Status
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Gene information
General information
TC001105 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11002802..11004796
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001105
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03136

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    egg chamber degenerated - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001105 RA
      MPQSPKKPA PKWHHPSEV MKLHKLKMK KKALQARIA 
      GGTLNTSLS DAQDNKNAA LEDIISAKK RKNPFVKTS 
      ESKKCKQDV PSSLDESTD QTLFKLLHQ STPSTTCNL 
      TSFENILKK EIEPEVVVV KAQGQNDIP IDWTLKTKI 
      RLWSEQQFS WSQKLKISE EASGTTGFV RCLDQNTDT 
      NLNISPNSK FHQCCLYWQ QPSLPWLTL FPRASGKVS 
      ESDSSIVTS VAMKTALQE AWKDSLKSL FQLIRTKQC 
      PYFYVCSNN FTVLFRAAG ISGMGDIHA LVTPTTRGF 
      RQMLRQEDI EFKMPLKKK RTSDQGYET FDSATGESP 
      EDEESPDEQ WLQSMGINA DDIKQINYT QAKIVHKAE 
      CEVDNSEQS LVLIEGVEV HALYNFLIN CKSATSLTG 
      PLAGIPPTL LAPVAFNGA TLRALKVRE SKVHVDNSY 
      YHSLEISGP ILPSTIHNL FSINQSDHS MSATFVNVA 
      STLPFSKVL SNEDQDAKR NFIFDKENL SDCGLSRKI 
      LGQFCKADV CVTNVECLK YSGDNKTYT WT
    CDS sequence
    • >TC001105 RA
      atgcctcaa agccccaaa aagccggcc ccaaaatgg 
      caccacccg tccgaagtc atgaaattg cacaaattg 
      aaaatgaag aagaaggcc cttcaggcg cgcattgcg 
      gggggcact ctcaacaca tcgctctcc gacgcccag 
      gacaacaaa aacgcagct ttagaagac atcatttcg 
      gccaaaaaa cgcaaaaac ccgtttgtg aaaaccagc 
      gaaagcaaa aaatgcaag caagatgtt ccctctagt 
      cttgacgag tcaactgat cagacgtta ttcaagctc 
      ttgcatcaa agtactcca agtacgacg tgcaatttg 
      acctcattc gagaatatt ttaaagaaa gaaatcgag 
      cctgaagtg gtggttgtg aaggcacag ggccagaac 
      gatattccc attgactgg actttaaaa accaagata 
      cgtttatgg tcagaacag caattttcc tggagccag 
      aaattaaaa atcagtgaa gaagcatcg gggacaaca 
      ggatttgtt cgttgtttg gaccaaaac actgacaca 
      aacttaaat atctcccca aactcaaag tttcaccag 
      tgttgtctc tactggcaa caaccgtct ctcccatgg 
      ctcactctc tttcctcga gcctctgga aaggtctca 
      gagtctgac tcgagcatt gttacgagc gttgcaatg 
      aaaaccgcc ttacaagag gcctggaaa gacagttta 
      aagtcccta ttccagtta attaggaca aaacagtgt 
      ccgtacttt tacgtctgt tctaataac tttactgtg 
      ttgtttcgg gcggcggga attagcgga atgggcgat 
      atacatgcg ttagttact cccacaact cggggattc 
      cgtcagatg ctcagacag gaagatatt gaatttaaa 
      atgccgcta aagaagaaa cggacctcg gatcaggga 
      tacgaaacg tttgattct gcaaccggg gaatccccc 
      gaagacgag gaatcgcca gacgagcag tggttgcaa 
      agtatggga attaacgcc gatgacatc aagcaaatt 
      aattacacg caagctaag attgtccac aaagctgaa 
      tgcgaggtg gacaacagt gagcagagt ctagtctta 
      atcgaggga gtggaagtg cacgccctt tacaatttc 
      ctgattaat tgtaaaagt gcgacaagt ctgaccggg 
      cctttggcg gggattccc cccacgctt ttggcccct 
      gtggcgttc aatggagcg acgttgagg gcactgaaa 
      gtgcgcgaa agcaaagtc cacgttgac aattcctat 
      tatcactcg ctcgaaatt tcgggacca attttaccg 
      agcacaatt cataattta ttttcaatt aatcaaagc 
      gaccacagt atgagcgct acgttcgtt aatgtggcg 
      tccacactg ccgtttagt aaagtttta agtaacgaa 
      gatcaagac gcgaagaga aattttatt tttgataag 
      gaaaatttg tcagactgt gggttaagc agaaaaatt 
      ctggggcag ttttgcaaa gcggacgtt tgtgttaca 
      aacgttgag tgtctgaag tatagtggc gacaataaa 
      acttacacc tggacgtaa
    mRNA sequence
    • >TC001105 RA
      gcggccata aaatatggt tatgttgtg acgcttcaa 
      tgtttcgtt gataaattg tgttgtctg aattaattc 
      ttcccattt gtggcttcg agacaatgc ctcaaagcc 
      ccaaaaagc cggccccaa aatggcacc acccgtccg 
      aagtcatga aattgcaca aattgaaaa tgaagaaga 
      aggcccttc aggcgcgca ttgcggggg gcactctca 
      acacatcgc tctccgacg cccaggaca acaaaaacg 
      cagctttag aagacatca tttcggcca aaaaacgca 
      aaaacccgt ttgtgaaaa ccagcgaaa gcaaaaaat 
      gcaagcaag atgttccct ctagtcttg acgagtcaa 
      ctgatcaga cgttattca agctcttgc atcaaagta 
      ctccaagta cgacgtgca atttgacct cattcgaga 
      atattttaa agaaagaaa tcgagcctg aagtggtgg 
      ttgtgaagg cacagggcc agaacgata ttcccattg 
      actggactt taaaaacca agatacgtt tatggtcag 
      aacagcaat tttcctgga gccagaaat taaaaatca 
      gtgaagaag catcgggga caacaggat ttgttcgtt 
      gtttggacc aaaacactg acacaaact taaatatct 
      ccccaaact caaagtttc accagtgtt gtctctact 
      ggcaacaac cgtctctcc catggctca ctctctttc 
      ctcgagcct ctggaaagg tctcagagt ctgactcga 
      gcattgtta cgagcgttg caatgaaaa ccgccttac 
      aagaggcct ggaaagaca gtttaaagt ccctattcc 
      agttaatta ggacaaaac agtgtccgt acttttacg 
      tctgttcta ataacttta ctgtgttgt ttcgggcgg 
      cgggaatta gcggaatgg gcgatatac atgcgttag 
      ttactccca caactcggg gattccgtc agatgctca 
      gacaggaag atattgaat ttaaaatgc cgctaaaga 
      agaaacgga cctcggatc agggatacg aaacgtttg 
      attctgcaa ccggggaat cccccgaag acgaggaat 
      cgccagacg agcagtggt tgcaaagta tgggaatta 
      acgccgatg acatcaagc aaattaatt acacgcaag 
      ctaagattg tccacaaag ctgaatgcg aggtggaca 
      acagtgagc agagtctag tcttaatcg agggagtgg 
      aagtgcacg ccctttaca atttcctga ttaattgta 
      aaagtgcga caagtctga ccgggcctt tggcgggga 
      ttcccccca cgcttttgg cccctgtgg cgttcaatg 
      gagcgacgt tgagggcac tgaaagtgc gcgaaagca 
      aagtccacg ttgacaatt cctattatc actcgctcg 
      aaatttcgg gaccaattt taccgagca caattcata 
      atttatttt caattaatc aaagcgacc acagtatga 
      gcgctacgt tcgttaatg tggcgtcca cactgccgt 
      ttagtaaag ttttaagta acgaagatc aagacgcga 
      agagaaatt ttatttttg ataaggaaa atttgtcag 
      actgtgggt taagcagaa aaattctgg ggcagtttt 
      gcaaagcgg acgtttgtg ttacaaacg ttgagtgtc 
      tgaagtata gtggcgaca ataaaactt acacctgga 
      cgtaattat taatttatt gtactattt ttatttatt 
      gaataaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY