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Gene information
General information
TC001291 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid9: 31871..38474
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001291
    iBeetle screen iB_03166
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001291 RA
      MLEFYPNLN LAPAMLPEH NGLNGPDCN GRTDGRLIK 
      CEKTYEMCE GCGQKIHDR YLMRVADAS WHEHCLTCS 
      ICGVQLAHS CYTRNTKLY CKADYDRIF GVKCSRCGD 
      RLLPHEMVM RAQQHVFHL PCFVCVVCC QPLQKGEQF 
      VLRAGQLFC RQDFEKEMY LMQQASSGD DDMLDENSR 
      PRDGRRGPK RPRTILTSA QRRQFKASF EVSPKPCRK 
      VREALAKET GLSVRVVQV WFQNQRAKM KKIQRKAKQ 
      DDGKSPGDK DKGDKDDKI IKQESPSSD HGHFMGMGN 
      LSDSHFPSS SQPLNPNIP YSPDDAYPA HSGESFCSS 
      DISLDDSTN FDHLDEATS DTLSLQNLE LQSSHSHQH 
      DNVPPSGSI ANPIDKLYL MQNSYFSTE Q
    CDS sequence
    • >TC001291 RA
      atgttggaa ttttacccg aacttaaac cttgctccg 
      gctatgttg ccggaacac aatggctta aatggacca 
      gattgcaac ggtcgaact gatggcaga ctaataaaa 
      tgtgaaaaa acttatgaa atgtgtgag ggttgtggg 
      cagaaaatc cacgatcgg tacctgatg cgggtcgca 
      gacgcaagc tggcacgag cactgcctc acttgtagc 
      atctgtggc gtccaactc gcccattcc tgctacacc 
      agaaacaca aagctctac tgtaaggct gactacgat 
      aggatattt ggagtaaag tgttcaaga tgcggcgac 
      cgtctcctc ccccacgaa atggtaatg agggcccaa 
      cagcacgtc tttcatctg ccctgtttc gtatgcgtg 
      gtgtgttgt cagcctctg caaaaaggc gagcaattc 
      gtgctccgt gcaggccaa ctcttctgt cgacaagac 
      tttgaaaaa gagatgtat ttgatgcag caagcaagc 
      tccggagac gacgacatg ctggacgaa aacagcaga 
      ccaagagac ggaagacga ggccccaaa agacccagg 
      accatccta acctcggcc cagagacgc caatttaaa 
      gcgtcgttc gaagtcagc cccaaaccc tgcagaaaa 
      gtcagggaa gcgctggct aaggaaact ggactcagt 
      gtcagagtt gtgcaagtg tggttccag aaccaaaga 
      gccaagatg aagaaaata caaagaaaa gcgaaacaa 
      gatgatgga aaatcgcca ggagataaa gacaagggg 
      gataaggat gacaagatt atcaaacaa gagtcgcct 
      tctagtgat catggacat tttatgggt atgggaaat 
      ctgagtgat tcacatttt cctagttcc agtcaaccg 
      ctcaacccc aacattccg tactctcca gatgatgct 
      tatccagct cactctggt gaaagtttc tgcagctct 
      gacatctca ttggatgac agtacgaat ttcgatcat 
      ttggatgaa gcaacatca gacaccctt tcattacaa 
      aatttagaa cttcagtcg tcacattca catcaacat 
      gataacgtt ccgccttcc ggttctata gcaaatcca 
      attgataag ctctacctc atgcaaaat tcttatttt 
      agtacggaa caataa
    mRNA sequence
    • >TC001291 RA
      gattacgta aaaaaacca gtgcgtgag tgtcttgtg 
      aggactttt tcaaaatgt tggaatttt acccgaact 
      taaaccttg ctccggcta tgttgccgg aacacaatg 
      gcttaaatg gaccagatt gcaacggtc gaactgatg 
      gcagactaa taaaatgtg aaaaaactt atgaaatgt 
      gtgagggtt gtgggcaga aaatccacg atcggtacc 
      tgatgcggg tcgcagacg caagctggc acgagcact 
      gcctcactt gtagcatct gtggcgtcc aactcgccc 
      attcctgct acaccagaa acacaaagc tctactgta 
      aggctgact acgatagga tatttggag taaagtgtt 
      caagatgcg gcgaccgtc tcctccccc acgaaatgg 
      taatgaggg cccaacagc acgtctttc atctgccct 
      gtttcgtat gcgtggtgt gttgtcagc ctctgcaaa 
      aaggcgagc aattcgtgc tccgtgcag gccaactct 
      tctgtcgac aagactttg aaaaagaga tgtatttga 
      tgcagcaag caagctccg gagacgacg acatgctgg 
      acgaaaaca gcagaccaa gagacggaa gacgaggcc 
      ccaaaagac ccaggacca tcctaacct cggcccaga 
      gacgccaat ttaaagcgt cgttcgaag tcagcccca 
      aaccctgca gaaaagtca gggaagcgc tggctaagg 
      aaactggac tcagtgtca gagttgtgc aagtgtggt 
      tccagaacc aaagagcca agatgaaga aaatacaaa 
      gaaaagcga aacaagatg atggaaaat cgccaggag 
      ataaagaca agggggata aggatgaca agattatca 
      aacaagagt cgccttcta gtgatcatg gacatttta 
      tgggtatgg gaaatctga gtgattcac attttccta 
      gttccagtc aaccgctca accccaaca ttccgtact 
      ctccagatg atgcttatc cagctcact ctggtgaaa 
      gtttctgca gctctgaca tctcattgg atgacagta 
      cgaatttcg atcatttgg atgaagcaa catcagaca 
      ccctttcat tacaaaatt tagaacttc agtcgtcac 
      attcacatc aacatgata acgttccgc cttccggtt 
      ctatagcaa atccaattg ataagctct acctcatgc 
      aaaattctt attttagta cggaacaat aaaaatgtt 
      ttaattttt ttgttataa attatcaca tatttaatt 
      ttgtattat ttttgttgt tttgttaag taaaaccaa 
      attccttta tttgttgct atgcaagtg ccatttgag 
      ctcaataaa actagtcac agagt