| General information | |||
| TC001109 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11077462..11090981 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001109 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03138 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001109 RA
MTQRDEVQK FEQGRIRVL QEERLYIQK KTFTKWMNS FLQKVRMEV DDLFVDLAD GKKLLKLLE IISGEKLAK PNNGKMRVH KIENVNKSL AFLHTKVRL ESIGAEDIV DGNPRLILG LIWTIILRF QIQEIEIDV DEENESSEK KSAKDALLL WAQRKTQGY KGVDIRDFS TSWRTGLGF NALIHAHRP DLFTYDDLV HNRNIDNLN HAFDVANNE LGIPRLLDA EDIDNSRPD EKSIMTYVA SYYHTFARM KNEEKSGRR IAKIIKQMV DADKMKVNY DELTTDLLN WIKIKIVEL EDRNFPNSL EGIQTLFLA FARYRTKEK PPKYIERSE IEALFFNIN TQLKELRQP AFIPPDGKL VQDIERAWE TLERAEHNR EVALRNELL RQERLEQLY CKFERKSVL REGYVKEMI QVLSDPRYG SNRDQVDAT VKKHEAISA DILARQERV NDLTQMCNE LVREKYRNS DRVVKREAE ILNQWKQLL DLLEKHKLK LNRMVNIKS LEMEIDTTL NSMQQLKAD LSTTDTGIH LMAVEELLH KHALQELQV SSLGETERR LNRLAESTL AQNPQEEKL KKKIQELSA TYKDLQDAS DKRKGLLEE ARNFYQFLQ DQEDEEAWL IEKQRICQA GITAKDLRG VLSLQQKHK VLMDEIKAR RNKFDQLGA TGRQLIAEK HPRSNEIQQ HMDRNKQAW SDLEKLVND RTKQLQDAA ETYQFYADA NEADSWLNE KTSILNSSD YGSDEPSAQ ALLQRHKDL EGELNAYKG DILSLNTQA DRLIASGIS NLDLTAEVE VAEPVEEIV YENRMIPTE VWVEEPVEK VEYKMVVEE KKVPQVKAL YPFNDHGLN MVKGEVMYL LNKSNPDWW CVRKADGTD GFAPAKYVT EIEPRLLQM QYRKPETVK VMQKVKKTK MVNQKVPVK VFRQRKPVK RKVDDNDSV PKRQKKIND TYQHLLDVA AKRHALLED AVKLFRFYK ECDDFEKWI KDKEKLLAV DDPNDSIEQ AKRKYEKFV TDLSANNKR MDELNDDVK EFEKQKHSQ IDKIKSRHR QVQGAWQRL NLLKARKEK SLEGASSVE LFQKTCDEA RDWMLEKMT QLDTHVSAH DLKTVQALQ RRHDNLERE LAPVEEKVT KVMHLANSV KSAYPNERQ NVARRQDEI EGLWRQVKD KASERRARL ENAVGEQIF TNSCKDLLR WVADVKDQL NADNMVRDV QTAEALLKN HKDLGEEIK AKNDEFYEL TDLGKKLLK SNPELPEVA ERIERLTAE QAAIGRGWK EKQRWLDQC HQLQLFNRE ADNIDAATS AHQAFLEFS DLGNSLDEV EALQKQHKA FANTLFAQD DRVNLFSKK ADALIADDH YESKGIDDK RNEVLQRRQ AVKDLCQQR TNVLDASKN YQEFCAEVH DLRSWLNEK LKTASDESY RDLTNLERK LQKHEAFER ELRANEGQL RTVNKLGQA LIAQDSYRK DDVAKTLKE LNDEWQQLV GLSLEKGRR LRQAVVQHD YNTSIDDIQ TKLDEINEN LCSTNVGTD LRSCRDLLK RQDVLETEL GQCAARVDE LVNKSNDMM HDGHFDADA IRQKALDGQ KRLKQLEEP ARKRRDVLE EALKFYNFH FELDAELQW IKEHLPLAS SATLGQNLH QAQNLFKKH KKLEAEIVG HQPAIDKTL QAGQTLVDL KHPESAKIK DLCGVLQDA WDELNEKAS ERAQKLELS LKAQQFFFE ANEVESWLN EKADILAST DYGRDRDSA TKLLTKHKA LELELDTYN NIIVEMGRG AQNLIQNKH PDSKLIAER QSSLEHLVR SLQRKAVLR QHHLMESLF RHEYFLESE ELDRWIAEN LQQASSEDY GQDYEHLLI LQAKFDDLK HRIEAGAER FRQCEDLAQ KLIANESSY ISDIQKKQL QLETLTQSD AVEEVRQRH DVVRQSWQK LHDQMQNRE QRLLAAGEI HRFHRDVAD AMQRIQEKS AALGTDLGR DLNSALTLL RKHEAFENE LVALEAQLQ VLVEDGSKL QKIYPSNQS SIQLQQEVV IDAWDYLKE RAELRHDQL QASVDLQKF LTQVRNLTN WATALRLDM QAEENVRSI ARAQFLRNE HEGLKNEIE AREADFKDV AENLTAMEQ TGHYAANEA GERYKNLLQ ERERLHADW QIKKIHLDQ LCDLHMLLR EAKLIEDAT NAQEATLSN LDFGETVDE VANQVKKHE EFEKLIVHQ DEKFDTLVK TGQKLIGQQ HFDSKNIAK RLQEVGARR QQVHQLSDR KKQLLADAL LYAEFNRDV GEAQIWIAE KQKKLEKQV KTGEVTNLD DKIKELQKH QAFQAEIAA NEGKIKEVK SKGETLLAK KHKASKDIE VQLRNLDDA WRQLLREVD SRGKGLEEA QDILEFNNQ LDKIESWIR DKEVMIQAG DVGKDYEHC QALQRKLDD VDSDMRIDD TRIKTINSL ANKLAKQGH PGVQQRRDN FIKKWQDLQ GALAKYRNK LAAASEVHL FDRDVADTA ERINEKLLA METDDVGRS LPAVELLSR KQEALESEM TAVENKLQD HGKDAVLLS DKYPHAVQH LQGKMEELQ NEWEKLIAA RERRRNNLK GSHARQKFL SDVKDLEQW VSDTTKRME AHQPPSSVN EAETLLGLH DELKAEING RNEVFAKLI NFGRSFSES DDSDIIDGV NKLKELQSY IQQAWEQHK DALTYEYDL QDFKEQANQ LNNWLADKE AFLNNDDVG DTPRAVEAL IRKHEVFET MLTQPLSRI DELSRKKVG GKTPTDPTY ANSEVATKL NEIMARKNR LLGKAAERK KILHESKAL QKFLKNEYD VEVWLNHKL QIATDENYR EPYNLQNKI QKHVTFEAE VFANHERVT NVIEEGRDL IENKHYASK DIADRIEEL ENRWKELIE QSHLKRDRL NEAYQALLF NRSLDEFEA WLLEVEAQL TSTDVGKDL ATVNNLLKK HTILENDIQ QHTDNCETI NDAADQFVK NNHFMSEEI QQRAQDAIT RFHQLKEPV QQKRDLLEG SMMLQQFTR DVEDELQWL ADREPLAAS RDLGASLTA VQSLHKKHQ ALEAELSSR EPIIGSLVG RANSLARSG HVSAPLISQ KAKELQQKF ASIRDLASI RRLRLQDAL EVQTYYEEA TEAEAWMRD KRPSLVTRE VGKDEDSAE SLKRKLEAT ELEIKAFEV TIKKLKQSA DELIERQHY DAVNIEKRK NQLDEQFNE LRKLVSERE VRLDEALRY FTFVRECLD VQEWMKDQI LKTDSEEYG NDVEHVELL IQAFDTFHA SLMNSEPRI QSCIQNGNI LIEAKSSHS PEVQQKVAD IRDQWDDLL ELANARKDA LAGAKRVHV FDRTAEEII SWIQEKKAD LSYDTFGQD LESIQDLLR KHQALENEM KVIREKVEN VEQEGEKLI NEFPDTKEH IDDKCEDML SAWGSLQTE AARRKDHLQ QAEQLQAYF DQFQDLLAW ITEMVAKIT APDLPNDCN EAELLIERH KEYKVEISA KSNAFKQFY DSGNKFIEE GHLFASEIQ DKIKVLRQR LEFLNNTWE KRNLLYNQN LDVLLFKRE ADILDNWLA VREGTLKEG ITGDSIHHV EDLIRKHGD FEEAVKAQE DKFEALKRK TLIEDAFLL QREQEALAK KAEKERQEQ ERLEHLKRL EIQKINEKR RQERPQPPE IVEELVNGT KHEEEGRPV ATVRKTNSV VQTFERDRG GLRRGSDSA IQRSASMKV GTNVTGSKP KRTPTFTTR RRPSFKSKP TEAPPADAQ SFLDRKQLT TANGKRATN RTWKNSYTV LCGQLLCFF KNRDDFAAS KASGPPVNV HNAMCSIAD DYQKKKHTF RLELTDGSE FLFACSSDS EMDNWIQKI SFRARLPPS QQLLHLEIP KDQNDYEMS SQSSRTSSP DVADQVVLR HDPQSTNGS LSSLASSRH TMDGSGMSS KQDWQPNVQ RPQTMQPND APKSRLFER IFKKKRNTS QM
- >TC001109 RA
atgactcaa agggatgaa gtacagaag ttcgagcag ggccgaata cgcgtcctg caggaagaa cgtctgtac atacagaag aagacgttc accaaatgg atgaactcc tttcttcaa aaggttcgg atggaggtc gacgattta tttgtagat ttagcagac ggcaagaaa ttattaaaa cttttagaa ataatatcc ggtgaaaaa ctagcaaaa ccaaacaat ggcaaaatg cgggtacac aaaattgaa aacgtcaac aaaagtttg gcgtttttg cacacaaaa gttcggtta gaaagcata ggagccgaa gatattgtc gatgggaac ccccgactt atcttaggt ttaatctgg accatcatt ttaagattc caaattcaa gaaatcgaa attgacgtg gacgaggaa aacgaaagt tctgaaaag aaatcagcc aaagatgcc cttcttcta tgggcacag cgtaaaaca caggggtat aagggtgtt gatattcga gatttttca acatcgtgg cgcactggt ctaggcttt aatgcccta atccatgcc cacagaccg gacctgttt acttacgac gatttagta cataaccga aacattgat aacttaaac catgctttt gacgttgct aataacgaa ttgggaatt ccccggctt ttggatgcc gaagatatt gataattct agaccagac gagaaaagt atcatgact tacgtcgcc tcatattat cacactttt gcccgaatg aaaaatgaa gaaaagtcc ggacgacga attgccaaa ataattaaa caaatggtt gacgccgac aaaatgaaa gtcaattat gacgaacta accactgat ttactaaat tggataaaa attaaaatc gtagaactg gaagatcgc aattttccg aattcacta gaaggcatc caaacgctt tttctagcc tttgctcgc tacagaacg aaggaaaaa cctccgaaa tacatcgaa cggagcgaa atcgaagcc ttatttttc aacatcaac acccagctt aaagaactg agacaaccg gcttttatc ccacctgat ggtaaactc gtgcaagac atagaacgg gcttgggaa acccttgaa agggccgaa cacaaccgt gaagtggcc ctccgcaac gaattgctc cgacaagag cgtctcgaa caactttac tgcaaattc gaacgtaaa agtgtgctc cgagaaggt tacgttaaa gaaatgatc caagtctta tccgaccct cgatatggc tctaaccga gaccaagtt gatgctact gtcaagaaa cacgaagcc ataagtgct gatatttta gcgaggcaa gaacgtgtc aatgatttg actcaaatg tgcaatgaa ttggtgaga gaaaaatac agaaattcc gatcgggtt gtaaagaga gaggctgaa attttaaac caatggaaa cagttgtta gacctgttg gaaaaacat aaactgaaa ttgaatcgt atggtcaat atcaaatct ttggagatg gaaattgat acgactttg aacagcatg cagcagttg aaagccgat ttatcaact actgatacg ggaatacat ctgatggct gttgaggaa ttactgcac aaacatgcc ttacaagag ctacaagtg agctctttg ggcgaaact gaacggaga ctcaatcgt ctcgctgag agtacgttg gctcaaaat ccgcaagag gaaaaattg aaaaagaaa attcaagag ttgtctgcg acttacaaa gacttgcag gatgctagt gacaagaga aagggtctg cttgaagaa gcaagaaat ttctatcaa ttcctccaa gaccaagag gacgaagaa gcctggctg attgaaaaa cagcgaatt tgtcaagcc ggtattaca gctaaagac ttacgtggc gtattatct ctacagcaa aaacacaaa gtcctcatg gatgaaatc aaagcacgt agaaataaa ttcgatcaa ttaggagcg actggaaga caactaatc gcagaaaaa catcccagg tctaatgaa attcaacaa cacatggat cgcaacaaa caagcttgg agcgattta gaaaaacta gtcaatgat agaacaaaa cagttacaa gacgctgct gaaacttat cagttttac gccgatgct aacgaggct gattcgtgg ttgaatgaa aaaacgtcg attttgaat tctagtgat tacggaagt gacgaacca agcgcccaa gcacttctc caaagacat aaggacttg gaaggagag ttgaacgct tacaaaggg gatatttta agtttgaat acgcaagct gaccgtttg attgcgtca ggcatttcc aatctcgat ttaactgcc gaagttgaa gtagctgag ccggttgaa gaaatagtg tacgaaaat aggatgatt ccgactgaa gtttgggtt gaagaaccc gtggaaaaa gtcgagtac aaaatggtc gtcgaagag aaaaaggtg cctcaagtc aaagcattg tacccattt aatgaccat ggccttaac atggttaaa ggcgaagtg atgtatttg ttaaataag agcaacccg gattggtgg tgtgtacgt aaagcggac ggtactgac ggtttcgcc cccgctaaa tacgtaacg gagatcgaa cccaggttg ttacaaatg caataccgt aaacctgaa actgttaaa gttatgcag aaagttaaa aagacgaaa atggtgaat cagaaagtc ccagtcaag gttttcagg caacgcaaa cctgtgaaa aggaaagtt gacgacaat gatagtgtc ccgaaaaga cagaagaaa atcaacgat acgtatcaa cacctcctg gatgttgca gcaaaaaga catgctctc ttggaagac gccgtgaag ctgttccga ttttataaa gaatgtgac gattttgag aaatggatt aaagataaa gaaaaattg ctcgctgtt gacgatccc aatgacagt atcgagcaa gcaaaacgc aaatacgag aaatttgtg actgatttg tccgccaac aacaaacgc atggacgaa ctaaacgac gatgttaaa gaattcgag aaacagaaa cattcgcaa attgacaaa atcaaatcg cgccaccgc caagtccaa ggcgcttgg cagcgtctc aacttgctc aaagcccga aaagagaaa agtctcgaa ggagcaagt agtgttgag cttttccag aaaacttgt gacgaagct agggactgg atgttggag aaaatgact cagcttgat acccacgtt tcggctcac gacttaaaa accgttcaa gctttacaa cgtagacat gataatctc gaacgtgag ctcgcccct gttgaggaa aaagtgacc aaagtaatg catttggca aattcggtc aaatcagcg tatcctaat gagcgacaa aacgttgcg cgaagacaa gacgaaatc gaaggtttg tggcgacag gtcaaagac aaagctagt gaacgtaga gccaggctt gagaatgcc gttggtgag caaatattt accaattcg tgtaaagat cttttgcgg tgggtcgcc gatgttaaa gatcagttg aatgctgat aacatggtt agagacgtg cagactgct gaagcatta ctcaaaaat cataaggac ttgggggag gaaattaaa gctaagaat gacgaattc tacgagttg accgatttg ggtaaaaag cttctaaaa tctaatccc gaacttccc gaagttgcg gaacgtatc gaaagactg actgcggaa caagcggcc ataggtcgc ggttggaag gaaaaacag cgttggtta gaccagtgc caccaatta caactattc aacagagaa gccgataat atcgacgct gcaacttcg gcccatcaa gcatttttg gaattttcc gatttaggg aattccctt gatgaagtg gaagccttg caaaagcag cacaaagct ttcgctaac actttattc gcacaagac gaccgcgtc aatctcttc agcaaaaaa gccgacgcg ttaatcgct gatgaccat tatgaaagc aaaggaatc gacgataaa agaaacgaa gtcctgcaa cggcgccaa gctgttaaa gatttgtgc caacagaga acaaacgtt ctggacgcg tcgaaaaat tatcaagag ttttgtgcc gaagtgcac gatttgcgt agctggttg aatgagaaa cttaaaact gcatctgat gaaagttac cgagaccta actaacctg gagcgaaag ttacaaaaa cacgaagct tttgaacga gaattgaga gctaacgaa ggacagctt cgcacagtg aacaaatta ggccaagct ttgattgct caagacagt tacaggaag gacgacgtg gcgaaaact ttgaaagaa ctgaacgat gagtggcaa caactcgtc ggactttcc cttgaaaag gggagaagg ctgagacaa gccgttgtc caacacgac tataacact tcaattgac gatattcag accaaattg gatgaaatc aacgagaat ttgtgtagc actaacgtt ggaaccgat ttgagatct tgtcgcgat ttattaaaa cgccaagac gtgctcgag actgaactt ggacaatgt gcggccaga gtcgacgaa ttagtgaat aaaagtaac gatatgatg catgatggg cactttgat gccgacgca atccgccaa aaagccctc gacggccaa aaacgcctc aaacaacta gaagaaccg gccagaaaa cgacgagat gtcctagaa gaggctttg aaattctac aatttccat tttgaactc gacgctgag ttgcaatgg atcaaagag cacctccct ctcgcatcg tcggccact ctgggccaa aatctacac caagcccag aatctcttc aaaaaacac aaaaaactc gaagctgaa attgttggt catcaaccc gccattgat aaaaccttg caagctgga caaacccta gttgatttg aaacatcca gaaagtgcc aaaatcaaa gacttgtgt ggagtgctc caagacgcg tgggacgaa ttaaatgaa aaagcgagc gaacgtgca caaaaactg gaattgtcg ttgaaagct caacaattc ttctttgaa gcgaacgaa gttgagtcg tggctgaac gaaaaagct gatattttg gccagtact gactatggt agagatagg gattcagct acgaaattg ctgaccaaa cacaaggca ctcgaatta gaattggac acttacaac aacattata gtcgagatg ggacgtggt gcccaaaat ttgatacaa aataagcac ccagattcg aaattaatt gccgaaaga cagtccagt ttggaacat ttagttcgc tctttgcaa aggaaagct gttttaaga caacaccac cttatggag tcactgttt cgtcatgaa tactttctg gagtcggag gaattggat cggtggatt gccgaaaac ttacaacaa gcgtcttcg gaggattat ggacaagat tacgagcac cttctgatc cttcaagcc aaatttgac gatttgaaa caccgaatt gaagcagga gccgagcga ttccgccag tgcgaagac ttggcccag aagttaatc gccaatgaa agctcgtat atttcagat atacagaaa aagcaactt caactcgaa accctaaca caatcagac gcagtcgag gaagttagg cagaggcat gacgttgta cggcaatcg tggcaaaaa ctgcacgac cagatgcag aaccgcgag cagcggctt cttgcagcc ggtgaaatc caccgtttc catcgcgat gtggccgat gccatgcaa cgaatccaa gaaaagagc gctgcttta ggcaccgac ctaggacgc gacctaaac tccgcctta acactactt agaaaacac gaagctttc gaaaacgaa ttggtggca cttgaggct caattacaa gtcctggtt gaagatgga tcaaaatta caaaaaatc tacccaagt aatcaatct agcattcaa cttcaacaa gaagtagtg atcgatgct tgggattac ttgaaagaa cgagcagaa ttgagacac gaccagctc caggctagt gtggatttg caaaaattc ctaacccaa gtcagaaat ttgacaaat tgggcgact gcgttaaga ctagacatg caagctgag gaaaacgtg aggagtatc gcgagagca cagtttcta cgtaacgaa cacgaaggt ttgaagaat gagattgaa gcgagggaa gccgatttt aaagacgtt gccgaaaat ttaacagcg atggaacaa accgggcac tatgcagca aatgaagcc ggcgaaaga tacaaaaac ttgcttcag gagcgagaa agacttcac gccgactgg cagataaag aaaatacac ttggaccaa ttgtgcgac ttgcatatg ctactaaga gaggcaaaa ctgatcgaa gacgccacc aatgcacaa gaagccact ttgagtaac ctagatttt ggcgaaact gtcgacgaa gtcgccaat caagtcaag aaacacgaa gaatttgaa aaactaatc gtccatcag gacgaaaag ttcgacact cttgttaag accggccaa aaattaatc ggccaacag catttcgac agcaaaaac atcgctaaa cgtttgcaa gaagtcgga gccaggcgg caacaagtc caccaattg agcgatcgc aagaaacaa ctcctcgcc gatgctctg ttgtatgcg gaattcaat agagacgtg ggcgaggcc cagatatgg atagccgag aaacaaaag aagttggaa aagcaagta aagaccggc gaagtgacc aatctggac gacaaaatc aaagaacta cagaaacac caagcgttc caagccgaa atcgcagcc aatgagggc aaaatcaag gaagttaag tctaaggga gagactctt ttagcaaag aaacacaaa gcttcgaaa gacattgaa gtgcaattg aggaatttg gacgatgct tggaggcaa cttttgcgc gaggtggac tcgcgtggt aaaggtctg gaagaggcg caggatatt ttagaattc aacaatcaa ctggacaag atcgagtca tggatcagg gacaaagaa gtgatgatt caagccggc gacgtgggg aaagactac gaacattgc caagcgttg cagagaaaa ctcgacgat gtggatagc gatatgcgg atcgacgat acccggatc aaaaccatc aattccctg gctaacaaa ctggcaaag caagggcac cccggagtg caacaaaga cgcgacaat ttcatcaag aaatggcag gatttgcaa ggcgctttg gccaaatat cgcaataaa ttagctgca gcttccgaa gttcacttg tttgatcgc gatgtcgct gatacggct gagagaatt aatgaaaaa ctgctcgcg atggaaact gacgacgtc ggtcgcagt ttacccgcc gttgagctg ctttcgcga aaacaggaa gcactagag agtgaaatg accgccgtt gaaaataaa ttgcaggat catggaaaa gatgctgtt ttattgagt gataaatac ccgcacgct gtccaacac ctccagggt aaaatggaa gagttgcag aacgaatgg gagaagctg atcgcagcg agagaacgt cgcagaaat aatcttaaa ggatcgcat gccaggcaa aaattctta tcagatgtt aaggatttg gaacagtgg gttagtgac acgactaaa cgaatggaa gcacaccaa ccgccaagt agtgtcaac gaggctgag acactttta ggcttacat gatgaactt aaagccgaa atcaatgga cgtaacgag gtttttgcg aaactgatc aatttcggg cgcagtttt tccgaaagc gacgattct gatataatt gacggagtg aacaaattg aaggagctt cagagttac attcagcaa gcttgggag caacacaaa gacgcacta acgtacgaa tacgacctc caagatttc aaagaacaa gccaaccag ttaaacaat tggttagcc gataaggaa gctttcttg aataacgac gacgtgggg gatacacct cgcgcagtt gaagctctc atcagaaaa cacgaagtt tttgaaaca atgttaacg caaccactg tcacgtatc gatgaattg tcgcgtaaa aaagtagga ggtaaaaca ccaactgac ccaacttac gcaaacagc gaagtcgca acaaaatta aacgagata atggctcgc aaaaaccga cttttgggc aaagcagca gagcgcaag aaaatccta cacgaatca aaagcatta caaaaattc ctcaaaaac gaatacgac gttgaggtt tggttgaac cacaagctt caaatcgca accgacgaa aattatcgc gaaccttac aacttgcaa aacaaaatc caaaaacat gtcactttc gaagccgaa gtttttgca aaccatgaa cgcgtcacc aacgtaatt gaagaaggg cgagacttg atcgaaaat aaacactac gctagcaaa gacattgcc gaccgaatt gaggaattg gaaaatcgg tggaaggaa ttaattgaa cagtcacac ctgaaacgt gaccgactg aacgaagct tatcaagcg ttacttttc aaccgatct ctcgatgaa ttcgaagct tggttgctc gaagtcgaa gcccaacta acatccaca gatgtcggc aaagatttg gcaactgtt aacaaccta ctcaagaaa cacacaatt cttgaaaac gacattcag caacacact gataattgt gaaacgatc aacgatgcg gctgatcag ttcgttaaa aataatcat ttcatgagt gaagagatt caacagagg gctcaggac gctattact cgattccat caactcaaa gagcccgtt caacagaag agagatttg ttagagggg tcgatgatg ttgcaacaa ttcactcga gatgttgaa gacgaatta caatggttg gccgatagg gagccactt gccgcctcc agagacttg ggagcaagt ttgacagcc gtacaaagc ttacacaag aaacaccaa gcgcttgaa gctgaatta tcatcaaga gaaccaatt attggatcg cttgtaggt cgtgcaaat tctctcgcg agatcgggg catgtttca gccccgctt atcagtcag aaagcgaag gaattgcaa cagaaattc gcaagtatt agggatcta gccagtatc aggagattg agactacaa gatgctttg gaagttcaa acgtattat gaagaggca acagaagct gaggcatgg atgcgggac aaacgcccg agtttagtg accagagaa gtcgggaaa gatgaagac tcagccgaa tctttaaaa cgtaaattg gaagccacc gaattagaa ataaaagcg ttcgaagtg actattaaa aaattgaaa cagtcggcc gatgagcta atcgagaga caacattac gatgctgtt aacatcgaa aagagaaag aatcaactc gatgagcaa tttaacgaa ctgcgaaaa ctggtttcg gagcgtgaa gttcggctc gacgaagcc cttcgttac tttactttt gtgcgcgaa tgtttggac gtccaggaa tggatgaaa gaccaaata cttaaaacc gattccgaa gaatacgga aatgatgtc gaacacgtc gaactacta atccaagct tttgacaca ttccatgca agtctaatg aacagcgaa cctcgaatt caatcctgt atccagaac ggaaatatc ctaattgaa gctaaaagc agccacagt ccggaagtg caacaaaaa gtggccgat atccgcgac caatgggac gatttgctc gaacttgcc aacgcgcgt aaagacgcc ttagctggg gccaaacga gtgcatgtt ttcgatcgc acagctgag gaaattatt tcgtggatc caggaaaag aaagccgac ttatcgtac gatacattc gggcaagac ttggaaagt atccaagat cttctcaga aaacaccaa gcgttggag aatgagatg aaagtgatt agggaaaaa gtggaaaat gtggaacaa gaaggcgag aaacttata aacgagttt ccggatacc aaagaacat attgacgat aagtgcgag gatatgttg tcagcttgg gggagtctt caaaccgaa gcagcgcgg agaaaagat cacttacag caagccgaa caacttcaa gcttacttc gaccagttc caagattta ttggcgtgg attacggaa atggtggct aaaatcaca gcacctgac cttccaaat gactgcaac gaggccgag cttttgatc gaacggcac aaagagtac aaagttgaa atcagtgca aaatcaaac gcttttaaa caattctac gactccggt aataaattc atcgaagag ggtcacctg ttcgctagc gaaatccaa gacaaaatc aaagtgtta cgccagaga ctggagttt ttgaataac acgtgggag aaacgcaat ttgctctac aaccaaaat ttggacgtc ctcttgttc aaacgcgag gcagatatt ttggataat tggctagct gtgagggaa ggtacccta aaggaggga attactgga gatagcatc caccacgtg gaagatttg atccgaaaa cacggcgat ttcgaagaa gcagtcaaa gcacaagag gacaagttt gaagcactc aaacgcaaa actttaatt gaagatgcg ttccttctc caacgggaa caggaagct ttggccaaa aaagccgag aaagagcgc caggaacag gaacgtttg gagcacttg aaacgactc gaaatccag aaaatcaac gaaaaaaga aggcaagaa agaccccaa ccgccggaa attgtcgag gaactggtc aatgggacc aaacacgag gaagaaggc cgtccggtt gcaacagtt cgtaaaaca aattccgtt gttcagacg tttgagaga gaccgtggt gggttaagg agagggtca gatagtgca attcaacgg tcggcaagc atgaaagtt ggtacaaat gttacggga agtaagccg aaaagaact cctactttt actacgagg cgaaggcca tcgttcaag tctaaacca accgaggca ccacctgcg gacgcccag tcgttcctc gatcgcaaa caattaacc acagcaaat ggaaaacgc gccactaac aggacttgg aagaattca tacacggtt ttgtgcgga cagttattg tgtttcttt aaaaatcgc gatgatttt gcggctagt aaagctagt ggtccaccg gttaatgtt cataatgcg atgtgttca atagcggat gattaccaa aagaaaaaa catacgttt agacttgag cttacagac ggttcggaa tttttgttt gcgtgtagt tcggattcg gagatggat aattggatt cagaaaatt agtttcagg gctaggttg cctccaagt cagcaattg ctgcatttg gaaattcca aaggatcaa aacgattac gagatgagt tcacaaagt agcaggacc tctagtccg gacgttgcc gatcaagtt gtcttgcgg cacgatccg caatcaacc aatggcagt ttatcaagt ttagcatct tcgcgtcat actatggat ggtagcgga atgtcgtca aaacaagat tggcaacct aatgtacaa cgtccacaa acgatgcaa cctaatgat gcaccaaag tcacgtctg tttgaaaga attttcaaa aagaaacgg aacacttcg caaatgtaa
- >TC001109 RA
cattttcaa tataagcaa aaatgtttt tgttacagt tggcttcgt gcattatga acctagagg gcaaacctt tgtgcaaca tcgaaccaa catgactca aagggatga agtacagaa gttcgagca gggccgaat acgcgtcct gcaggaaga acgtctgta catacagaa gaagacgtt caccaaatg gatgaactc ctttcttca aaaggttcg gatggaggt cgacgattt atttgtaga tttagcaga cggcaagaa attattaaa acttttaga aataatatc cggtgaaaa actagcaaa accaaacaa tggcaaaat gcgggtaca caaaattga aaacgtcaa caaaagttt ggcgttttt gcacacaaa agttcggtt agaaagcat aggagccga agatattgt cgatgggaa cccccgact tatcttagg tttaatctg gaccatcat tttaagatt ccaaattca agaaatcga aattgacgt ggacgagga aaacgaaag ttctgaaaa gaaatcagc caaagatgc ccttcttct atgggcaca gcgtaaaac acaggggta taagggtgt tgatattcg agatttttc aacatcgtg gcgcactgg tctaggctt taatgccct aatccatgc ccacagacc ggacctgtt tacttacga cgatttagt acataaccg aaacattga taacttaaa ccatgcttt tgacgttgc taataacga attgggaat tccccggct tttggatgc cgaagatat tgataattc tagaccaga cgagaaaag tatcatgac ttacgtcgc ctcatatta tcacacttt tgcccgaat gaaaaatga agaaaagtc cggacgacg aattgccaa aataattaa acaaatggt tgacgccga caaaatgaa agtcaatta tgacgaact aaccactga tttactaaa ttggataaa aattaaaat cgtagaact ggaagatcg caattttcc gaattcact agaaggcat ccaaacgct ttttctagc ctttgctcg ctacagaac gaaggaaaa acctccgaa atacatcga acggagcga aatcgaagc cttattttt caacatcaa cacccagct taaagaact gagacaacc ggcttttat cccacctga tggtaaact cgtgcaaga catagaacg ggcttggga aacccttga aagggccga acacaaccg tgaagtggc cctccgcaa cgaattgct ccgacaaga gcgtctcga acaacttta ctgcaaatt cgaacgtaa aagtgtgct ccgagaagg ttacgttaa agaaatgat ccaagtctt atccgaccc tcgatatgg ctctaaccg agaccaagt tgatgctac tgtcaagaa acacgaagc cataagtgc tgatatttt agcgaggca agaacgtgt caatgattt gactcaaat gtgcaatga attggtgag agaaaaata cagaaattc cgatcgggt tgtaaagag agaggctga aattttaaa ccaatggaa acagttgtt agacctgtt ggaaaaaca taaactgaa attgaatcg tatggtcaa tatcaaatc tttggagat ggaaattga tacgacttt gaacagcat gcagcagtt gaaagccga tttatcaac tactgatac gggaataca tctgatggc tgttgagga attactgca caaacatgc cttacaaga gctacaagt gagctcttt gggcgaaac tgaacggag actcaatcg tctcgctga gagtacgtt ggctcaaaa tccgcaaga ggaaaaatt gaaaaagaa aattcaaga gttgtctgc gacttacaa agacttgca ggatgctag tgacaagag aaagggtct gcttgaaga agcaagaaa tttctatca attcctcca agaccaaga ggacgaaga agcctggct gattgaaaa acagcgaat ttgtcaagc cggtattac agctaaaga cttacgtgg cgtattatc tctacagca aaaacacaa agtcctcat ggatgaaat caaagcacg tagaaataa attcgatca attaggagc gactggaag acaactaat cgcagaaaa acatcccag gtctaatga aattcaaca acacatgga tcgcaacaa acaagcttg gagcgattt agaaaaact agtcaatga tagaacaaa acagttaca agacgctgc tgaaactta tcagtttta cgccgatgc taacgaggc tgattcgtg gttgaatga aaaaacgtc gattttgaa ttctagtga ttacggaag tgacgaacc aagcgccca agcacttct ccaaagaca taaggactt ggaaggaga gttgaacgc ttacaaagg ggatatttt aagtttgaa tacgcaagc tgaccgttt gattgcgtc aggcatttc caatctcga tttaactgc cgaagttga agtagctga gccggttga agaaatagt gtacgaaaa taggatgat tccgactga agtttgggt tgaagaacc cgtggaaaa agtcgagta caaaatggt cgtcgaaga gaaaaaggt gcctcaagt caaagcatt gtacccatt taatgacca tggccttaa catggttaa aggcgaagt gatgtattt gttaaataa gagcaaccc ggattggtg gtgtgtacg taaagcgga cggtactga cggtttcgc ccccgctaa atacgtaac ggagatcga acccaggtt gttacaaat gcaataccg taaacctga aactgttaa agttatgca gaaagttaa aaagacgaa aatggtgaa tcagaaagt cccagtcaa ggttttcag gcaacgcaa acctgtgaa aaggaaagt tgacgacaa tgatagtgt cccgaaaag acagaagaa aatcaacga tacgtatca acacctcct ggatgttgc agcaaaaag acatgctct cttggaaga cgccgtgaa gctgttccg attttataa agaatgtga cgattttga gaaatggat taaagataa agaaaaatt gctcgctgt tgacgatcc caatgacag tatcgagca agcaaaacg caaatacga gaaatttgt gactgattt gtccgccaa caacaaacg catggacga actaaacga cgatgttaa agaattcga gaaacagaa acattcgca aattgacaa aatcaaatc gcgccaccg ccaagtcca aggcgcttg gcagcgtct caacttgct caaagcccg aaaagagaa aagtctcga aggagcaag tagtgttga gcttttcca gaaaacttg tgacgaagc tagggactg gatgttgga gaaaatgac tcagcttga tacccacgt ttcggctca cgacttaaa aaccgttca agctttaca acgtagaca tgataatct cgaacgtga gctcgcccc tgttgagga aaaagtgac caaagtaat gcatttggc aaattcggt caaatcagc gtatcctaa tgagcgaca aaacgttgc gcgaagaca agacgaaat cgaaggttt gtggcgaca ggtcaaaga caaagctag tgaacgtag agccaggct tgagaatgc cgttggtga gcaaatatt taccaattc gtgtaaaga tcttttgcg gtgggtcgc cgatgttaa agatcagtt gaatgctga taacatggt tagagacgt gcagactgc tgaagcatt actcaaaaa tcataagga cttggggga ggaaattaa agctaagaa tgacgaatt ctacgagtt gaccgattt gggtaaaaa gcttctaaa atctaatcc cgaacttcc cgaagttgc ggaacgtat cgaaagact gactgcgga acaagcggc cataggtcg cggttggaa ggaaaaaca gcgttggtt agaccagtg ccaccaatt acaactatt caacagaga agccgataa tatcgacgc tgcaacttc ggcccatca agcattttt ggaattttc cgatttagg gaattccct tgatgaagt ggaagcctt gcaaaagca gcacaaagc tttcgctaa cactttatt cgcacaaga cgaccgcgt caatctctt cagcaaaaa agccgacgc gttaatcgc tgatgacca ttatgaaag caaaggaat cgacgataa aagaaacga agtcctgca acggcgcca agctgttaa agatttgtg ccaacagag aacaaacgt tctggacgc gtcgaaaaa ttatcaaga gttttgtgc cgaagtgca cgatttgcg tagctggtt gaatgagaa acttaaaac tgcatctga tgaaagtta ccgagacct aactaacct ggagcgaaa gttacaaaa acacgaagc ttttgaacg agaattgag agctaacga aggacagct tcgcacagt gaacaaatt aggccaagc tttgattgc tcaagacag ttacaggaa ggacgacgt ggcgaaaac tttgaaaga actgaacga tgagtggca acaactcgt cggactttc ccttgaaaa ggggagaag gctgagaca agccgttgt ccaacacga ctataacac ttcaattga cgatattca gaccaaatt ggatgaaat caacgagaa tttgtgtag cactaacgt tggaaccga tttgagatc ttgtcgcga tttattaaa acgccaaga cgtgctcga gactgaact tggacaatg tgcggccag agtcgacga attagtgaa taaaagtaa cgatatgat gcatgatgg gcactttga tgccgacgc aatccgcca aaaagccct cgacggcca aaaacgcct caaacaact agaagaacc ggccagaaa acgacgaga tgtcctaga agaggcttt gaaattcta caatttcca ttttgaact cgacgctga gttgcaatg gatcaaaga gcacctccc tctcgcatc gtcggccac tctgggcca aaatctaca ccaagccca gaatctctt caaaaaaca caaaaaact cgaagctga aattgttgg tcatcaacc cgccattga taaaacctt gcaagctgg acaaaccct agttgattt gaaacatcc agaaagtgc caaaatcaa agacttgtg tggagtgct ccaagacgc gtgggacga attaaatga aaaagcgag cgaacgtgc acaaaaact ggaattgtc gttgaaagc tcaacaatt cttctttga agcgaacga agttgagtc gtggctgaa cgaaaaagc tgatatttt ggccagtac tgactatgg tagagatag ggattcagc tacgaaatt gctgaccaa acacaaggc actcgaatt agaattgga cacttacaa caacattat agtcgagat gggacgtgg tgcccaaaa tttgataca aaataagca cccagattc gaaattaat tgccgaaag acagtccag tttggaaca tttagttcg ctctttgca aaggaaagc tgttttaag acaacacca ccttatgga gtcactgtt tcgtcatga atactttct ggagtcgga ggaattgga tcggtggat tgccgaaaa cttacaaca agcgtcttc ggaggatta tggacaaga ttacgagca ccttctgat ccttcaagc caaatttga cgatttgaa acaccgaat tgaagcagg agccgagcg attccgcca gtgcgaaga cttggccca gaagttaat cgccaatga aagctcgta tatttcaga tatacagaa aaagcaact tcaactcga aaccctaac acaatcaga cgcagtcga ggaagttag gcagaggca tgacgttgt acggcaatc gtggcaaaa actgcacga ccagatgca gaaccgcga gcagcggct tcttgcagc cggtgaaat ccaccgttt ccatcgcga tgtggccga tgccatgca acgaatcca agaaaagag cgctgcttt aggcaccga cctaggacg cgacctaaa ctccgcctt aacactact tagaaaaca cgaagcttt cgaaaacga attggtggc acttgaggc tcaattaca agtcctggt tgaagatgg atcaaaatt acaaaaaat ctacccaag taatcaatc tagcattca acttcaaca agaagtagt gatcgatgc ttgggatta cttgaaaga acgagcaga attgagaca cgaccagct ccaggctag tgtggattt gcaaaaatt cctaaccca agtcagaaa tttgacaaa ttgggcgac tgcgttaag actagacat gcaagctga ggaaaacgt gaggagtat cgcgagagc acagtttct acgtaacga acacgaagg tttgaagaa tgagattga agcgaggga agccgattt taaagacgt tgccgaaaa tttaacagc gatggaaca aaccgggca ctatgcagc aaatgaagc cggcgaaag atacaaaaa cttgcttca ggagcgaga aagacttca cgccgactg gcagataaa gaaaataca cttggacca attgtgcga cttgcatat gctactaag agaggcaaa actgatcga agacgccac caatgcaca agaagccac tttgagtaa cctagattt tggcgaaac tgtcgacga agtcgccaa tcaagtcaa gaaacacga agaatttga aaaactaat cgtccatca ggacgaaaa gttcgacac tcttgttaa gaccggcca aaaattaat cggccaaca gcatttcga cagcaaaaa catcgctaa acgtttgca agaagtcgg agccaggcg gcaacaagt ccaccaatt gagcgatcg caagaaaca actcctcgc cgatgctct gttgtatgc ggaattcaa tagagacgt gggcgaggc ccagatatg gatagccga gaaacaaaa gaagttgga aaagcaagt aaagaccgg cgaagtgac caatctgga cgacaaaat caaagaact acagaaaca ccaagcgtt ccaagccga aatcgcagc caatgaggg caaaatcaa ggaagttaa gtctaaggg agagactct tttagcaaa gaaacacaa agcttcgaa agacattga agtgcaatt gaggaattt ggacgatgc ttggaggca acttttgcg cgaggtgga ctcgcgtgg taaaggtct ggaagaggc gcaggatat tttagaatt caacaatca actggacaa gatcgagtc atggatcag ggacaaaga agtgatgat tcaagccgg cgacgtggg gaaagacta cgaacattg ccaagcgtt gcagagaaa actcgacga tgtggatag cgatatgcg gatcgacga tacccggat caaaaccat caattccct ggctaacaa actggcaaa gcaagggca ccccggagt gcaacaaag acgcgacaa tttcatcaa gaaatggca ggatttgca aggcgcttt ggccaaata tcgcaataa attagctgc agcttccga agttcactt gtttgatcg cgatgtcgc tgatacggc tgagagaat taatgaaaa actgctcgc gatggaaac tgacgacgt cggtcgcag tttacccgc cgttgagct gctttcgcg aaaacagga agcactaga gagtgaaat gaccgccgt tgaaaataa attgcagga tcatggaaa agatgctgt tttattgag tgataaata cccgcacgc tgtccaaca cctccaggg taaaatgga agagttgca gaacgaatg ggagaagct gatcgcagc gagagaacg tcgcagaaa taatcttaa aggatcgca tgccaggca aaaattctt atcagatgt taaggattt ggaacagtg ggttagtga cacgactaa acgaatgga agcacacca accgccaag tagtgtcaa cgaggctga gacactttt aggcttaca tgatgaact taaagccga aatcaatgg acgtaacga ggtttttgc gaaactgat caatttcgg gcgcagttt ttccgaaag cgacgattc tgatataat tgacggagt gaacaaatt gaaggagct tcagagtta cattcagca agcttggga gcaacacaa agacgcact aacgtacga atacgacct ccaagattt caaagaaca agccaacca gttaaacaa ttggttagc cgataagga agctttctt gaataacga cgacgtggg ggatacacc tcgcgcagt tgaagctct catcagaaa acacgaagt ttttgaaac aatgttaac gcaaccact gtcacgtat cgatgaatt gtcgcgtaa aaaagtagg aggtaaaac accaactga cccaactta cgcaaacag cgaagtcgc aacaaaatt aaacgagat aatggctcg caaaaaccg acttttggg caaagcagc agagcgcaa gaaaatcct acacgaatc aaaagcatt acaaaaatt cctcaaaaa cgaatacga cgttgaggt ttggttgaa ccacaagct tcaaatcgc aaccgacga aaattatcg cgaacctta caacttgca aaacaaaat ccaaaaaca tgtcacttt cgaagccga agtttttgc aaaccatga acgcgtcac caacgtaat tgaagaagg gcgagactt gatcgaaaa taaacacta cgctagcaa agacattgc cgaccgaat tgaggaatt ggaaaatcg gtggaagga attaattga acagtcaca cctgaaacg tgaccgact gaacgaagc ttatcaagc gttactttt caaccgatc tctcgatga attcgaagc ttggttgct cgaagtcga agcccaact aacatccac agatgtcgg caaagattt ggcaactgt taacaacct actcaagaa acacacaat tcttgaaaa cgacattca gcaacacac tgataattg tgaaacgat caacgatgc ggctgatca gttcgttaa aaataatca tttcatgag tgaagagat tcaacagag ggctcagga cgctattac tcgattcca tcaactcaa agagcccgt tcaacagaa gagagattt gttagaggg gtcgatgat gttgcaaca attcactcg agatgttga agacgaatt acaatggtt ggccgatag ggagccact tgccgcctc cagagactt gggagcaag tttgacagc cgtacaaag cttacacaa gaaacacca agcgcttga agctgaatt atcatcaag agaaccaat tattggatc gcttgtagg tcgtgcaaa ttctctcgc gagatcggg gcatgtttc agccccgct tatcagtca gaaagcgaa ggaattgca acagaaatt cgcaagtat tagggatct agccagtat caggagatt gagactaca agatgcttt ggaagttca aacgtatta tgaagaggc aacagaagc tgaggcatg gatgcggga caaacgccc gagtttagt gaccagaga agtcgggaa agatgaaga ctcagccga atctttaaa acgtaaatt ggaagccac cgaattaga aataaaagc gttcgaagt gactattaa aaaattgaa acagtcggc cgatgagct aatcgagag acaacatta cgatgctgt taacatcga aaagagaaa gaatcaact cgatgagca atttaacga actgcgaaa actggtttc ggagcgtga agttcggct cgacgaagc ccttcgtta ctttacttt tgtgcgcga atgtttgga cgtccagga atggatgaa agaccaaat acttaaaac cgattccga agaatacgg aaatgatgt cgaacacgt cgaactact aatccaagc ttttgacac attccatgc aagtctaat gaacagcga acctcgaat tcaatcctg tatccagaa cggaaatat cctaattga agctaaaag cagccacag tccggaagt gcaacaaaa agtggccga tatccgcga ccaatggga cgatttgct cgaacttgc caacgcgcg taaagacgc cttagctgg ggccaaacg agtgcatgt tttcgatcg cacagctga ggaaattat ttcgtggat ccaggaaaa gaaagccga cttatcgta cgatacatt cgggcaaga cttggaaag tatccaaga tcttctcag aaaacacca agcgttgga gaatgagat gaaagtgat tagggaaaa agtggaaaa tgtggaaca agaaggcga gaaacttat aaacgagtt tccggatac caaagaaca tattgacga taagtgcga ggatatgtt gtcagcttg ggggagtct tcaaaccga agcagcgcg gagaaaaga tcacttaca gcaagccga acaacttca agcttactt cgaccagtt ccaagattt attggcgtg gattacgga aatggtggc taaaatcac agcacctga ccttccaaa tgactgcaa cgaggccga gcttttgat cgaacggca caaagagta caaagttga aatcagtgc aaaatcaaa cgcttttaa acaattcta cgactccgg taataaatt catcgaaga gggtcacct gttcgctag cgaaatcca agacaaaat caaagtgtt acgccagag actggagtt tttgaataa cacgtggga gaaacgcaa tttgctcta caaccaaaa tttggacgt cctcttgtt caaacgcga ggcagatat tttggataa ttggctagc tgtgaggga aggtaccct aaaggaggg aattactgg agatagcat ccaccacgt ggaagattt gatccgaaa acacggcga tttcgaaga agcagtcaa agcacaaga ggacaagtt tgaagcact caaacgcaa aactttaat tgaagatgc gttccttct ccaacggga acaggaagc tttggccaa aaaagccga gaaagagcg ccaggaaca ggaacgttt ggagcactt gaaacgact cgaaatcca gaaaatcaa cgaaaaaag aaggcaaga aagacccca accgccgga aattgtcga ggaactggt caatgggac caaacacga ggaagaagg ccgtccggt tgcaacagt tcgtaaaac aaattccgt tgttcagac gtttgagag agaccgtgg tgggttaag gagagggtc agatagtgc aattcaacg gtcggcaag catgaaagt tggtacaaa tgttacggg aagtaagcc gaaaagaac tcctacttt tactacgag gcgaaggcc atcgttcaa gtctaaacc aaccgaggc accacctgc ggacgccca gtcgttcct cgatcgcaa acaattaac cacagcaaa tggaaaacg cgccactaa caggacttg gaagaattc atacacggt tttgtgcgg acagttatt gtgtttctt taaaaatcg cgatgattt tgcggctag taaagctag tggtccacc ggttaatgt tcataatgc gatgtgttc aatagcgga tgattacca aaagaaaaa acatacgtt tagacttga gcttacaga cggttcgga atttttgtt tgcgtgtag ttcggattc ggagatgga taattggat tcagaaaat tagtttcag ggctaggtt gcctccaag tcagcaatt gctgcattt ggaaattcc aaaggatca aaacgatta cgagatgag ttcacaaag tagcaggac ctctagtcc ggacgttgc cgatcaagt tgtcttgcg gcacgatcc gcaatcaac caatggcag tttatcaag tttagcatc ttcgcgtca tactatgga tggtagcgg aatgtcgtc aaaacaaga ttggcaacc taatgtaca acgtccaca aacgatgca acctaatga tgcaccaaa gtcacgtct gtttgaaag aattttcaa aaagaaacg gaacacttc gcaaatgta atccgcatt acatacagt aatagttag gagcctttt agtattatg tcaatatat ttttgttta cattcttgt acatagttt cgtagttaa atatatgaa cattatata aatccttca tagattttt attatgaca aaatacatg tttaaatta tcc
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