Status
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Gene information
General information
TC030998
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001081,TC001076 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10617068..10667518
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030998
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03132

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030998 RA
      MAPRRHLGQ VQGLLGIDP EDAGVTRCC VPTGECLRA 
QQQLSPNNN DPMLIALDD LRDTVRVTC NNELCSSGR 
YMHRECFDQ WEQTVLAYL KTCGRARSW SERQRHQNL 
WTKKGYDLA YKACGCKCG RGHLKKDLD WMPPPPTSN 
IFGRVEDIS EAVAGKKKK RRNRNSRPT LAISAAHAV 
TNESRGRAG SLSSSTGSS SPPASGSEP SISPVHAHS 
KKKSKVENS DKSRVGGGA NGIFTRRLD FSSFNALPK 
NKLNSYQIK TEDEGNHGN DDTRCFILS TLASQGRSK 
VHCVLCEET MPVFDRYPL VDGTFFLSP RQHAKGCIE 
VKVEGRTQF LTAVCMACL EGVNGRAII CRFCNQRWD 
GSSLVLGTM YSYDIFAAM PCCTERIKC NACYKPVLH 
PAQRLNFFS DYSHIVPCP HCHTFDTHF VKPLAYCYA 
RQPLRSLQM WP
    CDS sequence
    • >TC030998 RA
      atggccccg cgccgccac ctcggccaa gttcaaggc 
ctcctgggc atcgatccc gaggacgcc ggcgtgacc 
cggtgctgc gtgcccacc ggcgagtgc ctgcgcgcc 
cagcagcag ctcagcccc aacaacaac gaccccatg 
ctgatcgcc ctcgacgac ctccgcgac accgtgcgc 
gtcacctgc aacaacgag ctctgctcc agcggcagg 
tacatgcac cgggagtgc ttcgaccag tgggagcag 
acggtgctg gcctacctc aagacctgc ggccgggcc 
cgctcctgg tccgagcgc cagcgccac cagaacctc 
tggaccaag aagggctac gacctcgcc tacaaggcc 
tgcggctgc aagtgcggc cgcggacac ctcaaaaaa 
gacttagac tggatgccg ccgccgccc accagcaac 
atcttcgga cgcgtcgaa gacatctcc gaagccgtc 
gccggcaaa aagaaaaaa cgcagaaac agaaattcc 
aggcccacg ctggccatc tccgccgct cacgccgtc 
accaatgaa agcagaggc agagccggc agtttgtcc 
tccagcacg ggatcgtcc tcgccaccg gcttccggc 
tccgaacct agtatatca ccggtacac gcgcactcc 
aaaaagaag tccaaagtt gaaaattct gacaaaagc 
cgagtcgga ggcggcgcc aatgggatt ttcacccgt 
cgccttgat ttcagctcc ttcaacgcc ctgccgaag 
aacaaactc aattcttat caaattaag acggaagac 
gagggcaac cacggaaac gatgacact cgttgcttc 
atcttgtca actcttgca tctcagggt cgctccaaa 
gtgcactgt gtcctctgc gaagagacg atgccagtt 
ttcgatagg tatcctcta gtcgatggc acatttttc 
ctaagtccg cgccagcat gccaaaggc tgcatcgaa 
gtgaaagta gagggacgc acccagttc ttgacagca 
gtctgcatg gcctgcctt gaaggcgta aacggacgc 
gcgattatc tgcaggttc tgcaaccag agatgggac 
ggatcctct ctcgttctg ggtaccatg tactcctac 
gatatcttc gctgccatg ccgtgctgc accgagcgc 
atcaagtgc aacgcgtgc tacaagcca gtgctgcac 
cccgcccag cgcctgaac ttcttcagc gactacagc 
cacatagtg ccgtgtccg cattgccac acgttcgac 
acgcacttc gtcaagcct ttggcgtac tgctacgcc 
cgccagccg ctgcggtcg ctgcagatg tggccatag
    mRNA sequence
    • >TC030998 RA
      gcttccgaa agagccgct tgcaagacg tccatcgac 
tcgtagacg tagacgcac ctcgcgcga tcctcctcc 
cctcacgat ggccccgcg ccgccacct cggccaagt 
tcaaggcct cctgggcat cgatcccga ggacgccgg 
cgtgacccg gtgctgcgt gcccaccgg cgagtgcct 
gcgcgccca gcagcagct cagccccaa caacaacga 
ccccatgct gatcgccct cgacgacct ccgcgacac 
cgtgcgcgt cacctgcaa caacgagct ctgctccag 
cggcaggta catgcaccg ggagtgctt cgaccagtg 
ggagcagac ggtgctggc ctacctcaa gacctgcgg 
ccgggcccg ctcctggtc cgagcgcca gcgccacca 
gaacctctg gaccaagaa gggctacga cctcgccta 
caaggcctg cggctgcaa gtgcggccg cggacacct 
caaaaaaga cttagactg gatgccgcc gccgcccac 
cagcaacat cttcggacg cgtcgaaga catctccga 
agccgtcgc cggcaaaaa gaaaaaacg cagaaacag 
aaattccag gcccacgct ggccatctc cgccgctca 
cgccgtcac caatgaaag cagaggcag agccggcag 
tttgtcctc cagcacggg atcgtcctc gccaccggc 
ttccggctc cgaacctag tatatcacc ggtacacgc 
gcactccaa aaagaagtc caaagttga aaattctga 
caaaagccg agtcggagg cggcgccaa tgggatttt 
cacccgtcg ccttgattt cagctcctt caacgccct 
gccgaagaa caaactcaa ttcttatca aattaagac 
ggaagacga gggcaacca cggaaacga tgacactcg 
ttgcttcat cttgtcaac tcttgcatc tcagggtcg 
ctccaaagt gcactgtgt cctctgcga agagacgat 
gccagtttt cgataggta tcctctagt cgatggcac 
atttttcct aagtccgcg ccagcatgc caaaggctg 
catcgaagt gaaagtaga gggacgcac ccagttctt 
gacagcagt ctgcatggc ctgccttga aggcgtaaa 
cggacgcgc gattatctg caggttctg caaccagag 
atgggacgg atcctctct cgttctggg taccatgta 
ctcctacga tatcttcgc tgccatgcc gtgctgcac 
cgagcgcat caagtgcaa cgcgtgcta caagccagt 
gctgcaccc cgcccagcg cctgaactt cttcagcga 
ctacagcca catagtgcc gtgtccgca ttgccacac 
gttcgacac gcacttcgt caagccttt ggcgtactg 
ctacgcccg ccagccgct gcggtcgct gcagatgtg 
gccatagcc cgcggcccc cgcctcgct cgcgaaggc 
gccgccgac gccgccgtc gccgccccc agacctcat 
ggacctgga tctggggct gctgtggcg cgagctggc 
cggcttgcg tctcgacga gttctgccg tctgcaact 
caccagagc gcggtagac ggacttaga tttctttgt 
tatgttgtt gcattttat gtcgcattt atttttggg 
gggcgcttt ccttctgga aacggccct cagacatat 
tcgcgtgta aaaccgtta tgttattag tttattatt 
aattatatg tttgttagg ttttagtga atttatatt 
caacttaag tctttttat caaaggaat gacattgtg 
atcatagtt atcttaatt tctagctcg aattcagtt 
gacgctaaa gcaggtctt gttgaactg acagtgagt 
gaagtttga aagcaatcc cgtttgagt ttttttttg 
tctaattga tttgagttt tgttttttt tttattatt 
ttttatttg aagtttagg ttaaagtta tgtagaggc 
aactgtgtt ggttacgtt tacacggat cgtaacgat 
cgctttatt acttaataa ctgtaatac ataatacta 
tagtaataa aactgatat aattgctgt gtcccaata 
attagtgat aggtgtgaa aggtgcggg accatggaa 
tgggtctcg cgtgcgtgt cccgttatc gtcgttgtt 
gttgtgatg ggttcaggg acagtcggt ctccctgcg 
agtagcaca ctgccactt catcccgct gctgcaccc 
gtagccttc cagaaacgg acgtacaaa gaactcagc 
cgctcagat tatccgcga atacctctt tagacttga 
tggtgatta tgatgaaga tggcggacc taggaaatt 
gcttcaagt acaaaccgt tcgttactt ttgcaaatt 
ttatataaa tgataacaa tataatcga ttgttgagg 
gtaaaataa ttcctatct ccgtttagc caaagctta 
agtccagca ggtggtgaa tttttctcc taccaattc 
tcacactca tccaattat tcctactct ggctaaaac 
tttccagtg gacaacctc tttacaaat atacaattt 
tcgccacta actgatcaa gcctgtcca tgttagctg 
actgggaga gccagtacc agaaaaatg cactacctc 
ctggacttt tatgttgtt ttttgaaag tactacaca 
tcgaccgac tagatatgt tgcgcgagt gcgagcgag 
cttctgtaa cgtattgcg agcgtagca gtagtccct 
tagcgtagt ggtaactgc atgccccca ctctaaacc 
acccctaaa ccgcttctt gttatcgag gcctaactc 
acggtctaa cttttctac gtgtaataa gaatccttg 
aatgttgtc gttttttgg acaaaaact gtacataaa 
cgtgttcga attcggaat ccattaagt ttttcaaat 
tggtattat atgtcgatt aatcgtaag tctagctcg 
tagtcgttt tttacaata aaatacgaa ttaataa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY