Status
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Gene information
General information
TC001106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11006242..11029743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001106
    iBeetle screen iB_03137

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001106 RA
      MPLLRTTTL IALTVIQLF EPSSLTVNQ DKRISAFLN 
      GDFIIGALF PVHHQPSSK KRTVASLGI KCGEIRELY 
      GIQRVEVTF QTLDKINKD PNLLPNLTL GVEIRDECW 
      YAPVALQQS IELIRDAIT PVSSYLDSC HNLTTSDAG 
      QRGPLIGVV GPGSSSVAL QVQNLLQLF HIPQVGYST 
      TSRDLSDKS RFNYFLRVV PSDYYQAQV ILDIVRYYG 
      WTYVSAVNT DENYGQSGI QAFRELAEE ADVCIARED 
      SILSNAPDE GFDKVILNL KQDPNAYVV VCFCEGLTV 
      RGLLKATRR LNMTNHFLF IGSDGWADR GDVTRGFEE 
      EAWGALSIR IHSPYVKSF DEYYWSLKP HNNTRNPWF 
      REFWESRFS CSLDGPPDD DSASVDTLN STQSSTVNK 
      TDSRKLCSG EESLLDKYR QDSKLSFVI KAVYSLAHA 
      LHDMQQDKC GPGTTGTCE KLLPFNGSL FKSYLMNVT 
      FEYDEDIIE FDENGDPPG RYDIMNYQK LDDGSFDYV 
      QVGNWHNRS LWWNQSRPM QFGRSRNVK SVCSEECPK 
      GHYKNVQQG GKDKRCCWV CVPCPSGEI LDEQGESCH 
      RCPQGFVPD ELKLKCLLL PIEHMQWWD TQAIVAMIF 
      ASLGFLSTF FAFMVFMKY NNTPVVKSS TKELCYIIL 
      AGMTVSHAS VFAILTKPS MISCALSRV LPGLSFAMM 
      YAALLTKTN RIARILAGS KKRFPTRKP LFMSATAQV 
      VITCFLIAI EVFISGGML HYQFPNIAH VYSTRRTLL 
      ECDTTPEAI VVPLAFDFV LIILCTIYA VKTRNVPEN 
      FNEAKFIGF AMYTTCVIW IAFVPIYFG SDSKVITLC 
      MCVTLSAMV IWVFLFVPK LYIILVKPE RNNRALFTT 
      SKTIRCHIG SRVASAVSE KSSNNSWKE STVSGRDVP 
      PQKRTLSCQ TGNELLQVL LNPKALMEP YTVSANSFA 
      LPKITERDC CEADNCQVK KIIIELPDN PVLETISRG 
      VNRDL
    CDS sequence
    • >TC001106 RA
      atgccgctc cttcggacc acaaccctt atcgccttg 
      acggtgatc cagctattt gagccctca tccctaaca 
      gtcaaccaa gacaaacgc atatcagcg ttcctgaac 
      ggcgatttc ataatcggt gccttattc cccgtgcac 
      caccagccg tcatcaaag aaacgcacc gtcgccagc 
      ctgggcatc aaatgcggg gaaattcgc gaactgtac 
      ggcatccag cgcgtcgaa gtcactttc cagacatta 
      gacaagatc aacaaagac cccaatttg ctgcccaac 
      ctcactcta ggggtggag atccgcgac gagtgctgg 
      tatgccccg gtggcgctc cagcagagc atcgagctc 
      atcagggat gccatcacc cccgtgtcc tcctacctg 
      gactcctgc cacaacctc acgacctcc gacgccggc 
      cagcggggg ccccttatc ggggttgtg gggccgggg 
      tccagctcg gtggcgctg caagtccag aacttgctc 
      cagttgttc catattcca caagtgggg tattccacc 
      acttcgagg gatttgtcc gataagagc agatttaat 
      tatttcttg agggtggtg ccgtcggat tattaccaa 
      gcgcaagtt attctcgat attgtgaga tattacggt 
      tggacgtac gtctcggct gtcaatacc gatgaaaat 
      tatggccag tcggggatc caagcgttt cgggagctg 
      gccgaagaa gccgacgtt tgcattgca cgggaggat 
      tcgattctg agcaatgcg cccgacgaa ggcttcgac 
      aaagttatc ctcaatctg aagcaggat cccaacgct 
      tacgtcgtc gtctgcttt tgcgaagga ctgaccgtc 
      agaggactc ctcaaggcg actcggcgt ctcaacatg 
      accaatcat ttcttattc ataggaagt gatggttgg 
      gctgaccga ggcgatgta actcgcggt tttgaagag 
      gaggcctgg ggtgcactt tcgattcga attcactcc 
      ccgtacgtg aagtccttc gacgagtac tactggtcc 
      ttgaagccc cataacaac acccgtaat ccatggttt 
      agggaattc tgggagtcc cggttttcg tgctcgctt 
      gacggaccg ccagacgat gactctgcg tccgtcgat 
      actctcaac tccacacaa agctctacc gtcaacaag 
      acagactcg agaaagctt tgcagcggt gaagagagt 
      ttgctagac aaatatcgg caagattct aagctcagc 
      tttgtcatt aaagctgtg tacagttta gcgcacgcc 
      ttgcatgac atgcagcag gacaagtgt gggcccgga 
      acgaccgga acgtgtgaa aagctcctg cccttcaat 
      gggtccttg tttaagagt tatttgatg aacgttacc 
      tttgagtac gacgaagat ataatagag tttgatgaa 
      aacggggat cccccgggg cgttatgac attatgaat 
      tatcagaag ctcgacgat gggagcttt gattatgtc 
      caggtcggt aactggcat aacagaagt ttgtggtgg 
      aatcagtcg agaccgatg cagttcggt agaagcaga 
      aatgtcaag tcggtttgc tcagaggaa tgcccgaaa 
      ggacattac aaaaacgtc cagcagggc ggaaaggac 
      aagcgctgt tgctgggtg tgcgtgcca tgtcccagc 
      ggagagatc ctagatgag caaggagag agttgccac 
      cgctgcccg caagggttc gtgccggac gagctgaaa 
      cttaagtgt ctcctgctg cccatcgag cacatgcaa 
      tggtgggac acgcaggcc atcgtcgcc atgatcttc 
      gcctctctg ggcttcctc agcacgttc ttcgccttc 
      atggtcttc atgaagtac aacaacacg ccggtcgtc 
      aagtcctcc accaaggag ctgtgctac atcatcctg 
      gcgggcatg accgtctcg cacgctagc gtgttcgca 
      attctgacg aagccgtcg atgatctcg tgcgcgctg 
      agccgggtc ttgcccggc ctgagcttc gccatgatg 
      tatgcggcg ctgttgacc aaaaccaac cgaatcgcc 
      cggatcctg gcggggagc aagaagcgc tttcccacg 
      aggaaaccc ttgttcatg tcggcgacg gcccaagtc 
      gtcatcacg tgcttcctg atcgctatc gaggttttc 
      atctcgggg gggatgctc cattaccaa ttccccaac 
      atcgcacat gtgtactcc accaggagg acgctcctg 
      gagtgcgac acgaccccg gaagccatc gttgtcccg 
      ctcgccttt gacttcgtc ctcatcatc ctctgcacc 
      atctacgcc gtcaagacg cggaacgtc cccgagaac 
      ttcaacgaa gccaaattc atcggcttc gccatgtac 
      accacctgc gtcatctgg atcgccttc gtccccatc 
      tacttcggg agcgactcg aaagtcatc acgctgtgc 
      atgtgcgtg acgcttagt gcgatggtg atttgggtc 
      tttcttttc gtcccgaaa ctctatata attctcgtg 
      aagccggaa cgaaataac cgggcactt ttcacgacc 
      agtaagact atcaggtgc cacattggc tcaagagtg 
      gcgtctgca gtttcggag aaaagctca aataacagt 
      tggaaggag agcacggtt tcagggagg gatgtaccc 
      ccacaaaaa agaacgctg tcgtgtcag acgggaaac 
      gaacttttg caagtttta ctgaaccct aaagctctc 
      atggaacca tacacagta tcagccaac tcttttgcc 
      cttcccaaa attacagag agggactgt tgcgaggct 
      gataactgc caagtcaaa aaaatcatc atcgagctg 
      cctgacaat ccggttttg gaaaccatt agtcggggg 
      gtaaaccga gacctttga
    mRNA sequence
    • >TC001106 RA
      cttacgaaa agctcgaac ctcgtggtt tcatgaatt 
      cgatttcaa tccaccgaa aatgactgc gcgtttcct 
      cccctcttc caagcggtg ccaaagcca cgatgccgc 
      tccttcgga ccacaaccc ttatcgcct tgacggtga 
      tccagctat ttgagccct catccctaa cagtcaacc 
      aagacaaac gcatatcag cgttcctga acggcgatt 
      tcataatcg gtgccttat tccccgtgc accaccagc 
      cgtcatcaa agaaacgca ccgtcgcca gcctgggca 
      tcaaatgcg gggaaattc gcgaactgt acggcatcc 
      agcgcgtcg aagtcactt tccagacat tagacaaga 
      tcaacaaag accccaatt tgctgccca acctcactc 
      taggggtgg agatccgcg acgagtgct ggtatgccc 
      cggtggcgc tccagcaga gcatcgagc tcatcaggg 
      atgccatca cccccgtgt cctcctacc tggactcct 
      gccacaacc tcacgacct ccgacgccg gccagcggg 
      ggcccctta tcggggttg tggggccgg ggtccagct 
      cggtggcgc tgcaagtcc agaacttgc tccagttgt 
      tccatattc cacaagtgg ggtattcca ccacttcga 
      gggatttgt ccgataaga gcagattta attatttct 
      tgagggtgg tgccgtcgg attattacc aagcgcaag 
      ttattctcg atattgtga gatattacg gttggacgt 
      acgtctcgg ctgtcaata ccgatgaaa attatggcc 
      agtcgggga tccaagcgt ttcgggagc tggccgaag 
      aagccgacg tttgcattg cacgggagg attcgattc 
      tgagcaatg cgcccgacg aaggcttcg acaaagtta 
      tcctcaatc tgaagcagg atcccaacg cttacgtcg 
      tcgtctgct tttgcgaag gactgaccg tcagaggac 
      tcctcaagg cgactcggc gtctcaaca tgaccaatc 
      atttcttat tcataggaa gtgatggtt gggctgacc 
      gaggcgatg taactcgcg gttttgaag aggaggcct 
      ggggtgcac tttcgattc gaattcact ccccgtacg 
      tgaagtcct tcgacgagt actactggt ccttgaagc 
      cccataaca acacccgta atccatggt ttagggaat 
      tctgggagt cccggtttt cgtgctcgc ttgacggac 
      cgccagacg atgactctg cgtccgtcg atactctca 
      actccacac aaagctcta ccgtcaaca agacagact 
      cgagaaagc tttgcagcg gtgaagaga gtttgctag 
      acaaatatc ggcaagatt ctaagctca gctttgtca 
      ttaaagctg tgtacagtt tagcgcacg ccttgcatg 
      acatgcagc aggacaagt gtgggcccg gaacgaccg 
      gaacgtgtg aaaagctcc tgcccttca atgggtcct 
      tgtttaaga gttatttga tgaacgtta cctttgagt 
      acgacgaag atataatag agtttgatg aaaacgggg 
      atcccccgg ggcgttatg acattatga attatcaga 
      agctcgacg atgggagct ttgattatg tccaggtcg 
      gtaactggc ataacagaa gtttgtggt ggaatcagt 
      cgagaccga tgcagttcg gtagaagca gaaatgtca 
      agtcggttt gctcagagg aatgcccga aaggacatt 
      acaaaaacg tccagcagg gcggaaagg acaagcgct 
      gttgctggg tgtgcgtgc catgtccca gcggagaga 
      tcctagatg agcaaggag agagttgcc accgctgcc 
      cgcaagggt tcgtgccgg acgagctga aacttaagt 
      gtctcctgc tgcccatcg agcacatgc aatggtggg 
      acacgcagg ccatcgtcg ccatgatct tcgcctctc 
      tgggcttcc tcagcacgt tcttcgcct tcatggtct 
      tcatgaagt acaacaaca cgccggtcg tcaagtcct 
      ccaccaagg agctgtgct acatcatcc tggcgggca 
      tgaccgtct cgcacgcta gcgtgttcg caattctga 
      cgaagccgt cgatgatct cgtgcgcgc tgagccggg 
      tcttgcccg gcctgagct tcgccatga tgtatgcgg 
      cgctgttga ccaaaacca accgaatcg cccggatcc 
      tggcgggga gcaagaagc gctttccca cgaggaaac 
      ccttgttca tgtcggcga cggcccaag tcgtcatca 
      cgtgcttcc tgatcgcta tcgaggttt tcatctcgg 
      gggggatgc tccattacc aattcccca acatcgcac 
      atgtgtact ccaccagga ggacgctcc tggagtgcg 
      acacgaccc cggaagcca tcgttgtcc cgctcgcct 
      ttgacttcg tcctcatca tcctctgca ccatctacg 
      ccgtcaaga cgcggaacg tccccgaga acttcaacg 
      aagccaaat tcatcggct tcgccatgt acaccacct 
      gcgtcatct ggatcgcct tcgtcccca tctacttcg 
      ggagcgact cgaaagtca tcacgctgt gcatgtgcg 
      tgacgctta gtgcgatgg tgatttggg tctttcttt 
      tcgtcccga aactctata taattctcg tgaagccgg 
      aacgaaata accgggcac ttttcacga ccagtaaga 
      ctatcaggt gccacattg gctcaagag tggcgtctg 
      cagtttcgg agaaaagct caaataaca gttggaagg 
      agagcacgg tttcaggga gggatgtac ccccacaaa 
      aaagaacgc tgtcgtgtc agacgggaa acgaacttt 
      tgcaagttt tactgaacc ctaaagctc tcatggaac 
      catacacag tatcagcca actcttttg cccttccca 
      aaattacag agagggact gttgcgagg ctgataact 
      gccaagtca aaaaaatca tcatcgagc tgcctgaca 
      atccggttt tggaaacca ttagtcggg gggtaaacc 
      gagaccttt gaacaaaat ttacgaaat tttttttga 
      gtctgctaa tcaaaaacg ggtttactc ctcgaccaa 
      aatttaatt aagaaacgc tgtaaaatt ctgaataaa 
      tgtgaaact agtca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY