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Gene information
General information
TC001120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5772890..5779262
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001120
    iBeetle screen iB_03140
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The head is more often affected than thorax.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: The head is more often affected than thorax.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001120 RA
      MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE 
      QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY 
      GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV 
      QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV 
      VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET 
      EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL 
      FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY 
      VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT 
      PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY 
      TKHLSSPSS TALPSRGTG NPIYSSALV FDPTSGQYN 
      TQLYQTLPQ TEGDISLNQ RIQPYVHQP QRTVVNLQQ 
      LQQQSPLYT HLRPSQAEY QQQQAGLQF QNSAHLYAQ 
      QQRARLQQQ QQQKQAVQQ QPVYYIQPS GGQPLASGQ 
      IDAFLRGHN IQF
    • >TC001120 RB
      MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE 
      QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY 
      GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV 
      QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV 
      VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET 
      EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL 
      FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY 
      VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT 
      PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY 
      TKHLSSPSS TALPSRGSL DFDAEFQKF QQENNIIAP 
      TPTPKTVSK TNKPTTTSA GTGNPIYSS ALVFDPTSG 
      QYNTQLYQT LPQTEGDIS LNQRIQPYV HQPQRTVVN 
      LQQLQQQSP LYTHLRPSQ AEYQQQQAG LQFQNSAHL 
      YAQQQRARL QQQQQQKQA VQQQPVYYI QPSGGQPLA 
      SGQIDAFLR GHNIQF
    CDS sequence
    • >TC001120 RA
      atgcggctt caagcatct tggctgccg ctccttttg 
      tgcggagct gtttttcta agttttacc gaagcccga 
      agaatccgt gtccatgct atccctgaa ccagaggag 
      caagaaacc attcagtat tacgcagct gaaccacaa 
      gatgaagag gttcagtcg ccgcgacaa cgagtagtt 
      ttagtatcg acagctgat cagtacaac ggtctttat 
      ggccaacca actgcttcg tctaggagc agttcagat 
      aattacatt ccttcgtct cgtaaagct cttcccact 
      gctagagcc aaagaggca gcgaaagca cctccagtg 
      caaaccatc agaaattac aacaaagtc aatgatgat 
      ggttcgttc actttcggt tacgaggct gctgatggt 
      agttttaaa gaggagaca agaggtact gactgtgtt 
      gtcagagga aaatacggg tatattgac cccgatggc 
      aacaaaaga gagttcact tatgtttct ggtaatcct 
      tgcgatcca aatgctccg aaagaagaa caagaaacg 
      gaacaagaa cacgaaaac gaagccgat aacggaccg 
      gctaattac ccgactcga ccgatccga ccgatccga 
      cctgtgaca gttgcacca aaaccagcc gtcacctta 
      ttccaaaat aactacgac caaagcgaa gaagaacaa 
      cccgaacct gaacaagtg cttaaacct caacagccg 
      attcgtcgc cccaattac atcactcgc ccaacatac 
      gtccagcaa accgcaact gaagaagcg caagtgtac 
      caaagacca cagctcatt tcagccacc acaccatcg 
      tcctacttg gccaagcag cagagcgtc agcattacc 
      ccacgaccg gtctcacca actccctca gctaggacc 
      cagctcccg gctaccact taccgccca caactactc 
      caagtttct gtaactcca agaccttca gtcttgtac 
      accaaacat ctctcttca ccatcgagc actgcactt 
      ccttcaaga ggtaccgga aacccgatt tactcctca 
      gcgcttgta tttgacccg acctccggt caatacaac 
      acgcagctg taccaaacc cttccgcag acggaaggc 
      gacatctcg ctgaaccag cgcattcaa ccctacgtg 
      catcagcca caaagaact gtggtcaat ttgcagcag 
      cttcagcag cagagcccg ctctacacg catttgagg 
      ccgtctcag gccgagtat cagcaacaa caagccggc 
      ttgcaattc cagaactcg gcgcatttg tacgcccaa 
      cagcaacga gcgagactt caacagcag caacaacag 
      aaacaagcc gtgcagcaa cagcccgtt tactacatc 
      caaccctcg ggggggcag ccgctggcg tcgggtcag 
      attgacgcc ttcctcaga ggccacaat attcagttt 
      taa
    • >TC001120 RB
      atgcggctt caagcatct tggctgccg ctccttttg 
      tgcggagct gtttttcta agttttacc gaagcccga 
      agaatccgt gtccatgct atccctgaa ccagaggag 
      caagaaacc attcagtat tacgcagct gaaccacaa 
      gatgaagag gttcagtcg ccgcgacaa cgagtagtt 
      ttagtatcg acagctgat cagtacaac ggtctttat 
      ggccaacca actgcttcg tctaggagc agttcagat 
      aattacatt ccttcgtct cgtaaagct cttcccact 
      gctagagcc aaagaggca gcgaaagca cctccagtg 
      caaaccatc agaaattac aacaaagtc aatgatgat 
      ggttcgttc actttcggt tacgaggct gctgatggt 
      agttttaaa gaggagaca agaggtact gactgtgtt 
      gtcagagga aaatacggg tatattgac cccgatggc 
      aacaaaaga gagttcact tatgtttct ggtaatcct 
      tgcgatcca aatgctccg aaagaagaa caagaaacg 
      gaacaagaa cacgaaaac gaagccgat aacggaccg 
      gctaattac ccgactcga ccgatccga ccgatccga 
      cctgtgaca gttgcacca aaaccagcc gtcacctta 
      ttccaaaat aactacgac caaagcgaa gaagaacaa 
      cccgaacct gaacaagtg cttaaacct caacagccg 
      attcgtcgc cccaattac atcactcgc ccaacatac 
      gtccagcaa accgcaact gaagaagcg caagtgtac 
      caaagacca cagctcatt tcagccacc acaccatcg 
      tcctacttg gccaagcag cagagcgtc agcattacc 
      ccacgaccg gtctcacca actccctca gctaggacc 
      cagctcccg gctaccact taccgccca caactactc 
      caagtttct gtaactcca agaccttca gtcttgtac 
      accaaacat ctctcttca ccatcgagc actgcactt 
      ccttcaaga ggtagtctc gattttgac gcagaattc 
      caaaaattc cagcaagaa aacaatatt attgcacca 
      acccccact cccaaaact gtctcaaag accaataaa 
      ccaaccact acttctgca ggtaccgga aacccgatt 
      tactcctca gcgcttgta tttgacccg acctccggt 
      caatacaac acgcagctg taccaaacc cttccgcag 
      acggaaggc gacatctcg ctgaaccag cgcattcaa 
      ccctacgtg catcagcca caaagaact gtggtcaat 
      ttgcagcag cttcagcag cagagcccg ctctacacg 
      catttgagg ccgtctcag gccgagtat cagcaacaa 
      caagccggc ttgcaattc cagaactcg gcgcatttg 
      tacgcccaa cagcaacga gcgagactt caacagcag 
      caacaacag aaacaagcc gtgcagcaa cagcccgtt 
      tactacatc caaccctcg ggggggcag ccgctggcg 
      tcgggtcag attgacgcc ttcctcaga ggccacaat 
      attcagttt taa
    mRNA sequence
    • >TC001120 RA
      tatgttata aagacaagt gctcgactc tacctgaag 
      acattatta ctgtttact ctcactgtt gcacggttt 
      cagtggttt cattcacga gaacgtgat tgcgtttac 
      tttcagttc agtgcagtt tggatttag agctttagc 
      agaatgcgg cttcaagca tcttggctg ccgctcctt 
      ttgtgcgga gctgttttt ctaagtttt accgaagcc 
      cgaagaatc cgtgtccat gctatccct gaaccagag 
      gagcaagaa accattcag tattacgca gctgaacca 
      caagatgaa gaggttcag tcgccgcga caacgagta 
      gttttagta tcgacagct gatcagtac aacggtctt 
      tatggccaa ccaactgct tcgtctagg agcagttca 
      gataattac attccttcg tctcgtaaa gctcttccc 
      actgctaga gccaaagag gcagcgaaa gcacctcca 
      gtgcaaacc atcagaaat tacaacaaa gtcaatgat 
      gatggttcg ttcactttc ggttacgag gctgctgat 
      ggtagtttt aaagaggag acaagaggt actgactgt 
      gttgtcaga ggaaaatac gggtatatt gaccccgat 
      ggcaacaaa agagagttc acttatgtt tctggtaat 
      ccttgcgat ccaaatgct ccgaaagaa gaacaagaa 
      acggaacaa gaacacgaa aacgaagcc gataacgga 
      ccggctaat tacccgact cgaccgatc cgaccgatc 
      cgacctgtg acagttgca ccaaaacca gccgtcacc 
      ttattccaa aataactac gaccaaagc gaagaagaa 
      caacccgaa cctgaacaa gtgcttaaa cctcaacag 
      ccgattcgt cgccccaat tacatcact cgcccaaca 
      tacgtccag caaaccgca actgaagaa gcgcaagtg 
      taccaaaga ccacagctc atttcagcc accacacca 
      tcgtcctac ttggccaag cagcagagc gtcagcatt 
      accccacga ccggtctca ccaactccc tcagctagg 
      acccagctc ccggctacc acttaccgc ccacaacta 
      ctccaagtt tctgtaact ccaagacct tcagtcttg 
      tacaccaaa catctctct tcaccatcg agcactgca 
      cttccttca agaggtacc ggaaacccg atttactcc 
      tcagcgctt gtatttgac ccgacctcc ggtcaatac 
      aacacgcag ctgtaccaa acccttccg cagacggaa 
      ggcgacatc tcgctgaac cagcgcatt caaccctac 
      gtgcatcag ccacaaaga actgtggtc aatttgcag 
      cagcttcag cagcagagc ccgctctac acgcatttg 
      aggccgtct caggccgag tatcagcaa caacaagcc 
      ggcttgcaa ttccagaac tcggcgcat ttgtacgcc 
      caacagcaa cgagcgaga cttcaacag cagcaacaa 
      cagaaacaa gccgtgcag caacagccc gtttactac 
      atccaaccc tcggggggg cagccgctg gcgtcgggt 
      cagattgac gccttcctc agaggccac aatattcag 
      ttttaagtg tttcgagag actagaatc tattagact 
      gatccatgc agagggtgt aggtaacct ctggctgtc 
      cttttagtg aaagtgcaa cgatatcca ccataaaca 
      cggtcacgc cccgattag gtaactgcg tttgcatgc 
      tcgaatttt taaagcatc gccaagtgt tacttttca 
      cttttgcat ctgcataac tttcgacat tgtcttgat 
      tcattgtca ttcctttca aggccgctg ggttgcctt 
      ggatattgt aggtattta tggttcgat tttgtgttg 
      ttgtgcctt atgtaatga aaattacca aatgctaat 
      atggaaatg agcagtcgt tattgcgcg gttttgtac 
      ataacgcag atataaagc tctagtttt aactcttga 
      tgtaataat atagtatta ttattataa tgtttgtat 
      tttgttatg taagaccat taattgaaa taaatattg 
      tatatattt
    • >TC001120 RB
      tatgttata aagacaagt gctcgactc tacctgaag 
      acattatta ctgtttact ctcactgtt gcacggttt 
      cagtggttt cattcacga gaacgtgat tgcgtttac 
      tttcagttc agtgcagtt tggatttag agctttagc 
      agaatgcgg cttcaagca tcttggctg ccgctcctt 
      ttgtgcgga gctgttttt ctaagtttt accgaagcc 
      cgaagaatc cgtgtccat gctatccct gaaccagag 
      gagcaagaa accattcag tattacgca gctgaacca 
      caagatgaa gaggttcag tcgccgcga caacgagta 
      gttttagta tcgacagct gatcagtac aacggtctt 
      tatggccaa ccaactgct tcgtctagg agcagttca 
      gataattac attccttcg tctcgtaaa gctcttccc 
      actgctaga gccaaagag gcagcgaaa gcacctcca 
      gtgcaaacc atcagaaat tacaacaaa gtcaatgat 
      gatggttcg ttcactttc ggttacgag gctgctgat 
      ggtagtttt aaagaggag acaagaggt actgactgt 
      gttgtcaga ggaaaatac gggtatatt gaccccgat 
      ggcaacaaa agagagttc acttatgtt tctggtaat 
      ccttgcgat ccaaatgct ccgaaagaa gaacaagaa 
      acggaacaa gaacacgaa aacgaagcc gataacgga 
      ccggctaat tacccgact cgaccgatc cgaccgatc 
      cgacctgtg acagttgca ccaaaacca gccgtcacc 
      ttattccaa aataactac gaccaaagc gaagaagaa 
      caacccgaa cctgaacaa gtgcttaaa cctcaacag 
      ccgattcgt cgccccaat tacatcact cgcccaaca 
      tacgtccag caaaccgca actgaagaa gcgcaagtg 
      taccaaaga ccacagctc atttcagcc accacacca 
      tcgtcctac ttggccaag cagcagagc gtcagcatt 
      accccacga ccggtctca ccaactccc tcagctagg 
      acccagctc ccggctacc acttaccgc ccacaacta 
      ctccaagtt tctgtaact ccaagacct tcagtcttg 
      tacaccaaa catctctct tcaccatcg agcactgca 
      cttccttca agaggtagt ctcgatttt gacgcagaa 
      ttccaaaaa ttccagcaa gaaaacaat attattgca 
      ccaaccccc actcccaaa actgtctca aagaccaat 
      aaaccaacc actacttct gcaggtacc ggaaacccg 
      atttactcc tcagcgctt gtatttgac ccgacctcc 
      ggtcaatac aacacgcag ctgtaccaa acccttccg 
      cagacggaa ggcgacatc tcgctgaac cagcgcatt 
      caaccctac gtgcatcag ccacaaaga actgtggtc 
      aatttgcag cagcttcag cagcagagc ccgctctac 
      acgcatttg aggccgtct caggccgag tatcagcaa 
      caacaagcc ggcttgcaa ttccagaac tcggcgcat 
      ttgtacgcc caacagcaa cgagcgaga cttcaacag 
      cagcaacaa cagaaacaa gccgtgcag caacagccc 
      gtttactac atccaaccc tcggggggg cagccgctg 
      gcgtcgggt cagattgac gccttcctc agaggccac 
      aatattcag ttttaagtg tttcgagag actagaatc 
      tattagact gatccatgc agagggtgt aggtaacct 
      ctggctgtc cttttagtg aaagtgcaa cgatatcca 
      ccataaaca cggtcacgc cccgattag gtaactgcg 
      tttgcatgc tcgaatttt taaagcatc gccaagtgt 
      tacttttca cttttgcat ctgcataac tttcgacat 
      tgtcttgat tcattgtca ttcctttca aggccgctg 
      ggttgcctt ggatattgt aggtattta tggttcgat 
      tttgtgttg ttgtgcctt atgtaatga aaattacca 
      aatgctaat atggaaatg agcagtcgt tattgcgcg 
      gttttgtac ataacgcag atataaagc tctagtttt 
      aactcttga tgtaataat atagtatta ttattataa 
      tgtttgtat tttgttatg taagaccat taattgaaa 
      taaatattg tatatattt