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Gene information
General information
TC001166 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6674986..6675956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001166
    iBeetle screen iB_03150
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Defects potentially due to problems with dorsal closure. Head is more often affected than thorax.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Defects potentially due to problems with dorsal closure. Head is more often affected than thorax.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001166 RA
      MSSIEPVVL VHGGAGTIS SDAKAAQLV QGVKKAAQA 
      GYQILKSGG SVLDAVQKS VELMENDAI FNAGLGSVL 
      NVEGEVEMD ASIMLGADL SAGAVTVVK DIKNPIALA 
      RLVMEKSDH VLLASEGAK KFALKHGIT PLAPGSLVT 
      EATREALAK WKAKQVAAL AELGTVGAV AIDSQGRLA 
      AATSTGGRE GKLAGRSSD TCMIGSGTY ADDGVGAVS 
      TTGHGKTIA KFCLAHSII KAMEGGQGA EAATKGCIE 
      KMTKKLNNT AGAITLSCR GEVGVGFST NRMSWAYQK 
      GDEVHFGVD QNQHDFEKI
    CDS sequence
    • >TC001166 RA
      atgtcgtcc attgagccc gttgtcctt gtccacggg 
      ggcgccggg accatcagc agcgatgcc aaggcggcc 
      cagctggtc caaggggtg aaaaaagcg gctcaagcc 
      ggctaccaa atcctcaaa tcggggggt agtgtccta 
      gatgccgtc caaaaaagc gtcgaattg atggaaaac 
      gacgcgatt ttcaacgcc ggacttggc tcggtcctc 
      aacgtcgaa ggcgaagtg gaaatggac gctagtata 
      atgctgggg gctgattta agcgcaggg gccgtgact 
      gtggtcaaa gacatcaaa aaccccata gctttggct 
      cgactcgta atggagaag agcgaccac gtgctactg 
      gccagcgag ggagcgaaa aaattcgct ctaaagcac 
      ggtattaca cctcttgcc cccggttcc ctggttacg 
      gaagccacc agagaagca ctagccaaa tggaaggcg 
      aaacaagtc gcagctttg gccgagttg ggcactgtg 
      ggggctgtg gcgattgat agccagggg aggctcgca 
      gctgccacc tccacaggg ggacgcgag gggaagttg 
      gccgggagg tcgagcgat acttgtatg attgggagc 
      gggacttat gcggatgat ggggttggg gcggtttcc 
      acaacgggg catgggaag accatagct aagttttgc 
      ctagcacac tctattatc aaggctatg gaagggggg 
      caaggggct gaggccgct accaagggg tgtattgag 
      aaaatgacc aagaaatta aataatact gcgggggct 
      atcacccta tcttgtcgt ggggaagtt ggggttggg 
      ttcagtact aataggatg tcatgggcg tatcagaaa 
      ggggatgag gtgcatttt ggagtggat cagaaccaa 
      cacgatttc gaaaaaata taa
    mRNA sequence
    • >TC001166 RA
      cttaattta aaactttat caaatgtcg tccattgag 
      cccgttgtc cttgtccac gggggcgcc gggaccatc 
      agcagcgat gccaaggcg gcccagctg gtccaaggg 
      gtgaaaaaa gcggctcaa gccggctac caaatcctc 
      aaatcgggg ggtagtgtc ctagatgcc gtccaaaaa 
      agcgtcgaa ttgatggaa aacgacgcg attttcaac 
      gccggactt ggctcggtc ctcaacgtc gaaggcgaa 
      gtggaaatg gacgctagt ataatgctg ggggctgat 
      ttaagcgca ggggccgtg actgtggtc aaagacatc 
      aaaaacccc atagctttg gctcgactc gtaatggag 
      aagagcgac cacgtgcta ctggccagc gagggagcg 
      aaaaaattc gctctaaag cacggtatt acacctctt 
      gcccccggt tccctggtt acggaagcc accagagaa 
      gcactagcc aaatggaag gcgaaacaa gtcgcagct 
      ttggccgag ttgggcact gtgggggct gtggcgatt 
      gatagccag gggaggctc gcagctgcc acctccaca 
      gggggacgc gaggggaag ttggccggg aggtcgagc 
      gatacttgt atgattggg agcgggact tatgcggat 
      gatggggtt ggggcggtt tccacaacg gggcatggg 
      aagaccata gctaagttt tgcctagca cactctatt 
      atcaaggct atggaaggg gggcaaggg gctgaggcc 
      gctaccaag gggtgtatt gagaaaatg accaagaaa 
      ttaaataat actgcgggg gctatcacc ctatcttgt 
      cgtggggaa gttggggtt gggttcagt actaatagg 
      atgtcatgg gcgtatcag aaaggggat gaggtgcat 
      tttggagtg gatcagaac caacacgat ttcgaaaaa 
      atataaaat tttattatt aaattaata aggaatgt