Status
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Gene information
General information
TC030997
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001044,TC001045,TC001046
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10163176..10181980
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030997
    iBeetle screen iB_03125

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Half of the larvae on the slide are stage 2 larvae.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030997 RA
      MLKWTVMAR READKSDAL RRPTADAST SETTPKSNK 
      TKRRHSSGH RRSGGRRSS LGLEMDKEN QFTSTPIKS 
      FEDHHQFDA LRDVSNLTP NRRVLSAKK CRRSASPSS 
      HRKHKKKKL CLNPSHRKE FGEGKNYFR PFETVDSHI 
      EGVLVDKDL SGELRVKRR IQDTYAPTL PTFTTEYSP 
      CFMRDAHCL YNFTPAKAT PLGKYLALD QDTPHPSKK 
      PKIDHVSDF LHQINFITS PEDDKLTCP TMESKSTLT 
      LREESKVSP LVKKLVDLR FSQLSCDKD TCKNNSSLI 
      NDLSLDQIV DAILNSSAE SAANKENES NETHEENLI 
      NTENERHVA TSFDRCSSD SGFKSSTTE YSHQLEGNF 
      QCKCKTSTP NAALCDKTI ININETFNE RCVDVINPR 
      KRPSSTPND DNNAKRAYL DKSEEDNVY CTLKRQRCI 
      RRRRTDDEK KMSAKKSRK VDEPIIGDE SFDSNISGK 
      IAEDNSTPL NETFTLLTP VDNSRRFRR CLLFESPTS 
      LSESASTTN SSLRDVRGT MDLNIRCEN DELYANIIR 
      CKDLYRPNG KQINAYVKV ALSDRLGDN RRKRNGILQ 
      RTAVQADSS KPFFNHTFK FPLQKESLQ KRLHIEVWH 
      RDRSSRTSE FLGCMSFDV RHVYTKDIS GSYRLLPQS 
      SGRCQNIPI SVEPLDREA EEDCQPIMC ESQSSVDEI 
      MSLDGLDND LKKATNKAI LSEQQKYAD ENLFLRYLE 
      LDPTEGPEA IPAAMQRKA TGNKNGRTP FTQTKKLTR 
      APKSGFGFS VVWTHPPRI ERVEKGLPA EKAGILPGD 
      YIIFVDKHN VVMMPEIDI LNLIRSYGS QLTLEIFRR 
      NAAKNGSVP SVRRLNSTG TCSTTGLPI SSNLVQRRP 
      STVCSTNTA SVDYNRRKL HLPQVTFSA EKASNNQEE 
      NRTKAMYQL IHKEQQYAT GLQFAVTRF VSALAERRD 
      LITPSEHKI LFQNSEEIL RVTEDILDN LVHEEGVHN 
      LIRTYHAKL NEITAAYRR YCSGIKKAD CVLANKTKN 
      SNSEFVRFL HTPSIPRRR PDITAFIHK PLEHYREVL 
      KAFTVIQSH TKPNHEDYP VINQLVHDL QHTYREITV 
      EAGLMEPMG EGRPLLSVQ DLENRLVFT KCKPFVLNK 
      PGRQWIFGG DLGRVEGRN VRQYWTLLF SDLLLFAKV 
      SRDRVLFII EDPLPLAHI TDMFFNVRK KDTEFRITV 
      TPGGRLASS PTVHCGPDL SRTPKKNTG KRTVILRAP 
      TTELKAVWQ NLLQRQIFH VNAGMDGSS FSSPLESPD 
      APITSSVAT LQSAESLSF RRQVRLCSG HCSEPVDNS 
      CLPAQNRSP RHHQPSLPT QTSLTKTPD TPSANISSQ 
      SSSEQSQAF TSHFTSESS LVPKYYLPE EPQSPSAIL 
      TPSTEISSD ADAHNSEDI DFLKFDEDP WDITNFDYD 
      LSSLNIDGL STKKMQKQI DALIEHKCK QLSKNNSGK 
      GNAIHLEKW MKGHLDSDI ADEAELEPL SEEWTEEML 
      RKRSEELQL IDAQGNVTS RNDTRRIES RCEDNNLSD 
      HDQSPSKST TTESQVTVR SSPLVPETV PVCRQCHKN 
      CLPSVNNSV KSARSNNNT ENPKKDEND NKDEDDWRP 
      LLLMGLSAI NPAASLVKL DPFSTPIPQ INVAPPTPE 
      SSTKTGSTS DQKQKDGSC SCRNSRPEL NNLELSNEV 
      DISPDDSPQ TEEHPYHSL SSSNLTLRR YGTVSSLER 
      LGTDEHEDN WDERYSSDE ENEERGGID NEAFYHSSI 
      KSWTAKAGS FVAEKMSFF ERLGEDYRS TGRFFERYL 
      KTAETTVNG DDVQEDETS GATSGEEIW GTPTSGGEM 
      EDPLSSPNY EGKQSPYDG SISSDNGDD TELMMDELL 
      MTPPITGAI MRGLLPRRT LEPLIEEDF SESCSPSSS 
      GTPTEPSMS PEQGNGTGD VADKCSTAV ENRIETPEM 
      GSAVTTSEA TAVPKIHRS ESYRKIVEA AEEDYDDAL 
      GFFDRFKPT VKFINIERV PRAKSAKIF EFFNMRRER 
      RIHQTIPEG KQLIKIFSK EGTISDNVP TSYAEKPLS 
      PRRLKDKQM DRRFWKQLS RRRGNKMSN LPA
    • >TC030997 RB
      MSFDVRHVY TKDISGSYR LLPQSSGRC QNIPISVEP 
      LDREAEEDC QPIMCESQS SVDEIMSLD GLDNDLKKA 
      TNKAILSEQ QKYADENLF LRYLELDPT EGPEAIPAA 
      MQRKATGNK NGRTPFTQT KKLTRAPKS GFGFSVVWT 
      HPPRIERVE KGLPAEKAG ILPGDYIIF VDKHNVVMM 
      PEIDILNLI RSYGSQLTL EIFRRNAAK NGSVPSVRR 
      LNSTGTCST TGLPISSNL VQRRPSTVC STNTASVDY 
      NRRKLHLPQ VTFSAEKAS NNQEENRTK AMYQLIHKE 
      QQYATGLQF AVTRFVSAL AERRDLITP SEHKILFQN 
      SEEILRVTE DILDNLVHE EGVHNLIRT YHAKLNEIT 
      AAYRRYCSG IKKADCVLA NKTKNSNSE FVRFLHTPS 
      IPRRRPDIT AFIHKPLEH YREVLKAFT VIQSHTKPN 
      HEDYPVINQ LVHDLQHTY REITVEAGL MEPMGEGRP 
      LLSVQDLEN RLVFTKCKP FVLNKPGRQ WIFGGDLGR 
      VEGRNVRQY WTLLFSDLL LFAKVSRDR VLFIIEDPL 
      PLAHITDMF FNVRKKDTE FRITVTPGG RLASSPTVH 
      CGPDLSRTP KKNTGKRTV ILRAPTTEL KAVWQNLLQ 
      RQIFHVNAG MDGSSFSSP LESPDAPIT SSVATLQSA 
      ESLSFRRQV RLCSGHCSE PVDNSCLPA QNRSPRHHQ 
      PSLPTQTSL TKTPDTPSA NISSQSSSE QSQAFTSHF 
      TSESSLVPK YYLPEEPQS PSAILTPST EISSDADAH 
      NSEDIDFLK FDEDPWDIT NFDYDLSSL NIDGLSTKK 
      MQKQIDALI EHKCKQLSK NNSGKGNAI HLEKWMKGH 
      LDSDIADEA ELEPLSEEW TEEMLRKRS EELQLIDAQ 
      GNVTSRNDT RRIESRCED NNLSDHDQS PSKSTTTES 
      QVTVRSSPL VPETVPVCR QCHKNCLPS VNNSVKSAR 
      SNNNTENPK KDENDNKDE DDWRPLLLM GLSAINPAA 
      SLVKLDPFS TPIPQINVA PPTPESSTK TGSTSDQKQ 
      KDGSCSCRN SRPELNNLE LSNEVDISP DDSPQTEEH 
      PYHSLSSSN LTLRRYGTV SSLERLGTD EHEDNWDER 
      YSSDEENEE RGGIDNEAF YHSSIKSWT AKAGSFVAE 
      KMSFFERLG EDYRSTGRF FERYLKTAE TTVNGDDVQ 
      EDETSGATS GEEIWGTPT SGGEMEDPL SSPNYEGKQ 
      SPYDGSISS DNGDDTELM MDELLMTPP ITGAIMRGL 
      LPRRTLEPL IEEDFSESC SPSSSGTPT EPSMSPEQG 
      NGTGDVADK CSTAVENRI ETPEMGSAV TTSEATAVP 
      KIHRSESYR KIVEAAEED YDDALGFFD RFKPTVKFI 
      NIERVPRAK SAKIFEFFN MRRERRIHQ TIPEGKQLI 
      KIFSKEGTI SDNVPTSYA EKPLSPRRL KDKQMDRRF 
      WKQLSRRRG NKMSNLPA
    CDS sequence
    • >TC030997 RA
      atgctgaag tggactgta atggctcgg cgagaggcc 
      gacaaatcc gacgccttg cgccgtcca acggcggat 
      gcttcaacc agcgaaacg actccgaag agtaataaa 
      acgaaacgt cggcactcc agcggccat cggcggtcc 
      gggggccgg cggtcgagc ttgggcctc gagatggat 
      aaagagaac cagttcacg tccactccg atcaagtcc 
      ttcgaggac catcaccaa ttcgacgct ttgcgggac 
      gtcagcaat ctaacaccg aaccgacga gtgctctcc 
      gctaaaaaa tgcaggagg agtgcgtcg ccctcctcc 
      cataggaag cataagaag aaaaagctc tgtctcaac 
      ccctcgcac aggaaagag ttcggagag ggcaagaac 
      tatttcagg cccttcgaa acggtcgac tcgcacatt 
      gagggggtg ctcgttgac aaggacctc agtggagaa 
      ttgcgggtg aaaaggcgc atccaggac acgtacgca 
      cccaccctg cccactttc accacggaa tactcgccc 
      tgtttcatg agggatgcc cactgtctt tacaacttc 
      acccccgcg aaagcgacc cccctgggg aagtatcta 
      gcactggac caagacact ccccacccg agcaaaaaa 
      ccgaaaatc gaccacgtc agtgatttt ctgcatcaa 
      attaatttc ataacgtcc ccggaagac gacaaactg 
      acttgcccc acgatggag tccaagtca accctcaca 
      ttacgcgag gagtccaaa gtctcgcct ctagtcaaa 
      aaattagta gatctaagg tttagtcaa ttaagttgc 
      gataaggac acttgtaaa aacaactcg tccctaatt 
      aacgactta tccctcgac caaatcgtc gacgccatc 
      ctcaactcc tcggccgag agcgcggcc aacaaagag 
      aacgaatcg aacgaaacg cacgaagaa aacttaata 
      aacacagaa aacgagcga cacgtcgcc acttccttt 
      gatcgctgc tcttccgac tcgggcttc aagagctcg 
      acgacggaa tactcgcac caactcgag ggcaatttc 
      cagtgcaag tgcaagacc tccaccccg aacgccgca 
      ctttgcgac aagaccatc atcaacatc aatgaaact 
      ttcaacgaa cgctgcgtg gacgtgata aacccgagg 
      aaaaggccg tcgtcgacg cccaacgac gacaataac 
      gccaaacgc gcctatttg gacaaatcc gaggaggac 
      aacgtttac tgcaccctg aaacgacaa cggtgcatc 
      cgccgccgg agaaccgac gacgagaag aaaatgagc 
      gccaagaaa tcgcgcaaa gtggacgaa cccataatc 
      ggggacgag tcgttcgac tccaatatt tcgggaaaa 
      attgccgaa gataacagc acgccgctg aatgaaact 
      tttacgctt ttgacgcca gtggataac agtcggagg 
      tttcggaga tgcctgtta ttcgaatcg cccacgtcg 
      ttgtccgaa agcgcttcg accaccaac agcagcctc 
      cgggacgtg agagggacc atggatttg aacattcgt 
      tgcgaaaac gacgaatta tacgccaac ataattcgc 
      tgcaaggat ttgtacaga cccaacggg aagcaaata 
      aacgcttat gtgaaggtt gccttaagt gatcgacta 
      ggagataat cgcaggaaa cggaacgga atattacag 
      cgtaccgcc gttcaagcc gactcctcc aaacccttc 
      ttcaaccac acttttaaa tttcctctc cagaaggaa 
      agccttcaa aagcgatta catatcgaa gtttggcat 
      cgggatcgc agctcaaga acaagcgaa tttttgggg 
      tgtatgtcc tttgatgtc agacatgtg tatacgaag 
      gatatcagc ggttcatat cgtttgctg cctcagtcg 
      tcaggacgc tgtcaaaac attccgata agcgtcgag 
      ccgttagat cgcgaagca gaggaagac tgccagcct 
      ataatgtgc gagagtcag tcgagcgtc gacgagatc 
      atgagcctt gatggtctc gacaacgac ttgaagaaa 
      gccaccaac aaggcgatc ctgagcgag cagcagaaa 
      tatgcagat gagaacttg ttcctgcgc tatctggag 
      ctggaccct acggaaggg ccggaagcc atccccgcc 
      gcgatgcag agaaaagcg acggggaac aaaaacggc 
      cggacgccc tttacgcag acgaagaag ttgacgcgt 
      gctcccaaa tccgggttc ggcttctcg gtcgtctgg 
      acgcatccg ccgcggata gaaagggtc gagaagggg 
      ctgccggcg gagaaagcg gggattttg ccgggggat 
      tacattatc ttcgtcgat aaacacaat gtcgtcatg 
      atgccagaa attgatatt ttgaattta attaggtcg 
      tatggaagt caattaact ttagaaata tttagaaga 
      aacgcggcg aaaaacggt tctgtgcca agcgtcagg 
      cgtttaaat agtaccgga acttgcagc acgacgggg 
      cttcctata agctctaat ttagtgcaa agacgtccc 
      tccactgtt tgttcaacg aatacagcc tcagtcgat 
      tacaacagg agaaaattg catttacct caagttact 
      tttagtgca gagaaagcc agtaataac caagaagaa 
      aatagaacg aaagccatg tatcaacta atacataaa 
      gaacaacaa tacgcaaca gggttacaa tttgcagtt 
      acaagattc gtatcagct ctggctgag cggcgagac 
      ttaattaca ccttccgaa cataaaatt ctttttcaa 
      aattccgaa gagattctt agagtaaca gaggatatc 
      ctcgacaat ttagttcac gaagagggt gttcataat 
      ttaattcgg acgtatcac gcaaaattg aacgaaatt 
      acagcagct tacagacgc tattgctca ggaattaaa 
      aaagctgac tgcgtatta gcaaataaa acgaaaaat 
      tccaattcg gagtttgtt aggttctta cacacgcca 
      tcgattcct agaagacgg ccagacatt actgcattt 
      attcataaa cctctcgag cattacagg gaagttttg 
      aaagctttc actgttata caaagccac acaaagcct 
      aatcatgaa gactatcct gtgataaat caacttgtg 
      catgacttg cagcacact tatagagaa attacagta 
      gaggcagga ctcatggaa ccgatgggg gaaggccga 
      cctctccta tcagtccaa gacttagaa aatcgttta 
      gttttcaca aagtgtaaa cctttcgtt ttaaataaa 
      cccggtcgc caatggata ttcggaggc gatttaggg 
      cgagtggaa ggacgaaac gtacgacaa tactggacg 
      cttctattt agtgattta ttattattt gccaaagtt 
      tcgagagac cgagtttta ttcattatt gaagaccct 
      ttaccctta gcacacata actgacatg tttttcaac 
      gttaggaaa aaagatact gaatttagg ataactgtt 
      acaccaggt ggaagactt gcgagtagt ccaactgta 
      cactgcgga cctgactta tcgagaact ccgaaaaaa 
      aatacgggt aaaaggaca gtgatttta agggcaccg 
      acgacggag ctgaaagcg gtttggcaa aatctgctc 
      cagcggcaa atcttccac gtaaacgcg ggcatggac 
      ggcagctca tttagcagc ccgttagaa tctcccgac 
      gctccaata acctcctcc gtggccacc cttcagtct 
      gcagaatcg ctctccttc cgtcggcag gtgcgtctg 
      tgtagcggt cactgcagc gaaccagta gataactcc 
      tgcctacct gcacaaaac cgctcccca cgccaccat 
      cagccctca ttgcccaca caaacatcg ctgaccaaa 
      acccccgac acaccctcg gcgaacatt tcctcgcaa 
      tcgtcctcc gagcaatct caggctttc acctcgcat 
      ttcacttcc gagagtagt ttggtacca aagtactac 
      ctacccgaa gaaccgcaa tccccttcc gctatcctc 
      actccttca acggagatc tcatccgac gctgatgca 
      cacaactcg gaagacata gacttttta aaatttgac 
      gaggatccg tgggatatc acgaatttc gattacgat 
      ctcagtagt ctgaatatc gacgggttg tcgaccaaa 
      aaaatgcaa aagcaaata gacgccctt atagagcat 
      aagtgtaaa caattgagc aaaaataat agcggaaaa 
      ggtaatgct attcattta gaaaaatgg atgaaaggt 
      catcttgat tctgatatt gctgatgaa gcagaattg 
      gaaccttta tccgaagaa tggaccgaa gaaatgctc 
      cgaaaacgg tcagaagaa ctccagtta atagacgcc 
      caagggaac gttacctct cgtaacgac acccgaaga 
      atcgaaagc agatgcgaa gacaacaac ttatctgac 
      cacgatcaa agtccgtcc aaaagtaca acaacagaa 
      agtcaagtc accgtgcgc tcaagcccc ttagtgcca 
      gaaactgtg ccagtgtgc cgacagtgc cataaaaat 
      tgccttcca tctgtaaac aatagtgtc aaaagtgct 
      cgatctaac aataacacc gaaaatcca aaaaaagac 
      gaaaatgat aataaagac gaagacgac tggagaccc 
      ctccttctg atgggctta agtgcaatc aacccagcg 
      gctagtcta gtcaaatta gatccgttt tctacacca 
      atcccacaa ataaacgtc gcgccacca acaccagaa 
      agctccacc aagaccgga agcacttcc gatcagaaa 
      cagaaagac ggttcctgc agttgtcgc aatagcagg 
      cctgaattg aataactta gaactatcc aacgaagtt 
      gatatatca ccagacgat tcacctcaa actgaagaa 
      catccatat cactcgtta agcagctca aacttaaca 
      ctacgaaga tacggtaca gtttctagt ttagaacga 
      ttaggaaca gacgaacac gaagacaac tgggatgaa 
      cgatattcg tctgatgaa gaaaacgaa gaacgtgga 
      ggtattgac aacgaagca ttttaccat tcatctatt 
      aaaagttgg actgcgaaa gctggctct tttgttgcc 
      gaaaaaatg tcgtttttt gaacgactt ggagaagat 
      tatagaagc actgggcgg ttttttgaa agatattta 
      aaaacagca gaaactaca gttaatgga gatgacgtc 
      caagaagac gaaacatct ggcgctact tctggcgaa 
      gaaatatgg ggaacaccc accagtggt ggtgaaatg 
      gaagatccc ttgagttca ccaaattat gaaggaaaa 
      caatcgcct tatgacggt tcaatatcg tccgacaat 
      ggagacgac acagaactc atgatggac gagctgcta 
      atgacccct cccatcact ggtgccatt atgagaggt 
      cttcttcct cgcaggaca ctagaaccg ttgatagaa 
      gaagacttt tcggagagt tgttctccc tcatcatcg 
      ggaacccca accgagcct tccatgtct ccggaacag 
      ggcaacgga actggcgat gttgctgac aaatgttcg 
      actgctgta gaaaaccgg atagagact ccagagatg 
      gggtcggca gttacgacg tccgaagcg actgctgtt 
      ccgaaaatt catcgatcg gaaagttac cgcaagatt 
      gtagaagcg gcggaagaa gactacgac gacgccctc 
      ggttttttc gaccggttc aagccaaca gtgaagttc 
      ataaatatt gagcgagta ccgcgagca aaaagtgca 
      aaaattttt gagtttttt aacatgcgg cgcgaacgc 
      cgaatccac caaaccatt ccggaagga aaacaatta 
      atcaaaatt ttcagtaaa gaaggcacg atttcagac 
      aatgttcct acgtcttat gcggaaaaa ccgctttcg 
      cctagacga ttaaaggat aagcagatg gatcgaagg 
      ttttggaaa cagttgagt agacgtcgt ggcaataaa 
      atgtcaaac ctgccggcc taa
    • >TC030997 RB
      atgtccttt gatgtcaga catgtgtat acgaaggat 
      atcagcggt tcatatcgt ttgctgcct cagtcgtca 
      ggacgctgt caaaacatt ccgataagc gtcgagccg 
      ttagatcgc gaagcagag gaagactgc cagcctata 
      atgtgcgag agtcagtcg agcgtcgac gagatcatg 
      agccttgat ggtctcgac aacgacttg aagaaagcc 
      accaacaag gcgatcctg agcgagcag cagaaatat 
      gcagatgag aacttgttc ctgcgctat ctggagctg 
      gaccctacg gaagggccg gaagccatc cccgccgcg 
      atgcagaga aaagcgacg gggaacaaa aacggccgg 
      acgcccttt acgcagacg aagaagttg acgcgtgct 
      cccaaatcc gggttcggc ttctcggtc gtctggacg 
      catccgccg cggatagaa agggtcgag aaggggctg 
      ccggcggag aaagcgggg attttgccg ggggattac 
      attatcttc gtcgataaa cacaatgtc gtcatgatg 
      ccagaaatt gatattttg aatttaatt aggtcgtat 
      ggaagtcaa ttaacttta gaaatattt agaagaaac 
      gcggcgaaa aacggttct gtgccaagc gtcaggcgt 
      ttaaatagt accggaact tgcagcacg acggggctt 
      cctataagc tctaattta gtgcaaaga cgtccctcc 
      actgtttgt tcaacgaat acagcctca gtcgattac 
      aacaggaga aaattgcat ttacctcaa gttactttt 
      agtgcagag aaagccagt aataaccaa gaagaaaat 
      agaacgaaa gccatgtat caactaata cataaagaa 
      caacaatac gcaacaggg ttacaattt gcagttaca 
      agattcgta tcagctctg gctgagcgg cgagactta 
      attacacct tccgaacat aaaattctt tttcaaaat 
      tccgaagag attcttaga gtaacagag gatatcctc 
      gacaattta gttcacgaa gagggtgtt cataattta 
      attcggacg tatcacgca aaattgaac gaaattaca 
      gcagcttac agacgctat tgctcagga attaaaaaa 
      gctgactgc gtattagca aataaaacg aaaaattcc 
      aattcggag tttgttagg ttcttacac acgccatcg 
      attcctaga agacggcca gacattact gcatttatt 
      cataaacct ctcgagcat tacagggaa gttttgaaa 
      gctttcact gttatacaa agccacaca aagcctaat 
      catgaagac tatcctgtg ataaatcaa cttgtgcat 
      gacttgcag cacacttat agagaaatt acagtagag 
      gcaggactc atggaaccg atgggggaa ggccgacct 
      ctcctatca gtccaagac ttagaaaat cgtttagtt 
      ttcacaaag tgtaaacct ttcgtttta aataaaccc 
      ggtcgccaa tggatattc ggaggcgat ttagggcga 
      gtggaagga cgaaacgta cgacaatac tggacgctt 
      ctatttagt gatttatta ttatttgcc aaagtttcg 
      agagaccga gttttattc attattgaa gacccttta 
      cccttagca cacataact gacatgttt ttcaacgtt 
      aggaaaaaa gatactgaa tttaggata actgttaca 
      ccaggtgga agacttgcg agtagtcca actgtacac 
      tgcggacct gacttatcg agaactccg aaaaaaaat 
      acgggtaaa aggacagtg attttaagg gcaccgacg 
      acggagctg aaagcggtt tggcaaaat ctgctccag 
      cggcaaatc ttccacgta aacgcgggc atggacggc 
      agctcattt agcagcccg ttagaatct cccgacgct 
      ccaataacc tcctccgtg gccaccctt cagtctgca 
      gaatcgctc tccttccgt cggcaggtg cgtctgtgt 
      agcggtcac tgcagcgaa ccagtagat aactcctgc 
      ctacctgca caaaaccgc tccccacgc caccatcag 
      ccctcattg cccacacaa acatcgctg accaaaacc 
      cccgacaca ccctcggcg aacatttcc tcgcaatcg 
      tcctccgag caatctcag gctttcacc tcgcatttc 
      acttccgag agtagtttg gtaccaaag tactaccta 
      cccgaagaa ccgcaatcc ccttccgct atcctcact 
      ccttcaacg gagatctca tccgacgct gatgcacac 
      aactcggaa gacatagac tttttaaaa tttgacgag 
      gatccgtgg gatatcacg aatttcgat tacgatctc 
      agtagtctg aatatcgac gggttgtcg accaaaaaa 
      atgcaaaag caaatagac gcccttata gagcataag 
      tgtaaacaa ttgagcaaa aataatagc ggaaaaggt 
      aatgctatt catttagaa aaatggatg aaaggtcat 
      cttgattct gatattgct gatgaagca gaattggaa 
      cctttatcc gaagaatgg accgaagaa atgctccga 
      aaacggtca gaagaactc cagttaata gacgcccaa 
      gggaacgtt acctctcgt aacgacacc cgaagaatc 
      gaaagcaga tgcgaagac aacaactta tctgaccac 
      gatcaaagt ccgtccaaa agtacaaca acagaaagt 
      caagtcacc gtgcgctca agcccctta gtgccagaa 
      actgtgcca gtgtgccga cagtgccat aaaaattgc 
      cttccatct gtaaacaat agtgtcaaa agtgctcga 
      tctaacaat aacaccgaa aatccaaaa aaagacgaa 
      aatgataat aaagacgaa gacgactgg agacccctc 
      cttctgatg ggcttaagt gcaatcaac ccagcggct 
      agtctagtc aaattagat ccgttttct acaccaatc 
      ccacaaata aacgtcgcg ccaccaaca ccagaaagc 
      tccaccaag accggaagc acttccgat cagaaacag 
      aaagacggt tcctgcagt tgtcgcaat agcaggcct 
      gaattgaat aacttagaa ctatccaac gaagttgat 
      atatcacca gacgattca cctcaaact gaagaacat 
      ccatatcac tcgttaagc agctcaaac ttaacacta 
      cgaagatac ggtacagtt tctagttta gaacgatta 
      ggaacagac gaacacgaa gacaactgg gatgaacga 
      tattcgtct gatgaagaa aacgaagaa cgtggaggt 
      attgacaac gaagcattt taccattca tctattaaa 
      agttggact gcgaaagct ggctctttt gttgccgaa 
      aaaatgtcg ttttttgaa cgacttgga gaagattat 
      agaagcact gggcggttt tttgaaaga tatttaaaa 
      acagcagaa actacagtt aatggagat gacgtccaa 
      gaagacgaa acatctggc gctacttct ggcgaagaa 
      atatgggga acacccacc agtggtggt gaaatggaa 
      gatcccttg agttcacca aattatgaa ggaaaacaa 
      tcgccttat gacggttca atatcgtcc gacaatgga 
      gacgacaca gaactcatg atggacgag ctgctaatg 
      acccctccc atcactggt gccattatg agaggtctt 
      cttcctcgc aggacacta gaaccgttg atagaagaa 
      gacttttcg gagagttgt tctccctca tcatcggga 
      accccaacc gagccttcc atgtctccg gaacagggc 
      aacggaact ggcgatgtt gctgacaaa tgttcgact 
      gctgtagaa aaccggata gagactcca gagatgggg 
      tcggcagtt acgacgtcc gaagcgact gctgttccg 
      aaaattcat cgatcggaa agttaccgc aagattgta 
      gaagcggcg gaagaagac tacgacgac gccctcggt 
      tttttcgac cggttcaag ccaacagtg aagttcata 
      aatattgag cgagtaccg cgagcaaaa agtgcaaaa 
      atttttgag ttttttaac atgcggcgc gaacgccga 
      atccaccaa accattccg gaaggaaaa caattaatc 
      aaaattttc agtaaagaa ggcacgatt tcagacaat 
      gttcctacg tcttatgcg gaaaaaccg ctttcgcct 
      agacgatta aaggataag cagatggat cgaaggttt 
      tggaaacag ttgagtaga cgtcgtggc aataaaatg 
      tcaaacctg ccggcctaa
    mRNA sequence
    • >TC030997 RA
      agattctga tgcatgcgc gaggattta cgccggtat 
      ttaaatgaa ataacgcgg gagaaatgt tttgcgatt 
      gattttgtg ctttggtga agtgatttt tgcgatttt 
      ggattatta accgacccg aaccatgct gaagtggac 
      tgtaatggc tcggcgaga ggccgacaa atccgacgc 
      cttgcgccg tccaacggc ggatgcttc aaccagcga 
      aacgactcc gaagagtaa taaaacgaa acgtcggca 
      ctccagcgg ccatcggcg gtccggggg ccggcggtc 
      gagcttggg cctcgagat ggataaaga gaaccagtt 
      cacgtccac tccgatcaa gtccttcga ggaccatca 
      ccaattcga cgctttgcg ggacgtcag caatctaac 
      accgaaccg acgagtgct ctccgctaa aaaatgcag 
      gaggagtgc gtcgccctc ctcccatag gaagcataa 
      gaagaaaaa gctctgtct caacccctc gcacaggaa 
      agagttcgg agagggcaa gaactattt caggccctt 
      cgaaacggt cgactcgca cattgaggg ggtgctcgt 
      tgacaagga cctcagtgg agaattgcg ggtgaaaag 
      gcgcatcca ggacacgta cgcacccac cctgcccac 
      tttcaccac ggaatactc gccctgttt catgaggga 
      tgcccactg tctttacaa cttcacccc cgcgaaagc 
      gacccccct ggggaagta tctagcact ggaccaaga 
      cactcccca cccgagcaa aaaaccgaa aatcgacca 
      cgtcagtga ttttctgca tcaaattaa tttcataac 
      gtccccgga agacgacaa actgacttg ccccacgat 
      ggagtccaa gtcaaccct cacattacg cgaggagtc 
      caaagtctc gcctctagt caaaaaatt agtagatct 
      aaggtttag tcaattaag ttgcgataa ggacacttg 
      taaaaacaa ctcgtccct aattaacga cttatccct 
      cgaccaaat cgtcgacgc catcctcaa ctcctcggc 
      cgagagcgc ggccaacaa agagaacga atcgaacga 
      aacgcacga agaaaactt aataaacac agaaaacga 
      gcgacacgt cgccacttc ctttgatcg ctgctcttc 
      cgactcggg cttcaagag ctcgacgac ggaatactc 
      gcaccaact cgagggcaa tttccagtg caagtgcaa 
      gacctccac cccgaacgc cgcactttg cgacaagac 
      catcatcaa catcaatga aactttcaa cgaacgctg 
      cgtggacgt gataaaccc gaggaaaag gccgtcgtc 
      gacgcccaa cgacgacaa taacgccaa acgcgccta 
      tttggacaa atccgagga ggacaacgt ttactgcac 
      cctgaaacg acaacggtg catccgccg ccggagaac 
      cgacgacga gaagaaaat gagcgccaa gaaatcgcg 
      caaagtgga cgaacccat aatcgggga cgagtcgtt 
      cgactccaa tatttcggg aaaaattgc cgaagataa 
      cagcacgcc gctgaatga aacttttac gcttttgac 
      gccagtgga taacagtcg gaggtttcg gagatgcct 
      gttattcga atcgcccac gtcgttgtc cgaaagcgc 
      ttcgaccac caacagcag cctccggga cgtgagagg 
      gaccatgga tttgaacat tcgttgcga aaacgacga 
      attatacgc caacataat tcgctgcaa ggatttgta 
      cagacccaa cgggaagca aataaacgc ttatgtgaa 
      ggttgcctt aagtgatcg actaggaga taatcgcag 
      gaaacggaa cggaatatt acagcgtac cgccgttca 
      agccgactc ctccaaacc cttcttcaa ccacacttt 
      taaatttcc tctccagaa ggaaagcct tcaaaagcg 
      attacatat cgaagtttg gcatcggga tcgcagctc 
      aagaacaag cgaattttt ggggtgtat gtcctttga 
      tgtcagaca tgtgtatac gaaggatat cagcggttc 
      atatcgttt gctgcctca gtcgtcagg acgctgtca 
      aaacattcc gataagcgt cgagccgtt agatcgcga 
      agcagagga agactgcca gcctataat gtgcgagag 
      tcagtcgag cgtcgacga gatcatgag ccttgatgg 
      tctcgacaa cgacttgaa gaaagccac caacaaggc 
      gatcctgag cgagcagca gaaatatgc agatgagaa 
      cttgttcct gcgctatct ggagctgga ccctacgga 
      agggccgga agccatccc cgccgcgat gcagagaaa 
      agcgacggg gaacaaaaa cggccggac gccctttac 
      gcagacgaa gaagttgac gcgtgctcc caaatccgg 
      gttcggctt ctcggtcgt ctggacgca tccgccgcg 
      gatagaaag ggtcgagaa ggggctgcc ggcggagaa 
      agcggggat tttgccggg ggattacat tatcttcgt 
      cgataaaca caatgtcgt catgatgcc agaaattga 
      tattttgaa tttaattag gtcgtatgg aagtcaatt 
      aactttaga aatatttag aagaaacgc ggcgaaaaa 
      cggttctgt gccaagcgt caggcgttt aaatagtac 
      cggaacttg cagcacgac ggggcttcc tataagctc 
      taatttagt gcaaagacg tccctccac tgtttgttc 
      aacgaatac agcctcagt cgattacaa caggagaaa 
      attgcattt acctcaagt tacttttag tgcagagaa 
      agccagtaa taaccaaga agaaaatag aacgaaagc 
      catgtatca actaataca taaagaaca acaatacgc 
      aacagggtt acaatttgc agttacaag attcgtatc 
      agctctggc tgagcggcg agacttaat tacaccttc 
      cgaacataa aattctttt tcaaaattc cgaagagat 
      tcttagagt aacagagga tatcctcga caatttagt 
      tcacgaaga gggtgttca taatttaat tcggacgta 
      tcacgcaaa attgaacga aattacagc agcttacag 
      acgctattg ctcaggaat taaaaaagc tgactgcgt 
      attagcaaa taaaacgaa aaattccaa ttcggagtt 
      tgttaggtt cttacacac gccatcgat tcctagaag 
      acggccaga cattactgc atttattca taaacctct 
      cgagcatta cagggaagt tttgaaagc tttcactgt 
      tatacaaag ccacacaaa gcctaatca tgaagacta 
      tcctgtgat aaatcaact tgtgcatga cttgcagca 
      cacttatag agaaattac agtagaggc aggactcat 
      ggaaccgat gggggaagg ccgacctct cctatcagt 
      ccaagactt agaaaatcg tttagtttt cacaaagtg 
      taaaccttt cgttttaaa taaacccgg tcgccaatg 
      gatattcgg aggcgattt agggcgagt ggaaggacg 
      aaacgtacg acaatactg gacgcttct atttagtga 
      tttattatt atttgccaa agtttcgag agaccgagt 
      tttattcat tattgaaga ccctttacc cttagcaca 
      cataactga catgttttt caacgttag gaaaaaaga 
      tactgaatt taggataac tgttacacc aggtggaag 
      acttgcgag tagtccaac tgtacactg cggacctga 
      cttatcgag aactccgaa aaaaaatac gggtaaaag 
      gacagtgat tttaagggc accgacgac ggagctgaa 
      agcggtttg gcaaaatct gctccagcg gcaaatctt 
      ccacgtaaa cgcgggcat ggacggcag ctcatttag 
      cagcccgtt agaatctcc cgacgctcc aataacctc 
      ctccgtggc cacccttca gtctgcaga atcgctctc 
      cttccgtcg gcaggtgcg tctgtgtag cggtcactg 
      cagcgaacc agtagataa ctcctgcct acctgcaca 
      aaaccgctc cccacgcca ccatcagcc ctcattgcc 
      cacacaaac atcgctgac caaaacccc cgacacacc 
      ctcggcgaa catttcctc gcaatcgtc ctccgagca 
      atctcaggc tttcacctc gcatttcac ttccgagag 
      tagtttggt accaaagta ctacctacc cgaagaacc 
      gcaatcccc ttccgctat cctcactcc ttcaacgga 
      gatctcatc cgacgctga tgcacacaa ctcggaaga 
      catagactt tttaaaatt tgacgagga tccgtggga 
      tatcacgaa tttcgatta cgatctcag tagtctgaa 
      tatcgacgg gttgtcgac caaaaaaat gcaaaagca 
      aatagacgc ccttataga gcataagtg taaacaatt 
      gagcaaaaa taatagcgg aaaaggtaa tgctattca 
      tttagaaaa atggatgaa aggtcatct tgattctga 
      tattgctga tgaagcaga attggaacc tttatccga 
      agaatggac cgaagaaat gctccgaaa acggtcaga 
      agaactcca gttaataga cgcccaagg gaacgttac 
      ctctcgtaa cgacacccg aagaatcga aagcagatg 
      cgaagacaa caacttatc tgaccacga tcaaagtcc 
      gtccaaaag tacaacaac agaaagtca agtcaccgt 
      gcgctcaag ccccttagt gccagaaac tgtgccagt 
      gtgccgaca gtgccataa aaattgcct tccatctgt 
      aaacaatag tgtcaaaag tgctcgatc taacaataa 
      caccgaaaa tccaaaaaa agacgaaaa tgataataa 
      agacgaaga cgactggag acccctcct tctgatggg 
      cttaagtgc aatcaaccc agcggctag tctagtcaa 
      attagatcc gttttctac accaatccc acaaataaa 
      cgtcgcgcc accaacacc agaaagctc caccaagac 
      cggaagcac ttccgatca gaaacagaa agacggttc 
      ctgcagttg tcgcaatag caggcctga attgaataa 
      cttagaact atccaacga agttgatat atcaccaga 
      cgattcacc tcaaactga agaacatcc atatcactc 
      gttaagcag ctcaaactt aacactacg aagatacgg 
      tacagtttc tagtttaga acgattagg aacagacga 
      acacgaaga caactggga tgaacgata ttcgtctga 
      tgaagaaaa cgaagaacg tggaggtat tgacaacga 
      agcatttta ccattcatc tattaaaag ttggactgc 
      gaaagctgg ctcttttgt tgccgaaaa aatgtcgtt 
      ttttgaacg acttggaga agattatag aagcactgg 
      gcggttttt tgaaagata tttaaaaac agcagaaac 
      tacagttaa tggagatga cgtccaaga agacgaaac 
      atctggcgc tacttctgg cgaagaaat atggggaac 
      acccaccag tggtggtga aatggaaga tcccttgag 
      ttcaccaaa ttatgaagg aaaacaatc gccttatga 
      cggttcaat atcgtccga caatggaga cgacacaga 
      actcatgat ggacgagct gctaatgac ccctcccat 
      cactggtgc cattatgag aggtcttct tcctcgcag 
      gacactaga accgttgat agaagaaga cttttcgga 
      gagttgttc tccctcatc atcgggaac cccaaccga 
      gccttccat gtctccgga acagggcaa cggaactgg 
      cgatgttgc tgacaaatg ttcgactgc tgtagaaaa 
      ccggataga gactccaga gatggggtc ggcagttac 
      gacgtccga agcgactgc tgttccgaa aattcatcg 
      atcggaaag ttaccgcaa gattgtaga agcggcgga 
      agaagacta cgacgacgc cctcggttt tttcgaccg 
      gttcaagcc aacagtgaa gttcataaa tattgagcg 
      agtaccgcg agcaaaaag tgcaaaaat ttttgagtt 
      ttttaacat gcggcgcga acgccgaat ccaccaaac 
      cattccgga aggaaaaca attaatcaa aattttcag 
      taaagaagg cacgatttc agacaatgt tcctacgtc 
      ttatgcgga aaaaccgct ttcgcctag acgattaaa 
      ggataagca gatggatcg aaggttttg gaaacagtt 
      gagtagacg tcgtggcaa taaaatgtc aaacctgcc 
      ggcctaatt atcgcaata taatagttt ttcaaaa
    • >TC030997 RB
      ggttcgcag tgaattgtg ataatgcgt ggcgcttag 
      gtttagtga attgtgaac ggttgcggt tgtgactat 
      cccgagggg gagccgaat ttgcgccac tcaacagat 
      tgcaggata atcgggaca cggtttatc gcaagtgct 
      cgaaaacta ttaaaatcg ggataaggg agccgctgt 
      tttcgtcat attttcccg caggaacgc atcaatctt 
      actccttga caaattgat ggctctccc taaacaata 
      tcaaatgag gctctagtc ctttattat tttttaggt 
      ttcttttta tacagtaac caagtgcca tgtactgta 
      caatttgtg actaaatgt atcgtgatt gtttgtgac 
      tcagtgtcc tttaattat ctcaaatca aaaaatgag 
      ctttcccga aaattgtgt gaaagatga tcaagtttc 
      ggggagttt aaccatcac attacgtca cctcatcta 
      gatggaaaa aaacaagcg aatttttgg ggtgtatgt 
      cctttgatg tcagacatg tgtatacga aggatatca 
      gcggttcat atcgtttgc tgcctcagt cgtcaggac 
      gctgtcaaa acattccga taagcgtcg agccgttag 
      atcgcgaag cagaggaag actgccagc ctataatgt 
      gcgagagtc agtcgagcg tcgacgaga tcatgagcc 
      ttgatggtc tcgacaacg acttgaaga aagccacca 
      acaaggcga tcctgagcg agcagcaga aatatgcag 
      atgagaact tgttcctgc gctatctgg agctggacc 
      ctacggaag ggccggaag ccatccccg ccgcgatgc 
      agagaaaag cgacgggga acaaaaacg gccggacgc 
      cctttacgc agacgaaga agttgacgc gtgctccca 
      aatccgggt tcggcttct cggtcgtct ggacgcatc 
      cgccgcgga tagaaaggg tcgagaagg ggctgccgg 
      cggagaaag cggggattt tgccggggg attacatta 
      tcttcgtcg ataaacaca atgtcgtca tgatgccag 
      aaattgata ttttgaatt taattaggt cgtatggaa 
      gtcaattaa ctttagaaa tatttagaa gaaacgcgg 
      cgaaaaacg gttctgtgc caagcgtca ggcgtttaa 
      atagtaccg gaacttgca gcacgacgg ggcttccta 
      taagctcta atttagtgc aaagacgtc cctccactg 
      tttgttcaa cgaatacag cctcagtcg attacaaca 
      ggagaaaat tgcatttac ctcaagtta cttttagtg 
      cagagaaag ccagtaata accaagaag aaaatagaa 
      cgaaagcca tgtatcaac taatacata aagaacaac 
      aatacgcaa cagggttac aatttgcag ttacaagat 
      tcgtatcag ctctggctg agcggcgag acttaatta 
      caccttccg aacataaaa ttctttttc aaaattccg 
      aagagattc ttagagtaa cagaggata tcctcgaca 
      atttagttc acgaagagg gtgttcata atttaattc 
      ggacgtatc acgcaaaat tgaacgaaa ttacagcag 
      cttacagac gctattgct caggaatta aaaaagctg 
      actgcgtat tagcaaata aaacgaaaa attccaatt 
      cggagtttg ttaggttct tacacacgc catcgattc 
      ctagaagac ggccagaca ttactgcat ttattcata 
      aacctctcg agcattaca gggaagttt tgaaagctt 
      tcactgtta tacaaagcc acacaaagc ctaatcatg 
      aagactatc ctgtgataa atcaacttg tgcatgact 
      tgcagcaca cttatagag aaattacag tagaggcag 
      gactcatgg aaccgatgg gggaaggcc gacctctcc 
      tatcagtcc aagacttag aaaatcgtt tagttttca 
      caaagtgta aacctttcg ttttaaata aacccggtc 
      gccaatgga tattcggag gcgatttag ggcgagtgg 
      aaggacgaa acgtacgac aatactgga cgcttctat 
      ttagtgatt tattattat ttgccaaag tttcgagag 
      accgagttt tattcatta ttgaagacc ctttaccct 
      tagcacaca taactgaca tgtttttca acgttagga 
      aaaaagata ctgaattta ggataactg ttacaccag 
      gtggaagac ttgcgagta gtccaactg tacactgcg 
      gacctgact tatcgagaa ctccgaaaa aaaatacgg 
      gtaaaagga cagtgattt taagggcac cgacgacgg 
      agctgaaag cggtttggc aaaatctgc tccagcggc 
      aaatcttcc acgtaaacg cgggcatgg acggcagct 
      catttagca gcccgttag aatctcccg acgctccaa 
      taacctcct ccgtggcca cccttcagt ctgcagaat 
      cgctctcct tccgtcggc aggtgcgtc tgtgtagcg 
      gtcactgca gcgaaccag tagataact cctgcctac 
      ctgcacaaa accgctccc cacgccacc atcagccct 
      cattgccca cacaaacat cgctgacca aaacccccg 
      acacaccct cggcgaaca tttcctcgc aatcgtcct 
      ccgagcaat ctcaggctt tcacctcgc atttcactt 
      ccgagagta gtttggtac caaagtact acctacccg 
      aagaaccgc aatcccctt ccgctatcc tcactcctt 
      caacggaga tctcatccg acgctgatg cacacaact 
      cggaagaca tagactttt taaaatttg acgaggatc 
      cgtgggata tcacgaatt tcgattacg atctcagta 
      gtctgaata tcgacgggt tgtcgacca aaaaaatgc 
      aaaagcaaa tagacgccc ttatagagc ataagtgta 
      aacaattga gcaaaaata atagcggaa aaggtaatg 
      ctattcatt tagaaaaat ggatgaaag gtcatcttg 
      attctgata ttgctgatg aagcagaat tggaacctt 
      tatccgaag aatggaccg aagaaatgc tccgaaaac 
      ggtcagaag aactccagt taatagacg cccaaggga 
      acgttacct ctcgtaacg acacccgaa gaatcgaaa 
      gcagatgcg aagacaaca acttatctg accacgatc 
      aaagtccgt ccaaaagta caacaacag aaagtcaag 
      tcaccgtgc gctcaagcc ccttagtgc cagaaactg 
      tgccagtgt gccgacagt gccataaaa attgccttc 
      catctgtaa acaatagtg tcaaaagtg ctcgatcta 
      acaataaca ccgaaaatc caaaaaaag acgaaaatg 
      ataataaag acgaagacg actggagac ccctccttc 
      tgatgggct taagtgcaa tcaacccag cggctagtc 
      tagtcaaat tagatccgt tttctacac caatcccac 
      aaataaacg tcgcgccac caacaccag aaagctcca 
      ccaagaccg gaagcactt ccgatcaga aacagaaag 
      acggttcct gcagttgtc gcaatagca ggcctgaat 
      tgaataact tagaactat ccaacgaag ttgatatat 
      caccagacg attcacctc aaactgaag aacatccat 
      atcactcgt taagcagct caaacttaa cactacgaa 
      gatacggta cagtttcta gtttagaac gattaggaa 
      cagacgaac acgaagaca actgggatg aacgatatt 
      cgtctgatg aagaaaacg aagaacgtg gaggtattg 
      acaacgaag cattttacc attcatcta ttaaaagtt 
      ggactgcga aagctggct cttttgttg ccgaaaaaa 
      tgtcgtttt ttgaacgac ttggagaag attatagaa 
      gcactgggc ggttttttg aaagatatt taaaaacag 
      cagaaacta cagttaatg gagatgacg tccaagaag 
      acgaaacat ctggcgcta cttctggcg aagaaatat 
      ggggaacac ccaccagtg gtggtgaaa tggaagatc 
      ccttgagtt caccaaatt atgaaggaa aacaatcgc 
      cttatgacg gttcaatat cgtccgaca atggagacg 
      acacagaac tcatgatgg acgagctgc taatgaccc 
      ctcccatca ctggtgcca ttatgagag gtcttcttc 
      ctcgcagga cactagaac cgttgatag aagaagact 
      tttcggaga gttgttctc cctcatcat cgggaaccc 
      caaccgagc cttccatgt ctccggaac agggcaacg 
      gaactggcg atgttgctg acaaatgtt cgactgctg 
      tagaaaacc ggatagaga ctccagaga tggggtcgg 
      cagttacga cgtccgaag cgactgctg ttccgaaaa 
      ttcatcgat cggaaagtt accgcaaga ttgtagaag 
      cggcggaag aagactacg acgacgccc tcggttttt 
      tcgaccggt tcaagccaa cagtgaagt tcataaata 
      ttgagcgag taccgcgag caaaaagtg caaaaattt 
      ttgagtttt ttaacatgc ggcgcgaac gccgaatcc 
      accaaacca ttccggaag gaaaacaat taatcaaaa 
      ttttcagta aagaaggca cgatttcag acaatgttc 
      ctacgtctt atgcggaaa aaccgcttt cgcctagac 
      gattaaagg ataagcaga tggatcgaa ggttttgga 
      aacagttga gtagacgtc gtggcaata aaatgtcaa 
      acctgccgg cctaattat cgcaatata atagttttt 
      caaaa
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