Status
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Gene information
General information
TC034227
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012244,TC012245,TC012243 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 147897..157746
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034227
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03181

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05197

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034227 RA
      MVKIVSSAL KEKDKLLNN NNFQSYSKA MLVAIKDVE 
TLQLWMDKF HKQLKDLHK AIDDNQKKQ HQTLISSLK 
PRKKKHFVS KTVIFQKHN FSTMLNYSE SNDFRSQVV 
EDQWYHKEN VDLPLLNDL VNDSSDLGP ESVLKDCKY 
NPLLNPFDM EWVFGFDIS IPVLSLTAG DRREIFYSA 
TNVGIVYNY CTKTMVHLR GHQSKIVSV SVDRSCRWL 
ATADNGKDN IIMVWDTLN RSVVWTIFN HPAEGHDLI 
LIGLSPSAR LLITITNEH ENTINFWLW TYGRDKPDE 
TYTDDRSLG AAKAIEFHP DLEEHFMIV FEKGVIFFQ 
WDFEQDKLV KNVSPKSPL STRIGYYTD ATYVNCCHE 
CFVSTSKGC ILVFGNTIF AKNYAGQED LDNDKIFVK 
TIKIEKSAL NCLTSVDEM IVTGDSNGT IMFFDKRIK 
LLYWYSRSI EKSIRSMSF VQNPRRKYI FDESQIRDL 
EERSFEGIP DCIQDRSHE YEILMKQKV PKDATLNNR 
PFITRDFFI STVDGQIHM MNYSRQEFI SLFPTGESS 
VAAIDVHDE KPFVIVAYT KGHLIIFNY ETHEEVVSS 
NLPETEPET TITSIKYSP QSLHLACGR SNGELWLLQ 
PATLGQIYV EPFRFSNDK VMKIAFSQN SLQMAYYDS 
NNTIFGFYY DCESEDWIF KGKVRSHYK DVCNILFLD 
EEPFGLYTI GKDRHLIRY TNIAAEYLI YLGQGPTFL 
SEEELDIST RDRIEQKDT PIYFTPYIR KKFSKCGYF 
LIADDKHKY KTVNAKTMM CTAVYLGPA YGCFKDSPV 
CKMEFLPKS NRKYLIFMT KKQIGIQLM SIDGNPFKF 
VGSLGHPDE LLDFQMCKD GKYVFTIGK NDRSVFMWR 
VKTASVETM HILGGVELQ PYYCLIEGG RAGFLYQEM 
RDLFYYMQI LQQGEQICA PRVVSNCIN LGELPDLMR 
ACGYYPSEY EIENLLTDM RYKEYDENG MLKDKVTFC 
EFVKLFINH KPAYGYTIE SLEESFETI TTFSDEYGA 
SDMSREDFI EAITTTGEQ VSNEQIFRC LITLLHETE 
TPDQFGERD FSFMPDTID FDYLTEDIL GIDMDQIER 
LEKDDDNDD DDRGGYYDF FYRS
    CDS sequence
    • >TC034227 RA
      atggtcaaa attgtctca agtgccctt aaagagaag 
gataagttg ttgaataac aacaatttc cagagttat 
tcaaaggcc atgctggtt gccatcaaa gacgtcgaa 
acgttgcag ttgtggatg gataagttt cacaaacaa 
ttgaaagat ttacataaa gcaatagac gacaatcag 
aaaaaacag caccagact ttgatttct tcactcaag 
ccgagaaaa aaaaaacat tttgtttcc aaaactgtc 
atttttcaa aaacacaat ttttcaacg atgttaaac 
tactccgaa agcaacgac tttcggtca caagtcgtt 
gaagaccag tggtatcac aaggagaat gtggatttg 
cccctcttg aacgacctg gtcaacgac tcgagcgat 
ctaggccct gaaagcgtg ctcaaagac tgcaagtac 
aacccctta ctaaaccct ttcgacatg gaatgggtc 
ttcggcttc gacatcagc atcccagtc cttagcttg 
actgcgggc gaccgccgc gaaatattt tactcagcg 
acgaatgtc ggaattgtg tacaattat tgcacaaaa 
acgatggtc catctcaga ggacaccaa agcaaaatc 
gtgagcgtt tcagtggac cgcagttgt cgctggctg 
gccaccgct gacaacgga aaagacaac attatcatg 
gtgtgggac actctaaat cgctcggtc gtttggacg 
attttcaac catccggcc gaaggccac gacttgatc 
ctcatcggg ctaagtcca agcgcccga ttgttaata 
acaatcacc aacgaacac gaaaacacg attaatttt 
tggctgtgg acctacggc agagataaa ccggatgaa 
acgtacaca gacgaccgc agtttaggc gcagccaaa 
gccatcgag ttccatcca gacctggaa gagcacttc 
atgattgtc tttgaaaag ggcgtaatt ttcttccag 
tgggacttc gaacaggac aagttggtc aaaaacgtg 
agccccaaa agccccctt agcaccaga atagggtat 
tataccgat gcaacctac gttaattgt tgccacgaa 
tgtttcgtg agcacctcc aaaggctgc atcctggtt 
tttgggaac acgattttt gccaaaaac tacgccgga 
caggaggat cttgacaac gacaaaatt ttcgtcaag 
actatcaag attgagaaa agcgccttg aactgtctg 
acttcggtc gatgaaatg atagtgacg ggagattca 
aacggaaca atcatgttt ttcgacaaa cggatcaaa 
ctcctctat tggtactcc cgcagtatt gaaaagtcg 
atccgatcg atgagtttc gtgcaaaac ccgcgccga 
aaatacatt tttgacgag tcccaaatc cgcgatttg 
gaagagaga agtttcgag ggaatcccc gattgcatc 
caagaccgc tcgcacgaa tacgaaatt ttgatgaaa 
cagaaagtg ccaaaagac gcgacgctc aacaaccgg 
ccctttatc actcgagac tttttcatc tccaccgtg 
gacggccaa atccacatg atgaattac tccaggcag 
gagtttatt tcgttgttc cccactggc gagagcagc 
gttgcagca attgacgtg catgacgaa aagcctttt 
gtcatagtt gcgtacacc aaaggtcac ctcattata 
ttcaattac gaaacgcac gaagaagtc gtttcgtca 
aacctgcca gagaccgaa cccgaaacc actataacg 
agcatcaaa tattcgccg caaagtttg cacttggct 
tgtgggcgc tcaaatggt gaactgtgg ttgctacaa 
ccggccact ctaggccaa atttacgtg gaacctttc 
agattcagt aacgacaaa gttatgaaa attgcattc 
tcgcaaaat tcgctacaa atggcttat tatgactcg 
aataataca attttcggc ttttactac gactgtgaa 
agcgaagac tggattttt aaaggaaaa gtccggtct 
cactacaaa gacgtttgc aatattttg ttcctggac 
gaagaaccg ttcggcctt tacaccata gggaaggac 
cggcatctg atcagatac acgaacatt gcagccgag 
tatttgatt tatttaggc caagggccg acttttctt 
agtgaagag gagctggac atttccacc cgcgaccga 
atcgagcaa aaggacact ccgatttac ttcactcct 
tatatccgg aaaaagttc agcaaatgt ggctatttt 
ttgatagcc gatgataag cacaaatat aagacagtc 
aatgccaaa acaatgatg tgcaccgct gtgtacttg 
ggaccggcc tatggctgt ttcaaagac agtccggtt 
tgcaaaatg gaatttctc ccaaagtcg aatcggaag 
tatttgatt ttcatgacg aagaaacaa attggaatt 
cagttaatg tcgatcgat ggcaatccg tttaagttt 
gttggaagt ctggggcat cctgacgaa ttgctagac 
ttccaaatg tgtaaagac gggaaatat gtcttcacg 
attggcaaa aacgacaga agtgtgttt atgtggagg 
gtcaaaacg gcgagtgtt gaaacgatg cacattttg 
gggggcgtg gagctacag ccgtactat tgcctcatc 
gagggcgga agggccggt ttcctgtac caggaaatg 
cgcgatttg ttctactac atgcaaatc ctgcagcag 
ggagagcaa atttgtgcg ccccgggtg gtcagcaac 
tgcataaac ctgggggaa ttacccgac ttaatgcgg 
gcttgtggg tactacccc tcggaatat gagattgaa 
aatctccta accgacatg cgttacaaa gaatacgac 
gagaacggc atgctgaag gacaaagtc acgttttgc 
gagtttgtc aaacttttt attaatcat aaacccgct 
tacggttac acaatcgag agtcttgag gagtctttt 
gagacgatc acgacgttt tcggacgaa tatggggcc 
tcagacatg tcgagggag gactttatt gaagctata 
acaacgaca ggggagcaa gtcagcaac gagcagatt 
tttagatgc ttgattact cttttacac gagactgaa 
acgcctgat cagttcggt gagagggac tttagcttt 
atgcccgat accatcgac tttgactac ttgactgaa 
gatattttg ggcatcgac atggaccag attgaacga 
cttgaaaaa gatgacgat aatgacgat gatgaccga 
ggaggctac tacgacttc ttctatcgc tcatga
    mRNA sequence
    • >TC034227 RA
      tgtgacccc cagagacat ggtcaaaat tgtctcaag 
tgcccttaa agagaagga taagttgtt gaataacaa 
caatttcca gagttattc aaaggccat gctggttgc 
catcaaaga cgtcgaaac gttgcagtt gtggatgga 
taagtttca caaacaatt gaaagattt acataaagc 
aatagacga caatcagaa aaaacagca ccagacttt 
gatttcttc actcaagcc gagaaaaaa aaaacattt 
tgtttccaa aactgtcat ttttcaaaa acacaattt 
ttcaacgat gttaaacta ctccgaaag caacgactt 
tcggtcaca agtcgttga agaccagtg gtatcacaa 
ggagaatgt ggatttgcc cctcttgaa cgacctggt 
caacgactc gagcgatct aggccctga aagcgtgct 
caaagactg caagtacaa ccccttact aaacccttt 
cgacatgga atgggtctt cggcttcga catcagcat 
cccagtcct tagcttgac tgcgggcga ccgccgcga 
aatatttta ctcagcgac gaatgtcgg aattgtgta 
caattattg cacaaaaac gatggtcca tctcagagg 
acaccaaag caaaatcgt gagcgtttc agtggaccg 
cagttgtcg ctggctggc caccgctga caacggaaa 
agacaacat tatcatggt gtgggacac tctaaatcg 
ctcggtcgt ttggacgat tttcaacca tccggccga 
aggccacga cttgatcct catcgggct aagtccaag 
cgcccgatt gttaataac aatcaccaa cgaacacga 
aaacacgat taatttttg gctgtggac ctacggcag 
agataaacc ggatgaaac gtacacaga cgaccgcag 
tttaggcgc agccaaagc catcgagtt ccatccaga 
cctggaaga gcacttcat gattgtctt tgaaaaggg 
cgtaatttt cttccagtg ggacttcga acaggacaa 
gttggtcaa aaacgtgag ccccaaaag cccccttag 
caccagaat agggtatta taccgatgc aacctacgt 
taattgttg ccacgaatg tttcgtgag cacctccaa 
aggctgcat cctggtttt tgggaacac gatttttgc 
caaaaacta cgccggaca ggaggatct tgacaacga 
caaaatttt cgtcaagac tatcaagat tgagaaaag 
cgccttgaa ctgtctgac ttcggtcga tgaaatgat 
agtgacggg agattcaaa cggaacaat catgttttt 
cgacaaacg gatcaaact cctctattg gtactcccg 
cagtattga aaagtcgat ccgatcgat gagtttcgt 
gcaaaaccc gcgccgaaa atacatttt tgacgagtc 
ccaaatccg cgatttgga agagagaag tttcgaggg 
aatccccga ttgcatcca agaccgctc gcacgaata 
cgaaatttt gatgaaaca gaaagtgcc aaaagacgc 
gacgctcaa caaccggcc ctttatcac tcgagactt 
tttcatctc caccgtgga cggccaaat ccacatgat 
gaattactc caggcagga gtttatttc gttgttccc 
cactggcga gagcagcgt tgcagcaat tgacgtgca 
tgacgaaaa gccttttgt catagttgc gtacaccaa 
aggtcacct cattatatt caattacga aacgcacga 
agaagtcgt ttcgtcaaa cctgccaga gaccgaacc 
cgaaaccac tataacgag catcaaata ttcgccgca 
aagtttgca cttggcttg tgggcgctc aaatggtga 
actgtggtt gctacaacc ggccactct aggccaaat 
ttacgtgga acctttcag attcagtaa cgacaaagt 
tatgaaaat tgcattctc gcaaaattc gctacaaat 
ggcttatta tgactcgaa taatacaat tttcggctt 
ttactacga ctgtgaaag cgaagactg gatttttaa 
aggaaaagt ccggtctca ctacaaaga cgtttgcaa 
tattttgtt cctggacga agaaccgtt cggccttta 
caccatagg gaaggaccg gcatctgat cagatacac 
gaacattgc agccgagta tttgattta tttaggcca 
agggccgac ttttcttag tgaagagga gctggacat 
ttccacccg cgaccgaat cgagcaaaa ggacactcc 
gatttactt cactcctta tatccggaa aaagttcag 
caaatgtgg ctatttttt gatagccga tgataagca 
caaatataa gacagtcaa tgccaaaac aatgatgtg 
caccgctgt gtacttggg accggccta tggctgttt 
caaagacag tccggtttg caaaatgga atttctccc 
aaagtcgaa tcggaagta tttgatttt catgacgaa 
gaaacaaat tggaattca gttaatgtc gatcgatgg 
caatccgtt taagtttgt tggaagtct ggggcatcc 
tgacgaatt gctagactt ccaaatgtg taaagacgg 
gaaatatgt cttcacgat tggcaaaaa cgacagaag 
tgtgtttat gtggagggt caaaacggc gagtgttga 
aacgatgca cattttggg gggcgtgga gctacagcc 
gtactattg cctcatcga gggcggaag ggccggttt 
cctgtacca ggaaatgcg cgatttgtt ctactacat 
gcaaatcct gcagcaggg agagcaaat ttgtgcgcc 
ccgggtggt cagcaactg cataaacct gggggaatt 
acccgactt aatgcgggc ttgtgggta ctacccctc 
ggaatatga gattgaaaa tctcctaac cgacatgcg 
ttacaaaga atacgacga gaacggcat gctgaagga 
caaagtcac gttttgcga gtttgtcaa actttttat 
taatcataa acccgctta cggttacac aatcgagag 
tcttgagga gtcttttga gacgatcac gacgttttc 
ggacgaata tggggcctc agacatgtc gagggagga 
ctttattga agctataac aacgacagg ggagcaagt 
cagcaacga gcagatttt tagatgctt gattactct 
tttacacga gactgaaac gcctgatca gttcggtga 
gagggactt tagctttat gcccgatac catcgactt 
tgactactt gactgaaga tattttggg catcgacat 
ggaccagat tgaacgact tgaaaaaga tgacgataa 
tgacgatga tgaccgagg aggctacta cgacttctt 
ctatcgctc atgaattgt tattaaaaa catttaaat 
aaaaattta cgtgcttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY