| General information | |||
| TC001302 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid9: 222338..227882 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001302 | |
| General information | ||||
| iB_03168 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001302 RA
MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV VDLEHKTPY IIISAARLR VAVAVFART VMPTVLKIY YLYQRNQQI VVDNVRHRL LPTLVAFLA NSLSNSLAS VQKRITFPL NRRAIKDIQ KDLNPAIDD TTNTTPEES VDCQVLFSP KTEILADVI FIHGLHGGI DKTWKQGQW RHNGHKLKD QSPVRSQST GNLYVPPRK HSLKRTLSD IYNANRTQI KVARKGNEV CVTHQEEWE VIEDVDVDD EGCYSNCWP QDWLPKDCP GVRVIALNY TTDVLWCPV WKKKRRRTD MVERSDEMI AELCKLGVG KQPIIWVGH SKGGLFIKQ IIMNAWERH SQCPEKFAI FQNSKGIMF YSVPHKGSV LADFTLPLL RRSVELIEI QRNCDFVLD LHKRFLELC KQGHLGADI FSFIETSFT LMSFVYLKI VAYESADPD VGIKCDVPL DHREICKPA GRDCFLYLE LIKLIKKHS LGGETM
- >TC001302 RB
MELNDSRDS LSVDDSLLD EVEDIMCDQ TIISGELSV VDLEHKTPY IIISAASEW NRQSTANRD VIDLNFWRF RAMIPKEMF IRRLRVAVA VFARTVMPT VLKIYYLYQ RNQQIVVDN VRHRLLPTL VAFLANSLS NSLASVQKR ITFPLNRRA IKDIQKDLN PAIDDTTNT TPEESVDCQ VLFSPKTEI LADVIFIHG LHGGIDKTW KQGQWRHNG HKLKDQSPV RSQSTGNLY VPPRKHSLK RTLSDIYNA NRTQIKVAR KGNEVCVTH QEEWEVIED VDVDDEGCY SNCWPQDWL PKDCPGVRV IALNYTTDV LWCPVWKKK RRRTDMVER SDEMIAELC KLGVGKQPI IWVGHSKGG LFIKQIIMN AWERHSQCP EKFAIFQNS KGIMFYSVP HKGSVLADF TLPLLRRSV ELIEIQRNC DFVLDLHKR FLELCKQGH LGADIFSFI ETSFTLMSF VYLKIVAYE SADPDVGIK CDVPLDHRE ICKPAGRDC FLYLELIKL IKKHSLGGE TM
- >TC001302 RA
atggaatta aatgattca agggatagc ttatcagtg gacgattcg ctgctagac gaggtggag gatataatg tgcgaccaa acgattatt tccggagag cttagtgtt gtagatttg gagcacaag acgccgtat attattata tcagcggca cgcctgcgg gtggccgtg gccgtcttc gcccgcacc gtaatgccc acagtcctg aaaatctac tacctgtac caaagaaac caacaaatc gtggtggac aacgtccgc caccgcctc cttccaacc ctagtggcc ttcctagcc aacagcctc tcaaactcc ctagcctca gtccagaaa cgtattact ttccccctg aaccgtcgt gccatcaaa gacatccaa aaagaccta aacccagcc attgacgac actacgaac acaacccct gaagaaagc gtcgactgc caagtcctc ttctccccc aaaaccgaa atcctagcc gacgtcatc ttcatccac ggcctccac ggcggcatc gacaagact tggaagcaa ggccagtgg cgacacaac ggccacaaa ctcaaagac cagtcgccc gtccggagc caatcaacg gggaattta tacgttcca ccacgaaaa cattctttg aagcgaacc ctcagcgat atttacaac gccaatagg acgcaaatt aaagtcgca aggaaggga aatgaggtg tgtgtgacg caccaagag gagtgggaa gttatcgaa gatgtggat gtggatgat gaggggtgt tattcgaat tgttggccg caggattgg ctgccgaag gactgtcca ggggtgagg gtcattgct ttgaattac accacggat gtgctgtgg tgtccagtg tggaagaaa aagagacga aggactgac atggtcgaa aggagcgac gaaatgatc gccgagctt tgcaaatta ggggtcggc aaacaaccg attatttgg gtgggtcac tctaagggc ggtctcttc attaaacaa atcataatg aacgcatgg gagcggcac agccaatgc cccgaaaaa ttcgcaatt ttccaaaac agcaaaggc atcatgttc tacagcgtg ccgcacaaa gggtcagtc ttagcggac ttcactttg ccgcttctt agacgaagc gtcgaactt atagaaata caaaggaac tgtgatttc gtactagac ctgcacaag cggtttcta gagctgtgc aagcagggg catttaggc gccgatatt tttagtttt atcgaaact tcgtttact ttaatgtcg ttcgtttat ttgaagatt gtcgcgtac gaatctgca gaccccgat gttgggatt aaatgcgac gtgcctctg gaccacaga gaaatatgc aagcccgca ggacgggac tgcttcctc tacttggag ctaatcaag ctgattaaa aaacacagt ttgggtgga gaaacaatg tga
- >TC001302 RB
atggaatta aatgattca agggatagc ttatcagtg gacgattcg ctgctagac gaggtggag gatataatg tgcgaccaa acgattatt tccggagag cttagtgtt gtagatttg gagcacaag acgccgtat attattata tcagcggct tcagaatgg aaccggcaa agtacagcg aaccgggat gtgatagac ttgaacttt tggcggttt cgtgcaatg ataccaaag gaaatgttc attagacgc ctgcgggtg gccgtggcc gtcttcgcc cgcaccgta atgcccaca gtcctgaaa atctactac ctgtaccaa agaaaccaa caaatcgtg gtggacaac gtccgccac cgcctcctt ccaacccta gtggccttc ctagccaac agcctctca aactcccta gcctcagtc cagaaacgt attactttc cccctgaac cgtcgtgcc atcaaagac atccaaaaa gacctaaac ccagccatt gacgacact acgaacaca acccctgaa gaaagcgtc gactgccaa gtcctcttc tcccccaaa accgaaatc ctagccgac gtcatcttc atccacggc ctccacggc ggcatcgac aagacttgg aagcaaggc cagtggcga cacaacggc cacaaactc aaagaccag tcgcccgtc cggagccaa tcaacgggg aatttatac gttccacca cgaaaacat tctttgaag cgaaccctc agcgatatt tacaacgcc aataggacg caaattaaa gtcgcaagg aagggaaat gaggtgtgt gtgacgcac caagaggag tgggaagtt atcgaagat gtggatgtg gatgatgag gggtgttat tcgaattgt tggccgcag gattggctg ccgaaggac tgtccaggg gtgagggtc attgctttg aattacacc acggatgtg ctgtggtgt ccagtgtgg aagaaaaag agacgaagg actgacatg gtcgaaagg agcgacgaa atgatcgcc gagctttgc aaattaggg gtcggcaaa caaccgatt atttgggtg ggtcactct aagggcggt ctcttcatt aaacaaatc ataatgaac gcatgggag cggcacagc caatgcccc gaaaaattc gcaattttc caaaacagc aaaggcatc atgttctac agcgtgccg cacaaaggg tcagtctta gcggacttc actttgccg cttcttaga cgaagcgtc gaacttata gaaatacaa aggaactgt gatttcgta ctagacctg cacaagcgg tttctagag ctgtgcaag caggggcat ttaggcgcc gatattttt agttttatc gaaacttcg tttacttta atgtcgttc gtttatttg aagattgtc gcgtacgaa tctgcagac cccgatgtt gggattaaa tgcgacgtg cctctggac cacagagaa atatgcaag cccgcagga cgggactgc ttcctctac ttggagcta atcaagctg attaaaaaa cacagtttg ggtggagaa acaatgtga
- >TC001302 RA
tattacgtg ataccgcag acggagaaa actacatta caacattgt ttccacctg ataatcagt gttgttttt agttgtttt aacaatgga attaaatga ttcaaggga tagcttatc agtggacga ttcgctgct agacgaggt ggaggatat aatgtgcga ccaaacgat tatttccgg agagcttag tgttgtaga tttggagca caagacgcc gtatattat tatatcagc ggcacgcct gcgggtggc cgtggccgt cttcgcccg caccgtaat gcccacagt cctgaaaat ctactacct gtaccaaag aaaccaaca aatcgtggt ggacaacgt ccgccaccg cctccttcc aaccctagt ggccttcct agccaacag cctctcaaa ctccctagc ctcagtcca gaaacgtat tactttccc cctgaaccg tcgtgccat caaagacat ccaaaaaga cctaaaccc agccattga cgacactac gaacacaac ccctgaaga aagcgtcga ctgccaagt cctcttctc ccccaaaac cgaaatcct agccgacgt catcttcat ccacggcct ccacggcgg catcgacaa gacttggaa gcaaggcca gtggcgaca caacggcca caaactcaa agaccagtc gcccgtccg gagccaatc aacggggaa tttatacgt tccaccacg aaaacattc tttgaagcg aaccctcag cgatattta caacgccaa taggacgca aattaaagt cgcaaggaa gggaaatga ggtgtgtgt gacgcacca agaggagtg ggaagttat cgaagatgt ggatgtgga tgatgaggg gtgttattc gaattgttg gccgcagga ttggctgcc gaaggactg tccaggggt gagggtcat tgctttgaa ttacaccac ggatgtgct gtggtgtcc agtgtggaa gaaaaagag acgaaggac tgacatggt cgaaaggag cgacgaaat gatcgccga gctttgcaa attaggggt cggcaaaca accgattat ttgggtggg tcactctaa gggcggtct cttcattaa acaaatcat aatgaacgc atgggagcg gcacagcca atgccccga aaaattcgc aattttcca aaacagcaa aggcatcat gttctacag cgtgccgca caaagggtc agtcttagc ggacttcac tttgccgct tcttagacg aagcgtcga acttataga aatacaaag gaactgtga tttcgtact agacctgca caagcggtt tctagagct gtgcaagca ggggcattt aggcgccga tatttttag ttttatcga aacttcgtt tactttaat gtcgttcgt ttatttgaa gattgtcgc gtacgaatc tgcagaccc cgatgttgg gattaaatg cgacgtgcc tctggacca cagagaaat atgcaagcc cgcaggacg ggactgctt cctctactt ggagctaat caagctgat taaaaaaca cagtttggg tggagaaac aatgtgatg ctagtttta gttagggac tttcaattc tcaggtctt tgttatcat tcctgttgt cagtatttg tcggtacca aagtttagg tag
- >TC001302 RB
tattacgtg ataccgcag acggagaaa actacatta caacattgt ttccacctg ataatcagt gttgttttt agttgtttt aacaatgga attaaatga ttcaaggga tagcttatc agtggacga ttcgctgct agacgaggt ggaggatat aatgtgcga ccaaacgat tatttccgg agagcttag tgttgtaga tttggagca caagacgcc gtatattat tatatcagc ggcttcaga atggaaccg gcaaagtac agcgaaccg ggatgtgat agacttgaa cttttggcg gtttcgtgc aatgatacc aaaggaaat gttcattag acgcctgcg ggtggccgt ggccgtctt cgcccgcac cgtaatgcc cacagtcct gaaaatcta ctacctgta ccaaagaaa ccaacaaat cgtggtgga caacgtccg ccaccgcct ccttccaac cctagtggc cttcctagc caacagcct ctcaaactc cctagcctc agtccagaa acgtattac tttccccct gaaccgtcg tgccatcaa agacatcca aaaagacct aaacccagc cattgacga cactacgaa cacaacccc tgaagaaag cgtcgactg ccaagtcct cttctcccc caaaaccga aatcctagc cgacgtcat cttcatcca cggcctcca cggcggcat cgacaagac ttggaagca aggccagtg gcgacacaa cggccacaa actcaaaga ccagtcgcc cgtccggag ccaatcaac ggggaattt atacgttcc accacgaaa acattcttt gaagcgaac cctcagcga tatttacaa cgccaatag gacgcaaat taaagtcgc aaggaaggg aaatgaggt gtgtgtgac gcaccaaga ggagtggga agttatcga agatgtgga tgtggatga tgaggggtg ttattcgaa ttgttggcc gcaggattg gctgccgaa ggactgtcc aggggtgag ggtcattgc tttgaatta caccacgga tgtgctgtg gtgtccagt gtggaagaa aaagagacg aaggactga catggtcga aaggagcga cgaaatgat cgccgagct ttgcaaatt aggggtcgg caaacaacc gattatttg ggtgggtca ctctaaggg cggtctctt cattaaaca aatcataat gaacgcatg ggagcggca cagccaatg ccccgaaaa attcgcaat tttccaaaa cagcaaagg catcatgtt ctacagcgt gccgcacaa agggtcagt cttagcgga cttcacttt gccgcttct tagacgaag cgtcgaact tatagaaat acaaaggaa ctgtgattt cgtactaga cctgcacaa gcggtttct agagctgtg caagcaggg gcatttagg cgccgatat ttttagttt tatcgaaac ttcgtttac tttaatgtc gttcgttta tttgaagat tgtcgcgta cgaatctgc agaccccga tgttgggat taaatgcga cgtgcctct ggaccacag agaaatatg caagcccgc aggacggga ctgcttcct ctacttgga gctaatcaa gctgattaa aaaacacag tttgggtgg agaaacaat gtgatgcta gttttagtt agggacttt caattctca ggtctttgt tatcattcc tgttgtcag tatttgtcg gtaccaaag tttaggtag