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Gene information
General information
TC001408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1382: 151848..154004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001408
      iBeetle screen iB_03178
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001408 RA
        MAEDGIDLI REILVDVEL SQFLLPIRD DLQITRLEH 
        FDYVKAEDL ENIGLSKPG ARRLLEAVK KKRAQIRKR 
        NLINKLIPV VANKTATLK KTDDSVSLA DFTSCLIPE 
        SNITLSIKL GDGSFGVVR RGEWTSPNG RVLPVAVKI 
        LKADALSQP GVFEDFIKE VQAMHVLSH PNLIRLYGV 
        VLSQPMMMV TELANLGSL LDYLRKQCQ QTPVPMLCE 
        YATQVATGM AYLESKRFL HRDLACRNV LLASADKIK 
        IGDFGLMRA LPQEEDCYV MTEHKKVPF PWCAPESLR 
        SRQFSHASD TWMFGVTVW EMFTFGEDP WMGLIGSEI 
        LRKIDKEGE RLHQPEACP PAVYSILLQ CWLKNPTER 
        PTFAALKEF FRKNKTPVM KAVGGQDEP NKLKIEEGD 
        EIAIIDGSA ELYWWKGQN QRTFEIGLF AR
      CDS sequence
      • >TC001408 RA
        atggccgaa gatggtatt gacttgatc cgcgagata 
        ctagtagat gtagaattg agccagttt ttgttaccc 
        ataagagat gatctccaa ataacgcga ctagaacat 
        ttcgattac gtgaaggcc gaggacttg gaaaacatt 
        ggattaagc aaaccaggt gctagacgg ttgctggaa 
        gccgtcaag aaaaaacgg gcgcagatt aggaaacgc 
        aacttaatc aataagctg atcccggtc gttgctaat 
        aagacagcg actttgaaa aaaactgac gacagtgtg 
        tctttggcg gacttcacc tcttgtctc atccccgaa 
        tctaacata actttgtca attaagctg ggcgatggg 
        tcgtttggg gttgtgcga aggggcgaa tggacgagt 
        cccaacggt cgagtgctt ccagtggct gttaagatc 
        ctcaaagct gatgcgtta tcccaaccg ggggttttc 
        gaggacttc atcaaggaa gtccaagct atgcatgtg 
        ctaagtcac cccaattta atccgtctg tacggcgtg 
        gtgctgtcg cagcccatg atgatggtg accgagtta 
        gcaaatcta gggtctcta ttagactac ctcaggaaa 
        cagtgccaa caaacaccc gtgcccatg ctgtgcgag 
        tatgccacg caagtggcc accgggatg gcgtacctg 
        gagagcaag cggtttctg caccgggac ctcgcctgc 
        cggaacgtc ctgctagcc tcagctgat aagatcaaa 
        atcggtgac tttggcttg atgcgggct ttgccccaa 
        gaggaagac tgttacgtc atgacggag cacaagaag 
        gtgccgttc ccgtggtgc gcccccgag tcgttgcgc 
        tcgaggcag ttcagccac gcgtccgat acgtggatg 
        ttcggggtg acggtgtgg gagatgttc acgttcggg 
        gaggacccg tggatgggg ctcatcggt tcggagatt 
        ttgcggaaa attgataag gagggagag aggctgcac 
        cagccggag gcgtgtccg ccggctgtt tacagtatt 
        ttgctgcag tgttggctc aaaaatccg accgaacgg 
        ccgactttc gctgcgttg aaggagttt ttccggaag 
        aataaaaca ccagtgatg aaggcggtt gggggacag 
        gacgagccg aataaattg aagattgag gaaggggat 
        gagattgcg attattgat gggagtgca gagttgtat 
        tggtggaaa gggcagaat caaaggacg tttgagatc 
        gggttgttt gccaggtga
      mRNA sequence
      • >TC001408 RA
        cctgtcatt gtcaagccc gacttatct tgtggtttt 
        ttaattgtt tatattagt aaaagtaat tgtttaaac 
        aaatttgcg ataaaattt tattgcaaa taaagcata 
        aatggccgt tgaacttta agaatcagt aatgcgtat 
        tacataaaa acactaaaa tggccgaag atggtattg 
        acttgatcc gcgagatac tagtagatg tagaattga 
        gccagtttt tgttaccca taagagatg atctccaaa 
        taacgcgac tagaacatt tcgattacg tgaaggccg 
        aggacttgg aaaacattg gattaagca aaccaggtg 
        ctagacggt tgctggaag ccgtcaaga aaaaacggg 
        cgcagatta ggaaacgca acttaatca ataagctga 
        tcccggtcg ttgctaata agacagcga ctttgaaaa 
        aaactgacg acagtgtgt ctttggcgg acttcacct 
        cttgtctca tccccgaat ctaacataa ctttgtcaa 
        ttaagctgg gcgatgggt cgtttgggg ttgtgcgaa 
        ggggcgaat ggacgagtc ccaacggtc gagtgcttc 
        cagtggctg ttaagatcc tcaaagctg atgcgttat 
        cccaaccgg gggttttcg aggacttca tcaaggaag 
        tccaagcta tgcatgtgc taagtcacc ccaatttaa 
        tccgtctgt acggcgtgg tgctgtcgc agcccatga 
        tgatggtga ccgagttag caaatctag ggtctctat 
        tagactacc tcaggaaac agtgccaac aaacacccg 
        tgcccatgc tgtgcgagt atgccacgc aagtggcca 
        ccgggatgg cgtacctgg agagcaagc ggtttctgc 
        accgggacc tcgcctgcc ggaacgtcc tgctagcct 
        cagctgata agatcaaaa tcggtgact ttggcttga 
        tgcgggctt tgccccaag aggaagact gttacgtca 
        tgacggagc acaagaagg tgccgttcc cgtggtgcg 
        cccccgagt cgttgcgct cgaggcagt tcagccacg 
        cgtccgata cgtggatgt tcggggtga cggtgtggg 
        agatgttca cgttcgggg aggacccgt ggatggggc 
        tcatcggtt cggagattt tgcggaaaa ttgataagg 
        agggagaga ggctgcacc agccggagg cgtgtccgc 
        cggctgttt acagtattt tgctgcagt gttggctca 
        aaaatccga ccgaacggc cgactttcg ctgcgttga 
        aggagtttt tccggaaga ataaaacac cagtgatga 
        aggcggttg ggggacagg acgagccga ataaattga 
        agattgagg aaggggatg agattgcga ttattgatg 
        ggagtgcag agttgtatt ggtggaaag ggcagaatc 
        aaaggacgt ttgagatcg ggttgtttg ccaggtgag 
        aggtttttt ttgacaaag gccggattt tggtaatcg 
        gtttgataa ggcgtgaaa ttggtaatg cgtaagtaa 
        cgggtgaat aagtgggtc aatctaacg attcataaa 
        atttttctt gagtgactt atatgaaat tgaagtgga 
        tatggattt taggacaaa tgcgacccc tgaaattgt 
        tattgaaaa ataaggaac ttttcttta aaaaaatgt 
        ttttgtaag tgattcgta aaatgtttt tgcttgttt 
        ccgttattg tgtttaaag aattccaag agacaagga 
        gatattatc aataaatgt ctttgtgtt tt