| General information | |||
| TC030997
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10163176..10181980 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC030997 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03125 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
| Half of the larvae on the slide are stage 2 larvae. |
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC030997 RA
MLKWTVMAR READKSDAL RRPTADAST SETTPKSNK TKRRHSSGH RRSGGRRSS LGLEMDKEN QFTSTPIKS FEDHHQFDA LRDVSNLTP NRRVLSAKK CRRSASPSS HRKHKKKKL CLNPSHRKE FGEGKNYFR PFETVDSHI EGVLVDKDL SGELRVKRR IQDTYAPTL PTFTTEYSP CFMRDAHCL YNFTPAKAT PLGKYLALD QDTPHPSKK PKIDHVSDF LHQINFITS PEDDKLTCP TMESKSTLT LREESKVSP LVKKLVDLR FSQLSCDKD TCKNNSSLI NDLSLDQIV DAILNSSAE SAANKENES NETHEENLI NTENERHVA TSFDRCSSD SGFKSSTTE YSHQLEGNF QCKCKTSTP NAALCDKTI ININETFNE RCVDVINPR KRPSSTPND DNNAKRAYL DKSEEDNVY CTLKRQRCI RRRRTDDEK KMSAKKSRK VDEPIIGDE SFDSNISGK IAEDNSTPL NETFTLLTP VDNSRRFRR CLLFESPTS LSESASTTN SSLRDVRGT MDLNIRCEN DELYANIIR CKDLYRPNG KQINAYVKV ALSDRLGDN RRKRNGILQ RTAVQADSS KPFFNHTFK FPLQKESLQ KRLHIEVWH RDRSSRTSE FLGCMSFDV RHVYTKDIS GSYRLLPQS SGRCQNIPI SVEPLDREA EEDCQPIMC ESQSSVDEI MSLDGLDND LKKATNKAI LSEQQKYAD ENLFLRYLE LDPTEGPEA IPAAMQRKA TGNKNGRTP FTQTKKLTR APKSGFGFS VVWTHPPRI ERVEKGLPA EKAGILPGD YIIFVDKHN VVMMPEIDI LNLIRSYGS QLTLEIFRR NAAKNGSVP SVRRLNSTG TCSTTGLPI SSNLVQRRP STVCSTNTA SVDYNRRKL HLPQVTFSA EKASNNQEE NRTKAMYQL IHKEQQYAT GLQFAVTRF VSALAERRD LITPSEHKI LFQNSEEIL RVTEDILDN LVHEEGVHN LIRTYHAKL NEITAAYRR YCSGIKKAD CVLANKTKN SNSEFVRFL HTPSIPRRR PDITAFIHK PLEHYREVL KAFTVIQSH TKPNHEDYP VINQLVHDL QHTYREITV EAGLMEPMG EGRPLLSVQ DLENRLVFT KCKPFVLNK PGRQWIFGG DLGRVEGRN VRQYWTLLF SDLLLFAKV SRDRVLFII EDPLPLAHI TDMFFNVRK KDTEFRITV TPGGRLASS PTVHCGPDL SRTPKKNTG KRTVILRAP TTELKAVWQ NLLQRQIFH VNAGMDGSS FSSPLESPD APITSSVAT LQSAESLSF RRQVRLCSG HCSEPVDNS CLPAQNRSP RHHQPSLPT QTSLTKTPD TPSANISSQ SSSEQSQAF TSHFTSESS LVPKYYLPE EPQSPSAIL TPSTEISSD ADAHNSEDI DFLKFDEDP WDITNFDYD LSSLNIDGL STKKMQKQI DALIEHKCK QLSKNNSGK GNAIHLEKW MKGHLDSDI ADEAELEPL SEEWTEEML RKRSEELQL IDAQGNVTS RNDTRRIES RCEDNNLSD HDQSPSKST TTESQVTVR SSPLVPETV PVCRQCHKN CLPSVNNSV KSARSNNNT ENPKKDEND NKDEDDWRP LLLMGLSAI NPAASLVKL DPFSTPIPQ INVAPPTPE SSTKTGSTS DQKQKDGSC SCRNSRPEL NNLELSNEV DISPDDSPQ TEEHPYHSL SSSNLTLRR YGTVSSLER LGTDEHEDN WDERYSSDE ENEERGGID NEAFYHSSI KSWTAKAGS FVAEKMSFF ERLGEDYRS TGRFFERYL KTAETTVNG DDVQEDETS GATSGEEIW GTPTSGGEM EDPLSSPNY EGKQSPYDG SISSDNGDD TELMMDELL MTPPITGAI MRGLLPRRT LEPLIEEDF SESCSPSSS GTPTEPSMS PEQGNGTGD VADKCSTAV ENRIETPEM GSAVTTSEA TAVPKIHRS ESYRKIVEA AEEDYDDAL GFFDRFKPT VKFINIERV PRAKSAKIF EFFNMRRER RIHQTIPEG KQLIKIFSK EGTISDNVP TSYAEKPLS PRRLKDKQM DRRFWKQLS RRRGNKMSN LPA
- >TC030997 RB
MSFDVRHVY TKDISGSYR LLPQSSGRC QNIPISVEP LDREAEEDC QPIMCESQS SVDEIMSLD GLDNDLKKA TNKAILSEQ QKYADENLF LRYLELDPT EGPEAIPAA MQRKATGNK NGRTPFTQT KKLTRAPKS GFGFSVVWT HPPRIERVE KGLPAEKAG ILPGDYIIF VDKHNVVMM PEIDILNLI RSYGSQLTL EIFRRNAAK NGSVPSVRR LNSTGTCST TGLPISSNL VQRRPSTVC STNTASVDY NRRKLHLPQ VTFSAEKAS NNQEENRTK AMYQLIHKE QQYATGLQF AVTRFVSAL AERRDLITP SEHKILFQN SEEILRVTE DILDNLVHE EGVHNLIRT YHAKLNEIT AAYRRYCSG IKKADCVLA NKTKNSNSE FVRFLHTPS IPRRRPDIT AFIHKPLEH YREVLKAFT VIQSHTKPN HEDYPVINQ LVHDLQHTY REITVEAGL MEPMGEGRP LLSVQDLEN RLVFTKCKP FVLNKPGRQ WIFGGDLGR VEGRNVRQY WTLLFSDLL LFAKVSRDR VLFIIEDPL PLAHITDMF FNVRKKDTE FRITVTPGG RLASSPTVH CGPDLSRTP KKNTGKRTV ILRAPTTEL KAVWQNLLQ RQIFHVNAG MDGSSFSSP LESPDAPIT SSVATLQSA ESLSFRRQV RLCSGHCSE PVDNSCLPA QNRSPRHHQ PSLPTQTSL TKTPDTPSA NISSQSSSE QSQAFTSHF TSESSLVPK YYLPEEPQS PSAILTPST EISSDADAH NSEDIDFLK FDEDPWDIT NFDYDLSSL NIDGLSTKK MQKQIDALI EHKCKQLSK NNSGKGNAI HLEKWMKGH LDSDIADEA ELEPLSEEW TEEMLRKRS EELQLIDAQ GNVTSRNDT RRIESRCED NNLSDHDQS PSKSTTTES QVTVRSSPL VPETVPVCR QCHKNCLPS VNNSVKSAR SNNNTENPK KDENDNKDE DDWRPLLLM GLSAINPAA SLVKLDPFS TPIPQINVA PPTPESSTK TGSTSDQKQ KDGSCSCRN SRPELNNLE LSNEVDISP DDSPQTEEH PYHSLSSSN LTLRRYGTV SSLERLGTD EHEDNWDER YSSDEENEE RGGIDNEAF YHSSIKSWT AKAGSFVAE KMSFFERLG EDYRSTGRF FERYLKTAE TTVNGDDVQ EDETSGATS GEEIWGTPT SGGEMEDPL SSPNYEGKQ SPYDGSISS DNGDDTELM MDELLMTPP ITGAIMRGL LPRRTLEPL IEEDFSESC SPSSSGTPT EPSMSPEQG NGTGDVADK CSTAVENRI ETPEMGSAV TTSEATAVP KIHRSESYR KIVEAAEED YDDALGFFD RFKPTVKFI NIERVPRAK SAKIFEFFN MRRERRIHQ TIPEGKQLI KIFSKEGTI SDNVPTSYA EKPLSPRRL KDKQMDRRF WKQLSRRRG NKMSNLPA
- >TC030997 RA
atgctgaag tggactgta atggctcgg cgagaggcc gacaaatcc gacgccttg cgccgtcca acggcggat gcttcaacc agcgaaacg actccgaag agtaataaa acgaaacgt cggcactcc agcggccat cggcggtcc gggggccgg cggtcgagc ttgggcctc gagatggat aaagagaac cagttcacg tccactccg atcaagtcc ttcgaggac catcaccaa ttcgacgct ttgcgggac gtcagcaat ctaacaccg aaccgacga gtgctctcc gctaaaaaa tgcaggagg agtgcgtcg ccctcctcc cataggaag cataagaag aaaaagctc tgtctcaac ccctcgcac aggaaagag ttcggagag ggcaagaac tatttcagg cccttcgaa acggtcgac tcgcacatt gagggggtg ctcgttgac aaggacctc agtggagaa ttgcgggtg aaaaggcgc atccaggac acgtacgca cccaccctg cccactttc accacggaa tactcgccc tgtttcatg agggatgcc cactgtctt tacaacttc acccccgcg aaagcgacc cccctgggg aagtatcta gcactggac caagacact ccccacccg agcaaaaaa ccgaaaatc gaccacgtc agtgatttt ctgcatcaa attaatttc ataacgtcc ccggaagac gacaaactg acttgcccc acgatggag tccaagtca accctcaca ttacgcgag gagtccaaa gtctcgcct ctagtcaaa aaattagta gatctaagg tttagtcaa ttaagttgc gataaggac acttgtaaa aacaactcg tccctaatt aacgactta tccctcgac caaatcgtc gacgccatc ctcaactcc tcggccgag agcgcggcc aacaaagag aacgaatcg aacgaaacg cacgaagaa aacttaata aacacagaa aacgagcga cacgtcgcc acttccttt gatcgctgc tcttccgac tcgggcttc aagagctcg acgacggaa tactcgcac caactcgag ggcaatttc cagtgcaag tgcaagacc tccaccccg aacgccgca ctttgcgac aagaccatc atcaacatc aatgaaact ttcaacgaa cgctgcgtg gacgtgata aacccgagg aaaaggccg tcgtcgacg cccaacgac gacaataac gccaaacgc gcctatttg gacaaatcc gaggaggac aacgtttac tgcaccctg aaacgacaa cggtgcatc cgccgccgg agaaccgac gacgagaag aaaatgagc gccaagaaa tcgcgcaaa gtggacgaa cccataatc ggggacgag tcgttcgac tccaatatt tcgggaaaa attgccgaa gataacagc acgccgctg aatgaaact tttacgctt ttgacgcca gtggataac agtcggagg tttcggaga tgcctgtta ttcgaatcg cccacgtcg ttgtccgaa agcgcttcg accaccaac agcagcctc cgggacgtg agagggacc atggatttg aacattcgt tgcgaaaac gacgaatta tacgccaac ataattcgc tgcaaggat ttgtacaga cccaacggg aagcaaata aacgcttat gtgaaggtt gccttaagt gatcgacta ggagataat cgcaggaaa cggaacgga atattacag cgtaccgcc gttcaagcc gactcctcc aaacccttc ttcaaccac acttttaaa tttcctctc cagaaggaa agccttcaa aagcgatta catatcgaa gtttggcat cgggatcgc agctcaaga acaagcgaa tttttgggg tgtatgtcc tttgatgtc agacatgtg tatacgaag gatatcagc ggttcatat cgtttgctg cctcagtcg tcaggacgc tgtcaaaac attccgata agcgtcgag ccgttagat cgcgaagca gaggaagac tgccagcct ataatgtgc gagagtcag tcgagcgtc gacgagatc atgagcctt gatggtctc gacaacgac ttgaagaaa gccaccaac aaggcgatc ctgagcgag cagcagaaa tatgcagat gagaacttg ttcctgcgc tatctggag ctggaccct acggaaggg ccggaagcc atccccgcc gcgatgcag agaaaagcg acggggaac aaaaacggc cggacgccc tttacgcag acgaagaag ttgacgcgt gctcccaaa tccgggttc ggcttctcg gtcgtctgg acgcatccg ccgcggata gaaagggtc gagaagggg ctgccggcg gagaaagcg gggattttg ccgggggat tacattatc ttcgtcgat aaacacaat gtcgtcatg atgccagaa attgatatt ttgaattta attaggtcg tatggaagt caattaact ttagaaata tttagaaga aacgcggcg aaaaacggt tctgtgcca agcgtcagg cgtttaaat agtaccgga acttgcagc acgacgggg cttcctata agctctaat ttagtgcaa agacgtccc tccactgtt tgttcaacg aatacagcc tcagtcgat tacaacagg agaaaattg catttacct caagttact tttagtgca gagaaagcc agtaataac caagaagaa aatagaacg aaagccatg tatcaacta atacataaa gaacaacaa tacgcaaca gggttacaa tttgcagtt acaagattc gtatcagct ctggctgag cggcgagac ttaattaca ccttccgaa cataaaatt ctttttcaa aattccgaa gagattctt agagtaaca gaggatatc ctcgacaat ttagttcac gaagagggt gttcataat ttaattcgg acgtatcac gcaaaattg aacgaaatt acagcagct tacagacgc tattgctca ggaattaaa aaagctgac tgcgtatta gcaaataaa acgaaaaat tccaattcg gagtttgtt aggttctta cacacgcca tcgattcct agaagacgg ccagacatt actgcattt attcataaa cctctcgag cattacagg gaagttttg aaagctttc actgttata caaagccac acaaagcct aatcatgaa gactatcct gtgataaat caacttgtg catgacttg cagcacact tatagagaa attacagta gaggcagga ctcatggaa ccgatgggg gaaggccga cctctccta tcagtccaa gacttagaa aatcgttta gttttcaca aagtgtaaa cctttcgtt ttaaataaa cccggtcgc caatggata ttcggaggc gatttaggg cgagtggaa ggacgaaac gtacgacaa tactggacg cttctattt agtgattta ttattattt gccaaagtt tcgagagac cgagtttta ttcattatt gaagaccct ttaccctta gcacacata actgacatg tttttcaac gttaggaaa aaagatact gaatttagg ataactgtt acaccaggt ggaagactt gcgagtagt ccaactgta cactgcgga cctgactta tcgagaact ccgaaaaaa aatacgggt aaaaggaca gtgatttta agggcaccg acgacggag ctgaaagcg gtttggcaa aatctgctc cagcggcaa atcttccac gtaaacgcg ggcatggac ggcagctca tttagcagc ccgttagaa tctcccgac gctccaata acctcctcc gtggccacc cttcagtct gcagaatcg ctctccttc cgtcggcag gtgcgtctg tgtagcggt cactgcagc gaaccagta gataactcc tgcctacct gcacaaaac cgctcccca cgccaccat cagccctca ttgcccaca caaacatcg ctgaccaaa acccccgac acaccctcg gcgaacatt tcctcgcaa tcgtcctcc gagcaatct caggctttc acctcgcat ttcacttcc gagagtagt ttggtacca aagtactac ctacccgaa gaaccgcaa tccccttcc gctatcctc actccttca acggagatc tcatccgac gctgatgca cacaactcg gaagacata gacttttta aaatttgac gaggatccg tgggatatc acgaatttc gattacgat ctcagtagt ctgaatatc gacgggttg tcgaccaaa aaaatgcaa aagcaaata gacgccctt atagagcat aagtgtaaa caattgagc aaaaataat agcggaaaa ggtaatgct attcattta gaaaaatgg atgaaaggt catcttgat tctgatatt gctgatgaa gcagaattg gaaccttta tccgaagaa tggaccgaa gaaatgctc cgaaaacgg tcagaagaa ctccagtta atagacgcc caagggaac gttacctct cgtaacgac acccgaaga atcgaaagc agatgcgaa gacaacaac ttatctgac cacgatcaa agtccgtcc aaaagtaca acaacagaa agtcaagtc accgtgcgc tcaagcccc ttagtgcca gaaactgtg ccagtgtgc cgacagtgc cataaaaat tgccttcca tctgtaaac aatagtgtc aaaagtgct cgatctaac aataacacc gaaaatcca aaaaaagac gaaaatgat aataaagac gaagacgac tggagaccc ctccttctg atgggctta agtgcaatc aacccagcg gctagtcta gtcaaatta gatccgttt tctacacca atcccacaa ataaacgtc gcgccacca acaccagaa agctccacc aagaccgga agcacttcc gatcagaaa cagaaagac ggttcctgc agttgtcgc aatagcagg cctgaattg aataactta gaactatcc aacgaagtt gatatatca ccagacgat tcacctcaa actgaagaa catccatat cactcgtta agcagctca aacttaaca ctacgaaga tacggtaca gtttctagt ttagaacga ttaggaaca gacgaacac gaagacaac tgggatgaa cgatattcg tctgatgaa gaaaacgaa gaacgtgga ggtattgac aacgaagca ttttaccat tcatctatt aaaagttgg actgcgaaa gctggctct tttgttgcc gaaaaaatg tcgtttttt gaacgactt ggagaagat tatagaagc actgggcgg ttttttgaa agatattta aaaacagca gaaactaca gttaatgga gatgacgtc caagaagac gaaacatct ggcgctact tctggcgaa gaaatatgg ggaacaccc accagtggt ggtgaaatg gaagatccc ttgagttca ccaaattat gaaggaaaa caatcgcct tatgacggt tcaatatcg tccgacaat ggagacgac acagaactc atgatggac gagctgcta atgacccct cccatcact ggtgccatt atgagaggt cttcttcct cgcaggaca ctagaaccg ttgatagaa gaagacttt tcggagagt tgttctccc tcatcatcg ggaacccca accgagcct tccatgtct ccggaacag ggcaacgga actggcgat gttgctgac aaatgttcg actgctgta gaaaaccgg atagagact ccagagatg gggtcggca gttacgacg tccgaagcg actgctgtt ccgaaaatt catcgatcg gaaagttac cgcaagatt gtagaagcg gcggaagaa gactacgac gacgccctc ggttttttc gaccggttc aagccaaca gtgaagttc ataaatatt gagcgagta ccgcgagca aaaagtgca aaaattttt gagtttttt aacatgcgg cgcgaacgc cgaatccac caaaccatt ccggaagga aaacaatta atcaaaatt ttcagtaaa gaaggcacg atttcagac aatgttcct acgtcttat gcggaaaaa ccgctttcg cctagacga ttaaaggat aagcagatg gatcgaagg ttttggaaa cagttgagt agacgtcgt ggcaataaa atgtcaaac ctgccggcc taa
- >TC030997 RB
atgtccttt gatgtcaga catgtgtat acgaaggat atcagcggt tcatatcgt ttgctgcct cagtcgtca ggacgctgt caaaacatt ccgataagc gtcgagccg ttagatcgc gaagcagag gaagactgc cagcctata atgtgcgag agtcagtcg agcgtcgac gagatcatg agccttgat ggtctcgac aacgacttg aagaaagcc accaacaag gcgatcctg agcgagcag cagaaatat gcagatgag aacttgttc ctgcgctat ctggagctg gaccctacg gaagggccg gaagccatc cccgccgcg atgcagaga aaagcgacg gggaacaaa aacggccgg acgcccttt acgcagacg aagaagttg acgcgtgct cccaaatcc gggttcggc ttctcggtc gtctggacg catccgccg cggatagaa agggtcgag aaggggctg ccggcggag aaagcgggg attttgccg ggggattac attatcttc gtcgataaa cacaatgtc gtcatgatg ccagaaatt gatattttg aatttaatt aggtcgtat ggaagtcaa ttaacttta gaaatattt agaagaaac gcggcgaaa aacggttct gtgccaagc gtcaggcgt ttaaatagt accggaact tgcagcacg acggggctt cctataagc tctaattta gtgcaaaga cgtccctcc actgtttgt tcaacgaat acagcctca gtcgattac aacaggaga aaattgcat ttacctcaa gttactttt agtgcagag aaagccagt aataaccaa gaagaaaat agaacgaaa gccatgtat caactaata cataaagaa caacaatac gcaacaggg ttacaattt gcagttaca agattcgta tcagctctg gctgagcgg cgagactta attacacct tccgaacat aaaattctt tttcaaaat tccgaagag attcttaga gtaacagag gatatcctc gacaattta gttcacgaa gagggtgtt cataattta attcggacg tatcacgca aaattgaac gaaattaca gcagcttac agacgctat tgctcagga attaaaaaa gctgactgc gtattagca aataaaacg aaaaattcc aattcggag tttgttagg ttcttacac acgccatcg attcctaga agacggcca gacattact gcatttatt cataaacct ctcgagcat tacagggaa gttttgaaa gctttcact gttatacaa agccacaca aagcctaat catgaagac tatcctgtg ataaatcaa cttgtgcat gacttgcag cacacttat agagaaatt acagtagag gcaggactc atggaaccg atgggggaa ggccgacct ctcctatca gtccaagac ttagaaaat cgtttagtt ttcacaaag tgtaaacct ttcgtttta aataaaccc ggtcgccaa tggatattc ggaggcgat ttagggcga gtggaagga cgaaacgta cgacaatac tggacgctt ctatttagt gatttatta ttatttgcc aaagtttcg agagaccga gttttattc attattgaa gacccttta cccttagca cacataact gacatgttt ttcaacgtt aggaaaaaa gatactgaa tttaggata actgttaca ccaggtgga agacttgcg agtagtcca actgtacac tgcggacct gacttatcg agaactccg aaaaaaaat acgggtaaa aggacagtg attttaagg gcaccgacg acggagctg aaagcggtt tggcaaaat ctgctccag cggcaaatc ttccacgta aacgcgggc atggacggc agctcattt agcagcccg ttagaatct cccgacgct ccaataacc tcctccgtg gccaccctt cagtctgca gaatcgctc tccttccgt cggcaggtg cgtctgtgt agcggtcac tgcagcgaa ccagtagat aactcctgc ctacctgca caaaaccgc tccccacgc caccatcag ccctcattg cccacacaa acatcgctg accaaaacc cccgacaca ccctcggcg aacatttcc tcgcaatcg tcctccgag caatctcag gctttcacc tcgcatttc acttccgag agtagtttg gtaccaaag tactaccta cccgaagaa ccgcaatcc ccttccgct atcctcact ccttcaacg gagatctca tccgacgct gatgcacac aactcggaa gacatagac tttttaaaa tttgacgag gatccgtgg gatatcacg aatttcgat tacgatctc agtagtctg aatatcgac gggttgtcg accaaaaaa atgcaaaag caaatagac gcccttata gagcataag tgtaaacaa ttgagcaaa aataatagc ggaaaaggt aatgctatt catttagaa aaatggatg aaaggtcat cttgattct gatattgct gatgaagca gaattggaa cctttatcc gaagaatgg accgaagaa atgctccga aaacggtca gaagaactc cagttaata gacgcccaa gggaacgtt acctctcgt aacgacacc cgaagaatc gaaagcaga tgcgaagac aacaactta tctgaccac gatcaaagt ccgtccaaa agtacaaca acagaaagt caagtcacc gtgcgctca agcccctta gtgccagaa actgtgcca gtgtgccga cagtgccat aaaaattgc cttccatct gtaaacaat agtgtcaaa agtgctcga tctaacaat aacaccgaa aatccaaaa aaagacgaa aatgataat aaagacgaa gacgactgg agacccctc cttctgatg ggcttaagt gcaatcaac ccagcggct agtctagtc aaattagat ccgttttct acaccaatc ccacaaata aacgtcgcg ccaccaaca ccagaaagc tccaccaag accggaagc acttccgat cagaaacag aaagacggt tcctgcagt tgtcgcaat agcaggcct gaattgaat aacttagaa ctatccaac gaagttgat atatcacca gacgattca cctcaaact gaagaacat ccatatcac tcgttaagc agctcaaac ttaacacta cgaagatac ggtacagtt tctagttta gaacgatta ggaacagac gaacacgaa gacaactgg gatgaacga tattcgtct gatgaagaa aacgaagaa cgtggaggt attgacaac gaagcattt taccattca tctattaaa agttggact gcgaaagct ggctctttt gttgccgaa aaaatgtcg ttttttgaa cgacttgga gaagattat agaagcact gggcggttt tttgaaaga tatttaaaa acagcagaa actacagtt aatggagat gacgtccaa gaagacgaa acatctggc gctacttct ggcgaagaa atatgggga acacccacc agtggtggt gaaatggaa gatcccttg agttcacca aattatgaa ggaaaacaa tcgccttat gacggttca atatcgtcc gacaatgga gacgacaca gaactcatg atggacgag ctgctaatg acccctccc atcactggt gccattatg agaggtctt cttcctcgc aggacacta gaaccgttg atagaagaa gacttttcg gagagttgt tctccctca tcatcggga accccaacc gagccttcc atgtctccg gaacagggc aacggaact ggcgatgtt gctgacaaa tgttcgact gctgtagaa aaccggata gagactcca gagatgggg tcggcagtt acgacgtcc gaagcgact gctgttccg aaaattcat cgatcggaa agttaccgc aagattgta gaagcggcg gaagaagac tacgacgac gccctcggt tttttcgac cggttcaag ccaacagtg aagttcata aatattgag cgagtaccg cgagcaaaa agtgcaaaa atttttgag ttttttaac atgcggcgc gaacgccga atccaccaa accattccg gaaggaaaa caattaatc aaaattttc agtaaagaa ggcacgatt tcagacaat gttcctacg tcttatgcg gaaaaaccg ctttcgcct agacgatta aaggataag cagatggat cgaaggttt tggaaacag ttgagtaga cgtcgtggc aataaaatg tcaaacctg ccggcctaa
- >TC030997 RA
agattctga tgcatgcgc gaggattta cgccggtat ttaaatgaa ataacgcgg gagaaatgt tttgcgatt gattttgtg ctttggtga agtgatttt tgcgatttt ggattatta accgacccg aaccatgct gaagtggac tgtaatggc tcggcgaga ggccgacaa atccgacgc cttgcgccg tccaacggc ggatgcttc aaccagcga aacgactcc gaagagtaa taaaacgaa acgtcggca ctccagcgg ccatcggcg gtccggggg ccggcggtc gagcttggg cctcgagat ggataaaga gaaccagtt cacgtccac tccgatcaa gtccttcga ggaccatca ccaattcga cgctttgcg ggacgtcag caatctaac accgaaccg acgagtgct ctccgctaa aaaatgcag gaggagtgc gtcgccctc ctcccatag gaagcataa gaagaaaaa gctctgtct caacccctc gcacaggaa agagttcgg agagggcaa gaactattt caggccctt cgaaacggt cgactcgca cattgaggg ggtgctcgt tgacaagga cctcagtgg agaattgcg ggtgaaaag gcgcatcca ggacacgta cgcacccac cctgcccac tttcaccac ggaatactc gccctgttt catgaggga tgcccactg tctttacaa cttcacccc cgcgaaagc gacccccct ggggaagta tctagcact ggaccaaga cactcccca cccgagcaa aaaaccgaa aatcgacca cgtcagtga ttttctgca tcaaattaa tttcataac gtccccgga agacgacaa actgacttg ccccacgat ggagtccaa gtcaaccct cacattacg cgaggagtc caaagtctc gcctctagt caaaaaatt agtagatct aaggtttag tcaattaag ttgcgataa ggacacttg taaaaacaa ctcgtccct aattaacga cttatccct cgaccaaat cgtcgacgc catcctcaa ctcctcggc cgagagcgc ggccaacaa agagaacga atcgaacga aacgcacga agaaaactt aataaacac agaaaacga gcgacacgt cgccacttc ctttgatcg ctgctcttc cgactcggg cttcaagag ctcgacgac ggaatactc gcaccaact cgagggcaa tttccagtg caagtgcaa gacctccac cccgaacgc cgcactttg cgacaagac catcatcaa catcaatga aactttcaa cgaacgctg cgtggacgt gataaaccc gaggaaaag gccgtcgtc gacgcccaa cgacgacaa taacgccaa acgcgccta tttggacaa atccgagga ggacaacgt ttactgcac cctgaaacg acaacggtg catccgccg ccggagaac cgacgacga gaagaaaat gagcgccaa gaaatcgcg caaagtgga cgaacccat aatcgggga cgagtcgtt cgactccaa tatttcggg aaaaattgc cgaagataa cagcacgcc gctgaatga aacttttac gcttttgac gccagtgga taacagtcg gaggtttcg gagatgcct gttattcga atcgcccac gtcgttgtc cgaaagcgc ttcgaccac caacagcag cctccggga cgtgagagg gaccatgga tttgaacat tcgttgcga aaacgacga attatacgc caacataat tcgctgcaa ggatttgta cagacccaa cgggaagca aataaacgc ttatgtgaa ggttgcctt aagtgatcg actaggaga taatcgcag gaaacggaa cggaatatt acagcgtac cgccgttca agccgactc ctccaaacc cttcttcaa ccacacttt taaatttcc tctccagaa ggaaagcct tcaaaagcg attacatat cgaagtttg gcatcggga tcgcagctc aagaacaag cgaattttt ggggtgtat gtcctttga tgtcagaca tgtgtatac gaaggatat cagcggttc atatcgttt gctgcctca gtcgtcagg acgctgtca aaacattcc gataagcgt cgagccgtt agatcgcga agcagagga agactgcca gcctataat gtgcgagag tcagtcgag cgtcgacga gatcatgag ccttgatgg tctcgacaa cgacttgaa gaaagccac caacaaggc gatcctgag cgagcagca gaaatatgc agatgagaa cttgttcct gcgctatct ggagctgga ccctacgga agggccgga agccatccc cgccgcgat gcagagaaa agcgacggg gaacaaaaa cggccggac gccctttac gcagacgaa gaagttgac gcgtgctcc caaatccgg gttcggctt ctcggtcgt ctggacgca tccgccgcg gatagaaag ggtcgagaa ggggctgcc ggcggagaa agcggggat tttgccggg ggattacat tatcttcgt cgataaaca caatgtcgt catgatgcc agaaattga tattttgaa tttaattag gtcgtatgg aagtcaatt aactttaga aatatttag aagaaacgc ggcgaaaaa cggttctgt gccaagcgt caggcgttt aaatagtac cggaacttg cagcacgac ggggcttcc tataagctc taatttagt gcaaagacg tccctccac tgtttgttc aacgaatac agcctcagt cgattacaa caggagaaa attgcattt acctcaagt tacttttag tgcagagaa agccagtaa taaccaaga agaaaatag aacgaaagc catgtatca actaataca taaagaaca acaatacgc aacagggtt acaatttgc agttacaag attcgtatc agctctggc tgagcggcg agacttaat tacaccttc cgaacataa aattctttt tcaaaattc cgaagagat tcttagagt aacagagga tatcctcga caatttagt tcacgaaga gggtgttca taatttaat tcggacgta tcacgcaaa attgaacga aattacagc agcttacag acgctattg ctcaggaat taaaaaagc tgactgcgt attagcaaa taaaacgaa aaattccaa ttcggagtt tgttaggtt cttacacac gccatcgat tcctagaag acggccaga cattactgc atttattca taaacctct cgagcatta cagggaagt tttgaaagc tttcactgt tatacaaag ccacacaaa gcctaatca tgaagacta tcctgtgat aaatcaact tgtgcatga cttgcagca cacttatag agaaattac agtagaggc aggactcat ggaaccgat gggggaagg ccgacctct cctatcagt ccaagactt agaaaatcg tttagtttt cacaaagtg taaaccttt cgttttaaa taaacccgg tcgccaatg gatattcgg aggcgattt agggcgagt ggaaggacg aaacgtacg acaatactg gacgcttct atttagtga tttattatt atttgccaa agtttcgag agaccgagt tttattcat tattgaaga ccctttacc cttagcaca cataactga catgttttt caacgttag gaaaaaaga tactgaatt taggataac tgttacacc aggtggaag acttgcgag tagtccaac tgtacactg cggacctga cttatcgag aactccgaa aaaaaatac gggtaaaag gacagtgat tttaagggc accgacgac ggagctgaa agcggtttg gcaaaatct gctccagcg gcaaatctt ccacgtaaa cgcgggcat ggacggcag ctcatttag cagcccgtt agaatctcc cgacgctcc aataacctc ctccgtggc cacccttca gtctgcaga atcgctctc cttccgtcg gcaggtgcg tctgtgtag cggtcactg cagcgaacc agtagataa ctcctgcct acctgcaca aaaccgctc cccacgcca ccatcagcc ctcattgcc cacacaaac atcgctgac caaaacccc cgacacacc ctcggcgaa catttcctc gcaatcgtc ctccgagca atctcaggc tttcacctc gcatttcac ttccgagag tagtttggt accaaagta ctacctacc cgaagaacc gcaatcccc ttccgctat cctcactcc ttcaacgga gatctcatc cgacgctga tgcacacaa ctcggaaga catagactt tttaaaatt tgacgagga tccgtggga tatcacgaa tttcgatta cgatctcag tagtctgaa tatcgacgg gttgtcgac caaaaaaat gcaaaagca aatagacgc ccttataga gcataagtg taaacaatt gagcaaaaa taatagcgg aaaaggtaa tgctattca tttagaaaa atggatgaa aggtcatct tgattctga tattgctga tgaagcaga attggaacc tttatccga agaatggac cgaagaaat gctccgaaa acggtcaga agaactcca gttaataga cgcccaagg gaacgttac ctctcgtaa cgacacccg aagaatcga aagcagatg cgaagacaa caacttatc tgaccacga tcaaagtcc gtccaaaag tacaacaac agaaagtca agtcaccgt gcgctcaag ccccttagt gccagaaac tgtgccagt gtgccgaca gtgccataa aaattgcct tccatctgt aaacaatag tgtcaaaag tgctcgatc taacaataa caccgaaaa tccaaaaaa agacgaaaa tgataataa agacgaaga cgactggag acccctcct tctgatggg cttaagtgc aatcaaccc agcggctag tctagtcaa attagatcc gttttctac accaatccc acaaataaa cgtcgcgcc accaacacc agaaagctc caccaagac cggaagcac ttccgatca gaaacagaa agacggttc ctgcagttg tcgcaatag caggcctga attgaataa cttagaact atccaacga agttgatat atcaccaga cgattcacc tcaaactga agaacatcc atatcactc gttaagcag ctcaaactt aacactacg aagatacgg tacagtttc tagtttaga acgattagg aacagacga acacgaaga caactggga tgaacgata ttcgtctga tgaagaaaa cgaagaacg tggaggtat tgacaacga agcatttta ccattcatc tattaaaag ttggactgc gaaagctgg ctcttttgt tgccgaaaa aatgtcgtt ttttgaacg acttggaga agattatag aagcactgg gcggttttt tgaaagata tttaaaaac agcagaaac tacagttaa tggagatga cgtccaaga agacgaaac atctggcgc tacttctgg cgaagaaat atggggaac acccaccag tggtggtga aatggaaga tcccttgag ttcaccaaa ttatgaagg aaaacaatc gccttatga cggttcaat atcgtccga caatggaga cgacacaga actcatgat ggacgagct gctaatgac ccctcccat cactggtgc cattatgag aggtcttct tcctcgcag gacactaga accgttgat agaagaaga cttttcgga gagttgttc tccctcatc atcgggaac cccaaccga gccttccat gtctccgga acagggcaa cggaactgg cgatgttgc tgacaaatg ttcgactgc tgtagaaaa ccggataga gactccaga gatggggtc ggcagttac gacgtccga agcgactgc tgttccgaa aattcatcg atcggaaag ttaccgcaa gattgtaga agcggcgga agaagacta cgacgacgc cctcggttt tttcgaccg gttcaagcc aacagtgaa gttcataaa tattgagcg agtaccgcg agcaaaaag tgcaaaaat ttttgagtt ttttaacat gcggcgcga acgccgaat ccaccaaac cattccgga aggaaaaca attaatcaa aattttcag taaagaagg cacgatttc agacaatgt tcctacgtc ttatgcgga aaaaccgct ttcgcctag acgattaaa ggataagca gatggatcg aaggttttg gaaacagtt gagtagacg tcgtggcaa taaaatgtc aaacctgcc ggcctaatt atcgcaata taatagttt ttcaaaa
- >TC030997 RB
ggttcgcag tgaattgtg ataatgcgt ggcgcttag gtttagtga attgtgaac ggttgcggt tgtgactat cccgagggg gagccgaat ttgcgccac tcaacagat tgcaggata atcgggaca cggtttatc gcaagtgct cgaaaacta ttaaaatcg ggataaggg agccgctgt tttcgtcat attttcccg caggaacgc atcaatctt actccttga caaattgat ggctctccc taaacaata tcaaatgag gctctagtc ctttattat tttttaggt ttcttttta tacagtaac caagtgcca tgtactgta caatttgtg actaaatgt atcgtgatt gtttgtgac tcagtgtcc tttaattat ctcaaatca aaaaatgag ctttcccga aaattgtgt gaaagatga tcaagtttc ggggagttt aaccatcac attacgtca cctcatcta gatggaaaa aaacaagcg aatttttgg ggtgtatgt cctttgatg tcagacatg tgtatacga aggatatca gcggttcat atcgtttgc tgcctcagt cgtcaggac gctgtcaaa acattccga taagcgtcg agccgttag atcgcgaag cagaggaag actgccagc ctataatgt gcgagagtc agtcgagcg tcgacgaga tcatgagcc ttgatggtc tcgacaacg acttgaaga aagccacca acaaggcga tcctgagcg agcagcaga aatatgcag atgagaact tgttcctgc gctatctgg agctggacc ctacggaag ggccggaag ccatccccg ccgcgatgc agagaaaag cgacgggga acaaaaacg gccggacgc cctttacgc agacgaaga agttgacgc gtgctccca aatccgggt tcggcttct cggtcgtct ggacgcatc cgccgcgga tagaaaggg tcgagaagg ggctgccgg cggagaaag cggggattt tgccggggg attacatta tcttcgtcg ataaacaca atgtcgtca tgatgccag aaattgata ttttgaatt taattaggt cgtatggaa gtcaattaa ctttagaaa tatttagaa gaaacgcgg cgaaaaacg gttctgtgc caagcgtca ggcgtttaa atagtaccg gaacttgca gcacgacgg ggcttccta taagctcta atttagtgc aaagacgtc cctccactg tttgttcaa cgaatacag cctcagtcg attacaaca ggagaaaat tgcatttac ctcaagtta cttttagtg cagagaaag ccagtaata accaagaag aaaatagaa cgaaagcca tgtatcaac taatacata aagaacaac aatacgcaa cagggttac aatttgcag ttacaagat tcgtatcag ctctggctg agcggcgag acttaatta caccttccg aacataaaa ttctttttc aaaattccg aagagattc ttagagtaa cagaggata tcctcgaca atttagttc acgaagagg gtgttcata atttaattc ggacgtatc acgcaaaat tgaacgaaa ttacagcag cttacagac gctattgct caggaatta aaaaagctg actgcgtat tagcaaata aaacgaaaa attccaatt cggagtttg ttaggttct tacacacgc catcgattc ctagaagac ggccagaca ttactgcat ttattcata aacctctcg agcattaca gggaagttt tgaaagctt tcactgtta tacaaagcc acacaaagc ctaatcatg aagactatc ctgtgataa atcaacttg tgcatgact tgcagcaca cttatagag aaattacag tagaggcag gactcatgg aaccgatgg gggaaggcc gacctctcc tatcagtcc aagacttag aaaatcgtt tagttttca caaagtgta aacctttcg ttttaaata aacccggtc gccaatgga tattcggag gcgatttag ggcgagtgg aaggacgaa acgtacgac aatactgga cgcttctat ttagtgatt tattattat ttgccaaag tttcgagag accgagttt tattcatta ttgaagacc ctttaccct tagcacaca taactgaca tgtttttca acgttagga aaaaagata ctgaattta ggataactg ttacaccag gtggaagac ttgcgagta gtccaactg tacactgcg gacctgact tatcgagaa ctccgaaaa aaaatacgg gtaaaagga cagtgattt taagggcac cgacgacgg agctgaaag cggtttggc aaaatctgc tccagcggc aaatcttcc acgtaaacg cgggcatgg acggcagct catttagca gcccgttag aatctcccg acgctccaa taacctcct ccgtggcca cccttcagt ctgcagaat cgctctcct tccgtcggc aggtgcgtc tgtgtagcg gtcactgca gcgaaccag tagataact cctgcctac ctgcacaaa accgctccc cacgccacc atcagccct cattgccca cacaaacat cgctgacca aaacccccg acacaccct cggcgaaca tttcctcgc aatcgtcct ccgagcaat ctcaggctt tcacctcgc atttcactt ccgagagta gtttggtac caaagtact acctacccg aagaaccgc aatcccctt ccgctatcc tcactcctt caacggaga tctcatccg acgctgatg cacacaact cggaagaca tagactttt taaaatttg acgaggatc cgtgggata tcacgaatt tcgattacg atctcagta gtctgaata tcgacgggt tgtcgacca aaaaaatgc aaaagcaaa tagacgccc ttatagagc ataagtgta aacaattga gcaaaaata atagcggaa aaggtaatg ctattcatt tagaaaaat ggatgaaag gtcatcttg attctgata ttgctgatg aagcagaat tggaacctt tatccgaag aatggaccg aagaaatgc tccgaaaac ggtcagaag aactccagt taatagacg cccaaggga acgttacct ctcgtaacg acacccgaa gaatcgaaa gcagatgcg aagacaaca acttatctg accacgatc aaagtccgt ccaaaagta caacaacag aaagtcaag tcaccgtgc gctcaagcc ccttagtgc cagaaactg tgccagtgt gccgacagt gccataaaa attgccttc catctgtaa acaatagtg tcaaaagtg ctcgatcta acaataaca ccgaaaatc caaaaaaag acgaaaatg ataataaag acgaagacg actggagac ccctccttc tgatgggct taagtgcaa tcaacccag cggctagtc tagtcaaat tagatccgt tttctacac caatcccac aaataaacg tcgcgccac caacaccag aaagctcca ccaagaccg gaagcactt ccgatcaga aacagaaag acggttcct gcagttgtc gcaatagca ggcctgaat tgaataact tagaactat ccaacgaag ttgatatat caccagacg attcacctc aaactgaag aacatccat atcactcgt taagcagct caaacttaa cactacgaa gatacggta cagtttcta gtttagaac gattaggaa cagacgaac acgaagaca actgggatg aacgatatt cgtctgatg aagaaaacg aagaacgtg gaggtattg acaacgaag cattttacc attcatcta ttaaaagtt ggactgcga aagctggct cttttgttg ccgaaaaaa tgtcgtttt ttgaacgac ttggagaag attatagaa gcactgggc ggttttttg aaagatatt taaaaacag cagaaacta cagttaatg gagatgacg tccaagaag acgaaacat ctggcgcta cttctggcg aagaaatat ggggaacac ccaccagtg gtggtgaaa tggaagatc ccttgagtt caccaaatt atgaaggaa aacaatcgc cttatgacg gttcaatat cgtccgaca atggagacg acacagaac tcatgatgg acgagctgc taatgaccc ctcccatca ctggtgcca ttatgagag gtcttcttc ctcgcagga cactagaac cgttgatag aagaagact tttcggaga gttgttctc cctcatcat cgggaaccc caaccgagc cttccatgt ctccggaac agggcaacg gaactggcg atgttgctg acaaatgtt cgactgctg tagaaaacc ggatagaga ctccagaga tggggtcgg cagttacga cgtccgaag cgactgctg ttccgaaaa ttcatcgat cggaaagtt accgcaaga ttgtagaag cggcggaag aagactacg acgacgccc tcggttttt tcgaccggt tcaagccaa cagtgaagt tcataaata ttgagcgag taccgcgag caaaaagtg caaaaattt ttgagtttt ttaacatgc ggcgcgaac gccgaatcc accaaacca ttccggaag gaaaacaat taatcaaaa ttttcagta aagaaggca cgatttcag acaatgttc ctacgtctt atgcggaaa aaccgcttt cgcctagac gattaaagg ataagcaga tggatcgaa ggttttgga aacagttga gtagacgtc gtggcaata aaatgtcaa acctgccgg cctaattat cgcaatata atagttttt caaaa
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