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Gene information
General information
TC034227
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012244,TC012245,TC012243
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid60: 89..9938
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034227
    iBeetle screen iB_03181
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05197
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034227 RA
      MVKIVSSAL KEKDKLLNN NNFQSYSKA MLVAIKDVE 
      TLQLWMDKF HKQLKDLHK AIDDNQKKQ HQTLISSLK 
      PRKKKHFVS KTVIFQKHN FSTMLNYSE SNDFRSQVV 
      EDQWYHKEN VDLPLLNDL VNDSSDLGP ESVLKDCKY 
      NPLLNPFDM EWVFGFDIS IPVLSLTAG DRREIFYSA 
      TNVGIVYNY CTKTMVHLR GHQSKIVSV SVDRSCRWL 
      ATADNGKDN IIMVWDTLN RSVVWTIFN HPAEGHDLI 
      LIGLSPSAR LLITITNEH ENTINFWLW TYGRDKPDE 
      TYTDDRSLG AAKAIEFHP DLEEHFMIV FEKGVIFFQ 
      WDFEQDKLV KNVSPKSPL STRIGYYTD ATYVNCCHE 
      CFVSTSKGC ILVFGNTIF AKNYAGQED LDNDKIFVK 
      TIKIEKSAL NCLTSVDEM IVTGDSNGT IMFFDKRIK 
      LLYWYSRSI EKSIRSMSF VQNPRRKYI FDESQIRDL 
      EERSFEGIP DCIQDRSHE YEILMKQKV PKDATLNNR 
      PFITRDFFI STVDGQIHM MNYSRQEFI SLFPTGESS 
      VAAIDVHDE KPFVIVAYT KGHLIIFNY ETHEEVVSS 
      NLPETEPET TITSIKYSP QSLHLACGR SNGELWLLQ 
      PATLGQIYV EPFRFSNDK VMKIAFSQN SLQMAYYDS 
      NNTIFGFYY DCESEDWIF KGKVRSHYK DVCNILFLD 
      EEPFGLYTI GKDRHLIRY TNIAAEYLI YLGQGPTFL 
      SEEELDIST RDRIEQKDT PIYFTPYIR KKFSKCGYF 
      LIADDKHKY KTVNAKTMM CTAVYLGPA YGCFKDSPV 
      CKMEFLPKS NRKYLIFMT KKQIGIQLM SIDGNPFKF 
      VGSLGHPDE LLDFQMCKD GKYVFTIGK NDRSVFMWR 
      VKTASVETM HILGGVELQ PYYCLIEGG RAGFLYQEM 
      RDLFYYMQI LQQGEQICA PRVVSNCIN LGELPDLMR 
      ACGYYPSEY EIENLLTDM RYKEYDENG MLKDKVTFC 
      EFVKLFINH KPAYGYTIE SLEESFETI TTFSDEYGA 
      SDMSREDFI EAITTTGEQ VSNEQIFRC LITLLHETE 
      TPDQFGERD FSFMPDTID FDYLTEDIL GIDMDQIER 
      LEKDDDNDD DDRGGYYDF FYRS
    CDS sequence
    • >TC034227 RA
      atggtcaaa attgtctca agtgccctt aaagagaag 
      gataagttg ttgaataac aacaatttc cagagttat 
      tcaaaggcc atgctggtt gccatcaaa gacgtcgaa 
      acgttgcag ttgtggatg gataagttt cacaaacaa 
      ttgaaagat ttacataaa gcaatagac gacaatcag 
      aaaaaacag caccagact ttgatttct tcactcaag 
      ccgagaaaa aaaaaacat tttgtttcc aaaactgtc 
      atttttcaa aaacacaat ttttcaacg atgttaaac 
      tactccgaa agcaacgac tttcggtca caagtcgtt 
      gaagaccag tggtatcac aaggagaat gtggatttg 
      cccctcttg aacgacctg gtcaacgac tcgagcgat 
      ctaggccct gaaagcgtg ctcaaagac tgcaagtac 
      aacccctta ctaaaccct ttcgacatg gaatgggtc 
      ttcggcttc gacatcagc atcccagtc cttagcttg 
      actgcgggc gaccgccgc gaaatattt tactcagcg 
      acgaatgtc ggaattgtg tacaattat tgcacaaaa 
      acgatggtc catctcaga ggacaccaa agcaaaatc 
      gtgagcgtt tcagtggac cgcagttgt cgctggctg 
      gccaccgct gacaacgga aaagacaac attatcatg 
      gtgtgggac actctaaat cgctcggtc gtttggacg 
      attttcaac catccggcc gaaggccac gacttgatc 
      ctcatcggg ctaagtcca agcgcccga ttgttaata 
      acaatcacc aacgaacac gaaaacacg attaatttt 
      tggctgtgg acctacggc agagataaa ccggatgaa 
      acgtacaca gacgaccgc agtttaggc gcagccaaa 
      gccatcgag ttccatcca gacctggaa gagcacttc 
      atgattgtc tttgaaaag ggcgtaatt ttcttccag 
      tgggacttc gaacaggac aagttggtc aaaaacgtg 
      agccccaaa agccccctt agcaccaga atagggtat 
      tataccgat gcaacctac gttaattgt tgccacgaa 
      tgtttcgtg agcacctcc aaaggctgc atcctggtt 
      tttgggaac acgattttt gccaaaaac tacgccgga 
      caggaggat cttgacaac gacaaaatt ttcgtcaag 
      actatcaag attgagaaa agcgccttg aactgtctg 
      acttcggtc gatgaaatg atagtgacg ggagattca 
      aacggaaca atcatgttt ttcgacaaa cggatcaaa 
      ctcctctat tggtactcc cgcagtatt gaaaagtcg 
      atccgatcg atgagtttc gtgcaaaac ccgcgccga 
      aaatacatt tttgacgag tcccaaatc cgcgatttg 
      gaagagaga agtttcgag ggaatcccc gattgcatc 
      caagaccgc tcgcacgaa tacgaaatt ttgatgaaa 
      cagaaagtg ccaaaagac gcgacgctc aacaaccgg 
      ccctttatc actcgagac tttttcatc tccaccgtg 
      gacggccaa atccacatg atgaattac tccaggcag 
      gagtttatt tcgttgttc cccactggc gagagcagc 
      gttgcagca attgacgtg catgacgaa aagcctttt 
      gtcatagtt gcgtacacc aaaggtcac ctcattata 
      ttcaattac gaaacgcac gaagaagtc gtttcgtca 
      aacctgcca gagaccgaa cccgaaacc actataacg 
      agcatcaaa tattcgccg caaagtttg cacttggct 
      tgtgggcgc tcaaatggt gaactgtgg ttgctacaa 
      ccggccact ctaggccaa atttacgtg gaacctttc 
      agattcagt aacgacaaa gttatgaaa attgcattc 
      tcgcaaaat tcgctacaa atggcttat tatgactcg 
      aataataca attttcggc ttttactac gactgtgaa 
      agcgaagac tggattttt aaaggaaaa gtccggtct 
      cactacaaa gacgtttgc aatattttg ttcctggac 
      gaagaaccg ttcggcctt tacaccata gggaaggac 
      cggcatctg atcagatac acgaacatt gcagccgag 
      tatttgatt tatttaggc caagggccg acttttctt 
      agtgaagag gagctggac atttccacc cgcgaccga 
      atcgagcaa aaggacact ccgatttac ttcactcct 
      tatatccgg aaaaagttc agcaaatgt ggctatttt 
      ttgatagcc gatgataag cacaaatat aagacagtc 
      aatgccaaa acaatgatg tgcaccgct gtgtacttg 
      ggaccggcc tatggctgt ttcaaagac agtccggtt 
      tgcaaaatg gaatttctc ccaaagtcg aatcggaag 
      tatttgatt ttcatgacg aagaaacaa attggaatt 
      cagttaatg tcgatcgat ggcaatccg tttaagttt 
      gttggaagt ctggggcat cctgacgaa ttgctagac 
      ttccaaatg tgtaaagac gggaaatat gtcttcacg 
      attggcaaa aacgacaga agtgtgttt atgtggagg 
      gtcaaaacg gcgagtgtt gaaacgatg cacattttg 
      gggggcgtg gagctacag ccgtactat tgcctcatc 
      gagggcgga agggccggt ttcctgtac caggaaatg 
      cgcgatttg ttctactac atgcaaatc ctgcagcag 
      ggagagcaa atttgtgcg ccccgggtg gtcagcaac 
      tgcataaac ctgggggaa ttacccgac ttaatgcgg 
      gcttgtggg tactacccc tcggaatat gagattgaa 
      aatctccta accgacatg cgttacaaa gaatacgac 
      gagaacggc atgctgaag gacaaagtc acgttttgc 
      gagtttgtc aaacttttt attaatcat aaacccgct 
      tacggttac acaatcgag agtcttgag gagtctttt 
      gagacgatc acgacgttt tcggacgaa tatggggcc 
      tcagacatg tcgagggag gactttatt gaagctata 
      acaacgaca ggggagcaa gtcagcaac gagcagatt 
      tttagatgc ttgattact cttttacac gagactgaa 
      acgcctgat cagttcggt gagagggac tttagcttt 
      atgcccgat accatcgac tttgactac ttgactgaa 
      gatattttg ggcatcgac atggaccag attgaacga 
      cttgaaaaa gatgacgat aatgacgat gatgaccga 
      ggaggctac tacgacttc ttctatcgc tcatga
    mRNA sequence
    • >TC034227 RA
      tgtgacccc cagagacat ggtcaaaat tgtctcaag 
      tgcccttaa agagaagga taagttgtt gaataacaa 
      caatttcca gagttattc aaaggccat gctggttgc 
      catcaaaga cgtcgaaac gttgcagtt gtggatgga 
      taagtttca caaacaatt gaaagattt acataaagc 
      aatagacga caatcagaa aaaacagca ccagacttt 
      gatttcttc actcaagcc gagaaaaaa aaaacattt 
      tgtttccaa aactgtcat ttttcaaaa acacaattt 
      ttcaacgat gttaaacta ctccgaaag caacgactt 
      tcggtcaca agtcgttga agaccagtg gtatcacaa 
      ggagaatgt ggatttgcc cctcttgaa cgacctggt 
      caacgactc gagcgatct aggccctga aagcgtgct 
      caaagactg caagtacaa ccccttact aaacccttt 
      cgacatgga atgggtctt cggcttcga catcagcat 
      cccagtcct tagcttgac tgcgggcga ccgccgcga 
      aatatttta ctcagcgac gaatgtcgg aattgtgta 
      caattattg cacaaaaac gatggtcca tctcagagg 
      acaccaaag caaaatcgt gagcgtttc agtggaccg 
      cagttgtcg ctggctggc caccgctga caacggaaa 
      agacaacat tatcatggt gtgggacac tctaaatcg 
      ctcggtcgt ttggacgat tttcaacca tccggccga 
      aggccacga cttgatcct catcgggct aagtccaag 
      cgcccgatt gttaataac aatcaccaa cgaacacga 
      aaacacgat taatttttg gctgtggac ctacggcag 
      agataaacc ggatgaaac gtacacaga cgaccgcag 
      tttaggcgc agccaaagc catcgagtt ccatccaga 
      cctggaaga gcacttcat gattgtctt tgaaaaggg 
      cgtaatttt cttccagtg ggacttcga acaggacaa 
      gttggtcaa aaacgtgag ccccaaaag cccccttag 
      caccagaat agggtatta taccgatgc aacctacgt 
      taattgttg ccacgaatg tttcgtgag cacctccaa 
      aggctgcat cctggtttt tgggaacac gatttttgc 
      caaaaacta cgccggaca ggaggatct tgacaacga 
      caaaatttt cgtcaagac tatcaagat tgagaaaag 
      cgccttgaa ctgtctgac ttcggtcga tgaaatgat 
      agtgacggg agattcaaa cggaacaat catgttttt 
      cgacaaacg gatcaaact cctctattg gtactcccg 
      cagtattga aaagtcgat ccgatcgat gagtttcgt 
      gcaaaaccc gcgccgaaa atacatttt tgacgagtc 
      ccaaatccg cgatttgga agagagaag tttcgaggg 
      aatccccga ttgcatcca agaccgctc gcacgaata 
      cgaaatttt gatgaaaca gaaagtgcc aaaagacgc 
      gacgctcaa caaccggcc ctttatcac tcgagactt 
      tttcatctc caccgtgga cggccaaat ccacatgat 
      gaattactc caggcagga gtttatttc gttgttccc 
      cactggcga gagcagcgt tgcagcaat tgacgtgca 
      tgacgaaaa gccttttgt catagttgc gtacaccaa 
      aggtcacct cattatatt caattacga aacgcacga 
      agaagtcgt ttcgtcaaa cctgccaga gaccgaacc 
      cgaaaccac tataacgag catcaaata ttcgccgca 
      aagtttgca cttggcttg tgggcgctc aaatggtga 
      actgtggtt gctacaacc ggccactct aggccaaat 
      ttacgtgga acctttcag attcagtaa cgacaaagt 
      tatgaaaat tgcattctc gcaaaattc gctacaaat 
      ggcttatta tgactcgaa taatacaat tttcggctt 
      ttactacga ctgtgaaag cgaagactg gatttttaa 
      aggaaaagt ccggtctca ctacaaaga cgtttgcaa 
      tattttgtt cctggacga agaaccgtt cggccttta 
      caccatagg gaaggaccg gcatctgat cagatacac 
      gaacattgc agccgagta tttgattta tttaggcca 
      agggccgac ttttcttag tgaagagga gctggacat 
      ttccacccg cgaccgaat cgagcaaaa ggacactcc 
      gatttactt cactcctta tatccggaa aaagttcag 
      caaatgtgg ctatttttt gatagccga tgataagca 
      caaatataa gacagtcaa tgccaaaac aatgatgtg 
      caccgctgt gtacttggg accggccta tggctgttt 
      caaagacag tccggtttg caaaatgga atttctccc 
      aaagtcgaa tcggaagta tttgatttt catgacgaa 
      gaaacaaat tggaattca gttaatgtc gatcgatgg 
      caatccgtt taagtttgt tggaagtct ggggcatcc 
      tgacgaatt gctagactt ccaaatgtg taaagacgg 
      gaaatatgt cttcacgat tggcaaaaa cgacagaag 
      tgtgtttat gtggagggt caaaacggc gagtgttga 
      aacgatgca cattttggg gggcgtgga gctacagcc 
      gtactattg cctcatcga gggcggaag ggccggttt 
      cctgtacca ggaaatgcg cgatttgtt ctactacat 
      gcaaatcct gcagcaggg agagcaaat ttgtgcgcc 
      ccgggtggt cagcaactg cataaacct gggggaatt 
      acccgactt aatgcgggc ttgtgggta ctacccctc 
      ggaatatga gattgaaaa tctcctaac cgacatgcg 
      ttacaaaga atacgacga gaacggcat gctgaagga 
      caaagtcac gttttgcga gtttgtcaa actttttat 
      taatcataa acccgctta cggttacac aatcgagag 
      tcttgagga gtcttttga gacgatcac gacgttttc 
      ggacgaata tggggcctc agacatgtc gagggagga 
      ctttattga agctataac aacgacagg ggagcaagt 
      cagcaacga gcagatttt tagatgctt gattactct 
      tttacacga gactgaaac gcctgatca gttcggtga 
      gagggactt tagctttat gcccgatac catcgactt 
      tgactactt gactgaaga tattttggg catcgacat 
      ggaccagat tgaacgact tgaaaaaga tgacgataa 
      tgacgatga tgaccgagg aggctacta cgacttctt 
      ctatcgctc atgaattgt tattaaaaa catttaaat 
      aaaaattta cgtgcttg