Status
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Gene information
General information
TC001157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12101079..12103375
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001157
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001157 RA
      MFRQILLLF FAALVSGGS PPRLRFRPD GRIIGGDVA 
      TIEDNPWQV SLEAFGIHN CGGSIISPN TILTAAHCI 
      DGVISLFYE VVSGTADLL QNTTRSKVK EAIVHENYD 
      NLSHDVALI ILAENLTFS DTTQAIPLG DEEPVAGDK 
      VSVSGWGIL NDGDIITPN ILHSVNVTI VGREECATD 
      YANVEGAHI DDTMVCAGV PEGGKDACS GDSGGPLTK 
      NGILVGIVS WGLGCALPG YPGVYTNVA SVREWIRNN 
      TGI
    • >TC001157 RB
      MPGLALFLI TVIAFISGT PSLDKRIIG GTFAEISTV 
      PYQVSLQNN YGHFCGGSI IHKSYILTA AHCVDGKPV 
      RGIKVIAGS SSLYHGGTI RDVCGAQKH PLYTKCDQG 
      CDYDIAFDL IFNQNIFTI ALPQPWEVV RGGTSVSVT 
      GWGITKKTI
    • >TC001157 RC
      MFRQILLLF FAALVSGGS PPRLRFRPD GRIIGGDVA 
      TIEDNPWQV SLEAFGIHN CGGSIISPN TILTAAHCI 
      DGVISLFYE VVSGTADLL QNTTRSKVK EAIVHENYD 
      NLSHDVALI ILAENLTFS DTTQAIPLG DEEPVAGDK 
      VSVSGWGIL NDGDIITPN ILHSVNVTI VGREECATD 
      YANVEGAHI DDTMVCAGV PEGGKDACS GDSGGPLTK 
      NGILVGIVS WGLGCALPG KHKFSGTPS LDKRIIGGT 
      FAEISTVPY QVSLQNNYG HFCGGSIIH KSYILTAAH 
      CVDGKPVRG IKVIAGSSS LYHGGTIRD VCGAQKHPL 
      YTKCDQGCD YDIAFDLIF NQNIFTIAL PQPWEVVRG 
      GTSVSVTGW GITKKTI
    CDS sequence
    • >TC001157 RA
      atgttccga caaattttg ctcttattt ttcgccgct 
      ttagtttca ggtggtagt ccgccgagg cttagattc 
      cgccccgat ggccgcatt atcggagga gacgtggcc 
      acaattgaa gacaatcct tggcaagtt tccttggaa 
      gctttcggt attcataac tgtggtggt tccattatt 
      tctccaaac acgatttta acagcagct cactgcatt 
      gatggcgta atttcactc ttctacgaa gtcgtttca 
      ggaaccgcc gacttgctc caaaacacg acgcgaagc 
      aaagtcaaa gaagcaata gtacatgaa aactacgac 
      aatctaagt cacgatgtt gctcttatt attctagcg 
      gaaaacttg acatttagc gacaccact caagcaatt 
      cccctggga gacgaagaa ccagtggct ggtgacaag 
      gtttctgtt tcaggttgg gggatttta aacgatggc 
      gatattata actccgaat attctccat tctgttaac 
      gttacaata gtgggtcgg gaggaatgc gctacagat 
      tatgcaaac gttgaaggt gcacacatt gacgatact 
      atggtctgt gctggagtt cccgaggga ggcaaggat 
      gcctgttct ggggattcg ggaggtcct ttaaccaaa 
      aacgggatt ttagtcggg atagtttca tggggactg 
      gggtgcgca cttcctggg tacccagga gtttataca 
      aacgtggct tcagtgagg gaatggata cggaacaat 
      actggaata taa
    • >TC001157 RB
      atgcctggt ttggctctg ttcttgatc acagttatt 
      gcctttatt tcagggacc ccttcctta gacaagcgt 
      ataattggg ggaaccttt gctgaaata agcacagtc 
      ccttatcaa gtttctctg cagaacaac tatggacac 
      ttttgtggg ggctccata attcacaaa tcttacatt 
      ttaactgct gctcattgt gtagatggc aaaccggtt 
      aggggaata aaagtcata gctgggagt tcatctcta 
      taccatgga ggaacaatt cgagatgtt tgtggtgca 
      caaaaacac ccactgtac acgaaatgc gaccaaggt 
      tgtgactac gatattgca ttcgatttg attttcaat 
      caaaacatt ttcacaatt gcgttgcca caaccctgg 
      gaagttgtt agaggcgga acttcagtt tcagtaact 
      ggatgggga attacgaaa aaaacgatt taa
    • >TC001157 RC
      atgttccga caaattttg ctcttattt ttcgccgct 
      ttagtttca ggtggtagt ccgccgagg cttagattc 
      cgccccgat ggccgcatt atcggagga gacgtggcc 
      acaattgaa gacaatcct tggcaagtt tccttggaa 
      gctttcggt attcataac tgtggtggt tccattatt 
      tctccaaac acgatttta acagcagct cactgcatt 
      gatggcgta atttcactc ttctacgaa gtcgtttca 
      ggaaccgcc gacttgctc caaaacacg acgcgaagc 
      aaagtcaaa gaagcaata gtacatgaa aactacgac 
      aatctaagt cacgatgtt gctcttatt attctagcg 
      gaaaacttg acatttagc gacaccact caagcaatt 
      cccctggga gacgaagaa ccagtggct ggtgacaag 
      gtttctgtt tcaggttgg gggatttta aacgatggc 
      gatattata actccgaat attctccat tctgttaac 
      gttacaata gtgggtcgg gaggaatgc gctacagat 
      tatgcaaac gttgaaggt gcacacatt gacgatact 
      atggtctgt gctggagtt cccgaggga ggcaaggat 
      gcctgttct ggggattcg ggaggtcct ttaaccaaa 
      aacgggatt ttagtcggg atagtttca tggggactg 
      gggtgcgca cttcctgga aagcacaaa ttttcaggg 
      accccttcc ttagacaag cgtataatt gggggaacc 
      tttgctgaa ataagcaca gtcccttat caagtttct 
      ctgcagaac aactatgga cacttttgt gggggctcc 
      ataattcac aaatcttac attttaact gctgctcat 
      tgtgtagat ggcaaaccg gttagggga ataaaagtc 
      atagctggg agttcatct ctataccat ggaggaaca 
      attcgagat gtttgtggt gcacaaaaa cacccactg 
      tacacgaaa tgcgaccaa ggttgtgac tacgatatt 
      gcattcgat ttgattttc aatcaaaac attttcaca 
      attgcgttg ccacaaccc tgggaagtt gttagaggc 
      ggaacttca gtttcagta actggatgg ggaattacg 
      aaaaaaacg atttaa
    mRNA sequence
    • >TC001157 RA
      agacgaaaa tgttccgac aaattttgc tcttatttt 
      tcgccgctt tagtttcag gtggtagtc cgccgaggc 
      ttagattcc gccccgatg gccgcatta tcggaggag 
      acgtggcca caattgaag acaatcctt ggcaagttt 
      ccttggaag ctttcggta ttcataact gtggtggtt 
      ccattattt ctccaaaca cgattttaa cagcagctc 
      actgcattg atggcgtaa tttcactct tctacgaag 
      tcgtttcag gaaccgccg acttgctcc aaaacacga 
      cgcgaagca aagtcaaag aagcaatag tacatgaaa 
      actacgaca atctaagtc acgatgttg ctcttatta 
      ttctagcgg aaaacttga catttagcg acaccactc 
      aagcaattc ccctgggag acgaagaac cagtggctg 
      gtgacaagg tttctgttt caggttggg ggattttaa 
      acgatggcg atattataa ctccgaata ttctccatt 
      ctgttaacg ttacaatag tgggtcggg aggaatgcg 
      ctacagatt atgcaaacg ttgaaggtg cacacattg 
      acgatacta tggtctgtg ctggagttc ccgagggag 
      gcaaggatg cctgttctg gggattcgg gaggtcctt 
      taaccaaaa acgggattt tagtcggga tagtttcat 
      ggggactgg ggtgcgcac ttcctgggt acccaggag 
      tttatacaa acgtggctt cagtgaggg aatggatac 
      ggaacaata ctggaatat aataaaatt gtatgccaa 
      aataaataa aagtgattt atatat
    • >TC001157 RB
      aagacataa tgtttctaa tgcctggtt tggctctgt 
      tcttgatca cagttattg cctttattt cagggaccc 
      cttccttag acaagcgta taattgggg gaacctttg 
      ctgaaataa gcacagtcc cttatcaag tttctctgc 
      agaacaact atggacact tttgtgggg gctccataa 
      ttcacaaat cttacattt taactgctg ctcattgtg 
      tagatggca aaccggtta ggggaataa aagtcatag 
      ctgggagtt catctctat accatggag gaacaattc 
      gagatgttt gtggtgcac aaaaacacc cactgtaca 
      cgaaatgcg accaaggtt gtgactacg atattgcat 
      tcgatttga ttttcaatc aaaacattt tcacaattg 
      cgttgccac aaccctggg aagttgtta gaggcggaa 
      cttcagttt cagtaactg gatggggaa ttacgaaaa 
      aaacgattt aaacctttc accctggct ccagaaagc 
      ttttctcgt taccgttaa acaatggaa ttgtcgtcg 
      tatgataaa aacgggaat tattactag tcgcatgct 
      ttgtgctgg ttgcatagt tggttcatg cggttcatg 
      tcaaggaga ttctggagg accgcttgt tcttgataa 
      taaagtagt agggattgt
    • >TC001157 RC
      agacgaaaa tgttccgac aaattttgc tcttatttt 
      tcgccgctt tagtttcag gtggtagtc cgccgaggc 
      ttagattcc gccccgatg gccgcatta tcggaggag 
      acgtggcca caattgaag acaatcctt ggcaagttt 
      ccttggaag ctttcggta ttcataact gtggtggtt 
      ccattattt ctccaaaca cgattttaa cagcagctc 
      actgcattg atggcgtaa tttcactct tctacgaag 
      tcgtttcag gaaccgccg acttgctcc aaaacacga 
      cgcgaagca aagtcaaag aagcaatag tacatgaaa 
      actacgaca atctaagtc acgatgttg ctcttatta 
      ttctagcgg aaaacttga catttagcg acaccactc 
      aagcaattc ccctgggag acgaagaac cagtggctg 
      gtgacaagg tttctgttt caggttggg ggattttaa 
      acgatggcg atattataa ctccgaata ttctccatt 
      ctgttaacg ttacaatag tgggtcggg aggaatgcg 
      ctacagatt atgcaaacg ttgaaggtg cacacattg 
      acgatacta tggtctgtg ctggagttc ccgagggag 
      gcaaggatg cctgttctg gggattcgg gaggtcctt 
      taaccaaaa acgggattt tagtcggga tagtttcat 
      ggggactgg ggtgcgcac ttcctggaa agcacaaat 
      tttcaggga ccccttcct tagacaagc gtataattg 
      ggggaacct ttgctgaaa taagcacag tcccttatc 
      aagtttctc tgcagaaca actatggac acttttgtg 
      ggggctcca taattcaca aatcttaca ttttaactg 
      ctgctcatt gtgtagatg gcaaaccgg ttaggggaa 
      taaaagtca tagctggga gttcatctc tataccatg 
      gaggaacaa ttcgagatg tttgtggtg cacaaaaac 
      acccactgt acacgaaat gcgaccaag gttgtgact 
      acgatattg cattcgatt tgattttca atcaaaaca 
      ttttcacaa ttgcgttgc cacaaccct gggaagttg 
      ttagaggcg gaacttcag tttcagtaa ctggatggg 
      gaattacga aaaaaacga tttaaacct ttcaccctg 
      gctccagaa agcttttct cgttaccgt taaacaatg 
      gaattgtcg tcgtatgat aaaaacggg aattattac 
      tagtcgcat gctttgtgc tggttgcat agttggttc 
      atgcggttc atgtcaagg agattctgg aggaccgct 
      tgttcttga taataaagt agtagggat tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY