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Gene information
General information
TC001302 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14199123..14204667
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001302
    iBeetle screen iB_03168

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001302 RA
      MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV 
VDLEHKTPY IIISAARLR VAVAVFART VMPTVLKIY 
YLYQRNQQI VVDNVRHRL LPTLVAFLA NSLSNSLAS 
VQKRITFPL NRRAIKDIQ KDLNPAIDD TTNTTPEES 
VDCQVLFSP KTEILADVI FIHGLHGGI DKTWKQGQW 
RHNGHKLKD QSPVRSQST GNLYVPPRK HSLKRTLSD 
IYNANRTQI KVARKGNEV CVTHQEEWE VIEDVDVDD 
EGCYSNCWP QDWLPKDCP GVRVIALNY TTDVLWCPV 
WKKKRRRTD MVERSDEMI AELCKLGVG KQPIIWVGH 
SKGGLFIKQ IIMNAWERH SQCPEKFAI FQNSKGIMF 
YSVPHKGSV LADFTLPLL RRSVELIEI QRNCDFVLD 
LHKRFLELC KQGHLGADI FSFIETSFT LMSFVYLKI 
VAYESADPD VGIKCDVPL DHREICKPA GRDCFLYLE 
LIKLIKKHS LGGETM
    • >TC001302 RB
      MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV 
VDLEHKTPY IIISAASEW NRQSTANRD VIDLNFWRF 
RAMIPKEMF IRRLRVAVA VFARTVMPT VLKIYYLYQ 
RNQQIVVDN VRHRLLPTL VAFLANSLS NSLASVQKR 
ITFPLNRRA IKDIQKDLN PAIDDTTNT TPEESVDCQ 
VLFSPKTEI LADVIFIHG LHGGIDKTW KQGQWRHNG 
HKLKDQSPV RSQSTGNLY VPPRKHSLK RTLSDIYNA 
NRTQIKVAR KGNEVCVTH QEEWEVIED VDVDDEGCY 
SNCWPQDWL PKDCPGVRV IALNYTTDV LWCPVWKKK 
RRRTDMVER SDEMIAELC KLGVGKQPI IWVGHSKGG 
LFIKQIIMN AWERHSQCP EKFAIFQNS KGIMFYSVP 
HKGSVLADF TLPLLRRSV ELIEIQRNC DFVLDLHKR 
FLELCKQGH LGADIFSFI ETSFTLMSF VYLKIVAYE 
SADPDVGIK CDVPLDHRE ICKPAGRDC FLYLELIKL 
IKKHSLGGE TM
    CDS sequence
    • >TC001302 RA
      atggaatta aatgattca agggatagc ttatcagtg 
gacgattcg ctgctagac gaggtggag gatataatg 
tgcgaccaa acgattatt tccggagag cttagtgtt 
gtagatttg gagcacaag acgccgtat attattata 
tcagcggca cgcctgcgg gtggccgtg gccgtcttc 
gcccgcacc gtaatgccc acagtcctg aaaatctac 
tacctgtac caaagaaac caacaaatc gtggtggac 
aacgtccgc caccgcctc cttccaacc ctagtggcc 
ttcctagcc aacagcctc tcaaactcc ctagcctca 
gtccagaaa cgtattact ttccccctg aaccgtcgt 
gccatcaaa gacatccaa aaagaccta aacccagcc 
attgacgac actacgaac acaacccct gaagaaagc 
gtcgactgc caagtcctc ttctccccc aaaaccgaa 
atcctagcc gacgtcatc ttcatccac ggcctccac 
ggcggcatc gacaagact tggaagcaa ggccagtgg 
cgacacaac ggccacaaa ctcaaagac cagtcgccc 
gtccggagc caatcaacg gggaattta tacgttcca 
ccacgaaaa cattctttg aagcgaacc ctcagcgat 
atttacaac gccaatagg acgcaaatt aaagtcgca 
aggaaggga aatgaggtg tgtgtgacg caccaagag 
gagtgggaa gttatcgaa gatgtggat gtggatgat 
gaggggtgt tattcgaat tgttggccg caggattgg 
ctgccgaag gactgtcca ggggtgagg gtcattgct 
ttgaattac accacggat gtgctgtgg tgtccagtg 
tggaagaaa aagagacga aggactgac atggtcgaa 
aggagcgac gaaatgatc gccgagctt tgcaaatta 
ggggtcggc aaacaaccg attatttgg gtgggtcac 
tctaagggc ggtctcttc attaaacaa atcataatg 
aacgcatgg gagcggcac agccaatgc cccgaaaaa 
ttcgcaatt ttccaaaac agcaaaggc atcatgttc 
tacagcgtg ccgcacaaa gggtcagtc ttagcggac 
ttcactttg ccgcttctt agacgaagc gtcgaactt 
atagaaata caaaggaac tgtgatttc gtactagac 
ctgcacaag cggtttcta gagctgtgc aagcagggg 
catttaggc gccgatatt tttagtttt atcgaaact 
tcgtttact ttaatgtcg ttcgtttat ttgaagatt 
gtcgcgtac gaatctgca gaccccgat gttgggatt 
aaatgcgac gtgcctctg gaccacaga gaaatatgc 
aagcccgca ggacgggac tgcttcctc tacttggag 
ctaatcaag ctgattaaa aaacacagt ttgggtgga 
gaaacaatg tga
    • >TC001302 RB
      atggaatta aatgattca agggatagc ttatcagtg 
gacgattcg ctgctagac gaggtggag gatataatg 
tgcgaccaa acgattatt tccggagag cttagtgtt 
gtagatttg gagcacaag acgccgtat attattata 
tcagcggct tcagaatgg aaccggcaa agtacagcg 
aaccgggat gtgatagac ttgaacttt tggcggttt 
cgtgcaatg ataccaaag gaaatgttc attagacgc 
ctgcgggtg gccgtggcc gtcttcgcc cgcaccgta 
atgcccaca gtcctgaaa atctactac ctgtaccaa 
agaaaccaa caaatcgtg gtggacaac gtccgccac 
cgcctcctt ccaacccta gtggccttc ctagccaac 
agcctctca aactcccta gcctcagtc cagaaacgt 
attactttc cccctgaac cgtcgtgcc atcaaagac 
atccaaaaa gacctaaac ccagccatt gacgacact 
acgaacaca acccctgaa gaaagcgtc gactgccaa 
gtcctcttc tcccccaaa accgaaatc ctagccgac 
gtcatcttc atccacggc ctccacggc ggcatcgac 
aagacttgg aagcaaggc cagtggcga cacaacggc 
cacaaactc aaagaccag tcgcccgtc cggagccaa 
tcaacgggg aatttatac gttccacca cgaaaacat 
tctttgaag cgaaccctc agcgatatt tacaacgcc 
aataggacg caaattaaa gtcgcaagg aagggaaat 
gaggtgtgt gtgacgcac caagaggag tgggaagtt 
atcgaagat gtggatgtg gatgatgag gggtgttat 
tcgaattgt tggccgcag gattggctg ccgaaggac 
tgtccaggg gtgagggtc attgctttg aattacacc 
acggatgtg ctgtggtgt ccagtgtgg aagaaaaag 
agacgaagg actgacatg gtcgaaagg agcgacgaa 
atgatcgcc gagctttgc aaattaggg gtcggcaaa 
caaccgatt atttgggtg ggtcactct aagggcggt 
ctcttcatt aaacaaatc ataatgaac gcatgggag 
cggcacagc caatgcccc gaaaaattc gcaattttc 
caaaacagc aaaggcatc atgttctac agcgtgccg 
cacaaaggg tcagtctta gcggacttc actttgccg 
cttcttaga cgaagcgtc gaacttata gaaatacaa 
aggaactgt gatttcgta ctagacctg cacaagcgg 
tttctagag ctgtgcaag caggggcat ttaggcgcc 
gatattttt agttttatc gaaacttcg tttacttta 
atgtcgttc gtttatttg aagattgtc gcgtacgaa 
tctgcagac cccgatgtt gggattaaa tgcgacgtg 
cctctggac cacagagaa atatgcaag cccgcagga 
cgggactgc ttcctctac ttggagcta atcaagctg 
attaaaaaa cacagtttg ggtggagaa acaatgtga
    mRNA sequence
    • >TC001302 RA
      tattacgtg ataccgcag acggagaaa actacatta 
caacattgt ttccacctg ataatcagt gttgttttt 
agttgtttt aacaatgga attaaatga ttcaaggga 
tagcttatc agtggacga ttcgctgct agacgaggt 
ggaggatat aatgtgcga ccaaacgat tatttccgg 
agagcttag tgttgtaga tttggagca caagacgcc 
gtatattat tatatcagc ggcacgcct gcgggtggc 
cgtggccgt cttcgcccg caccgtaat gcccacagt 
cctgaaaat ctactacct gtaccaaag aaaccaaca 
aatcgtggt ggacaacgt ccgccaccg cctccttcc 
aaccctagt ggccttcct agccaacag cctctcaaa 
ctccctagc ctcagtcca gaaacgtat tactttccc 
cctgaaccg tcgtgccat caaagacat ccaaaaaga 
cctaaaccc agccattga cgacactac gaacacaac 
ccctgaaga aagcgtcga ctgccaagt cctcttctc 
ccccaaaac cgaaatcct agccgacgt catcttcat 
ccacggcct ccacggcgg catcgacaa gacttggaa 
gcaaggcca gtggcgaca caacggcca caaactcaa 
agaccagtc gcccgtccg gagccaatc aacggggaa 
tttatacgt tccaccacg aaaacattc tttgaagcg 
aaccctcag cgatattta caacgccaa taggacgca 
aattaaagt cgcaaggaa gggaaatga ggtgtgtgt 
gacgcacca agaggagtg ggaagttat cgaagatgt 
ggatgtgga tgatgaggg gtgttattc gaattgttg 
gccgcagga ttggctgcc gaaggactg tccaggggt 
gagggtcat tgctttgaa ttacaccac ggatgtgct 
gtggtgtcc agtgtggaa gaaaaagag acgaaggac 
tgacatggt cgaaaggag cgacgaaat gatcgccga 
gctttgcaa attaggggt cggcaaaca accgattat 
ttgggtggg tcactctaa gggcggtct cttcattaa 
acaaatcat aatgaacgc atgggagcg gcacagcca 
atgccccga aaaattcgc aattttcca aaacagcaa 
aggcatcat gttctacag cgtgccgca caaagggtc 
agtcttagc ggacttcac tttgccgct tcttagacg 
aagcgtcga acttataga aatacaaag gaactgtga 
tttcgtact agacctgca caagcggtt tctagagct 
gtgcaagca ggggcattt aggcgccga tatttttag 
ttttatcga aacttcgtt tactttaat gtcgttcgt 
ttatttgaa gattgtcgc gtacgaatc tgcagaccc 
cgatgttgg gattaaatg cgacgtgcc tctggacca 
cagagaaat atgcaagcc cgcaggacg ggactgctt 
cctctactt ggagctaat caagctgat taaaaaaca 
cagtttggg tggagaaac aatgtgatg ctagtttta 
gttagggac tttcaattc tcaggtctt tgttatcat 
tcctgttgt cagtatttg tcggtacca aagtttagg 
tag
    • >TC001302 RB
      tattacgtg ataccgcag acggagaaa actacatta 
caacattgt ttccacctg ataatcagt gttgttttt 
agttgtttt aacaatgga attaaatga ttcaaggga 
tagcttatc agtggacga ttcgctgct agacgaggt 
ggaggatat aatgtgcga ccaaacgat tatttccgg 
agagcttag tgttgtaga tttggagca caagacgcc 
gtatattat tatatcagc ggcttcaga atggaaccg 
gcaaagtac agcgaaccg ggatgtgat agacttgaa 
cttttggcg gtttcgtgc aatgatacc aaaggaaat 
gttcattag acgcctgcg ggtggccgt ggccgtctt 
cgcccgcac cgtaatgcc cacagtcct gaaaatcta 
ctacctgta ccaaagaaa ccaacaaat cgtggtgga 
caacgtccg ccaccgcct ccttccaac cctagtggc 
cttcctagc caacagcct ctcaaactc cctagcctc 
agtccagaa acgtattac tttccccct gaaccgtcg 
tgccatcaa agacatcca aaaagacct aaacccagc 
cattgacga cactacgaa cacaacccc tgaagaaag 
cgtcgactg ccaagtcct cttctcccc caaaaccga 
aatcctagc cgacgtcat cttcatcca cggcctcca 
cggcggcat cgacaagac ttggaagca aggccagtg 
gcgacacaa cggccacaa actcaaaga ccagtcgcc 
cgtccggag ccaatcaac ggggaattt atacgttcc 
accacgaaa acattcttt gaagcgaac cctcagcga 
tatttacaa cgccaatag gacgcaaat taaagtcgc 
aaggaaggg aaatgaggt gtgtgtgac gcaccaaga 
ggagtggga agttatcga agatgtgga tgtggatga 
tgaggggtg ttattcgaa ttgttggcc gcaggattg 
gctgccgaa ggactgtcc aggggtgag ggtcattgc 
tttgaatta caccacgga tgtgctgtg gtgtccagt 
gtggaagaa aaagagacg aaggactga catggtcga 
aaggagcga cgaaatgat cgccgagct ttgcaaatt 
aggggtcgg caaacaacc gattatttg ggtgggtca 
ctctaaggg cggtctctt cattaaaca aatcataat 
gaacgcatg ggagcggca cagccaatg ccccgaaaa 
attcgcaat tttccaaaa cagcaaagg catcatgtt 
ctacagcgt gccgcacaa agggtcagt cttagcgga 
cttcacttt gccgcttct tagacgaag cgtcgaact 
tatagaaat acaaaggaa ctgtgattt cgtactaga 
cctgcacaa gcggtttct agagctgtg caagcaggg 
gcatttagg cgccgatat ttttagttt tatcgaaac 
ttcgtttac tttaatgtc gttcgttta tttgaagat 
tgtcgcgta cgaatctgc agaccccga tgttgggat 
taaatgcga cgtgcctct ggaccacag agaaatatg 
caagcccgc aggacggga ctgcttcct ctacttgga 
gctaatcaa gctgattaa aaaacacag tttgggtgg 
agaaacaat gtgatgcta gttttagtt agggacttt 
caattctca ggtctttgt tatcattcc tgttgtcag 
tatttgtcg gtaccaaag tttaggtag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY