Status
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Gene information
General information
TC001384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6922673..6928413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001384
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03177

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late shape irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late structure irregular - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late potentiallyfused - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001384 RA
      MSAAMLTAD WFLLGRDLY FRKFEIYTM GWHQDINLE 
      NFIASSAPY GGPIAIRRD EQKFIKVQG SGQPIISIF 
      SGSGRQITS FKWTKRPIV CMGWSNDEK LICIQEDGV 
      VVLHDMFGK YLHTFVISQ KIQDVKIVD AKIFTSPQN 
      RTGIAVMTS NFKIFLINN IQEPKTRQL SELIKSNLH 
      PTSWVVISE DPHTEVLIA REKELFRLK QDEHHTSLM 
      LEPDISNKY SSILEMAVS FNARHVALF TDSGYLWLG 
      SSNLRTKYC EIDTNIIHK PKQLVWCGN ESVVAYWER 
      DNSLLIVGK HGQKMMYTY DSSVHLSPE IDGVRIISN 
      TQHELLQKV PDVVQKIFR INSTDLGSF LLEASKHYQ 
      KRSHRANEY ICLVKQDLA KAVDQCINA VGYEFDPEV 
      QKMLIRAAQ FGKCFIAYM NSDKYVNII RLLRVLNAV 
      RDPKIGIPL TFTQLQFLG QKVLLDRLI TRKEYFLAL 
      QIAKYLKMP EEEGTSHIL VHWAKYKVG QSHLEEETV 
      AREIAEKLG NTPGISYSE IASTASQFG RKKLAIKLL 
      DYESKASEQ VKLLLELTE NTPALVKAI ESGDTDLVY 
      MVILKLREK MALGDFKMT IRSFPVAQS LYIKYCKEH 
      NTQALNEIY IQEDDFSAQ AQTFIMESL DDKKSHMRD 
      SLLTSAAEA YRKGRKDLN ASLCDDYLK LSRFQRQLD 
      EKSGQQKYT GKSVHETCL LLLKSNEVK LAEKFRNDY 
      KIPDKRFWW LRIQSLAHL EDWTELEKF SKAKKSPIG 
      YAPFVDICL EKNNRHEAL KYLPRVGDD LKVKYYIKA 
      ECLEDAAKI AFEQKDIQS LLYVQTKCP SNSSLSEKV 
      NSFIMQLDN RK
    CDS sequence
    • >TC001384 RA
      atgtcggcg gcaatgcta accgccgac tggttcctc 
      ctgggccga gatttatat tttcgaaaa tttgaaatc 
      tacacaatg ggctggcac caagacatc aatttagag 
      aatttcatt gcgtcgtcg gccccttac gggggcccc 
      atcgcgatc cgccgcgac gaacagaaa ttcatcaaa 
      gtgcaagga agtggccaa cccatcatt tccattttc 
      tcgggatcg ggcaggcaa atcacttcg tttaaatgg 
      accaaacgg cccatcgtg tgcatgggc tggtcaaac 
      gacgagaaa ttgatttgt attcaagag gatggtgtc 
      gtagtttta cacgacatg tttgggaag tatttacac 
      acgtttgta atcagccaa aaaatccaa gatgtgaaa 
      atcgttgac gcgaaaatt tttacaagt ccgcaaaac 
      cggaccgga attgctgtc atgacgtca aattttaaa 
      atcttcctc attaacaac atacaagag cccaaaact 
      agacaactg tcggagcta ataaaatca aatctgcac 
      cccacaagt tgggtcgtc atctctgaa gacccccac 
      accgaggtc ctgatcgct cgcgagaag gaactcttc 
      cgtttgaaa caagatgag caccacacc agtctcatg 
      ctggaacct gatatttcc aacaaatat tcatcgatt 
      ttggaaatg gcagtttcg tttaacgcg cgtcacgtg 
      gcccttttt accgattcg ggttacttg tggttgggg 
      tcgtcaaat ttgcgcact aaatactgc gaaattgat 
      accaacata atccacaaa cccaaacaa ctggtctgg 
      tgtgggaac gaatcagtt gttgcatat tgggagagg 
      gacaattcg cttctaatt gttggtaaa catggacag 
      aaaatgatg tacacttat gatagttcg gtccatttg 
      agtcccgaa attgacggc gtgagaatt atatcaaac 
      acacaacac gaattattg caaaaagtg cccgatgtt 
      gtgcagaaa attttcagg attaacagc accgatctg 
      gggagtttt ctactagaa gcgtcgaaa cactatcag 
      aaacggagc caccgcgca aacgaatac atttgtctg 
      gtgaaacag gacttggca aaagctgtg gatcaatgt 
      atcaacgct gtgggctac gaatttgat ccagaagtc 
      caaaaaatg ttgatcagg gccgcacaa ttcggcaaa 
      tgcttcatt gcatacatg aattccgac aaatatgtg 
      aacataatc cgcctcctg cgtgttttg aatgctgtc 
      agagacccc aaaattggc atcccgctc acattcaca 
      caattgcaa tttttgggc cagaaagtc ctcctcgac 
      cgcctgatc acacgcaag gagtatttt ctcgcgctc 
      caaatcgcc aagtatttg aaaatgccg gaggaagag 
      ggcaccagt cacattctg gtccattgg gccaagtac 
      aaagtgggc caatcacac ctcgaggag gaaacagtg 
      gcccgggaa atagccgaa aagttgggg aatacaccg 
      ggaatttcg tacagtgaa attgccagc actgcttca 
      caattcggg cgcaaaaaa ctcgccatc aagctacta 
      gattatgag tccaaagcg agcgaacaa gtgaaattg 
      ttactagaa ttgaccgaa aacacgcca gctttggtc 
      aaggcgatc gagagtggc gacactgac ttggtctac 
      atggttata ctgaagttg cgcgaaaaa atggcactt 
      ggcgatttt aaaatgacc attcggagc ttccccgta 
      gcacagtcg ctttatatc aaatattgc aaagaacac 
      aacacgcaa gctttgaac gagatttat atacaggag 
      gatgatttt agtgcgcaa gcgcagact ttcatcatg 
      gaaagtctc gacgacaag aagtcgcac atgcgcgac 
      tctttgcta acttcggcc gccgaagcg tatcgaaaa 
      gggcgaaag gacctaaac gcctccctc tgtgacgat 
      tatttgaaa ttatcgcgc ttccagcgg caacttgat 
      gaaaaaagc ggccaacaa aaatacacc ggaaagtct 
      gttcacgaa acgtgtttg ttgctcctt aagagtaac 
      gaagtgaaa ttggcggaa aagttccgc aacgattac 
      aaaatcccc gataagagg ttctggtgg ttgagaatt 
      caaagtttg gcccatctg gaggactgg accgagttg 
      gaaaaattc tcaaaggcg aaaaaatcg cccattggt 
      tacgcgccg ttcgttgat atttgtctc gagaaaaat 
      aaccgacac gaagctttg aagtatttg ccgagagtt 
      ggcgacgat ttgaaagtg aaatactac atcaaggcg 
      gagtgtttg gaggacgcg gccaagata gcgttcgag 
      cagaaggac atccagagc ctgctttat gtccaaacc 
      aagtgtccc tccaactcg agtttatcg gagaaagtg 
      aattcgttc attatgcag ttagataat aggaaataa
    mRNA sequence
    • >TC001384 RA
      cagtggttc attattttt tgtgatttt cataaatta 
      aacaaagaa ttcaacttg aaattgcta attgagctc 
      gaaaatctc gcgaaatgt cggcggcaa tgctaaccg 
      ccgactggt tcctcctgg gccgagatt tatattttc 
      gaaaatttg aaatctaca caatgggct ggcaccaag 
      acatcaatt tagagaatt tcattgcgt cgtcggccc 
      cttacgggg gccccatcg cgatccgcc gcgacgaac 
      agaaattca tcaaagtgc aaggaagtg gccaaccca 
      tcatttcca ttttctcgg gatcgggca ggcaaatca 
      cttcgttta aatggacca aacggccca tcgtgtgca 
      tgggctggt caaacgacg agaaattga tttgtattc 
      aagaggatg gtgtcgtag ttttacacg acatgtttg 
      ggaagtatt tacacacgt ttgtaatca gccaaaaaa 
      tccaagatg tgaaaatcg ttgacgcga aaattttta 
      caagtccgc aaaaccgga ccggaattg ctgtcatga 
      cgtcaaatt ttaaaatct tcctcatta acaacatac 
      aagagccca aaactagac aactgtcgg agctaataa 
      aatcaaatc tgcacccca caagttggg tcgtcatct 
      ctgaagacc cccacaccg aggtcctga tcgctcgcg 
      agaaggaac tcttccgtt tgaaacaag atgagcacc 
      acaccagtc tcatgctgg aacctgata tttccaaca 
      aatattcat cgattttgg aaatggcag tttcgttta 
      acgcgcgtc acgtggccc tttttaccg attcgggtt 
      acttgtggt tggggtcgt caaatttgc gcactaaat 
      actgcgaaa ttgatacca acataatcc acaaaccca 
      aacaactgg tctggtgtg ggaacgaat cagttgttg 
      catattggg agagggaca attcgcttc taattgttg 
      gtaaacatg gacagaaaa tgatgtaca cttatgata 
      gttcggtcc atttgagtc ccgaaattg acggcgtga 
      gaattatat caaacacac aacacgaat tattgcaaa 
      aagtgcccg atgttgtgc agaaaattt tcaggatta 
      acagcaccg atctgggga gttttctac tagaagcgt 
      cgaaacact atcagaaac ggagccacc gcgcaaacg 
      aatacattt gtctggtga aacaggact tggcaaaag 
      ctgtggatc aatgtatca acgctgtgg gctacgaat 
      ttgatccag aagtccaaa aaatgttga tcagggccg 
      cacaattcg gcaaatgct tcattgcat acatgaatt 
      ccgacaaat atgtgaaca taatccgcc tcctgcgtg 
      ttttgaatg ctgtcagag accccaaaa ttggcatcc 
      cgctcacat tcacacaat tgcaatttt tgggccaga 
      aagtcctcc tcgaccgcc tgatcacac gcaaggagt 
      attttctcg cgctccaaa tcgccaagt atttgaaaa 
      tgccggagg aagagggca ccagtcaca ttctggtcc 
      attgggcca agtacaaag tgggccaat cacacctcg 
      aggaggaaa cagtggccc gggaaatag ccgaaaagt 
      tggggaata caccgggaa tttcgtaca gtgaaattg 
      ccagcactg cttcacaat tcgggcgca aaaaactcg 
      ccatcaagc tactagatt atgagtcca aagcgagcg 
      aacaagtga aattgttac tagaattga ccgaaaaca 
      cgccagctt tggtcaagg cgatcgaga gtggcgaca 
      ctgacttgg tctacatgg ttatactga agttgcgcg 
      aaaaaatgg cacttggcg attttaaaa tgaccattc 
      ggagcttcc ccgtagcac agtcgcttt atatcaaat 
      attgcaaag aacacaaca cgcaagctt tgaacgaga 
      tttatatac aggaggatg attttagtg cgcaagcgc 
      agactttca tcatggaaa gtctcgacg acaagaagt 
      cgcacatgc gcgactctt tgctaactt cggccgccg 
      aagcgtatc gaaaagggc gaaaggacc taaacgcct 
      ccctctgtg acgattatt tgaaattat cgcgcttcc 
      agcggcaac ttgatgaaa aaagcggcc aacaaaaat 
      acaccggaa agtctgttc acgaaacgt gtttgttgc 
      tccttaaga gtaacgaag tgaaattgg cggaaaagt 
      tccgcaacg attacaaaa tccccgata agaggttct 
      ggtggttga gaattcaaa gtttggccc atctggagg 
      actggaccg agttggaaa aattctcaa aggcgaaaa 
      aatcgccca ttggttacg cgccgttcg ttgatattt 
      gtctcgaga aaaataacc gacacgaag ctttgaagt 
      atttgccga gagttggcg acgatttga aagtgaaat 
      actacatca aggcggagt gtttggagg acgcggcca 
      agatagcgt tcgagcaga aggacatcc agagcctgc 
      tttatgtcc aaaccaagt gtccctcca actcgagtt 
      tatcggaga aagtgaatt cgttcatta tgcagttag 
      ataatagga aataattgt gatataaac acttagata 
      aataataaa tcgacaact tgagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY