Status
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Gene information
General information
TC001121 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11290812..11294503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001121
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001121 RA
      MADLTKITP LFLLLLLCK KSLCAESES KEVLVESES 
      KDGKLEIIK QIKKVNDDG SYTIGYEAD DGSFKIESR 
      DVLGNIKGT YGYIDDAGQ IKRVSYSTS NSSEILPKP 
      DQPSVVQRI PKTNRTQIY TSSTKRPFY STTQSTPTS 
      TTALQRLAK LRSQSTTTT EKPNYSEVV NMESEIKTT 
      SKPHIVYPS STPRILLQG RSTVRSTAA THTQTQKSE 
      GQLIRPEIV TARPTELPL FRRLALKHP LLGDDKHVA 
      DGSEVRSNI LRRQLSQEK SDFDPQQHV FTLQQALGS 
      DATDVYSGS VTTGTPRPL FTTTSRPRA LLSTTTTPT 
      VVSRPTIRY PVNYQRTVL QDLESNTQY AQETTTTEA 
      TVNDATPTP VPVVQIPAN RANQEPLVA IRHPFQRGT 
      ILVPLSQLQ GRVVPVDIN DNLSQYGRQ NEQNTQKPD 
      VEPQPIYVR RLPPPQFRP IPVQVDENG YIRDLQRQP 
      NGQPIPYPV PVPPQKYND VDNDIDEIS PPVSTRDFQ 
      KLLHQLILR QTRLERISA LTDPRRHPE LYQPRYRPV 
      YRPTPVPYY QQNGPVQFL SDDNVQQRQ RQYVHISPE 
      QQVAIRQQQ RQDQEVYQN GQSYIPMRR VARLLQPGE 
      DQRLENKDE YLPPDVREM LLLRMLQLA INPALPLSE 
      DEMVQGTTP VPQYKKAPV RNVEILGEE PEEKRPVRS 
      KRYREPHMD YYEQ
    CDS sequence
    • >TC001121 RA
      atggcagat ttaacaaag attactccg ttgtttttg 
      ttgctcctt ctttgcaaa aagtcactc tgtgcagag 
      agtgaatcg aaggaagta ctagtcgag tcagagtca 
      aaggacggc aagcttgaa ataatcaaa caaattaag 
      aaagtcaat gacgatggc tcctacact ataggttac 
      gaagccgat gatgggtcg ttcaaaatc gaaagtcgg 
      gacgtttta gggaatatt aagggaact tacgggtat 
      atagacgac gctggacaa attaaaaga gtgtcttac 
      agcacgagc aattctagt gagatttta ccaaaaccg 
      gatcaacca tccgtggtt caaagaatc cccaagaca 
      aatcgcacc caaatttac acttcgtca accaaacgc 
      ccgttttat tccacaact cagtcgacc ccaaccagc 
      acaacggcc ctccaaaga ctcgccaaa ctgcgcagt 
      cagtcaaca acaaccacc gaaaaacca aactacagc 
      gaagtggtt aacatggaa agcgaaatc aaaaccaca 
      agcaaaccg catatagtg tacccaagc tcgacaccc 
      agaatcttg ctacaaggt agatcaaca gttagatca 
      accgcagca acacacacc caaacccaa aaatccgaa 
      ggtcagcta attagacca gaaattgta acagctagg 
      ccaacagaa ctgccattg ttcagacga cttgctttg 
      aaacaccca cttttgggt gacgacaaa cacgtggcg 
      gatggatcc gaagtcagg agtaacatc ttgcggaga 
      caattgtcc caagagaaa tctgacttt gatccccaa 
      cagcacgtt ttcacgcta cagcaagct ttaggtagc 
      gacgcgact gacgtttac agtggttcc gtaaccact 
      ggaacccca cgacctttg ttcactact acgtcccga 
      cctagggct cttttaagt acgaccaca acaccaaca 
      gtggtctcg agaccaact atacggtat ccagtaaac 
      taccaaagg actgtatta caagacttg gaaagcaac 
      acacagtat gcgcaagaa acaacaacc acggaagca 
      acggttaat gacgcgacc ccaaccccg gtcccagtg 
      gtccaaatc ccggcgaac agagccaac caagagccc 
      cttgttgca ataagacac ccgttccaa aggggtact 
      attttggta ccgttgagc caattgcaa ggacgagtg 
      gttccagtt gatatcaat gacaatctc agtcaatac 
      ggaagacaa aatgaacaa aacactcaa aaaccggat 
      gttgagccg cagcctatt tacgtgcgg aggttgcca 
      ccgccccag tttagacca attccggtc caagtcgac 
      gaaaatgga tatatccga gacttgcaa aggcaacca 
      aacggccaa cccattccg taccctgtc ccagtcccg 
      ccccaaaaa tacaatgat gtggacaac gatattgac 
      gaaatcagc cctcctgtc agtacccga gacttccaa 
      aagctcctg caccaatta attttgcgc caaacccgc 
      ttggaacgc atcagtgct ttgactgac ccgagacgc 
      cacccagaa ttataccaa ccgaggtat aggccagtc 
      tatcgccca acacctgtc ccatattac caacaaaac 
      ggcccagta caattttta tccgacgat aatgtgcaa 
      caaaggcaa agacaatac gtccacatt tcgccagaa 
      cagcaagtt gcaattcgt cagcaacaa cgccaagac 
      caagaagtg tatcagaac gggcagtct tacattccc 
      atgagacga gtggcgagg cttctgcaa ccaggggaa 
      gaccaacgt cttgaaaat aaagacgaa tatcttccc 
      ccagatgtt agggaaatg ctcttgttg cggatgctc 
      caacttgcc ataaaccca gcattacca ctgagcgag 
      gatgagatg gtgcaagga acaaccccc gtgccacaa 
      tacaagaag gctccagtt cggaatgtg gaaattttg 
      ggggaagaa cccgaagag aaacggccc gtgagaagt 
      aaaaggtac agagagcca catatggat tattatgaa 
      caatag
    mRNA sequence
    • >TC001121 RA
      tgtcttaag ttatatagg gaaccggcg gtttaaatg 
      aagcagtcg gtgtaaaca cgtggtgta agcaactgc 
      cgcaatggc agatttaac aaagattac tccgttgtt 
      tttgttgct ccttctttg caaaaagtc actctgtgc 
      agagagtga atcgaagga agtactagt cgagtcaga 
      gtcaaagga cggcaagct tgaaataat caaacaaat 
      taagaaagt caatgacga tggctccta cactatagg 
      ttacgaagc cgatgatgg gtcgttcaa aatcgaaag 
      tcgggacgt tttagggaa tattaaggg aacttacgg 
      gtatataga cgacgctgg acaaattaa aagagtgtc 
      ttacagcac gagcaattc tagtgagat tttaccaaa 
      accggatca accatccgt ggttcaaag aatccccaa 
      gacaaatcg cacccaaat ttacacttc gtcaaccaa 
      acgcccgtt ttattccac aactcagtc gaccccaac 
      cagcacaac ggccctcca aagactcgc caaactgcg 
      cagtcagtc aacaacaac caccgaaaa accaaacta 
      cagcgaagt ggttaacat ggaaagcga aatcaaaac 
      cacaagcaa accgcatat agtgtaccc aagctcgac 
      acccagaat cttgctaca aggtagatc aacagttag 
      atcaaccgc agcaacaca cacccaaac ccaaaaatc 
      cgaaggtca gctaattag accagaaat tgtaacagc 
      taggccaac agaactgcc attgttcag acgacttgc 
      tttgaaaca cccactttt gggtgacga caaacacgt 
      ggcggatgg atccgaagt caggagtaa catcttgcg 
      gagacaatt gtcccaaga gaaatctga ctttgatcc 
      ccaacagca cgttttcac gctacagca agctttagg 
      tagcgacgc gactgacgt ttacagtgg ttccgtaac 
      cactggaac cccacgacc tttgttcac tactacgtc 
      ccgacctag ggctctttt aagtacgac cacaacacc 
      aacagtggt ctcgagacc aactatacg gtatccagt 
      aaactacca aaggactgt attacaaga cttggaaag 
      caacacaca gtatgcgca agaaacaac aaccacgga 
      agcaacggt taatgacgc gaccccaac cccggtccc 
      agtggtcca aatcccggc gaacagagc caaccaaga 
      gccccttgt tgcaataag acacccgtt ccaaagggg 
      tactatttt ggtaccgtt gagccaatt gcaaggacg 
      agtggttcc agttgatat caatgacaa tctcagtca 
      atacggaag acaaaatga acaaaacac tcaaaaacc 
      ggatgttga gccgcagcc tatttacgt gcggaggtt 
      gccaccgcc ccagtttag accaattcc ggtccaagt 
      cgacgaaaa tggatatat ccgagactt gcaaaggca 
      accaaacgg ccaacccat tccgtaccc tgtcccagt 
      cccgcccca aaaatacaa tgatgtgga caacgatat 
      tgacgaaat cagccctcc tgtcagtac ccgagactt 
      ccaaaagct cctgcacca attaatttt gcgccaaac 
      ccgcttgga acgcatcag tgctttgac tgacccgag 
      acgccaccc agaattata ccaaccgag gtataggcc 
      agtctatcg cccaacacc tgtcccata ttaccaaca 
      aaacggccc agtacaatt tttatccga cgataatgt 
      gcaacaaag gcaaagaca atacgtcca catttcgcc 
      agaacagca agttgcaat tcgtcagca acaacgcca 
      agaccaaga agtgtatca gaacgggca gtcttacat 
      tcccatgag acgagtggc gaggcttct gcaaccagg 
      ggaagacca acgtcttga aaataaaga cgaatatct 
      tcccccaga tgttaggga aatgctctt gttgcggat 
      gctccaact tgccataaa cccagcatt accactgag 
      cgaggatga gatggtgca aggaacaac ccccgtgcc 
      acaatacaa gaaggctcc agttcggaa tgtggaaat 
      tttggggga agaacccga agagaaacg gcccgtgag 
      aagtaaaag gtacagaga gccacatat ggattatta 
      tgaacaata gtagcgctt gttgcgtgt tgtacatac 
      aaacggtat actatctta tcttaatga ttacgggac 
      aatgacagt tttcgttgc aaaaatgca aagtaggta 
      ttaaaatca tcttagttc acagtcatg ttagtacat 
      gaggtttat ttattagca tgctcatta tcattacct 
      acttttcca atttattta tgttgcaaa tttcgcgga 
      cgtcagcgc acctggcgt tgttttggt gatattttt 
      gtagatatt tattgttca ttttttact tgttttgta 
      ccctagtta ggccacgca taattaata gattgcatg 
      cttcaatta tgtaaatat tgcgcaacc attaccaaa 
      gatgtgaat actttacgt gggaactta tctacatgt 
      cgtttcttt ttaaacaac tgttctatt attacaata 
      aaacaatta atgaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY