| General information | |||
| TC001106 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11006242..11029743 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001106 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03137 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001106 RA
MPLLRTTTL IALTVIQLF EPSSLTVNQ DKRISAFLN GDFIIGALF PVHHQPSSK KRTVASLGI KCGEIRELY GIQRVEVTF QTLDKINKD PNLLPNLTL GVEIRDECW YAPVALQQS IELIRDAIT PVSSYLDSC HNLTTSDAG QRGPLIGVV GPGSSSVAL QVQNLLQLF HIPQVGYST TSRDLSDKS RFNYFLRVV PSDYYQAQV ILDIVRYYG WTYVSAVNT DENYGQSGI QAFRELAEE ADVCIARED SILSNAPDE GFDKVILNL KQDPNAYVV VCFCEGLTV RGLLKATRR LNMTNHFLF IGSDGWADR GDVTRGFEE EAWGALSIR IHSPYVKSF DEYYWSLKP HNNTRNPWF REFWESRFS CSLDGPPDD DSASVDTLN STQSSTVNK TDSRKLCSG EESLLDKYR QDSKLSFVI KAVYSLAHA LHDMQQDKC GPGTTGTCE KLLPFNGSL FKSYLMNVT FEYDEDIIE FDENGDPPG RYDIMNYQK LDDGSFDYV QVGNWHNRS LWWNQSRPM QFGRSRNVK SVCSEECPK GHYKNVQQG GKDKRCCWV CVPCPSGEI LDEQGESCH RCPQGFVPD ELKLKCLLL PIEHMQWWD TQAIVAMIF ASLGFLSTF FAFMVFMKY NNTPVVKSS TKELCYIIL AGMTVSHAS VFAILTKPS MISCALSRV LPGLSFAMM YAALLTKTN RIARILAGS KKRFPTRKP LFMSATAQV VITCFLIAI EVFISGGML HYQFPNIAH VYSTRRTLL ECDTTPEAI VVPLAFDFV LIILCTIYA VKTRNVPEN FNEAKFIGF AMYTTCVIW IAFVPIYFG SDSKVITLC MCVTLSAMV IWVFLFVPK LYIILVKPE RNNRALFTT SKTIRCHIG SRVASAVSE KSSNNSWKE STVSGRDVP PQKRTLSCQ TGNELLQVL LNPKALMEP YTVSANSFA LPKITERDC CEADNCQVK KIIIELPDN PVLETISRG VNRDL
- >TC001106 RA
atgccgctc cttcggacc acaaccctt atcgccttg acggtgatc cagctattt gagccctca tccctaaca gtcaaccaa gacaaacgc atatcagcg ttcctgaac ggcgatttc ataatcggt gccttattc cccgtgcac caccagccg tcatcaaag aaacgcacc gtcgccagc ctgggcatc aaatgcggg gaaattcgc gaactgtac ggcatccag cgcgtcgaa gtcactttc cagacatta gacaagatc aacaaagac cccaatttg ctgcccaac ctcactcta ggggtggag atccgcgac gagtgctgg tatgccccg gtggcgctc cagcagagc atcgagctc atcagggat gccatcacc cccgtgtcc tcctacctg gactcctgc cacaacctc acgacctcc gacgccggc cagcggggg ccccttatc ggggttgtg gggccgggg tccagctcg gtggcgctg caagtccag aacttgctc cagttgttc catattcca caagtgggg tattccacc acttcgagg gatttgtcc gataagagc agatttaat tatttcttg agggtggtg ccgtcggat tattaccaa gcgcaagtt attctcgat attgtgaga tattacggt tggacgtac gtctcggct gtcaatacc gatgaaaat tatggccag tcggggatc caagcgttt cgggagctg gccgaagaa gccgacgtt tgcattgca cgggaggat tcgattctg agcaatgcg cccgacgaa ggcttcgac aaagttatc ctcaatctg aagcaggat cccaacgct tacgtcgtc gtctgcttt tgcgaagga ctgaccgtc agaggactc ctcaaggcg actcggcgt ctcaacatg accaatcat ttcttattc ataggaagt gatggttgg gctgaccga ggcgatgta actcgcggt tttgaagag gaggcctgg ggtgcactt tcgattcga attcactcc ccgtacgtg aagtccttc gacgagtac tactggtcc ttgaagccc cataacaac acccgtaat ccatggttt agggaattc tgggagtcc cggttttcg tgctcgctt gacggaccg ccagacgat gactctgcg tccgtcgat actctcaac tccacacaa agctctacc gtcaacaag acagactcg agaaagctt tgcagcggt gaagagagt ttgctagac aaatatcgg caagattct aagctcagc tttgtcatt aaagctgtg tacagttta gcgcacgcc ttgcatgac atgcagcag gacaagtgt gggcccgga acgaccgga acgtgtgaa aagctcctg cccttcaat gggtccttg tttaagagt tatttgatg aacgttacc tttgagtac gacgaagat ataatagag tttgatgaa aacggggat cccccgggg cgttatgac attatgaat tatcagaag ctcgacgat gggagcttt gattatgtc caggtcggt aactggcat aacagaagt ttgtggtgg aatcagtcg agaccgatg cagttcggt agaagcaga aatgtcaag tcggtttgc tcagaggaa tgcccgaaa ggacattac aaaaacgtc cagcagggc ggaaaggac aagcgctgt tgctgggtg tgcgtgcca tgtcccagc ggagagatc ctagatgag caaggagag agttgccac cgctgcccg caagggttc gtgccggac gagctgaaa cttaagtgt ctcctgctg cccatcgag cacatgcaa tggtgggac acgcaggcc atcgtcgcc atgatcttc gcctctctg ggcttcctc agcacgttc ttcgccttc atggtcttc atgaagtac aacaacacg ccggtcgtc aagtcctcc accaaggag ctgtgctac atcatcctg gcgggcatg accgtctcg cacgctagc gtgttcgca attctgacg aagccgtcg atgatctcg tgcgcgctg agccgggtc ttgcccggc ctgagcttc gccatgatg tatgcggcg ctgttgacc aaaaccaac cgaatcgcc cggatcctg gcggggagc aagaagcgc tttcccacg aggaaaccc ttgttcatg tcggcgacg gcccaagtc gtcatcacg tgcttcctg atcgctatc gaggttttc atctcgggg gggatgctc cattaccaa ttccccaac atcgcacat gtgtactcc accaggagg acgctcctg gagtgcgac acgaccccg gaagccatc gttgtcccg ctcgccttt gacttcgtc ctcatcatc ctctgcacc atctacgcc gtcaagacg cggaacgtc cccgagaac ttcaacgaa gccaaattc atcggcttc gccatgtac accacctgc gtcatctgg atcgccttc gtccccatc tacttcggg agcgactcg aaagtcatc acgctgtgc atgtgcgtg acgcttagt gcgatggtg atttgggtc tttcttttc gtcccgaaa ctctatata attctcgtg aagccggaa cgaaataac cgggcactt ttcacgacc agtaagact atcaggtgc cacattggc tcaagagtg gcgtctgca gtttcggag aaaagctca aataacagt tggaaggag agcacggtt tcagggagg gatgtaccc ccacaaaaa agaacgctg tcgtgtcag acgggaaac gaacttttg caagtttta ctgaaccct aaagctctc atggaacca tacacagta tcagccaac tcttttgcc cttcccaaa attacagag agggactgt tgcgaggct gataactgc caagtcaaa aaaatcatc atcgagctg cctgacaat ccggttttg gaaaccatt agtcggggg gtaaaccga gacctttga
- >TC001106 RA
cttacgaaa agctcgaac ctcgtggtt tcatgaatt cgatttcaa tccaccgaa aatgactgc gcgtttcct cccctcttc caagcggtg ccaaagcca cgatgccgc tccttcgga ccacaaccc ttatcgcct tgacggtga tccagctat ttgagccct catccctaa cagtcaacc aagacaaac gcatatcag cgttcctga acggcgatt tcataatcg gtgccttat tccccgtgc accaccagc cgtcatcaa agaaacgca ccgtcgcca gcctgggca tcaaatgcg gggaaattc gcgaactgt acggcatcc agcgcgtcg aagtcactt tccagacat tagacaaga tcaacaaag accccaatt tgctgccca acctcactc taggggtgg agatccgcg acgagtgct ggtatgccc cggtggcgc tccagcaga gcatcgagc tcatcaggg atgccatca cccccgtgt cctcctacc tggactcct gccacaacc tcacgacct ccgacgccg gccagcggg ggcccctta tcggggttg tggggccgg ggtccagct cggtggcgc tgcaagtcc agaacttgc tccagttgt tccatattc cacaagtgg ggtattcca ccacttcga gggatttgt ccgataaga gcagattta attatttct tgagggtgg tgccgtcgg attattacc aagcgcaag ttattctcg atattgtga gatattacg gttggacgt acgtctcgg ctgtcaata ccgatgaaa attatggcc agtcgggga tccaagcgt ttcgggagc tggccgaag aagccgacg tttgcattg cacgggagg attcgattc tgagcaatg cgcccgacg aaggcttcg acaaagtta tcctcaatc tgaagcagg atcccaacg cttacgtcg tcgtctgct tttgcgaag gactgaccg tcagaggac tcctcaagg cgactcggc gtctcaaca tgaccaatc atttcttat tcataggaa gtgatggtt gggctgacc gaggcgatg taactcgcg gttttgaag aggaggcct ggggtgcac tttcgattc gaattcact ccccgtacg tgaagtcct tcgacgagt actactggt ccttgaagc cccataaca acacccgta atccatggt ttagggaat tctgggagt cccggtttt cgtgctcgc ttgacggac cgccagacg atgactctg cgtccgtcg atactctca actccacac aaagctcta ccgtcaaca agacagact cgagaaagc tttgcagcg gtgaagaga gtttgctag acaaatatc ggcaagatt ctaagctca gctttgtca ttaaagctg tgtacagtt tagcgcacg ccttgcatg acatgcagc aggacaagt gtgggcccg gaacgaccg gaacgtgtg aaaagctcc tgcccttca atgggtcct tgtttaaga gttatttga tgaacgtta cctttgagt acgacgaag atataatag agtttgatg aaaacgggg atcccccgg ggcgttatg acattatga attatcaga agctcgacg atgggagct ttgattatg tccaggtcg gtaactggc ataacagaa gtttgtggt ggaatcagt cgagaccga tgcagttcg gtagaagca gaaatgtca agtcggttt gctcagagg aatgcccga aaggacatt acaaaaacg tccagcagg gcggaaagg acaagcgct gttgctggg tgtgcgtgc catgtccca gcggagaga tcctagatg agcaaggag agagttgcc accgctgcc cgcaagggt tcgtgccgg acgagctga aacttaagt gtctcctgc tgcccatcg agcacatgc aatggtggg acacgcagg ccatcgtcg ccatgatct tcgcctctc tgggcttcc tcagcacgt tcttcgcct tcatggtct tcatgaagt acaacaaca cgccggtcg tcaagtcct ccaccaagg agctgtgct acatcatcc tggcgggca tgaccgtct cgcacgcta gcgtgttcg caattctga cgaagccgt cgatgatct cgtgcgcgc tgagccggg tcttgcccg gcctgagct tcgccatga tgtatgcgg cgctgttga ccaaaacca accgaatcg cccggatcc tggcgggga gcaagaagc gctttccca cgaggaaac ccttgttca tgtcggcga cggcccaag tcgtcatca cgtgcttcc tgatcgcta tcgaggttt tcatctcgg gggggatgc tccattacc aattcccca acatcgcac atgtgtact ccaccagga ggacgctcc tggagtgcg acacgaccc cggaagcca tcgttgtcc cgctcgcct ttgacttcg tcctcatca tcctctgca ccatctacg ccgtcaaga cgcggaacg tccccgaga acttcaacg aagccaaat tcatcggct tcgccatgt acaccacct gcgtcatct ggatcgcct tcgtcccca tctacttcg ggagcgact cgaaagtca tcacgctgt gcatgtgcg tgacgctta gtgcgatgg tgatttggg tctttcttt tcgtcccga aactctata taattctcg tgaagccgg aacgaaata accgggcac ttttcacga ccagtaaga ctatcaggt gccacattg gctcaagag tggcgtctg cagtttcgg agaaaagct caaataaca gttggaagg agagcacgg tttcaggga gggatgtac ccccacaaa aaagaacgc tgtcgtgtc agacgggaa acgaacttt tgcaagttt tactgaacc ctaaagctc tcatggaac catacacag tatcagcca actcttttg cccttccca aaattacag agagggact gttgcgagg ctgataact gccaagtca aaaaaatca tcatcgagc tgcctgaca atccggttt tggaaacca ttagtcggg gggtaaacc gagaccttt gaacaaaat ttacgaaat tttttttga gtctgctaa tcaaaaacg ggtttactc ctcgaccaa aatttaatt aagaaacgc tgtaaaatt ctgaataaa tgtgaaact agtca
