Status
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Gene information
General information
TC001230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13008328..13014159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001230
    iBeetle screen iB_06820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03158

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen dorsal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen ventral irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001230 RA
      MDGAYDLDT MMGYLGDFG RYQMWQFSL HILSAVTAG 
      LHMLSLVTV AAVPEHRCE IPGVDFNYT MTKLNSSEL 
      ASYIPKLPS GEFDSCSLL NSTTNVSYR CDSWVYDYT 
      YYKTSRAIE WSFVCDNRW MGAVAQAMY MFGVFTGAV 
      TLGNMADKF GRKPVFCWS ALLQLVIGV AVAFTPDYY 
      SFLALRYLY GIFGSAGAY IPGFVLTME LVGPSKRSM 
      CGVAFQAAF ACGIMMVAG WGALIKDRQ LLQVVYGLH 
      GLLLIGHYW LMDESPRWL WANGRSRDA VEIVKKALK 
      MNKSPINLE AAEFVSKGT AESRTKTED SVGVLDLFK 
      TPNLRIKTL NVCLNWFAN SLVYYGLSL NTGQLSGDP 
      FLILFVMGL VELPSYVLT VYLMDRMGR RSLTALNMI 
      LGGICCIAA ANLTMGSTE STAFVVTGK FLIASSFAI 
      IYNYSAELF PTVVRNSAL GIGAMCART SGALTPLIT 
      LLDSFDPTL PSIIFAVIA LISGFLTLF LPETLNKPM 
      PQTIQDGEE FGKGDTFFT SCCKKKTPD EEETVTSKP 
      PTSEQMQPL GVTR
    CDS sequence
    • >TC001230 RA
      atggacggg gcctacgat ttagacacc atgatgggc 
      tatctgggt gacttcggt cgctaccag atgtggcaa 
      ttcagcctt cacatcctc tcagcagtc actgccggc 
      ctccatatg ctttccctt gtaacagtg gctgcagtg 
      cctgagcac agatgtgag attccaggc gtcgatttc 
      aattacaca atgacgaaa ttaaattcg tcggaactt 
      gcttcatac attcccaaa cttccttcg ggagagttt 
      gatagttgt agtctgctg aacagtacg acaaatgtg 
      tcgtatcgg tgtgactcc tgggtgtac gactacacg 
      tattataag acttccaga gcgattgaa tggagcttc 
      gtctgtgat aatagatgg atgggggct gtggcccaa 
      gccatgtac atgtttggg gttttcacc ggggctgta 
      accctcgga aatatggcc gataagttc ggacgcaag 
      ccagttttt tgctggtcg gctttattg caactagta 
      ataggagtt gcggttgct tttacccct gattattac 
      tcatttttg gcgctgaga tacttgtat gggattttc 
      ggatcagcg ggggcttat ataccaggt tttgtgttg 
      accatggag ctggtgggc cccagcaag cgctccatg 
      tgtggtgta gccttccag gcagccttc gcctgtggc 
      attatgatg gtggcaggc tggggggcc ctcatcaaa 
      gaccgccaa ttgctccaa gtcgtgtac ggactccac 
      ggtttgctc ctcattggc cactactgg ctcatggac 
      gagtcaccc agatggtta tgggccaat gggcgttca 
      cgtgacgcc gtcgaaatc gtcaaaaag gcgctcaaa 
      atgaacaaa agtcccatt aatctcgaa gccgccgaa 
      ttcgtatcc aaaggaacg gccgaaagt cgcaccaaa 
      accgaagac tcagtcggg gttctagac cttttcaaa 
      acgcccaac ttgcgaatc aaaacgttg aatgtttgt 
      ctgaactgg ttcgccaat tcgttggtt tactacgga 
      ctgtcgctg aacacgggg cagttgagc ggcgatccg 
      tttttaatt ttatttgtc atgggtttg gtcgaattg 
      cccagctat gtcctaact gtgtatttg atggacaga 
      atgggtcgt cggtcctta accgccctc aatatgatc 
      cttggcggg atttgttgc atcgctgcc gcgaatcta 
      accatggga agtaccgaa tcgaccgct ttcgtcgtc 
      actggcaaa ttcctaatc gccagttct ttcgcaatt 
      atttacaat tactcggcc gaacttttc ccgactgtt 
      gtccgaaac tcggccctc ggaatcggg gcaatgtgt 
      gcccggact tccggagcc ctaactccc cttataaca 
      ctcctggat tcgttcgat ccaacccta ccgtccatt 
      attttcgca gttattgcg ctaatttcc gggtttttg 
      accttattc cttccggaa actttgaac aaacccatg 
      ccgcaaact atacaagac ggcgaagaa ttcgggaaa 
      ggcgatacg ttcttcacg tcatgttgc aagaagaag 
      actccggat gaggaagaa actgtcact tccaagcca 
      ccaacttcc gagcaaatg caaccgctg ggggtcacc 
      aggtga
    mRNA sequence
    • >TC001230 RA
      tataaacag agaaaaaat taggaaatt gtagttttg 
      ccaaacgat ggggaccaa caaagcaaa ggcttttag 
      tacacattt cgatgatca tttttattg acgtttgaa 
      ctctccgac tagttttaa aaatagtgt tagtttgca 
      gaagtggaa gtgagttat cgagacgaa tacactttg 
      tgtatgtga gtcatttaa cagaaatac gattttatt 
      ggatgtgac aggtgcaga aggaaataa aaatgaacg 
      gaaataaga aagcataga aaaaccacg tgcttttgg 
      caccaacac gtgacactt tttccgacc acatttggt 
      gtattatct tgttggaga agcgcagtc gcttggaac 
      acatgaaat gtaaaagag tgtggtgtg tggagaaga 
      cttttgttc tgtgagtcg gaagcacca gcgagatgg 
      acggggcct acgatttag acaccatga tgggctatc 
      tgggtgact tcggtcgct accagatgt ggcaattca 
      gccttcaca tcctctcag cagtcactg ccggcctcc 
      atatgcttt cccttgtaa cagtggctg cagtgcctg 
      agcacagat gtgagattc caggcgtcg atttcaatt 
      acacaatga cgaaattaa attcgtcgg aacttgctt 
      catacattc ccaaacttc cttcgggag agtttgata 
      gttgtagtc tgctgaaca gtacgacaa atgtgtcgt 
      atcggtgtg actcctggg tgtacgact acacgtatt 
      ataagactt ccagagcga ttgaatgga gcttcgtct 
      gtgataata gatggatgg gggctgtgg cccaagcca 
      tgtacatgt ttggggttt tcaccgggg ctgtaaccc 
      tcggaaata tggccgata agttcggac gcaagccag 
      ttttttgct ggtcggctt tattgcaac tagtaatag 
      gagttgcgg ttgctttta cccctgatt attactcat 
      ttttggcgc tgagatact tgtatggga ttttcggat 
      cagcggggg cttatatac caggttttg tgttgacca 
      tggagctgg tgggcccca gcaagcgct ccatgtgtg 
      gtgtagcct tccaggcag ccttcgcct gtggcatta 
      tgatggtgg caggctggg gggccctca tcaaagacc 
      gccaattgc tccaagtcg tgtacggac tccacggtt 
      tgctcctca ttggccact actggctca tggacgagt 
      cacccagat ggttatggg ccaatgggc gttcacgtg 
      acgccgtcg aaatcgtca aaaaggcgc tcaaaatga 
      acaaaagtc ccattaatc tcgaagccg ccgaattcg 
      tatccaaag gaacggccg aaagtcgca ccaaaaccg 
      aagactcag tcggggttc tagaccttt tcaaaacgc 
      ccaacttgc gaatcaaaa cgttgaatg tttgtctga 
      actggttcg ccaattcgt tggtttact acggactgt 
      cgctgaaca cggggcagt tgagcggcg atccgtttt 
      taattttat ttgtcatgg gtttggtcg aattgccca 
      gctatgtcc taactgtgt atttgatgg acagaatgg 
      gtcgtcggt ccttaaccg ccctcaata tgatccttg 
      gcgggattt gttgcatcg ctgccgcga atctaacca 
      tgggaagta ccgaatcga ccgctttcg tcgtcactg 
      gcaaattcc taatcgcca gttctttcg caattattt 
      acaattact cggccgaac ttttcccga ctgttgtcc 
      gaaactcgg ccctcggaa tcggggcaa tgtgtgccc 
      ggacttccg gagccctaa ctcccctta taacactcc 
      tggattcgt tcgatccaa ccctaccgt ccattattt 
      tcgcagtta ttgcgctaa tttccgggt ttttgacct 
      tattccttc cggaaactt tgaacaaac ccatgccgc 
      aaactatac aagacggcg aagaattcg ggaaaggcg 
      atacgttct tcacgtcat gttgcaaga agaagactc 
      cggatgagg aagaaactg tcacttcca agccaccaa 
      cttccgagc aaatgcaac cgctggggg tcaccaggt 
      gaagcgcgg ctttagcga ctgttagtg tagtaggag 
      tgtggactt attgtctat ttattcata aaataaata 
      tttttataa aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY