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Gene information
General information
TC001075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5067737..5074261
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001075
    iBeetle screen iB_03131
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001075 RA
      MAEDSKSSD ASDSSSESP NEPVKLEKP IPKPLIQGK 
      VTKKRKNII SVCLTNCRY EVIRKIAAK FAYKEVSES 
      ENWNLYWTD LSITVERCK EMKRYQKIN HFPGMLEIC 
      RKDLLARNL NRMLRLFPK EYNFFPRTW CLPADLGEA 
      LTYSRLRRN RTFILKPDA GSQGRGIFV TKSLKDIRP 
      CDRGVCQVY INKPYLIDG YKFDLRIYT LITSCDPLR 
      IYIYEEGLV RFATTRYKE PNAVNITNV FMHLTNYAV 
      NKYSRTYNQ DTEEGSKRK LSWFNNYLR KLGHDVEGI 
      WKRIDDVII KTVISAAPV LKHSYAACF PNHDVIPAC 
      CELLGVDII LDKRLNPQI LEVNHSPSF HTDTALDWE 
      IKESLLSDM FAMMNLDKC DKRKVMRED RKRIRERLL 
      HGKDHQPPV VVSFDDYYR HEMNSRGGF RLVYPSQAT 
      KVHYNKFFD QGQGSLYSE TAASRAREA AQNALRDEM 
      QLKARLEAA KRVVIPPIK PTPPKKQES PNLSQKPTS 
      KSTAVLSVM SSSEPQMIV ESEERDRLQ KLAQREYLI 
      RSHGILEHV YFSMKKNGN LRPRDEKKF AIFDKIRAT 
      SENNFNKQN GIAISDFVE SIKAKSSI
    CDS sequence
    • >TC001075 RA
      atggctgaa gattccaaa agcagcgac gcctcagac 
      agttcctca gagagtccg aatgaaccc gttaaactc 
      gaaaaaccg ataccaaag cccctcatc caggggaaa 
      gtcacgaaa aaacggaaa aacatcatt tcagtgtgt 
      ctgacaaat tgccgctat gaagtcatt cggaaaatt 
      gctgccaaa tttgcgtac aaagaggtg agcgaaagc 
      gaaaactgg aacttgtac tggaccgac ctctccatc 
      acggtcgag cggtgcaag gaaatgaag cgctaccag 
      aaaatcaac cactttcct ggaatgctg gaaatttgc 
      cgcaaggat ctcctggca cggaatctg aacagaatg 
      ctcagactt tttccaaag gagtataat tttttccca 
      aggacttgg tgtctgcct gccgatttg ggcgaagcc 
      ctcacttat agtagactg cgtcggaac cggactttt 
      atactaaaa ccggacgcc gggagccaa ggacgcgga 
      attttcgta acgaaaagt ttgaaagac attaggccc 
      tgtgatagg ggcgtttgt caagtctac atcaacaaa 
      ccgtatctt atagatggt tacaagttc gacttgaga 
      atatacacc ttaatcaca tcgtgtgac cctttacga 
      atttatatt tacgaagag ggtttggtc agatttgct 
      accacaagg tacaaggaa cctaacgca gttaacatc 
      accaatgtc ttcatgcat ttgacaaac tatgcggtc 
      aataagtac agccgcacg tacaaccaa gacactgag 
      gaaggtagc aaaagaaaa ttgtcgtgg tttaacaac 
      tatttgcga aagttgggc cacgatgtt gagggtatt 
      tggaaaaga atcgacgat gttatcatt aaaactgtg 
      attagcgca gctcctgtt ctcaagcac agctacgct 
      gcttgtttc ccaaaccat gacgtgatc ccggcttgt 
      tgcgagcta ctaggagtc gatataatc ctggataaa 
      agactaaac ccccagatt ctagaagtg aaccactct 
      cctagtttc cacacagac actgctcta gactgggag 
      attaaagaa tctctcctt tcggacatg ttcgcaatg 
      atgaactta gacaagtgt gacaaaaga aaagtcatg 
      agagaagac cgaaaacgt ataagggaa agattacta 
      cacggtaaa gaccaccag cctccagtt gttgtcagc 
      tttgacgat tattaccgc cacgaaatg aacagtcgc 
      ggaggcttt cgcctagtt tacccaagc caggcaacc 
      aaagtacac tacaacaaa tttttcgat caaggccaa 
      ggctcctta tattccgaa actgcagct agtcgagcc 
      agagaagcg gctcagaat gctctccga gacgaaatg 
      cagctgaaa gcgaggttg gaagcagca aaacgagtg 
      gtgattcca ccaattaaa cccacaccg cccaaaaaa 
      caagaaagc ccgaatctg agtcaaaaa ccaacgtcc 
      aaatctacc gcagttttg tcggtaatg agttcctca 
      gaaccacaa atgatcgtc gaatcggag gaaagagat 
      cgcctccaa aagctggct cagcgggag tatcttatt 
      cgaagtcac ggtattttg gaacatgtc tatttttca 
      atgaagaag aatgggaat ttgagaccg cgtgacgaa 
      aagaagttc gcaattttt gacaagata cgcgccaca 
      agtgagaat aattttaac aagcaaaat gggatcgct 
      atctcggat tttgtggag tcgatcaag gccaagtcg 
      agcatctga
    mRNA sequence
    • >TC001075 RA
      ggtcttgag aatcctatt gtccgaaag tttgtccgg 
      aggagcaaa cccggttgt aaaaatttc gggccatta 
      ctgcattta atatgcgca ctgaaagct ctacggcct 
      ttatagtgg cggtttatg tacgtttta catccgcat 
      aaaatatca gaacaccat aaataagta gtcttgaca 
      attcctcaa atattacca ccgaaccaa cttagggga 
      taatttaca aactgaaat aaactgtcg caattttat 
      ttcttacgg cctgtacaa acacttaca atatttaat 
      agctagtaa caagattaa tgcttaata aaatcgaca 
      ctcaaacac taattatcg atctcttct ctgggacaa 
      cacactttg gggttggta ccattaaac ccgcactga 
      gaccgcatc aaaaacgtt agcgtttcg atagtaatc 
      ggagcattg aaattattt gaaagtgta aacacagtg 
      ggacattcc tgcaaatct atgcaccag ccgagttcc 
      gaattcggc gtcaaacac tcttctggc agcgctacc 
      aataaagaa agtcaaaga ttgatcaat tttccaaat 
      ttggccgta cctgtgcct gtttgcagt aaaacagca 
      gccgccaaa cacacaaac actgcacta ggtgtcaac 
      attgtcaag gagcaatca taactaaag caaccgttg 
      aagaaagta gtctgttcc cgttctcct tgacattgt 
      tatcggtcc gctcatcga ctccattaa gggtcatcg 
      cctcaaatt cattcacaa agtcattca gctcatttt 
      cgcaaccaa atccccgcc cgaaataaa tgacgttga 
      cagtttaat caaaattcg tgacccgat ttaatttcg 
      tcatcctcc tatggctga agattccaa aagcagcga 
      cgcctcaga cagttcctc agagagtcc gaatgaacc 
      cgttaaact cgaaaaacc gataccaaa gcccctcat 
      ccaggggaa agtcacgaa aaaacggaa aaacatcat 
      ttcagtgtg tctgacaaa ttgccgcta tgaagtcat 
      tcggaaaat tgctgccaa atttgcgta caaagaggt 
      gagcgaaag cgaaaactg gaacttgta ctggaccga 
      cctctccat cacggtcga gcggtgcaa ggaaatgaa 
      gcgctacca gaaaatcaa ccactttcc tggaatgct 
      ggaaatttg ccgcaagga tctcctggc acggaatct 
      gaacagaat gctcagact ttttccaaa ggagtataa 
      ttttttccc aaggacttg gtgtctgcc tgccgattt 
      gggcgaagc cctcactta tagtagact gcgtcggaa 
      ccggacttt tatactaaa accggacgc cgggagcca 
      aggacgcgg aattttcgt aacgaaaag tttgaaaga 
      cattaggcc ctgtgatag gggcgtttg tcaagtcta 
      catcaacaa accgtatct tatagatgg ttacaagtt 
      cgacttgag aatatacac cttaatcac atcgtgtga 
      ccctttacg aatttatat ttacgaaga gggtttggt 
      cagatttgc taccacaag gtacaagga acctaacgc 
      agttaacat caccaatgt cttcatgca tttgacaaa 
      ctatgcggt caataagta cagccgcac gtacaacca 
      agacactga ggaaggtag caaaagaaa attgtcgtg 
      gtttaacaa ctatttgcg aaagttggg ccacgatgt 
      tgagggtat ttggaaaag aatcgacga tgttatcat 
      taaaactgt gattagcgc agctcctgt tctcaagca 
      cagctacgc tgcttgttt cccaaacca tgacgtgat 
      cccggcttg ttgcgagct actaggagt cgatataat 
      cctggataa aagactaaa cccccagat tctagaagt 
      gaaccactc tcctagttt ccacacaga cactgctct 
      agactggga gattaaaga atctctcct ttcggacat 
      gttcgcaat gatgaactt agacaagtg tgacaaaag 
      aaaagtcat gagagaaga ccgaaaacg tataaggga 
      aagattact acacggtaa agaccacca gcctccagt 
      tgttgtcag ctttgacga ttattaccg ccacgaaat 
      gaacagtcg cggaggctt tcgcctagt ttacccaag 
      ccaggcaac caaagtaca ctacaacaa atttttcga 
      tcaaggcca aggctcctt atattccga aactgcagc 
      tagtcgagc cagagaagc ggctcagaa tgctctccg 
      agacgaaat gcagctgaa agcgaggtt ggaagcagc 
      aaaacgagt ggtgattcc accaattaa acccacacc 
      gcccaaaaa acaagaaag cccgaatct gagtcaaaa 
      accaacgtc caaatctac cgcagtttt gtcggtaat 
      gagttcctc agaaccaca aatgatcgt cgaatcgga 
      ggaaagaga tcgcctcca aaagctggc tcagcggga 
      gtatcttat tcgaagtca cggtatttt ggaacatgt 
      ctatttttc aatgaagaa gaatgggaa tttgagacc 
      gcgtgacga aaagaagtt cgcaatttt tgacaagat 
      acgcgccac aagtgagaa taattttaa caagcaaaa 
      tgggatcgc tatctcgga ttttgtgga gtcgatcaa 
      ggccaagtc gagcatctg atgagtgaa atgggcctc 
      taatacatt tattaacac acgtttatt tgcccagtt 
      gacaataat caaattatt ctcagtaat aatgtactt 
      ttatctat