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Gene information
General information
TC001346 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid11: 448227..452481
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001346
    iBeetle screen iB_03174
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001346 RA
      MNSLKQPHK RIPPVFTEP NNEEFLVPQ APRGQLSQN 
      KSQKFSVLS LSKNKKVQS EKPPENHSG CKTGTKKRL 
      SNEAKIAAD KKCKLEECS LDDDLFDNI DLTQLLNDK 
      TNKSTALST RSASDNSLH KVVDAAQKT PLSLRSKND 
      PNESPKKPL LTQLTQLFD NSQALNQIE TIISESNTN 
      STILREKNW DAKISENLG DVLNENNRD ELESFFDQI 
      EHSVSVMGS DVESVDKDI SKILEGLFG TQKVEQELD 
      KNNENENDI TMSGINASF ATALKQVLL SNITKDVIP 
      VKKAKSETI GEENGTFTE VGPFYGLPL RVKELIKMY 
      KGIDELYDW QDECLNLPA IKEHRNLIY ALPTSGGKT 
      LVAEILMLR EIMCRKKNA IFILPYVAI VQEKVWALS 
      PFAVALDFL VEEYAASKG TYPPRKRRR KNSIYIATI 
      EKALGLLNS LIETDRINE IGLIVVDEL HLVGEPGRG 
      STLEALLTK IMYTNANIQ VVGMSATIG NVNDLCQFL 
      NADIYSQNF RPVELVEYV KCGPEVAKI NYSPKDELL 
      TVVRKIDHK YPSSGMNSL DPDKLGALV MEVVPEGSC 
      LIFCASKKN CENVASLLC KVVNKSLLD YKKEEKEQV 
      KHSLKDEGG SLCKIFNVS IQLGIAYHH SGLTNEERR 
      VIEEAFRAG VISVICCTS TLAAGVNLP AKRVILRQP 
      YVGREFISL SKYKQMVGR AGRAGLGET GESILICNP 
      PELPKVKKL LLSPMDEAL SGMHQNEGK GLRHLLLSC 
      ISLGVANTR AQLQEVAKK TLLHIQSER LEINLKKLT 
      DKAISDLFK LGALQEADL SKKENTIDC DVTVKMETS 
      FSSTDSNSS NVSRKRKIV LKNDTKLVI SKLGCAAIK 
      GNFELARAH QLYDDLHQA QKSLVLLDC LHLLFLVTP 
      YDLAEQIKP DRNDYYNIF RTLGRNELQ TARILGIDD 
      AVALRMLAN QPIRNVPER VLNRFYLTL MLYDLWNET 
      PVFEVSEKF QVNRGIVQH LMTGAATFA SNVVHFCEG 
      LEEFWTFAH LLNGMSQRL SHCCVKELL PLMQLPAVK 
      QSRARQLYN AGYKSIRSI AAANADSLV ESIEYLPRR 
      VAKQLIAAA KMLMLEKVE NLREEAEDV LDGIENPKP 
      FMNQTLLV
    • >TC001346 RB
      MNSLKQPHK RIPPEPNNE EFLVPQAPR GQLSQNKSQ 
      KFSVLSLSK NKKVQSEKP PENHSGCKT GTKKRLSNE 
      AKIAADKKC KLEECSLDD DLFDNIDLT QLLNDKTNK 
      STALSTRSA SDNSLHKVV DAAQKTPLS LRSKNDPNE 
      SPKKPLLTQ LTQLFDNSQ ALNQIETII SESNTNSTI 
      LREKNWDAK ISENLGDVL NENNRDELE SFFDQIEHS 
      VSVMGSDVE SVDKDISKI LEGLFGTQK VEQELDKNN 
      ENENDITMS GINASFATA LKQVLLSNI TKDVIPVKK 
      AKSETIGEE NGTFTEVGP FYGLPLRVK ELIKMYKGI 
      DELYDWQDE CLNLPAIKE HRNLIYALP TSGGKTLVA 
      EILMLREIM CRKKNAIFI LPYVAIVQE KVWALSPFA 
      VALDFLVEE YAASKGTYP PRKRRRKNS IYIATIEKA 
      LGLLNSLIE TDRINEIGL IVVDELHLV GEPGRGSTL 
      EALLTKIMY TNANIQVVG MSATIGNVN DLCQFLNAD 
      IYSQNFRPV ELVEYVKCG PEVAKINYS PKDELLTVV 
      RKIDHKYPS SGMNSLDPD KLGALVMEV VPEGSCLIF 
      CASKKNCEN VASLLCKVV NKSLLDYKK EEKEQVKHS 
      LKDEGGSLC KIFNVSIQL GIAYHHSGL TNEERRVIE 
      EAFRAGVIS VICCTSTLA AGVNLPAKR VILRQPYVG 
      REFISLSKY KQMVGRAGR AGLGETGES ILICNPPEL 
      PKVKKLLLS PMDEALSGM HQNEGKGLR HLLLSCISL 
      GVANTRAQL QEVAKKTLL HIQSERLEI NLKKLTDKA 
      ISDLFKLGA LQEADLSKK ENTIDCDVT VKMETSFSS 
      TDSNSSNVS RKRKIVLKN DTKLVISKL GCAAIKGNF 
      ELARAHQLY DDLHQAQKS LVLLDCLHL LFLVTPYDL 
      AEQIKPDRN DYYNIFRTL GRNELQTAR ILGIDDAVA 
      LRMLANQPI RNVPERVLN RFYLTLMLY DLWNETPVF 
      EVSEKFQVN RGIVQHLMT GAATFASNV VHFCEGLEE 
      FWTFAHLLN GMSQRLSHC CVKELLPLM QLPAVKQSR 
      ARQLYNAGY KSIRSIAAA NADSLVESI EYLPRRVAK 
      QLIAAAKML MLEKVENLR EEAEDVLDG IENPKPFMN 
      QTLLV
    CDS sequence
    • >TC001346 RA
      atgaacagc ttgaagcaa ccccacaaa agaatacct 
      ccagttttt acagagccc aacaatgaa gagttccta 
      gttcctcaa gcaccccga ggacaatta tcccaaaac 
      aaaagtcaa aagttctct gtgctctcg ctttcaaaa 
      aataaaaaa gtacaaagt gagaaacct ccggaaaat 
      catagtggt tgcaaaacc gggactaaa aaaaggttg 
      tctaacgag gcaaaaata gccgctgat aagaaatgc 
      aaactggag gaatgttca cttgatgac gatttattt 
      gataatatt gatctaact cagctacta aatgataaa 
      actaataaa agtacggca ctttcaacg cgctcagcc 
      agcgacaat tccttacat aaagttgtt gatgctgca 
      caaaaaaca ccactgtct ctcagatca aaaaatgac 
      ccaaacgaa tcaccaaaa aaaccatta ctgactcaa 
      ttaactcaa ctgtttgat aacagtcaa gccttgaac 
      cagattgag accataata agtgagagt aacacgaac 
      agcacaatt ttgcgcgag aaaaattgg gatgcaaaa 
      ataagcgag aatttgggg gacgtttta aatgaaaat 
      aatcgcgac gaattggag tcatttttc gaccaaatc 
      gaacattcg gtcagcgta atgggctca gatgttgag 
      tctgtcgat aaagacatt tcgaaaatt ttggaagga 
      cttttcggg acgcaaaaa gtggaacaa gaattggat 
      aaaaataat gagaatgag aatgatatt acaatgtct 
      ggaattaat gcgagtttt gctacggcc ttaaagcaa 
      gtgctttta tcaaacatt actaaagac gtaatacca 
      gtgaaaaaa gccaaaagt gagacgatt ggggaagaa 
      aatggtacg tttaccgaa gttgggcct ttctacggc 
      ttgcctttg agggttaaa gagttgatt aaaatgtac 
      aaaggcatt gatgaattg tacgactgg caggacgag 
      tgtttgaat cttccggcg attaaagag caccggaac 
      cttatctat gcgcttccc acaagtggt ggtaaaact 
      ctagtcgct gagattttg atgctacgg gaaataatg 
      tgtcggaaa aaaaatgcg atatttatc ctaccttac 
      gtggcaatt gttcaagaa aaagtctgg gctttatca 
      ccttttgcc gtggctttg gacttccta gttgaggag 
      tatgccgct agtaaaggc acataccct ccgcggaag 
      cggcgacgg aaaaattca atctatatt gcaacaatt 
      gagaaagct ttggggttg ttgaacagt cttattgag 
      actgatcgt attaacgag attggttta atcgttgtt 
      gatgagctc cacttggtc ggggagcca ggccgtggc 
      tccactttg gaggccctc ctcacaaaa attatgtac 
      actaacgcc aacatacaa gtggttggg atgagcgct 
      acaatcgga aatgtgaac gatttgtgc caattcctc 
      aacgctgat atttattcg caaaatttc cgtcccgtc 
      gaactcgtc gagtatgta aaatgcggg ccagaagtg 
      gcaaaaatt aattacagt ccgaaagac gagttgtta 
      actgtagtt aggaaaatc gaccataaa tatccatca 
      agtggaatg aatagtctt gacccggat aaattaggc 
      gctttagtc atggaagtt gttccagaa ggctcgtgt 
      ttgattttt tgtgcgtct aagaaaaat tgcgaaaac 
      gtggcctcg ctattatgc aaggttgtg aacaaaagt 
      ttgttggat tacaaaaaa gaggaaaag gaacaagtc 
      aaacactcg ttaaaagac gaaggaggt tcattgtgt 
      aaaatattc aacgtgtca attcaatta ggcatcgcg 
      tatcatcat tcaggttta accaatgag gaacgccga 
      gttattgag gaagccttc agggctggt gtcatttca 
      gtcatttgt tgcacgtca actctggca gctggggta 
      aatttaccg gctaaaaga gtcattctg agacaacca 
      tacgttggt cgagagttt atcagtttg tccaaatac 
      aaacaaatg gtaggtcgt gccggtaga gcaggtttg 
      ggggagaca ggcgagagt attttaatt tgtaacccc 
      cccgaatta cccaaagtg aaaaaattg ttattatca 
      ccaatggac gaagcgtta tcagggatg caccaaaac 
      gaagggaaa ggattgaga catttgctc ctgagttgt 
      atcagctta ggtgtcgct aacacacga gcacagttg 
      caagaagtc gcgaaaaaa actttattg cacattcaa 
      agcgaacga cttgaaatc aatttgaaa aaactaaca 
      gataaggcg attagtgac ctttttaaa cttggagcg 
      ttacaagaa gccgatttg agtaaaaag gaaaataca 
      atcgattgt gatgtaacc gttaaaatg gaaacttca 
      ttttcaagc actgattct aacagcagt aatgtatca 
      aggaaaagg aaaatcgtt cttaaaaac gacactaaa 
      cttgtgatc agtaaactg ggttgtgct gcaattaag 
      ggcaatttt gagttggca agagcgcat caactttac 
      gatgacctg caccaagcc cagaaaagt ctagttttg 
      ctcgattgc ctccattta ttgttcctt gtcacaccg 
      tacgacctt gccgagcaa atcaagccg gaccgaaat 
      gactattac aatattttc cgtactttg gggcgcaac 
      gaacttcaa acagctcgt attttagga atcgacgat 
      gctgtggct ttacggatg ttggcaaac caaccaatt 
      agaaatgta cctgaacgt gtcttgaac cgtttttac 
      ctaacttta atgttgtac gacttatgg aacgaaacg 
      cctgttttc gaagtttcg gaaaaattc caagtcaat 
      cgcgggatt gtccagcat ttgatgacc ggagctgcg 
      acgtttgcc tcaaatgtt gtacatttt tgcgaaggt 
      ttggaggag ttttggacg tttgcccat cttttgaac 
      gggatgagt caaagattg tcgcattgt tgtgtgaaa 
      gagttgttg cctttgatg caattaccg gcagttaaa 
      caaagccgg gctaggcag ctttataac gccggttat 
      aagtctata cgtagcata gctgcggca aacgccgat 
      agtttggtt gaaagtatc gaatatttg ccgagaaga 
      gttgcaaaa caactaatt gcagcagct aagatgtta 
      atgttggaa aaagtggag aatttacgg gaggaggct 
      gaggacgta cttgacggt atagaaaac cctaaaccc 
      tttatgaat caaacttta ctagtttaa
    • >TC001346 RB
      atgaacagc ttgaagcaa ccccacaaa agaatacct 
      ccagagccc aacaatgaa gagttccta gttcctcaa 
      gcaccccga ggacaatta tcccaaaac aaaagtcaa 
      aagttctct gtgctctcg ctttcaaaa aataaaaaa 
      gtacaaagt gagaaacct ccggaaaat catagtggt 
      tgcaaaacc gggactaaa aaaaggttg tctaacgag 
      gcaaaaata gccgctgat aagaaatgc aaactggag 
      gaatgttca cttgatgac gatttattt gataatatt 
      gatctaact cagctacta aatgataaa actaataaa 
      agtacggca ctttcaacg cgctcagcc agcgacaat 
      tccttacat aaagttgtt gatgctgca caaaaaaca 
      ccactgtct ctcagatca aaaaatgac ccaaacgaa 
      tcaccaaaa aaaccatta ctgactcaa ttaactcaa 
      ctgtttgat aacagtcaa gccttgaac cagattgag 
      accataata agtgagagt aacacgaac agcacaatt 
      ttgcgcgag aaaaattgg gatgcaaaa ataagcgag 
      aatttgggg gacgtttta aatgaaaat aatcgcgac 
      gaattggag tcatttttc gaccaaatc gaacattcg 
      gtcagcgta atgggctca gatgttgag tctgtcgat 
      aaagacatt tcgaaaatt ttggaagga cttttcggg 
      acgcaaaaa gtggaacaa gaattggat aaaaataat 
      gagaatgag aatgatatt acaatgtct ggaattaat 
      gcgagtttt gctacggcc ttaaagcaa gtgctttta 
      tcaaacatt actaaagac gtaatacca gtgaaaaaa 
      gccaaaagt gagacgatt ggggaagaa aatggtacg 
      tttaccgaa gttgggcct ttctacggc ttgcctttg 
      agggttaaa gagttgatt aaaatgtac aaaggcatt 
      gatgaattg tacgactgg caggacgag tgtttgaat 
      cttccggcg attaaagag caccggaac cttatctat 
      gcgcttccc acaagtggt ggtaaaact ctagtcgct 
      gagattttg atgctacgg gaaataatg tgtcggaaa 
      aaaaatgcg atatttatc ctaccttac gtggcaatt 
      gttcaagaa aaagtctgg gctttatca ccttttgcc 
      gtggctttg gacttccta gttgaggag tatgccgct 
      agtaaaggc acataccct ccgcggaag cggcgacgg 
      aaaaattca atctatatt gcaacaatt gagaaagct 
      ttggggttg ttgaacagt cttattgag actgatcgt 
      attaacgag attggttta atcgttgtt gatgagctc 
      cacttggtc ggggagcca ggccgtggc tccactttg 
      gaggccctc ctcacaaaa attatgtac actaacgcc 
      aacatacaa gtggttggg atgagcgct acaatcgga 
      aatgtgaac gatttgtgc caattcctc aacgctgat 
      atttattcg caaaatttc cgtcccgtc gaactcgtc 
      gagtatgta aaatgcggg ccagaagtg gcaaaaatt 
      aattacagt ccgaaagac gagttgtta actgtagtt 
      aggaaaatc gaccataaa tatccatca agtggaatg 
      aatagtctt gacccggat aaattaggc gctttagtc 
      atggaagtt gttccagaa ggctcgtgt ttgattttt 
      tgtgcgtct aagaaaaat tgcgaaaac gtggcctcg 
      ctattatgc aaggttgtg aacaaaagt ttgttggat 
      tacaaaaaa gaggaaaag gaacaagtc aaacactcg 
      ttaaaagac gaaggaggt tcattgtgt aaaatattc 
      aacgtgtca attcaatta ggcatcgcg tatcatcat 
      tcaggttta accaatgag gaacgccga gttattgag 
      gaagccttc agggctggt gtcatttca gtcatttgt 
      tgcacgtca actctggca gctggggta aatttaccg 
      gctaaaaga gtcattctg agacaacca tacgttggt 
      cgagagttt atcagtttg tccaaatac aaacaaatg 
      gtaggtcgt gccggtaga gcaggtttg ggggagaca 
      ggcgagagt attttaatt tgtaacccc cccgaatta 
      cccaaagtg aaaaaattg ttattatca ccaatggac 
      gaagcgtta tcagggatg caccaaaac gaagggaaa 
      ggattgaga catttgctc ctgagttgt atcagctta 
      ggtgtcgct aacacacga gcacagttg caagaagtc 
      gcgaaaaaa actttattg cacattcaa agcgaacga 
      cttgaaatc aatttgaaa aaactaaca gataaggcg 
      attagtgac ctttttaaa cttggagcg ttacaagaa 
      gccgatttg agtaaaaag gaaaataca atcgattgt 
      gatgtaacc gttaaaatg gaaacttca ttttcaagc 
      actgattct aacagcagt aatgtatca aggaaaagg 
      aaaatcgtt cttaaaaac gacactaaa cttgtgatc 
      agtaaactg ggttgtgct gcaattaag ggcaatttt 
      gagttggca agagcgcat caactttac gatgacctg 
      caccaagcc cagaaaagt ctagttttg ctcgattgc 
      ctccattta ttgttcctt gtcacaccg tacgacctt 
      gccgagcaa atcaagccg gaccgaaat gactattac 
      aatattttc cgtactttg gggcgcaac gaacttcaa 
      acagctcgt attttagga atcgacgat gctgtggct 
      ttacggatg ttggcaaac caaccaatt agaaatgta 
      cctgaacgt gtcttgaac cgtttttac ctaacttta 
      atgttgtac gacttatgg aacgaaacg cctgttttc 
      gaagtttcg gaaaaattc caagtcaat cgcgggatt 
      gtccagcat ttgatgacc ggagctgcg acgtttgcc 
      tcaaatgtt gtacatttt tgcgaaggt ttggaggag 
      ttttggacg tttgcccat cttttgaac gggatgagt 
      caaagattg tcgcattgt tgtgtgaaa gagttgttg 
      cctttgatg caattaccg gcagttaaa caaagccgg 
      gctaggcag ctttataac gccggttat aagtctata 
      cgtagcata gctgcggca aacgccgat agtttggtt 
      gaaagtatc gaatatttg ccgagaaga gttgcaaaa 
      caactaatt gcagcagct aagatgtta atgttggaa 
      aaagtggag aatttacgg gaggaggct gaggacgta 
      cttgacggt atagaaaac cctaaaccc tttatgaat 
      caaacttta ctagtttaa
    mRNA sequence
    • >TC001346 RA
      cggcgcaaa aagaacaat tgtcaagtt caagatttg 
      tctgtcaaa agttatatt tcgaaaatt tgttttatt 
      tgcagtttt gtgtttaat attttatat ttaaaaggt 
      ttaaataaa atagataaa tgtagcaat agaggtgca 
      atttgataa attactaat tttgtgaaa gtgttattg 
      gttaatatt cagggtgat aaaatgaac agcttgaag 
      caaccccac aaaagaata cctccagtt tttacagag 
      cccaacaat gaagagttc ctagttcct caagcaccc 
      cgaggacaa ttatcccaa aacaaaagt caaaagttc 
      tctgtgctc tcgctttca aaaaataaa aaagtacaa 
      agtgagaaa cctccggaa aatcatagt ggttgcaaa 
      accgggact aaaaaaagg ttgtctaac gaggcaaaa 
      atagccgct gataagaaa tgcaaactg gaggaatgt 
      tcacttgat gacgattta tttgataat attgatcta 
      actcagcta ctaaatgat aaaactaat aaaagtacg 
      gcactttca acgcgctca gccagcgac aattcctta 
      cataaagtt gttgatgct gcacaaaaa acaccactg 
      tctctcaga tcaaaaaat gacccaaac gaatcacca 
      aaaaaacca ttactgact caattaact caactgttt 
      gataacagt caagccttg aaccagatt gagaccata 
      ataagtgag agtaacacg aacagcaca attttgcgc 
      gagaaaaat tgggatgca aaaataagc gagaatttg 
      ggggacgtt ttaaatgaa aataatcgc gacgaattg 
      gagtcattt ttcgaccaa atcgaacat tcggtcagc 
      gtaatgggc tcagatgtt gagtctgtc gataaagac 
      atttcgaaa attttggaa ggacttttc gggacgcaa 
      aaagtggaa caagaattg gataaaaat aatgagaat 
      gagaatgat attacaatg tctggaatt aatgcgagt 
      tttgctacg gccttaaag caagtgctt ttatcaaac 
      attactaaa gacgtaata ccagtgaaa aaagccaaa 
      agtgagacg attggggaa gaaaatggt acgtttacc 
      gaagttggg cctttctac ggcttgcct ttgagggtt 
      aaagagttg attaaaatg tacaaaggc attgatgaa 
      ttgtacgac tggcaggac gagtgtttg aatcttccg 
      gcgattaaa gagcaccgg aaccttatc tatgcgctt 
      cccacaagt ggtggtaaa actctagtc gctgagatt 
      ttgatgcta cgggaaata atgtgtcgg aaaaaaaat 
      gcgatattt atcctacct tacgtggca attgttcaa 
      gaaaaagtc tgggcttta tcacctttt gccgtggct 
      ttggacttc ctagttgag gagtatgcc gctagtaaa 
      ggcacatac cctccgcgg aagcggcga cggaaaaat 
      tcaatctat attgcaaca attgagaaa gctttgggg 
      ttgttgaac agtcttatt gagactgat cgtattaac 
      gagattggt ttaatcgtt gttgatgag ctccacttg 
      gtcggggag ccaggccgt ggctccact ttggaggcc 
      ctcctcaca aaaattatg tacactaac gccaacata 
      caagtggtt gggatgagc gctacaatc ggaaatgtg 
      aacgatttg tgccaattc ctcaacgct gatatttat 
      tcgcaaaat ttccgtccc gtcgaactc gtcgagtat 
      gtaaaatgc gggccagaa gtggcaaaa attaattac 
      agtccgaaa gacgagttg ttaactgta gttaggaaa 
      atcgaccat aaatatcca tcaagtgga atgaatagt 
      cttgacccg gataaatta ggcgcttta gtcatggaa 
      gttgttcca gaaggctcg tgtttgatt ttttgtgcg 
      tctaagaaa aattgcgaa aacgtggcc tcgctatta 
      tgcaaggtt gtgaacaaa agtttgttg gattacaaa 
      aaagaggaa aaggaacaa gtcaaacac tcgttaaaa 
      gacgaagga ggttcattg tgtaaaata ttcaacgtg 
      tcaattcaa ttaggcatc gcgtatcat cattcaggt 
      ttaaccaat gaggaacgc cgagttatt gaggaagcc 
      ttcagggct ggtgtcatt tcagtcatt tgttgcacg 
      tcaactctg gcagctggg gtaaattta ccggctaaa 
      agagtcatt ctgagacaa ccatacgtt ggtcgagag 
      tttatcagt ttgtccaaa tacaaacaa atggtaggt 
      cgtgccggt agagcaggt ttgggggag acaggcgag 
      agtatttta atttgtaac ccccccgaa ttacccaaa 
      gtgaaaaaa ttgttatta tcaccaatg gacgaagcg 
      ttatcaggg atgcaccaa aacgaaggg aaaggattg 
      agacatttg ctcctgagt tgtatcagc ttaggtgtc 
      gctaacaca cgagcacag ttgcaagaa gtcgcgaaa 
      aaaacttta ttgcacatt caaagcgaa cgacttgaa 
      atcaatttg aaaaaacta acagataag gcgattagt 
      gaccttttt aaacttgga gcgttacaa gaagccgat 
      ttgagtaaa aaggaaaat acaatcgat tgtgatgta 
      accgttaaa atggaaact tcattttca agcactgat 
      tctaacagc agtaatgta tcaaggaaa aggaaaatc 
      gttcttaaa aacgacact aaacttgtg atcagtaaa 
      ctgggttgt gctgcaatt aagggcaat tttgagttg 
      gcaagagcg catcaactt tacgatgac ctgcaccaa 
      gcccagaaa agtctagtt ttgctcgat tgcctccat 
      ttattgttc cttgtcaca ccgtacgac cttgccgag 
      caaatcaag ccggaccga aatgactat tacaatatt 
      ttccgtact ttggggcgc aacgaactt caaacagct 
      cgtatttta ggaatcgac gatgctgtg gctttacgg 
      atgttggca aaccaacca attagaaat gtacctgaa 
      cgtgtcttg aaccgtttt tacctaact ttaatgttg 
      tacgactta tggaacgaa acgcctgtt ttcgaagtt 
      tcggaaaaa ttccaagtc aatcgcggg attgtccag 
      catttgatg accggagct gcgacgttt gcctcaaat 
      gttgtacat ttttgcgaa ggtttggag gagttttgg 
      acgtttgcc catcttttg aacgggatg agtcaaaga 
      ttgtcgcat tgttgtgtg aaagagttg ttgcctttg 
      atgcaatta ccggcagtt aaacaaagc cgggctagg 
      cagctttat aacgccggt tataagtct atacgtagc 
      atagctgcg gcaaacgcc gatagtttg gttgaaagt 
      atcgaatat ttgccgaga agagttgca aaacaacta 
      attgcagca gctaagatg ttaatgttg gaaaaagtg 
      gagaattta cgggaggag gctgaggac gtacttgac 
      ggtatagaa aaccctaaa ccctttatg aatcaaact 
      ttactagtt taattgtta tcgtttttt gtacaaaca 
      cggatttaa acttaacg
    • >TC001346 RB
      cggcgcaaa aagaacaat tgtcaagtt caagatttg 
      tctgtcaaa agttatatt tcgaaaatt tgttttatt 
      tgcagtttt gtgtttaat attttatat ttaaaaggt 
      ttaaataaa atagataaa tgtagcaat agaggtgca 
      atttgataa attactaat tttgtgaaa gtgttattg 
      gttaatatt cagggtgat aaaatgaac agcttgaag 
      caaccccac aaaagaata cctccagag cccaacaat 
      gaagagttc ctagttcct caagcaccc cgaggacaa 
      ttatcccaa aacaaaagt caaaagttc tctgtgctc 
      tcgctttca aaaaataaa aaagtacaa agtgagaaa 
      cctccggaa aatcatagt ggttgcaaa accgggact 
      aaaaaaagg ttgtctaac gaggcaaaa atagccgct 
      gataagaaa tgcaaactg gaggaatgt tcacttgat 
      gacgattta tttgataat attgatcta actcagcta 
      ctaaatgat aaaactaat aaaagtacg gcactttca 
      acgcgctca gccagcgac aattcctta cataaagtt 
      gttgatgct gcacaaaaa acaccactg tctctcaga 
      tcaaaaaat gacccaaac gaatcacca aaaaaacca 
      ttactgact caattaact caactgttt gataacagt 
      caagccttg aaccagatt gagaccata ataagtgag 
      agtaacacg aacagcaca attttgcgc gagaaaaat 
      tgggatgca aaaataagc gagaatttg ggggacgtt 
      ttaaatgaa aataatcgc gacgaattg gagtcattt 
      ttcgaccaa atcgaacat tcggtcagc gtaatgggc 
      tcagatgtt gagtctgtc gataaagac atttcgaaa 
      attttggaa ggacttttc gggacgcaa aaagtggaa 
      caagaattg gataaaaat aatgagaat gagaatgat 
      attacaatg tctggaatt aatgcgagt tttgctacg 
      gccttaaag caagtgctt ttatcaaac attactaaa 
      gacgtaata ccagtgaaa aaagccaaa agtgagacg 
      attggggaa gaaaatggt acgtttacc gaagttggg 
      cctttctac ggcttgcct ttgagggtt aaagagttg 
      attaaaatg tacaaaggc attgatgaa ttgtacgac 
      tggcaggac gagtgtttg aatcttccg gcgattaaa 
      gagcaccgg aaccttatc tatgcgctt cccacaagt 
      ggtggtaaa actctagtc gctgagatt ttgatgcta 
      cgggaaata atgtgtcgg aaaaaaaat gcgatattt 
      atcctacct tacgtggca attgttcaa gaaaaagtc 
      tgggcttta tcacctttt gccgtggct ttggacttc 
      ctagttgag gagtatgcc gctagtaaa ggcacatac 
      cctccgcgg aagcggcga cggaaaaat tcaatctat 
      attgcaaca attgagaaa gctttgggg ttgttgaac 
      agtcttatt gagactgat cgtattaac gagattggt 
      ttaatcgtt gttgatgag ctccacttg gtcggggag 
      ccaggccgt ggctccact ttggaggcc ctcctcaca 
      aaaattatg tacactaac gccaacata caagtggtt 
      gggatgagc gctacaatc ggaaatgtg aacgatttg 
      tgccaattc ctcaacgct gatatttat tcgcaaaat 
      ttccgtccc gtcgaactc gtcgagtat gtaaaatgc 
      gggccagaa gtggcaaaa attaattac agtccgaaa 
      gacgagttg ttaactgta gttaggaaa atcgaccat 
      aaatatcca tcaagtgga atgaatagt cttgacccg 
      gataaatta ggcgcttta gtcatggaa gttgttcca 
      gaaggctcg tgtttgatt ttttgtgcg tctaagaaa 
      aattgcgaa aacgtggcc tcgctatta tgcaaggtt 
      gtgaacaaa agtttgttg gattacaaa aaagaggaa 
      aaggaacaa gtcaaacac tcgttaaaa gacgaagga 
      ggttcattg tgtaaaata ttcaacgtg tcaattcaa 
      ttaggcatc gcgtatcat cattcaggt ttaaccaat 
      gaggaacgc cgagttatt gaggaagcc ttcagggct 
      ggtgtcatt tcagtcatt tgttgcacg tcaactctg 
      gcagctggg gtaaattta ccggctaaa agagtcatt 
      ctgagacaa ccatacgtt ggtcgagag tttatcagt 
      ttgtccaaa tacaaacaa atggtaggt cgtgccggt 
      agagcaggt ttgggggag acaggcgag agtatttta 
      atttgtaac ccccccgaa ttacccaaa gtgaaaaaa 
      ttgttatta tcaccaatg gacgaagcg ttatcaggg 
      atgcaccaa aacgaaggg aaaggattg agacatttg 
      ctcctgagt tgtatcagc ttaggtgtc gctaacaca 
      cgagcacag ttgcaagaa gtcgcgaaa aaaacttta 
      ttgcacatt caaagcgaa cgacttgaa atcaatttg 
      aaaaaacta acagataag gcgattagt gaccttttt 
      aaacttgga gcgttacaa gaagccgat ttgagtaaa 
      aaggaaaat acaatcgat tgtgatgta accgttaaa 
      atggaaact tcattttca agcactgat tctaacagc 
      agtaatgta tcaaggaaa aggaaaatc gttcttaaa 
      aacgacact aaacttgtg atcagtaaa ctgggttgt 
      gctgcaatt aagggcaat tttgagttg gcaagagcg 
      catcaactt tacgatgac ctgcaccaa gcccagaaa 
      agtctagtt ttgctcgat tgcctccat ttattgttc 
      cttgtcaca ccgtacgac cttgccgag caaatcaag 
      ccggaccga aatgactat tacaatatt ttccgtact 
      ttggggcgc aacgaactt caaacagct cgtatttta 
      ggaatcgac gatgctgtg gctttacgg atgttggca 
      aaccaacca attagaaat gtacctgaa cgtgtcttg 
      aaccgtttt tacctaact ttaatgttg tacgactta 
      tggaacgaa acgcctgtt ttcgaagtt tcggaaaaa 
      ttccaagtc aatcgcggg attgtccag catttgatg 
      accggagct gcgacgttt gcctcaaat gttgtacat 
      ttttgcgaa ggtttggag gagttttgg acgtttgcc 
      catcttttg aacgggatg agtcaaaga ttgtcgcat 
      tgttgtgtg aaagagttg ttgcctttg atgcaatta 
      ccggcagtt aaacaaagc cgggctagg cagctttat 
      aacgccggt tataagtct atacgtagc atagctgcg 
      gcaaacgcc gatagtttg gttgaaagt atcgaatat 
      ttgccgaga agagttgca aaacaacta attgcagca 
      gctaagatg ttaatgttg gaaaaagtg gagaattta 
      cgggaggag gctgaggac gtacttgac ggtatagaa 
      aaccctaaa ccctttatg aatcaaact ttactagtt 
      taattgtta tcgtttttt gtacaaaca cggatttaa 
      acttaacg