| General information | |||
| TC001075 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10574280..10580804 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001075 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03131 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001075 RA
MAEDSKSSD ASDSSSESP NEPVKLEKP IPKPLIQGK VTKKRKNII SVCLTNCRY EVIRKIAAK FAYKEVSES ENWNLYWTD LSITVERCK EMKRYQKIN HFPGMLEIC RKDLLARNL NRMLRLFPK EYNFFPRTW CLPADLGEA LTYSRLRRN RTFILKPDA GSQGRGIFV TKSLKDIRP CDRGVCQVY INKPYLIDG YKFDLRIYT LITSCDPLR IYIYEEGLV RFATTRYKE PNAVNITNV FMHLTNYAV NKYSRTYNQ DTEEGSKRK LSWFNNYLR KLGHDVEGI WKRIDDVII KTVISAAPV LKHSYAACF PNHDVIPAC CELLGVDII LDKRLNPQI LEVNHSPSF HTDTALDWE IKESLLSDM FAMMNLDKC DKRKVMRED RKRIRERLL HGKDHQPPV VVSFDDYYR HEMNSRGGF RLVYPSQAT KVHYNKFFD QGQGSLYSE TAASRAREA AQNALRDEM QLKARLEAA KRVVIPPIK PTPPKKQES PNLSQKPTS KSTAVLSVM SSSEPQMIV ESEERDRLQ KLAQREYLI RSHGILEHV YFSMKKNGN LRPRDEKKF AIFDKIRAT SENNFNKQN GIAISDFVE SIKAKSSI
- >TC001075 RA
atggctgaa gattccaaa agcagcgac gcctcagac agttcctca gagagtccg aatgaaccc gttaaactc gaaaaaccg ataccaaag cccctcatc caggggaaa gtcacgaaa aaacggaaa aacatcatt tcagtgtgt ctgacaaat tgccgctat gaagtcatt cggaaaatt gctgccaaa tttgcgtac aaagaggtg agcgaaagc gaaaactgg aacttgtac tggaccgac ctctccatc acggtcgag cggtgcaag gaaatgaag cgctaccag aaaatcaac cactttcct ggaatgctg gaaatttgc cgcaaggat ctcctggca cggaatctg aacagaatg ctcagactt tttccaaag gagtataat tttttccca aggacttgg tgtctgcct gccgatttg ggcgaagcc ctcacttat agtagactg cgtcggaac cggactttt atactaaaa ccggacgcc gggagccaa ggacgcgga attttcgta acgaaaagt ttgaaagac attaggccc tgtgatagg ggcgtttgt caagtctac atcaacaaa ccgtatctt atagatggt tacaagttc gacttgaga atatacacc ttaatcaca tcgtgtgac cctttacga atttatatt tacgaagag ggtttggtc agatttgct accacaagg tacaaggaa cctaacgca gttaacatc accaatgtc ttcatgcat ttgacaaac tatgcggtc aataagtac agccgcacg tacaaccaa gacactgag gaaggtagc aaaagaaaa ttgtcgtgg tttaacaac tatttgcga aagttgggc cacgatgtt gagggtatt tggaaaaga atcgacgat gttatcatt aaaactgtg attagcgca gctcctgtt ctcaagcac agctacgct gcttgtttc ccaaaccat gacgtgatc ccggcttgt tgcgagcta ctaggagtc gatataatc ctggataaa agactaaac ccccagatt ctagaagtg aaccactct cctagtttc cacacagac actgctcta gactgggag attaaagaa tctctcctt tcggacatg ttcgcaatg atgaactta gacaagtgt gacaaaaga aaagtcatg agagaagac cgaaaacgt ataagggaa agattacta cacggtaaa gaccaccag cctccagtt gttgtcagc tttgacgat tattaccgc cacgaaatg aacagtcgc ggaggcttt cgcctagtt tacccaagc caggcaacc aaagtacac tacaacaaa tttttcgat caaggccaa ggctcctta tattccgaa actgcagct agtcgagcc agagaagcg gctcagaat gctctccga gacgaaatg cagctgaaa gcgaggttg gaagcagca aaacgagtg gtgattcca ccaattaaa cccacaccg cccaaaaaa caagaaagc ccgaatctg agtcaaaaa ccaacgtcc aaatctacc gcagttttg tcggtaatg agttcctca gaaccacaa atgatcgtc gaatcggag gaaagagat cgcctccaa aagctggct cagcgggag tatcttatt cgaagtcac ggtattttg gaacatgtc tatttttca atgaagaag aatgggaat ttgagaccg cgtgacgaa aagaagttc gcaattttt gacaagata cgcgccaca agtgagaat aattttaac aagcaaaat gggatcgct atctcggat tttgtggag tcgatcaag gccaagtcg agcatctga
- >TC001075 RA
ggtcttgag aatcctatt gtccgaaag tttgtccgg aggagcaaa cccggttgt aaaaatttc gggccatta ctgcattta atatgcgca ctgaaagct ctacggcct ttatagtgg cggtttatg tacgtttta catccgcat aaaatatca gaacaccat aaataagta gtcttgaca attcctcaa atattacca ccgaaccaa cttagggga taatttaca aactgaaat aaactgtcg caattttat ttcttacgg cctgtacaa acacttaca atatttaat agctagtaa caagattaa tgcttaata aaatcgaca ctcaaacac taattatcg atctcttct ctgggacaa cacactttg gggttggta ccattaaac ccgcactga gaccgcatc aaaaacgtt agcgtttcg atagtaatc ggagcattg aaattattt gaaagtgta aacacagtg ggacattcc tgcaaatct atgcaccag ccgagttcc gaattcggc gtcaaacac tcttctggc agcgctacc aataaagaa agtcaaaga ttgatcaat tttccaaat ttggccgta cctgtgcct gtttgcagt aaaacagca gccgccaaa cacacaaac actgcacta ggtgtcaac attgtcaag gagcaatca taactaaag caaccgttg aagaaagta gtctgttcc cgttctcct tgacattgt tatcggtcc gctcatcga ctccattaa gggtcatcg cctcaaatt cattcacaa agtcattca gctcatttt cgcaaccaa atccccgcc cgaaataaa tgacgttga cagtttaat caaaattcg tgacccgat ttaatttcg tcatcctcc tatggctga agattccaa aagcagcga cgcctcaga cagttcctc agagagtcc gaatgaacc cgttaaact cgaaaaacc gataccaaa gcccctcat ccaggggaa agtcacgaa aaaacggaa aaacatcat ttcagtgtg tctgacaaa ttgccgcta tgaagtcat tcggaaaat tgctgccaa atttgcgta caaagaggt gagcgaaag cgaaaactg gaacttgta ctggaccga cctctccat cacggtcga gcggtgcaa ggaaatgaa gcgctacca gaaaatcaa ccactttcc tggaatgct ggaaatttg ccgcaagga tctcctggc acggaatct gaacagaat gctcagact ttttccaaa ggagtataa ttttttccc aaggacttg gtgtctgcc tgccgattt gggcgaagc cctcactta tagtagact gcgtcggaa ccggacttt tatactaaa accggacgc cgggagcca aggacgcgg aattttcgt aacgaaaag tttgaaaga cattaggcc ctgtgatag gggcgtttg tcaagtcta catcaacaa accgtatct tatagatgg ttacaagtt cgacttgag aatatacac cttaatcac atcgtgtga ccctttacg aatttatat ttacgaaga gggtttggt cagatttgc taccacaag gtacaagga acctaacgc agttaacat caccaatgt cttcatgca tttgacaaa ctatgcggt caataagta cagccgcac gtacaacca agacactga ggaaggtag caaaagaaa attgtcgtg gtttaacaa ctatttgcg aaagttggg ccacgatgt tgagggtat ttggaaaag aatcgacga tgttatcat taaaactgt gattagcgc agctcctgt tctcaagca cagctacgc tgcttgttt cccaaacca tgacgtgat cccggcttg ttgcgagct actaggagt cgatataat cctggataa aagactaaa cccccagat tctagaagt gaaccactc tcctagttt ccacacaga cactgctct agactggga gattaaaga atctctcct ttcggacat gttcgcaat gatgaactt agacaagtg tgacaaaag aaaagtcat gagagaaga ccgaaaacg tataaggga aagattact acacggtaa agaccacca gcctccagt tgttgtcag ctttgacga ttattaccg ccacgaaat gaacagtcg cggaggctt tcgcctagt ttacccaag ccaggcaac caaagtaca ctacaacaa atttttcga tcaaggcca aggctcctt atattccga aactgcagc tagtcgagc cagagaagc ggctcagaa tgctctccg agacgaaat gcagctgaa agcgaggtt ggaagcagc aaaacgagt ggtgattcc accaattaa acccacacc gcccaaaaa acaagaaag cccgaatct gagtcaaaa accaacgtc caaatctac cgcagtttt gtcggtaat gagttcctc agaaccaca aatgatcgt cgaatcgga ggaaagaga tcgcctcca aaagctggc tcagcggga gtatcttat tcgaagtca cggtatttt ggaacatgt ctatttttc aatgaagaa gaatgggaa tttgagacc gcgtgacga aaagaagtt cgcaatttt tgacaagat acgcgccac aagtgagaa taattttaa caagcaaaa tgggatcgc tatctcgga ttttgtgga gtcgatcaa ggccaagtc gagcatctg atgagtgaa atgggcctc taatacatt tattaacac acgtttatt tgcccagtt gacaataat caaattatt ctcagtaat aatgtactt ttatctat
