc attagaggc aaacgtcac aaacccgta cctgtttta atttccaag gaagtacag gaatacata aatttatta ttgttagta cgaaaaaat ttcgtacct gaaaacggt tacgctgca ttaagatgg ttagctcaa tcatcaggt gcagagtat cctaagaca ttaacgtca ggcaaatta agaaaacac atcgcgaca atttcacaa atttctgac ttatcctct tccgaactg gaacagcta tgtacattt ctaggacac actatgacg actcatcaa gatttttat aggttgcct caagaaatt tatcaaact gcaaaatta acaaaactt ttgctaaaa tctcaaaac gaatcagtt gacgtagac gatatgaat caggaactt ggctcagac ggtgataac gatcaaagc gatgatgac gaagtgatc aatgttgta ccaagtaca agtaatggt tctggtgaa ggtatcggc caaaacttg aaagaaaaa ataataaca aattcaata aatgttgat aaacggagt gaacagaag tcaaacgga aaaggcaaa tctaaaaaa gcgagccgt tataatctt gatgaaaat caactgaaa atctcagta atgattcga aaaccacca actgagtca gaaattgag gaatttagt aagaaatat ccaatcatg gaaaataag gattggaag aaaattaaa gtatttgtt tacaatgaa tattccaaa aaataa
- >TC001429 RB
atggaacag gttgctaca tttatgggt catactaaa aaaacacat gcagaattc tatcgatta ccgcaagat atttaccag acggccaaa gtggctaag attcttttg ttattagaa aaaggaaaa ggcgaacag tttaaagga aaaagtcta aatgaaatt gaattagag cgagatatc tactacagt tcggaatca gaaagtgaa acagatgaa gcaatacca cttgcagaa aaagttctt agaaaagcg gcaaatgct ccattacaa aataaagaa gaaaagaaa aacgaaaat gcagaaaat aatacaaac acagtcaca aaacaatta aatctagaa gaggaagta tgtagtagc aacatggaa caggaagca gatgcagaa gtagaagat gttaatggg aattcaaag aaaacagaa aatacgaat aaaagtatt ggaaagaaa aaagaaaaa gaaaataat tcagaaatg gaagtagac actaataac aatagaaag aatgcagga ggaaagaat aagcctctt ggaaagaaa agtgaatct tcaaactct gtgcgacat cgatggtca gaaaaagaa aaaaggatc gtgctgcat cattttcgt ggtcacatt ctaaagaaa gttgctccg aagaaacat gaatgcgag gaacttatc tctcaacac aatgatatt cttgggtgt aaaagcgat gataatgag cagggttta gatgagggg ggtaacaag tcgccaaca ataaatata cagagtgtg gttaccttt cgtgacggg atcagagtt gataaaaca gaaggtaaa agaaacgat acaaaagaa gaagaaagt gttttgcct aatgatgaa aatcaagag ctgtttttg gctgattcg gaacttaca atttacagt gcaaatgag tattcaaat tcaaaagta cagctctcc aaaaggaaa caaagacat atgtctgaa gtgtcaatt cagcagctc gaaaaaatg cccaaagga agtattgag cgaaaacaa ttcatagct atgctcaga aaaaaagga aatttttta tcacctgca caaaacgaa atcaaagtt gttaaaaga cctgttaag gaagtggat ccatcaaac attcttcct tgcgtatac tgcaaaggt ttttacatg aaaagatat ctaaaacgt catgtgaaa acatgtcta caacgacct accaccagt ggtgctgaa gactttagg acagcggat gctcaaagt tacttagca tttcgaagt gttgatcaa aagtttatt gatgaactt agattcaaa gaaggcatt ttaaatatg agatgcgat aatatagct tttatcgcc aaatctgat ccactaatt ttgtccttt gctgcattt tatgcaaag cgccatagg cataatcat ttgaataaa gtgacaaaa aataaaatc cgagaactg gcgcgcttg tggaaagac atgaaaaaa tatattaac gataaaaac ttttttgca gctcttaaa cctgaaaat tttaaatac ttcgtttta agcacgcaa aatataacc ggttacaat cacaactct caaaagttt gaaaaagca ccatcacta gccctccac atgggaact acgttaaga aaagtttgt gaatttgcg ttctcagaa attcaacaa aaaaatcat atttttata tcaggtttg gacatttta aaaaagaca aaagacata aaggtgttg aaagagttg atcaaaaat aattggagc gcagaagta agcagtgtt gcaaatcaa aatctagtt gaaaatcag tggaacaag ccaactatt attcctcta acttcagat attaaaaaa ctcaataac tatgttaaa cttagggct gaacaatgt gccgacaaa ttaaaaaaa caagaagat gacaaaaaa tctttcaat gatttacaa aaatgctgc tttactttg ctaattact ttgaatcgt agacgaatt ggcgaactt gagcgattg gaattagaa agttactta aagaaaaat aatgattta atatcagaa gaatttgaa cagaaattg acggagaca gaaaaattt cttttgaaa aagtacaag cgtattcgc attagaggc aaacgtcac aaacccgta cctgtttta atttccaag gaagtacag gaatacata aatttatta ttgttagta cgaaaaaat ttcgtacct gaaaacggt tacgctgca ttaagatgg ttagctcaa tcatcaggt gcagagtat cctaagaca ttaacgtca ggcaaatta agaaaacac atcgcgaca atttcacaa atttctgac ttatcctct tccgaactg gaacagcta tgtacattt ctaggacac actatgacg actcatcaa gatttttat aggttgcct caagaaatt tatcaaact gcaaaatta acaaaactt ttgctaaaa tctcaaaac gaatcagtt gacgtagac gatatgaat caggaactt ggctcagac ggtgataac gatcaaagc gatgatgac gaagtgatc aatgttgta ccaagtaca agtgaaggt atcggccaa aacttgaaa gaaaaaata ataacaaat tcaataaat gttgataaa cggagtgaa cagaagtca aacggaaaa ggcaaatct aaaaaagcg agccgttat aatcttgat gaaaatcaa ctgaaaatc tcagtaatg attcgaaaa ccaccaact gagtcagaa attgaggaa tttagtaag aaatatcca atcatggaa aataaggat tggaagaaa attaaagta tttgtttac aatgaatat tccaaaaaa taa
- >TC001429 RC
atgttcaag gcaccctcg cttgcgttg catatgggc actaatttg aaaatctta tgtgacgta gccttaaaa gttgtcatt gagaagaga catttgccg aacttaaga tgggaagat gatagagaa aagaaaaca gaaatcaag gaactgaaa aaactaata agtggtcat tggtgtaat gagctttca agtttggct ttgaaaaac ttaaaagaa aaacattgg gaaaaacca acagaacta cctctaaca agcgacatt ttaacgtta caaacgttt attagtgaa caggcggac aaagcatat aataacttg ataaatgag ggaaaaaca aatatgcga caaaattat aaaacttta gcacaatgt gttttggct ttaacaata atgttcaat cgaaaacgt gttggcgat gttcagtat ttaaaattg gcaacatac atttcggct gacaagaac tcggttatt gaaggtgaa caagcattt atgcgttct ctcacatcg gtggaacaa attttaagt aaacgattt aaaagagtg gtcactggt ggcaaaggc tctaaacca gttccaatt ttattttcc ataaaacta caaaaaatt atagattgt ctaattaaa atcagaacc gaaacatct attgtgcca acatcaaat cctttccta tttgcaaat cctggatct caagacaga tggatgaat ggaagcaac gtaatgaga aaacttgct tctagttgt ggagcaaaa aatcctaat cttttaaca tctaccaga tttagaaaa catattgct acgacttta caactaatg agtatggaa gacaatgaa atggaacag gttgctaca tttatgggt catactaaa aaaacacat gcagaattc tatcgatta ccgcaagat atttaccag acggccaaa gtggctaag attcttttg ttattagaa aaaggaaaa ggcgaacag tttaaagga aaaagtcta aatgaaatt gaattagag cgagatatc tactacagt tcggaatca gaaagtgaa acagatgaa gcaatacca cttgcagaa aaagttctt agaaaagcg gcaaatgct ccattacaa aataaagaa gaaaagaaa aacgaaaat gcagaaaat aatacaaac acagtcaca aaacaatta aatctagaa gaggaagta tgtagtagc aacatggaa caggaagca gatgcagaa gtagaagat gttaatggg aattcaaag aaaacagaa aatacgaat aaaagtatt ggaaagaaa aaagaaaaa gaaaataat tcagaaatg gaagtagac actaataac aatagaaag aatgcagga ggaaagaat aagcctctt ggaaagaaa agtgaatct tcaaactct gtgcgacat cgatggtca gaaaaagaa aaaaggatc gtgctgcat cattttcgt ggtcacatt ctaaagaaa gttgctccg aagaaacat gaatgcgag gaacttatc tctcaacac aatgatatt cttgggtgt aaaagcgat gataatgag cagggttta gatgagggg ggtaacaag tcgccaaca ataaatata cagagtgtg gttaccttt cgtgacggg atcagagtt gataaaaca gaaggtaaa agaaacgat acaaaagaa gaagaaagt gttttgcct aatgatgaa aatcaagag ctgtttttg gctgattcg gaacttaca atttacagt gcaaatgag tattcaaat tcaaaagta cagctctcc aaaaggaaa caaagacat atgtctgaa gtgtcaatt cagcagctc gaaaaaatg cccaaagga agtattgag cgaaaacaa ttcatagct atgctcaga aaaaaagga aatttttta tcacctgca caaaacgaa atcaaagtt gttaaaaga cctgttaag gaagtggat ccatcaaac attcttcct tgcgtatac tgcaaaggt ttttacatg aaaagatat ctaaaacgt catgtgaaa acatgtcta caacgacct accaccagt ggtgctgaa gactttagg acagcggat gctcaaagt tacttagca tttcgaagt gttgatcaa aagtttatt gatgaactt agattcaaa gaaggcatt ttaaatatg agatgcgat aatatagct tttatcgcc aaatctgat ccactaatt ttgtccttt gctgcattt tatgcaaag cgccatagg cataatcat ttgaataaa gtgacaaaa aataaaatc cgagaactg gcgcgcttg tggaaagac atgaaaaaa tatattaac gataaaaac ttttttgca gctcttaaa cctgaaaat tttaaatac ttcgtttta agcacgcaa aatataacc ggttacaat cacaactct caaaagttt gaaaaagca ccatcacta gccctccac atgggaact acgttaaga aaagtttgt gaatttgcg ttctcagaa attcaacaa aaaaatcat atttttata tcaggtttg gacatttta aaaaagaca aaagacata aaggtgttg aaagagttg atcaaaaat aattggagc gcagaagta agcagtgtt gcaaatcaa aatctagtt gaaaatcag tggaacaag ccaactatt attcctcta acttcagat attaaaaaa ctcaataac tatgttaaa cttagggct gaacaatgt gccgacaaa ttaaaaaaa caagaagat gacaaaaaa tctttcaat gatttacaa aaatgctgc tttactttg ctaattact ttgaatcgt agacgaatt ggcgaactt gagcgattg gaattagaa agttactta aagaaaaat aatgattta atatcagaa gaatttgaa cagaaattg acggagaca gaaaaattt cttttgaaa aagtacaag cgtattcgc attagaggc aaacgtcac aaacccgta cctgtttta atttccaag gaagtacag gaatacata aatttatta ttgttagta cgaaaaaat ttcgtacct gaaaacggt tacgctgca ttaagatgg ttagctcaa tcatcaggt gcagagtat cctaagaca ttaacgtca ggcaaatta agaaaacac atcgcgaca atttcacaa atttctgac ttatcctct tccgaactg gaacagcta tgtacattt ctaggacac actatgacg actcatcaa gatttttat aggttgcct caagaaatt tatcaaact gcaaaatta acaaaactt ttgctaaaa tctcaaaac gaatcagtt gacgtagac gatatgaat caggaactt ggctcagac ggtgataac gatcaaagc gatgatgac gaagtgatc aatgttgta ccaagtaca agtgaaggt atcggccaa aacttgaaa gaaaaaata ataacaaat tcaataaat gttgataaa cggagtgaa cagaagtca aacggaaaa ggcaaatct aaaaaagcg agccgttat aatcttgat gaaaatcaa ctgaaaatc tcagtaatg attcgaaaa ccaccaact gagtcagaa attgaggaa tttagtaag aaatatcca atcatggaa aataaggat tggaagaaa attaaagta tttgtttac aatgaatat tccaaaaaa taa
- >TC001429 RA
caaaattgt tgcagcaca tgtgacaga cgtgggtga agttactca tttacctaa acgtcaaag gtctacata ctccagacc accttcttc agaatcaag aagtgtaag tttttttgg aacaattat aggcagttt tttcaaact tataatctt gtttaattt aaaaattac tcaaaaatt agctttttc tattaaata cgtgatgct taaattatt ttttatttt ttctgtaat atttttaaa taattatta ttaattaaa cattaccta aaaattgaa aagtttttc taaaaaaaa tagatttaa tcgttgcca gtaaattga aattttgtt gaaggaaaa attaaatgt tttttttta agagcacaa gcagttttc aagattttg cgagctctg gtagtgaat atgtacctc ctgataaac atccaacta ctaatttta attggtcag attcagatt ctgcccagc cttctcctg atattccat atctaaagc agattacca catctcaag atcaaatta aaatagatc ctacttcgt tacctggtg taggttgaa ttaattatt ttaaatagt gtttaattt ccattatat tcagtgttc aaaagatac gtgcagtcc acttgaaca aaatccttg taacgaaac cgagccaca tgctcttaa tgattctct tacccgtat tcaaaagcc aaaccaaga ttatattaa gaagcaatc tgctaggga aaggccaac aatatgtcc tatttgttt taatgatgt tagcaccca ttttacacg ccatctact tcgacacca caaaaagag taaagaagt gcagctcct tgaaagtct taaacctcg gagcaaaga acgtttagc tctaattga gtcattaag aaagcaagg atactttca tctaaagaa tgaaaaagg gattttgaa tcctgtcag aacctcaaa aaacccaga tgttgagta ttttgtttg cacatattg cttaggtta ctttagtaa gaaactttt gttataagc atgtcaaag gttgtaaaa aacgccctc cagacgaag cgacgcctg gaaataatt gtctagcca gatctcaaa cttttgcat cattacttg tcgtaaaaa atgatgaat ttttgaaaa aatctcgtc tgaaaaagg aagtttttg gaataatgc gtccagacg agattagtg cagtagcga aaaatgatt caataattt gtttgtatg gagaaacgc tattaaata aacacaaaa gacaacaaa ttgctaccg ttgtttcaa ataagataa gagaaatgg ccagattat tgattgctt taaaattaa tggacagaa caatttgta ctctttttg acgctttaa agcctgaaa attttaggc atttggtat cggcagcta aagaaataa gtggctaca atgccgaaa caaaaatgt tcaaggcac cctcgcttg cgttgcata tgggcacta atttgaaaa tcttatgtg acgtagcct taaaagttg tcattgaga agagacatt tgccgaact taagatggg aagatgata gagaaaaga aaacagaaa tcaaggaac tgaaaaaac taataagtg gtcattggt gtaatgagc tttcaagtt tggctttga aaaacttaa aagaaaaac attgggaaa aaccaacag aactacctc taacaagcg acattttaa cgttacaaa cgtttatta gtgaacagg cggacaaag catataata acttgataa atgagggaa aaacaaata tgcgacaaa attataaaa ctttagcac aatgtgttt tggctttaa caataatgt tcaatcgaa aacgtgttg gcgatgttc agtatttaa aattggcaa catacattt cggctgaca agaactcgg ttattgaag gtgaacaag catttatgc gttctctca catcggtgg aacaaattt taagtaaac gatttaaaa gagtggtca ctggtggca aaggctcta aaccagttc caattttat tttccataa aactacaaa aaattatag attgtctaa ttaaaatca gaaccgaaa catctattg tgccaacat caaatcctt tcctatttg caaatcctg gatctcaag acagatgga tgaatggaa gcaacgtaa tgagaaaac ttgcttcta gttgtggag caaaaaatc ctaatcttt taacatcta ccagattta gaaaacata ttgctacga ctttacaac taatgagta tggaagaca atgaaatgg aacaggttg ctacattta tgggtcata ctaaaaaaa cacatgcag aattctatc gattaccgc aagatattt accagacgg ccaaagtgg ctaagattc ttttgttat tagaaaaag gaaaaggcg aacagttta aaggaaaaa gtctaaatg aaattgaat tagagcgag atatctact acagttcgg aatcagaaa gtgaaacag atgaagcaa taccacttg cagaaaaag ttcttagaa aagcggcaa atgctccat tacaaaata aagaagaaa agaaaaacg aaaatgcag aaaataata caaacacag tcacaaaac aattaaatc tagaagagg aagtatgta gtagcaaca tggaacagg aagcagatg cagaagtag aagatgtta atgggaatt caaagaaaa cagaaaata cgaataaaa gtattggaa agaaaaaag aaaaagaaa ataattcag aaatggaag tagacacta ataacaata gaaagaatg caggaggaa agaataagc ctcttggaa agaaaagtg aatcttcaa actctgtgc gacatcgat ggtcagaaa aagaaaaaa ggatcgtgc tgcatcatt ttcgtggtc acattctaa agaaagttg ctccgaaga aacatgaat gcgaggaac ttatctctc aacacaatg atattcttg ggtgtaaaa gcgatgata atgagcagg gtttagatg aggggggta acaagtcgc caacaataa atatacaga gtgtggtta cctttcgtg acgggatca gagttgata aaacagaag gtaaaagaa acgatacaa aagaagaag aaagtgttt tgcctaatg atgaaaatc aagagctgt ttttggctg attcggaac ttacaattt acagtgcaa atgagtatt caaattcaa aagtacagc tctccaaaa ggaaacaaa gacatatgt ctgaagtgt caattcagc agctcgaaa aaatgccca aaggaagta ttgagcgaa aacaattca tagctatgc tcagaaaaa aaggaaatt ttttatcac ctgcacaaa acgaaatca aagttgtta aaagacctg ttaaggaag tggatccat caaacattc ttccttgcg tatactgca aaggttttt acatgaaaa gatatctaa aacgtcatg tgaaaacat gtctacaac gacctacca ccagtggtg ctgaagact ttaggacag cggatgctc aaagttact tagcatttc gaagtgttg atcaaaagt ttattgatg aacttagat tcaaagaag gcattttaa atatgagat gcgataata tagctttta tcgccaaat ctgatccac taattttgt cctttgctg cattttatg caaagcgcc ataggcata atcatttga ataaagtga caaaaaata aaatccgag aactggcgc gcttgtgga aagacatga aaaaatata ttaacgata aaaactttt ttgcagctc ttaaacctg aaaatttta aatacttcg ttttaagca cgcaaaata taaccggtt acaatcaca actctcaaa agtttgaaa aagcaccat cactagccc tccacatgg gaactacgt taagaaaag tttgtgaat ttgcgttct cagaaattc aacaaaaaa atcatattt ttatatcag gtttggaca ttttaaaaa agacaaaag acataaagg tgttgaaag agttgatca aaaataatt ggagcgcag aagtaagca gtgttgcaa atcaaaatc tagttgaaa atcagtgga acaagccaa ctattattc ctctaactt cagatatta aaaaactca ataactatg ttaaactta gggctgaac aatgtgccg acaaattaa aaaaacaag aagatgaca aaaaatctt tcaatgatt tacaaaaat gctgcttta ctttgctaa ttactttga atcgtagac gaattggcg aacttgagc gattggaat tagaaagtt acttaaaga aaaataatg atttaatat cagaagaat ttgaacaga aattgacgg agacagaaa aatttcttt tgaaaaagt acaagcgta ttcgcatta gaggcaaac gtcacaaac ccgtacctg ttttaattt ccaaggaag tacaggaat acataaatt tattattgt tagtacgaa aaaatttcg tacctgaaa acggttacg ctgcattaa gatggttag ctcaatcat caggtgcag agtatccta agacattaa cgtcaggca aattaagaa aacacatcg cgacaattt cacaaattt ctgacttat cctcttccg aactggaac agctatgta catttctag gacacacta tgacgactc atcaagatt tttataggt tgcctcaag aaatttatc aaactgcaa aattaacaa aacttttgc taaaatctc aaaacgaat cagttgacg tagacgata tgaatcagg aacttggct cagacggtg ataacgatc aaagcgatg atgacgaag tgatcaatg ttgtaccaa gtacaagta atggttctg gtgaaggta tcggccaaa acttgaaag aaaaaataa taacaaatt caataaatg ttgataaac ggagtgaac agaagtcaa acggaaaag gcaaatcta aaaaagcga gccgttata atcttgatg aaaatcaac tgaaaatct cagtaatga ttcgaaaac caccaactg agtcagaaa ttgaggaat ttagtaaga aatatccaa tcatggaaa ataaggatt ggaagaaaa ttaaagtat ttgtttaca atgaatatt ccaaaaaat aataactgt tttgctgta atatgaata attttttgt tctgttt
- >TC001429 RB
caaaattgt tgcagcaca tgtgacaga cgtgggtga agttactca tttacctaa acgtcaaag gtctacata ctccagacc accttcttc agaatcaag aagtgtaag tttttttgg aacaattat aggcagttt tttcaaact tataatctt gtttaattt aaaaattac tcaaaaatt agctttttc tattaaata cgtgatgct taaattatt ttttatttt ttctgtaat atttttaaa taattatta ttaattaaa cattaccta aaaattgaa aagtttttc taaaaaaaa tagatttaa tcgttgcca gtaaattga aattttgtt gaaggaaaa attaaatgt tttttttta agagcacaa gcagttttc aagattttg cgagctctg gtagtgaat atgtacctc ctgataaac atccaacta ctaatttta attggtcag attcagatt ctgcccagc cttctcctg atattccat atctaaagc agattacca catctcaag atcaaatta aaatagatc ctacttcgt tacctggtg taggttgaa ttaattatt ttaaatagt gtttaattt ccattatat tcagtgttc aaaagatac gtgcagtcc acttgaaca aaatccttg taacgaaac cgagccaca tgctcttaa tgattctct tacccgtat tcaaaagcc aaaccaaga ttatattaa gaagcaatc tgctaggga aaggccaac aatatgtcc tatttgttt taatgatgt tagcaccca ttttacacg ccatctact tcgacacca caaaaagag taaagaagt gcagctcct tgaaagtct taaacctcg gagcaaaga acgtttagc tctaattga gtcattaag aaagcaagg atactttca tctaaagaa tgaaaaagg gattttgaa tcctgtcag aacctcaaa aaacccaga tgttgagta ttttgtttg cacatattg cttaggtta ctttagtaa gaaactttt gttataagc atgtcaaag gttgtaaaa aacgccctc cagacgaag cgacgcctg gaaataatt gtctagcca gatctcaaa cttttgcat cattacttg tcgtaaaaa atgatgaat ttttgaaaa aatctcgtc tgaaaaagg aagtttttg gaataatgc gtccagacg agattagtg cagtagcga aaaatgatt caataattt gtttgtatg gagaaacgc tattaaata aacacaaaa gacaacaaa ttgctaccg ttgtttcaa ataagataa gagaaatgg ccagattat tgattgctt taaaattaa tggacagaa caatttgta ctctttttg acgctttaa agcctgaaa attttaggc atttggtat cggcagcta aagaaataa gtggctaca atgccgaaa caaaaatgt tcaaggcac cctcgcttg cgttgcata tgggcacta atttgaaaa tcttatgtg acgtagcct taaaagttg tcattgaga agagacatt tgccgaact taagatggg aagatgata gagaaaaga aaacagaaa tcaaggaac tgaaaaaac taataagtg gtcattggt gtaatgagc tttcaagtt tggctttga aaaacttaa aagaaaaac attgggaaa aaccaacag aactacctc taacaagcg acattttaa cgttacaaa cgtttatta gtgaacagg cggacaaag catataata acttgataa atgagggaa aaacaaata tgcgacaaa attataaaa ctttagcac aatgtgttt tggctttaa caataatgt tcaatcgaa aacgtgttg gcgatgttc agtatttaa aattggcaa catacattt cggctgaca agaactcgg ttattgaag gtgaacaag catttatgc gttctctca catcggtgg aacaaattt taagtaaac gatttaaaa gagtggtca ctggtggca aaggctcta aaccagttc caattttat tttccataa aactacaaa aaattatag attgtctaa ttaaaatca gaaccgaaa catctattg tgccaacat caaatcctt tcctatttg caaatcctg gatctcaag acagatgga tgaatggaa gcaacgtaa tgagaaaac ttgcttcta gttgtggag caaaaaatc ctaatcttt taacatcta ccagattta gaaaacata ttgctacga ctttacaac taatgagta tggaagaca atgaaatgg aacaggttg ctacattta tgggtcata ctaaaaaaa cacatgcag aattctatc gattaccgc aagatattt accagacgg ccaaagtgg ctaagattc ttttgttat tagaaaaag gaaaaggcg aacagttta aaggaaaaa gtctaaatg aaattgaat tagagcgag atatctact acagttcgg aatcagaaa gtgaaacag atgaagcaa taccacttg cagaaaaag ttcttagaa aagcggcaa atgctccat tacaaaata aagaagaaa agaaaaacg aaaatgcag aaaataata caaacacag tcacaaaac aattaaatc tagaagagg aagtatgta gtagcaaca tggaacagg aagcagatg cagaagtag aagatgtta atgggaatt caaagaaaa cagaaaata cgaataaaa gtattggaa agaaaaaag aaaaagaaa ataattcag aaatggaag tagacacta ataacaata gaaagaatg caggaggaa agaataagc ctcttggaa agaaaagtg aatcttcaa actctgtgc gacatcgat ggtcagaaa aagaaaaaa ggatcgtgc tgcatcatt ttcgtggtc acattctaa agaaagttg ctccgaaga aacatgaat gcgaggaac ttatctctc aacacaatg atattcttg ggtgtaaaa gcgatgata atgagcagg gtttagatg aggggggta acaagtcgc caacaataa atatacaga gtgtggtta cctttcgtg acgggatca gagttgata aaacagaag gtaaaagaa acgatacaa aagaagaag aaagtgttt tgcctaatg atgaaaatc aagagctgt ttttggctg attcggaac ttacaattt acagtgcaa atgagtatt caaattcaa aagtacagc tctccaaaa ggaaacaaa gacatatgt ctgaagtgt caattcagc agctcgaaa aaatgccca aaggaagta ttgagcgaa aacaattca tagctatgc tcagaaaaa aaggaaatt ttttatcac ctgcacaaa acgaaatca aagttgtta aaagacctg ttaaggaag tggatccat caaacattc ttccttgcg tatactgca aaggttttt acatgaaaa gatatctaa aacgtcatg tgaaaacat gtctacaac gacctacca ccagtggtg ctgaagact ttaggacag cggatgctc aaagttact tagcatttc gaagtgttg atcaaaagt ttattgatg aacttagat tcaaagaag gcattttaa atatgagat gcgataata tagctttta tcgccaaat ctgatccac taattttgt cctttgctg cattttatg caaagcgcc ataggcata atcatttga ataaagtga caaaaaata aaatccgag aactggcgc gcttgtgga aagacatga aaaaatata ttaacgata aaaactttt ttgcagctc ttaaacctg aaaatttta aatacttcg ttttaagca cgcaaaata taaccggtt acaatcaca actctcaaa agtttgaaa aagcaccat cactagccc tccacatgg gaactacgt taagaaaag tttgtgaat ttgcgttct cagaaattc aacaaaaaa atcatattt ttatatcag gtttggaca ttttaaaaa agacaaaag acataaagg tgttgaaag agttgatca aaaataatt ggagcgcag aagtaagca gtgttgcaa atcaaaatc tagttgaaa atcagtgga acaagccaa ctattattc ctctaactt cagatatta aaaaactca ataactatg ttaaactta gggctgaac aatgtgccg acaaattaa aaaaacaag aagatgaca aaaaatctt tcaatgatt tacaaaaat gctgcttta ctttgctaa ttactttga atcgtagac gaattggcg aacttgagc gattggaat tagaaagtt acttaaaga aaaataatg atttaatat cagaagaat ttgaacaga aattgacgg agacagaaa aatttcttt tgaaaaagt acaagcgta ttcgcatta gaggcaaac gtcacaaac ccgtacctg ttttaattt ccaaggaag tacaggaat acataaatt tattattgt tagtacgaa aaaatttcg tacctgaaa acggttacg ctgcattaa gatggttag ctcaatcat caggtgcag agtatccta agacattaa cgtcaggca aattaagaa aacacatcg cgacaattt cacaaattt ctgacttat cctcttccg aactggaac agctatgta catttctag gacacacta tgacgactc atcaagatt tttataggt tgcctcaag aaatttatc aaactgcaa aattaacaa aacttttgc taaaatctc aaaacgaat cagttgacg tagacgata tgaatcagg aacttggct cagacggtg ataacgatc aaagcgatg atgacgaag tgatcaatg ttgtaccaa gtacaagtg aaggtatcg gccaaaact tgaaagaaa aaataataa caaattcaa taaatgttg ataaacgga gtgaacaga agtcaaacg gaaaaggca aatctaaaa aagcgagcc gttataatc ttgatgaaa atcaactga aaatctcag taatgattc gaaaaccac caactgagt cagaaattg aggaattta gtaagaaat atccaatca tggaaaata aggattgga agaaaatta aagtatttg tttacaatg aatattcca aaaaataat aactgtttt gctgtaata tgaataatt ttttgttct gttt
- >TC001429 RC
caaaattgt tgcagcaca tgtgacaga cgtgggtga agttactca tttacctaa acgtcaaag gtctacata ctccagacc accttcttc agaatcaag aagtagcac aagcagttt tcaagattt tgcgagctc tggtagtga atatgtacc tcctgataa acatccaac tactaattt taattggtc agattcaga ttctgccca gccttctcc tgatattcc atatctaaa gcaggtaaa taattatta gattgtggt ttatttcgt cattatgac tttgaaact tacttttag attaccaca tctcaagat caaattaaa atagatcct acttcgtta cctggtgta ggttgaatt aattatttt aaatagtgt ttaatttcc attatattc agtgttcaa aagatacgt gcagtccac ttgaacaaa atccttgta acgaaaccg agccacatg ctcttaatg attctctta cccgtattc aaaagccaa accaagatt atattaaga agcaatctg ctagggaaa ggccaacaa tatgtccta tttgtttta atgatgtta gcacccatt ttacacgcc atctacttc gacaccaca aaaagagta aagaagtgc agctccttg aaagtctta aacctcgga gcaaagaac gtttagctc taattgagt cattaagaa agcaaggat actttcatc taaagaatg aaaaaggga ttttgaatc ctgtcagaa cctcaaaaa acccagatg ttgagtatt ttgtttgca catattgct taggttact ttagtaaga aacttttgt tataagcat gtcaaaggt tgtaaaaaa cgccctcca gacgaagcg acgcctgga aataattgt ctagccaga tctcaaact tttgcatca ttacttgtc gtaaaaaat gatgaattt ttgaaaaaa tctcgtctg aaaaaggaa gtttttgga ataatgcgt ccagacgag attagtgca gtagcgaaa aatgattca ataatttgt ttgtatgga gaaacgcta ttaaataaa cacaaaaga caacaaatt gctaccgtt gtttcaaat aagataaga gaaatggcc agattattg attgcttta aaattaatg gacagaaca atttgtact ctttttgac gctttaaag cctgaaaat tttaggcat ttggtatcg gcagctaaa gaaataagt ggctacaat gccgaaaca aaaatgttc aaggcaccc tcgcttgcg ttgcatatg ggcactaat ttgaaaatc ttatgtgac gtagcctta aaagttgtc attgagaag agacatttg ccgaactta agatgggaa gatgataga gaaaagaaa acagaaatc aaggaactg aaaaaacta ataagtggt cattggtgt aatgagctt tcaagtttg gctttgaaa aacttaaaa gaaaaacat tgggaaaaa ccaacagaa ctacctcta acaagcgac attttaacg ttacaaacg tttattagt gaacaggcg gacaaagca tataataac ttgataaat gagggaaaa acaaatatg cgacaaaat tataaaact ttagcacaa tgtgttttg gctttaaca ataatgttc aatcgaaaa cgtgttggc gatgttcag tatttaaaa ttggcaaca tacatttcg gctgacaag aactcggtt attgaaggt gaacaagca tttatgcgt tctctcaca tcggtggaa caaatttta agtaaacga tttaaaaga gtggtcact ggtggcaaa ggctctaaa ccagttcca attttattt tccataaaa ctacaaaaa attatagat tgtctaatt aaaatcaga accgaaaca tctattgtg ccaacatca aatcctttc ctatttgca aatcctgga tctcaagac agatggatg aatggaagc aacgtaatg agaaaactt gcttctagt tgtggagca aaaaatcct aatctttta acatctacc agatttaga aaacatatt gctacgact ttacaacta atgagtatg gaagacaat gaaatggaa caggttgct acatttatg ggtcatact aaaaaaaca catgcagaa ttctatcga ttaccgcaa gatatttac cagacggcc aaagtggct aagattctt ttgttatta gaaaaagga aaaggcgaa cagtttaaa ggaaaaagt ctaaatgaa attgaatta gagcgagat atctactac agttcggaa tcagaaagt gaaacagat gaagcaata ccacttgca gaaaaagtt cttagaaaa gcggcaaat gctccatta caaaataaa gaagaaaag aaaaacgaa aatgcagaa aataataca aacacagtc acaaaacaa ttaaatcta gaagaggaa gtatgtagt agcaacatg gaacaggaa gcagatgca gaagtagaa gatgttaat gggaattca aagaaaaca gaaaatacg aataaaagt attggaaag aaaaaagaa aaagaaaat aattcagaa atggaagta gacactaat aacaataga aagaatgca ggaggaaag aataagcct cttggaaag aaaagtgaa tcttcaaac tctgtgcga catcgatgg tcagaaaaa gaaaaaagg atcgtgctg catcatttt cgtggtcac attctaaag aaagttgct ccgaagaaa catgaatgc gaggaactt atctctcaa cacaatgat attcttggg tgtaaaagc gatgataat gagcagggt ttagatgag gggggtaac aagtcgcca acaataaat atacagagt gtggttacc tttcgtgac gggatcaga gttgataaa acagaaggt aaaagaaac gatacaaaa gaagaagaa agtgttttg cctaatgat gaaaatcaa gagctgttt ttggctgat tcggaactt acaatttac agtgcaaat gagtattca aattcaaaa gtacagctc tccaaaagg aaacaaaga catatgtct gaagtgtca attcagcag ctcgaaaaa atgcccaaa ggaagtatt gagcgaaaa caattcata gctatgctc agaaaaaaa ggaaatttt ttatcacct gcacaaaac gaaatcaaa gttgttaaa agacctgtt aaggaagtg gatccatca aacattctt ccttgcgta tactgcaaa ggtttttac atgaaaaga tatctaaaa cgtcatgtg aaaacatgt ctacaacga cctaccacc agtggtgct gaagacttt aggacagcg gatgctcaa agttactta gcatttcga agtgttgat caaaagttt attgatgaa cttagattc aaagaaggc attttaaat atgagatgc gataatata gcttttatc gccaaatct gatccacta attttgtcc tttgctgca ttttatgca aagcgccat aggcataat catttgaat aaagtgaca aaaaataaa atccgagaa ctggcgcgc ttgtggaaa gacatgaaa aaatatatt aacgataaa aactttttt gcagctctt aaacctgaa aattttaaa tacttcgtt ttaagcacg caaaatata accggttac aatcacaac tctcaaaag tttgaaaaa gcaccatca ctagccctc cacatggga actacgtta agaaaagtt tgtgaattt gcgttctca gaaattcaa caaaaaaat catattttt atatcaggt ttggacatt ttaaaaaag acaaaagac ataaaggtg ttgaaagag ttgatcaaa aataattgg agcgcagaa gtaagcagt gttgcaaat caaaatcta gttgaaaat cagtggaac aagccaact attattcct ctaacttca gatattaaa aaactcaat aactatgtt aaacttagg gctgaacaa tgtgccgac aaattaaaa aaacaagaa gatgacaaa aaatctttc aatgattta caaaaatgc tgctttact ttgctaatt actttgaat cgtagacga attggcgaa cttgagcga ttggaatta gaaagttac ttaaagaaa aataatgat ttaatatca gaagaattt gaacagaaa ttgacggag acagaaaaa tttcttttg aaaaagtac aagcgtatt cgcattaga ggcaaacgt cacaaaccc gtacctgtt ttaatttcc aaggaagta caggaatac ataaattta ttattgtta gtacgaaaa aatttcgta cctgaaaac ggttacgct gcattaaga tggttagct caatcatca ggtgcagag tatcctaag acattaacg tcaggcaaa ttaagaaaa cacatcgcg acaatttca caaatttct gacttatcc tcttccgaa ctggaacag ctatgtaca tttctagga cacactatg acgactcat caagatttt tataggttg cctcaagaa atttatcaa actgcaaaa ttaacaaaa cttttgcta aaatctcaa aacgaatca gttgacgta gacgatatg aatcaggaa cttggctca gacggtgat aacgatcaa agcgatgat gacgaagtg atcaatgtt gtaccaagt acaagtgaa ggtatcggc caaaacttg aaagaaaaa ataataaca aattcaata aatgttgat aaacggagt gaacagaag tcaaacgga aaaggcaaa tctaaaaaa gcgagccgt tataatctt gatgaaaat caactgaaa atctcagta atgattcga aaaccacca actgagtca gaaattgag gaatttagt aagaaatat ccaatcatg gaaaataag gattggaag aaaattaaa gtatttgtt tacaatgaa tattccaaa aaataataa ctgttttgc tgtaatatg aataatttt ttgttctgt tt
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