| General information | |||
| TC001146 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid7: 6053967..6075906 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001146 | |
| General information | ||||
| iB_03147 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001146 RA
MESAVMTPG NTRLKVRGK NKSLTRLLN RNHSLSNKN NRNARPNSL RSSSNQRHL NHFPKSVRC NQIDNAAFV NNINNGNDT EERKIIFHG NFTREVYNA ICLRLGHTL YIPEDSVQS VFNDMQLYP SKDQVSEML KCARQCGRR NGASKYLTF GEFCVFVRE MQNQNPKQN RKIQSCKSN NKCGNNCEV FLGGSCNPT TWRADTAIP ELQKHGITF YNPQVSMWV PELVAQEHD AKQAASVLL YVVDNQTRS TVGMIEVAY LVASGRCVV IVAHSYRPG QSIMGETIT QREYRDLVE GQTTLLTLV RSKGIKVHQ TLPSALQCT AKILRNVSN DMTPEEQLT MKLRKLREI FDSCGGPNG EIDSLKFMK AFQQLTQRE LTNNELYKY FTPNNKSIT FEKFCMLMA ELTSDNCDT STTNGWVSQ PFQRQCSTN NNTCNVENS VLNGTIDEP FTNTSFKNK YDVFLGGTH NSLSNWRED VAIPTLKKY NIRYNPVST NGYEVLDNK SVSDEDVLE WKQMMDGAD VLLFVITED TRSLTTMIL AAHYIAIGK NLVLCVQHL PEENCVVGN ESLSEQAIK DYNRGRVYV TDMAKRKQI PVFEDIAEA VQCVVNMIQ SR
- >TC001146 RA
atggaaagt gcagttatg actccggga aatactcga ttgaaagta cgcgggaaa aataaatcg ttaacgcgg ctcctgaac cgtaatcac agcttgagc aataaaaac aatcggaat gctagaccc aattcgttg aggagcagc tcgaaccag agacattta aaccatttt ccgaagagt gtacgctgt aatcagatc gataacgct gccttcgta aacaatatc aacaatgga aatgatact gaagagagg aaaattatt ttccatgga aattttaca agagaggtt tacaatgca atatgcctt cggttagga cacaccctt tatattcct gaagattcc gtgcaatct gtttttaac gatatgcag ctttatcca tcgaaagat caagtgtca gaaatgttg aagtgtgcg agacagtgc ggtcgacga aatggggca tctaagtat ctcaccttt ggcgagttt tgtgtcttt gttcgagaa atgcaaaat cagaatccg aagcagaac cgcaaaata cagtcatgt aaatcgaac aacaaatgt ggaaataat tgtgaggta ttcttagga gggtcatgc aatcccaca acatggaga gccgatacg gcaattcca gagctccaa aaacacggt attacgttt tataatcca caagtttcg atgtgggtg cctgaactg gtggcccag gagcacgat gctaaacag gccgcctca gttttgctc tatgtggtg gacaaccaa actagaagc actgttggc atgattgaa gtcgcgtat ttggtggct tcagggcgg tgtgttgtc attgttgca cactcgtac agacctggg caaagtatc atgggggag acaatcacg cagagggag tatagagat ttggtagag ggccagaca acgttatta acgctagtt aggagtaaa gggattaaa gtccaccaa actttgccc tcagctttg caatgcact gcgaagatt ttaagaaat gttagcaac gatatgaca cccgaagaa cagctaact atgaaactg agaaaattg cgggaaatt tttgactcc tgtggaggt ccaaacggt gaaatagat tcgttaaaa tttatgaaa gccttccag caattaact cagcgtgaa ctaacgaat aacgaactt tacaagtat tttactccc aacaacaaa agcatcaca tttgagaaa ttctgcatg ttgatggcg gaactgacc agcgacaac tgtgatacg agcacgaca aatgggtgg gttagtcag ccttttcag cggcaatgt tcgacaaat aacaacact tgtaacgtg gaaaattct gtactaaat ggtacaata gacgaaccc ttcaccaac acgtcattt aaaaataag tatgatgtg tttcttggt ggaacgcat aattcacta agcaactgg cgagaagat gtagcaata ccaacgttg aaaaaatac aatataagg tataatcca gtttctacg aacggatat gaagtatta gataataag agtgtgagt gatgaagac gtcttggag tggaaacag atgatggat ggtgccgat gtgttattg tttgtcata acggaggat acgaggtca ttgacaact atgatcttg gccgctcat tacattgca ataggaaaa aaccttgtc ttgtgtgtt cagcacttg ccagaagag aactgtgtt gttggcaat gaatctctg tcggagcag gcgataaaa gactacaat cgcgggagg gtgtacgta acagatatg gcgaaacgt aaacaaatt ccagtattt gaagacatc gcagaagct gtgcaatgt gtagtcaac atgatacag agcagatag
- >TC001146 RA
tgtcgctgg agagattag ttccaatcc acgttccga acccaatac attgtccta ctgtctgac ctcgcactt cttccaata gttgcgtag gaggactta aacgagata aataaacat tcagtgact tttgcttgt tccggtgat ttatttgcg caaagctgg cggcaccaa acgaagacg accaacaaa gtgtgttcc tgtatgtga gtgagtttg cgttgcgtc tctgcgtat ctgttcgtt catcggttc attttttac aagaatggc acgtgccac cgaatacat cacaagcca aataaacaa acatgtcga aagtgcaaa caaatatta gtgccatac accatgcaa agttcctgc acgaaatgt tggtaatca caccggtgc cgatatttt ctctatatt atttttaaa cgtacgttg gtgtctatc ctaaattta agtcgtaca tgcgaaacg tggagaaat caattttcc aaatgtcac gactctcga ggacaccac attgttagt ttgccgtta gttttggaa aatttccgc ggaaaaatg gaaagtgca gttatgact ccgggaaat actcgattg aaagtacgc gggaaaaat aaatcgtta acgcggctc ctgaaccgt aatcacagc ttgagcaat aaaaacaat cggaatgct agacccaat tcgttgagg agcagctcg aaccagaga catttaaac cattttccg aagagtgta cgctgtaat cagatcgat aacgctgcc ttcgtaaac aatatcaac aatggaaat gatactgaa gagaggaaa attattttc catggaaat tttacaaga gaggtttac aatgcaata tgccttcgg ttaggacac accctttat attcctgaa gattccgtg caatctgtt tttaacgat atgcagctt tatccatcg aaagatcaa gtgtcagaa atgttgaag tgtgcgaga cagtgcggt cgacgaaat ggggcatct aagtatctc acctttggc gagttttgt gtctttgtt cgagaaatg caaaatcag aatccgaag cagaaccgc aaaatacag tcatgtaaa tcgaacaac aaatgtgga aataattgt gaggtattc ttaggaggg tcatgcaat cccacaaca tggagagcc gatacggca attccagag ctccaaaaa cacggtatt acgttttat aatccacaa gtttcgatg tgggtgcct gaactggtg gcccaggag cacgatgct aaacaggcc gcctcagtt ttgctctat gtggtggac aaccaaact agaagcact gttggcatg attgaagtc gcgtatttg gtggcttca gggcggtgt gttgtcatt gttgcacac tcgtacaga cctgggcaa agtatcatg ggggagaca atcacgcag agggagtat agagatttg gtagagggc cagacaacg ttattaacg ctagttagg agtaaaggg attaaagtc caccaaact ttgccctca gctttgcaa tgcactgcg aagatttta agaaatgtt agcaacgat atgacaccc gaagaacag ctaactatg aaactgaga aaattgcgg gaaattttt gactcctgt ggaggtcca aacggtgaa atagattcg ttaaaattt atgaaagcc ttccagcaa ttaactcag cgtgaacta acgaataac gaactttac aagtatttt actcccaac aacaaaagc atcacattt gagaaattc tgcatgttg atggcggaa ctgaccagc gacaactgt gatacgagc acgacaaat gggtgggtt agtcagcct tttcagcgg caatgttcg acaaataac aacacttgt aacgtggaa aattctgta ctaaatggt acaatagac gaacccttc accaacacg tcatttaaa aataagtat gatgtgttt cttggtgga acgcataat tcactaagc aactggcga gaagatgta gcaatacca acgttgaaa aaatacaat ataaggtat aatccagtt tctacgaac ggatatgaa gtattagat aataagagt gtgagtgat gaagacgtc ttggagtgg aaacagatg atggatggt gccgatgtg ttattgttt gtcataacg gaggatacg aggtcattg acaactatg atcttggcc gctcattac attgcaata ggaaaaaac cttgtcttg tgtgttcag cacttgcca gaagagaac tgtgttgtt ggcaatgaa tctctgtcg gagcaggcg ataaaagac tacaatcgc gggagggtg tacgtaaca gatatggcg aaacgtaaa caaattcca gtatttgaa gacatcgca gaagctgtg caatgtgta gtcaacatg atacagagc agatagtca gatacctca cattttgtt ttataaatg aattggaat tttcatttc atgacacag gaaaaacat atttgctct cttgaacgg ttttgtaag gcagctctc ttcttgtaa agcagctcg tttcaggtg aaatggaat tttcgataa atacgcttt tatattttt attatttgt tattaatgt tttaatgta actctaatt tcattgtta ttgaacact tattggtgc agtttttta tttgtttga tcattgtaa cggaaatct aacttgtgg atgtttccc actaagata atgttagat gtaaagtgg tataatttt tattaactg aaattcttg tttaacagc tatactttg atatgtaca gtgtaaaaa gtgatgaag gtagctaat gttggtaaa gacaagttc ctctctcaa atgtaaata cggctgcta cttaagttt gttgggtat agttgttaa attcttggt aaaacgatg atattattt aaacatcac tccaactat aatacctat ttcacattt ttttcatta gtttgtagg taattcgtt aaatttata caaaattgt gcagtagag taatttatt atacatatt ataatattg ttgttgtgt tttgtgcaa tcaagggtg gaaagccag ctggtgttt caagtactt aaagtgtgg atgctgcaa agatcccta tgttttatg catttaagt cagaaggca aatgtggtc caagtcata tttttcaca tttgctaga ttggaagcg gaaggaaat atagtacaa cctattttc ttacagttt atgactatg tgatttatt tgatgacaa ggtagccat ggcagaaag taacatcag tattgtaag aggtgttgt gcatttttg cgcgagttt gtttcaact gccgaagtt tacatattt gggaacgaa gtggagatt tttatctcc ttgggcatg ttctgtacc tgaaaaaat atatgaaga aggaactaa tatttttat aataacaga atctcatct actagtgta acaaattat catcttagg tgtagtctt atagttata ttttttatg ccgttattg gatttacct aaaaccttc cattctttg tacaacagt aaatttccg cagcatttt ccttacact cattgtagt tcaacaggt aacaaccca aatttaact gtagctcac tattattta gcacttctt gtatttatt attttttta atgtctaat aaaacttta ttaaaaga