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Gene information
General information
TC001121 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11290812..11294503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001121
    iBeetle screen iB_03141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001121 RA
      MADLTKITP LFLLLLLCK KSLCAESES KEVLVESES 
KDGKLEIIK QIKKVNDDG SYTIGYEAD DGSFKIESR 
DVLGNIKGT YGYIDDAGQ IKRVSYSTS NSSEILPKP 
DQPSVVQRI PKTNRTQIY TSSTKRPFY STTQSTPTS 
TTALQRLAK LRSQSTTTT EKPNYSEVV NMESEIKTT 
SKPHIVYPS STPRILLQG RSTVRSTAA THTQTQKSE 
GQLIRPEIV TARPTELPL FRRLALKHP LLGDDKHVA 
DGSEVRSNI LRRQLSQEK SDFDPQQHV FTLQQALGS 
DATDVYSGS VTTGTPRPL FTTTSRPRA LLSTTTTPT 
VVSRPTIRY PVNYQRTVL QDLESNTQY AQETTTTEA 
TVNDATPTP VPVVQIPAN RANQEPLVA IRHPFQRGT 
ILVPLSQLQ GRVVPVDIN DNLSQYGRQ NEQNTQKPD 
VEPQPIYVR RLPPPQFRP IPVQVDENG YIRDLQRQP 
NGQPIPYPV PVPPQKYND VDNDIDEIS PPVSTRDFQ 
KLLHQLILR QTRLERISA LTDPRRHPE LYQPRYRPV 
YRPTPVPYY QQNGPVQFL SDDNVQQRQ RQYVHISPE 
QQVAIRQQQ RQDQEVYQN GQSYIPMRR VARLLQPGE 
DQRLENKDE YLPPDVREM LLLRMLQLA INPALPLSE 
DEMVQGTTP VPQYKKAPV RNVEILGEE PEEKRPVRS 
KRYREPHMD YYEQ
    CDS sequence
    • >TC001121 RA
      atggcagat ttaacaaag attactccg ttgtttttg 
ttgctcctt ctttgcaaa aagtcactc tgtgcagag 
agtgaatcg aaggaagta ctagtcgag tcagagtca 
aaggacggc aagcttgaa ataatcaaa caaattaag 
aaagtcaat gacgatggc tcctacact ataggttac 
gaagccgat gatgggtcg ttcaaaatc gaaagtcgg 
gacgtttta gggaatatt aagggaact tacgggtat 
atagacgac gctggacaa attaaaaga gtgtcttac 
agcacgagc aattctagt gagatttta ccaaaaccg 
gatcaacca tccgtggtt caaagaatc cccaagaca 
aatcgcacc caaatttac acttcgtca accaaacgc 
ccgttttat tccacaact cagtcgacc ccaaccagc 
acaacggcc ctccaaaga ctcgccaaa ctgcgcagt 
cagtcaaca acaaccacc gaaaaacca aactacagc 
gaagtggtt aacatggaa agcgaaatc aaaaccaca 
agcaaaccg catatagtg tacccaagc tcgacaccc 
agaatcttg ctacaaggt agatcaaca gttagatca 
accgcagca acacacacc caaacccaa aaatccgaa 
ggtcagcta attagacca gaaattgta acagctagg 
ccaacagaa ctgccattg ttcagacga cttgctttg 
aaacaccca cttttgggt gacgacaaa cacgtggcg 
gatggatcc gaagtcagg agtaacatc ttgcggaga 
caattgtcc caagagaaa tctgacttt gatccccaa 
cagcacgtt ttcacgcta cagcaagct ttaggtagc 
gacgcgact gacgtttac agtggttcc gtaaccact 
ggaacccca cgacctttg ttcactact acgtcccga 
cctagggct cttttaagt acgaccaca acaccaaca 
gtggtctcg agaccaact atacggtat ccagtaaac 
taccaaagg actgtatta caagacttg gaaagcaac 
acacagtat gcgcaagaa acaacaacc acggaagca 
acggttaat gacgcgacc ccaaccccg gtcccagtg 
gtccaaatc ccggcgaac agagccaac caagagccc 
cttgttgca ataagacac ccgttccaa aggggtact 
attttggta ccgttgagc caattgcaa ggacgagtg 
gttccagtt gatatcaat gacaatctc agtcaatac 
ggaagacaa aatgaacaa aacactcaa aaaccggat 
gttgagccg cagcctatt tacgtgcgg aggttgcca 
ccgccccag tttagacca attccggtc caagtcgac 
gaaaatgga tatatccga gacttgcaa aggcaacca 
aacggccaa cccattccg taccctgtc ccagtcccg 
ccccaaaaa tacaatgat gtggacaac gatattgac 
gaaatcagc cctcctgtc agtacccga gacttccaa 
aagctcctg caccaatta attttgcgc caaacccgc 
ttggaacgc atcagtgct ttgactgac ccgagacgc 
cacccagaa ttataccaa ccgaggtat aggccagtc 
tatcgccca acacctgtc ccatattac caacaaaac 
ggcccagta caattttta tccgacgat aatgtgcaa 
caaaggcaa agacaatac gtccacatt tcgccagaa 
cagcaagtt gcaattcgt cagcaacaa cgccaagac 
caagaagtg tatcagaac gggcagtct tacattccc 
atgagacga gtggcgagg cttctgcaa ccaggggaa 
gaccaacgt cttgaaaat aaagacgaa tatcttccc 
ccagatgtt agggaaatg ctcttgttg cggatgctc 
caacttgcc ataaaccca gcattacca ctgagcgag 
gatgagatg gtgcaagga acaaccccc gtgccacaa 
tacaagaag gctccagtt cggaatgtg gaaattttg 
ggggaagaa cccgaagag aaacggccc gtgagaagt 
aaaaggtac agagagcca catatggat tattatgaa 
caatag
    mRNA sequence
    • >TC001121 RA
      tgtcttaag ttatatagg gaaccggcg gtttaaatg 
aagcagtcg gtgtaaaca cgtggtgta agcaactgc 
cgcaatggc agatttaac aaagattac tccgttgtt 
tttgttgct ccttctttg caaaaagtc actctgtgc 
agagagtga atcgaagga agtactagt cgagtcaga 
gtcaaagga cggcaagct tgaaataat caaacaaat 
taagaaagt caatgacga tggctccta cactatagg 
ttacgaagc cgatgatgg gtcgttcaa aatcgaaag 
tcgggacgt tttagggaa tattaaggg aacttacgg 
gtatataga cgacgctgg acaaattaa aagagtgtc 
ttacagcac gagcaattc tagtgagat tttaccaaa 
accggatca accatccgt ggttcaaag aatccccaa 
gacaaatcg cacccaaat ttacacttc gtcaaccaa 
acgcccgtt ttattccac aactcagtc gaccccaac 
cagcacaac ggccctcca aagactcgc caaactgcg 
cagtcagtc aacaacaac caccgaaaa accaaacta 
cagcgaagt ggttaacat ggaaagcga aatcaaaac 
cacaagcaa accgcatat agtgtaccc aagctcgac 
acccagaat cttgctaca aggtagatc aacagttag 
atcaaccgc agcaacaca cacccaaac ccaaaaatc 
cgaaggtca gctaattag accagaaat tgtaacagc 
taggccaac agaactgcc attgttcag acgacttgc 
tttgaaaca cccactttt gggtgacga caaacacgt 
ggcggatgg atccgaagt caggagtaa catcttgcg 
gagacaatt gtcccaaga gaaatctga ctttgatcc 
ccaacagca cgttttcac gctacagca agctttagg 
tagcgacgc gactgacgt ttacagtgg ttccgtaac 
cactggaac cccacgacc tttgttcac tactacgtc 
ccgacctag ggctctttt aagtacgac cacaacacc 
aacagtggt ctcgagacc aactatacg gtatccagt 
aaactacca aaggactgt attacaaga cttggaaag 
caacacaca gtatgcgca agaaacaac aaccacgga 
agcaacggt taatgacgc gaccccaac cccggtccc 
agtggtcca aatcccggc gaacagagc caaccaaga 
gccccttgt tgcaataag acacccgtt ccaaagggg 
tactatttt ggtaccgtt gagccaatt gcaaggacg 
agtggttcc agttgatat caatgacaa tctcagtca 
atacggaag acaaaatga acaaaacac tcaaaaacc 
ggatgttga gccgcagcc tatttacgt gcggaggtt 
gccaccgcc ccagtttag accaattcc ggtccaagt 
cgacgaaaa tggatatat ccgagactt gcaaaggca 
accaaacgg ccaacccat tccgtaccc tgtcccagt 
cccgcccca aaaatacaa tgatgtgga caacgatat 
tgacgaaat cagccctcc tgtcagtac ccgagactt 
ccaaaagct cctgcacca attaatttt gcgccaaac 
ccgcttgga acgcatcag tgctttgac tgacccgag 
acgccaccc agaattata ccaaccgag gtataggcc 
agtctatcg cccaacacc tgtcccata ttaccaaca 
aaacggccc agtacaatt tttatccga cgataatgt 
gcaacaaag gcaaagaca atacgtcca catttcgcc 
agaacagca agttgcaat tcgtcagca acaacgcca 
agaccaaga agtgtatca gaacgggca gtcttacat 
tcccatgag acgagtggc gaggcttct gcaaccagg 
ggaagacca acgtcttga aaataaaga cgaatatct 
tcccccaga tgttaggga aatgctctt gttgcggat 
gctccaact tgccataaa cccagcatt accactgag 
cgaggatga gatggtgca aggaacaac ccccgtgcc 
acaatacaa gaaggctcc agttcggaa tgtggaaat 
tttggggga agaacccga agagaaacg gcccgtgag 
aagtaaaag gtacagaga gccacatat ggattatta 
tgaacaata gtagcgctt gttgcgtgt tgtacatac 
aaacggtat actatctta tcttaatga ttacgggac 
aatgacagt tttcgttgc aaaaatgca aagtaggta 
ttaaaatca tcttagttc acagtcatg ttagtacat 
gaggtttat ttattagca tgctcatta tcattacct 
acttttcca atttattta tgttgcaaa tttcgcgga 
cgtcagcgc acctggcgt tgttttggt gatattttt 
gtagatatt tattgttca ttttttact tgttttgta 
ccctagtta ggccacgca taattaata gattgcatg 
cttcaatta tgtaaatat tgcgcaacc attaccaaa 
gatgtgaat actttacgt gggaactta tctacatgt 
cgtttcttt ttaaacaac tgttctatt attacaata 
aaacaatta atgaata
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