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Gene information
General information
TC001064 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4932637..4941912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001064
    iBeetle screen iB_03129
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001064 RA
      MTLVVTILV PGSTNLVGK FTQSVRRIV 
      QDVKDEGTA SGQTKEEVI ETNERLRAV 
      RIRLDESYD TAKKALVTL MGKYNDSKS 
      VHNIFQRYN LLKAMIKEV IRLETQYWT 
      LVDIPKQEK QETVPAFVL RACAIMEKT 
      QKSGEGVKT SAKLAEEAQ DKRERIERL 
      ENMTTSQIE AENTQMTND LYRLLKKYS 
      GLRNLIRQL KIEYTNAKI YPMVLRYTM 
      LKDMIKDIM LHPDYMEVC HEVDA
    CDS sequence
    • >TC001064 RA
      atgacactt gtcgtaacg atccttgtt ccaggctcg 
      acaaacttg gtgggtaaa ttcacgcaa agtgtgcga 
      aggatcgtc caggacgtc aaggacgag ggaactgcg 
      agtggtcaa acgaaggaa gaggtcata gaaactaat 
      gaaagactc cgtgctgta agaatacgt ctggatgag 
      tcttatgac acggcgaaa aaagcactt gttactctc 
      atgggaaaa tataacgac agcaagagt gttcacaat 
      atcttccag agatataac ttgttgaag gcgatgatt 
      aaggaagtg atacggtta gaaacccaa tattggaca 
      ttagtcgat attccgaag caagaaaaa caggaaaca 
      gtacccgcc tttgttctc agggcttgc gccattatg 
      gaaaaaaca cagaaatcc ggcgaaggt gtaaaaact 
      tcggcaaag ctcgccgaa gaagcgcag gataaaagg 
      gagagaata gaacgtctt gagaatatg acgacttcg 
      caaatagaa gctgaaaac acacaaatg actaacgat 
      ctctatagg ctattgaag aaatattcc ggtctccgg 
      aacctcatt cggcagtta aaaatcgag tacacaaac 
      gcgaaaatc tacccgatg gtcctccgt tacacgatg 
      ctgaaggac atgatcaaa gacatcatg ctccacccc 
      gactacatg gaggtgtgc cacgaggtg gacgcatag
    mRNA sequence
    • >TC001064 RA
      ctatagatg agacgccta ccttacgag atgttttgt 
      aaataaaac caccgtatc ataacatca catcacgaa 
      gattcccca ggtgtgtat aggaaatgg taacacact 
      acacctgac gacaatcag cagttcagt gacgtgcaa 
      acagagact tctgccccc tctgtgaca ccgctggtg 
      ggatttatg acacttgtc gtaacgatc cttgttcca 
      ggctcgaca aacttggtg ggtaaattc acgcaaagt 
      gtgcgaagg atcgtccag gacgtcaag gacgaggga 
      actgcgagt ggtcaaacg aaggaagag gtcatagaa 
      actaatgaa agactccgt gctgtaaga atacgtctg 
      gatgagtct tatgacacg gcgaaaaaa gcacttgtt 
      actctcatg ggaaaatat aacgacagc aagagtgtt 
      cacaatatc ttccagaga tataacttg ttgaaggcg 
      atgattaag gaagtgata cggttagaa acccaatat 
      tggacatta gtcgatatt ccgaagcaa gaaaaacag 
      gaaacagta cccgccttt gttctcagg gcttgcgcc 
      attatggaa aaaacacag aaatccggc gaaggtgta 
      aaaacttcg gcaaagctc gccgaagaa gcgcaggat 
      aaaagggag agaatagaa cgtcttgag aatatgacg 
      acttcgcaa atagaagct gaaaacaca caaatgact 
      aacgatctc tataggcta ttgaagaaa tattccggt 
      ctccggaac ctcattcgg cagttaaaa atcgagtac 
      acaaacgcg aaaatctac ccgatggtc ctccgttac 
      acgatgctg aaggacatg atcaaagac atcatgctc 
      caccccgac tacatggag gtgtgccac gaggtggac 
      gcatagccc aagtcgttc tcatagata ggatatcgt 
      cgtcggagc ctcagatcg taacagtct cactccatc 
      aaccaggaa attgagaaa ttgtcactt tggtgggaa 
      acgctattt cgtgattgc aatcgactc ccatttgga 
      gatgtgtcg gcaaaatta tgtctaaaa gtcgcttat 
      ttgtggatt acgtgttaa gtgagatgt gatgattcg 
      agaattggg aaaaaaccg attgactct aggccccaa 
      attcagata ctgcgagct ttgttatca tcatcaatt 
      aaaatggtc gattgtttc gttgaaaac ggggcttca 
      aattcactg ccactagac gggaatccg tgtcgaaca 
      gtattgcat tccttaata caaaagagt ggtgcttat 
      tgaagcaat taaattatt tggatcatt atgaactgt 
      tttctcatt aactgcaga agcgatcgt ttttgtaat 
      gaatgaccc aagactgaa gtatttaat taaagacct 
      tcgtcaaac actccttac ctacctttg atctcattc 
      cgaaaatcg atgtttgca atatcgcac ttgttttta 
      tttttagtc gcattgtct agcttgcga tgtgccaaa 
      caaatctcg tcacggaaa ttaaaatac gcccaaaaa 
      ttgctttga tcctaacaa atttggcga aacgtggcg 
      actgattaa tgaaactgc taataaaaa tctactgtt 
      ctacaccta atcctctat aaggagatt aatattgta 
      cctatgaaa aattaacgt gccagaatg catttaatg 
      tgtataata ttctggatt gatgtgatg tcttcttgt 
      aacttttgt agaccccaa ccaatctct aaaatagcg 
      tactcgtta tgtaacaat aggtaatag atgatagga 
      catttgggt gcccggtag agacatccg tttacaaga 
      atcgtctaa aacaccaat ttgttaatc gattgtttc 
      tcgtagatt tagattttt tactttctt tctaacatt 
      atctgtgtc aaaatgaca acattagta atagcttta 
      agaaacgta cttataggc tagttattt tgtaagact 
      tttttctag ttttctatc atttcgcct acgtagaaa 
      acgatagct gtatgtctt gttgtagca cgatagggt 
      tccacttcg ttacttgac ggtgttttg ctaagtccc 
      tcatgaagt tcgttattt cctcggtaa agtacaaat 
      ttatttaaa tgaacagac acgcttaaa gtacacccc 
      ttgtttcgc ctatggact tgtcaatgt attactaac 
      gcaaagaag atggttgtt tatatgtga tgatgtatg 
      tgtaaaaat atatttaag agataaata ttccatgtt 
      aaaatatta cagggtttg ttgacgctt tctttttcg 
      tttcgtttt taaatgttt taactataa tgtgcttaa 
      gtaagctga tgaggtttt tttacataa tcattaacg 
      ttacgaatt tatcgtgaa atatcaaat taaaagtaa 
      tatcgccct gttgaatat catattaat attgttata 
      agggaacaa taatattta ttcaagaat tataacaag 
      attttattt tgtatattg tcgcatata attatgcaa 
      aactatata atatactgt atatgtgta c