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Gene information
General information
TC030998
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001081,TC001076
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5110525..5160975
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030998
    iBeetle screen iB_03132
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03133
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030998 RA
      MAPRRHLGQ VQGLLGIDP EDAGVTRCC VPTGECLRA 
      QQQLSPNNN DPMLIALDD LRDTVRVTC NNELCSSGR 
      YMHRECFDQ WEQTVLAYL KTCGRARSW SERQRHQNL 
      WTKKGYDLA YKACGCKCG RGHLKKDLD WMPPPPTSN 
      IFGRVEDIS EAVAGKKKK RRNRNSRPT LAISAAHAV 
      TNESRGRAG SLSSSTGSS SPPASGSEP SISPVHAHS 
      KKKSKVENS DKSRVGGGA NGIFTRRLD FSSFNALPK 
      NKLNSYQIK TEDEGNHGN DDTRCFILS TLASQGRSK 
      VHCVLCEET MPVFDRYPL VDGTFFLSP RQHAKGCIE 
      VKVEGRTQF LTAVCMACL EGVNGRAII CRFCNQRWD 
      GSSLVLGTM YSYDIFAAM PCCTERIKC NACYKPVLH 
      PAQRLNFFS DYSHIVPCP HCHTFDTHF VKPLAYCYA 
      RQPLRSLQM WP
    CDS sequence
    • >TC030998 RA
      atggccccg cgccgccac ctcggccaa gttcaaggc 
      ctcctgggc atcgatccc gaggacgcc ggcgtgacc 
      cggtgctgc gtgcccacc ggcgagtgc ctgcgcgcc 
      cagcagcag ctcagcccc aacaacaac gaccccatg 
      ctgatcgcc ctcgacgac ctccgcgac accgtgcgc 
      gtcacctgc aacaacgag ctctgctcc agcggcagg 
      tacatgcac cgggagtgc ttcgaccag tgggagcag 
      acggtgctg gcctacctc aagacctgc ggccgggcc 
      cgctcctgg tccgagcgc cagcgccac cagaacctc 
      tggaccaag aagggctac gacctcgcc tacaaggcc 
      tgcggctgc aagtgcggc cgcggacac ctcaaaaaa 
      gacttagac tggatgccg ccgccgccc accagcaac 
      atcttcgga cgcgtcgaa gacatctcc gaagccgtc 
      gccggcaaa aagaaaaaa cgcagaaac agaaattcc 
      aggcccacg ctggccatc tccgccgct cacgccgtc 
      accaatgaa agcagaggc agagccggc agtttgtcc 
      tccagcacg ggatcgtcc tcgccaccg gcttccggc 
      tccgaacct agtatatca ccggtacac gcgcactcc 
      aaaaagaag tccaaagtt gaaaattct gacaaaagc 
      cgagtcgga ggcggcgcc aatgggatt ttcacccgt 
      cgccttgat ttcagctcc ttcaacgcc ctgccgaag 
      aacaaactc aattcttat caaattaag acggaagac 
      gagggcaac cacggaaac gatgacact cgttgcttc 
      atcttgtca actcttgca tctcagggt cgctccaaa 
      gtgcactgt gtcctctgc gaagagacg atgccagtt 
      ttcgatagg tatcctcta gtcgatggc acatttttc 
      ctaagtccg cgccagcat gccaaaggc tgcatcgaa 
      gtgaaagta gagggacgc acccagttc ttgacagca 
      gtctgcatg gcctgcctt gaaggcgta aacggacgc 
      gcgattatc tgcaggttc tgcaaccag agatgggac 
      ggatcctct ctcgttctg ggtaccatg tactcctac 
      gatatcttc gctgccatg ccgtgctgc accgagcgc 
      atcaagtgc aacgcgtgc tacaagcca gtgctgcac 
      cccgcccag cgcctgaac ttcttcagc gactacagc 
      cacatagtg ccgtgtccg cattgccac acgttcgac 
      acgcacttc gtcaagcct ttggcgtac tgctacgcc 
      cgccagccg ctgcggtcg ctgcagatg tggccatag
    mRNA sequence
    • >TC030998 RA
      gcttccgaa agagccgct tgcaagacg tccatcgac 
      tcgtagacg tagacgcac ctcgcgcga tcctcctcc 
      cctcacgat ggccccgcg ccgccacct cggccaagt 
      tcaaggcct cctgggcat cgatcccga ggacgccgg 
      cgtgacccg gtgctgcgt gcccaccgg cgagtgcct 
      gcgcgccca gcagcagct cagccccaa caacaacga 
      ccccatgct gatcgccct cgacgacct ccgcgacac 
      cgtgcgcgt cacctgcaa caacgagct ctgctccag 
      cggcaggta catgcaccg ggagtgctt cgaccagtg 
      ggagcagac ggtgctggc ctacctcaa gacctgcgg 
      ccgggcccg ctcctggtc cgagcgcca gcgccacca 
      gaacctctg gaccaagaa gggctacga cctcgccta 
      caaggcctg cggctgcaa gtgcggccg cggacacct 
      caaaaaaga cttagactg gatgccgcc gccgcccac 
      cagcaacat cttcggacg cgtcgaaga catctccga 
      agccgtcgc cggcaaaaa gaaaaaacg cagaaacag 
      aaattccag gcccacgct ggccatctc cgccgctca 
      cgccgtcac caatgaaag cagaggcag agccggcag 
      tttgtcctc cagcacggg atcgtcctc gccaccggc 
      ttccggctc cgaacctag tatatcacc ggtacacgc 
      gcactccaa aaagaagtc caaagttga aaattctga 
      caaaagccg agtcggagg cggcgccaa tgggatttt 
      cacccgtcg ccttgattt cagctcctt caacgccct 
      gccgaagaa caaactcaa ttcttatca aattaagac 
      ggaagacga gggcaacca cggaaacga tgacactcg 
      ttgcttcat cttgtcaac tcttgcatc tcagggtcg 
      ctccaaagt gcactgtgt cctctgcga agagacgat 
      gccagtttt cgataggta tcctctagt cgatggcac 
      atttttcct aagtccgcg ccagcatgc caaaggctg 
      catcgaagt gaaagtaga gggacgcac ccagttctt 
      gacagcagt ctgcatggc ctgccttga aggcgtaaa 
      cggacgcgc gattatctg caggttctg caaccagag 
      atgggacgg atcctctct cgttctggg taccatgta 
      ctcctacga tatcttcgc tgccatgcc gtgctgcac 
      cgagcgcat caagtgcaa cgcgtgcta caagccagt 
      gctgcaccc cgcccagcg cctgaactt cttcagcga 
      ctacagcca catagtgcc gtgtccgca ttgccacac 
      gttcgacac gcacttcgt caagccttt ggcgtactg 
      ctacgcccg ccagccgct gcggtcgct gcagatgtg 
      gccatagcc cgcggcccc cgcctcgct cgcgaaggc 
      gccgccgac gccgccgtc gccgccccc agacctcat 
      ggacctgga tctggggct gctgtggcg cgagctggc 
      cggcttgcg tctcgacga gttctgccg tctgcaact 
      caccagagc gcggtagac ggacttaga tttctttgt 
      tatgttgtt gcattttat gtcgcattt atttttggg 
      gggcgcttt ccttctgga aacggccct cagacatat 
      tcgcgtgta aaaccgtta tgttattag tttattatt 
      aattatatg tttgttagg ttttagtga atttatatt 
      caacttaag tctttttat caaaggaat gacattgtg 
      atcatagtt atcttaatt tctagctcg aattcagtt 
      gacgctaaa gcaggtctt gttgaactg acagtgagt 
      gaagtttga aagcaatcc cgtttgagt ttttttttg 
      tctaattga tttgagttt tgttttttt tttattatt 
      ttttatttg aagtttagg ttaaagtta tgtagaggc 
      aactgtgtt ggttacgtt tacacggat cgtaacgat 
      cgctttatt acttaataa ctgtaatac ataatacta 
      tagtaataa aactgatat aattgctgt gtcccaata 
      attagtgat aggtgtgaa aggtgcggg accatggaa 
      tgggtctcg cgtgcgtgt cccgttatc gtcgttgtt 
      gttgtgatg ggttcaggg acagtcggt ctccctgcg 
      agtagcaca ctgccactt catcccgct gctgcaccc 
      gtagccttc cagaaacgg acgtacaaa gaactcagc 
      cgctcagat tatccgcga atacctctt tagacttga 
      tggtgatta tgatgaaga tggcggacc taggaaatt 
      gcttcaagt acaaaccgt tcgttactt ttgcaaatt 
      ttatataaa tgataacaa tataatcga ttgttgagg 
      gtaaaataa ttcctatct ccgtttagc caaagctta 
      agtccagca ggtggtgaa tttttctcc taccaattc 
      tcacactca tccaattat tcctactct ggctaaaac 
      tttccagtg gacaacctc tttacaaat atacaattt 
      tcgccacta actgatcaa gcctgtcca tgttagctg 
      actgggaga gccagtacc agaaaaatg cactacctc 
      ctggacttt tatgttgtt ttttgaaag tactacaca 
      tcgaccgac tagatatgt tgcgcgagt gcgagcgag 
      cttctgtaa cgtattgcg agcgtagca gtagtccct 
      tagcgtagt ggtaactgc atgccccca ctctaaacc 
      acccctaaa ccgcttctt gttatcgag gcctaactc 
      acggtctaa cttttctac gtgtaataa gaatccttg 
      aatgttgtc gttttttgg acaaaaact gtacataaa 
      cgtgttcga attcggaat ccattaagt ttttcaaat 
      tggtattat atgtcgatt aatcgtaag tctagctcg 
      tagtcgttt tttacaata aaatacgaa ttaataa