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Gene information
General information
TC001350 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid11: 474814..479728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001350
    iBeetle screen iB_03175
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: All eggs show a slight fluorescence in the posterior part.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001350 RA
      MRSAVCVLV LAFFGCVRA QQLKGNQED PCKLKSKVV 
      GDETYCDRY WECVNGQQE LYDCPNGLV FAGKNRGVT 
      EGCDYPWRS NYCDNKQQA NPPISTEHC DWLYGIFGH 
      ETSCTRYWT CWNGTATEQ LCIGGLLYN ERTHSCDWP 
      ENVDGCQKH PLCNDDPNG NVPLGKSCN RYWQCQGGY 
      PRLQRCPAM LVFDRRSLR CVVPPTEDC DVPSTIPPP 
      PPEEDDDSN IPQNNPKNK QHNFNLPPG AIPVPDKNR 
      PRN
    CDS sequence
    • >TC001350 RA
      atgaggagt gcggtgtgc gttttggtt ttggccttt 
      tttggctgc gtgcgagcc caacagctc aaagggaac 
      caagaggat ccctgcaag ctgaaatcg aaagtggtc 
      ggagacgaa acttattgt gacagatac tgggaatgt 
      gtgaacggc caacaagag ttatacgac tgtcctaat 
      ggtctagtt tttgctgga aaaaatcgc ggagtgact 
      gaaggttgc gactatcct tggaggtcc aactactgc 
      gacaacaaa cagcaagca aacccgccc atttccacc 
      gaacactgt gactggctt tatggcatt ttcgggcac 
      gagacttcc tgtacgagg tactggacg tgctggaat 
      ggcactgcc acggaacag ctttgcatc ggcgggctt 
      ttatacaac gaaagaacg cattcttgt gactggccc 
      gaaaatgta gacgggtgc cagaaacac cctctttgt 
      aatgatgac cccaatggt aatgtcccc ttgggaaaa 
      tcgtgcaat agatactgg cagtgtcaa gggggctac 
      ccccgtttg caaaggtgc ccagctatg ctagtcttc 
      gaccgtagg tctctacgg tgtgttgtg cccccgacc 
      gaagactgc gacgtccca agtactatt ccaccgccg 
      cccccagaa gaggacgac gacagcaac atcccgcaa 
      aacaacccg aaaaataaa caacacaat ttcaattta 
      ccccccggt gctatcccc gtgccggac aaaaacaga 
      ccaaggaat tag
    mRNA sequence
    • >TC001350 RA
      agagtgagg tgtggtggt ggcggccac tttcgaaag 
      gggtaccac tactgtgtc tcttctgtc aaaaccaga 
      agctctgta ctgtggctc ttcagtatc gggtgagaa 
      tccagcgag tttaaagga tttaaagtg tgagtgatt 
      ttaaacatg aggagtgcg gtgtgcgtt ttggttttg 
      gcctttttt ggctgcgtg cgagcccaa cagctcaaa 
      gggaaccaa gaggatccc tgcaagctg aaatcgaaa 
      gtggtcgga gacgaaact tattgtgac agatactgg 
      gaatgtgtg aacggccaa caagagtta tacgactgt 
      cctaatggt ctagttttt gctggaaaa aatcgcgga 
      gtgactgaa ggttgcgac tatccttgg aggtccaac 
      tactgcgac aacaaacag caagcaaac ccgcccatt 
      tccaccgaa cactgtgac tggctttat ggcattttc 
      gggcacgag acttcctgt acgaggtac tggacgtgc 
      tggaatggc actgccacg gaacagctt tgcatcggc 
      gggctttta tacaacgaa agaacgcat tcttgtgac 
      tggcccgaa aatgtagac gggtgccag aaacaccct 
      ctttgtaat gatgacccc aatggtaat gtccccttg 
      ggaaaatcg tgcaataga tactggcag tgtcaaggg 
      ggctacccc cgtttgcaa aggtgccca gctatgcta 
      gtcttcgac cgtaggtct ctacggtgt gttgtgccc 
      ccgaccgaa gactgcgac gtcccaagt actattcca 
      ccgccgccc ccagaagag gacgacgac agcaacatc 
      ccgcaaaac aacccgaaa aataaacaa cacaatttc 
      aatttaccc cccggtgct atccccgtg ccggacaaa 
      aacagacca aggaattag actagttgc cacttttta 
      taatagttt taaagcgag agaatttgt acctgactg 
      gggtacaac ttgttgcca catttgaca ttttcgtta 
      tgcaatgaa aaattaatt tatttccct tatgtgtga 
      atttaattt tactttcac ttgttcaat tacctttct 
      ctacttact ccaacttca tctagtgcc agtattttt 
      atttttccc ttgttgcgt aacacgttg tgaataaat 
      aaatattta agtttgtat gagcgtaat caatacatt 
      t