| General information | |||
| TC032158
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10448980..10476411 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032158 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03130 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032158 RA
MPFVQRVVE PKFLSRTSL WDEEGKPLV SDDELEAVT NNTLSNALR QLASLVLIA DDIFAELGS QLKEINKRS EGLKAKIAA VEGKVSAFD PKKVTVPES DICTFALLK NHYAAKCNP ETGLFTAST RPRALQSLY EAATKTPVH LMRQLDRWR RDGHRSSRF FLCTPVLGN KRRKIRNKV DIDIETRMP AAVQELRRW TSSEALGDI TVTPDCPNR IAPNFTLTD GEATDGTAV SDDEGIDHK LPSPEEQLQ VVALKFPAE IVAVDVSGR SFDRMSIQR RSLLHTDAN AAKAEDPSL KRRTRTRRP RTRRRNTLA GTDQKEIRD ALTAGEEPC SSANNHETE STTIGAVSR SKSSDLLRS SNKKDSPDK KSHFNTLKQ WGKNRYDRF MNKSSESKS EKKDDIDDF NIYETVTMK RKRNVDKDR RSHERNPSI SSSEKSSVN LPVSSPLSS VMNIAVKLR ESSAQRRQR RSGGSNKDE PPHSSSGNW SASSESGRA SIGSEFTST TQPKSTTSA ATSNNSLNQ QAPNSANSR RRFVANTST SSSITSEGT LTPDIIHDL HEDGETSSV YSCDTEGYY TSFHMDSGL KTLKEEDLP CTPLHSTSA FSSSSNNTM TAENEYELF GKGSTSTTT SSAGTVCTT LRASESNRS LNGCPAVPE RKSSLSKHS NKDNSPESS LERDETSEK TGTVKRSPA TNKASVVAM VHKQVNGDV SPDSGHNTS SSPIESISS PNGVRSGSE FEFSESSDM EGPERIERI RIKTTINSS RIPSMCVIT PPHSDDESI KSYNLNNFN KRNLIDRRN ADRNKLDLN LKTSENEVN LLKSSDSDK ENFTIQSRI INGTNKMQV INVNPQSGY ATVEVVDAN ENIDVNKSS KVLRAPSPS GGTVTINET PGKSPPTQP IIKATLLPF NNMFGRIKT NLANLTRRD SKSPNRNNQ AISEEMGDS GEYVTLADV KNNNEKILT PKTETLYAN DIVRKNLKF SGEKKRDTE YVSLDELPR NEDQTAASA DNTNDSLER KRRQGARVT LDAEGKVVY SSDSLRRRK GAHTTFEPG PFVKTTASP SQSPLPVHR LPKSVRPPQ DSSRPLSPQ LGKLVIRAV PGMTSPTTE FIRIPPSTI VAPSVRPTS PKAARGAYV HMQDTGKPA QDKGKPPLP AQTAPHAQI SHDTVNRMN VINCSRKIV KRSDSYRIA NTILSPAKM LSDKIDIED RIEKEIAAE LWRERINYP PTVSPKCGR HIYTRVQAQ EILLASPNR VIIPPDSGE HRARVLTAS VADTEIW
- >TC032158 RA
atgccgttc gtgcagcga gttgtcgag ccgaaattt ttgagtcgg acgagtctc tgggacgaa gagggcaag cctctggtc tccgacgat gagctggag gccgtcacc aacaacacc ctgagcaat gccttgcgg caactcgcc tccttggtg ctgatcgcc gatgacatc ttcgccgaa ctgggcagt cagctcaag gagatcaac aagaggtcc gagggtctg aaggcgaag atcgcggct gtggaggga aaggtgtcg gcgtttgat cccaagaag gttacagtt cctgaaagt gatatttgc actttcgcc ctactcaaa aaccactac gctgccaaa tgcaacccc gagaccggg ctcttcact gccagcacg cgtccccga gcgctccaa agtctctat gaagccgcc acaaagact ccggtgcac ctcatgcgc cagctggat agatggcgt cgcgacggt catcggtct tcacgcttc ttcctgtgc acgccggtg ttgggcaac aagagacgg aagatcagg aataaagtg gacattgac attgagact cgcatgcct gcagccgtc caagaattg cgcagatgg acttcaagc gaagccctc ggtgacata acagtaact cctgattgt cctaatcga attgcgccc aatttcact ctaacggac ggcgaagca acggatgga actgcagtt tcagacgat gaaggcatt gaccataag ctgccgtcg cctgaggaa caacttcaa gtggtcgcg ctcaagttc cctgctgaa atcgtcgcg gtggatgtt tcagggagg agctttgat cgaatgtcg atacagaga aggtcgctt ttgcacacc gacgctaac gcggcgaaa gcggaagat cccagtttg aaaagaagg acgagaact agaagaccg aggactagg agaaggaac acgcttgca ggaaccgac cagaaggag attagagac gcgctgact gccggggag gagccatgc tcttcagct aataatcat gagactgaa tccacaact atcggtgct gtaagtcgc agtaaaagc agcgatctt ttacgttcg tcaaacaaa aaagactcc cccgacaaa aaatcacac tttaacact ctcaaacaa tggggcaag aaccgctac gaccgcttc atgaacaag agttccgaa tcaaaatcg gaaaagaaa gacgacatc gacgacttc aacatttac gaaaccgta acaatgaaa cgcaaacgc aacgtcgac aaagaccgt cgctcgcac gaacgcaac ccttcgatc tcctcatcg gagaaatcc agcgtcaat ctccccgtc tcaagcccc ctgtcttca gtgatgaac atcgccgtg aaattacgc gagagttcc gctcaaaga cggcagcga agatccggt ggcagtaac aaagacgaa cctccgcat tcctcaagt ggaaattgg agcgcaagc tcggaaagc ggccgagct tccattggc tccgaattc acaagcacc actcagccg aaatccacc acatcggcc gcaacatca aacaactct ctcaaccaa caagcgccc aattctgca aacagcaga cggcgattt gttgcaaac acgtcaacc tcaagcagt attacgagc gaaggcact ctcacgccc gacattatc cacgacctg cacgaagac ggcgaaacc agctccgtc tattcgtgc gatacggaa ggttactac acgtccttc cacatggac agcgggctc aagaccctc aaagaggag gacctcccg tgcactccc ttacattcc acctccgcc ttttcctcc agttcgaac aacacaatg acggcggaa aacgaatac gaactcttc ggcaaaggc tccacctcg accaccacc agctcggct ggcacagtc tgcacgact ttgcgcgcc tctgagagc aatcgatcc ctaaatgga tgtcctgca gtccccgaa agaaaaagc tccttatcg aaacactcc aataaggat aattcgccg gaaagttcc ctcgaacgt gacgaaact agcgaaaaa accggaacc gtgaaacga agtccggcg acaaacaag gcaagtgtc gtcgcaatg gtccacaaa caagtcaac ggggacgta tcccccgac agtggccat aacacctcc agctcaccg atcgaatca atcagcagc cccaacggg gtcaggagt ggatcagaa ttcgagttt tccgaatct tccgacatg gaaggtccc gaaagaatc gaaagaatc cgcataaaa actacaatt aattcgagt agaatacct tcgatgtgt gtgattaca ccgccgcat agcgacgac gaaagtatc aaaagctac aatttgaat aatttcaat aaacggaac ttgattgat aggaggaat gcagacaga aacaagcta gatttgaat ttgaaaaca agcgagaac gaagtgaat ttgttaaaa tcgagcgac tcggataag gagaatttt acaattcaa agccggatt attaacggg acgaacaaa atgcaagtt attaatgtt aatccccag tcgggttac gccacggtt gaagtggtc gatgccaac gaaaacata gacgttaac aaaagtagt aaagtttta agagcaccg tcacccagt ggcggcact gtcactatt aacgaaaca ccggggaaa tcacccccg acacaacca ataatcaaa gcgactctt cttccgttt aataacatg tttggaaga attaaaacc aacttagct aatctcact cgacgcgat agcaagtcg cctaatagg aacaatcag gcaattagt gaggagatg ggtgactca ggcgaatac gtaacacta gccgacgtt aaaaataac aatgagaag attttaact ccgaaaact gaaacactt tatgcgaac gatattgtg cgaaaaaat ctgaaattc agtggagag aagaaaagg gacaccgaa tacgtgtct cttgacgag ctccctcgt aatgaggac caaaccgcc gcttccgcg gacaataca aacgactct cttgagaga aagagacgt caaggtgca cgtgtaact ctagacgca gagggtaaa gtggtctac agttcggac agtctccgg aggaggaaa ggggcccat acaacattc gagcccggt ccattcgta aaaaccaca gctagtccc tctcaaagc ccgcttccc gtgcaccgg ctccccaaa tcagtgcgt cctccccaa gactcgagc cgtccgctg tcgccccag ctgggcaag ctagtgatc cgggccgtt ccggggatg acatcgccg acgaccgag tttatccgg atcccgccc tcgaccatc gtggccccc agcgtgcgc cccacgtcc ccgaaggcc gcccgcggg gcctacgtc cacatgcaa gacaccgga aagcctgct caagacaaa ggtaagcct ccgctcccc gcccaaacc gccccccat gcccaaatc tcacacgac acagtcaat cgaatgaat gttattaac tgttcgcgg aaaattgtc aaacgcagc gacagttac cggattgcg aatacgatc ctatcgccg gcgaaaatg ctcagcgat aagatcgat atcgaggac cggatcgag aaggaaatt gcggcggag ttgtggcgc gaacggata aattacccg ccgacggtt tcgcccaag tgcggacgg cacatctac actcgggtt caggcgcag gagatcctg ctggcgtcc ccgaatcgg gtcattatc ccgcccgat agcggggag catcgcgct cgcgttctt acagcctcc gttgccgac acggaaatt tggtga
- >TC032158 RA
tttgtttgc cggcaatct gacagtgag gtagctgcg cgtccgcaa ggcgcgtat cttcggatt caaaaaatg gaaaacttg gtaaagact cacagaatg tgatagtgc gcgaactga gtgaaaatg aagtgttgg tgaaaatgc cgttcgtgc agcgagttg tcgagccga aatttttga gtcggacga gtctctggg acgaagagg gcaagcctc tggtctccg acgatgagc tggaggccg tcaccaaca acaccctga gcaatgcct tgcggcaac tcgcctcct tggtgctga tcgccgatg acatcttcg ccgaactgg gcagtcagc tcaaggaga tcaacaaga ggtccgagg gtctgaagg cgaagatcg cggctgtgg agggaaagg tgtcggcgt ttgatccca agaaggtta cagttcctg aaagtgata tttgcactt tcgccctac tcaaaaacc actacgctg ccaaatgca accccgaga ccgggctct tcactgcca gcacgcgtc cccgagcgc tccaaagtc tctatgaag ccgccacaa agactccgg tgcacctca tgcgccagc tggatagat ggcgtcgcg acggtcatc ggtcttcac gcttcttcc tgtgcacgc cggtgttgg gcaacaaga gacggaaga tcaggaata aagtggaca ttgacattg agactcgca tgcctgcag ccgtccaag aattgcgca gatggactt caagcgaag ccctcggtg acataacag taactcctg attgtccta atcgaattg cgcccaatt tcactctaa cggacggcg aagcaacgg atggaactg cagtttcag acgatgaag gcattgacc ataagctgc cgtcgcctg aggaacaac ttcaagtgg tcgcgctca agttccctg ctgaaatcg tcgcggtgg atgtttcag ggaggagct ttgatcgaa tgtcgatac agagaaggt cgcttttgc acaccgacg ctaacgcgg cgaaagcgg aagatccca gtttgaaaa gaaggacga gaactagaa gaccgagga ctaggagaa ggaacacgc ttgcaggaa ccgaccaga aggagatta gagacgcgc tgactgccg gggaggagc catgctctt cagctaata atcatgaga ctgaatcca caactatcg gtgctgtaa gtcgcagta aaagcagcg atcttttac gttcgtcaa acaaaaaag actcccccg acaaaaaat cacacttta acactctca aacaatggg gcaagaacc gctacgacc gcttcatga acaagagtt ccgaatcaa aatcggaaa agaaagacg acatcgacg acttcaaca tttacgaaa ccgtaacaa tgaaacgca aacgcaacg tcgacaaag accgtcgct cgcacgaac gcaaccctt cgatctcct catcggaga aatccagcg tcaatctcc ccgtctcaa gccccctgt cttcagtga tgaacatcg ccgtgaaat tacgcgaga gttccgctc aaagacggc agcgaagat ccggtggca gtaacaaag acgaacctc cgcattcct caagtggaa attggagcg caagctcgg aaagcggcc gagcttcca ttggctccg aattcacaa gcaccactc agccgaaat ccaccacat cggccgcaa catcaaaca actctctca accaacaag cgcccaatt ctgcaaaca gcagacggc gatttgttg caaacacgt caacctcaa gcagtatta cgagcgaag gcactctca cgcccgaca ttatccacg acctgcacg aagacggcg aaaccagct ccgtctatt cgtgcgata cggaaggtt actacacgt ccttccaca tggacagcg ggctcaaga ccctcaaag aggaggacc tcccgtgca ctcccttac attccacct ccgcctttt cctccagtt cgaacaaca caatgacgg cggaaaacg aatacgaac tcttcggca aaggctcca cctcgacca ccaccagct cggctggca cagtctgca cgactttgc gcgcctctg agagcaatc gatccctaa atggatgtc ctgcagtcc ccgaaagaa aaagctcct tatcgaaac actccaata aggataatt cgccggaaa gttccctcg aacgtgacg aaactagcg aaaaaaccg gaaccgtga aacgaagtc cggcgacaa acaaggcaa gtgtcgtcg caatggtcc acaaacaag tcaacgggg acgtatccc ccgacagtg gccataaca cctccagct caccgatcg aatcaatca gcagcccca acggggtca ggagtggat cagaattcg agttttccg aatcttccg acatggaag gtcccgaaa gaatcgaaa gaatccgca taaaaacta caattaatt cgagtagaa taccttcga tgtgtgtga ttacaccgc cgcatagcg acgacgaaa gtatcaaaa gctacaatt tgaataatt tcaataaac ggaacttga ttgatagga ggaatgcag acagaaaca agctagatt tgaatttga aaacaagcg agaacgaag tgaatttgt taaaatcga gcgactcgg ataaggaga attttacaa ttcaaagcc ggattatta acgggacga acaaaatgc aagttatta atgttaatc cccagtcgg gttacgcca cggttgaag tggtcgatg ccaacgaaa acatagacg ttaacaaaa gtagtaaag ttttaagag caccgtcac ccagtggcg gcactgtca ctattaacg aaacaccgg ggaaatcac ccccgacac aaccaataa tcaaagcga ctcttcttc cgtttaata acatgtttg gaagaatta aaaccaact tagctaatc tcactcgac gcgatagca agtcgccta ataggaaca atcaggcaa ttagtgagg agatgggtg actcaggcg aatacgtaa cactagccg acgttaaaa ataacaatg agaagattt taactccga aaactgaaa cactttatg cgaacgata ttgtgcgaa aaaatctga aattcagtg gagagaaga aaagggaca ccgaatacg tgtctcttg acgagctcc ctcgtaatg aggaccaaa ccgccgctt ccgcggaca atacaaacg actctcttg agagaaaga gacgtcaag gtgcacgtg taactctag acgcagagg gtaaagtgg tctacagtt cggacagtc tccggagga ggaaagggg cccatacaa cattcgagc ccggtccat tcgtaaaaa ccacagcta gtccctctc aaagcccgc ttcccgtgc accggctcc ccaaatcag tgcgtcctc cccaagact cgagccgtc cgctgtcgc cccagctgg gcaagctag tgatccggg ccgttccgg ggatgacat cgccgacga ccgagttta tccggatcc cgccctcga ccatcgtgg cccccagcg tgcgcccca cgtccccga aggccgccc gcggggcct acgtccaca tgcaagaca ccggaaagc ctgctcaag acaaaggta agcctccgc tccccgccc aaaccgccc cccatgccc aaatctcac acgacacag tcaatcgaa tgaatgtta ttaactgtt cgcggaaaa ttgtcaaac gcagcgaca gttaccgga ttgcgaata cgatcctat cgccggcga aaatgctca gcgataaga tcgatatcg aggaccgga tcgagaagg aaattgcgg cggagttgt ggcgcgaac ggataaatt acccgccga cggtttcgc ccaagtgcg gacggcaca tctacactc gggttcagg cgcaggaga tcctgctgg cgtccccga atcgggtca ttatcccgc ccgatagcg gggagcatc gcgctcgcg ttcttacag cctccgttg ccgacacgg aaatttggt gaaaatcga tgagacacg caccatgtt agctggtag tgggttcct tctgtccat atttattcc ctgctttgg tgattggcc atattcatg attaacgcc ttggtggta gagctataa ccaggaata tggctgatt tattcacgt gggagaagt gtatgtaaa cattttgtg cacacacgc tgtcatgtc ttccatagt tttagacct tcttcctga gtgagtttt gtgatataa ttgtttaag actgaaggc atcttcgga cagtctcct aaaaataca tggctgctc gaaaatgcc ccgttttta tgtatattt gtcatttgc acgatctgt gtcttggtg tacataact tagattatt tttgcgatt gtttcgcgt caggggcgg attagagac accacccac gtgtttgat taaaaattt gtttggaga aactttcag aaaatcgca tttattagc tcattgttt caaatttaa atccttttg cttcgattt tgcttttgc ttttttgta taatcacga ttattcaat cccatatta cgaaaattg tatttgctt ggagttttt aagtgctct ccactttga gtaaaatcg aaccgcgta attaatatt ctcgagtac tcgcagact gaactataa cgatttaag cttttctgg tttctaata gttgggttt attatccgc taatttaat ttttgggaa gtgctctta gtccgccgc tgtttcttg tgcattatt ttttgtttg taaatacat agctataga gtgcatgca atatgatta tgtgttatt tttatgtgt acaactgca ttaaatgta tgtatgttg a
