Status
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Gene information
General information
TC031000
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001123,TC001124,TC001122
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11317883..11345587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031000
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03142

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031000 RA
      MFDIVFVFF LFLVGVTCL VYYLPGRRP EPEDRPLQC 
      YVTVTSADV QGMSRSRIK EARLHGGPS LGQLLTARP 
      SGLTEPECW AILCQAVQA LQDLFLSDG ASGDSCIPL 
      VTPSTLILS SRGRVKLCQ SSTVFGCGP PAHLAGYLA 
      PEYRPNKQC SDTETEKMW IFALGETLK RVTLTSHSA 
      TLRLSNELY QVLVDMTKV HVAARASLM YLLEVISDY 
      CKRRQQNRP FSHIVMDLH QEAMAAVEL ALDTPWGPP 
      AMPELRPRT RNRSESNAD GIGTIPRRW ISLTRPQDP 
      VPKLTKEAS TSMEDLSFS IPISNAPGS LVRPKSMCV 
      PDMRYFNND LPKDFQFCA SATNRLSDL AAGSGPKLS 
      RAERLDGGS SGRRPRMRR NPVQRAASR LYRASVDVD 
      SARDCVGPE FVVRASLPS KQITMPDFK GQKKHVTVI 
      MLNGQKVDV LCNPNTTTA GQLFEAIIQ KEHIEENFM 
      LGLSALIAG DFVFLPSDT RICKAVQAG NAQNATLTL 
      FMRVRFFLP SLRGIRGSQ ARHLLYLQL RRSILEHQL 
      PCSFSQLIE LDGLALQAE FGNYGEREH GARDYFLLE 
      HYVPETMSC AIDDQYGLR QELIRSHIS KRGLSAEKA 
      EQDFIILAQ SLPHYGGHF YTATWMLKD NNHKDVWLY 
      ISAQGINLY ERDRSTSNC GPQLYEMFE WRSIQTLCY 
      SKQYLCILP HSKILHSSK LKKYKLKMD HKKSYFTFR 
      LASLHHQFF LRLRTEYTS LQTLSQQFG IPLKDMKNE 
      TNSLCKLEA LTNPNYMAL DQSTVNAET EFKIEFRAS 
      SKCSSNKAS SENKCRRAK SVSDDINED YQNKENEHP 
      SDRNSSGLV LDSCYLSGL GNINPDKRR GVKMGTRMF 
      SNIGPRVSR SMEAMNAPS HEYLEEMSL QSVSLHCSS 
      TSISRSPTA ECLPTNEAY IIDSSVKSC ANPFLPDFQ 
      ESLSETLLN KLNNISFEE ERILSTVSV ERDCKGSLG 
      LQITEGSDG NVYIQSVIL GGPAHLSGN ILSGDQVVA 
      VDGQSLLGM KYKNALNLL TNTGRKVEF VLSRIAPSR 
      QSTLRIGDA KVNLSNISE SHLKSLRLE NTMNKLKRL 
      SYPNNNRVL VGSPVEKHV TESCLDISN FHKYGSSNT 
      STEVPYHKH IRYEIEPET ENVMARKNL PQSQSNTPV 
      TSLNKNISK SCNHIYDEA DRAVVVDVI PKNGANSRS 
      LDRKLGKSC AQEDNKCKP TIPPIALPR SLGLSRKWR 
      GPVRYPVTP VKKTIGGDL DNHTNYLSN SDDDQVFI
    CDS sequence
    • >TC031000 RA
      atgttcgac atcgtgttc gtgttcttc ttgttcctg 
      gtgggggtc acctgtctc gtgtactac ctgccgggg 
      aggcgaccc gaacctgaa gaccgacct ctgcagtgc 
      tacgttacc gtcacgtcg gcggacgtc caaggcatg 
      tccaggtcc cgcatcaaa gaagctcgt cttcatggg 
      ggtccaagc ttgggccaa ctcttgacg gcacgtccc 
      tcgggcctt acggagccc gagtgttgg gctatcttg 
      tgtcaagcc gttcaagca ctgcaggat ttattctta 
      tctgatggt gcctcaggt gattcatgc attcctttg 
      gtaacaccc tctaccctc atactgtca tccagagga 
      agggtgaaa ctatgccag agcagcaca gtgtttgga 
      tgtggtccc ccagcacac ctggcaggt tatttagcg 
      cctgaatac aggcctaac aaacagtgc tctgacacg 
      gagacggaa aagatgtgg atattcgcc ctgggggaa 
      actctgaaa agggttact ttaacgagc cattctgcc 
      actttacga ttgagtaat gaactgtat caagtgctg 
      gttgacatg accaaagtg cacgtggct gccagggcg 
      tctctcatg tatctatta gaggttatt tctgactac 
      tgtaaacgc cgccaacag aacaggcct ttttcccac 
      atcgtgatg gatttgcat caggaagca atggcagcc 
      gtagagtta gccctcgac actccttgg ggaccccca 
      gctatgcca gaattgcgt cccagaacc agaaacaga 
      agtgagtcg aacgcggac ggcatcggc accatcccc 
      cggcgctgg atcagcctg acgcgcccc caggacccc 
      gtccccaag ctgacaaaa gaagccagc accagcatg 
      gaggacctg tctttttcc atcccaatt tcgaacgcc 
      cccggctcg ctcgtgcgc cccaaatcg atgtgtgtg 
      cctgacatg cgatacttt aacaatgat ctacctaag 
      gattttcaa ttttgcgcg agtgcgacg aacagattg 
      agtgatttg gccgccggt tcgggcccc aaactgagt 
      cgcgccgag cgtctggac ggcggcagc agcggcaga 
      aggcccaga atgaggcgc aatccggtg cagcgggcg 
      gcctccaga ctctaccgg gccagtgtg gacgttgac 
      agcgccagg gactgcgta gggccggag ttcgttgta 
      agggcgtcg ctgcccagt aagcagatt accatgccc 
      gatttcaaa ggacaaaag aaacatgtg acggttatc 
      atgttgaat gggcagaaa gttgacgtt ctctgcaat 
      ccgaatacc accactgcc ggacaactg ttcgaggca 
      ataatccag aaagaacac atcgaagag aactttatg 
      ctgggcctt tcggcactg atagccggc gattttgtt 
      tttctaccc tcagacaca cgaatttgc aaggcggtg 
      caagctggc aatgcgcaa aatgcgact ctgacttta 
      ttcatgcga gttcgcttc tttctgcca agtcttcga 
      gggattaga ggatcacaa gcccgtcat ttgctgtat 
      ttgcaattg agacggtcg attttagaa caccaattg 
      ccatgttct ttctctcag ctcatcgaa ctggacgga 
      cttgccttg caggcggag ttcgggaat tatggcgaa 
      agggagcac ggggctcgg gattacttc ctgttggaa 
      cattacgtt cctgagacc atgtcgtgc gcgattgat 
      gatcagtat ggcttgcga caagaattg atcaggtct 
      catatatct aaacgggga ttaagtgcg gaaaaggct 
      gagcaggac tttattatc cttgcccaa agtcttccc 
      cattacggt ggacatttt tacacagca acttggatg 
      ttaaaagac aataatcat aaagacgtt tggctgtat 
      atcagcgcc caagggata aatctatac gaacgtgac 
      agatcaaca agcaactgt ggaccacaa ttatacgaa 
      atgttcgaa tggcgaagc attcaaact ttgtgctac 
      agcaaacaa tacttatgc atccttccg cattcgaaa 
      attctacac tcgtcgaaa ttaaagaaa tataagctg 
      aaaatggac cataaaaag agttatttc actttccgt 
      ctcgcttca ttacaccac caatttttc ctgcgtctc 
      cgcactgaa tatacgtcg ctccagacg ctctcgcaa 
      caattcggc atcccgctc aaagacatg aaaaatgaa 
      acaaactct ttgtgcaaa ttagaagca ctaacaaac 
      ccaaattac atggctttg gaccagtcg acagtgaac 
      gccgagacg gagttcaaa atcgaattc cgagcctct 
      tcgaaatgt agcagtaat aaagcctca agtgagaat 
      aaatgccgg agagcgaaa agtgtgagc gacgatatc 
      aatgaagac tatcaaaat aaggaaaac gagcatcct 
      tccgataga aacagctcg ggattagtc cttgatagt 
      tgttatcta tcagggctg ggaaatata aatcccgat 
      aagaggcga ggagttaaa atggggact aggatgttc 
      agcaacatt ggacctcga gtgtcgaga tctatggag 
      gcaatgaat gcgccaagt cacgagtat ttggaagag 
      atgtcgttg caatcagtc tctctacat tgcagttcg 
      acgtcgata tcacggtcc ccaaccgcg gagtgcctc 
      ccaacgaac gaggcctat attatagat tcctcggtg 
      aagtcgtgc gccaacccg tttctgccc gacttccaa 
      gagagttta agtgagact ttactcaac aaactgaat 
      aacatttcg ttcgaagag gagcgcatt ttatcgacc 
      gtttcagtg gagcgggac tgtaagggt agtttgggg 
      ctgcagata acagagggc tccgacggc aacgtctac 
      atccagtcg gtgatctta ggcggccct gcccaccta 
      agtgggaat atattgagc ggtgaccaa gtggttgct 
      gtagacggc caaagtctc ttagggatg aaatacaag 
      aatgcctta aacttgcta acaaataca gggcgaaaa 
      gtggaattt gtgctatcg cgaattgcg ccctccagg 
      cagtcgact ttgcgtatt ggcgacgct aaagtgaac 
      ttatccaac atcagcgaa tcgcacttg aagtcgctc 
      cgcctggag aacaccatg aacaaactc aagcgccta 
      tcgtacccc aataacaac agggttcta gttggtagt 
      ccggtcgag aaacacgtg acggagagt tgcctcgat 
      atttccaat tttcacaaa tatgggtct agtaatact 
      tcaacggaa gtgccatat cataaacat atccgctat 
      gaaatcgag cccgagacc gagaatgtg atggcgagg 
      aagaatctg ccgcagagc caaagcaat actccagtg 
      acgagtctg aataaaaat atttccaag tcgtgcaat 
      cacatctat gatgaggcg gacagagcg gttgtggtg 
      gatgtgata cccaagaac ggggccaac tcgaggtca 
      ctcgatcgc aaacttggc aaaagttgc gcccaggaa 
      gataataaa tgcaaacct actataccg cctatagcg 
      ctacctcga agtctagga ctcagcagg aagtggagg 
      gggcccgtc aggtacccc gtaacacca gtgaaaaaa 
      acaatcggg ggtgacctg gacaatcac accaattac 
      ttgtccaac tcagacgac gaccaagtt tttatttag
    mRNA sequence
    • >TC031000 RA
      ctcacgtgg aactccctc atcatgttc gacatcgtg 
      ttcgtgttc ttcttgttc ctggtgggg gtcacctgt 
      ctcgtgtac tacctgccg gggaggcga cccgaacct 
      gaagaccga cctctgcag tgctacgtt accgtcacg 
      tcggcggac gtccaaggc atgtccagg tcccgcatc 
      aaagaagct cgtcttcat gggggtcca agcttgggc 
      caactcttg acggcacgt ccctcgggc cttacggag 
      cccgagtgt tgggctatc ttgtgtcaa gccgttcaa 
      gcactgcag gatttattc ttatctgat ggtgcctca 
      ggtgattca tgcattcct ttggtaaca ccctctacc 
      ctcatactg tcatccaga ggaagggtg aaactatgc 
      cagagcagc acagtgttt ggatgtggt cccccagca 
      cacctggca ggttattta gcgcctgaa tacaggcct 
      aacaaacag tgctctgac acggagacg gaaaagatg 
      tggatattc gccctgggg gaaactctg aaaagggtt 
      actttaacg agccattct gccacttta cgattgagt 
      aatgaactg tatcaagtg ctggttgac atgaccaaa 
      gtgcacgtg gctgccagg gcgtctctc atgtatcta 
      ttagaggtt atttctgac tactgtaaa cgccgccaa 
      cagaacagg cctttttcc cacatcgtg atggatttg 
      catcaggaa gcaatggca gccgtagag ttagccctc 
      gacactcct tggggaccc ccagctatg ccagaattg 
      cgtcccaga accagaaac agaagtgag tcgaacgcg 
      gacggcatc ggcaccatc ccccggcgc tggatcagc 
      ctgacgcgc ccccaggac cccgtcccc aagctgaca 
      aaagaagcc agcaccagc atggaggac ctgtctttt 
      tccatccca atttcgaac gcccccggc tcgctcgtg 
      cgccccaaa tcgatgtgt gtgcctgac atgcgatac 
      tttaacaat gatctacct aaggatttt caattttgc 
      gcgagtgcg acgaacaga ttgagtgat ttggccgcc 
      ggttcgggc cccaaactg agtcgcgcc gagcgtctg 
      gacggcggc agcagcggc agaaggccc agaatgagg 
      cgcaatccg gtgcagcgg gcggcctcc agactctac 
      cgggccagt gtggacgtt gacagcgcc agggactgc 
      gtagggccg gagttcgtt gtaagggcg tcgctgccc 
      agtaagcag attaccatg cccgatttc aaaggacaa 
      aagaaacat gtgacggtt atcatgttg aatgggcag 
      aaagttgac gttctctgc aatccgaat accaccact 
      gccggacaa ctgttcgag gcaataatc cagaaagaa 
      cacatcgaa gagaacttt atgctgggc ctttcggca 
      ctgatagcc ggcgatttt gtttttcta ccctcagac 
      acacgaatt tgcaaggcg gtgcaagct ggcaatgcg 
      caaaatgcg actctgact ttattcatg cgagttcgc 
      ttctttctg ccaagtctt cgagggatt agaggatca 
      caagcccgt catttgctg tatttgcaa ttgagacgg 
      tcgatttta gaacaccaa ttgccatgt tctttctct 
      cagctcatc gaactggac ggacttgcc ttgcaggcg 
      gagttcggg aattatggc gaaagggag cacggggct 
      cgggattac ttcctgttg gaacattac gttcctgag 
      accatgtcg tgcgcgatt gatgatcag tatggcttg 
      cgacaagaa ttgatcagg tctcatata tctaaacgg 
      ggattaagt gcggaaaag gctgagcag gactttatt 
      atccttgcc caaagtctt ccccattac ggtggacat 
      ttttacaca gcaacttgg atgttaaaa gacaataat 
      cataaagac gtttggctg tatatcagc gcccaaggg 
      ataaatcta tacgaacgt gacagatca acaagcaac 
      tgtggacca caattatac gaaatgttc gaatggcga 
      agcattcaa actttgtgc tacagcaaa caatactta 
      tgcatcctt ccgcattcg aaaattcta cactcgtcg 
      aaattaaag aaatataag ctgaaaatg gaccataaa 
      aagagttat ttcactttc cgtctcgct tcattacac 
      caccaattt ttcctgcgt ctccgcact gaatatacg 
      tcgctccag acgctctcg caacaattc ggcatcccg 
      ctcaaagac atgaaaaat gaaacaaac tctttgtgc 
      aaattagaa gcactaaca aacccaaat tacatggct 
      ttggaccag tcgacagtg aacgccgag acggagttc 
      aaaatcgaa ttccgagcc tcttcgaaa tgtagcagt 
      aataaagcc tcaagtgag aataaatgc cggagagcg 
      aaaagtgtg agcgacgat atcaatgaa gactatcaa 
      aataaggaa aacgagcat ccttccgat agaaacagc 
      tcgggatta gtccttgat agttgttat ctatcaggg 
      ctgggaaat ataaatccc gataagagg cgaggagtt 
      aaaatgggg actaggatg ttcagcaac attggacct 
      cgagtgtcg agatctatg gaggcaatg aatgcgcca 
      agtcacgag tatttggaa gagatgtcg ttgcaatca 
      gtctctcta cattgcagt tcgacgtcg atatcacgg 
      tccccaacc gcggagtgc ctcccaacg aacgaggcc 
      tatattata gattcctcg gtgaagtcg tgcgccaac 
      ccgtttctg cccgacttc caagagagt ttaagtgag 
      actttactc aacaaactg aataacatt tcgttcgaa 
      gaggagcgc attttatcg accgtttca gtggagcgg 
      gactgtaag ggtagtttg gggctgcag ataacagag 
      ggctccgac ggcaacgtc tacatccag tcggtgatc 
      ttaggcggc cctgcccac ctaagtggg aatatattg 
      agcggtgac caagtggtt gctgtagac ggccaaagt 
      ctcttaggg atgaaatac aagaatgcc ttaaacttg 
      ctaacaaat acagggcga aaagtggaa tttgtgcta 
      tcgcgaatt gcgccctcc aggcagtcg actttgcgt 
      attggcgac gctaaagtg aacttatcc aacatcagc 
      gaatcgcac ttgaagtcg ctccgcctg gagaacacc 
      atgaacaaa ctcaagcgc ctatcgtac cccaataac 
      aacagggtt ctagttggt agtccggtc gagaaacac 
      gtgacggag agttgcctc gatatttcc aattttcac 
      aaatatggg tctagtaat acttcaacg gaagtgcca 
      tatcataaa catatccgc tatgaaatc gagcccgag 
      accgagaat gtgatggcg aggaagaat ctgccgcag 
      agccaaagc aatactcca gtgacgagt ctgaataaa 
      aatatttcc aagtcgtgc aatcacatc tatgatgag 
      gcggacaga gcggttgtg gtggatgtg atacccaag 
      aacggggcc aactcgagg tcactcgat cgcaaactt 
      ggcaaaagt tgcgcccag gaagataat aaatgcaaa 
      cctactata ccgcctata gcgctacct cgaagtcta 
      ggactcagc aggaagtgg agggggccc gtcaggtac 
      cccgtaaca ccagtgaaa aaaacaatc gggggtgac 
      ctggacaat cacaccaat tacttgtcc aactcagac 
      gacgaccaa gtttttatt tagttttac gtcaaactg 
      cggcagatt aattttttt ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY