| General information | |||
| TC001175 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12259459..12261648 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001175 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03151 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001175 RA
MSIPTVIRQ INWVRPRVL NVLRPRNFC SGEPAASSS GEEKKGIIL GVYESENEL VLTKAAQKF NDHVGGKLL DLVKKAGKT VKRGSTRIF NNVDREFWA VAVVGLGPE KAGYNEMEA IDEGMENVR VAAGIGAQV LRKQGVDRI MVEGMGFPE QAAEGSALA LWRYQENKM RENQKKTPT LELFDDPDG ESFQRGLFK AESQNLARR LSDTPANQM TPLHFAQET VNELCPCGI KVQVHDKDW IEQKKFSAF LTVARGSCE PPVVLEISY CGGPNDQKP ILFCGKGIT FDSGGLCLK KCKGMDVYK ADMCGAAAI ISAIRAASA LSLPLNIEA VIPLCENMP SGMAMKCGD IVMGMNGKS IMITDTDNE GRLILADAL VYGQGIYKP RLIIDVASL TPGVRSALG SSASGVFSN SQTMWSQVR KAGVITGDR VWRLPLWKF FSKKVTRFK SHDVNNKGQ GQGSSCLGA AFLNEFISC VDWIHFDIT GVGFKSNHK VTPYYTKGR MTGRPTRTL IQLLYQLSC PSEGQREL
- >TC001175 RA
atgtcgatt ccaacagtt ataagacaa ataaattgg gtaaggcct cgagtactg aacgtttta agacctcgg aatttctgt tccggtgag ccagcggcg tcttcatca ggcgaagaa aagaaaggc ataatttta ggcgtatac gagtctgaa aatgaattg gtattgacg aaggctgca caaaaattc aacgaccat gtaggagga aaattactg gatttggta aaaaaagcc ggaaagact gtaaaacgg ggtagtaca aggatcttc aacaacgtc gaccgggaa ttctgggcc gtggccgtg gtgggcttg ggccccgag aaggcgggc tacaacgag atggaagct atcgacgag ggtatggag aacgtccgc gtggccgcc ggaattggg gcgcaagtc ctccggaag cagggcgtg gaccggatc atggtcgaa ggcatggga ttcccggag caagcggcc gaagggagc gccctcgcc ctttggagg taccaggag aacaaaatg cgcgagaac cagaagaag acccccacg ctggagctg ttcgacgat cccgacgga gagagcttc cagcgcggg ctcttcaag gcggagtcg cagaacttg gcccggcgc ttgtccgac acccccgcc aaccaaatg acccctctg catttcgcc caggaaact gtcaacgag ctgtgcccg tgcggcatc aaggtgcaa gtccacgac aaggactgg attgagcag aagaagttc agcgcgttc ctgacggtg gcacgaggc tcgtgcgag ccgccggtc gtgttggag atctcgtac tgcggcggt cccaacgac cagaagccg atcctgttc tgcgggaag ggcatcacg tttgacagc ggtggtctc tgtttgaag aaatgcaag gggatggat gtgtacaag gcggatatg tgcggagcg gcggcgatt atttcggcc attcgcgcc gcctccgcg ttgagtctc ccgctgaat atcgaggcg gtgattccc ctgtgtgag aatatgccg agtggcatg gccatgaag tgtggcgac atcgtgatg ggcatgaac ggcaagagc atcatgatt acggacact gacaacgaa ggacggctg attctggcc gacgcgctg gtctacggc caagggatt tacaagccg aggctcatc attgacgtt gcgagtcta actccgggg gtgcggtcg gccttgggg tcctccgcg tctggagtt ttcagcaac agccagacg atgtggtcg caagtgcgc aaagccggg gttataacc ggggaccgc gtctggaga ctccctctg tggaagttc ttttcgaag aaggtgacc cgcttcaag tcacacgat gtgaataac aagggccaa gggcaaggc tcgtcctgt ctgggggcc gctttcctc aacgagttt atcagttgc gtggactgg attcatttt gatatcacc ggagttggc ttcaagagc aaccataag gtcacgccg tactacacc aaagggcgg atgaccggc cgtccgacg agaaccctg attcagtta ctttaccaa ctttcgtgt ccttctgaa ggacaacgg gaactatag
- >TC001175 RA
tgtgaaaaa aattcaagt gaaggcccg taaccaata aataagaaa aatattatt tgtctttcc gatctaaat tattcttgt aaaatgtat atattttaa atgaacaat gtcgattcc aacagttat aagacaaat aaattgggt aaggcctcg agtactgaa cgttttaag acctcggaa tttctgttc cggtgagcc agcggcgtc ttcatcagg cgaagaaaa gaaaggcat aattttagg cgtatacga gtctgaaaa tgaattggt attgacgaa ggctgcaca aaaattcaa cgaccatgt aggaggaaa attactgga tttggtaaa aaaagccgg aaagactgt aaaacgggg tagtacaag gatcttcaa caacgtcga ccgggaatt ctgggccgt ggccgtggt gggcttggg ccccgagaa ggcgggcta caacgagat ggaagctat cgacgaggg tatggagaa cgtccgcgt ggccgccgg aattggggc gcaagtcct ccggaagca gggcgtgga ccggatcat ggtcgaagg catgggatt cccggagca agcggccga agggagcgc cctcgccct ttggaggta ccaggagaa caaaatgcg cgagaacca gaagaagac ccccacgct ggagctgtt cgacgatcc cgacggaga gagcttcca gcgcgggct cttcaaggc ggagtcgca gaacttggc ccggcgctt gtccgacac ccccgccaa ccaaatgac ccctctgca tttcgccca ggaaactgt caacgagct gtgcccgtg cggcatcaa ggtgcaagt ccacgacaa ggactggat tgagcagaa gaagttcag cgcgttcct gacggtggc acgaggctc gtgcgagcc gccggtcgt gttggagat ctcgtactg cggcggtcc caacgacca gaagccgat cctgttctg cgggaaggg catcacgtt tgacagcgg tggtctctg tttgaagaa atgcaaggg gatggatgt gtacaaggc ggatatgtg cggagcggc ggcgattat ttcggccat tcgcgccgc ctccgcgtt gagtctccc gctgaatat cgaggcggt gattcccct gtgtgagaa tatgccgag tggcatggc catgaagtg tggcgacat cgtgatggg catgaacgg caagagcat catgattac ggacactga caacgaagg acggctgat tctggccga cgcgctggt ctacggcca agggattta caagccgag gctcatcat tgacgttgc gagtctaac tccgggggt gcggtcggc cttggggtc ctccgcgtc tggagtttt cagcaacag ccagacgat gtggtcgca agtgcgcaa agccggggt tataaccgg ggaccgcgt ctggagact ccctctgtg gaagttctt ttcgaagaa ggtgacccg cttcaagtc acacgatgt gaataacaa gggccaagg gcaaggctc gtcctgtct gggggccgc tttcctcaa cgagtttat cagttgcgt ggactggat tcattttga tatcaccgg agttggctt caagagcaa ccataaggt cacgccgta ctacaccaa agggcggat gaccggccg tccgacgag aaccctgat tcagttact ttaccaact ttcgtgtcc ttctgaagg acaacggga actatagac ttttttggt ggattttag aaatgggat atgacccac atgaccttt aaaatttaa ttgtaataa atacttagg ggaatgttt tgacttgaa tcgcaatct ggtcagttg ctcaaatac gattttttt ggctttttt atcataaat cctgataat gctctccct ccctctcaa aaaaataaa aataacatt ccgagttat gcctaagtg gtctattac atttttgta gccagttta caagaattt tgaaatttg tcgtatacc aatttattg tttatttta aactcaaaa actgtccta tactgtaca ttttgtatc aataaaata taatgcttc ga
