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Gene information
General information
TC001129 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11411812..11427114
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001129
    iBeetle screen iB_03143

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001129 RA
      MDQKTNKYV RTFSGHFVK VRFKQTNLL FVCVIVVCV 
      INSVQCNRP PRFLINGQT EIVLRLKEG KETPVGTLI 
      YKLRGTDPD GDPLSFGVR SQSGSDVIR VENISPTEA 
      DVYLNKELD RETRDEYPL VLTLTDGRL GHGNYVTQS 
      LLLLVEDVN DNTPIFTSH QSSITLRED AAPGVIATL 
      EATDLDEGA YGQVVYQLQ EVDTKESLF SVSTAGGKA 
      IVRLVGSLD YEKQTLHQL KVLAVDRAK QGRVNTGTA 
      ALLVKVEDV EDQPPEFVR VTSVARIAE NAPIGTSVL 
      QVTAIDGDR GVNNPILYS LSSNSIDRI TDVFSLEET 
      TGTLLTAKT LDRESLSLS SGTYILQIT ATEYGGKIK 
      PPPSVTTEV TVIITDIND ETPKFRSGY YECEIAENA 
      PVNTPLTFL GSAMPEVYD HDQGINGTF QLYVKGASD 
      VFEITPTKA INEATFMIR VKNSTFLDY EKIKTINFT 
      LVAKEIVKH NPKFNEVPV VAHILDRND NYPEFTKNV 
      YEVFVPENC DIGATVAWV QALDDDSGV FGTMGIRYT 
      HLSGSIQHM LNLHPTTGI ISVKTAGGP NWDREQISR 
      HYLTVEARD DLGQGNRIT VQLIINIED VNDNPPIFT 
      QNRYEARLL ENKLDFENP LKVEARDAD LNGTKNNEI 
      EYSLFGDLS HNFTIEPST GIIKPRTSI DFEALEGSL 
      KDSIRQLHL TVRARDWGT PSLFSDVPL YIYVQDVND 
      HAPLFEYTF YNKSVPEDI SGGTSILQV RAKDFDGSS 
      PNNRIVYRI QNGASDKFI IAPESGIIS VARGASLDP 
      DLTQPRTLH YSLNVVALD GAPGENQLQ AGVTVNVTI 
      LDINNKNPV FYEPGSVTM RENTAVGSV IAKLKAKDL 
      DKTAQLVYT INPKFCEAK NERGILLKL SDFNCAETF 
      HLHPMSGVL TIAQQLDRE MVEKVQMGL MVEDTASET 
      GPQISTTTI TINIEDIND NSPKFRQPY YKFSITENS 
      KNGVTIGNV LAEDADKNK TITYTLDGR FEITELIYL 
      DSNSGDLVV ANKIDHEIY DWLNLTIKA TDSGIPARF 
      SRVEIFIQI LDENDNNPF FLGEPQSLL ISEDTPVGR 
      QVAIIEAKD ADSGEFGKI TYLLDRISS EGKFSIDAD 
      SGILKVSDK LDREEKHSY LLVIEAWDN YQYGFNSGE 
      SRNAFKHIN VTILDVNDN HPQLHVPPY CVNITEFHE 
      PGQAITTFH ASDADDPNT SNGQVIIDI SDGNQKNFF 
      ALSQISEWS AELRTTTSL RGRHGNYTL IIRAQDLGT 
      PSHLIEEPL HICVTDFND HPPTFVTPP HNSTLRVPE 
      NATIGSALV KIIATDEDV GSNGIVRYR LKADPAGHW 
      KSFNLQPVS GILELRLPL DRKKQKVYD IRIEAYDLG 
      VPSLSSDLD LTVYVSNIN DYQPQFMGD EFTVNFTEN 
      KPSEVERQK LPQTVDRDE LEFDGPFAP ICYFIVSRP 
      LDREEKDTH LLLVKATED CTKPPRNES FFDSSDDTQ 
      LKVIVKVLD VNDNAPQFI HRVFTGGVS TATSFGTTF 
      MEVKAEDAD LDDNARMSY FLIGKVQMT LTEGLEDLK 
      RDPFLVDRD TGAVQLNFD PQEGMKGYF DFMVLVNDT 
      GGLQDMARV FIYLLRADQ RVRFVLRQQ PPELRNKIE 
      AFREILGNV TGAIVNVDE FRVHANHDG SVDKTRTDL 
      YLHLVDRRD NSIIEVDEV LRLVDQNTE KLDELFKEF 
      NVLDTQPGG PLALKAAKS ANTTFWLTA ATLFLLLLL 
      LLCLALCIN QRQTYHRKL KAATATAYV TTDSDIDGR 
      GLSALSGRV PNTNKHSME GSNPIWMQA YENEWYKNA 
      DEFSQNSEH DSLDENVVS CGDLPQCNN LQHTNGHIR 
      AQNVYQTLP PALPRRKLE TTEL
    CDS sequence
    • >TC001129 RA
      atggatcag aagacgaat aaatatgtg aggacattt 
      agtggacac tttgtcaaa gtgcggttc aaacaaaca 
      aatctttta tttgtgtgt gtgattgtt gtgtgtgtt 
      ataaattca gtgcaatgt aatagacca cctaggttc 
      ttaattaac ggccaaacc gaaattgtt ctaagactg 
      aaagaaggc aaagaaacg ccagtaggt accttaatt 
      tataaactg cgtggaacc gatcctgat ggtgacccc 
      cttagtttt ggagtcaga tctcaatca ggaagtgat 
      gttatccgt gttgaaaat atatcgcca accgaagca 
      gacgtctac ctcaataaa gaactagac agagaaaca 
      cgcgatgaa tatccatta gtgttaact ctaactgac 
      ggtcggttg ggacatggc aattacgtc actcaaagt 
      cttttatta ctagtcgaa gatgtcaac gacaataca 
      ccgatattc acaagtcat caatcatcg attacttta 
      agagaggat gcagcaccg ggggttatc gcgactttg 
      gaagccact gatttagat gaaggtgct tacggtcaa 
      gttgtttat cagctgcaa gaagttgat acaaaagag 
      tctttattt agtgttagt acggccggg gggaaagca 
      atcgtgcgc ttagtaggt tctttagac tacgagaaa 
      caaactttg caccagtta aaagtcttg gcagttgac 
      cgcgcaaaa caaggaaga gttaacacc ggaactgcc 
      gctctttta gtaaaagtt gaagatgtg gaagaccaa 
      ccgcctgaa tttgttaga gtcacttca gtggcgcga 
      atcgctgaa aatgcgcca attgggact tcagtcctt 
      caagtcact gctattgac ggagaccgt ggtgttaac 
      aatccgatt ctatattca ctgtcgtct aatagtatt 
      gacagaatc acagatgtg tttagttta gaagaaacc 
      accggaacg ctcctcact gccaaaacc ttagaccga 
      gaatctctt tctctaagt tccggtact tacatcctc 
      caaataacg gcgactgaa tacggcggt aaaatcaaa 
      cctccacca agtgttaca accgaagtc acagtaatt 
      atcacggat atcaatgac gaaacgccc aaatttagg 
      agcggttat tatgaatgc gaaatagcg gaaaatgca 
      ccagtcaac acaccattg accttcctc ggctccgcc 
      atgcctgaa gtctacgac cacgaccag ggaatcaac 
      ggtactttc caactatac gtgaaaggg gcaagcgat 
      gtgtttgag ataacaccc accaaagca ataaacgaa 
      gctactttc atgatcaga gtgaaaaat tcaacgttt 
      ctagattac gagaagatt aaaactatc aatttcact 
      ctagtcgcc aaagaaata gtgaaacac aatccaaaa 
      tttaacgag gtgccagtc gtcgcccat attttagac 
      cggaatgac aattacccg gaattcaca aaaaatgtt 
      tacgaggtt tttgtaccg gaaaattgc gatattggc 
      gctacggtg gcgtgggtc caagcgctg gacgatgac 
      tcgggggtt tttggcacc atgggcatt cgctacacc 
      cacttgtcc ggcagcatt cagcacatg ttaaacttg 
      cacccaact actggaatt atctcggtt aagacagcc 
      gggggacca aactgggac cgcgagcaa atctcgcgg 
      cactactta acagtggaa gcaagggac gatttaggg 
      caaggaaat cgtatcacg gttcagctt attatcaat 
      attgaagat gttaacgat aatcccccg attttcact 
      caaaatcgc tacgaggcg cgcttatta gagaataag 
      cttgatttt gaaaatccc ctaaaggtc gaagctcgc 
      gatgctgat ttgaacggt acaaaaaac aacgaaatt 
      gaatacagc ctgttcgga gacttgagc cacaatttc 
      acgatcgag ccctcgacc gggatcatc aaaccgcgg 
      acaagtatt gactttgag gccctcgaa gggtccttg 
      aaggacagc attcgccag ctccactta acagtccga 
      gctagggat tggggcact cctagttta tttagcgat 
      gttccattg tacatttac gttcaagac gtaaacgat 
      cacgcccct ctctttgaa tacacgttc tacaataaa 
      tccgtcccg gaagacatc tctggcggc acctctatt 
      ctccaagtc agagccaag gattttgac ggctcttcg 
      cccaacaat cgaatcgta tacagaata cagaatggg 
      gcatctgat aaatttata atagccccc gagtccgga 
      attatctca gtggcgaga ggagcttca ctcgatccc 
      gacttgacc cagcccaga acattacat tactcttta 
      aatgttgtg gctctggat ggtgcgccc ggggaaaat 
      caattgcaa gccggggtt acggttaat gtcaccata 
      ttggacatt aataataaa aatccggtt ttttacgaa 
      ccgggaagc gtaacaatg cgagaaaac acagcggtc 
      gggagtgtt atagcaaaa ttaaaagcc aaagacttg 
      gacaaaact gcccaatta gtttatacc atcaacccc 
      aaattttgc gaagctaag aatgaacga ggaatttta 
      ctcaaactg agcgatttt aattgcgct gaaacgttc 
      catttgcac ccgatgagt ggtgtcctg acaatcgcc 
      caacaactg gaccgggaa atggtcgaa aaggtgcaa 
      atgggactc atggtcgaa gatacggcc tccgaaacg 
      gggccacaa atttcaacc actacaatt acgataaat 
      atcgaggac ataaacgac aacagcccg aagttccgc 
      cagccctat tacaagttt tccattacc gaaaacagc 
      aaaaacggc gtaacgatt gggaatgtc ttggccgag 
      gacgccgac aagaataaa acgataact tacacactg 
      gacggccgt ttcgaaata acggaactt atttatttg 
      gatagcaat tccggggat ttggtggtc gcaaataaa 
      atcgatcac gaaatttat gactggtta aatctgacg 
      attaaagcg acagactcg ggaattccg gcgcgcttt 
      tcccgagtc gagattttc attcaaatc ttggatgaa 
      aacgacaac aacccgttt tttctgggc gaaccacaa 
      tcgctgcta atatccgaa gacactccc gtgggaaga 
      caagtcgca ataatcgaa gccaaggat gctgattct 
      ggagagttc gggaagatt acctattta ctagaccgg 
      atttcatcc gaaggcaaa ttcagcata gacgcggat 
      tcgggaatt ttgaaagtg tcggataaa ttggatcgg 
      gaggagaaa cacagttac ctgctcgta attgaagct 
      tgggataat taccaatac gggttcaac agtggagaa 
      agcagaaac gcattcaaa catatcaat gtaactatt 
      ctagatgtt aacgataac catccgcag cttcatgtt 
      ccaccttat tgcgtcaat atcacagaa tttcatgaa 
      ccaggacaa gccataacc acgtttcac gcttcagat 
      gccgatgat cctaatact tcaaatgga caggtcata 
      attgacata tctgacggc aatcagaag aactttttt 
      gccttgtcg caaataagc gaatggtcg gccgagcta 
      cgaactacc acttcactg agaggcaga cacggaaac 
      tacacatta attataaga gcgcaagac ttggggaca 
      ccaagtcac ttaattgaa gaacctctc catatctgt 
      gtaactgac tttaatgac catcctccc acgtttgtg 
      actcctccg cacaactca actctgcga gtgccagaa 
      aatgcaacg attggaagc gccctagtt aaaattata 
      gccacggac gaagacgtc ggctcgaac ggaattgtg 
      cggtatcgg ctcaaagct gatccagca ggacactgg 
      aaaagcttc aatttgcaa ccggtctca ggaatacta 
      gaactgcgg ctaccttta gaccgaaaa aaacagaaa 
      gtgtacgat attagaatc gaagcttac gacttggga 
      gtgccgtct ctcagctct gatctggat ctgacagtt 
      tacgtaagc aacatcaac gactaccaa ccccagttc 
      atgggtgat gaattcaca gtcaatttc actgaaaac 
      aaaccatcc gaagtggaa agacaaaaa cttccccaa 
      accgtcgat cgagatgag ctcgaattc gacgggccg 
      ttcgcacca atttgttat tttatagtg agtaggcct 
      ttagatcga gaagaaaaa gacacacat ttattgctc 
      gtcaaagca acagaagat tgcacaaaa ccgccacgc 
      aacgaaagc ttcttcgat tcctccgat gatacgcaa 
      ctcaaagtc atagtaaaa gtgttggat gtcaatgat 
      aatgcccca cagttcatc cacagggtt ttcaccggt 
      ggagtcagt acggcaacg agctttgga acaaccttt 
      atggaagtc aaggcggaa gatgccgac ttggacgac 
      aacgcccga atgtcctat ttcctgatc ggaaaagtg 
      caaatgacc ctgactgaa ggtttagaa gatttgaaa 
      agagaccct tttttggtg gatcgggac acaggagct 
      gtccaattg aatttcgat cctcaggaa ggaatgaag 
      ggttatttc gacttcatg gtcctagtg aacgacact 
      ggaggcctc caggacatg gctcgggtt ttcatttat 
      ttgcttagg gccgaccaa agagtccga tttgtctta 
      agacagcaa cctcccgaa ttgcgaaat aaaattgag 
      gcgtttcga gaaattttg ggtaacgtg accggagct 
      attgtgaac gttgacgaa tttcgagtt cacgcaaat 
      catgatggt agcgttgac aaaacccga accgatctg 
      tacctacac ttggttgat agacgcgat aattccata 
      atcgaagtt gacgaagtc ttgcgctta gttgaccaa 
      aataccgaa aaactcgat gaattattt aaagagttt 
      aacgtgttg gacacccaa ccgggagga ccgcttgcc 
      ttgaaagcg gcgaaatca gccaatacg acgttctgg 
      ttaactgct gcaacttta ttcctactg ctcctcctc 
      ttgttgtgc ctagctctt tgcataaac caacgacaa 
      acctaccac cggaaatta aaagcggcc acagcaacc 
      gcatatgta actacagat tccgacata gacggtcgg 
      ggtttaagc gctttgagt ggaagagta ccaaacaca 
      aataagcac agcatggag ggaagcaac cccatttgg 
      atgcaagct tatgagaac gaatggtac aaaaatgcg 
      gacgaattt agtcaaaat tcggaacac gattctctt 
      gatgaaaac gtggtgagc tgcggggat ctcccacag 
      tgcaacaat ttgcagcat accaatggt cacattcgt 
      gcccaaaat gtgtaccaa acgttacca ccagcattg 
      ccaagaaga aaactcgaa acaacggaa ctataa
    mRNA sequence
    • >TC001129 RA
      tgacgctta tttacttca cagtgcttt cagaataat 
      ttaccgtca tttacggat aagtgcagg aagatagat 
      agatacctg aaggagctt aggctcgaa gctcggaat 
      tgttttgtg tgtggtgcc tggagacaa gcttaatcc 
      tgtgaaagt gtacccgta aaaataagc tgaaaatca 
      gtcatagag aagagcctt aaccatgaa gaagacgca 
      cgccaagtc gtgcaacaa acacaggat ttatgcatc 
      gcaaatgtg ggctaagga agaaaaata gcgcacatc 
      accatcatg gatcagaag acgaataaa tatgtgagg 
      acatttagt ggacacttt gtcaaagtg cggttcaaa 
      caaacaaat cttttattt gtgtgtgtg attgttgtg 
      tgtgttata aattcagtg caatgtaat agaccacct 
      aggttctta attaacggc caaaccgaa attgttcta 
      agactgaaa gaaggcaaa gaaacgcca gtaggtacc 
      ttaatttat aaactgcgt ggaaccgat cctgatggt 
      gaccccctt agttttgga gtcagatct caatcagga 
      agtgatgtt atccgtgtt gaaaatata tcgccaacc 
      gaagcagac gtctacctc aataaagaa ctagacaga 
      gaaacacgc gatgaatat ccattagtg ttaactcta 
      actgacggt cggttggga catggcaat tacgtcact 
      caaagtctt ttattacta gtcgaagat gtcaacgac 
      aatacaccg atattcaca agtcatcaa tcatcgatt 
      actttaaga gaggatgca gcaccgggg gttatcgcg 
      actttggaa gccactgat ttagatgaa ggtgcttac 
      ggtcaagtt gtttatcag ctgcaagaa gttgataca 
      aaagagtct ttatttagt gttagtacg gccgggggg 
      aaagcaatc gtgcgctta gtaggttct ttagactac 
      gagaaacaa actttgcac cagttaaaa gtcttggca 
      gttgaccgc gcaaaacaa ggaagagtt aacaccgga 
      actgccgct cttttagta aaagttgaa gatgtggaa 
      gaccaaccg cctgaattt gttagagtc acttcagtg 
      gcgcgaatc gctgaaaat gcgccaatt gggacttca 
      gtccttcaa gtcactgct attgacgga gaccgtggt 
      gttaacaat ccgattcta tattcactg tcgtctaat 
      agtattgac agaatcaca gatgtgttt agtttagaa 
      gaaaccacc ggaacgctc ctcactgcc aaaacctta 
      gaccgagaa tctctttct ctaagttcc ggtacttac 
      atcctccaa ataacggcg actgaatac ggcggtaaa 
      atcaaacct ccaccaagt gttacaacc gaagtcaca 
      gtaattatc acggatatc aatgacgaa acgcccaaa 
      tttaggagc ggttattat gaatgcgaa atagcggaa 
      aatgcacca gtcaacaca ccattgacc ttcctcggc 
      tccgccatg cctgaagtc tacgaccac gaccaggga 
      atcaacggt actttccaa ctatacgtg aaaggggca 
      agcgatgtg tttgagata acacccacc aaagcaata 
      aacgaagct actttcatg atcagagtg aaaaattca 
      acgtttcta gattacgag aagattaaa actatcaat 
      ttcactcta gtcgccaaa gaaatagtg aaacacaat 
      ccaaaattt aacgaggtg ccagtcgtc gcccatatt 
      ttagaccgg aatgacaat tacccggaa ttcacaaaa 
      aatgtttac gaggttttt gtaccggaa aattgcgat 
      attggcgct acggtggcg tgggtccaa gcgctggac 
      gatgactcg ggggttttt ggcaccatg ggcattcgc 
      tacacccac ttgtccggc agcattcag cacatgtta 
      aacttgcac ccaactact ggaattatc tcggttaag 
      acagccggg ggaccaaac tgggaccgc gagcaaatc 
      tcgcggcac tacttaaca gtggaagca agggacgat 
      ttagggcaa ggaaatcgt atcacggtt cagcttatt 
      atcaatatt gaagatgtt aacgataat cccccgatt 
      ttcactcaa aatcgctac gaggcgcgc ttattagag 
      aataagctt gattttgaa aatccccta aaggtcgaa 
      gctcgcgat gctgatttg aacggtaca aaaaacaac 
      gaaattgaa tacagcctg ttcggagac ttgagccac 
      aatttcacg atcgagccc tcgaccggg atcatcaaa 
      ccgcggaca agtattgac tttgaggcc ctcgaaggg 
      tccttgaag gacagcatt cgccagctc cacttaaca 
      gtccgagct agggattgg ggcactcct agtttattt 
      agcgatgtt ccattgtac atttacgtt caagacgta 
      aacgatcac gcccctctc tttgaatac acgttctac 
      aataaatcc gtcccggaa gacatctct ggcggcacc 
      tctattctc caagtcaga gccaaggat tttgacggc 
      tcttcgccc aacaatcga atcgtatac agaatacag 
      aatggggca tctgataaa tttataata gcccccgag 
      tccggaatt atctcagtg gcgagagga gcttcactc 
      gatcccgac ttgacccag cccagaaca ttacattac 
      tctttaaat gttgtggct ctggatggt gcgcccggg 
      gaaaatcaa ttgcaagcc ggggttacg gttaatgtc 
      accatattg gacattaat aataaaaat ccggttttt 
      tacgaaccg ggaagcgta acaatgcga gaaaacaca 
      gcggtcggg agtgttata gcaaaatta aaagccaaa 
      gacttggac aaaactgcc caattagtt tataccatc 
      aaccccaaa ttttgcgaa gctaagaat gaacgagga 
      attttactc aaactgagc gattttaat tgcgctgaa 
      acgttccat ttgcacccg atgagtggt gtcctgaca 
      atcgcccaa caactggac cgggaaatg gtcgaaaag 
      gtgcaaatg ggactcatg gtcgaagat acggcctcc 
      gaaacgggg ccacaaatt tcaaccact acaattacg 
      ataaatatc gaggacata aacgacaac agcccgaag 
      ttccgccag ccctattac aagttttcc attaccgaa 
      aacagcaaa aacggcgta acgattggg aatgtcttg 
      gccgaggac gccgacaag aataaaacg ataacttac 
      acactggac ggccgtttc gaaataacg gaacttatt 
      tatttggat agcaattcc ggggatttg gtggtcgca 
      aataaaatc gatcacgaa atttatgac tggttaaat 
      ctgacgatt aaagcgaca gactcggga attccggcg 
      cgcttttcc cgagtcgag attttcatt caaatcttg 
      gatgaaaac gacaacaac ccgtttttt ctgggcgaa 
      ccacaatcg ctgctaata tccgaagac actcccgtg 
      ggaagacaa gtcgcaata atcgaagcc aaggatgct 
      gattctgga gagttcggg aagattacc tatttacta 
      gaccggatt tcatccgaa ggcaaattc agcatagac 
      gcggattcg ggaattttg aaagtgtcg gataaattg 
      gatcgggag gagaaacac agttacctg ctcgtaatt 
      gaagcttgg gataattac caatacggg ttcaacagt 
      ggagaaagc agaaacgca ttcaaacat atcaatgta 
      actattcta gatgttaac gataaccat ccgcagctt 
      catgttcca ccttattgc gtcaatatc acagaattt 
      catgaacca ggacaagcc ataaccacg tttcacgct 
      tcagatgcc gatgatcct aatacttca aatggacag 
      gtcataatt gacatatct gacggcaat cagaagaac 
      ttttttgcc ttgtcgcaa ataagcgaa tggtcggcc 
      gagctacga actaccact tcactgaga ggcagacac 
      ggaaactac acattaatt ataagagcg caagacttg 
      gggacacca agtcactta attgaagaa cctctccat 
      atctgtgta actgacttt aatgaccat cctcccacg 
      tttgtgact cctccgcac aactcaact ctgcgagtg 
      ccagaaaat gcaacgatt ggaagcgcc ctagttaaa 
      attatagcc acggacgaa gacgtcggc tcgaacgga 
      attgtgcgg tatcggctc aaagctgat ccagcagga 
      cactggaaa agcttcaat ttgcaaccg gtctcagga 
      atactagaa ctgcggcta cctttagac cgaaaaaaa 
      cagaaagtg tacgatatt agaatcgaa gcttacgac 
      ttgggagtg ccgtctctc agctctgat ctggatctg 
      acagtttac gtaagcaac atcaacgac taccaaccc 
      cagttcatg ggtgatgaa ttcacagtc aatttcact 
      gaaaacaaa ccatccgaa gtggaaaga caaaaactt 
      ccccaaacc gtcgatcga gatgagctc gaattcgac 
      gggccgttc gcaccaatt tgttatttt atagtgagt 
      aggccttta gatcgagaa gaaaaagac acacattta 
      ttgctcgtc aaagcaaca gaagattgc acaaaaccg 
      ccacgcaac gaaagcttc ttcgattcc tccgatgat 
      acgcaactc aaagtcata gtaaaagtg ttggatgtc 
      aatgataat gccccacag ttcatccac agggttttc 
      accggtgga gtcagtacg gcaacgagc tttggaaca 
      acctttatg gaagtcaag gcggaagat gccgacttg 
      gacgacaac gcccgaatg tcctatttc ctgatcgga 
      aaagtgcaa atgaccctg actgaaggt ttagaagat 
      ttgaaaaga gaccctttt ttggtggat cgggacaca 
      ggagctgtc caattgaat ttcgatcct caggaagga 
      atgaagggt tatttcgac ttcatggtc ctagtgaac 
      gacactgga ggcctccag gacatggct cgggttttc 
      atttatttg cttagggcc gaccaaaga gtccgattt 
      gtcttaaga cagcaacct cccgaattg cgaaataaa 
      attgaggcg tttcgagaa attttgggt aacgtgacc 
      ggagctatt gtgaacgtt gacgaattt cgagttcac 
      gcaaatcat gatggtagc gttgacaaa acccgaacc 
      gatctgtac ctacacttg gttgataga cgcgataat 
      tccataatc gaagttgac gaagtcttg cgcttagtt 
      gaccaaaat accgaaaaa ctcgatgaa ttatttaaa 
      gagtttaac gtgttggac acccaaccg ggaggaccg 
      cttgccttg aaagcggcg aaatcagcc aatacgacg 
      ttctggtta actgctgca actttattc ctactgctc 
      ctcctcttg ttgtgccta gctctttgc ataaaccaa 
      cgacaaacc taccaccgg aaattaaaa gcggccaca 
      gcaaccgca tatgtaact acagattcc gacatagac 
      ggtcggggt ttaagcgct ttgagtgga agagtacca 
      aacacaaat aagcacagc atggaggga agcaacccc 
      atttggatg caagcttat gagaacgaa tggtacaaa 
      aatgcggac gaatttagt caaaattcg gaacacgat 
      tctcttgat gaaaacgtg gtgagctgc ggggatctc 
      ccacagtgc aacaatttg cagcatacc aatggtcac 
      attcgtgcc caaaatgtg taccaaacg ttaccacca 
      gcattgcca agaagaaaa ctcgaaaca acggaacta 
      taatcattt gtaaataaa acaaataat aaatgacat 
      cattttcta ttaaatgac actattgtt tgagtttcg 
      tatttttgg cctgtggca ctttacaac gatttagta 
      aaccaagtg tatttgcgg ttaaaaaat agcaatcta 
      acacattat aagtgtgtt attttacaa ctacataca 
      taatcataa aactgtgta aatactgtg taacataat 
      ttcaagaat aaaacgttt acatggat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY