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Gene information
General information
TC001311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14396423..14401683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001311
    iBeetle screen iB_03169

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001311 RA
      MEVMEDGNL MDKVHILIQ RDMEYYKSH QTVLQETIC 
      PGVVGGRLD FPRSASFAA MKLVLWWEE EYVAAYRKS 
      SGFLQKSEV PDQSMDTSG GCGGIVKAS DPTKFVSLI 
      NKSAEQLLE HLHVLTQEA LDHADLTVL TGTIGASAL 
      LKNCLWFYL QSINEIKGS QVELLHESY KKYQEMGEA 
      LAERLLDLH CRLLSLYIL QEAESLDWE NQKPFCESE 
      RGSYVIQMW WLYMQGTRE DLWNTVPPK MAQRVFSGM 
      LNESLTILT VRYTQSCPS EARSKLLVV DISNLLLCI 
      AQLLPSICD KAEELIGLN LNNKSKILR DIHTKCQEL 
      LNCLVLRGA SLDTLQKVF RKGLETVDL FKSRNTGPA 
      SWITFSLPN IFKVQPKNI TRMSDLPPD AAIALELTV 
      LLAQPQPNW SLLLKVLGM KNCKLLKLI LDLSLQNFT 
      IEIQKPVTK SNSCGVFLC SSDGTCKNV DTTVPLLTP 
      LHYYDLIAS ATTVVLGIG NNEDYSDIL FSAISAQPN 
      WAKCFDRRN VWNQIRPPW YESILNLVT PLLSSICKT 
      VVNAVQTGA SMYQAMLII LGSYGQLWD CVDSALPRV 
      ATLIQDILP AEITSLNHS ALVQILISA LYSELLRQS 
      ELPSNKQVT FADSAVSPP AKQTEHKKS VTSFEGTFS 
      SENDVALAV AESLCSIDE DNKHTDQIE EFLEQVKEN 
      LELFEDSVE GSSRVEGSE QVTEVLVSE ILMTTHGKK 
      SLKTLHHFV KNNSEWFFQ KLGVSENSE PVVPNKVTC 
      APCPLLHTV FHIGYRPFD QLLTGAWQP NWIGLLQTP 
      MGLSIDRVW TQISLRWEF RDVNSSSLS TRDAQIITN 
      LTTLLKQT
    CDS sequence
    • >TC001311 RA
      atggaagtg atggaagat ggtaatttg atggacaaa 
      gtccacata ttgatccag cgggacatg gagtattac 
      aagagccac cagaccgta ttacaagaa acaatttgt 
      ccgggggtt gtgggcggc cgcctcgat ttccccaga 
      tcggcctct tttgccgca atgaagctg gttttgtgg 
      tgggaagaa gaatatgtg gcggcctac aggaaaagt 
      tcaggattc ctgcaaaaa tccgaggtt cctgaccag 
      agcatggac acgtctggg ggctgcggc ggcattgtc 
      aaagcctca gatccgacg aaattcgtc agtcttata 
      aataaatcc gctgaacaa cttttggag cacttacat 
      gtgcttact caagaggcg ttagatcac gctgacttg 
      acggtgctt acgggcaca atcggggct tctgcactt 
      ttaaaaaac tgtctctgg ttctatttg caatcaatt 
      aatgagatt aaagggagt caagttgaa ttgttgcac 
      gaaagttac aagaaatac caagagatg ggcgaagcc 
      ctcgctgag cgcttgcta gatttgcat tgtcggttg 
      ttatcactg tacattttg caagaagcc gagtctttg 
      gactgggag aaccagaag cctttctgt gagtcggaa 
      cgggggtcg tacgtgata caaatgtgg tggctttac 
      atgcaaggg accagagag gatttatgg aacactgtt 
      cctccgaaa atggcacaa agggttttt tccgggatg 
      ttaaatgaa tcattaact attttaaca gttcggtac 
      actcagtcg tgtccttct gaagcaagg agtaaacta 
      ctcgtggtt gatattagc aacttacta ttatgcata 
      gcgcaattg cttccaagt atctgtgat aaagcggaa 
      gaactaatt gggttaaac ttaaataac aaaagcaaa 
      atcttacga gacatacac acaaagtgc caggagctg 
      ctgaactgt ttggttttg agaggcgcg tcattggac 
      acacttcaa aaggttttt cgtaaaggc ctagagacc 
      gtggatttg ttcaaatcc cgaaacaca ggcccggcc 
      tcttggatc accttctcc ctccccaac atcttcaaa 
      gtgcagcca aaaaacata acccgaatg tccgactta 
      ccacctgac gccgcaatc gccttagaa ttgaccgtt 
      ttgttggcc caaccccag ccaaactgg tccctcctt 
      ctgaaagtt ctaggaatg aaaaactgc aaactattg 
      aaactcatc ctggaccta tctttgcaa aacttcaca 
      atcgagatc caaaaaccc gtcaccaaa tcaaactct 
      tgcggagtt ttcctctgt tccagcgac ggaacttgc 
      aaaaatgtc gatactacc gtgccccta ctgacccca 
      ctgcattac tacgacttg atcgcatcc gcaacgacc 
      gtggtgttg ggcattggc aacaatgag gattactca 
      gatattcta ttctcggca atcagtgcg cagccaaat 
      tgggcaaag tgtttcgat cgccgcaat gtctggaac 
      caaatccgg ccgccgtgg tacgaatct atcctcaac 
      cttgtcaca cctctgctc tcttccatt tgcaagact 
      gtggtcaat gccgttcaa accggcgcc agcatgtac 
      caggccatg ctcattatt ttgggttct tatggccag 
      ttgtgggac tgcgtggac agtgcgctc ccccgagtg 
      gccaccctg atccaggac attctccca gctgagata 
      acttcccta aatcattcc gctttggtc cagattttg 
      attagtgca ctgtactcg gagctgttg cgtcagtcc 
      gaacttccc tccaataaa caggtcact tttgcggat 
      tcagcggtg tctccaccg gccaaacaa acggagcat 
      aaaaaatcg gtgactagt tttgagggg acgttcagt 
      tctgagaac gacgtagcc ttagctgtg gcagagtcc 
      ttgtgtagt atcgacgag gataacaaa cacacggac 
      caaattgag gagtttttg gaacaagtc aaggaaaac 
      ctggagttg tttgaggat tcggttgag ggttcgtcg 
      agggttgag ggctcggag caagtgact gaagttcta 
      gtcagcgag attttaatg acaactcat gggaaaaaa 
      agcttgaaa actttgcat catttcgta aaaaataac 
      agcgaatgg ttctttcaa aaactcgga gttagtgaa 
      aattctgaa cctgtggta ccgaacaaa gttacttgt 
      gctccgtgt ccactcttg catactgtg tttcatatt 
      ggttaccga cccttcgac cagttgttg actggagca 
      tggcagcct aattggatt ggtttgttg caaacacca 
      atgggatta agtattgat agggtttgg acgcaaata 
      tcactacgc tgggagttt agagatgta aattcttcg 
      agtctgtcg acgagagac gcccagatt atcactaat 
      ttgacgacg ctcttgaaa caaacttga
    mRNA sequence
    • >TC001311 RA
      ctatggaaa caatgtttg gtcgaattt caaataacc 
      ggggtgcaa gtaaattgt atttttttc caccatgga 
      agtgatgga agatggtaa tttgatgga caaagtcca 
      catattgat ccagcggga catggagta ttacaagag 
      ccaccagac cgtattaca agaaacaat ttgtccggg 
      ggttgtggg cggccgcct cgatttccc cagatcggc 
      ctcttttgc cgcaatgaa gctggtttt gtggtggga 
      agaagaata tgtggcggc ctacaggaa aagttcagg 
      attcctgca aaaatccga ggttcctga ccagagcat 
      ggacacgtc tgggggctg cggcggcat tgtcaaagc 
      ctcagatcc gacgaaatt cgtcagtct tataaataa 
      atccgctga acaactttt ggagcactt acatgtgct 
      tactcaaga ggcgttaga tcacgctga cttgacggt 
      gcttacggg cacaatcgg ggcttctgc acttttaaa 
      aaactgtct ctggttcta tttgcaatc aattaatga 
      gattaaagg gagtcaagt tgaattgtt gcacgaaag 
      ttacaagaa ataccaaga gatgggcga agccctcgc 
      tgagcgctt gctagattt gcattgtcg gttgttatc 
      actgtacat tttgcaaga agccgagtc tttggactg 
      ggagaacca gaagccttt ctgtgagtc ggaacgggg 
      gtcgtacgt gatacaaat gtggtggct ttacatgca 
      agggaccag agaggattt atggaacac tgttcctcc 
      gaaaatggc acaaagggt tttttccgg gatgttaaa 
      tgaatcatt aactatttt aacagttcg gtacactca 
      gtcgtgtcc ttctgaagc aaggagtaa actactcgt 
      ggttgatat tagcaactt actattatg catagcgca 
      attgcttcc aagtatctg tgataaagc ggaagaact 
      aattgggtt aaacttaaa taacaaaag caaaatctt 
      acgagacat acacacaaa gtgccagga gctgctgaa 
      ctgtttggt tttgagagg cgcgtcatt ggacacact 
      tcaaaaggt ttttcgtaa aggcctaga gaccgtgga 
      tttgttcaa atcccgaaa cacaggccc ggcctcttg 
      gatcacctt ctccctccc caacatctt caaagtgca 
      gccaaaaaa cataacccg aatgtccga cttaccacc 
      tgacgccgc aatcgcctt agaattgac cgttttgtt 
      ggcccaacc ccagccaaa ctggtccct ccttctgaa 
      agttctagg aatgaaaaa ctgcaaact attgaaact 
      catcctgga cctatcttt gcaaaactt cacaatcga 
      gatccaaaa acccgtcac caaatcaaa ctcttgcgg 
      agttttcct ctgttccag cgacggaac ttgcaaaaa 
      tgtcgatac taccgtgcc cctactgac cccactgca 
      ttactacga cttgatcgc atccgcaac gaccgtggt 
      gttgggcat tggcaacaa tgaggatta ctcagatat 
      tctattctc ggcaatcag tgcgcagcc aaattgggc 
      aaagtgttt cgatcgccg caatgtctg gaaccaaat 
      ccggccgcc gtggtacga atctatcct caaccttgt 
      cacacctct gctctcttc catttgcaa gactgtggt 
      caatgccgt tcaaaccgg cgccagcat gtaccaggc 
      catgctcat tattttggg ttcttatgg ccagttgtg 
      ggactgcgt ggacagtgc gctcccccg agtggccac 
      cctgatcca ggacattct cccagctga gataacttc 
      cctaaatca ttccgcttt ggtccagat tttgattag 
      tgcactgta ctcggagct gttgcgtca gtccgaact 
      tccctccaa taaacaggt cacttttgc ggattcagc 
      ggtgtctcc accggccaa acaaacgga gcataaaaa 
      atcggtgac tagttttga ggggacgtt cagttctga 
      gaacgacgt agccttagc tgtggcaga gtccttgtg 
      tagtatcga cgaggataa caaacacac ggaccaaat 
      tgaggagtt tttggaaca agtcaagga aaacctgga 
      gttgtttga ggattcggt tgagggttc gtcgagggt 
      tgagggctc ggagcaagt gactgaagt tctagtcag 
      cgagatttt aatgacaac tcatgggaa aaaaagctt 
      gaaaacttt gcatcattt cgtaaaaaa taacagcga 
      atggttctt tcaaaaact cggagttag tgaaaattc 
      tgaacctgt ggtaccgaa caaagttac ttgtgctcc 
      gtgtccact cttgcatac tgtgtttca tattggtta 
      ccgaccctt cgaccagtt gttgactgg agcatggca 
      gcctaattg gattggttt gttgcaaac accaatggg 
      attaagtat tgatagggt ttggacgca aatatcact 
      acgctggga gtttagaga tgtaaattc ttcgagtct 
      gtcgacgag agacgccca gattatcac taatttgac 
      gacgctctt gaaacaaac ttgagcctt ttttgtcat 
      gatgtgata attgcatat tttcaatca tttagaaaa 
      gaatgtaaa tattcgttt aggtaagtg gtttacatc 
      cgtttaatc gtacctacc taattaaat taatatccg 
      attcacacc gaattatgt gtaattccc cctaaataa 
      tattacgca catttaatg aatgcatac acaccacaa 
      atgtattga attaagtga gagataatt aaataaact 
      gggttatac gtattagaa attgactgt tcgtacaat 
      gatggaaaa ttaatttcg gtaaatctt gattggaga 
      atacaaaag tttgtaacc cttggcaac tgatgttca 
      atgataatt ttgttttgc taattataa tgacaatga 
      aactgtttt aaacagaat taattaagt actcatttc 
      gtttccaaa tattgggtg aaaacgttt gagcttaaa 
      ttaatagtg aaaacattt catcgtttg cgttttttt 
      attattttg taacgaata tttttatgt aattattac 
      atttaaaca ccgaaaaca atagaaaac gcacactgc 
      taatgagat tctttagat gcaaatagc aattaaatg 
      tgactaatg taaagagac ttgttcacg aatttgaat 
      ttttttgaa attgtaaat aattttgcc tcttactgt 
      atattaata aataaatga atttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY