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Gene information
General information
TC001120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5772890..5779262
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001120
    iBeetle screen iB_03140
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The head is more often affected than thorax.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: The head is more often affected than thorax.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001120 RA
      MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE 
QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY 
GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV 
QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV 
VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET 
EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL 
FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY 
VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT 
PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY 
TKHLSSPSS TALPSRGTG NPIYSSALV FDPTSGQYN 
TQLYQTLPQ TEGDISLNQ RIQPYVHQP QRTVVNLQQ 
LQQQSPLYT HLRPSQAEY QQQQAGLQF QNSAHLYAQ 
QQRARLQQQ QQQKQAVQQ QPVYYIQPS GGQPLASGQ 
IDAFLRGHN IQF
    • >TC001120 RB
      MRLQASWLP LLLCGAVFL SFTEARRIR VHAIPEPEE 
QETIQYYAA EPQDEEVQS PRQRVVLVS TADQYNGLY 
GQPTASSRS SSDNYIPSS RKALPTARA KEAAKAPPV 
QTIRNYNKV NDDGSFTFG YEAADGSFK EETRGTDCV 
VRGKYGYID PDGNKREFT YVSGNPCDP NAPKEEQET 
EQEHENEAD NGPANYPTR PIRPIRPVT VAPKPAVTL 
FQNNYDQSE EEQPEPEQV LKPQQPIRR PNYITRPTY 
VQQTATEEA QVYQRPQLI SATTPSSYL AKQQSVSIT 
PRPVSPTPS ARTQLPATT YRPQLLQVS VTPRPSVLY 
TKHLSSPSS TALPSRGSL DFDAEFQKF QQENNIIAP 
TPTPKTVSK TNKPTTTSA GTGNPIYSS ALVFDPTSG 
QYNTQLYQT LPQTEGDIS LNQRIQPYV HQPQRTVVN 
LQQLQQQSP LYTHLRPSQ AEYQQQQAG LQFQNSAHL 
YAQQQRARL QQQQQQKQA VQQQPVYYI QPSGGQPLA 
SGQIDAFLR GHNIQF
    CDS sequence
    • >TC001120 RA
      atgcggctt caagcatct tggctgccg ctccttttg 
tgcggagct gtttttcta agttttacc gaagcccga 
agaatccgt gtccatgct atccctgaa ccagaggag 
caagaaacc attcagtat tacgcagct gaaccacaa 
gatgaagag gttcagtcg ccgcgacaa cgagtagtt 
ttagtatcg acagctgat cagtacaac ggtctttat 
ggccaacca actgcttcg tctaggagc agttcagat 
aattacatt ccttcgtct cgtaaagct cttcccact 
gctagagcc aaagaggca gcgaaagca cctccagtg 
caaaccatc agaaattac aacaaagtc aatgatgat 
ggttcgttc actttcggt tacgaggct gctgatggt 
agttttaaa gaggagaca agaggtact gactgtgtt 
gtcagagga aaatacggg tatattgac cccgatggc 
aacaaaaga gagttcact tatgtttct ggtaatcct 
tgcgatcca aatgctccg aaagaagaa caagaaacg 
gaacaagaa cacgaaaac gaagccgat aacggaccg 
gctaattac ccgactcga ccgatccga ccgatccga 
cctgtgaca gttgcacca aaaccagcc gtcacctta 
ttccaaaat aactacgac caaagcgaa gaagaacaa 
cccgaacct gaacaagtg cttaaacct caacagccg 
attcgtcgc cccaattac atcactcgc ccaacatac 
gtccagcaa accgcaact gaagaagcg caagtgtac 
caaagacca cagctcatt tcagccacc acaccatcg 
tcctacttg gccaagcag cagagcgtc agcattacc 
ccacgaccg gtctcacca actccctca gctaggacc 
cagctcccg gctaccact taccgccca caactactc 
caagtttct gtaactcca agaccttca gtcttgtac 
accaaacat ctctcttca ccatcgagc actgcactt 
ccttcaaga ggtaccgga aacccgatt tactcctca 
gcgcttgta tttgacccg acctccggt caatacaac 
acgcagctg taccaaacc cttccgcag acggaaggc 
gacatctcg ctgaaccag cgcattcaa ccctacgtg 
catcagcca caaagaact gtggtcaat ttgcagcag 
cttcagcag cagagcccg ctctacacg catttgagg 
ccgtctcag gccgagtat cagcaacaa caagccggc 
ttgcaattc cagaactcg gcgcatttg tacgcccaa 
cagcaacga gcgagactt caacagcag caacaacag 
aaacaagcc gtgcagcaa cagcccgtt tactacatc 
caaccctcg ggggggcag ccgctggcg tcgggtcag 
attgacgcc ttcctcaga ggccacaat attcagttt 
taa
    • >TC001120 RB
      atgcggctt caagcatct tggctgccg ctccttttg 
tgcggagct gtttttcta agttttacc gaagcccga 
agaatccgt gtccatgct atccctgaa ccagaggag 
caagaaacc attcagtat tacgcagct gaaccacaa 
gatgaagag gttcagtcg ccgcgacaa cgagtagtt 
ttagtatcg acagctgat cagtacaac ggtctttat 
ggccaacca actgcttcg tctaggagc agttcagat 
aattacatt ccttcgtct cgtaaagct cttcccact 
gctagagcc aaagaggca gcgaaagca cctccagtg 
caaaccatc agaaattac aacaaagtc aatgatgat 
ggttcgttc actttcggt tacgaggct gctgatggt 
agttttaaa gaggagaca agaggtact gactgtgtt 
gtcagagga aaatacggg tatattgac cccgatggc 
aacaaaaga gagttcact tatgtttct ggtaatcct 
tgcgatcca aatgctccg aaagaagaa caagaaacg 
gaacaagaa cacgaaaac gaagccgat aacggaccg 
gctaattac ccgactcga ccgatccga ccgatccga 
cctgtgaca gttgcacca aaaccagcc gtcacctta 
ttccaaaat aactacgac caaagcgaa gaagaacaa 
cccgaacct gaacaagtg cttaaacct caacagccg 
attcgtcgc cccaattac atcactcgc ccaacatac 
gtccagcaa accgcaact gaagaagcg caagtgtac 
caaagacca cagctcatt tcagccacc acaccatcg 
tcctacttg gccaagcag cagagcgtc agcattacc 
ccacgaccg gtctcacca actccctca gctaggacc 
cagctcccg gctaccact taccgccca caactactc 
caagtttct gtaactcca agaccttca gtcttgtac 
accaaacat ctctcttca ccatcgagc actgcactt 
ccttcaaga ggtagtctc gattttgac gcagaattc 
caaaaattc cagcaagaa aacaatatt attgcacca 
acccccact cccaaaact gtctcaaag accaataaa 
ccaaccact acttctgca ggtaccgga aacccgatt 
tactcctca gcgcttgta tttgacccg acctccggt 
caatacaac acgcagctg taccaaacc cttccgcag 
acggaaggc gacatctcg ctgaaccag cgcattcaa 
ccctacgtg catcagcca caaagaact gtggtcaat 
ttgcagcag cttcagcag cagagcccg ctctacacg 
catttgagg ccgtctcag gccgagtat cagcaacaa 
caagccggc ttgcaattc cagaactcg gcgcatttg 
tacgcccaa cagcaacga gcgagactt caacagcag 
caacaacag aaacaagcc gtgcagcaa cagcccgtt 
tactacatc caaccctcg ggggggcag ccgctggcg 
tcgggtcag attgacgcc ttcctcaga ggccacaat 
attcagttt taa
    mRNA sequence
    • >TC001120 RA
      tatgttata aagacaagt gctcgactc tacctgaag 
acattatta ctgtttact ctcactgtt gcacggttt 
cagtggttt cattcacga gaacgtgat tgcgtttac 
tttcagttc agtgcagtt tggatttag agctttagc 
agaatgcgg cttcaagca tcttggctg ccgctcctt 
ttgtgcgga gctgttttt ctaagtttt accgaagcc 
cgaagaatc cgtgtccat gctatccct gaaccagag 
gagcaagaa accattcag tattacgca gctgaacca 
caagatgaa gaggttcag tcgccgcga caacgagta 
gttttagta tcgacagct gatcagtac aacggtctt 
tatggccaa ccaactgct tcgtctagg agcagttca 
gataattac attccttcg tctcgtaaa gctcttccc 
actgctaga gccaaagag gcagcgaaa gcacctcca 
gtgcaaacc atcagaaat tacaacaaa gtcaatgat 
gatggttcg ttcactttc ggttacgag gctgctgat 
ggtagtttt aaagaggag acaagaggt actgactgt 
gttgtcaga ggaaaatac gggtatatt gaccccgat 
ggcaacaaa agagagttc acttatgtt tctggtaat 
ccttgcgat ccaaatgct ccgaaagaa gaacaagaa 
acggaacaa gaacacgaa aacgaagcc gataacgga 
ccggctaat tacccgact cgaccgatc cgaccgatc 
cgacctgtg acagttgca ccaaaacca gccgtcacc 
ttattccaa aataactac gaccaaagc gaagaagaa 
caacccgaa cctgaacaa gtgcttaaa cctcaacag 
ccgattcgt cgccccaat tacatcact cgcccaaca 
tacgtccag caaaccgca actgaagaa gcgcaagtg 
taccaaaga ccacagctc atttcagcc accacacca 
tcgtcctac ttggccaag cagcagagc gtcagcatt 
accccacga ccggtctca ccaactccc tcagctagg 
acccagctc ccggctacc acttaccgc ccacaacta 
ctccaagtt tctgtaact ccaagacct tcagtcttg 
tacaccaaa catctctct tcaccatcg agcactgca 
cttccttca agaggtacc ggaaacccg atttactcc 
tcagcgctt gtatttgac ccgacctcc ggtcaatac 
aacacgcag ctgtaccaa acccttccg cagacggaa 
ggcgacatc tcgctgaac cagcgcatt caaccctac 
gtgcatcag ccacaaaga actgtggtc aatttgcag 
cagcttcag cagcagagc ccgctctac acgcatttg 
aggccgtct caggccgag tatcagcaa caacaagcc 
ggcttgcaa ttccagaac tcggcgcat ttgtacgcc 
caacagcaa cgagcgaga cttcaacag cagcaacaa 
cagaaacaa gccgtgcag caacagccc gtttactac 
atccaaccc tcggggggg cagccgctg gcgtcgggt 
cagattgac gccttcctc agaggccac aatattcag 
ttttaagtg tttcgagag actagaatc tattagact 
gatccatgc agagggtgt aggtaacct ctggctgtc 
cttttagtg aaagtgcaa cgatatcca ccataaaca 
cggtcacgc cccgattag gtaactgcg tttgcatgc 
tcgaatttt taaagcatc gccaagtgt tacttttca 
cttttgcat ctgcataac tttcgacat tgtcttgat 
tcattgtca ttcctttca aggccgctg ggttgcctt 
ggatattgt aggtattta tggttcgat tttgtgttg 
ttgtgcctt atgtaatga aaattacca aatgctaat 
atggaaatg agcagtcgt tattgcgcg gttttgtac 
ataacgcag atataaagc tctagtttt aactcttga 
tgtaataat atagtatta ttattataa tgtttgtat 
tttgttatg taagaccat taattgaaa taaatattg 
tatatattt
    • >TC001120 RB
      tatgttata aagacaagt gctcgactc tacctgaag 
acattatta ctgtttact ctcactgtt gcacggttt 
cagtggttt cattcacga gaacgtgat tgcgtttac 
tttcagttc agtgcagtt tggatttag agctttagc 
agaatgcgg cttcaagca tcttggctg ccgctcctt 
ttgtgcgga gctgttttt ctaagtttt accgaagcc 
cgaagaatc cgtgtccat gctatccct gaaccagag 
gagcaagaa accattcag tattacgca gctgaacca 
caagatgaa gaggttcag tcgccgcga caacgagta 
gttttagta tcgacagct gatcagtac aacggtctt 
tatggccaa ccaactgct tcgtctagg agcagttca 
gataattac attccttcg tctcgtaaa gctcttccc 
actgctaga gccaaagag gcagcgaaa gcacctcca 
gtgcaaacc atcagaaat tacaacaaa gtcaatgat 
gatggttcg ttcactttc ggttacgag gctgctgat 
ggtagtttt aaagaggag acaagaggt actgactgt 
gttgtcaga ggaaaatac gggtatatt gaccccgat 
ggcaacaaa agagagttc acttatgtt tctggtaat 
ccttgcgat ccaaatgct ccgaaagaa gaacaagaa 
acggaacaa gaacacgaa aacgaagcc gataacgga 
ccggctaat tacccgact cgaccgatc cgaccgatc 
cgacctgtg acagttgca ccaaaacca gccgtcacc 
ttattccaa aataactac gaccaaagc gaagaagaa 
caacccgaa cctgaacaa gtgcttaaa cctcaacag 
ccgattcgt cgccccaat tacatcact cgcccaaca 
tacgtccag caaaccgca actgaagaa gcgcaagtg 
taccaaaga ccacagctc atttcagcc accacacca 
tcgtcctac ttggccaag cagcagagc gtcagcatt 
accccacga ccggtctca ccaactccc tcagctagg 
acccagctc ccggctacc acttaccgc ccacaacta 
ctccaagtt tctgtaact ccaagacct tcagtcttg 
tacaccaaa catctctct tcaccatcg agcactgca 
cttccttca agaggtagt ctcgatttt gacgcagaa 
ttccaaaaa ttccagcaa gaaaacaat attattgca 
ccaaccccc actcccaaa actgtctca aagaccaat 
aaaccaacc actacttct gcaggtacc ggaaacccg 
atttactcc tcagcgctt gtatttgac ccgacctcc 
ggtcaatac aacacgcag ctgtaccaa acccttccg 
cagacggaa ggcgacatc tcgctgaac cagcgcatt 
caaccctac gtgcatcag ccacaaaga actgtggtc 
aatttgcag cagcttcag cagcagagc ccgctctac 
acgcatttg aggccgtct caggccgag tatcagcaa 
caacaagcc ggcttgcaa ttccagaac tcggcgcat 
ttgtacgcc caacagcaa cgagcgaga cttcaacag 
cagcaacaa cagaaacaa gccgtgcag caacagccc 
gtttactac atccaaccc tcggggggg cagccgctg 
gcgtcgggt cagattgac gccttcctc agaggccac 
aatattcag ttttaagtg tttcgagag actagaatc 
tattagact gatccatgc agagggtgt aggtaacct 
ctggctgtc cttttagtg aaagtgcaa cgatatcca 
ccataaaca cggtcacgc cccgattag gtaactgcg 
tttgcatgc tcgaatttt taaagcatc gccaagtgt 
tacttttca cttttgcat ctgcataac tttcgacat 
tgtcttgat tcattgtca ttcctttca aggccgctg 
ggttgcctt ggatattgt aggtattta tggttcgat 
tttgtgttg ttgtgcctt atgtaatga aaattacca 
aatgctaat atggaaatg agcagtcgt tattgcgcg 
gttttgtac ataacgcag atataaagc tctagtttt 
aactcttga tgtaataat atagtatta ttattataa 
tgtttgtat tttgttatg taagaccat taattgaaa 
taaatattg tatatattt