General information | |||
TC030998
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10617068..10667518 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030998 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03132 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03133 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC030998 RA
MAPRRHLGQ VQGLLGIDP EDAGVTRCC VPTGECLRA QQQLSPNNN DPMLIALDD LRDTVRVTC NNELCSSGR YMHRECFDQ WEQTVLAYL KTCGRARSW SERQRHQNL WTKKGYDLA YKACGCKCG RGHLKKDLD WMPPPPTSN IFGRVEDIS EAVAGKKKK RRNRNSRPT LAISAAHAV TNESRGRAG SLSSSTGSS SPPASGSEP SISPVHAHS KKKSKVENS DKSRVGGGA NGIFTRRLD FSSFNALPK NKLNSYQIK TEDEGNHGN DDTRCFILS TLASQGRSK VHCVLCEET MPVFDRYPL VDGTFFLSP RQHAKGCIE VKVEGRTQF LTAVCMACL EGVNGRAII CRFCNQRWD GSSLVLGTM YSYDIFAAM PCCTERIKC NACYKPVLH PAQRLNFFS DYSHIVPCP HCHTFDTHF VKPLAYCYA RQPLRSLQM WP
- >TC030998 RA
atggccccg cgccgccac ctcggccaa gttcaaggc ctcctgggc atcgatccc gaggacgcc ggcgtgacc cggtgctgc gtgcccacc ggcgagtgc ctgcgcgcc cagcagcag ctcagcccc aacaacaac gaccccatg ctgatcgcc ctcgacgac ctccgcgac accgtgcgc gtcacctgc aacaacgag ctctgctcc agcggcagg tacatgcac cgggagtgc ttcgaccag tgggagcag acggtgctg gcctacctc aagacctgc ggccgggcc cgctcctgg tccgagcgc cagcgccac cagaacctc tggaccaag aagggctac gacctcgcc tacaaggcc tgcggctgc aagtgcggc cgcggacac ctcaaaaaa gacttagac tggatgccg ccgccgccc accagcaac atcttcgga cgcgtcgaa gacatctcc gaagccgtc gccggcaaa aagaaaaaa cgcagaaac agaaattcc aggcccacg ctggccatc tccgccgct cacgccgtc accaatgaa agcagaggc agagccggc agtttgtcc tccagcacg ggatcgtcc tcgccaccg gcttccggc tccgaacct agtatatca ccggtacac gcgcactcc aaaaagaag tccaaagtt gaaaattct gacaaaagc cgagtcgga ggcggcgcc aatgggatt ttcacccgt cgccttgat ttcagctcc ttcaacgcc ctgccgaag aacaaactc aattcttat caaattaag acggaagac gagggcaac cacggaaac gatgacact cgttgcttc atcttgtca actcttgca tctcagggt cgctccaaa gtgcactgt gtcctctgc gaagagacg atgccagtt ttcgatagg tatcctcta gtcgatggc acatttttc ctaagtccg cgccagcat gccaaaggc tgcatcgaa gtgaaagta gagggacgc acccagttc ttgacagca gtctgcatg gcctgcctt gaaggcgta aacggacgc gcgattatc tgcaggttc tgcaaccag agatgggac ggatcctct ctcgttctg ggtaccatg tactcctac gatatcttc gctgccatg ccgtgctgc accgagcgc atcaagtgc aacgcgtgc tacaagcca gtgctgcac cccgcccag cgcctgaac ttcttcagc gactacagc cacatagtg ccgtgtccg cattgccac acgttcgac acgcacttc gtcaagcct ttggcgtac tgctacgcc cgccagccg ctgcggtcg ctgcagatg tggccatag
- >TC030998 RA
gcttccgaa agagccgct tgcaagacg tccatcgac tcgtagacg tagacgcac ctcgcgcga tcctcctcc cctcacgat ggccccgcg ccgccacct cggccaagt tcaaggcct cctgggcat cgatcccga ggacgccgg cgtgacccg gtgctgcgt gcccaccgg cgagtgcct gcgcgccca gcagcagct cagccccaa caacaacga ccccatgct gatcgccct cgacgacct ccgcgacac cgtgcgcgt cacctgcaa caacgagct ctgctccag cggcaggta catgcaccg ggagtgctt cgaccagtg ggagcagac ggtgctggc ctacctcaa gacctgcgg ccgggcccg ctcctggtc cgagcgcca gcgccacca gaacctctg gaccaagaa gggctacga cctcgccta caaggcctg cggctgcaa gtgcggccg cggacacct caaaaaaga cttagactg gatgccgcc gccgcccac cagcaacat cttcggacg cgtcgaaga catctccga agccgtcgc cggcaaaaa gaaaaaacg cagaaacag aaattccag gcccacgct ggccatctc cgccgctca cgccgtcac caatgaaag cagaggcag agccggcag tttgtcctc cagcacggg atcgtcctc gccaccggc ttccggctc cgaacctag tatatcacc ggtacacgc gcactccaa aaagaagtc caaagttga aaattctga caaaagccg agtcggagg cggcgccaa tgggatttt cacccgtcg ccttgattt cagctcctt caacgccct gccgaagaa caaactcaa ttcttatca aattaagac ggaagacga gggcaacca cggaaacga tgacactcg ttgcttcat cttgtcaac tcttgcatc tcagggtcg ctccaaagt gcactgtgt cctctgcga agagacgat gccagtttt cgataggta tcctctagt cgatggcac atttttcct aagtccgcg ccagcatgc caaaggctg catcgaagt gaaagtaga gggacgcac ccagttctt gacagcagt ctgcatggc ctgccttga aggcgtaaa cggacgcgc gattatctg caggttctg caaccagag atgggacgg atcctctct cgttctggg taccatgta ctcctacga tatcttcgc tgccatgcc gtgctgcac cgagcgcat caagtgcaa cgcgtgcta caagccagt gctgcaccc cgcccagcg cctgaactt cttcagcga ctacagcca catagtgcc gtgtccgca ttgccacac gttcgacac gcacttcgt caagccttt ggcgtactg ctacgcccg ccagccgct gcggtcgct gcagatgtg gccatagcc cgcggcccc cgcctcgct cgcgaaggc gccgccgac gccgccgtc gccgccccc agacctcat ggacctgga tctggggct gctgtggcg cgagctggc cggcttgcg tctcgacga gttctgccg tctgcaact caccagagc gcggtagac ggacttaga tttctttgt tatgttgtt gcattttat gtcgcattt atttttggg gggcgcttt ccttctgga aacggccct cagacatat tcgcgtgta aaaccgtta tgttattag tttattatt aattatatg tttgttagg ttttagtga atttatatt caacttaag tctttttat caaaggaat gacattgtg atcatagtt atcttaatt tctagctcg aattcagtt gacgctaaa gcaggtctt gttgaactg acagtgagt gaagtttga aagcaatcc cgtttgagt ttttttttg tctaattga tttgagttt tgttttttt tttattatt ttttatttg aagtttagg ttaaagtta tgtagaggc aactgtgtt ggttacgtt tacacggat cgtaacgat cgctttatt acttaataa ctgtaatac ataatacta tagtaataa aactgatat aattgctgt gtcccaata attagtgat aggtgtgaa aggtgcggg accatggaa tgggtctcg cgtgcgtgt cccgttatc gtcgttgtt gttgtgatg ggttcaggg acagtcggt ctccctgcg agtagcaca ctgccactt catcccgct gctgcaccc gtagccttc cagaaacgg acgtacaaa gaactcagc cgctcagat tatccgcga atacctctt tagacttga tggtgatta tgatgaaga tggcggacc taggaaatt gcttcaagt acaaaccgt tcgttactt ttgcaaatt ttatataaa tgataacaa tataatcga ttgttgagg gtaaaataa ttcctatct ccgtttagc caaagctta agtccagca ggtggtgaa tttttctcc taccaattc tcacactca tccaattat tcctactct ggctaaaac tttccagtg gacaacctc tttacaaat atacaattt tcgccacta actgatcaa gcctgtcca tgttagctg actgggaga gccagtacc agaaaaatg cactacctc ctggacttt tatgttgtt ttttgaaag tactacaca tcgaccgac tagatatgt tgcgcgagt gcgagcgag cttctgtaa cgtattgcg agcgtagca gtagtccct tagcgtagt ggtaactgc atgccccca ctctaaacc acccctaaa ccgcttctt gttatcgag gcctaactc acggtctaa cttttctac gtgtaataa gaatccttg aatgttgtc gttttttgg acaaaaact gtacataaa cgtgttcga attcggaat ccattaagt ttttcaaat tggtattat atgtcgatt aatcgtaag tctagctcg tagtcgttt tttacaata aaatacgaa ttaataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
