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Gene information
General information
TC001053 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4724678..4725592
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001053
    iBeetle screen iB_03126
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 27.27% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001053 RA
      MHYCQVCFI VLQLLVSVF GWSMDRYDN 
      RVLRSPVTV LRHRRTQME VRTNQCMFN 
      ICEEVPLYP EDKIRAMIE ENQSLHSYF 
      GSSVIEPSS FDSGISIVS RNNAVMEPF 
      CKTRDVSST PKLMMDVDH VERSIANTN 
      KYKQVIKYQ ICDFEEGES CFPGSILNL 
      KTRCLQKFN VIKLMTANL DSNTLEYRK 
      FEIPSTCVC TYTKEA
    CDS sequence
    • >TC001053 RA
      atgcattat tgccaagtg tgttttatt gtgcttcag 
      cttctcgtc tcagtcttc ggctggtct atggaccgc 
      tacgacaat cgggttttg cgaagccct gttaccgtt 
      ctccgacac agaagaacc caaatggaa gttagaaca 
      aaccaatgt atgtttaat atttgcgaa gaagttcct 
      ttatacccc gaagacaaa atacgtgcg atgattgaa 
      gaaaatcag tctctacat tcttacttc ggaagcagc 
      gttatagaa ccttcaagc tttgactcg ggtatttcg 
      atcgtatca aggaacaat gcggtaatg gaacctttt 
      tgtaaaact agggatgta tcgtctacg ccgaaatta 
      atgatggac gttgatcat gttgaacga tcgattgct 
      aacacaaac aagtacaaa caagtcata aaatatcaa 
      atctgcgat tttgaagag ggagagagc tgttttccg 
      ggatctatc ctcaatttg aagaccaga tgtttgcaa 
      aagttcaac gtcattaag ctcatgacc gcaaatttg 
      gactctaac actttggag tatcgaaag tttgaaata 
      cccagtact tgtgtttgc acctacact aaggaagcc 
      taa
    mRNA sequence
    • >TC001053 RA
      aatctcacg tgaaggaag ttggtttgt taatttcaa 
      gtaaaactg agccatcat gcattattg ccaagtgtg 
      ttttattgt gcttcagct tctcgtctc agtcttcgg 
      ctggtctat ggaccgcta cgacaatcg ggttttgcg 
      aagccctgt taccgttct ccgacacag aagaaccca 
      aatggaagt tagaacaaa ccaatgtat gtttaatat 
      ttgcgaaga agttccttt ataccccga agacaaaat 
      acgtgcgat gattgaaga aaatcagtc tctacattc 
      ttacttcgg aagcagcgt tatagaacc ttcaagctt 
      tgactcggg tatttcgat cgtatcaag gaacaatgc 
      ggtaatgga acctttttg taaaactag ggatgtatc 
      gtctacgcc gaaattaat gatggacgt tgatcatgt 
      tgaacgatc gattgctaa cacaaacaa gtacaaaca 
      agtcataaa atatcaaat ctgcgattt tgaagaggg 
      agagagctg ttttccggg atctatcct caatttgaa 
      gaccagatg tttgcaaaa gttcaacgt cattaagct 
      catgaccgc aaatttgga ctctaacac tttggagta 
      tcgaaagtt tgaaatacc cagtacttg tgtttgcac 
      ctacactaa ggaagccta acaattttt gccattttt 
      ttatttttg taaaataat atcttactg aatgtattt 
      gtaccccaa ataataaaa taatcaaat agta