Status
Please wait ...
Gene information
General information
TC001089 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10813599..10815614
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001089
    iBeetle screen iB_03135

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001089 RA
      MAEDGEEKP RNFHEMELD DRILKAIAK LGWQTPTLI 
      QERAIPLLL EGKDVLVRA RTGSGKTAA FTIPVIQKI 
      LTLKKTAKH QEIKALILA PSKELCHQI CGVIKELTV 
      KCSREIRCV DVAPQVELS VQKPLLVEQ PDIVVGTPT 
      RVLKHIKAG YMDLKTSME LLVIDEADL VFSFGYESE 
      VKELLERLP SIYQAILAS ATLSEDVKN LKSLVLHNP 
      VILKLEEPE IAPASQLSH YHLMAEEMD KATILYALL 
      KLHLIRGKT IIFVNTVDK CYKIKLYLE QFGIPTCVL 
      NSELPAKIR CHSVNQFNQ GIYDTIVAS DEKALEQPG 
      NPNDPELKK SKRKKDKES GVSRGIDFQ CVANVINFD 
      FPLDVQSYV HRAGRTARG NNQGSVLSF VSIREKPLL 
      EQVETHFKS DQDDVSIFK SYQFKLDEV ESFKYRAKD 
      AWRAVTRIA VREARLKEI KQEIFNCQK LKSYFEDNP 
      TDLQVLRHD KALHTVKIQ QHLSDVPEY IVPPTLKGI 
      ASLGKKKRK REYYRTSQG SSSRFQAKK NNPLLSMDF 
      EGFNKKKKK N
    CDS sequence
    • >TC001089 RA
      atggcagaa gacggtgag gaaaaacct cgcaatttt 
      cacgaaatg gagctggac gaccgcatt ttaaaggct 
      atagcaaaa ctgggatgg cagactcct acacttatc 
      caagaacgg gcgatccct cttctttta gagggcaaa 
      gacgttctt gtaagagcc cgtacaggt tctggtaaa 
      acggcagca tttaccatc cccgtaatt cagaaaatt 
      ttgactctt aaaaaaacc gcgaaacac caagaaatt 
      aaagcgctt attctcgcc ccgagtaaa gaattgtgc 
      caccagata tgtggcgtt attaaagag ttgacagtt 
      aagtgttca cgtgaaatt cggtgtgtc gatgtggcc 
      ccgcaggtt gagttaagt gtgcaaaag cccttgcta 
      gtggaacag cctgatatt gtcgtgggg actcccaca 
      agagtgcta aagcacatc aaggcgggg tacatggat 
      ctgaagaca agcatggaa ttgttggtg atagatgaa 
      gccgatttg gttttttct tttggttat gagagcgag 
      gtgaaggag ttgttggag cgtttgccg agcatatat 
      caggctatt ttggcctcg gccacgtta agtgaagac 
      gtgaaaaat ctcaaaagt ttagtgtta cacaatcct 
      gtaatttta aaacttgag gaacctgaa attgcgcct 
      gcaagccaa ctcagtcat taccactta atggccgag 
      gaaatggac aaagccacg attttatac gccctttta 
      aaactgcac ttaattagg gggaagacc atcattttt 
      gttaacacc gtcgataag tgttacaaa attaaattg 
      tacttggaa cagtttgga attccaacc tgtgttcta 
      aactccgag cttcctgcg aaaatacgg tgtcattcg 
      gtaaatcag ttcaatcag ggcatttat gacaccatc 
      gtggcctcg gacgaaaaa gcgttggaa caaccggga 
      aacccgaac gacccagag ttaaaaaaa tcaaaaagg 
      aagaaggac aaagaaagc ggtgtttct agaggaatc 
      gattttcag tgcgttgcc aatgttatt aattttgat 
      tttccactt gacgtgcaa tcctacgtg catagagcg 
      ggaagaaca gctagggga aacaaccaa ggcagtgtt 
      ttatcgttt gtgagcata cgagaaaaa cctttgcta 
      gaacaggtt gaaactcac ttcaaaagc gaccaagat 
      gatgtttca attttcaag tcgtaccag ttcaaatta 
      gacgaagtt gagtctttt aaatatcga gcaaaagac 
      gcatggcgt gctgtaacg agaattgca gtacgtgaa 
      gcaagactg aaggaaatc aaacaagag atttttaat 
      tgtcaaaaa ttgaaaagt tactttgag gataatcca 
      actgatctt caagtttta aggcacgat aaggcgctg 
      catacggtt aaaattcag cagcatttg tctgatgtt 
      cccgaatat attgttcca ccgactttg aagggtatt 
      gccagctta gggaagaag aaaaggaaa cgggaatat 
      tataggaca tcgcaaggg tcaagcagc aggttccag 
      gcgaagaaa aacaatcct ctgttaagc atggacttt 
      gaaggtttt aacaaaaag aaaaagaag aactag
    mRNA sequence
    • >TC001089 RA
      aatttttca aaaataatg gcagaagac ggtgaggaa 
      aaacctcgc aattttcac gaaatggag ctggacgac 
      cgcatttta aaggctata gcaaaactg ggatggcag 
      actcctaca cttatccaa gaacgggcg atccctctt 
      cttttagag ggcaaagac gttcttgta agagcccgt 
      acaggttct ggtaaaacg gcagcattt accatcccc 
      gtaattcag aaaattttg actcttaaa aaaaccgcg 
      aaacaccaa gaaattaaa gcgcttatt ctcgccccg 
      agtaaagaa ttgtgccac cagatatgt ggcgttatt 
      aaagagttg acagttaag tgttcacgt gaaattcgg 
      tgtgtcgat gtggccccg caggttgag ttaagtgtg 
      caaaagccc ttgctagtg gaacagcct gatattgtc 
      gtggggact cccacaaga gtgctaaag cacatcaag 
      gcggggtac atggatctg aagacaagc atggaattg 
      ttggtgata gatgaagcc gatttggtt ttttctttt 
      ggttatgag agcgaggtg aaggagttg ttggagcgt 
      ttgccgagc atatatcag gctattttg gcctcggcc 
      acgttaagt gaagacgtg aaaaatctc aaaagttta 
      gtgttacac aatcctgta attttaaaa cttgaggaa 
      cctgaaatt gcgcctgca agccaactc agtcattac 
      cacttaatg gccgaggaa atggacaaa gccacgatt 
      ttatacgcc cttttaaaa ctgcactta attaggggg 
      aagaccatc atttttgtt aacaccgtc gataagtgt 
      tacaaaatt aaattgtac ttggaacag tttggaatt 
      ccaacctgt gttctaaac tccgagctt cctgcgaaa 
      atacggtgt cattcggta aatcagttc aatcagggc 
      atttatgac accatcgtg gcctcggac gaaaaagcg 
      ttggaacaa ccgggaaac ccgaacgac ccagagtta 
      aaaaaatca aaaaggaag aaggacaaa gaaagcggt 
      gtttctaga ggaatcgat tttcagtgc gttgccaat 
      gttattaat tttgatttt ccacttgac gtgcaatcc 
      tacgtgcat agagcggga agaacagct aggggaaac 
      aaccaaggc agtgtttta tcgtttgtg agcatacga 
      gaaaaacct ttgctagaa caggttgaa actcacttc 
      aaaagcgac caagatgat gtttcaatt ttcaagtcg 
      taccagttc aaattagac gaagttgag tcttttaaa 
      tatcgagca aaagacgca tggcgtgct gtaacgaga 
      attgcagta cgtgaagca agactgaag gaaatcaaa 
      caagagatt tttaattgt caaaaattg aaaagttac 
      tttgaggat aatccaact gatcttcaa gttttaagg 
      cacgataag gcgctgcat acggttaaa attcagcag 
      catttgtct gatgttccc gaatatatt gttccaccg 
      actttgaag ggtattgcc agcttaggg aagaagaaa 
      aggaaacgg gaatattat aggacatcg caagggtca 
      agcagcagg ttccaggcg aagaaaaac aatcctctg 
      ttaagcatg gactttgaa ggttttaac aaaaagaaa 
      aagaagaac tagtgattt tttgttgtt caatttgaa 
      ataaataaa ctttttatt gcatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY