Status
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Gene information
General information
TC001302 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14199123..14204667
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001302
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03168

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001302 RA
      MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV 
      VDLEHKTPY IIISAARLR VAVAVFART VMPTVLKIY 
      YLYQRNQQI VVDNVRHRL LPTLVAFLA NSLSNSLAS 
      VQKRITFPL NRRAIKDIQ KDLNPAIDD TTNTTPEES 
      VDCQVLFSP KTEILADVI FIHGLHGGI DKTWKQGQW 
      RHNGHKLKD QSPVRSQST GNLYVPPRK HSLKRTLSD 
      IYNANRTQI KVARKGNEV CVTHQEEWE VIEDVDVDD 
      EGCYSNCWP QDWLPKDCP GVRVIALNY TTDVLWCPV 
      WKKKRRRTD MVERSDEMI AELCKLGVG KQPIIWVGH 
      SKGGLFIKQ IIMNAWERH SQCPEKFAI FQNSKGIMF 
      YSVPHKGSV LADFTLPLL RRSVELIEI QRNCDFVLD 
      LHKRFLELC KQGHLGADI FSFIETSFT LMSFVYLKI 
      VAYESADPD VGIKCDVPL DHREICKPA GRDCFLYLE 
      LIKLIKKHS LGGETM
    • >TC001302 RB
      MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV 
      VDLEHKTPY IIISAASEW NRQSTANRD VIDLNFWRF 
      RAMIPKEMF IRRLRVAVA VFARTVMPT VLKIYYLYQ 
      RNQQIVVDN VRHRLLPTL VAFLANSLS NSLASVQKR 
      ITFPLNRRA IKDIQKDLN PAIDDTTNT TPEESVDCQ 
      VLFSPKTEI LADVIFIHG LHGGIDKTW KQGQWRHNG 
      HKLKDQSPV RSQSTGNLY VPPRKHSLK RTLSDIYNA 
      NRTQIKVAR KGNEVCVTH QEEWEVIED VDVDDEGCY 
      SNCWPQDWL PKDCPGVRV IALNYTTDV LWCPVWKKK 
      RRRTDMVER SDEMIAELC KLGVGKQPI IWVGHSKGG 
      LFIKQIIMN AWERHSQCP EKFAIFQNS KGIMFYSVP 
      HKGSVLADF TLPLLRRSV ELIEIQRNC DFVLDLHKR 
      FLELCKQGH LGADIFSFI ETSFTLMSF VYLKIVAYE 
      SADPDVGIK CDVPLDHRE ICKPAGRDC FLYLELIKL 
      IKKHSLGGE TM
    CDS sequence
    • >TC001302 RA
      atggaatta aatgattca agggatagc ttatcagtg 
      gacgattcg ctgctagac gaggtggag gatataatg 
      tgcgaccaa acgattatt tccggagag cttagtgtt 
      gtagatttg gagcacaag acgccgtat attattata 
      tcagcggca cgcctgcgg gtggccgtg gccgtcttc 
      gcccgcacc gtaatgccc acagtcctg aaaatctac 
      tacctgtac caaagaaac caacaaatc gtggtggac 
      aacgtccgc caccgcctc cttccaacc ctagtggcc 
      ttcctagcc aacagcctc tcaaactcc ctagcctca 
      gtccagaaa cgtattact ttccccctg aaccgtcgt 
      gccatcaaa gacatccaa aaagaccta aacccagcc 
      attgacgac actacgaac acaacccct gaagaaagc 
      gtcgactgc caagtcctc ttctccccc aaaaccgaa 
      atcctagcc gacgtcatc ttcatccac ggcctccac 
      ggcggcatc gacaagact tggaagcaa ggccagtgg 
      cgacacaac ggccacaaa ctcaaagac cagtcgccc 
      gtccggagc caatcaacg gggaattta tacgttcca 
      ccacgaaaa cattctttg aagcgaacc ctcagcgat 
      atttacaac gccaatagg acgcaaatt aaagtcgca 
      aggaaggga aatgaggtg tgtgtgacg caccaagag 
      gagtgggaa gttatcgaa gatgtggat gtggatgat 
      gaggggtgt tattcgaat tgttggccg caggattgg 
      ctgccgaag gactgtcca ggggtgagg gtcattgct 
      ttgaattac accacggat gtgctgtgg tgtccagtg 
      tggaagaaa aagagacga aggactgac atggtcgaa 
      aggagcgac gaaatgatc gccgagctt tgcaaatta 
      ggggtcggc aaacaaccg attatttgg gtgggtcac 
      tctaagggc ggtctcttc attaaacaa atcataatg 
      aacgcatgg gagcggcac agccaatgc cccgaaaaa 
      ttcgcaatt ttccaaaac agcaaaggc atcatgttc 
      tacagcgtg ccgcacaaa gggtcagtc ttagcggac 
      ttcactttg ccgcttctt agacgaagc gtcgaactt 
      atagaaata caaaggaac tgtgatttc gtactagac 
      ctgcacaag cggtttcta gagctgtgc aagcagggg 
      catttaggc gccgatatt tttagtttt atcgaaact 
      tcgtttact ttaatgtcg ttcgtttat ttgaagatt 
      gtcgcgtac gaatctgca gaccccgat gttgggatt 
      aaatgcgac gtgcctctg gaccacaga gaaatatgc 
      aagcccgca ggacgggac tgcttcctc tacttggag 
      ctaatcaag ctgattaaa aaacacagt ttgggtgga 
      gaaacaatg tga
    • >TC001302 RB
      atggaatta aatgattca agggatagc ttatcagtg 
      gacgattcg ctgctagac gaggtggag gatataatg 
      tgcgaccaa acgattatt tccggagag cttagtgtt 
      gtagatttg gagcacaag acgccgtat attattata 
      tcagcggct tcagaatgg aaccggcaa agtacagcg 
      aaccgggat gtgatagac ttgaacttt tggcggttt 
      cgtgcaatg ataccaaag gaaatgttc attagacgc 
      ctgcgggtg gccgtggcc gtcttcgcc cgcaccgta 
      atgcccaca gtcctgaaa atctactac ctgtaccaa 
      agaaaccaa caaatcgtg gtggacaac gtccgccac 
      cgcctcctt ccaacccta gtggccttc ctagccaac 
      agcctctca aactcccta gcctcagtc cagaaacgt 
      attactttc cccctgaac cgtcgtgcc atcaaagac 
      atccaaaaa gacctaaac ccagccatt gacgacact 
      acgaacaca acccctgaa gaaagcgtc gactgccaa 
      gtcctcttc tcccccaaa accgaaatc ctagccgac 
      gtcatcttc atccacggc ctccacggc ggcatcgac 
      aagacttgg aagcaaggc cagtggcga cacaacggc 
      cacaaactc aaagaccag tcgcccgtc cggagccaa 
      tcaacgggg aatttatac gttccacca cgaaaacat 
      tctttgaag cgaaccctc agcgatatt tacaacgcc 
      aataggacg caaattaaa gtcgcaagg aagggaaat 
      gaggtgtgt gtgacgcac caagaggag tgggaagtt 
      atcgaagat gtggatgtg gatgatgag gggtgttat 
      tcgaattgt tggccgcag gattggctg ccgaaggac 
      tgtccaggg gtgagggtc attgctttg aattacacc 
      acggatgtg ctgtggtgt ccagtgtgg aagaaaaag 
      agacgaagg actgacatg gtcgaaagg agcgacgaa 
      atgatcgcc gagctttgc aaattaggg gtcggcaaa 
      caaccgatt atttgggtg ggtcactct aagggcggt 
      ctcttcatt aaacaaatc ataatgaac gcatgggag 
      cggcacagc caatgcccc gaaaaattc gcaattttc 
      caaaacagc aaaggcatc atgttctac agcgtgccg 
      cacaaaggg tcagtctta gcggacttc actttgccg 
      cttcttaga cgaagcgtc gaacttata gaaatacaa 
      aggaactgt gatttcgta ctagacctg cacaagcgg 
      tttctagag ctgtgcaag caggggcat ttaggcgcc 
      gatattttt agttttatc gaaacttcg tttacttta 
      atgtcgttc gtttatttg aagattgtc gcgtacgaa 
      tctgcagac cccgatgtt gggattaaa tgcgacgtg 
      cctctggac cacagagaa atatgcaag cccgcagga 
      cgggactgc ttcctctac ttggagcta atcaagctg 
      attaaaaaa cacagtttg ggtggagaa acaatgtga
    mRNA sequence
    • >TC001302 RA
      tattacgtg ataccgcag acggagaaa actacatta 
      caacattgt ttccacctg ataatcagt gttgttttt 
      agttgtttt aacaatgga attaaatga ttcaaggga 
      tagcttatc agtggacga ttcgctgct agacgaggt 
      ggaggatat aatgtgcga ccaaacgat tatttccgg 
      agagcttag tgttgtaga tttggagca caagacgcc 
      gtatattat tatatcagc ggcacgcct gcgggtggc 
      cgtggccgt cttcgcccg caccgtaat gcccacagt 
      cctgaaaat ctactacct gtaccaaag aaaccaaca 
      aatcgtggt ggacaacgt ccgccaccg cctccttcc 
      aaccctagt ggccttcct agccaacag cctctcaaa 
      ctccctagc ctcagtcca gaaacgtat tactttccc 
      cctgaaccg tcgtgccat caaagacat ccaaaaaga 
      cctaaaccc agccattga cgacactac gaacacaac 
      ccctgaaga aagcgtcga ctgccaagt cctcttctc 
      ccccaaaac cgaaatcct agccgacgt catcttcat 
      ccacggcct ccacggcgg catcgacaa gacttggaa 
      gcaaggcca gtggcgaca caacggcca caaactcaa 
      agaccagtc gcccgtccg gagccaatc aacggggaa 
      tttatacgt tccaccacg aaaacattc tttgaagcg 
      aaccctcag cgatattta caacgccaa taggacgca 
      aattaaagt cgcaaggaa gggaaatga ggtgtgtgt 
      gacgcacca agaggagtg ggaagttat cgaagatgt 
      ggatgtgga tgatgaggg gtgttattc gaattgttg 
      gccgcagga ttggctgcc gaaggactg tccaggggt 
      gagggtcat tgctttgaa ttacaccac ggatgtgct 
      gtggtgtcc agtgtggaa gaaaaagag acgaaggac 
      tgacatggt cgaaaggag cgacgaaat gatcgccga 
      gctttgcaa attaggggt cggcaaaca accgattat 
      ttgggtggg tcactctaa gggcggtct cttcattaa 
      acaaatcat aatgaacgc atgggagcg gcacagcca 
      atgccccga aaaattcgc aattttcca aaacagcaa 
      aggcatcat gttctacag cgtgccgca caaagggtc 
      agtcttagc ggacttcac tttgccgct tcttagacg 
      aagcgtcga acttataga aatacaaag gaactgtga 
      tttcgtact agacctgca caagcggtt tctagagct 
      gtgcaagca ggggcattt aggcgccga tatttttag 
      ttttatcga aacttcgtt tactttaat gtcgttcgt 
      ttatttgaa gattgtcgc gtacgaatc tgcagaccc 
      cgatgttgg gattaaatg cgacgtgcc tctggacca 
      cagagaaat atgcaagcc cgcaggacg ggactgctt 
      cctctactt ggagctaat caagctgat taaaaaaca 
      cagtttggg tggagaaac aatgtgatg ctagtttta 
      gttagggac tttcaattc tcaggtctt tgttatcat 
      tcctgttgt cagtatttg tcggtacca aagtttagg 
      tag
    • >TC001302 RB
      tattacgtg ataccgcag acggagaaa actacatta 
      caacattgt ttccacctg ataatcagt gttgttttt 
      agttgtttt aacaatgga attaaatga ttcaaggga 
      tagcttatc agtggacga ttcgctgct agacgaggt 
      ggaggatat aatgtgcga ccaaacgat tatttccgg 
      agagcttag tgttgtaga tttggagca caagacgcc 
      gtatattat tatatcagc ggcttcaga atggaaccg 
      gcaaagtac agcgaaccg ggatgtgat agacttgaa 
      cttttggcg gtttcgtgc aatgatacc aaaggaaat 
      gttcattag acgcctgcg ggtggccgt ggccgtctt 
      cgcccgcac cgtaatgcc cacagtcct gaaaatcta 
      ctacctgta ccaaagaaa ccaacaaat cgtggtgga 
      caacgtccg ccaccgcct ccttccaac cctagtggc 
      cttcctagc caacagcct ctcaaactc cctagcctc 
      agtccagaa acgtattac tttccccct gaaccgtcg 
      tgccatcaa agacatcca aaaagacct aaacccagc 
      cattgacga cactacgaa cacaacccc tgaagaaag 
      cgtcgactg ccaagtcct cttctcccc caaaaccga 
      aatcctagc cgacgtcat cttcatcca cggcctcca 
      cggcggcat cgacaagac ttggaagca aggccagtg 
      gcgacacaa cggccacaa actcaaaga ccagtcgcc 
      cgtccggag ccaatcaac ggggaattt atacgttcc 
      accacgaaa acattcttt gaagcgaac cctcagcga 
      tatttacaa cgccaatag gacgcaaat taaagtcgc 
      aaggaaggg aaatgaggt gtgtgtgac gcaccaaga 
      ggagtggga agttatcga agatgtgga tgtggatga 
      tgaggggtg ttattcgaa ttgttggcc gcaggattg 
      gctgccgaa ggactgtcc aggggtgag ggtcattgc 
      tttgaatta caccacgga tgtgctgtg gtgtccagt 
      gtggaagaa aaagagacg aaggactga catggtcga 
      aaggagcga cgaaatgat cgccgagct ttgcaaatt 
      aggggtcgg caaacaacc gattatttg ggtgggtca 
      ctctaaggg cggtctctt cattaaaca aatcataat 
      gaacgcatg ggagcggca cagccaatg ccccgaaaa 
      attcgcaat tttccaaaa cagcaaagg catcatgtt 
      ctacagcgt gccgcacaa agggtcagt cttagcgga 
      cttcacttt gccgcttct tagacgaag cgtcgaact 
      tatagaaat acaaaggaa ctgtgattt cgtactaga 
      cctgcacaa gcggtttct agagctgtg caagcaggg 
      gcatttagg cgccgatat ttttagttt tatcgaaac 
      ttcgtttac tttaatgtc gttcgttta tttgaagat 
      tgtcgcgta cgaatctgc agaccccga tgttgggat 
      taaatgcga cgtgcctct ggaccacag agaaatatg 
      caagcccgc aggacggga ctgcttcct ctacttgga 
      gctaatcaa gctgattaa aaaacacag tttgggtgg 
      agaaacaat gtgatgcta gttttagtt agggacttt 
      caattctca ggtctttgt tatcattcc tgttgtcag 
      tatttgtcg gtaccaaag tttaggtag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY