| General information | |||
| TC001120 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11279433..11285805 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001120 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03140 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: The head is more often affected than thorax. |
| Comment: The head is more often affected than thorax. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001120 RA
MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY TKHLSSPSS TALPSRGTG NPIYSSALV FDPTSGQYN TQLYQTLPQ TEGDISLNQ RIQPYVHQP QRTVVNLQQ LQQQSPLYT HLRPSQAEY QQQQAGLQF QNSAHLYAQ QQRARLQQQ QQQKQAVQQ QPVYYIQPS GGQPLASGQ IDAFLRGHN IQF
- >TC001120 RB
MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY TKHLSSPSS TALPSRGSL DFDAEFQKF QQENNIIAP TPTPKTVSK TNKPTTTSA GTGNPIYSS ALVFDPTSG QYNTQLYQT LPQTEGDIS LNQRIQPYV HQPQRTVVN LQQLQQQSP LYTHLRPSQ AEYQQQQAG LQFQNSAHL YAQQQRARL QQQQQQKQA VQQQPVYYI QPSGGQPLA SGQIDAFLR GHNIQF
- >TC001120 RA
atgcggctt caagcatct tggctgccg ctccttttg tgcggagct gtttttcta agttttacc gaagcccga agaatccgt gtccatgct atccctgaa ccagaggag caagaaacc attcagtat tacgcagct gaaccacaa gatgaagag gttcagtcg ccgcgacaa cgagtagtt ttagtatcg acagctgat cagtacaac ggtctttat ggccaacca actgcttcg tctaggagc agttcagat aattacatt ccttcgtct cgtaaagct cttcccact gctagagcc aaagaggca gcgaaagca cctccagtg caaaccatc agaaattac aacaaagtc aatgatgat ggttcgttc actttcggt tacgaggct gctgatggt agttttaaa gaggagaca agaggtact gactgtgtt gtcagagga aaatacggg tatattgac cccgatggc aacaaaaga gagttcact tatgtttct ggtaatcct tgcgatcca aatgctccg aaagaagaa caagaaacg gaacaagaa cacgaaaac gaagccgat aacggaccg gctaattac ccgactcga ccgatccga ccgatccga cctgtgaca gttgcacca aaaccagcc gtcacctta ttccaaaat aactacgac caaagcgaa gaagaacaa cccgaacct gaacaagtg cttaaacct caacagccg attcgtcgc cccaattac atcactcgc ccaacatac gtccagcaa accgcaact gaagaagcg caagtgtac caaagacca cagctcatt tcagccacc acaccatcg tcctacttg gccaagcag cagagcgtc agcattacc ccacgaccg gtctcacca actccctca gctaggacc cagctcccg gctaccact taccgccca caactactc caagtttct gtaactcca agaccttca gtcttgtac accaaacat ctctcttca ccatcgagc actgcactt ccttcaaga ggtaccgga aacccgatt tactcctca gcgcttgta tttgacccg acctccggt caatacaac acgcagctg taccaaacc cttccgcag acggaaggc gacatctcg ctgaaccag cgcattcaa ccctacgtg catcagcca caaagaact gtggtcaat ttgcagcag cttcagcag cagagcccg ctctacacg catttgagg ccgtctcag gccgagtat cagcaacaa caagccggc ttgcaattc cagaactcg gcgcatttg tacgcccaa cagcaacga gcgagactt caacagcag caacaacag aaacaagcc gtgcagcaa cagcccgtt tactacatc caaccctcg ggggggcag ccgctggcg tcgggtcag attgacgcc ttcctcaga ggccacaat attcagttt taa
- >TC001120 RB
atgcggctt caagcatct tggctgccg ctccttttg tgcggagct gtttttcta agttttacc gaagcccga agaatccgt gtccatgct atccctgaa ccagaggag caagaaacc attcagtat tacgcagct gaaccacaa gatgaagag gttcagtcg ccgcgacaa cgagtagtt ttagtatcg acagctgat cagtacaac ggtctttat ggccaacca actgcttcg tctaggagc agttcagat aattacatt ccttcgtct cgtaaagct cttcccact gctagagcc aaagaggca gcgaaagca cctccagtg caaaccatc agaaattac aacaaagtc aatgatgat ggttcgttc actttcggt tacgaggct gctgatggt agttttaaa gaggagaca agaggtact gactgtgtt gtcagagga aaatacggg tatattgac cccgatggc aacaaaaga gagttcact tatgtttct ggtaatcct tgcgatcca aatgctccg aaagaagaa caagaaacg gaacaagaa cacgaaaac gaagccgat aacggaccg gctaattac ccgactcga ccgatccga ccgatccga cctgtgaca gttgcacca aaaccagcc gtcacctta ttccaaaat aactacgac caaagcgaa gaagaacaa cccgaacct gaacaagtg cttaaacct caacagccg attcgtcgc cccaattac atcactcgc ccaacatac gtccagcaa accgcaact gaagaagcg caagtgtac caaagacca cagctcatt tcagccacc acaccatcg tcctacttg gccaagcag cagagcgtc agcattacc ccacgaccg gtctcacca actccctca gctaggacc cagctcccg gctaccact taccgccca caactactc caagtttct gtaactcca agaccttca gtcttgtac accaaacat ctctcttca ccatcgagc actgcactt ccttcaaga ggtagtctc gattttgac gcagaattc caaaaattc cagcaagaa aacaatatt attgcacca acccccact cccaaaact gtctcaaag accaataaa ccaaccact acttctgca ggtaccgga aacccgatt tactcctca gcgcttgta tttgacccg acctccggt caatacaac acgcagctg taccaaacc cttccgcag acggaaggc gacatctcg ctgaaccag cgcattcaa ccctacgtg catcagcca caaagaact gtggtcaat ttgcagcag cttcagcag cagagcccg ctctacacg catttgagg ccgtctcag gccgagtat cagcaacaa caagccggc ttgcaattc cagaactcg gcgcatttg tacgcccaa cagcaacga gcgagactt caacagcag caacaacag aaacaagcc gtgcagcaa cagcccgtt tactacatc caaccctcg ggggggcag ccgctggcg tcgggtcag attgacgcc ttcctcaga ggccacaat attcagttt taa
- >TC001120 RA
tatgttata aagacaagt gctcgactc tacctgaag acattatta ctgtttact ctcactgtt gcacggttt cagtggttt cattcacga gaacgtgat tgcgtttac tttcagttc agtgcagtt tggatttag agctttagc agaatgcgg cttcaagca tcttggctg ccgctcctt ttgtgcgga gctgttttt ctaagtttt accgaagcc cgaagaatc cgtgtccat gctatccct gaaccagag gagcaagaa accattcag tattacgca gctgaacca caagatgaa gaggttcag tcgccgcga caacgagta gttttagta tcgacagct gatcagtac aacggtctt tatggccaa ccaactgct tcgtctagg agcagttca gataattac attccttcg tctcgtaaa gctcttccc actgctaga gccaaagag gcagcgaaa gcacctcca gtgcaaacc atcagaaat tacaacaaa gtcaatgat gatggttcg ttcactttc ggttacgag gctgctgat ggtagtttt aaagaggag acaagaggt actgactgt gttgtcaga ggaaaatac gggtatatt gaccccgat ggcaacaaa agagagttc acttatgtt tctggtaat ccttgcgat ccaaatgct ccgaaagaa gaacaagaa acggaacaa gaacacgaa aacgaagcc gataacgga ccggctaat tacccgact cgaccgatc cgaccgatc cgacctgtg acagttgca ccaaaacca gccgtcacc ttattccaa aataactac gaccaaagc gaagaagaa caacccgaa cctgaacaa gtgcttaaa cctcaacag ccgattcgt cgccccaat tacatcact cgcccaaca tacgtccag caaaccgca actgaagaa gcgcaagtg taccaaaga ccacagctc atttcagcc accacacca tcgtcctac ttggccaag cagcagagc gtcagcatt accccacga ccggtctca ccaactccc tcagctagg acccagctc ccggctacc acttaccgc ccacaacta ctccaagtt tctgtaact ccaagacct tcagtcttg tacaccaaa catctctct tcaccatcg agcactgca cttccttca agaggtacc ggaaacccg atttactcc tcagcgctt gtatttgac ccgacctcc ggtcaatac aacacgcag ctgtaccaa acccttccg cagacggaa ggcgacatc tcgctgaac cagcgcatt caaccctac gtgcatcag ccacaaaga actgtggtc aatttgcag cagcttcag cagcagagc ccgctctac acgcatttg aggccgtct caggccgag tatcagcaa caacaagcc ggcttgcaa ttccagaac tcggcgcat ttgtacgcc caacagcaa cgagcgaga cttcaacag cagcaacaa cagaaacaa gccgtgcag caacagccc gtttactac atccaaccc tcggggggg cagccgctg gcgtcgggt cagattgac gccttcctc agaggccac aatattcag ttttaagtg tttcgagag actagaatc tattagact gatccatgc agagggtgt aggtaacct ctggctgtc cttttagtg aaagtgcaa cgatatcca ccataaaca cggtcacgc cccgattag gtaactgcg tttgcatgc tcgaatttt taaagcatc gccaagtgt tacttttca cttttgcat ctgcataac tttcgacat tgtcttgat tcattgtca ttcctttca aggccgctg ggttgcctt ggatattgt aggtattta tggttcgat tttgtgttg ttgtgcctt atgtaatga aaattacca aatgctaat atggaaatg agcagtcgt tattgcgcg gttttgtac ataacgcag atataaagc tctagtttt aactcttga tgtaataat atagtatta ttattataa tgtttgtat tttgttatg taagaccat taattgaaa taaatattg tatatattt
- >TC001120 RB
tatgttata aagacaagt gctcgactc tacctgaag acattatta ctgtttact ctcactgtt gcacggttt cagtggttt cattcacga gaacgtgat tgcgtttac tttcagttc agtgcagtt tggatttag agctttagc agaatgcgg cttcaagca tcttggctg ccgctcctt ttgtgcgga gctgttttt ctaagtttt accgaagcc cgaagaatc cgtgtccat gctatccct gaaccagag gagcaagaa accattcag tattacgca gctgaacca caagatgaa gaggttcag tcgccgcga caacgagta gttttagta tcgacagct gatcagtac aacggtctt tatggccaa ccaactgct tcgtctagg agcagttca gataattac attccttcg tctcgtaaa gctcttccc actgctaga gccaaagag gcagcgaaa gcacctcca gtgcaaacc atcagaaat tacaacaaa gtcaatgat gatggttcg ttcactttc ggttacgag gctgctgat ggtagtttt aaagaggag acaagaggt actgactgt gttgtcaga ggaaaatac gggtatatt gaccccgat ggcaacaaa agagagttc acttatgtt tctggtaat ccttgcgat ccaaatgct ccgaaagaa gaacaagaa acggaacaa gaacacgaa aacgaagcc gataacgga ccggctaat tacccgact cgaccgatc cgaccgatc cgacctgtg acagttgca ccaaaacca gccgtcacc ttattccaa aataactac gaccaaagc gaagaagaa caacccgaa cctgaacaa gtgcttaaa cctcaacag ccgattcgt cgccccaat tacatcact cgcccaaca tacgtccag caaaccgca actgaagaa gcgcaagtg taccaaaga ccacagctc atttcagcc accacacca tcgtcctac ttggccaag cagcagagc gtcagcatt accccacga ccggtctca ccaactccc tcagctagg acccagctc ccggctacc acttaccgc ccacaacta ctccaagtt tctgtaact ccaagacct tcagtcttg tacaccaaa catctctct tcaccatcg agcactgca cttccttca agaggtagt ctcgatttt gacgcagaa ttccaaaaa ttccagcaa gaaaacaat attattgca ccaaccccc actcccaaa actgtctca aagaccaat aaaccaacc actacttct gcaggtacc ggaaacccg atttactcc tcagcgctt gtatttgac ccgacctcc ggtcaatac aacacgcag ctgtaccaa acccttccg cagacggaa ggcgacatc tcgctgaac cagcgcatt caaccctac gtgcatcag ccacaaaga actgtggtc aatttgcag cagcttcag cagcagagc ccgctctac acgcatttg aggccgtct caggccgag tatcagcaa caacaagcc ggcttgcaa ttccagaac tcggcgcat ttgtacgcc caacagcaa cgagcgaga cttcaacag cagcaacaa cagaaacaa gccgtgcag caacagccc gtttactac atccaaccc tcggggggg cagccgctg gcgtcgggt cagattgac gccttcctc agaggccac aatattcag ttttaagtg tttcgagag actagaatc tattagact gatccatgc agagggtgt aggtaacct ctggctgtc cttttagtg aaagtgcaa cgatatcca ccataaaca cggtcacgc cccgattag gtaactgcg tttgcatgc tcgaatttt taaagcatc gccaagtgt tacttttca cttttgcat ctgcataac tttcgacat tgtcttgat tcattgtca ttcctttca aggccgctg ggttgcctt ggatattgt aggtattta tggttcgat tttgtgttg ttgtgcctt atgtaatga aaattacca aatgctaat atggaaatg agcagtcgt tattgcgcg gttttgtac ataacgcag atataaagc tctagtttt aactcttga tgtaataat atagtatta ttattataa tgtttgtat tttgttatg taagaccat taattgaaa taaatattg tatatattt
