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Gene information
General information
TC001175 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12259459..12261648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001175
    iBeetle screen iB_03151

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001175 RA
      MSIPTVIRQ INWVRPRVL NVLRPRNFC SGEPAASSS 
      GEEKKGIIL GVYESENEL VLTKAAQKF NDHVGGKLL 
      DLVKKAGKT VKRGSTRIF NNVDREFWA VAVVGLGPE 
      KAGYNEMEA IDEGMENVR VAAGIGAQV LRKQGVDRI 
      MVEGMGFPE QAAEGSALA LWRYQENKM RENQKKTPT 
      LELFDDPDG ESFQRGLFK AESQNLARR LSDTPANQM 
      TPLHFAQET VNELCPCGI KVQVHDKDW IEQKKFSAF 
      LTVARGSCE PPVVLEISY CGGPNDQKP ILFCGKGIT 
      FDSGGLCLK KCKGMDVYK ADMCGAAAI ISAIRAASA 
      LSLPLNIEA VIPLCENMP SGMAMKCGD IVMGMNGKS 
      IMITDTDNE GRLILADAL VYGQGIYKP RLIIDVASL 
      TPGVRSALG SSASGVFSN SQTMWSQVR KAGVITGDR 
      VWRLPLWKF FSKKVTRFK SHDVNNKGQ GQGSSCLGA 
      AFLNEFISC VDWIHFDIT GVGFKSNHK VTPYYTKGR 
      MTGRPTRTL IQLLYQLSC PSEGQREL
    CDS sequence
    • >TC001175 RA
      atgtcgatt ccaacagtt ataagacaa ataaattgg 
      gtaaggcct cgagtactg aacgtttta agacctcgg 
      aatttctgt tccggtgag ccagcggcg tcttcatca 
      ggcgaagaa aagaaaggc ataatttta ggcgtatac 
      gagtctgaa aatgaattg gtattgacg aaggctgca 
      caaaaattc aacgaccat gtaggagga aaattactg 
      gatttggta aaaaaagcc ggaaagact gtaaaacgg 
      ggtagtaca aggatcttc aacaacgtc gaccgggaa 
      ttctgggcc gtggccgtg gtgggcttg ggccccgag 
      aaggcgggc tacaacgag atggaagct atcgacgag 
      ggtatggag aacgtccgc gtggccgcc ggaattggg 
      gcgcaagtc ctccggaag cagggcgtg gaccggatc 
      atggtcgaa ggcatggga ttcccggag caagcggcc 
      gaagggagc gccctcgcc ctttggagg taccaggag 
      aacaaaatg cgcgagaac cagaagaag acccccacg 
      ctggagctg ttcgacgat cccgacgga gagagcttc 
      cagcgcggg ctcttcaag gcggagtcg cagaacttg 
      gcccggcgc ttgtccgac acccccgcc aaccaaatg 
      acccctctg catttcgcc caggaaact gtcaacgag 
      ctgtgcccg tgcggcatc aaggtgcaa gtccacgac 
      aaggactgg attgagcag aagaagttc agcgcgttc 
      ctgacggtg gcacgaggc tcgtgcgag ccgccggtc 
      gtgttggag atctcgtac tgcggcggt cccaacgac 
      cagaagccg atcctgttc tgcgggaag ggcatcacg 
      tttgacagc ggtggtctc tgtttgaag aaatgcaag 
      gggatggat gtgtacaag gcggatatg tgcggagcg 
      gcggcgatt atttcggcc attcgcgcc gcctccgcg 
      ttgagtctc ccgctgaat atcgaggcg gtgattccc 
      ctgtgtgag aatatgccg agtggcatg gccatgaag 
      tgtggcgac atcgtgatg ggcatgaac ggcaagagc 
      atcatgatt acggacact gacaacgaa ggacggctg 
      attctggcc gacgcgctg gtctacggc caagggatt 
      tacaagccg aggctcatc attgacgtt gcgagtcta 
      actccgggg gtgcggtcg gccttgggg tcctccgcg 
      tctggagtt ttcagcaac agccagacg atgtggtcg 
      caagtgcgc aaagccggg gttataacc ggggaccgc 
      gtctggaga ctccctctg tggaagttc ttttcgaag 
      aaggtgacc cgcttcaag tcacacgat gtgaataac 
      aagggccaa gggcaaggc tcgtcctgt ctgggggcc 
      gctttcctc aacgagttt atcagttgc gtggactgg 
      attcatttt gatatcacc ggagttggc ttcaagagc 
      aaccataag gtcacgccg tactacacc aaagggcgg 
      atgaccggc cgtccgacg agaaccctg attcagtta 
      ctttaccaa ctttcgtgt ccttctgaa ggacaacgg 
      gaactatag
    mRNA sequence
    • >TC001175 RA
      tgtgaaaaa aattcaagt gaaggcccg taaccaata 
      aataagaaa aatattatt tgtctttcc gatctaaat 
      tattcttgt aaaatgtat atattttaa atgaacaat 
      gtcgattcc aacagttat aagacaaat aaattgggt 
      aaggcctcg agtactgaa cgttttaag acctcggaa 
      tttctgttc cggtgagcc agcggcgtc ttcatcagg 
      cgaagaaaa gaaaggcat aattttagg cgtatacga 
      gtctgaaaa tgaattggt attgacgaa ggctgcaca 
      aaaattcaa cgaccatgt aggaggaaa attactgga 
      tttggtaaa aaaagccgg aaagactgt aaaacgggg 
      tagtacaag gatcttcaa caacgtcga ccgggaatt 
      ctgggccgt ggccgtggt gggcttggg ccccgagaa 
      ggcgggcta caacgagat ggaagctat cgacgaggg 
      tatggagaa cgtccgcgt ggccgccgg aattggggc 
      gcaagtcct ccggaagca gggcgtgga ccggatcat 
      ggtcgaagg catgggatt cccggagca agcggccga 
      agggagcgc cctcgccct ttggaggta ccaggagaa 
      caaaatgcg cgagaacca gaagaagac ccccacgct 
      ggagctgtt cgacgatcc cgacggaga gagcttcca 
      gcgcgggct cttcaaggc ggagtcgca gaacttggc 
      ccggcgctt gtccgacac ccccgccaa ccaaatgac 
      ccctctgca tttcgccca ggaaactgt caacgagct 
      gtgcccgtg cggcatcaa ggtgcaagt ccacgacaa 
      ggactggat tgagcagaa gaagttcag cgcgttcct 
      gacggtggc acgaggctc gtgcgagcc gccggtcgt 
      gttggagat ctcgtactg cggcggtcc caacgacca 
      gaagccgat cctgttctg cgggaaggg catcacgtt 
      tgacagcgg tggtctctg tttgaagaa atgcaaggg 
      gatggatgt gtacaaggc ggatatgtg cggagcggc 
      ggcgattat ttcggccat tcgcgccgc ctccgcgtt 
      gagtctccc gctgaatat cgaggcggt gattcccct 
      gtgtgagaa tatgccgag tggcatggc catgaagtg 
      tggcgacat cgtgatggg catgaacgg caagagcat 
      catgattac ggacactga caacgaagg acggctgat 
      tctggccga cgcgctggt ctacggcca agggattta 
      caagccgag gctcatcat tgacgttgc gagtctaac 
      tccgggggt gcggtcggc cttggggtc ctccgcgtc 
      tggagtttt cagcaacag ccagacgat gtggtcgca 
      agtgcgcaa agccggggt tataaccgg ggaccgcgt 
      ctggagact ccctctgtg gaagttctt ttcgaagaa 
      ggtgacccg cttcaagtc acacgatgt gaataacaa 
      gggccaagg gcaaggctc gtcctgtct gggggccgc 
      tttcctcaa cgagtttat cagttgcgt ggactggat 
      tcattttga tatcaccgg agttggctt caagagcaa 
      ccataaggt cacgccgta ctacaccaa agggcggat 
      gaccggccg tccgacgag aaccctgat tcagttact 
      ttaccaact ttcgtgtcc ttctgaagg acaacggga 
      actatagac ttttttggt ggattttag aaatgggat 
      atgacccac atgaccttt aaaatttaa ttgtaataa 
      atacttagg ggaatgttt tgacttgaa tcgcaatct 
      ggtcagttg ctcaaatac gattttttt ggctttttt 
      atcataaat cctgataat gctctccct ccctctcaa 
      aaaaataaa aataacatt ccgagttat gcctaagtg 
      gtctattac atttttgta gccagttta caagaattt 
      tgaaatttg tcgtatacc aatttattg tttatttta 
      aactcaaaa actgtccta tactgtaca ttttgtatc 
      aataaaata taatgcttc ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY