Status
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Gene information
General information
TC032158
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001066,TC001065
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10448980..10476411
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032158
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03130

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032158 RA
      MPFVQRVVE PKFLSRTSL WDEEGKPLV SDDELEAVT 
      NNTLSNALR QLASLVLIA DDIFAELGS QLKEINKRS 
      EGLKAKIAA VEGKVSAFD PKKVTVPES DICTFALLK 
      NHYAAKCNP ETGLFTAST RPRALQSLY EAATKTPVH 
      LMRQLDRWR RDGHRSSRF FLCTPVLGN KRRKIRNKV 
      DIDIETRMP AAVQELRRW TSSEALGDI TVTPDCPNR 
      IAPNFTLTD GEATDGTAV SDDEGIDHK LPSPEEQLQ 
      VVALKFPAE IVAVDVSGR SFDRMSIQR RSLLHTDAN 
      AAKAEDPSL KRRTRTRRP RTRRRNTLA GTDQKEIRD 
      ALTAGEEPC SSANNHETE STTIGAVSR SKSSDLLRS 
      SNKKDSPDK KSHFNTLKQ WGKNRYDRF MNKSSESKS 
      EKKDDIDDF NIYETVTMK RKRNVDKDR RSHERNPSI 
      SSSEKSSVN LPVSSPLSS VMNIAVKLR ESSAQRRQR 
      RSGGSNKDE PPHSSSGNW SASSESGRA SIGSEFTST 
      TQPKSTTSA ATSNNSLNQ QAPNSANSR RRFVANTST 
      SSSITSEGT LTPDIIHDL HEDGETSSV YSCDTEGYY 
      TSFHMDSGL KTLKEEDLP CTPLHSTSA FSSSSNNTM 
      TAENEYELF GKGSTSTTT SSAGTVCTT LRASESNRS 
      LNGCPAVPE RKSSLSKHS NKDNSPESS LERDETSEK 
      TGTVKRSPA TNKASVVAM VHKQVNGDV SPDSGHNTS 
      SSPIESISS PNGVRSGSE FEFSESSDM EGPERIERI 
      RIKTTINSS RIPSMCVIT PPHSDDESI KSYNLNNFN 
      KRNLIDRRN ADRNKLDLN LKTSENEVN LLKSSDSDK 
      ENFTIQSRI INGTNKMQV INVNPQSGY ATVEVVDAN 
      ENIDVNKSS KVLRAPSPS GGTVTINET PGKSPPTQP 
      IIKATLLPF NNMFGRIKT NLANLTRRD SKSPNRNNQ 
      AISEEMGDS GEYVTLADV KNNNEKILT PKTETLYAN 
      DIVRKNLKF SGEKKRDTE YVSLDELPR NEDQTAASA 
      DNTNDSLER KRRQGARVT LDAEGKVVY SSDSLRRRK 
      GAHTTFEPG PFVKTTASP SQSPLPVHR LPKSVRPPQ 
      DSSRPLSPQ LGKLVIRAV PGMTSPTTE FIRIPPSTI 
      VAPSVRPTS PKAARGAYV HMQDTGKPA QDKGKPPLP 
      AQTAPHAQI SHDTVNRMN VINCSRKIV KRSDSYRIA 
      NTILSPAKM LSDKIDIED RIEKEIAAE LWRERINYP 
      PTVSPKCGR HIYTRVQAQ EILLASPNR VIIPPDSGE 
      HRARVLTAS VADTEIW
    CDS sequence
    • >TC032158 RA
      atgccgttc gtgcagcga gttgtcgag ccgaaattt 
      ttgagtcgg acgagtctc tgggacgaa gagggcaag 
      cctctggtc tccgacgat gagctggag gccgtcacc 
      aacaacacc ctgagcaat gccttgcgg caactcgcc 
      tccttggtg ctgatcgcc gatgacatc ttcgccgaa 
      ctgggcagt cagctcaag gagatcaac aagaggtcc 
      gagggtctg aaggcgaag atcgcggct gtggaggga 
      aaggtgtcg gcgtttgat cccaagaag gttacagtt 
      cctgaaagt gatatttgc actttcgcc ctactcaaa 
      aaccactac gctgccaaa tgcaacccc gagaccggg 
      ctcttcact gccagcacg cgtccccga gcgctccaa 
      agtctctat gaagccgcc acaaagact ccggtgcac 
      ctcatgcgc cagctggat agatggcgt cgcgacggt 
      catcggtct tcacgcttc ttcctgtgc acgccggtg 
      ttgggcaac aagagacgg aagatcagg aataaagtg 
      gacattgac attgagact cgcatgcct gcagccgtc 
      caagaattg cgcagatgg acttcaagc gaagccctc 
      ggtgacata acagtaact cctgattgt cctaatcga 
      attgcgccc aatttcact ctaacggac ggcgaagca 
      acggatgga actgcagtt tcagacgat gaaggcatt 
      gaccataag ctgccgtcg cctgaggaa caacttcaa 
      gtggtcgcg ctcaagttc cctgctgaa atcgtcgcg 
      gtggatgtt tcagggagg agctttgat cgaatgtcg 
      atacagaga aggtcgctt ttgcacacc gacgctaac 
      gcggcgaaa gcggaagat cccagtttg aaaagaagg 
      acgagaact agaagaccg aggactagg agaaggaac 
      acgcttgca ggaaccgac cagaaggag attagagac 
      gcgctgact gccggggag gagccatgc tcttcagct 
      aataatcat gagactgaa tccacaact atcggtgct 
      gtaagtcgc agtaaaagc agcgatctt ttacgttcg 
      tcaaacaaa aaagactcc cccgacaaa aaatcacac 
      tttaacact ctcaaacaa tggggcaag aaccgctac 
      gaccgcttc atgaacaag agttccgaa tcaaaatcg 
      gaaaagaaa gacgacatc gacgacttc aacatttac 
      gaaaccgta acaatgaaa cgcaaacgc aacgtcgac 
      aaagaccgt cgctcgcac gaacgcaac ccttcgatc 
      tcctcatcg gagaaatcc agcgtcaat ctccccgtc 
      tcaagcccc ctgtcttca gtgatgaac atcgccgtg 
      aaattacgc gagagttcc gctcaaaga cggcagcga 
      agatccggt ggcagtaac aaagacgaa cctccgcat 
      tcctcaagt ggaaattgg agcgcaagc tcggaaagc 
      ggccgagct tccattggc tccgaattc acaagcacc 
      actcagccg aaatccacc acatcggcc gcaacatca 
      aacaactct ctcaaccaa caagcgccc aattctgca 
      aacagcaga cggcgattt gttgcaaac acgtcaacc 
      tcaagcagt attacgagc gaaggcact ctcacgccc 
      gacattatc cacgacctg cacgaagac ggcgaaacc 
      agctccgtc tattcgtgc gatacggaa ggttactac 
      acgtccttc cacatggac agcgggctc aagaccctc 
      aaagaggag gacctcccg tgcactccc ttacattcc 
      acctccgcc ttttcctcc agttcgaac aacacaatg 
      acggcggaa aacgaatac gaactcttc ggcaaaggc 
      tccacctcg accaccacc agctcggct ggcacagtc 
      tgcacgact ttgcgcgcc tctgagagc aatcgatcc 
      ctaaatgga tgtcctgca gtccccgaa agaaaaagc 
      tccttatcg aaacactcc aataaggat aattcgccg 
      gaaagttcc ctcgaacgt gacgaaact agcgaaaaa 
      accggaacc gtgaaacga agtccggcg acaaacaag 
      gcaagtgtc gtcgcaatg gtccacaaa caagtcaac 
      ggggacgta tcccccgac agtggccat aacacctcc 
      agctcaccg atcgaatca atcagcagc cccaacggg 
      gtcaggagt ggatcagaa ttcgagttt tccgaatct 
      tccgacatg gaaggtccc gaaagaatc gaaagaatc 
      cgcataaaa actacaatt aattcgagt agaatacct 
      tcgatgtgt gtgattaca ccgccgcat agcgacgac 
      gaaagtatc aaaagctac aatttgaat aatttcaat 
      aaacggaac ttgattgat aggaggaat gcagacaga 
      aacaagcta gatttgaat ttgaaaaca agcgagaac 
      gaagtgaat ttgttaaaa tcgagcgac tcggataag 
      gagaatttt acaattcaa agccggatt attaacggg 
      acgaacaaa atgcaagtt attaatgtt aatccccag 
      tcgggttac gccacggtt gaagtggtc gatgccaac 
      gaaaacata gacgttaac aaaagtagt aaagtttta 
      agagcaccg tcacccagt ggcggcact gtcactatt 
      aacgaaaca ccggggaaa tcacccccg acacaacca 
      ataatcaaa gcgactctt cttccgttt aataacatg 
      tttggaaga attaaaacc aacttagct aatctcact 
      cgacgcgat agcaagtcg cctaatagg aacaatcag 
      gcaattagt gaggagatg ggtgactca ggcgaatac 
      gtaacacta gccgacgtt aaaaataac aatgagaag 
      attttaact ccgaaaact gaaacactt tatgcgaac 
      gatattgtg cgaaaaaat ctgaaattc agtggagag 
      aagaaaagg gacaccgaa tacgtgtct cttgacgag 
      ctccctcgt aatgaggac caaaccgcc gcttccgcg 
      gacaataca aacgactct cttgagaga aagagacgt 
      caaggtgca cgtgtaact ctagacgca gagggtaaa 
      gtggtctac agttcggac agtctccgg aggaggaaa 
      ggggcccat acaacattc gagcccggt ccattcgta 
      aaaaccaca gctagtccc tctcaaagc ccgcttccc 
      gtgcaccgg ctccccaaa tcagtgcgt cctccccaa 
      gactcgagc cgtccgctg tcgccccag ctgggcaag 
      ctagtgatc cgggccgtt ccggggatg acatcgccg 
      acgaccgag tttatccgg atcccgccc tcgaccatc 
      gtggccccc agcgtgcgc cccacgtcc ccgaaggcc 
      gcccgcggg gcctacgtc cacatgcaa gacaccgga 
      aagcctgct caagacaaa ggtaagcct ccgctcccc 
      gcccaaacc gccccccat gcccaaatc tcacacgac 
      acagtcaat cgaatgaat gttattaac tgttcgcgg 
      aaaattgtc aaacgcagc gacagttac cggattgcg 
      aatacgatc ctatcgccg gcgaaaatg ctcagcgat 
      aagatcgat atcgaggac cggatcgag aaggaaatt 
      gcggcggag ttgtggcgc gaacggata aattacccg 
      ccgacggtt tcgcccaag tgcggacgg cacatctac 
      actcgggtt caggcgcag gagatcctg ctggcgtcc 
      ccgaatcgg gtcattatc ccgcccgat agcggggag 
      catcgcgct cgcgttctt acagcctcc gttgccgac 
      acggaaatt tggtga
    mRNA sequence
    • >TC032158 RA
      tttgtttgc cggcaatct gacagtgag gtagctgcg 
      cgtccgcaa ggcgcgtat cttcggatt caaaaaatg 
      gaaaacttg gtaaagact cacagaatg tgatagtgc 
      gcgaactga gtgaaaatg aagtgttgg tgaaaatgc 
      cgttcgtgc agcgagttg tcgagccga aatttttga 
      gtcggacga gtctctggg acgaagagg gcaagcctc 
      tggtctccg acgatgagc tggaggccg tcaccaaca 
      acaccctga gcaatgcct tgcggcaac tcgcctcct 
      tggtgctga tcgccgatg acatcttcg ccgaactgg 
      gcagtcagc tcaaggaga tcaacaaga ggtccgagg 
      gtctgaagg cgaagatcg cggctgtgg agggaaagg 
      tgtcggcgt ttgatccca agaaggtta cagttcctg 
      aaagtgata tttgcactt tcgccctac tcaaaaacc 
      actacgctg ccaaatgca accccgaga ccgggctct 
      tcactgcca gcacgcgtc cccgagcgc tccaaagtc 
      tctatgaag ccgccacaa agactccgg tgcacctca 
      tgcgccagc tggatagat ggcgtcgcg acggtcatc 
      ggtcttcac gcttcttcc tgtgcacgc cggtgttgg 
      gcaacaaga gacggaaga tcaggaata aagtggaca 
      ttgacattg agactcgca tgcctgcag ccgtccaag 
      aattgcgca gatggactt caagcgaag ccctcggtg 
      acataacag taactcctg attgtccta atcgaattg 
      cgcccaatt tcactctaa cggacggcg aagcaacgg 
      atggaactg cagtttcag acgatgaag gcattgacc 
      ataagctgc cgtcgcctg aggaacaac ttcaagtgg 
      tcgcgctca agttccctg ctgaaatcg tcgcggtgg 
      atgtttcag ggaggagct ttgatcgaa tgtcgatac 
      agagaaggt cgcttttgc acaccgacg ctaacgcgg 
      cgaaagcgg aagatccca gtttgaaaa gaaggacga 
      gaactagaa gaccgagga ctaggagaa ggaacacgc 
      ttgcaggaa ccgaccaga aggagatta gagacgcgc 
      tgactgccg gggaggagc catgctctt cagctaata 
      atcatgaga ctgaatcca caactatcg gtgctgtaa 
      gtcgcagta aaagcagcg atcttttac gttcgtcaa 
      acaaaaaag actcccccg acaaaaaat cacacttta 
      acactctca aacaatggg gcaagaacc gctacgacc 
      gcttcatga acaagagtt ccgaatcaa aatcggaaa 
      agaaagacg acatcgacg acttcaaca tttacgaaa 
      ccgtaacaa tgaaacgca aacgcaacg tcgacaaag 
      accgtcgct cgcacgaac gcaaccctt cgatctcct 
      catcggaga aatccagcg tcaatctcc ccgtctcaa 
      gccccctgt cttcagtga tgaacatcg ccgtgaaat 
      tacgcgaga gttccgctc aaagacggc agcgaagat 
      ccggtggca gtaacaaag acgaacctc cgcattcct 
      caagtggaa attggagcg caagctcgg aaagcggcc 
      gagcttcca ttggctccg aattcacaa gcaccactc 
      agccgaaat ccaccacat cggccgcaa catcaaaca 
      actctctca accaacaag cgcccaatt ctgcaaaca 
      gcagacggc gatttgttg caaacacgt caacctcaa 
      gcagtatta cgagcgaag gcactctca cgcccgaca 
      ttatccacg acctgcacg aagacggcg aaaccagct 
      ccgtctatt cgtgcgata cggaaggtt actacacgt 
      ccttccaca tggacagcg ggctcaaga ccctcaaag 
      aggaggacc tcccgtgca ctcccttac attccacct 
      ccgcctttt cctccagtt cgaacaaca caatgacgg 
      cggaaaacg aatacgaac tcttcggca aaggctcca 
      cctcgacca ccaccagct cggctggca cagtctgca 
      cgactttgc gcgcctctg agagcaatc gatccctaa 
      atggatgtc ctgcagtcc ccgaaagaa aaagctcct 
      tatcgaaac actccaata aggataatt cgccggaaa 
      gttccctcg aacgtgacg aaactagcg aaaaaaccg 
      gaaccgtga aacgaagtc cggcgacaa acaaggcaa 
      gtgtcgtcg caatggtcc acaaacaag tcaacgggg 
      acgtatccc ccgacagtg gccataaca cctccagct 
      caccgatcg aatcaatca gcagcccca acggggtca 
      ggagtggat cagaattcg agttttccg aatcttccg 
      acatggaag gtcccgaaa gaatcgaaa gaatccgca 
      taaaaacta caattaatt cgagtagaa taccttcga 
      tgtgtgtga ttacaccgc cgcatagcg acgacgaaa 
      gtatcaaaa gctacaatt tgaataatt tcaataaac 
      ggaacttga ttgatagga ggaatgcag acagaaaca 
      agctagatt tgaatttga aaacaagcg agaacgaag 
      tgaatttgt taaaatcga gcgactcgg ataaggaga 
      attttacaa ttcaaagcc ggattatta acgggacga 
      acaaaatgc aagttatta atgttaatc cccagtcgg 
      gttacgcca cggttgaag tggtcgatg ccaacgaaa 
      acatagacg ttaacaaaa gtagtaaag ttttaagag 
      caccgtcac ccagtggcg gcactgtca ctattaacg 
      aaacaccgg ggaaatcac ccccgacac aaccaataa 
      tcaaagcga ctcttcttc cgtttaata acatgtttg 
      gaagaatta aaaccaact tagctaatc tcactcgac 
      gcgatagca agtcgccta ataggaaca atcaggcaa 
      ttagtgagg agatgggtg actcaggcg aatacgtaa 
      cactagccg acgttaaaa ataacaatg agaagattt 
      taactccga aaactgaaa cactttatg cgaacgata 
      ttgtgcgaa aaaatctga aattcagtg gagagaaga 
      aaagggaca ccgaatacg tgtctcttg acgagctcc 
      ctcgtaatg aggaccaaa ccgccgctt ccgcggaca 
      atacaaacg actctcttg agagaaaga gacgtcaag 
      gtgcacgtg taactctag acgcagagg gtaaagtgg 
      tctacagtt cggacagtc tccggagga ggaaagggg 
      cccatacaa cattcgagc ccggtccat tcgtaaaaa 
      ccacagcta gtccctctc aaagcccgc ttcccgtgc 
      accggctcc ccaaatcag tgcgtcctc cccaagact 
      cgagccgtc cgctgtcgc cccagctgg gcaagctag 
      tgatccggg ccgttccgg ggatgacat cgccgacga 
      ccgagttta tccggatcc cgccctcga ccatcgtgg 
      cccccagcg tgcgcccca cgtccccga aggccgccc 
      gcggggcct acgtccaca tgcaagaca ccggaaagc 
      ctgctcaag acaaaggta agcctccgc tccccgccc 
      aaaccgccc cccatgccc aaatctcac acgacacag 
      tcaatcgaa tgaatgtta ttaactgtt cgcggaaaa 
      ttgtcaaac gcagcgaca gttaccgga ttgcgaata 
      cgatcctat cgccggcga aaatgctca gcgataaga 
      tcgatatcg aggaccgga tcgagaagg aaattgcgg 
      cggagttgt ggcgcgaac ggataaatt acccgccga 
      cggtttcgc ccaagtgcg gacggcaca tctacactc 
      gggttcagg cgcaggaga tcctgctgg cgtccccga 
      atcgggtca ttatcccgc ccgatagcg gggagcatc 
      gcgctcgcg ttcttacag cctccgttg ccgacacgg 
      aaatttggt gaaaatcga tgagacacg caccatgtt 
      agctggtag tgggttcct tctgtccat atttattcc 
      ctgctttgg tgattggcc atattcatg attaacgcc 
      ttggtggta gagctataa ccaggaata tggctgatt 
      tattcacgt gggagaagt gtatgtaaa cattttgtg 
      cacacacgc tgtcatgtc ttccatagt tttagacct 
      tcttcctga gtgagtttt gtgatataa ttgtttaag 
      actgaaggc atcttcgga cagtctcct aaaaataca 
      tggctgctc gaaaatgcc ccgttttta tgtatattt 
      gtcatttgc acgatctgt gtcttggtg tacataact 
      tagattatt tttgcgatt gtttcgcgt caggggcgg 
      attagagac accacccac gtgtttgat taaaaattt 
      gtttggaga aactttcag aaaatcgca tttattagc 
      tcattgttt caaatttaa atccttttg cttcgattt 
      tgcttttgc ttttttgta taatcacga ttattcaat 
      cccatatta cgaaaattg tatttgctt ggagttttt 
      aagtgctct ccactttga gtaaaatcg aaccgcgta 
      attaatatt ctcgagtac tcgcagact gaactataa 
      cgatttaag cttttctgg tttctaata gttgggttt 
      attatccgc taatttaat ttttgggaa gtgctctta 
      gtccgccgc tgtttcttg tgcattatt ttttgtttg 
      taaatacat agctataga gtgcatgca atatgatta 
      tgtgttatt tttatgtgt acaactgca ttaaatgta 
      tgtatgttg a
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