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Gene information
General information
TC001146 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6053967..6075906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001146
    iBeetle screen iB_03147
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001146 RA
      MESAVMTPG NTRLKVRGK NKSLTRLLN RNHSLSNKN 
      NRNARPNSL RSSSNQRHL NHFPKSVRC NQIDNAAFV 
      NNINNGNDT EERKIIFHG NFTREVYNA ICLRLGHTL 
      YIPEDSVQS VFNDMQLYP SKDQVSEML KCARQCGRR 
      NGASKYLTF GEFCVFVRE MQNQNPKQN RKIQSCKSN 
      NKCGNNCEV FLGGSCNPT TWRADTAIP ELQKHGITF 
      YNPQVSMWV PELVAQEHD AKQAASVLL YVVDNQTRS 
      TVGMIEVAY LVASGRCVV IVAHSYRPG QSIMGETIT 
      QREYRDLVE GQTTLLTLV RSKGIKVHQ TLPSALQCT 
      AKILRNVSN DMTPEEQLT MKLRKLREI FDSCGGPNG 
      EIDSLKFMK AFQQLTQRE LTNNELYKY FTPNNKSIT 
      FEKFCMLMA ELTSDNCDT STTNGWVSQ PFQRQCSTN 
      NNTCNVENS VLNGTIDEP FTNTSFKNK YDVFLGGTH 
      NSLSNWRED VAIPTLKKY NIRYNPVST NGYEVLDNK 
      SVSDEDVLE WKQMMDGAD VLLFVITED TRSLTTMIL 
      AAHYIAIGK NLVLCVQHL PEENCVVGN ESLSEQAIK 
      DYNRGRVYV TDMAKRKQI PVFEDIAEA VQCVVNMIQ 
      SR
    CDS sequence
    • >TC001146 RA
      atggaaagt gcagttatg actccggga aatactcga 
      ttgaaagta cgcgggaaa aataaatcg ttaacgcgg 
      ctcctgaac cgtaatcac agcttgagc aataaaaac 
      aatcggaat gctagaccc aattcgttg aggagcagc 
      tcgaaccag agacattta aaccatttt ccgaagagt 
      gtacgctgt aatcagatc gataacgct gccttcgta 
      aacaatatc aacaatgga aatgatact gaagagagg 
      aaaattatt ttccatgga aattttaca agagaggtt 
      tacaatgca atatgcctt cggttagga cacaccctt 
      tatattcct gaagattcc gtgcaatct gtttttaac 
      gatatgcag ctttatcca tcgaaagat caagtgtca 
      gaaatgttg aagtgtgcg agacagtgc ggtcgacga 
      aatggggca tctaagtat ctcaccttt ggcgagttt 
      tgtgtcttt gttcgagaa atgcaaaat cagaatccg 
      aagcagaac cgcaaaata cagtcatgt aaatcgaac 
      aacaaatgt ggaaataat tgtgaggta ttcttagga 
      gggtcatgc aatcccaca acatggaga gccgatacg 
      gcaattcca gagctccaa aaacacggt attacgttt 
      tataatcca caagtttcg atgtgggtg cctgaactg 
      gtggcccag gagcacgat gctaaacag gccgcctca 
      gttttgctc tatgtggtg gacaaccaa actagaagc 
      actgttggc atgattgaa gtcgcgtat ttggtggct 
      tcagggcgg tgtgttgtc attgttgca cactcgtac 
      agacctggg caaagtatc atgggggag acaatcacg 
      cagagggag tatagagat ttggtagag ggccagaca 
      acgttatta acgctagtt aggagtaaa gggattaaa 
      gtccaccaa actttgccc tcagctttg caatgcact 
      gcgaagatt ttaagaaat gttagcaac gatatgaca 
      cccgaagaa cagctaact atgaaactg agaaaattg 
      cgggaaatt tttgactcc tgtggaggt ccaaacggt 
      gaaatagat tcgttaaaa tttatgaaa gccttccag 
      caattaact cagcgtgaa ctaacgaat aacgaactt 
      tacaagtat tttactccc aacaacaaa agcatcaca 
      tttgagaaa ttctgcatg ttgatggcg gaactgacc 
      agcgacaac tgtgatacg agcacgaca aatgggtgg 
      gttagtcag ccttttcag cggcaatgt tcgacaaat 
      aacaacact tgtaacgtg gaaaattct gtactaaat 
      ggtacaata gacgaaccc ttcaccaac acgtcattt 
      aaaaataag tatgatgtg tttcttggt ggaacgcat 
      aattcacta agcaactgg cgagaagat gtagcaata 
      ccaacgttg aaaaaatac aatataagg tataatcca 
      gtttctacg aacggatat gaagtatta gataataag 
      agtgtgagt gatgaagac gtcttggag tggaaacag 
      atgatggat ggtgccgat gtgttattg tttgtcata 
      acggaggat acgaggtca ttgacaact atgatcttg 
      gccgctcat tacattgca ataggaaaa aaccttgtc 
      ttgtgtgtt cagcacttg ccagaagag aactgtgtt 
      gttggcaat gaatctctg tcggagcag gcgataaaa 
      gactacaat cgcgggagg gtgtacgta acagatatg 
      gcgaaacgt aaacaaatt ccagtattt gaagacatc 
      gcagaagct gtgcaatgt gtagtcaac atgatacag 
      agcagatag
    mRNA sequence
    • >TC001146 RA
      tgtcgctgg agagattag ttccaatcc acgttccga 
      acccaatac attgtccta ctgtctgac ctcgcactt 
      cttccaata gttgcgtag gaggactta aacgagata 
      aataaacat tcagtgact tttgcttgt tccggtgat 
      ttatttgcg caaagctgg cggcaccaa acgaagacg 
      accaacaaa gtgtgttcc tgtatgtga gtgagtttg 
      cgttgcgtc tctgcgtat ctgttcgtt catcggttc 
      attttttac aagaatggc acgtgccac cgaatacat 
      cacaagcca aataaacaa acatgtcga aagtgcaaa 
      caaatatta gtgccatac accatgcaa agttcctgc 
      acgaaatgt tggtaatca caccggtgc cgatatttt 
      ctctatatt atttttaaa cgtacgttg gtgtctatc 
      ctaaattta agtcgtaca tgcgaaacg tggagaaat 
      caattttcc aaatgtcac gactctcga ggacaccac 
      attgttagt ttgccgtta gttttggaa aatttccgc 
      ggaaaaatg gaaagtgca gttatgact ccgggaaat 
      actcgattg aaagtacgc gggaaaaat aaatcgtta 
      acgcggctc ctgaaccgt aatcacagc ttgagcaat 
      aaaaacaat cggaatgct agacccaat tcgttgagg 
      agcagctcg aaccagaga catttaaac cattttccg 
      aagagtgta cgctgtaat cagatcgat aacgctgcc 
      ttcgtaaac aatatcaac aatggaaat gatactgaa 
      gagaggaaa attattttc catggaaat tttacaaga 
      gaggtttac aatgcaata tgccttcgg ttaggacac 
      accctttat attcctgaa gattccgtg caatctgtt 
      tttaacgat atgcagctt tatccatcg aaagatcaa 
      gtgtcagaa atgttgaag tgtgcgaga cagtgcggt 
      cgacgaaat ggggcatct aagtatctc acctttggc 
      gagttttgt gtctttgtt cgagaaatg caaaatcag 
      aatccgaag cagaaccgc aaaatacag tcatgtaaa 
      tcgaacaac aaatgtgga aataattgt gaggtattc 
      ttaggaggg tcatgcaat cccacaaca tggagagcc 
      gatacggca attccagag ctccaaaaa cacggtatt 
      acgttttat aatccacaa gtttcgatg tgggtgcct 
      gaactggtg gcccaggag cacgatgct aaacaggcc 
      gcctcagtt ttgctctat gtggtggac aaccaaact 
      agaagcact gttggcatg attgaagtc gcgtatttg 
      gtggcttca gggcggtgt gttgtcatt gttgcacac 
      tcgtacaga cctgggcaa agtatcatg ggggagaca 
      atcacgcag agggagtat agagatttg gtagagggc 
      cagacaacg ttattaacg ctagttagg agtaaaggg 
      attaaagtc caccaaact ttgccctca gctttgcaa 
      tgcactgcg aagatttta agaaatgtt agcaacgat 
      atgacaccc gaagaacag ctaactatg aaactgaga 
      aaattgcgg gaaattttt gactcctgt ggaggtcca 
      aacggtgaa atagattcg ttaaaattt atgaaagcc 
      ttccagcaa ttaactcag cgtgaacta acgaataac 
      gaactttac aagtatttt actcccaac aacaaaagc 
      atcacattt gagaaattc tgcatgttg atggcggaa 
      ctgaccagc gacaactgt gatacgagc acgacaaat 
      gggtgggtt agtcagcct tttcagcgg caatgttcg 
      acaaataac aacacttgt aacgtggaa aattctgta 
      ctaaatggt acaatagac gaacccttc accaacacg 
      tcatttaaa aataagtat gatgtgttt cttggtgga 
      acgcataat tcactaagc aactggcga gaagatgta 
      gcaatacca acgttgaaa aaatacaat ataaggtat 
      aatccagtt tctacgaac ggatatgaa gtattagat 
      aataagagt gtgagtgat gaagacgtc ttggagtgg 
      aaacagatg atggatggt gccgatgtg ttattgttt 
      gtcataacg gaggatacg aggtcattg acaactatg 
      atcttggcc gctcattac attgcaata ggaaaaaac 
      cttgtcttg tgtgttcag cacttgcca gaagagaac 
      tgtgttgtt ggcaatgaa tctctgtcg gagcaggcg 
      ataaaagac tacaatcgc gggagggtg tacgtaaca 
      gatatggcg aaacgtaaa caaattcca gtatttgaa 
      gacatcgca gaagctgtg caatgtgta gtcaacatg 
      atacagagc agatagtca gatacctca cattttgtt 
      ttataaatg aattggaat tttcatttc atgacacag 
      gaaaaacat atttgctct cttgaacgg ttttgtaag 
      gcagctctc ttcttgtaa agcagctcg tttcaggtg 
      aaatggaat tttcgataa atacgcttt tatattttt 
      attatttgt tattaatgt tttaatgta actctaatt 
      tcattgtta ttgaacact tattggtgc agtttttta 
      tttgtttga tcattgtaa cggaaatct aacttgtgg 
      atgtttccc actaagata atgttagat gtaaagtgg 
      tataatttt tattaactg aaattcttg tttaacagc 
      tatactttg atatgtaca gtgtaaaaa gtgatgaag 
      gtagctaat gttggtaaa gacaagttc ctctctcaa 
      atgtaaata cggctgcta cttaagttt gttgggtat 
      agttgttaa attcttggt aaaacgatg atattattt 
      aaacatcac tccaactat aatacctat ttcacattt 
      ttttcatta gtttgtagg taattcgtt aaatttata 
      caaaattgt gcagtagag taatttatt atacatatt 
      ataatattg ttgttgtgt tttgtgcaa tcaagggtg 
      gaaagccag ctggtgttt caagtactt aaagtgtgg 
      atgctgcaa agatcccta tgttttatg catttaagt 
      cagaaggca aatgtggtc caagtcata tttttcaca 
      tttgctaga ttggaagcg gaaggaaat atagtacaa 
      cctattttc ttacagttt atgactatg tgatttatt 
      tgatgacaa ggtagccat ggcagaaag taacatcag 
      tattgtaag aggtgttgt gcatttttg cgcgagttt 
      gtttcaact gccgaagtt tacatattt gggaacgaa 
      gtggagatt tttatctcc ttgggcatg ttctgtacc 
      tgaaaaaat atatgaaga aggaactaa tatttttat 
      aataacaga atctcatct actagtgta acaaattat 
      catcttagg tgtagtctt atagttata ttttttatg 
      ccgttattg gatttacct aaaaccttc cattctttg 
      tacaacagt aaatttccg cagcatttt ccttacact 
      cattgtagt tcaacaggt aacaaccca aatttaact 
      gtagctcac tattattta gcacttctt gtatttatt 
      attttttta atgtctaat aaaacttta ttaaaaga