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Gene information
General information
TC001227 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7481806..7483449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001227
    iBeetle screen iB_03157
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001227 RA
      MGCLGECWD VGPPPDSIL SLPPPPIPA FLQHPLPDL 
      LLNTTPCSS QQLCESLPS SNRIPDAGD YVEMSRKDI 
      ESWSVTAID DTWLLVLVA SSIGVLLLG ALLAMFLLK 
      CREMNMFDG NECSLHSRD HRQIKSHEP RECTSVANL 
      NGNKLVHPS EAVMFHGGG VPDNRMVWA ALTPHGTQH 
      FISENYPRD LIDYGDVDD HYETVDNLN IEPVLSQPP 
      YYKDYDYEE PTPLIESYQ LDDMDRDGQ YQVVGNTDM 
      RCASLGFGA NTLRRGVPV RPRVSSPTR IEHPNLPPL 
      NLYPHKGGT LKRNTLSYR NVDTGTLPK FGC
    CDS sequence
    • >TC001227 RA
      atgggctgt ttgggcgaa tgctgggac gtgggccca 
      cccccggac agcatccta agcctaccg ccgccccca 
      ataccagct ttcttgcaa catccccta cccgatttg 
      ttgctaaac acgactccg tgctcctca caacaactg 
      tgcgagtct ttgccctcc tcgaacagg atcccggat 
      gctggcgac tacgtggag atgtcgagg aaagatatt 
      gagtcgtgg tcggtgacc gccatcgac gacacctgg 
      ctgctggtc ctggtggcc tcctccatc ggggtgctt 
      ctgctgggg gccctcctg gcgatgttt ctgctcaaa 
      tgccgagaa atgaacatg tttgacggc aacgagtgt 
      tcgctgcat tcgcgcgac caccgccag atcaagagc 
      cacgagccg cgcgagtgc acctccgtg gccaacctc 
      aacggcaac aagctggtg cacccctcg gaggccgtg 
      atgttccac gggggcggc gtgcccgac aaccgcatg 
      gtctgggcg gcgctcacg ccccacggc acccagcac 
      ttcatctcc gaaaactac ccccgcgat ttaatcgac 
      tatggggac gttgacgac cattacgaa accgtggac 
      aatctgaat atcgagcca gtgctctcg caaccgcct 
      tactacaaa gattacgac tacgaggaa ccgacgcct 
      ctgattgag agctaccag ctggatgac atggacagg 
      gacgggcag taccaagtg gtgggcaat acggatatg 
      aggtgtgcg agtttgggc ttcggggcg aatacgctc 
      aggaggggg gtgccggtg cggccgagg gtgtcttca 
      ccgacgcgc atcgagcac cccaatctg cccccgctt 
      aatctctac ccccacaaa gggggcacc ttgaagagg 
      aacacgctc agttatcgg aatgtcgat accggtact 
      ttgcccaag ttcgggtgc taa
    mRNA sequence
    • >TC001227 RA
      taccaaaaa taacaacag acgaacagg tgagcccca 
      tttagccta tcacttgtt tactcaaaa tattttcct 
      cccgataat cgttatcga gagtcctac ttgaatccg 
      tgtcaggtt caaaacctc ttctagaaa attcatctc 
      gtttcagat gggctgttt gggcgaatg ctgggacgt 
      gggcccacc cccggacag catcctaag cctaccgcc 
      gcccccaat accagcttt cttgcaaca tcccctacc 
      cgatttgtt gctaaacac gactccgtg ctcctcaca 
      acaactgtg cgagtcttt gccctcctc gaacaggat 
      cccggatgc tggcgacta cgtggagat gtcgaggaa 
      agatattga gtcgtggtc ggtgaccgc catcgacga 
      cacctggct gctggtcct ggtggcctc ctccatcgg 
      ggtgcttct gctgggggc cctcctggc gatgtttct 
      gctcaaatg ccgagaaat gaacatgtt tgacggcaa 
      cgagtgttc gctgcattc gcgcgacca ccgccagat 
      caagagcca cgagccgcg cgagtgcac ctccgtggc 
      caacctcaa cggcaacaa gctggtgca cccctcgga 
      ggccgtgat gttccacgg gggcggcgt gcccgacaa 
      ccgcatggt ctgggcggc gctcacgcc ccacggcac 
      ccagcactt catctccga aaactaccc ccgcgattt 
      aatcgacta tggggacgt tgacgacca ttacgaaac 
      cgtggacaa tctgaatat cgagccagt gctctcgca 
      accgcctta ctacaaaga ttacgacta cgaggaacc 
      gacgcctct gattgagag ctaccagct ggatgacat 
      ggacaggga cgggcagta ccaagtggt gggcaatac 
      ggatatgag gtgtgcgag tttgggctt cggggcgaa 
      tacgctcag gaggggggt gccggtgcg gccgagggt 
      gtcttcacc gacgcgcat cgagcaccc caatctgcc 
      cccgcttaa tctctaccc ccacaaagg gggcacctt 
      gaagaggaa cacgctcag ttatcggaa tgtcgatac 
      cggtacttt gcccaagtt cgggtgcta agtggctat 
      ttatccgca atttgtata ttgtttaca tgtaacttg 
      tctattctc gatgttttt tgattagat gctgtttat 
      ttcaataaa gtatttgtt aatgt