| General information | |||
| TC001121 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11290812..11294503 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001121 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03141 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001121 RA
MADLTKITP LFLLLLLCK KSLCAESES KEVLVESES KDGKLEIIK QIKKVNDDG SYTIGYEAD DGSFKIESR DVLGNIKGT YGYIDDAGQ IKRVSYSTS NSSEILPKP DQPSVVQRI PKTNRTQIY TSSTKRPFY STTQSTPTS TTALQRLAK LRSQSTTTT EKPNYSEVV NMESEIKTT SKPHIVYPS STPRILLQG RSTVRSTAA THTQTQKSE GQLIRPEIV TARPTELPL FRRLALKHP LLGDDKHVA DGSEVRSNI LRRQLSQEK SDFDPQQHV FTLQQALGS DATDVYSGS VTTGTPRPL FTTTSRPRA LLSTTTTPT VVSRPTIRY PVNYQRTVL QDLESNTQY AQETTTTEA TVNDATPTP VPVVQIPAN RANQEPLVA IRHPFQRGT ILVPLSQLQ GRVVPVDIN DNLSQYGRQ NEQNTQKPD VEPQPIYVR RLPPPQFRP IPVQVDENG YIRDLQRQP NGQPIPYPV PVPPQKYND VDNDIDEIS PPVSTRDFQ KLLHQLILR QTRLERISA LTDPRRHPE LYQPRYRPV YRPTPVPYY QQNGPVQFL SDDNVQQRQ RQYVHISPE QQVAIRQQQ RQDQEVYQN GQSYIPMRR VARLLQPGE DQRLENKDE YLPPDVREM LLLRMLQLA INPALPLSE DEMVQGTTP VPQYKKAPV RNVEILGEE PEEKRPVRS KRYREPHMD YYEQ
- >TC001121 RA
atggcagat ttaacaaag attactccg ttgtttttg ttgctcctt ctttgcaaa aagtcactc tgtgcagag agtgaatcg aaggaagta ctagtcgag tcagagtca aaggacggc aagcttgaa ataatcaaa caaattaag aaagtcaat gacgatggc tcctacact ataggttac gaagccgat gatgggtcg ttcaaaatc gaaagtcgg gacgtttta gggaatatt aagggaact tacgggtat atagacgac gctggacaa attaaaaga gtgtcttac agcacgagc aattctagt gagatttta ccaaaaccg gatcaacca tccgtggtt caaagaatc cccaagaca aatcgcacc caaatttac acttcgtca accaaacgc ccgttttat tccacaact cagtcgacc ccaaccagc acaacggcc ctccaaaga ctcgccaaa ctgcgcagt cagtcaaca acaaccacc gaaaaacca aactacagc gaagtggtt aacatggaa agcgaaatc aaaaccaca agcaaaccg catatagtg tacccaagc tcgacaccc agaatcttg ctacaaggt agatcaaca gttagatca accgcagca acacacacc caaacccaa aaatccgaa ggtcagcta attagacca gaaattgta acagctagg ccaacagaa ctgccattg ttcagacga cttgctttg aaacaccca cttttgggt gacgacaaa cacgtggcg gatggatcc gaagtcagg agtaacatc ttgcggaga caattgtcc caagagaaa tctgacttt gatccccaa cagcacgtt ttcacgcta cagcaagct ttaggtagc gacgcgact gacgtttac agtggttcc gtaaccact ggaacccca cgacctttg ttcactact acgtcccga cctagggct cttttaagt acgaccaca acaccaaca gtggtctcg agaccaact atacggtat ccagtaaac taccaaagg actgtatta caagacttg gaaagcaac acacagtat gcgcaagaa acaacaacc acggaagca acggttaat gacgcgacc ccaaccccg gtcccagtg gtccaaatc ccggcgaac agagccaac caagagccc cttgttgca ataagacac ccgttccaa aggggtact attttggta ccgttgagc caattgcaa ggacgagtg gttccagtt gatatcaat gacaatctc agtcaatac ggaagacaa aatgaacaa aacactcaa aaaccggat gttgagccg cagcctatt tacgtgcgg aggttgcca ccgccccag tttagacca attccggtc caagtcgac gaaaatgga tatatccga gacttgcaa aggcaacca aacggccaa cccattccg taccctgtc ccagtcccg ccccaaaaa tacaatgat gtggacaac gatattgac gaaatcagc cctcctgtc agtacccga gacttccaa aagctcctg caccaatta attttgcgc caaacccgc ttggaacgc atcagtgct ttgactgac ccgagacgc cacccagaa ttataccaa ccgaggtat aggccagtc tatcgccca acacctgtc ccatattac caacaaaac ggcccagta caattttta tccgacgat aatgtgcaa caaaggcaa agacaatac gtccacatt tcgccagaa cagcaagtt gcaattcgt cagcaacaa cgccaagac caagaagtg tatcagaac gggcagtct tacattccc atgagacga gtggcgagg cttctgcaa ccaggggaa gaccaacgt cttgaaaat aaagacgaa tatcttccc ccagatgtt agggaaatg ctcttgttg cggatgctc caacttgcc ataaaccca gcattacca ctgagcgag gatgagatg gtgcaagga acaaccccc gtgccacaa tacaagaag gctccagtt cggaatgtg gaaattttg ggggaagaa cccgaagag aaacggccc gtgagaagt aaaaggtac agagagcca catatggat tattatgaa caatag
- >TC001121 RA
tgtcttaag ttatatagg gaaccggcg gtttaaatg aagcagtcg gtgtaaaca cgtggtgta agcaactgc cgcaatggc agatttaac aaagattac tccgttgtt tttgttgct ccttctttg caaaaagtc actctgtgc agagagtga atcgaagga agtactagt cgagtcaga gtcaaagga cggcaagct tgaaataat caaacaaat taagaaagt caatgacga tggctccta cactatagg ttacgaagc cgatgatgg gtcgttcaa aatcgaaag tcgggacgt tttagggaa tattaaggg aacttacgg gtatataga cgacgctgg acaaattaa aagagtgtc ttacagcac gagcaattc tagtgagat tttaccaaa accggatca accatccgt ggttcaaag aatccccaa gacaaatcg cacccaaat ttacacttc gtcaaccaa acgcccgtt ttattccac aactcagtc gaccccaac cagcacaac ggccctcca aagactcgc caaactgcg cagtcagtc aacaacaac caccgaaaa accaaacta cagcgaagt ggttaacat ggaaagcga aatcaaaac cacaagcaa accgcatat agtgtaccc aagctcgac acccagaat cttgctaca aggtagatc aacagttag atcaaccgc agcaacaca cacccaaac ccaaaaatc cgaaggtca gctaattag accagaaat tgtaacagc taggccaac agaactgcc attgttcag acgacttgc tttgaaaca cccactttt gggtgacga caaacacgt ggcggatgg atccgaagt caggagtaa catcttgcg gagacaatt gtcccaaga gaaatctga ctttgatcc ccaacagca cgttttcac gctacagca agctttagg tagcgacgc gactgacgt ttacagtgg ttccgtaac cactggaac cccacgacc tttgttcac tactacgtc ccgacctag ggctctttt aagtacgac cacaacacc aacagtggt ctcgagacc aactatacg gtatccagt aaactacca aaggactgt attacaaga cttggaaag caacacaca gtatgcgca agaaacaac aaccacgga agcaacggt taatgacgc gaccccaac cccggtccc agtggtcca aatcccggc gaacagagc caaccaaga gccccttgt tgcaataag acacccgtt ccaaagggg tactatttt ggtaccgtt gagccaatt gcaaggacg agtggttcc agttgatat caatgacaa tctcagtca atacggaag acaaaatga acaaaacac tcaaaaacc ggatgttga gccgcagcc tatttacgt gcggaggtt gccaccgcc ccagtttag accaattcc ggtccaagt cgacgaaaa tggatatat ccgagactt gcaaaggca accaaacgg ccaacccat tccgtaccc tgtcccagt cccgcccca aaaatacaa tgatgtgga caacgatat tgacgaaat cagccctcc tgtcagtac ccgagactt ccaaaagct cctgcacca attaatttt gcgccaaac ccgcttgga acgcatcag tgctttgac tgacccgag acgccaccc agaattata ccaaccgag gtataggcc agtctatcg cccaacacc tgtcccata ttaccaaca aaacggccc agtacaatt tttatccga cgataatgt gcaacaaag gcaaagaca atacgtcca catttcgcc agaacagca agttgcaat tcgtcagca acaacgcca agaccaaga agtgtatca gaacgggca gtcttacat tcccatgag acgagtggc gaggcttct gcaaccagg ggaagacca acgtcttga aaataaaga cgaatatct tcccccaga tgttaggga aatgctctt gttgcggat gctccaact tgccataaa cccagcatt accactgag cgaggatga gatggtgca aggaacaac ccccgtgcc acaatacaa gaaggctcc agttcggaa tgtggaaat tttggggga agaacccga agagaaacg gcccgtgag aagtaaaag gtacagaga gccacatat ggattatta tgaacaata gtagcgctt gttgcgtgt tgtacatac aaacggtat actatctta tcttaatga ttacgggac aatgacagt tttcgttgc aaaaatgca aagtaggta ttaaaatca tcttagttc acagtcatg ttagtacat gaggtttat ttattagca tgctcatta tcattacct acttttcca atttattta tgttgcaaa tttcgcgga cgtcagcgc acctggcgt tgttttggt gatattttt gtagatatt tattgttca ttttttact tgttttgta ccctagtta ggccacgca taattaata gattgcatg cttcaatta tgtaaatat tgcgcaacc attaccaaa gatgtgaat actttacgt gggaactta tctacatgt cgtttcttt ttaaacaac tgttctatt attacaata aaacaatta atgaata
