Status
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Gene information
General information
TC001252 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13231151..13235667
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001252
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001252 RA
      MNLMFRKNF REQKSGVAS KTSKACANR HASKRASRE 
      QGYTPMEEH HYRTHLFFN PSRCSYAQA AEDMDEPRC 
      GPMTGAGGE PLPPLQSSA ISPPMTESD LPQPLVVRT 
      PSVSMMKWP TTPSPTKTT PTRQVSFNY GNGNTETAR 
      TLSGLERDC FIIPVHSLD RFLPAGVPM PKPPANGNS 
      LDRKSQGNL HVMEVPDPK LCVLCHLMS PLEPIDPVL 
      ESPLVQPLF KQRVTAGEL LSEISQSKT AVTGMLLAN 
      MERNAEFPF ISYYVINTS QTNPVMFYN NIRTASLTK 
      FDPRQLRYT AAHTLDLYT EVASICRPP VHEIGVSLT 
      KTHTSTTGY MVSVYKVFE GDDREKFER NWLYWTGAR 
      MIYRYLPAA AGLRRISLH KSLSPKGDK MYILICECC 
      NLLSDVTVC ALILPALRA RLTGYTGIF RPIQTF
    • >TC001252 RB
      MNPFAEPRC GPMTGAGGE PLPPLQSSA ISPPMTESD 
      LPQPLVVRT PSVSMMKWP TTPSPTKTT PTRQVSFNY 
      GNGNTETAR TLSGLERDC FIIPVHSLD RFLPAGVPM 
      PKPPANGNS LDRKSQGNL HVMEVPDPK LCVLCHLMS 
      PLEPIDPVL ESPLVQPLF KQRVTAGEL LSEISQSKT 
      AVTGMLLAN MERNAEFPF ISYYVINTS QTNPVMFYN 
      NIRTASLTK FDPRQLRYT AAHTLDLYT EVASICRPP 
      VHEIGVSLT KTHTSTTGY MVSVYKVFE GDDREKFER 
      NWLYWTGAR MIYRYLPAA AGLRRISLH KSLSPKGDK 
      MYILICECC NLLSDVTVC ALILPALRA RLTGYTGIF 
      RPIQTF
    • >TC001252 RC
      MNLMFRKNF REQKSGVAS KTSKACANR HASKRASRE 
      QGYTPMEEH HYRTHLFFN PSRCSYAQA AEDMDGTAT 
      GNKVKEKAV CFYQRSDRA ALPVL
    CDS sequence
    • >TC001252 RA
      atgaatctg atgttccgg aaaaacttc cgggagcaa 
      aaaagcggc gtcgcgagc aaaacttcg aaagcttgt 
      gccaacaga catgcgagc aagagggcc agtcgggag 
      caggggtac acccccatg gaggagcac cactatcgg 
      acacatctg ttcttcaac ccgtcgaga tgtagttac 
      gctcaggcg gctgaagac atggacgaa ccgcgttgc 
      ggtcctatg actggagcg ggcggggaa cccttgccg 
      cctctgcag tcgtctgcg atatctcct ccgatgacg 
      gagtccgac ctgccccag cctttggtc gttcgaact 
      ccttctgtc tcgatgatg aagtggcct acgacgccg 
      tcgcccacc aaaaccacc cccaccaga caagttagt 
      ttcaactac gggaatggg aatacggag actgcgagg 
      accttgtct ggtcttgaa agggactgc tttatcatc 
      ccggttcac agtttagat aggtttctt ccagcagga 
      gtaccaatg ccaaaaccc ccggctaac gggaattct 
      ttggacaga aagtcccaa gggaactta catgtgatg 
      gaggtgcct gatccaaaa ctgtgtgtc ttatgccat 
      ttaatgagt cccttggag cccatcgac cccgttttg 
      gagtcaccc ttggtccag cccttattc aagcaaaga 
      gtaacagcc ggggaacta cttagcgaa atttcccaa 
      tcgaaaacg gcggtcact gggatgcta ttagccaac 
      atggaacgc aacgccgaa ttccctttc atatcatat 
      tatgtgata aacacctca caaacaaat cctgtgatg 
      ttttacaat aatattcgc actgccagc ctgaccaag 
      ttcgaccct cgacaactt aggtataca gcggcgcat 
      actctagat ctttacaca gaagtggca tcgatttgt 
      cgacctccg gtccacgaa atcggggtt tcactcaca 
      aaaacgcac acttcgacg acgggatac atggtttcg 
      gtgtacaaa gtgtttgag ggagatgac agggagaag 
      tttgagcgg aactggttg tactggact ggggcccgg 
      atgatctat aggtatctc ccggcggcg gccggactg 
      aggagaatc tccttgcac aagtcgctc agtcccaaa 
      ggggacaag atgtacatt ttgatttgt gaatgctgt 
      aatctactg agcgacgtc acggtttgt gcgctcatt 
      ttaccagcg ttgagggcg cgactgaca gggtacacc 
      ggaattttt agaccaata caaacattc taa
    • >TC001252 RB
      atgaatccg tttgcagaa ccgcgttgc ggtcctatg 
      actggagcg ggcggggaa cccttgccg cctctgcag 
      tcgtctgcg atatctcct ccgatgacg gagtccgac 
      ctgccccag cctttggtc gttcgaact ccttctgtc 
      tcgatgatg aagtggcct acgacgccg tcgcccacc 
      aaaaccacc cccaccaga caagttagt ttcaactac 
      gggaatggg aatacggag actgcgagg accttgtct 
      ggtcttgaa agggactgc tttatcatc ccggttcac 
      agtttagat aggtttctt ccagcagga gtaccaatg 
      ccaaaaccc ccggctaac gggaattct ttggacaga 
      aagtcccaa gggaactta catgtgatg gaggtgcct 
      gatccaaaa ctgtgtgtc ttatgccat ttaatgagt 
      cccttggag cccatcgac cccgttttg gagtcaccc 
      ttggtccag cccttattc aagcaaaga gtaacagcc 
      ggggaacta cttagcgaa atttcccaa tcgaaaacg 
      gcggtcact gggatgcta ttagccaac atggaacgc 
      aacgccgaa ttccctttc atatcatat tatgtgata 
      aacacctca caaacaaat cctgtgatg ttttacaat 
      aatattcgc actgccagc ctgaccaag ttcgaccct 
      cgacaactt aggtataca gcggcgcat actctagat 
      ctttacaca gaagtggca tcgatttgt cgacctccg 
      gtccacgaa atcggggtt tcactcaca aaaacgcac 
      acttcgacg acgggatac atggtttcg gtgtacaaa 
      gtgtttgag ggagatgac agggagaag tttgagcgg 
      aactggttg tactggact ggggcccgg atgatctat 
      aggtatctc ccggcggcg gccggactg aggagaatc 
      tccttgcac aagtcgctc agtcccaaa ggggacaag 
      atgtacatt ttgatttgt gaatgctgt aatctactg 
      agcgacgtc acggtttgt gcgctcatt ttaccagcg 
      ttgagggcg cgactgaca gggtacacc ggaattttt 
      agaccaata caaacattc taa
    • >TC001252 RC
      atgaatctg atgttccgg aaaaacttc cgggagcaa 
      aaaagcggc gtcgcgagc aaaacttcg aaagcttgt 
      gccaacaga catgcgagc aagagggcc agtcgggag 
      caggggtac acccccatg gaggagcac cactatcgg 
      acacatctg ttcttcaac ccgtcgaga tgtagttac 
      gctcaggcg gctgaagac atggacggg accgccaca 
      ggtaataaa gtcaaggaa aaagcagtt tgtttctat 
      caaaggtca gatcgggct gctttgcca gttttgtaa
    mRNA sequence
    • >TC001252 RA
      ttagcagta ggtcatcag gtgcgccac cactgacct 
      accgcgtgt gagtaataa ttccgcgca ccttttata 
      aatagatgg attcgtccg agttgtttc gccggattc 
      aagtcttca gaagtagcg tcgttagcg tcgcgacgc 
      cgtgaccgt ggatgctac atctctaaa aatagtgtc 
      aggtgtcgg caattacgt tctttacct taatgaggc 
      cttatttac gaattgccc gaatcaatt tgccaagac 
      tgttgtaag aaccaccgt caccatgaa tctgatgtt 
      ccggaaaaa cttccggga gcaaaaaag cggcgtcgc 
      gagcaaaac ttcgaaagc ttgtgccaa cagacatgc 
      gagcaagag ggccagtcg ggagcaggg gtacacccc 
      catggagga gcaccacta tcggacaca tctgttctt 
      caacccgtc gagatgtag ttacgctca ggcggctga 
      agacatgga cgaaccgcg ttgcggtcc tatgactgg 
      agcgggcgg ggaaccctt gccgcctct gcagtcgtc 
      tgcgatatc tcctccgat gacggagtc cgacctgcc 
      ccagccttt ggtcgttcg aactccttc tgtctcgat 
      gatgaagtg gcctacgac gccgtcgcc caccaaaac 
      cacccccac cagacaagt tagtttcaa ctacgggaa 
      tgggaatac ggagactgc gaggacctt gtctggtct 
      tgaaaggga ctgctttat catcccggt tcacagttt 
      agataggtt tcttccagc aggagtacc aatgccaaa 
      acccccggc taacgggaa ttctttgga cagaaagtc 
      ccaagggaa cttacatgt gatggaggt gcctgatcc 
      aaaactgtg tgtcttatg ccatttaat gagtccctt 
      ggagcccat cgaccccgt tttggagtc acccttggt 
      ccagccctt attcaagca aagagtaac agccgggga 
      actacttag cgaaatttc ccaatcgaa aacggcggt 
      cactgggat gctattagc caacatgga acgcaacgc 
      cgaattccc tttcatatc atattatgt gataaacac 
      ctcacaaac aaatcctgt gatgtttta caataatat 
      tcgcactgc cagcctgac caagttcga ccctcgaca 
      acttaggta tacagcggc gcatactct agatcttta 
      cacagaagt ggcatcgat ttgtcgacc tccggtcca 
      cgaaatcgg ggtttcact cacaaaaac gcacacttc 
      gacgacggg atacatggt ttcggtgta caaagtgtt 
      tgagggaga tgacaggga gaagtttga gcggaactg 
      gttgtactg gactggggc ccggatgat ctataggta 
      tctcccggc ggcggccgg actgaggag aatctcctt 
      gcacaagtc gctcagtcc caaagggga caagatgta 
      cattttgat ttgtgaatg ctgtaatct actgagcga 
      cgtcacggt ttgtgcgct cattttacc agcgttgag 
      ggcgcgact gacagggta caccggaat ttttagacc 
      aatacaaac attctaaga gaatttcag acatatcgc 
      aggcactgg gaattgctg gggacttcc tttgttcaa 
      attttgttt ttgggcgac aaacatttc attaccgtc 
      ctgtcttgg taattcaag cgacaatac ttatagtca 
      aaaaaagtt ttgacgagc aattttcac tcctagagt 
      acaagaaaa caacagttc tctaataaa ttgaaaagt 
      attgaaaga ttgtggact tagattcct gtagacgaa 
      aacaattta attgtgact tttgaaatg ttttgagaa 
      attattcac atacaagtg ccccaagtt tgatcaaaa 
      actatcgag agaagctcg aggaacatt tttccaaat 
      tttcataga agttttata atgaatatt agaccgcaa 
      gtttgaact aagcttctt gcaaacgtg cctatttac 
      attttatta taataagaa caatcaaat atttatcaa 
      attgtttag tattagtta tagacattc cctcaaata 
      ataccttgg gcaagctat aaccgtaag ttttttttt 
      caaacgtca ctgttgaaa catatcttg ttgaaacaa 
      aaagtgata ataacaata taaattagt acaaaattt 
      acggctttt tgttacttt atgtaatgt ttaaatgta 
      tgatgaaaa ttattgttg cggcatttg ccgccagtg 
      ttatccacc tatggctgt atttttatc aaaacctca 
      catcaataa tgtaaacac catgattgt aaaatttac 
      gacctttag gcaacaagt cttccttag tttcgcttt 
      caacaacag caatataag agagagatt tatttaaat 
      atacgcaat gctcaaga
    • >TC001252 RB
      ttagcagta ggtcatcag gtgcgccac cactgacct 
      accgcgtgt gagtaataa ttccgcgca ccttttata 
      aatagatgg attcgtccg agttgtttc gccggattc 
      aagtcttca gaagtagcg tcgttagcg tcgcgacgc 
      cgtgaccgt ggatgctac atctctaaa aatagtgtc 
      aggtgtcgg caattacgt tctttacct taatgaggc 
      cttatttac gaattgccc gaatcaatt tgccaagac 
      tgttgtaag aaccaccgt caccatgaa tctgatgtt 
      ccggaaaaa cttccggga gcaaaaaag cggcgtcgc 
      gagcaaaac ttcgaaagc ttgtgccaa cagacatgc 
      gagcaagag ggccagtcg ggagcaggg gtacacccc 
      catggagga gcaccacta tcggacaca tctgttctt 
      caacccgtc gagatgtag ttacgctca ggcggctga 
      agacatgga cggtacgct ttattcatg cttttcccc 
      tgaaaatac cgccctcag tgcacaaac aataccttt 
      caattgtct ttcacaggg accgccaca ggtaataaa 
      gtcaaggaa aaagcagtt tgtttctat caaaggtca 
      gatcgggct gctttgcca gttttgtaa agagattaa 
      tcattaaat tagcttggt aaagggcgc actaggcgc 
      ttgtactta gaaataaac agcttagga ccgtaataa 
      cagcatcag tgtttaaag cttccctct gtatatatg 
      tccagttac gttctcgaa actcgaaaa aaaatattt 
      tgaaaaact ttttggcga aaaaaggca aattctgcc 
      aaataaata ttttttaat tgaaaattt taaatttct 
      ttgttctat tcggaaccc gaaaaaaat atatctgag 
      cttgtattc ccgtgggtg gattttcta tttataaaa 
      agctaaata aagcgaaca atcgtatga atgtttaca 
      aaatttccg ttgcaattg gcatttgcg gaagtctaa 
      tttttcagc gaccaaaaa tgttggtcc ttgaacgcc 
      tgaaagagt gttggcagc agttgcagg taaattgaa 
      tcagtagcc tctctattt tctgcaata gacgagctt 
      cgtataagt attgacacg cgtaaaagt tggccagtg 
      ttgctcaaa ggtccattt ttaggcgca ataatcgaa 
      aaaaagtaa gttactgtt aaatcccaa ctgaaacaa 
      tgctttgtc gggtggacg aacttgtcg gtatttcga 
      caaaaacaa aaatacatt tcttactaa agtgaacgt 
      gagtataaa aatacctcg tcttgccaa aacattgtg 
      gcaaaattc tcgaaccgt tcgacgttg tgcaagaaa 
      tcgtcgctc tcgctcgcg cacattttg accactcga 
      caggtggcc taacaatta gtgtcataa cacgggacg 
      tattttttg ggctgaata atttagttt gggttcacc 
      gtttttgca cgtccgtcg acacaacta acccttttt 
      gattaaaca gtaaattag taatgtccc tttttcgag 
      agagatgca tgcgcgcac ttcaccact ggcctatta 
      ttggtccat ttgtttgat tttctttcg tatgaatcc 
      gtttgcaga accgcgttg cggtcctat gactggagc 
      gggcgggga acccttgcc gcctctgca gtcgtctgc 
      gatatctcc tccgatgac ggagtccga cctgcccca 
      gcctttggt cgttcgaac tccttctgt ctcgatgat 
      gaagtggcc tacgacgcc gtcgcccac caaaaccac 
      ccccaccag acaagttag tttcaacta cgggaatgg 
      gaatacgga gactgcgag gaccttgtc tggtcttga 
      aagggactg ctttatcat cccggttca cagtttaga 
      taggtttct tccagcagg agtaccaat gccaaaacc 
      cccggctaa cgggaattc tttggacag aaagtccca 
      agggaactt acatgtgat ggaggtgcc tgatccaaa 
      actgtgtgt cttatgcca tttaatgag tcccttgga 
      gcccatcga ccccgtttt ggagtcacc cttggtcca 
      gcccttatt caagcaaag agtaacagc cggggaact 
      acttagcga aatttccca atcgaaaac ggcggtcac 
      tgggatgct attagccaa catggaacg caacgccga 
      attcccttt catatcata ttatgtgat aaacacctc 
      acaaacaaa tcctgtgat gttttacaa taatattcg 
      cactgccag cctgaccaa gttcgaccc tcgacaact 
      taggtatac agcggcgca tactctaga tctttacac 
      agaagtggc atcgatttg tcgacctcc ggtccacga 
      aatcggggt ttcactcac aaaaacgca cacttcgac 
      gacgggata catggtttc ggtgtacaa agtgtttga 
      gggagatga cagggagaa gtttgagcg gaactggtt 
      gtactggac tggggcccg gatgatcta taggtatct 
      cccggcggc ggccggact gaggagaat ctccttgca 
      caagtcgct cagtcccaa aggggacaa gatgtacat 
      tttgatttg tgaatgctg taatctact gagcgacgt 
      cacggtttg tgcgctcat tttaccagc gttgagggc 
      gcgactgac agggtacac cggaatttt tagaccaat 
      acaaacatt ctaagagaa tttcagaca tatcgcagg 
      cactgggaa ttgctgggg acttccttt gttcaaatt 
      ttgtttttg ggcgacaaa catttcatt accgtcctg 
      tcttggtaa ttcaagcga caatactta tagtcaaaa 
      aaagttttg acgagcaat tttcactcc tagagtaca 
      agaaaacaa cagttctct aataaattg aaaagtatt 
      gaaagattg tggacttag attcctgta gacgaaaac 
      aatttaatt gtgactttt gaaatgttt tgagaaatt 
      attcacata caagtgccc caagtttga tcaaaaact 
      atcgagaga agctcgagg aacattttt ccaaatttt 
      catagaagt tttataatg aatattaga ccgcaagtt 
      tgaactaag cttcttgca aacgtgcct atttacatt 
      ttattataa taagaacaa tcaaatatt tatcaaatt 
      gtttagtat tagttatag acattccct caaataata 
      ccttgggca agctataac cgtaagttt ttttttcaa 
      acgtcactg ttgaaacat atcttgttg aaacaaaaa 
      gtgataata acaatataa attagtaca aaatttacg 
      gctttttgt tactttatg taatgttta aatgtatga 
      tgaaaatta ttgttgcgg catttgccg ccagtgtta 
      tccacctat ggctgtatt tttatcaaa acctcacat 
      caataatgt aaacaccat gattgtaaa atttacgac 
      ctttaggca acaagtctt ccttagttt cgctttcaa 
      caacagcaa tataagaga gagatttat ttaaatata 
      cgcaatgct caaga
    • >TC001252 RC
      ttagcagta ggtcatcag gtgcgccac cactgacct 
      accgcgtgt gagtaataa ttccgcgca ccttttata 
      aatagatgg attcgtccg agttgtttc gccggattc 
      aagtcttca gaagtagcg tcgttagcg tcgcgacgc 
      cgtgaccgt ggatgctac atctctaaa aatagtgtc 
      aggtgtcgg caattacgt tctttacct taatgaggc 
      cttatttac gaattgccc gaatcaatt tgccaagac 
      tgttgtaag aaccaccgt caccatgaa tctgatgtt 
      ccggaaaaa cttccggga gcaaaaaag cggcgtcgc 
      gagcaaaac ttcgaaagc ttgtgccaa cagacatgc 
      gagcaagag ggccagtcg ggagcaggg gtacacccc 
      catggagga gcaccacta tcggacaca tctgttctt 
      caacccgtc gagatgtag ttacgctca ggcggctga 
      agacatgga cgggaccgc cacaggtaa taaagtcaa 
      ggaaaaagc agtttgttt ctatcaaag gtcagatcg 
      ggctgcttt gccagtttt gtaaagaga ttaatcatt 
      aaattagct tggtaaagg gcgcactag gcgcttgta 
      cttagaaat aaacagctt aggaccgta ataacagca 
      tcagtgttt aaagcttcc ctctgtata tatgtccag 
      ttacgttct cgaaactcg aaaaaaaat attttgaaa 
      aactttttg gcgaaaaaa ggcaaattc tgccaaata 
      aatattttt taattgaaa attttaaat ttctttgtt 
      ctattcgga acccgaaaa aaatatatc tgagcttgt 
      attcccgtg ggtggattt tctatttat aaaaagcta 
      aataaagcg aacaatcgt atgaatgtt tacaaaatt 
      tccgttgca attggcatt tgcggaagt ctaattttt 
      cagcgacca aaaatgttg gtccttgaa cgcctgaaa 
      gagtgttgg cagcagttg caggtaaat tgaatcagt 
      agcctctct attttctgc aatagacga gcttcgtat 
      aagtattga cacgcgtaa aagttggcc agtgttgct 
      caaaggtcc atttttagg cgcaataat cgaaaaaaa 
      gtaagttac tgttaaatc ccaactgaa acaatgctt 
      tgtcgggtg gacgaactt gtcggtatt tcgacaaaa 
      acaaaaata catttctta ctaaagtga acgtgagta 
      taaaaatac ctcgtcttg ccaaaacat tgtggcaaa 
      attctcgaa ccgttcgac gttgtgcaa gaaatcgtc 
      gctctcgct cgcgcacat tttgaccac tcgacaggt 
      ggcctaaca attagtgtc ataacacgg gacgtattt 
      tttgggctg aataattta gtttgggtt caccgtttt 
      tgcacgtcc gtcgacaca actaaccct ttttgatta 
      aacagtaaa ttagtaa
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