Status
Please wait ...
Gene information
General information
TC001207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12667205..12688587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001207
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head seta pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    vertex triplet vertex triplet posterior not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet gena triplet dorsal not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen seta random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001207 RA
      MPASSLFSD MDRGIGDER ALQLALELS MLGFSEPLT 
      AVSEPDAPA DLAPFVGSL ATVGLDEVR SKKSQNMTE 
      CVPVPSSEH VAEIVGRQG CKIKALRAK TNTYIKTPV 
      RGEEPVFVV TGRKEDVAK AKREILSAA EHFSQIRAS 
      RKNNLAGLG SGASTPPGP PANIPGHVT IQVRVPYRV 
      VGLVVGPKG ATIKRIQHQ THTYIVTPS RDKEPVFEV 
      TGLPESVES ARREIEAHI AMRTGNGAS MGLGGLDDG 
      DLFSSLYKS GLSSILNYI EPEQFPMTG SSTFSSSGS 
      CSSSSSSSG GRDLGAIWS SSERDEGLG DSPSFDSST 
      ALSSIWSYP PVAPSRPSN STSPADSLL GSAKCLVCG 
      EAKVTHALV PCGHNFFCM ECANRVCDG TEAQCPVCS 
      LPAIQAIRI YSPNP
    CDS sequence
    • >TC001207 RA
      atgccggcg tcgagcctc ttctccgac atggaccgc 
      ggcatcggc gacgagcgg gcactgcag ctggccctc 
      gagctctcc atgctgggc ttcagcgag cccttgacc 
      gccgtctcg gagcccgac gcgcccgcg gacctcgcg 
      cccttcgtg ggcagcctg gccacagtg ggcctggac 
      gaagtgcgc tccaagaag tcgcagaac atgaccgag 
      tgcgtgccg gtgcccagt tcagaacat gtggccgag 
      atcgttggc agacaaggt tgcaagatc aaggctctc 
      cgggctaaa accaataca tacataaag actccagtc 
      cgcggtgaa gaacccgtt ttcgtggta accgggcgg 
      aaagaagac gttgccaaa gccaaacgc gagattctc 
      tcagcagcc gaacacttc tcccaaatc cgcgcttcc 
      cgaaagaac aacctggcc ggtctcggg tcgggcgcg 
      agcactccc ccaggtccc ccggccaac atccccggg 
      cacgtgacg atccaggtg cgcgtgccg taccgcgtg 
      gtggggctg gtggtgggc cccaagggc gccaccatc 
      aagcgcatc cagcaccag acgcacacc tacatcgtg 
      acgcccagc cgcgacaag gagcccgtc ttcgaggtg 
      acgggcctg cccgagagc gtggagtcg gcgcggcgc 
      gagatcgag gcgcacatc gccatgcgc accggcaac 
      ggcgcctcc atgggcctg gggggcctc gacgacggc 
      gacctcttc tcctcgctg tacaagtcc ggcttgagc 
      tccatcctc aactacatc gagcccgag cagttcccc 
      atgaccggc tccagcacg ttctcgtcg tcggggtcg 
      tgctccagc agctcgtcg agcagcggc ggccgcgac 
      ctgggcgcc atctggagc agcagcgag cgcgacgag 
      ggcctgggg gactccccc agcttcgac agctcgacg 
      gcgctgtcg agcatctgg tcgtacccg ccggtggcg 
      ccgtcgcgg ccctccaac agcaccagc ccggcggac 
      tcgctgctc ggctcggcc aagtgcctc gtctgcggc 
      gaggcgaag gtcacgcac gcgttggtg ccctgcgga 
      cacaacttc ttctgcatg gagtgcgcg aaccgcgtc 
      tgcgacggc acggaggcc cagtgcccc gtctgctcc 
      ttgcccgcc atccaggcc atccgcatc tactcgccc 
      aacccttaa
    mRNA sequence
    • >TC001207 RA
      gggcacatg tctcacgcc gggccctcg ttcagtcag 
      tctcttgca agcggcgcg tctgcttgt tccacaata 
      acggttaga ccctaaact agaaattac tatattttt 
      gtacgagtg aatcgtttt tactactta gcgtcgttt 
      atatgtctt atgaacctt gttgttgcg cctgaagcc 
      ggtcgacaa tcagttctg ggagcggaa atgccggcg 
      tcgagcctc ttctccgac atggaccgc ggcatcggc 
      gacgagcgg gcactgcag ctggccctc gagctctcc 
      atgctgggc ttcagcgag cccttgacc gccgtctcg 
      gagcccgac gcgcccgcg gacctcgcg cccttcgtg 
      ggcagcctg gccacagtg ggcctggac gaagtgcgc 
      tccaagaag tcgcagaac atgaccgag tgcgtgccg 
      gtgcccagt tcagaacat gtggccgag atcgttggc 
      agacaaggt tgcaagatc aaggctctc cgggctaaa 
      accaataca tacataaag actccagtc cgcggtgaa 
      gaacccgtt ttcgtggta accgggcgg aaagaagac 
      gttgccaaa gccaaacgc gagattctc tcagcagcc 
      gaacacttc tcccaaatc cgcgcttcc cgaaagaac 
      aacctggcc ggtctcggg tcgggcgcg agcactccc 
      ccaggtccc ccggccaac atccccggg cacgtgacg 
      atccaggtg cgcgtgccg taccgcgtg gtggggctg 
      gtggtgggc cccaagggc gccaccatc aagcgcatc 
      cagcaccag acgcacacc tacatcgtg acgcccagc 
      cgcgacaag gagcccgtc ttcgaggtg acgggcctg 
      cccgagagc gtggagtcg gcgcggcgc gagatcgag 
      gcgcacatc gccatgcgc accggcaac ggcgcctcc 
      atgggcctg gggggcctc gacgacggc gacctcttc 
      tcctcgctg tacaagtcc ggcttgagc tccatcctc 
      aactacatc gagcccgag cagttcccc atgaccggc 
      tccagcacg ttctcgtcg tcggggtcg tgctccagc 
      agctcgtcg agcagcggc ggccgcgac ctgggcgcc 
      atctggagc agcagcgag cgcgacgag ggcctgggg 
      gactccccc agcttcgac agctcgacg gcgctgtcg 
      agcatctgg tcgtacccg ccggtggcg ccgtcgcgg 
      ccctccaac agcaccagc ccggcggac tcgctgctc 
      ggctcggcc aagtgcctc gtctgcggc gaggcgaag 
      gtcacgcac gcgttggtg ccctgcgga cacaacttc 
      ttctgcatg gagtgcgcg aaccgcgtc tgcgacggc 
      acggaggcc cagtgcccc gtctgctcc ttgcccgcc 
      atccaggcc atccgcatc tactcgccc aacccttaa 
      atcgatcaa tcccaccac attcccgcg ccctgtgtt 
      cagattcgg tcgggttcg cgcccttcg cttcgcacg 
      tcgcataca acgaacgct tggtttatt ttttcatag 
      gattatttt tgagttttt tcggttgtg cggcgcgca 
      gggggccaa ctcgccgaa acgtagtca tcatatgcc 
      agttactat ttttcttaa cgtcaccgt ttttctagt 
      attagacct aagattggt tggttttta ttattttgt 
      attttttat gtgtatgca cttacggaa taacctctc 
      gactccgtt aatacccta gatttgaaa agacatgac 
      gcgacacta ataaacgta aatgtaaat ttttatact 
      attgataag tggttcgtt attttttaa atatatttt 
      tttaagttt cgccatgtg acaagtcac attattaaa 
      gtctgaatc agcaattct caaaatatt atttaagtg 
      tatatttaa agtttaaaa ataatatat aaaagtaaa 
      aaaaggcaa aaaatatat attattatt gaaaaaaaa 
      gtgagaaag gacaggaga atgtattag aaggagttt 
      ggtattaaa gtgtgcagg cagggcttg ggggccttt 
      cggctgtga tctgaactg ccacacggc gtgtcggcg 
      tcgacgtct cgccgggcc ggttgcacc cctaataac 
      gacatacat atcccttat tgttagtta atcactcat 
      tgttgctat tgttctgtt atgtcatgt gatgtgata 
      catatattt ctatatttc tgatagctt ataagttat 
      gagagtatt ttgtataat ataataata attataaat 
      gaagaagaa gatgaagag tataaatga tttacgtgt 
      gcagtccgg tgtacggcg cccacgccg ccgctgata 
      gttgtcata ctgtccatg tccacagcc actgtagtc 
      ttgtatagg gtcatcgtg caccgtcat ttcgcattt 
      tttaaacaa aactaaaca aaaaaatca tcgccgtca 
      catcaaact ttgtgcggc ccccacctg cgatctcgt 
      gcctcgatc cttcagtat tcacgtcat tcgccgccc 
      cccggcctc ctttgattt tcatatgca tttcttcct 
      gcgccctgc acccccact acccgttag gtacactgt 
      tcactgttc gaccggccc acaccgacc gccctagct 
      ttacttatt acttattat tattattaa tatatatta 
      tgtattatt attatatat atttaaata ctaagatgc 
      tatattttg tttttattt attttttta ttgtagtat 
      tttgaaaat tatcgttat tagataaat tttatatat 
      tttattatt attattgcc ctcattgcg gtcccggcg 
      ggtcggtgt gggtcggtc ggtaagcgc gggtgtccc 
      ccccgacga cttagtttg tttatacgt acacttgta 
      aataagttg ttgttttat agctattaa taccatatt 
      attattaca attattatt attttattt ttttacata 
      tttttgcgg agatattta aatatgaat gttagtaca 
      agtacatgg ccacagttg tgtgcagag tgactgttt 
      gaaatagtt taattagtt tttgattga attatattt 
      atattgata ttttaatta tatgaaaca atcctcgaa 
      gtgatttct tttgtaatt ttttattga tttttgaat 
      atgaaatgt ataagtgtg ttttctatt tagaaattt 
      ataaattag tacgatttt tattcgaag cgactgacc 
      tacgcttaa ggaaattcg cttactttg gtcgtcgtc 
      gtctagtgg acaactaaa ggctgggtg tggtattcg 
      tacatatta tttaatgaa aagaaacta tattaacct 
      ttttataag actaaacca cgctcgcat ctttggttg 
      atccttaga aacctagca aatgttatt taaggtaat 
      atttttttt ataaattaa tgctaccaa atatcgtaa 
      taaatttcg tgatttcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY