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Gene information
General information
TC001242 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7637619..7644103
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001242
    iBeetle screen iB_03162
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001242 RA
      MDRNKPGQS QATRGPKAK ITQQPPDLF IALGAKQSS 
      PRDDPKRSV PLPKTSTAS HLQTSSSKV TDKLKREGT 
      SSSLTVPPS KKMKVAIPG SRPGPSTSS ILPVERMGV 
      EPWEALAVD CETSDLFES IMAAVNAGN TDKAVGYVL 
      GVIRSLKTQ RTKICKVVY LSLLLTCSC KPQLFTNEN 
      IIAAIVNVL RRDVTSGFK GVNKTNSYF HMLFINLLT 
      HAFTDVAQW PEIFVKIYV EDAVADRIF IDNPYCKPF 
      VDNIITAFN TKLPPSSLL KSETWNSTA RDTSSPLTI 
      TSADDDTKS EVILEHKAA VENWNVKVY PRFTQTQEN 
      VEKIVLEAI KEQLARRQQ PETITKNFV RFLSIASGL 
      VEIRIIAVS RIESWLHNH KLMKSAQEL LAYLCYNCS 
      ASSQRDLEI IAQLSKLRL KNKPMVNYF NNCLREMVY 
      SFPENLYPL LKYTIYNEL SNARNSNNL IVVGALFQV 
      APEASADAF ADICLELLL NKDDYLRSL RALLKEITR 
      VLRHDFNLL SIVHSLVRE RKEFANNVR ESEFRERIF 
      LSLADLVCM CMLLCVSPQ VRDASTQNK RDVTVLKAF 
      QKQVSNIQR EAVTWLNDS ALRVFRPSL NDFHHVLLK 
      VLFLEQAEH YYKVDSWPG ENERNLFLR LTSEVPLLQ 
      ATLLRILLI GNSKEHPIS PTEAIEISD QLIRRAANL 
      PQDCAPPLV VDNLEIIGI YFNLCSYNY PENITLPAG 
      YVPPSLAIS GLYWKVWLI LLIIAAHNP VTFGNEVWN 
      KYPTLRMFM EMCITNHFS FPPPTMTSL DEDYQSREQ 
      QILALEKQK ILEFESHLA AASTKMEIT EQSSLLLPQ 
      LMELRPEGE ARRPPQAVL DQLQHLNNT HRMGHLLCR 
      SRNPDFLLD IMSRQGGTA HMPWLAELV HNSEGALAH 
      LPVQCLCEY LLSTTPTEK LTKHGQLLA HLRTVVNEN 
      DPQTACEVL EYLFRRLTS DHGASRTQA TKGLNLILA 
      EGEESETNN TNWLTSYIT HFPHFTMIK PVLIQFLRQ 
      ALQIETNPA RVSSYINFL ATQDCNDSF TELHELIAD 
      LSSVIIERH SVASYVLPG PDNQTLKHL LTIFASHIQ 
      KAQEFSEDI YSSVQNYSE YVLVTWVTG EQCHMQTLV 
      IHAAIVLLT YGPLENFEP FETLLNFWF PANAEQPQA 
      YTPDTNERT SYLPDWMKL RMIRSNVPR LVDAAIEKL 
      EPPKLVLFI QSFGIPTNS ISKLLSTLD KATLLDPKL 
      VVDSVLDKT YMIQLVEVQ NKRGAVGGD TFVKAIEMQ 
      MPVVQDIDV KIEIESKKQ LPNLVKTQQ IVTNSGDII 
      NLLNNIFSP NYGGSKDRD IVTVTKMAF EKQSLDVIF 
      NYLKSLPMN FIAPVLHKH NSISTLFRL LFSSKDHLI 
      DKITMATVF LNHMTDVHN ITYSVLRQF LNENDQKVK 
      TEEQGERKL VSMISEAKT QEEKTDMMV DWLSTIQLE 
      VAYSQKQLK EDLLFSRDN LPFRPLLMS LVLQRASWQ 
      SLHVIMNYL LKEDNSDIC PVSALDFLC ALTQSPKLW 
      QGRDKAIPK HHHSEDVFD LGKDKALIL VGYILEESK 
      QNQANWSKK MESRLVLLL QCIRNCAPE ILGTLLEKS 
      DCDTHCREL LLMIYMSLP YSGQDLINS KDNISKQAY 
      AKNCTSSAD EISHCLLSA LAATPRSKD WPRKSQDLE 
      LCARKLAAT HPVLVLRQL PMLAGSLKG RAQYDWGVL 
      KNRGHLLLF GQVLGLLEL LEPIVFEQS RTLCDILDS 
      YFLLLQYHG HMKDLSVLV QRIITFIQN WMTNDIKAA 
      SRYLQEHGS VLLDIQMIQ PGVRPLLSN VTLPVGDSS 
      TPPELLVRT AMSPMMDPL PPHWSQLLT AIQTSENLN 
      ILQELDHIT NKKPHLLET PSQHLYNYI SSSSGAVRS 
      LALTLIVRW LKFNPNAAS DALPVILTC LDSRNGDVV 
      ASVLDRLSD LVSVMQEYG KVILTRVFQ LGMESTLNT 
      TSNITKSID LLNLQSGC
    CDS sequence
    • >TC001242 RA
      atggatcgg aataaaccg ggccagagc caagccact 
      cgtggcccc aaggccaaa ataacgcaa caaccccct 
      gacttgttc atcgctttg ggggccaaa caatcctcc 
      cctcgagat gaccccaaa cgtagcgtt ccgctccct 
      aaaaccagc actgcctcc catttacaa acaagtagc 
      agtaaagtg acggataaa ttgaagcgt gaagggact 
      tcgtctagt cttacagtg cccccttcg aagaaaatg 
      aaagttgcg ataccggga agccggcca gggccctcc 
      actagcagc atactacct gtggagagg atgggggtt 
      gagccgtgg gaagccctg gctgtggac tgtgagaca 
      agcgatttg ttcgagagt attatggcg gctgttaat 
      gccggcaat actgataaa gctgttggg tatgttttg 
      ggggtcatt cgctcgttg aagactcaa aggactaag 
      atttgtaaa gtggtttat ctgagtctt ttactgact 
      tgcagctgc aaaccccag ttattcact aatgagaat 
      atcatagcg gctattgtg aatgttctg cggcgtgat 
      gtaacttcc ggttttaag ggcgttaac aaaacaaat 
      tcttacttc cacatgctg tttattaat ttattaact 
      cacgccttc accgatgtc gcccaatgg ccggaaatt 
      ttcgtcaaa atttatgtg gaggacgcg gtggccgat 
      cgcattttt atcgacaat ccgtactgt aaaccattc 
      gttgataac ataatcacg gcgtttaac accaaattg 
      cccccttcc tcgctcctc aaatccgag acatggaat 
      tccacggct agagacact tctagcccc ctcacaatc 
      acaagcgct gacgacgac accaaaagc gaagtgatt 
      ctcgagcat aaagccgca gttgaaaac tggaacgtc 
      aaagtgtat ccccggttc acccaaacg caggaaaac 
      gtcgaaaaa atcgtcctt gaagccatc aaggaacaa 
      ctagcaagg cgccaacaa ccggaaacc atcacgaaa 
      aacttcgtc cgcttcttg agtatcgct tcaggactt 
      gtggaaatt cgcataatt gccgtttcg cgcatcgag 
      agttggcta cacaaccat aagttgatg aaatcagcg 
      caggaactc ctcgcctat ttgtgctac aattgctcg 
      gcgagttcg caaagagac ttggaaata atagcgcag 
      ctgagcaag ctcagactg aagaacaaa cccatggtt 
      aactacttc aacaactgt ctccgggag atggtttac 
      tcgttcccg gagaacttg taccccctt ttgaaatac 
      acgatttac aacgaactg tccaatgcc cgaaattcc 
      aacaattta atcgtcgtg ggggccctg tttcaggtc 
      gcacctgag gcaagtgcc gacgctttc gccgacatt 
      tgcctggag ttgctccta aacaaagac gactatttg 
      cgttcgctg cgggcgctt ttgaaagaa atcacccga 
      gtcctccgg cacgatttc aacttgtta agcatcgtg 
      cattcgctc gttcgcgag cgcaaagag tttgcgaat 
      aacgtgcgc gagtcggaa tttcgcgag cgaattttt 
      ctatcactg gccgacctg gtctgcatg tgtatgctg 
      ctgtgcgtc agccctcag gtccgcgac gcctcaacg 
      caaaacaag cgcgacgtc acggtcctg aaagccttc 
      cagaaacaa gtcagtaac atacagcgg gaggcagtg 
      acttggctc aacgactcc gcgctaagg gttttccgg 
      ccaagtctc aacgacttc caccacgtt ctactcaaa 
      gtcttgttc ctcgaacaa gcggagcat tactacaag 
      gtcgactcg tggcctggc gaaaacgag aggaacttg 
      tttttgagg cttacgagt gaggttccc ttgcttcag 
      gcgactctt ttgaggatt ttgctgatc gggaactcg 
      aaagaacac ccgattagt cccacggaa gcgattgaa 
      attagcgat cagttgatt agaagggcg gcgaatttg 
      ccccaagac tgcgccccg cccttggtc gttgataat 
      ttggagata atcgggatt tatttcaac ttgtgcagc 
      tataattat ccggagaat atcacgctg ccggcgggg 
      tatgtgccc cccagtttg gccatttct gggctctat 
      tggaaagtt tggctcatt ttgctcata atagcggct 
      cataatccg gtcactttt gggaacgag gtctggaac 
      aagtaccca acgttgcgc atgttcatg gaaatgtgc 
      attacaaat cacttttcg tttcccccg cccaccatg 
      acctccctg gacgaagac taccagtcc cgcgagcag 
      caaatcctc gcccttgag aagcaaaaa atcctggaa 
      tttgagtca cacctcgcg gccgcgagc actaaaatg 
      gaaatcacc gaacagagc agcctccta ttgccccaa 
      ttaatggaa ctgcggcct gagggcgaa gcccgaagg 
      cccccccaa gccgtcctc gaccaactc caacacttg 
      aacaacacg catcgcatg ggacattta ttgtgccgc 
      tcgcggaac cccgatttt ctattggac attatgtcg 
      cggcaaggg ggcaccgcg catatgccg tggctggcc 
      gagctggtc cacaattcg gagggggcg ttggcccac 
      ctgcctgtg caatgtctg tgcgagtat ctcctgtcg 
      acgacaccg acggagaaa ttgacgaaa catggccag 
      cttttggcg catttgcgg acggtggtt aatgagaat 
      gatccgcag acggcctgt gaggttttg gagtatttg 
      ttcaggcgg ttgacgtcc gatcatggg gcgagtcgg 
      acgcaggcc accaaaggc ttgaatttg attttggcc 
      gaaggggag gaaagcgag acgaataat acgaactgg 
      ttgacgagt tacataaca cattttcca cactttacg 
      atgattaaa ccagtgctt atacagttt ttgcggcaa 
      gccttgcaa attgagaca aatccggcg cgggtttcg 
      agttatatc aattttttg gcaacgcag gattgtaac 
      gattcgttt acggaactg cacgaatta attgccgat 
      ttatcgtcg gttattatc gagagacac agcgtggct 
      tcgtacgtg ttgccaggg ccggacaac caaactttg 
      aagcacctt ttgaccatt tttgcgagt catattcag 
      aaagcgcag gaattctcg gaggatatt tacagttcg 
      gtgcaaaac tacagcgag tatgttttg gtgacttgg 
      gtgactggg gaacaatgc cacatgcag actttagta 
      atacacgca gctattgtg ttactgact tacggtcct 
      ttggagaat tttgagccg ttcgagact ttgttaaat 
      ttttggttt ccggcgaac gccgaacaa ccacaagcc 
      tacacacct gatacaaac gaacgaacg tcgtatctc 
      cctgattgg atgaaactg cggatgatt cggtcaaac 
      gtcccacga ttggttgac gcagccatt gaaaaatta 
      gagccgcca aagttggtc ctttttata caatcgttc 
      ggaataccg acaaattcc atcagtaaa ttgctgagt 
      actttggac aaagccaca cttttggac ccgaaactg 
      gtcgtggac tcagttttg gacaaaact tacatgatt 
      caattggtg gaagtccaa aacaaaaga ggcgcagtg 
      ggtggtgac acctttgtc aaggctatc gagatgcaa 
      atgcccgtt gtacaagac attgatgtt aaaatcgaa 
      atcgagagt aaaaaacaa ttacccaac ttagtaaaa 
      acccaacag attgtcaca aatagcggc gatattatc 
      aatttgttg aataatatt tttagccca aactatggc 
      ggttcgaaa gatcgggac attgtcact gtgaccaag 
      atggcgttt gagaaacaa tcactggac gttattttc 
      aattatttg aaatcacta ccgatgaat ttcatagca 
      cctgtgctc cataaacac aattcgatt tcaacgctt 
      ttccgcctg ttattttcg tcaaaagac cacctcatt 
      gacaaaata accatggct acggttttc ctcaaccac 
      atgacagac gttcacaat attacttat tcggttttg 
      aggcaattc ttaaacgaa aacgaccaa aaagtcaaa 
      actgaagaa caaggcgaa cggaaattg gtaagtatg 
      atatcggag gcgaaaaca caggaagaa aaaactgac 
      atgatggtt gactggtta tcaacaatc caactggaa 
      gtggcttac tcgcaaaaa caattgaaa gaagatttg 
      ttgttttcg cgcgacaac ttgccgttt aggccgctt 
      cttatgtcg ctggttttg caaagggcg agttggcag 
      agcctccac gtgataatg aattatttg ctaaaagag 
      gacaattcg gacatttgc cctgtttcc gcccttgat 
      ttcctctgt gccctcacc cagagcccg aagctgtgg 
      caggggcgc gataaagcc attcccaaa caccaccat 
      tcggaggac gtttttgat ttgggcaaa gacaaggcc 
      ctgatcctt gtcggttat attttggag gaaagcaag 
      caaaaccag gcaaactgg tcgaagaaa atggaatcg 
      cggctcgtc ctcttactg cagtgtatc cgaaactgc 
      gccccggag attttgggg acacttttg gagaagtcc 
      gactgtgat acgcactgt cgggagctt ctcttgatg 
      atttacatg tcgctgccg tattccggt caagatttg 
      atcaatagc aaagataac atttcgaag caggcgtat 
      gcgaaaaat tgtacgagt tcagccgat gaaatctcg 
      cattgtttg ttgagtgcg ttagcagcg actccgagg 
      agtaaggac tggccccgg aagagccag gatttggag 
      ctttgtgcc cggaaattg gcagcgaca catccggtt 
      ttggtcctg cgacaactc ccgatgttg gccgggagt 
      ttgaaagga agggcacag tatgactgg ggtgttttg 
      aaaaataga ggacacttg ttgctgttc ggacaagtt 
      ttggggctt ttagaactg ttggagccg atagttttc 
      gagcaaagt cgcaccttg tgtgacatt ctagattcc 
      tattttctc ctgttacaa tatcacggg catatgaaa 
      gatttgtcg gttctcgtg caaagaatt atcactttt 
      atacaaaac tggatgact aatgacatc aaggcggcg 
      tcccgctat ttgcaagag catggctca gttttactt 
      gatattcag atgattcaa ccaggggtg cgccccctt 
      ctctccaac gtgactctc ccggtgggg gattccagc 
      actcccccc gagctactc gtccgtact gccatgtcc 
      cccatgatg gaccccttg cccccacat tggtcccaa 
      ctcctcaca gctatccag acttcggag aatctcaac 
      attttgcaa gaactggac cacataaca aacaagaaa 
      ccgcatttg ctcgagaca ccgtctcaa cacttgtac 
      aattatatt agttcgtct agtggggca gtgcggtcg 
      ttggctttg acactaata gtgcggtgg ttaaaattt 
      aatccgaac gcagcatca gatgcactt ccagtgatc 
      ttaacttgt ctggatagt agaaatggt gacgtggtc 
      gcgagtgtt ttggaccga ctttcggac ctggtttca 
      gttatgcag gagtatggc aaggttatt ctgaccagg 
      gtttttcaa ttaggaatg gaatcgacg ctaaatacg 
      accagtaac attaccaaa agtattgat ttgttgaat 
      ttgcaatcg ggatgttaa
    mRNA sequence
    • >TC001242 RA
      ctgtcaaaa tttttttct tgttttgac tgcgaaaaa 
      accaaaatt gttgcattg tttgtgata attggtcca 
      ccatggatc ggaataaac cgggccaga gccaagcca 
      ctcgtggcc ccaaggcca aaataacgc aacaacccc 
      ctgacttgt tcatcgctt tgggggcca aacaatcct 
      cccctcgag atgacccca aacgtagcg ttccgctcc 
      ctaaaacca gcactgcct cccatttac aaacaagta 
      gcagtaaag tgacggata aattgaagc gtgaaggga 
      cttcgtcta gtcttacag tgccccctt cgaagaaaa 
      tgaaagttg cgataccgg gaagccggc cagggccct 
      ccactagca gcatactac ctgtggaga ggatggggg 
      ttgagccgt gggaagccc tggctgtgg actgtgaga 
      caagcgatt tgttcgaga gtattatgg cggctgtta 
      atgccggca atactgata aagctgttg ggtatgttt 
      tgggggtca ttcgctcgt tgaagactc aaaggacta 
      agatttgta aagtggttt atctgagtc ttttactga 
      cttgcagct gcaaacccc agttattca ctaatgaga 
      atatcatag cggctattg tgaatgttc tgcggcgtg 
      atgtaactt ccggtttta agggcgtta acaaaacaa 
      attcttact tccacatgc tgtttatta atttattaa 
      ctcacgcct tcaccgatg tcgcccaat ggccggaaa 
      ttttcgtca aaatttatg tggaggacg cggtggccg 
      atcgcattt ttatcgaca atccgtact gtaaaccat 
      tcgttgata acataatca cggcgttta acaccaaat 
      tgccccctt cctcgctcc tcaaatccg agacatgga 
      attccacgg ctagagaca cttctagcc ccctcacaa 
      tcacaagcg ctgacgacg acaccaaaa gcgaagtga 
      ttctcgagc ataaagccg cagttgaaa actggaacg 
      tcaaagtgt atccccggt tcacccaaa cgcaggaaa 
      acgtcgaaa aaatcgtcc ttgaagcca tcaaggaac 
      aactagcaa ggcgccaac aaccggaaa ccatcacga 
      aaaacttcg tccgcttct tgagtatcg cttcaggac 
      ttgtggaaa ttcgcataa ttgccgttt cgcgcatcg 
      agagttggc tacacaacc ataagttga tgaaatcag 
      cgcaggaac tcctcgcct atttgtgct acaattgct 
      cggcgagtt cgcaaagag acttggaaa taatagcgc 
      agctgagca agctcagac tgaagaaca aacccatgg 
      ttaactact tcaacaact gtctccggg agatggttt 
      actcgttcc cggagaact tgtaccccc ttttgaaat 
      acacgattt acaacgaac tgtccaatg cccgaaatt 
      ccaacaatt taatcgtcg tgggggccc tgtttcagg 
      tcgcacctg aggcaagtg ccgacgctt tcgccgaca 
      tttgcctgg agttgctcc taaacaaag acgactatt 
      tgcgttcgc tgcgggcgc ttttgaaag aaatcaccc 
      gagtcctcc ggcacgatt tcaacttgt taagcatcg 
      tgcattcgc tcgttcgcg agcgcaaag agtttgcga 
      ataacgtgc gcgagtcgg aatttcgcg agcgaattt 
      ttctatcac tggccgacc tggtctgca tgtgtatgc 
      tgctgtgcg tcagccctc aggtccgcg acgcctcaa 
      cgcaaaaca agcgcgacg tcacggtcc tgaaagcct 
      tccagaaac aagtcagta acatacagc gggaggcag 
      tgacttggc tcaacgact ccgcgctaa gggttttcc 
      ggccaagtc tcaacgact tccaccacg ttctactca 
      aagtcttgt tcctcgaac aagcggagc attactaca 
      aggtcgact cgtggcctg gcgaaaacg agaggaact 
      tgtttttga ggcttacga gtgaggttc ccttgcttc 
      aggcgactc ttttgagga ttttgctga tcgggaact 
      cgaaagaac acccgatta gtcccacgg aagcgattg 
      aaattagcg atcagttga ttagaaggg cggcgaatt 
      tgccccaag actgcgccc cgcccttgg tcgttgata 
      atttggaga taatcggga tttatttca acttgtgca 
      gctataatt atccggaga atatcacgc tgccggcgg 
      ggtatgtgc cccccagtt tggccattt ctgggctct 
      attggaaag tttggctca ttttgctca taatagcgg 
      ctcataatc cggtcactt ttgggaacg aggtctgga 
      acaagtacc caacgttgc gcatgttca tggaaatgt 
      gcattacaa atcactttt cgtttcccc cgcccacca 
      tgacctccc tggacgaag actaccagt cccgcgagc 
      agcaaatcc tcgcccttg agaagcaaa aaatcctgg 
      aatttgagt cacacctcg cggccgcga gcactaaaa 
      tggaaatca ccgaacaga gcagcctcc tattgcccc 
      aattaatgg aactgcggc ctgagggcg aagcccgaa 
      ggccccccc aagccgtcc tcgaccaac tccaacact 
      tgaacaaca cgcatcgca tgggacatt tattgtgcc 
      gctcgcgga accccgatt ttctattgg acattatgt 
      cgcggcaag ggggcaccg cgcatatgc cgtggctgg 
      ccgagctgg tccacaatt cggaggggg cgttggccc 
      acctgcctg tgcaatgtc tgtgcgagt atctcctgt 
      cgacgacac cgacggaga aattgacga aacatggcc 
      agcttttgg cgcatttgc ggacggtgg ttaatgaga 
      atgatccgc agacggcct gtgaggttt tggagtatt 
      tgttcaggc ggttgacgt ccgatcatg gggcgagtc 
      ggacgcagg ccaccaaag gcttgaatt tgattttgg 
      ccgaagggg aggaaagcg agacgaata atacgaact 
      ggttgacga gttacataa cacattttc cacacttta 
      cgatgatta aaccagtgc ttatacagt ttttgcggc 
      aagccttgc aaattgaga caaatccgg cgcgggttt 
      cgagttata tcaattttt tggcaacgc aggattgta 
      acgattcgt ttacggaac tgcacgaat taattgccg 
      atttatcgt cggttatta tcgagagac acagcgtgg 
      cttcgtacg tgttgccag ggccggaca accaaactt 
      tgaagcacc ttttgacca tttttgcga gtcatattc 
      agaaagcgc aggaattct cggaggata tttacagtt 
      cggtgcaaa actacagcg agtatgttt tggtgactt 
      gggtgactg gggaacaat gccacatgc agactttag 
      taatacacg cagctattg tgttactga cttacggtc 
      ctttggaga attttgagc cgttcgaga ctttgttaa 
      atttttggt ttccggcga acgccgaac aaccacaag 
      cctacacac ctgatacaa acgaacgaa cgtcgtatc 
      tccctgatt ggatgaaac tgcggatga ttcggtcaa 
      acgtcccac gattggttg acgcagcca ttgaaaaat 
      tagagccgc caaagttgg tccttttta tacaatcgt 
      tcggaatac cgacaaatt ccatcagta aattgctga 
      gtactttgg acaaagcca cacttttgg acccgaaac 
      tggtcgtgg actcagttt tggacaaaa cttacatga 
      ttcaattgg tggaagtcc aaaacaaaa gaggcgcag 
      tgggtggtg acacctttg tcaaggcta tcgagatgc 
      aaatgcccg ttgtacaag acattgatg ttaaaatcg 
      aaatcgaga gtaaaaaac aattaccca acttagtaa 
      aaacccaac agattgtca caaatagcg gcgatatta 
      tcaatttgt tgaataata tttttagcc caaactatg 
      gcggttcga aagatcggg acattgtca ctgtgacca 
      agatggcgt ttgagaaac aatcactgg acgttattt 
      tcaattatt tgaaatcac taccgatga atttcatag 
      cacctgtgc tccataaac acaattcga tttcaacgc 
      ttttccgcc tgttatttt cgtcaaaag accacctca 
      ttgacaaaa taaccatgg ctacggttt tcctcaacc 
      acatgacag acgttcaca atattactt attcggttt 
      tgaggcaat tcttaaacg aaaacgacc aaaaagtca 
      aaactgaag aacaaggcg aacggaaat tggtaagta 
      tgatatcgg aggcgaaaa cacaggaag aaaaaactg 
      acatgatgg ttgactggt tatcaacaa tccaactgg 
      aagtggctt actcgcaaa aacaattga aagaagatt 
      tgttgtttt cgcgcgaca acttgccgt ttaggccgc 
      ttcttatgt cgctggttt tgcaaaggg cgagttggc 
      agagcctcc acgtgataa tgaattatt tgctaaaag 
      aggacaatt cggacattt gccctgttt ccgcccttg 
      atttcctct gtgccctca cccagagcc cgaagctgt 
      ggcaggggc gcgataaag ccattccca aacaccacc 
      attcggagg acgtttttg atttgggca aagacaagg 
      ccctgatcc ttgtcggtt atattttgg aggaaagca 
      agcaaaacc aggcaaact ggtcgaaga aaatggaat 
      cgcggctcg tcctcttac tgcagtgta tccgaaact 
      gcgccccgg agattttgg ggacacttt tggagaagt 
      ccgactgtg atacgcact gtcgggagc ttctcttga 
      tgatttaca tgtcgctgc cgtattccg gtcaagatt 
      tgatcaata gcaaagata acatttcga agcaggcgt 
      atgcgaaaa attgtacga gttcagccg atgaaatct 
      cgcattgtt tgttgagtg cgttagcag cgactccga 
      ggagtaagg actggcccc ggaagagcc aggatttgg 
      agctttgtg cccggaaat tggcagcga cacatccgg 
      ttttggtcc tgcgacaac tcccgatgt tggccggga 
      gtttgaaag gaagggcac agtatgact ggggtgttt 
      tgaaaaata gaggacact tgttgctgt tcggacaag 
      ttttggggc ttttagaac tgttggagc cgatagttt 
      tcgagcaaa gtcgcacct tgtgtgaca ttctagatt 
      cctattttc tcctgttac aatatcacg ggcatatga 
      aagatttgt cggttctcg tgcaaagaa ttatcactt 
      ttatacaaa actggatga ctaatgaca tcaaggcgg 
      cgtcccgct atttgcaag agcatggct cagttttac 
      ttgatattc agatgattc aaccagggg tgcgccccc 
      ttctctcca acgtgactc tcccggtgg gggattcca 
      gcactcccc ccgagctac tcgtccgta ctgccatgt 
      cccccatga tggacccct tgcccccac attggtccc 
      aactcctca cagctatcc agacttcgg agaatctca 
      acattttgc aagaactgg accacataa caaacaaga 
      aaccgcatt tgctcgaga caccgtctc aacacttgt 
      acaattata ttagttcgt ctagtgggg cagtgcggt 
      cgttggctt tgacactaa tagtgcggt ggttaaaat 
      ttaatccga acgcagcat cagatgcac ttccagtga 
      tcttaactt gtctggata gtagaaatg gtgacgtgg 
      tcgcgagtg ttttggacc gactttcgg acctggttt 
      cagttatgc aggagtatg gcaaggtta ttctgacca 
      gggtttttc aattaggaa tggaatcga cgctaaata 
      cgaccagta acattacca aaagtattg atttgttga 
      atttgcaat cgggatgtt aagtaggtc tgatttatt 
      ttattaaat ataaaaatt aaatatcc