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Gene information
General information
TC001146 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11560510..11582449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001146
    iBeetle screen iB_03147

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001146 RA
      MESAVMTPG NTRLKVRGK NKSLTRLLN RNHSLSNKN 
NRNARPNSL RSSSNQRHL NHFPKSVRC NQIDNAAFV 
NNINNGNDT EERKIIFHG NFTREVYNA ICLRLGHTL 
YIPEDSVQS VFNDMQLYP SKDQVSEML KCARQCGRR 
NGASKYLTF GEFCVFVRE MQNQNPKQN RKIQSCKSN 
NKCGNNCEV FLGGSCNPT TWRADTAIP ELQKHGITF 
YNPQVSMWV PELVAQEHD AKQAASVLL YVVDNQTRS 
TVGMIEVAY LVASGRCVV IVAHSYRPG QSIMGETIT 
QREYRDLVE GQTTLLTLV RSKGIKVHQ TLPSALQCT 
AKILRNVSN DMTPEEQLT MKLRKLREI FDSCGGPNG 
EIDSLKFMK AFQQLTQRE LTNNELYKY FTPNNKSIT 
FEKFCMLMA ELTSDNCDT STTNGWVSQ PFQRQCSTN 
NNTCNVENS VLNGTIDEP FTNTSFKNK YDVFLGGTH 
NSLSNWRED VAIPTLKKY NIRYNPVST NGYEVLDNK 
SVSDEDVLE WKQMMDGAD VLLFVITED TRSLTTMIL 
AAHYIAIGK NLVLCVQHL PEENCVVGN ESLSEQAIK 
DYNRGRVYV TDMAKRKQI PVFEDIAEA VQCVVNMIQ 
SR
    CDS sequence
    • >TC001146 RA
      atggaaagt gcagttatg actccggga aatactcga 
ttgaaagta cgcgggaaa aataaatcg ttaacgcgg 
ctcctgaac cgtaatcac agcttgagc aataaaaac 
aatcggaat gctagaccc aattcgttg aggagcagc 
tcgaaccag agacattta aaccatttt ccgaagagt 
gtacgctgt aatcagatc gataacgct gccttcgta 
aacaatatc aacaatgga aatgatact gaagagagg 
aaaattatt ttccatgga aattttaca agagaggtt 
tacaatgca atatgcctt cggttagga cacaccctt 
tatattcct gaagattcc gtgcaatct gtttttaac 
gatatgcag ctttatcca tcgaaagat caagtgtca 
gaaatgttg aagtgtgcg agacagtgc ggtcgacga 
aatggggca tctaagtat ctcaccttt ggcgagttt 
tgtgtcttt gttcgagaa atgcaaaat cagaatccg 
aagcagaac cgcaaaata cagtcatgt aaatcgaac 
aacaaatgt ggaaataat tgtgaggta ttcttagga 
gggtcatgc aatcccaca acatggaga gccgatacg 
gcaattcca gagctccaa aaacacggt attacgttt 
tataatcca caagtttcg atgtgggtg cctgaactg 
gtggcccag gagcacgat gctaaacag gccgcctca 
gttttgctc tatgtggtg gacaaccaa actagaagc 
actgttggc atgattgaa gtcgcgtat ttggtggct 
tcagggcgg tgtgttgtc attgttgca cactcgtac 
agacctggg caaagtatc atgggggag acaatcacg 
cagagggag tatagagat ttggtagag ggccagaca 
acgttatta acgctagtt aggagtaaa gggattaaa 
gtccaccaa actttgccc tcagctttg caatgcact 
gcgaagatt ttaagaaat gttagcaac gatatgaca 
cccgaagaa cagctaact atgaaactg agaaaattg 
cgggaaatt tttgactcc tgtggaggt ccaaacggt 
gaaatagat tcgttaaaa tttatgaaa gccttccag 
caattaact cagcgtgaa ctaacgaat aacgaactt 
tacaagtat tttactccc aacaacaaa agcatcaca 
tttgagaaa ttctgcatg ttgatggcg gaactgacc 
agcgacaac tgtgatacg agcacgaca aatgggtgg 
gttagtcag ccttttcag cggcaatgt tcgacaaat 
aacaacact tgtaacgtg gaaaattct gtactaaat 
ggtacaata gacgaaccc ttcaccaac acgtcattt 
aaaaataag tatgatgtg tttcttggt ggaacgcat 
aattcacta agcaactgg cgagaagat gtagcaata 
ccaacgttg aaaaaatac aatataagg tataatcca 
gtttctacg aacggatat gaagtatta gataataag 
agtgtgagt gatgaagac gtcttggag tggaaacag 
atgatggat ggtgccgat gtgttattg tttgtcata 
acggaggat acgaggtca ttgacaact atgatcttg 
gccgctcat tacattgca ataggaaaa aaccttgtc 
ttgtgtgtt cagcacttg ccagaagag aactgtgtt 
gttggcaat gaatctctg tcggagcag gcgataaaa 
gactacaat cgcgggagg gtgtacgta acagatatg 
gcgaaacgt aaacaaatt ccagtattt gaagacatc 
gcagaagct gtgcaatgt gtagtcaac atgatacag 
agcagatag
    mRNA sequence
    • >TC001146 RA
      tgtcgctgg agagattag ttccaatcc acgttccga 
acccaatac attgtccta ctgtctgac ctcgcactt 
cttccaata gttgcgtag gaggactta aacgagata 
aataaacat tcagtgact tttgcttgt tccggtgat 
ttatttgcg caaagctgg cggcaccaa acgaagacg 
accaacaaa gtgtgttcc tgtatgtga gtgagtttg 
cgttgcgtc tctgcgtat ctgttcgtt catcggttc 
attttttac aagaatggc acgtgccac cgaatacat 
cacaagcca aataaacaa acatgtcga aagtgcaaa 
caaatatta gtgccatac accatgcaa agttcctgc 
acgaaatgt tggtaatca caccggtgc cgatatttt 
ctctatatt atttttaaa cgtacgttg gtgtctatc 
ctaaattta agtcgtaca tgcgaaacg tggagaaat 
caattttcc aaatgtcac gactctcga ggacaccac 
attgttagt ttgccgtta gttttggaa aatttccgc 
ggaaaaatg gaaagtgca gttatgact ccgggaaat 
actcgattg aaagtacgc gggaaaaat aaatcgtta 
acgcggctc ctgaaccgt aatcacagc ttgagcaat 
aaaaacaat cggaatgct agacccaat tcgttgagg 
agcagctcg aaccagaga catttaaac cattttccg 
aagagtgta cgctgtaat cagatcgat aacgctgcc 
ttcgtaaac aatatcaac aatggaaat gatactgaa 
gagaggaaa attattttc catggaaat tttacaaga 
gaggtttac aatgcaata tgccttcgg ttaggacac 
accctttat attcctgaa gattccgtg caatctgtt 
tttaacgat atgcagctt tatccatcg aaagatcaa 
gtgtcagaa atgttgaag tgtgcgaga cagtgcggt 
cgacgaaat ggggcatct aagtatctc acctttggc 
gagttttgt gtctttgtt cgagaaatg caaaatcag 
aatccgaag cagaaccgc aaaatacag tcatgtaaa 
tcgaacaac aaatgtgga aataattgt gaggtattc 
ttaggaggg tcatgcaat cccacaaca tggagagcc 
gatacggca attccagag ctccaaaaa cacggtatt 
acgttttat aatccacaa gtttcgatg tgggtgcct 
gaactggtg gcccaggag cacgatgct aaacaggcc 
gcctcagtt ttgctctat gtggtggac aaccaaact 
agaagcact gttggcatg attgaagtc gcgtatttg 
gtggcttca gggcggtgt gttgtcatt gttgcacac 
tcgtacaga cctgggcaa agtatcatg ggggagaca 
atcacgcag agggagtat agagatttg gtagagggc 
cagacaacg ttattaacg ctagttagg agtaaaggg 
attaaagtc caccaaact ttgccctca gctttgcaa 
tgcactgcg aagatttta agaaatgtt agcaacgat 
atgacaccc gaagaacag ctaactatg aaactgaga 
aaattgcgg gaaattttt gactcctgt ggaggtcca 
aacggtgaa atagattcg ttaaaattt atgaaagcc 
ttccagcaa ttaactcag cgtgaacta acgaataac 
gaactttac aagtatttt actcccaac aacaaaagc 
atcacattt gagaaattc tgcatgttg atggcggaa 
ctgaccagc gacaactgt gatacgagc acgacaaat 
gggtgggtt agtcagcct tttcagcgg caatgttcg 
acaaataac aacacttgt aacgtggaa aattctgta 
ctaaatggt acaatagac gaacccttc accaacacg 
tcatttaaa aataagtat gatgtgttt cttggtgga 
acgcataat tcactaagc aactggcga gaagatgta 
gcaatacca acgttgaaa aaatacaat ataaggtat 
aatccagtt tctacgaac ggatatgaa gtattagat 
aataagagt gtgagtgat gaagacgtc ttggagtgg 
aaacagatg atggatggt gccgatgtg ttattgttt 
gtcataacg gaggatacg aggtcattg acaactatg 
atcttggcc gctcattac attgcaata ggaaaaaac 
cttgtcttg tgtgttcag cacttgcca gaagagaac 
tgtgttgtt ggcaatgaa tctctgtcg gagcaggcg 
ataaaagac tacaatcgc gggagggtg tacgtaaca 
gatatggcg aaacgtaaa caaattcca gtatttgaa 
gacatcgca gaagctgtg caatgtgta gtcaacatg 
atacagagc agatagtca gatacctca cattttgtt 
ttataaatg aattggaat tttcatttc atgacacag 
gaaaaacat atttgctct cttgaacgg ttttgtaag 
gcagctctc ttcttgtaa agcagctcg tttcaggtg 
aaatggaat tttcgataa atacgcttt tatattttt 
attatttgt tattaatgt tttaatgta actctaatt 
tcattgtta ttgaacact tattggtgc agtttttta 
tttgtttga tcattgtaa cggaaatct aacttgtgg 
atgtttccc actaagata atgttagat gtaaagtgg 
tataatttt tattaactg aaattcttg tttaacagc 
tatactttg atatgtaca gtgtaaaaa gtgatgaag 
gtagctaat gttggtaaa gacaagttc ctctctcaa 
atgtaaata cggctgcta cttaagttt gttgggtat 
agttgttaa attcttggt aaaacgatg atattattt 
aaacatcac tccaactat aatacctat ttcacattt 
ttttcatta gtttgtagg taattcgtt aaatttata 
caaaattgt gcagtagag taatttatt atacatatt 
ataatattg ttgttgtgt tttgtgcaa tcaagggtg 
gaaagccag ctggtgttt caagtactt aaagtgtgg 
atgctgcaa agatcccta tgttttatg catttaagt 
cagaaggca aatgtggtc caagtcata tttttcaca 
tttgctaga ttggaagcg gaaggaaat atagtacaa 
cctattttc ttacagttt atgactatg tgatttatt 
tgatgacaa ggtagccat ggcagaaag taacatcag 
tattgtaag aggtgttgt gcatttttg cgcgagttt 
gtttcaact gccgaagtt tacatattt gggaacgaa 
gtggagatt tttatctcc ttgggcatg ttctgtacc 
tgaaaaaat atatgaaga aggaactaa tatttttat 
aataacaga atctcatct actagtgta acaaattat 
catcttagg tgtagtctt atagttata ttttttatg 
ccgttattg gatttacct aaaaccttc cattctttg 
tacaacagt aaatttccg cagcatttt ccttacact 
cattgtagt tcaacaggt aacaaccca aatttaact 
gtagctcac tattattta gcacttctt gtatttatt 
attttttta atgtctaat aaaacttta ttaaaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY