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Gene information
General information
TC001199 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7042645..7046827
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001199
    iBeetle screen iB_03154
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001199 RA
      MDSPTDGNE KNQLIPTTG QFQNPELTP GIYFYPRTP 
      TSPTPSRKS FRPGAVRKI RRRAVFKNG DCNVLQSRI 
      SKRGLRFLQ DIFTTLVDI QWRWTLIIF ALAFFVSWL 
      AFALIWWLI MFTHGDLED EHLPMNQAE SNWTPCVLN 
      IHSFTSCYL FSIETQHTI GYGVRTTTE ECPEAIFIM 
      SMQSIIGMM IQAFMVGIV FAKMTRPKL RTQTLLFSR 
      NAVICQREG HLCLMFRVG DMRKSHIIG ASIRAQLIR 
      ARKTKEGEV LSNYQTELS VTADGCDNN LFFIWPMTI 
      THKIDEESP LYHLSASDM LQDKFEIVV ILEGTVEST 
      GQTTQARSS YLATEVCSI L
    CDS sequence
    • >TC001199 RA
      atggactcg ccgaccgat ggtaacgaa aaaaaccag 
      cttattccg accaccgga cagttccag aacccggaa 
      ctgactccg gggatttac ttttacccc agaacgccc 
      acttcgcca acgcccagc agaaagtcc ttccgccct 
      ggagccgtc cgcaaaatc cggcgtcga gccgtgttc 
      aaaaacggc gactgcaac gttctacaa tcccgcatt 
      tcaaagcgc ggcctccgc ttcctgcag gacattttc 
      acaaccctt gtggacatc caatggcgt tggacgctc 
      ataattttc gcgttggca tttttcgtg tcttggctc 
      gctttcgcc ttaatctgg tggctgata atgttcaca 
      catggggac ctggaggac gagcacttg cccatgaac 
      caggccgag tccaactgg accccgtgc gtcctcaac 
      atccacagc ttcacctcg tgctatctc ttcagtatt 
      gagacccaa cacaccatc ggctatggt gtgagaacc 
      acgactgaa gagtgtcct gaagctata tttattatg 
      tcaatgcaa agtatcatc ggaatgatg attcaggcc 
      ttcatggtg gggatagtt tttgcgaaa atgacacgc 
      ccgaagtta cgaacccaa actttgctt ttttctcgc 
      aatgctgtg atttgccaa agagagggt catttgtgc 
      ctcatgttt agggttgga gatatgagg aaaagccac 
      attattggg gccagtatc agggcacag ttgataagg 
      gcgcgtaaa accaaagaa ggggaagtt ctgagcaat 
      tatcaaact gagctgagt gtcacagct gatgggtgt 
      gataacaat ttgttcttc atttggccg atgactatc 
      acacataaa atcgacgaa gagtcgccg ctttatcac 
      ctctcggct agtgatatg ttgcaggat aagttcgag 
      attgtggtg attttggaa ggaacggtc gaaagtacg 
      gggcaaacg acccaagcc aggtctagt tatttggcc 
      actgaggtt tgttcaatt ttataa
    mRNA sequence
    • >TC001199 RA
      caagataac aaatcacgt tactcactc tcttatagg 
      gagcagctc ggtttgaaa aattacaat taattcgcc 
      cgctgccgc taattgatg agggcaaat gtgtgtgta 
      aatcaaaac atttgtatt taaatttgc ccaagtatt 
      taacacttc tggcgttta taatgggat tacctataa 
      ctaaataat cagttaagt ggtaagagt tctcttaac 
      gatggactc gccgaccga tggtaacga aaaaaacca 
      gcttattcc gaccaccgg acagttcca gaacccgga 
      actgactcc ggggattta cttttaccc cagaacgcc 
      cacttcgcc aacgcccag cagaaagtc cttccgccc 
      tggagccgt ccgcaaaat ccggcgtcg agccgtgtt 
      caaaaacgg cgactgcaa cgttctaca atcccgcat 
      ttcaaagcg cggcctccg cttcctgca ggacatttt 
      cacaaccct tgtggacat ccaatggcg ttggacgct 
      cataatttt cgcgttggc atttttcgt gtcttggct 
      cgctttcgc cttaatctg gtggctgat aatgttcac 
      acatgggga cctggagga cgagcactt gcccatgaa 
      ccaggccga gtccaactg gaccccgtg cgtcctcaa 
      catccacag cttcacctc gtgctatct cttcagtat 
      tgagaccca acacaccat cggctatgg tgtgagaac 
      cacgactga agagtgtcc tgaagctat atttattat 
      gtcaatgca aagtatcat cggaatgat gattcaggc 
      cttcatggt ggggatagt ttttgcgaa aatgacacg 
      cccgaagtt acgaaccca aactttgct tttttctcg 
      caatgctgt gatttgcca aagagaggg tcatttgtg 
      cctcatgtt tagggttgg agatatgag gaaaagcca 
      cattattgg ggccagtat cagggcaca gttgataag 
      ggcgcgtaa aaccaaaga aggggaagt tctgagcaa 
      ttatcaaac tgagctgag tgtcacagc tgatgggtg 
      tgataacaa tttgttctt catttggcc gatgactat 
      cacacataa aatcgacga agagtcgcc gctttatca 
      cctctcggc tagtgatat gttgcagga taagttcga 
      gattgtggt gattttgga aggaacggt cgaaagtac 
      ggggcaaac gacccaagc caggtctag ttatttggc 
      cactgaggt ttgttcaat tttataagt gattatttt 
      tggtcaaaa aattgtcga gctcatatt cgtagctaa 
      aaaaattat tgagaattt tacaatttt aaggaaaat 
      aaattcaaa attttaaa