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Gene information
General information
TC032260 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14784075..14786297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032260
      iBeetle screen iB_03173

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032260 RA
        MSRLLHTNA KNALYRVLQ PRDLMQTVM 
        KKFGSKSFC FVE
      CDS sequence
      • >TC032260 RA
        atgtcgaga cttttacac acaaatgca 
        aaaaatgca ctatataga gtattacaa 
        ccaagagat ttaatgcag actgtaatg 
        aaaaaattc ggatcaaaa tcgttttgc 
        tttgtcgaa tag
      mRNA sequence
      • >TC032260 RA
        tagcaaaaa taaagaaca gaaataata aaatttctg 
        agatttctg agttaaaat tattatgtg tcactatta 
        ttttgcact atcagtaaa agtggattt agatttaat 
        tactggtga catcaataa ccgactgac caggaacta 
        ataacaagg tttaaaaaa aaaaacagt gagacatgg 
        cagatgaca ttgaaatca acaaactca aactcaaga 
        agttgctgg atgccgaaa atgaagaag aatttatta 
        aatgtatta aagatgatc cttccttaa tcagttgtg 
        aaataccag gtccaccaa caagcgacc taacgttgc 
        tacagtatt gttgtagta agaatttaa tagaagctg 
        ctagatttc tcgtaaaac aaggagcgg atttgacaa 
        aaaatgctg agtatacca tggcacctt tacaaatag 
        catgttttc atggatggc ataaattgc tgaaagcat 
        tattacaag cagaaaacg agtctggcg aagactttg 
        aacttatgg aaggacctt ttgacgaga aaatgaaaa 
        aagcagtgt gtaaagcta ttattaatt ttattacaa 
        attacaaga agaacgacg attatcaaa gttgtctta 
        aaatattaa aaaaaatag ttaaagaga aaaaaatcg 
        atatcgaaa attacatag atgatcacg gccgcaaaa 
        tttctttct acaccatga ctcttggtt tgtatgatc 
        tattatttg aactgtccc taaagatac cattttatc 
        caattgttc caagtgaag tggaaaaat tttttgact 
        tatgcattg acatagata atagctcat cttatgaac 
        cgaatatta ctttcaaat atgacaatt tatgtactg 
        aagaaaact gtacatcaa ttagcgaaa tggactatt 
        tccgaattc ttatttatg acgacaaat ataaaaatt 
        tgttgttgc atccattgg taatgtgtt tcttacttc 
        ttaagtggc ttaaaatta aatatcgtt tttatattc 
        actttgcca tatacatta ttcagatcg cagtgtttg 
        ttaccctca cattcataa atccaactt gtaactatt 
        taaccaatt tgtttaaag ttgctgcga taatgatta 
        gtagtgttg atgtaacat atggtgtaa taatgtgtt 
        ggaaatttt gtaaaaccc agaaggaat ctaaaaact 
        attgggtga taataaaaa tgcatttat atcacatat 
        tgcttcctc tcgcagtca tcatctctg taataattt 
        cccttatat acgaaaaca gcactacaa aaaaacttc 
        tgtttattc cacttttaa ttgcagctt tcaagttta 
        tcttattat ctcgggtaa tcacaattt atggtgaat 
        tattttttg ttgttttgc gtaattgtg ttaaagtct 
        tagtcctga tggttcctt tatttgcta tagtttccg 
        gcatttatt attcaacat ttaacagtt cacctgaaa 
        acaatagta cgaacgatt ttgtgacga cactggtga 
        cggtatttt acaatatgt ttgttttgc ttgttggag 
        agttgcaat tggaaccgt cacaagacg aaccactta 
        atagaacca tttacatgg ttttcattt tactcttca 
        ccatagttt gtgcaaact tgttaaatg ctttagtta 
        tacacgaca caaatcttt taaaaaggg atgctttaa 
        atgtcaatc aaagagaaa tagctcggt acatccatt 
        tatatgaaa tagtcttca agggttttg atgaaacaa 
        attttaaac aaataaaag gtcgtttaa ataaaacta 
        tacaaaatg gaaaacctt cacaatata ttaaactaa 
        accaaaaca taattgaaa gtttaaaca ctttaacaa 
        taaaaacca aaaacattc ctacttttt actgcgata 
        ttgttatac agaatgtcg agactttta cacacaaat 
        gcaaaaaat gcactatat agagtatta caaccaaga 
        gatttaatg cagactgta atgaaaaaa ttcggatca 
        aaatcgttt tgctttgtc gaataggat gtgaatcaa 
        atgtacttg caatcacag ctgtatcaa gtgtttaat 
        atgtaatat aatgtagga tttattgaa tttatttga 
        caataaaaa atttaaagt tca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY