| General information | |||
| TC001086 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10695800..10704063 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001086 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03134 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001086 RA
MMETDKIDD SNSNSQQYP MTILSPYDT ARKKEPSAT FNAERETCL KCFEKWSET DQVEFVENL LARMCHYQH GHINSYLKP MLQRDFISL LPKQGLDHV AENILSYLE ADSLRNAEL VCKEWFRVI SEGMLWKKL IERKVRTDS LWRGLAERR QWIQYLFKP RPGENHRPH SFYRALFPK IIRDIESIE SNWRNGRHI LQRINCRSE NSKGVYCLQ YDDQKIVSG LRDNTIKIW DRSTLQCTK VLIGHTGSV LCLQYDDKV IISGSSDST VRVWNVNTG EMVNTLIHH CEAVLHLRF NNGMMVTCS KDRSIAVWD MTSPNEITL RRVLVGHRA AVNVVDFDE KYIVSASGD RTIKVWNTS TCEFMRTLN GHKRGIACL QYRDRLVVS GSSDNTIRL WDIEGGACL RVLEGHEEL VRCIRFDSK RIVSGAYDG KIKVWDLVA ALDPRTPAS ALCLRTLVE HTGRVFRLQ FDEFQIVSS SHDDTILIW NFLNSIGER NTKSPSPSP FE
- >TC001086 RA
atgatggaa acagataaa attgacgat tcgaacagt aattcacaa cagtatcca atgacaata ttatcaccg tacgatacg gccaggaag aaagagcct tcggctact tttaacgcg gagagagaa acatgtctg aaatgcttc gaaaaatgg agcgaaacc gaccaagtc gaattcgta gaaaattta ctagctagg atgtgtcac taccaacac gggcacatc aattcctat ttgaaaccc atgctgcag cgagatttc atatctctt ttaccaaag caaggattg gaccacgtc gctgagaat atattgtca tatttggag gcggacagt ttacggaac gccgagttg gtctgcaaa gaatggttt agggtaatc tcagagggt atgctttgg aagaagcta atcgagcgg aaagttcgg acggattca ctgtggaga ggtttagcc gaacgcaga caatggata caatattta ttcaaacca cgacctggc gagaaccat cgtccccat tcgttctat cgagccctt ttcccgaaa atcatccgc gatatcgaa agcatagaa agtaattgg cggaacggt cggcacatc ttgcagcga atcaactgc cggtccgaa aatagcaaa ggggtttac tgtttgcag tacgacgat cagaaaatc gtgtcgggt ctcagggac aatactatt aaaatatgg gacagaagt acattgcag tgcactaaa gttcttata ggacatacg ggatcggtt ttgtgttta caatacgac gataaagtt attattagt ggttctagt gattcaact gtgcgcgtc tggaacgtt aacacggga gaaatggtc aatacgctc atacatcat tgcgaagcc gtccttcat cttagattc aataacgga atgatggtc acctgttca aaagatcgc tcgatagct gtgtgggac atgacatcg ccgaacgag atcaccctg cggcgggtg ctggtgggg cacagggcc gccgtgaac gtggtcgat ttcgacgag aaatacata gtttcggcg tcgggtgac cgaacgata aaagtgtgg aacacctca acttgtgag tttatgcga acgttaaac ggacataaa cgggggatc gcctgtttg caataccga gataggttg gtcgtaagc ggaagttcg gacaatacc ataaggttg tgggatatc gaaggtggc gcttgtctg cgggtgctt gaagggcac gaagagtta gtgcgttgt atccgtttc gattcgaaa cggattgtc agcggggct tatgatggg aaaattaaa gtttgggat ctcgttgcc gcgcttgat cccagaacg ccggccagt gctttatgt cttcgaaca ttggtagaa cacacaggt agagtattc aggttgcag tttgacgag tttcaaata gtgagcagt tcgcacgat gatacaatt cttatttgg aatttcctc aactcaatt ggagagaga aacacgaaa tcgccttcg ccctctccg tttgagtaa
- >TC001086 RA
tcagggttt ttgtttttg tccccagac tccttgcca gaatggtaa gctattttt ttgcccatt ctacaagtg aaatgggtt tgtgagctt tggttgttt gttctttta cactgtggc cccgaagta aagttgtaa atattcttt ctggtgttt tacggttgt tttaacgct tttatgtga aacaatgtg gcagtggta gtggtgcgg atgcaaaaa tcgcgaccg tgaggtgga cacaactcg cttcatcga tgatgatgg aaacagata aaattgacg attcgaaca gtaattcac aacagtatc caatgacaa tattatcac cgtacgata cggccagga agaaagagc cttcggcta cttttaacg cggagagag aaacatgtc tgaaatgct tcgaaaaat ggagcgaaa ccgaccaag tcgaattcg tagaaaatt tactagcta ggatgtgtc actaccaac acgggcaca tcaattcct atttgaaac ccatgctgc agcgagatt tcatatctc ttttaccaa agcaaggat tggaccacg tcgctgaga atatattgt catatttgg aggcggaca gtttacgga acgccgagt tggtctgca aagaatggt ttagggtaa tctcagagg gtatgcttt ggaagaagc taatcgagc ggaaagttc ggacggatt cactgtgga gaggtttag ccgaacgca gacaatgga tacaatatt tattcaaac cacgacctg gcgagaacc atcgtcccc attcgttct atcgagccc ttttcccga aaatcatcc gcgatatcg aaagcatag aaagtaatt ggcggaacg gtcggcaca tcttgcagc gaatcaact gccggtccg aaaatagca aaggggttt actgtttgc agtacgacg atcagaaaa tcgtgtcgg gtctcaggg acaatacta ttaaaatat gggacagaa gtacattgc agtgcacta aagttctta taggacata cgggatcgg ttttgtgtt tacaatacg acgataaag ttattatta gtggttcta gtgattcaa ctgtgcgcg tctggaacg ttaacacgg gagaaatgg tcaatacgc tcatacatc attgcgaag ccgtccttc atcttagat tcaataacg gaatgatgg tcacctgtt caaaagatc gctcgatag ctgtgtggg acatgacat cgccgaacg agatcaccc tgcggcggg tgctggtgg ggcacaggg ccgccgtga acgtggtcg atttcgacg agaaataca tagtttcgg cgtcgggtg accgaacga taaaagtgt ggaacacct caacttgtg agtttatgc gaacgttaa acggacata aacggggga tcgcctgtt tgcaatacc gagataggt tggtcgtaa gcggaagtt cggacaata ccataaggt tgtgggata tcgaaggtg gcgcttgtc tgcgggtgc ttgaagggc acgaagagt tagtgcgtt gtatccgtt tcgattcga aacggattg tcagcgggg cttatgatg ggaaaatta aagtttggg atctcgttg ccgcgcttg atcccagaa cgccggcca gtgctttat gtcttcgaa cattggtag aacacacag gtagagtat tcaggttgc agtttgacg agtttcaaa tagtgagca gttcgcacg atgatacaa ttcttattt ggaatttcc tcaactcaa ttggagaga gaaacacga aatcgcctt cgccctctc cgtttgagt aaattgtcc agaagccaa tttgtcatc atgtgtgtg tgagtgtgg aagagacaa ttttggagc aagtgctcg cataaactg actaggaat ataaaattt agaaaatta tttgtaata attgtgtat aatgttata tttaaattg gatatattt atgtcttaa tttataata tgaagcgta tttgacata attggagtt cgtttttta cgtgtttcg tctttatga aaaattttt gctgttgtg tttcctaga ccgaattaa gatgtcgac acaaaacct ctgttgatc tgttgtaac ctaaatgag tttaactac gaattatgt gatagtacg tttaaaaca tctaaagtg cggttttca tcgtgtgcg ccattaatc ttgttgacg ttttacatt tgttttatt tcgttatcg ttgaatttg caaaaatga acaactaat tttagctct ctagtctat gaaatttca atgataggc tacacacgt tattttagg aatcaagtt gttttaaaa ttctcacac tatagtgcg gtttctaca gtttgttgg gctttctag tctgtaatc attctttgc aaacttaga aagtgagtt tagtcggca aaaattgta tagtaagtt ttaaattta taggatttt ggggtgaat tcagtgatg attaatttt agtttagga ttattttac atcattttc atcaagttg gaaattgtg atctattat ttagggttc tccatattt attatggga aaatgattg caaccaaaa gtacttttt gttttgttt ggtgcatta atttaatga ggctgcaag tgtactggg attccaaga agtggccac gggctgttc ttgctattt attttagac gattctgcc aaaatgtag tatttctcc tagattaga tttacaagt aaacttata acggctttt gtgataaaa tcttacagt aaaatttgt atgcctgtt ttgtactat aactggaat gtacactat tcatgttaa aaaaaaaca ctatcaata aggcactaa atcatttta taggttaat gtaaatatt atgaatgta gtggtaaca tcatttgca tttactatt tttatttag agttattta tattttatg cgtgtaagt aatacattt tgctgagac aa
