GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03148 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001157 RA
MFRQILLLF FAALVSGGS PPRLRFRPD GRIIGGDVA TIEDNPWQV SLEAFGIHN CGGSIISPN TILTAAHCI DGVISLFYE VVSGTADLL QNTTRSKVK EAIVHENYD NLSHDVALI ILAENLTFS DTTQAIPLG DEEPVAGDK VSVSGWGIL NDGDIITPN ILHSVNVTI VGREECATD YANVEGAHI DDTMVCAGV PEGGKDACS GDSGGPLTK NGILVGIVS WGLGCALPG YPGVYTNVA SVREWIRNN TGI
- >TC001157 RB
MPGLALFLI TVIAFISGT PSLDKRIIG GTFAEISTV PYQVSLQNN YGHFCGGSI IHKSYILTA AHCVDGKPV RGIKVIAGS SSLYHGGTI RDVCGAQKH PLYTKCDQG CDYDIAFDL IFNQNIFTI ALPQPWEVV RGGTSVSVT GWGITKKTI
- >TC001157 RC
MFRQILLLF FAALVSGGS PPRLRFRPD GRIIGGDVA TIEDNPWQV SLEAFGIHN CGGSIISPN TILTAAHCI DGVISLFYE VVSGTADLL QNTTRSKVK EAIVHENYD NLSHDVALI ILAENLTFS DTTQAIPLG DEEPVAGDK VSVSGWGIL NDGDIITPN ILHSVNVTI VGREECATD YANVEGAHI DDTMVCAGV PEGGKDACS GDSGGPLTK NGILVGIVS WGLGCALPG KHKFSGTPS LDKRIIGGT FAEISTVPY QVSLQNNYG HFCGGSIIH KSYILTAAH CVDGKPVRG IKVIAGSSS LYHGGTIRD VCGAQKHPL YTKCDQGCD YDIAFDLIF NQNIFTIAL PQPWEVVRG GTSVSVTGW GITKKTI
- >TC001157 RA
atgttccga caaattttg ctcttattt ttcgccgct ttagtttca ggtggtagt ccgccgagg cttagattc cgccccgat ggccgcatt atcggagga gacgtggcc acaattgaa gacaatcct tggcaagtt tccttggaa gctttcggt attcataac tgtggtggt tccattatt tctccaaac acgatttta acagcagct cactgcatt gatggcgta atttcactc ttctacgaa gtcgtttca ggaaccgcc gacttgctc caaaacacg acgcgaagc aaagtcaaa gaagcaata gtacatgaa aactacgac aatctaagt cacgatgtt gctcttatt attctagcg gaaaacttg acatttagc gacaccact caagcaatt cccctggga gacgaagaa ccagtggct ggtgacaag gtttctgtt tcaggttgg gggatttta aacgatggc gatattata actccgaat attctccat tctgttaac gttacaata gtgggtcgg gaggaatgc gctacagat tatgcaaac gttgaaggt gcacacatt gacgatact atggtctgt gctggagtt cccgaggga ggcaaggat gcctgttct ggggattcg ggaggtcct ttaaccaaa aacgggatt ttagtcggg atagtttca tggggactg gggtgcgca cttcctggg tacccagga gtttataca aacgtggct tcagtgagg gaatggata cggaacaat actggaata taa
- >TC001157 RB
atgcctggt ttggctctg ttcttgatc acagttatt gcctttatt tcagggacc ccttcctta gacaagcgt ataattggg ggaaccttt gctgaaata agcacagtc ccttatcaa gtttctctg cagaacaac tatggacac ttttgtggg ggctccata attcacaaa tcttacatt ttaactgct gctcattgt gtagatggc aaaccggtt aggggaata aaagtcata gctgggagt tcatctcta taccatgga ggaacaatt cgagatgtt tgtggtgca caaaaacac ccactgtac acgaaatgc gaccaaggt tgtgactac gatattgca ttcgatttg attttcaat caaaacatt ttcacaatt gcgttgcca caaccctgg gaagttgtt agaggcgga acttcagtt tcagtaact ggatgggga attacgaaa aaaacgatt taa
- >TC001157 RC
atgttccga caaattttg ctcttattt ttcgccgct ttagtttca ggtggtagt ccgccgagg cttagattc cgccccgat ggccgcatt atcggagga gacgtggcc acaattgaa gacaatcct tggcaagtt tccttggaa gctttcggt attcataac tgtggtggt tccattatt tctccaaac acgatttta acagcagct cactgcatt gatggcgta atttcactc ttctacgaa gtcgtttca ggaaccgcc gacttgctc caaaacacg acgcgaagc aaagtcaaa gaagcaata gtacatgaa aactacgac aatctaagt cacgatgtt gctcttatt attctagcg gaaaacttg acatttagc gacaccact caagcaatt cccctggga gacgaagaa ccagtggct ggtgacaag gtttctgtt tcaggttgg gggatttta aacgatggc gatattata actccgaat attctccat tctgttaac gttacaata gtgggtcgg gaggaatgc gctacagat tatgcaaac gttgaaggt gcacacatt gacgatact atggtctgt gctggagtt cccgaggga ggcaaggat gcctgttct ggggattcg ggaggtcct ttaaccaaa aacgggatt ttagtcggg atagtttca tggggactg gggtgcgca cttcctgga aagcacaaa ttttcaggg accccttcc ttagacaag cgtataatt gggggaacc tttgctgaa ataagcaca gtcccttat caagtttct ctgcagaac aactatgga cacttttgt gggggctcc ataattcac aaatcttac attttaact gctgctcat tgtgtagat ggcaaaccg gttagggga ataaaagtc atagctggg agttcatct ctataccat ggaggaaca attcgagat gtttgtggt gcacaaaaa cacccactg tacacgaaa tgcgaccaa ggttgtgac tacgatatt gcattcgat ttgattttc aatcaaaac attttcaca attgcgttg ccacaaccc tgggaagtt gttagaggc ggaacttca gtttcagta actggatgg ggaattacg aaaaaaacg atttaa
- >TC001157 RA
agacgaaaa tgttccgac aaattttgc tcttatttt tcgccgctt tagtttcag gtggtagtc cgccgaggc ttagattcc gccccgatg gccgcatta tcggaggag acgtggcca caattgaag acaatcctt ggcaagttt ccttggaag ctttcggta ttcataact gtggtggtt ccattattt ctccaaaca cgattttaa cagcagctc actgcattg atggcgtaa tttcactct tctacgaag tcgtttcag gaaccgccg acttgctcc aaaacacga cgcgaagca aagtcaaag aagcaatag tacatgaaa actacgaca atctaagtc acgatgttg ctcttatta ttctagcgg aaaacttga catttagcg acaccactc aagcaattc ccctgggag acgaagaac cagtggctg gtgacaagg tttctgttt caggttggg ggattttaa acgatggcg atattataa ctccgaata ttctccatt ctgttaacg ttacaatag tgggtcggg aggaatgcg ctacagatt atgcaaacg ttgaaggtg cacacattg acgatacta tggtctgtg ctggagttc ccgagggag gcaaggatg cctgttctg gggattcgg gaggtcctt taaccaaaa acgggattt tagtcggga tagtttcat ggggactgg ggtgcgcac ttcctgggt acccaggag tttatacaa acgtggctt cagtgaggg aatggatac ggaacaata ctggaatat aataaaatt gtatgccaa aataaataa aagtgattt atatat
- >TC001157 RB
aagacataa tgtttctaa tgcctggtt tggctctgt tcttgatca cagttattg cctttattt cagggaccc cttccttag acaagcgta taattgggg gaacctttg ctgaaataa gcacagtcc cttatcaag tttctctgc agaacaact atggacact tttgtgggg gctccataa ttcacaaat cttacattt taactgctg ctcattgtg tagatggca aaccggtta ggggaataa aagtcatag ctgggagtt catctctat accatggag gaacaattc gagatgttt gtggtgcac aaaaacacc cactgtaca cgaaatgcg accaaggtt gtgactacg atattgcat tcgatttga ttttcaatc aaaacattt tcacaattg cgttgccac aaccctggg aagttgtta gaggcggaa cttcagttt cagtaactg gatggggaa ttacgaaaa aaacgattt aaacctttc accctggct ccagaaagc ttttctcgt taccgttaa acaatggaa ttgtcgtcg tatgataaa aacgggaat tattactag tcgcatgct ttgtgctgg ttgcatagt tggttcatg cggttcatg tcaaggaga ttctggagg accgcttgt tcttgataa taaagtagt agggattgt
- >TC001157 RC
agacgaaaa tgttccgac aaattttgc tcttatttt tcgccgctt tagtttcag gtggtagtc cgccgaggc ttagattcc gccccgatg gccgcatta tcggaggag acgtggcca caattgaag acaatcctt ggcaagttt ccttggaag ctttcggta ttcataact gtggtggtt ccattattt ctccaaaca cgattttaa cagcagctc actgcattg atggcgtaa tttcactct tctacgaag tcgtttcag gaaccgccg acttgctcc aaaacacga cgcgaagca aagtcaaag aagcaatag tacatgaaa actacgaca atctaagtc acgatgttg ctcttatta ttctagcgg aaaacttga catttagcg acaccactc aagcaattc ccctgggag acgaagaac cagtggctg gtgacaagg tttctgttt caggttggg ggattttaa acgatggcg atattataa ctccgaata ttctccatt ctgttaacg ttacaatag tgggtcggg aggaatgcg ctacagatt atgcaaacg ttgaaggtg cacacattg acgatacta tggtctgtg ctggagttc ccgagggag gcaaggatg cctgttctg gggattcgg gaggtcctt taaccaaaa acgggattt tagtcggga tagtttcat ggggactgg ggtgcgcac ttcctggaa agcacaaat tttcaggga ccccttcct tagacaagc gtataattg ggggaacct ttgctgaaa taagcacag tcccttatc aagtttctc tgcagaaca actatggac acttttgtg ggggctcca taattcaca aatcttaca ttttaactg ctgctcatt gtgtagatg gcaaaccgg ttaggggaa taaaagtca tagctggga gttcatctc tataccatg gaggaacaa ttcgagatg tttgtggtg cacaaaaac acccactgt acacgaaat gcgaccaag gttgtgact acgatattg cattcgatt tgattttca atcaaaaca ttttcacaa ttgcgttgc cacaaccct gggaagttg ttagaggcg gaacttcag tttcagtaa ctggatggg gaattacga aaaaaacga tttaaacct ttcaccctg gctccagaa agcttttct cgttaccgt taaacaatg gaattgtcg tcgtatgat aaaaacggg aattattac tagtcgcat gctttgtgc tggttgcat agttggttc atgcggttc atgtcaagg agattctgg aggaccgct tgttcttga taataaagt agtagggat tgt
